CN113180745A - Oral cavity sampling swab - Google Patents
Oral cavity sampling swab Download PDFInfo
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- CN113180745A CN113180745A CN202110401538.3A CN202110401538A CN113180745A CN 113180745 A CN113180745 A CN 113180745A CN 202110401538 A CN202110401538 A CN 202110401538A CN 113180745 A CN113180745 A CN 113180745A
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- 238000005070 sampling Methods 0.000 title claims abstract description 96
- 210000000214 mouth Anatomy 0.000 title abstract description 19
- 239000000463 material Substances 0.000 claims abstract description 11
- 238000012408 PCR amplification Methods 0.000 claims abstract description 10
- 238000003753 real-time PCR Methods 0.000 claims description 15
- 239000004677 Nylon Substances 0.000 claims description 2
- 239000004743 Polypropylene Substances 0.000 claims description 2
- 239000004793 Polystyrene Substances 0.000 claims description 2
- 229920001778 nylon Polymers 0.000 claims description 2
- -1 polypropylene Polymers 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- 229920002223 polystyrene Polymers 0.000 claims description 2
- 239000011347 resin Substances 0.000 claims description 2
- 229920005989 resin Polymers 0.000 claims description 2
- 210000003128 head Anatomy 0.000 abstract description 10
- 239000003112 inhibitor Substances 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 49
- 230000003321 amplification Effects 0.000 description 27
- 238000003199 nucleic acid amplification method Methods 0.000 description 27
- 238000003752 polymerase chain reaction Methods 0.000 description 21
- 238000000034 method Methods 0.000 description 16
- 238000006243 chemical reaction Methods 0.000 description 14
- 108020004414 DNA Proteins 0.000 description 12
- 102000053602 DNA Human genes 0.000 description 12
- 239000003153 chemical reaction reagent Substances 0.000 description 7
- 238000000605 extraction Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 239000008213 purified water Substances 0.000 description 5
- 239000007850 fluorescent dye Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 239000000975 dye Substances 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000010791 quenching Methods 0.000 description 3
- 230000000171 quenching effect Effects 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 238000007400 DNA extraction Methods 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 238000007790 scraping Methods 0.000 description 2
- 102100034044 All-trans-retinol dehydrogenase [NAD(+)] ADH1B Human genes 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- 101000780453 Homo sapiens All-trans-retinol dehydrogenase [NAD(+)] ADH1B Proteins 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 108010009513 Mitochondrial Aldehyde Dehydrogenase Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000002944 PCR assay Methods 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 125000006853 reporter group Chemical group 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/02—Instruments for taking cell samples or for biopsy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Public Health (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Animal Behavior & Ethology (AREA)
- Heart & Thoracic Surgery (AREA)
- Veterinary Medicine (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- Surgery (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
Abstract
The invention provides an oral sampling swab, which comprises a handle and a sampling swab head, wherein the sampling swab head comprises a sampling head body and a bulge and/or a depression for increasing the surface area on the sampling head body, and the exterior of the sampling head body and the bulge and/or the depression are not covered by other materials. The oral sampling swab can bring out enough sample amount in the oral cavity, and simultaneously reduces the content of the brought-out PCR inhibitor. The oral sample collected by using the oral sampling swab of the present invention may be directly subjected to PCR amplification.
Description
Technical Field
The invention relates to the technical field of biology, in particular to an oral sampling swab.
Background
For molecular diagnostics, the most common clinical detection method is the fluorescent quantitative PCR method. PCR (Polymerase Chain Reaction) is an in situ ReactionA technique for amplifying specific DNA (deoxyribonucleic acid) fragments in vitro. PCR generally needs three steps of high-temperature melting reaction, annealing reaction and extension reaction when amplifying DNA, and the number of initial DNA molecules is doubled after the three steps, namely, one cycle is adopted; the multiplied DNA molecules become the template of the next cycle, the cycle is multiplied, and after 30-40 cycles, the number of DNA molecules is amplified to be about 10 of the initial value9-1010And (4) doubling. Thus, PCR can specifically amplify DNA molecules as analytes on a large scale for the purpose of analysis and testing, and thus PCR technology can be used for early diagnosis of infectious diseases, genetic diseases, tumors, and the like, and is also increasingly used in prenatal examinations and forensic examinations. Molecular detection methods based on PCR technology typically require sample processing, nucleic acid extraction and PCR reaction system construction, PCR amplification reactions, and signal detection.
The fluorescent quantitative PCR method is generally classified into a probe method and a dye method. The probe method is characterized in that a pair of primers is added and a specific fluorescent probe is added at the same time when PCR amplification is carried out, the probe is an oligonucleotide, and two ends of the oligonucleotide are respectively marked with a reporter fluorescent group and a quenching fluorescent group. When the probe is complete, the fluorescent signal emitted by the reporter group is absorbed by the quenching group; initially, the probe is bound to any single strand of DNA; during PCR amplification, the 5 '-3' -exonuclease activity of Taq enzyme cuts and degrades the probe, so that the reporter fluorescent group and the quenching fluorescent group are separated, a fluorescence monitoring system can receive a fluorescence signal, namely, one fluorescent molecule is formed when one DNA chain is amplified, and the fluorescent signal accumulation and the PCR product formation are completely synchronous. The dye method uses a fluorescent dye capable of binding to nucleic acid. The dyes can be combined on double-stranded DNA, when a template in a system is amplified, the fluorescent dyes can be effectively combined on newly synthesized double strands, along with the progress of PCR, more and more combined fluorescent dyes are combined, and fluorescent signals detected by an instrument are stronger and stronger, so that the quantitative purpose is achieved.
The PCR reaction needs a template, and the sample is obtained after the sample is extracted by a conventional oral exfoliated cell template. Most of the extraction processes of the samples are complicated and time-consuming, and researchers can realize simple extraction through formula adjustment. However, the simple extraction cannot completely remove impurities that inhibit the PCR reaction in the sample, and thus the detection rate is low.
In addition, for the sampling tools frequently used at present, such as cotton swabs, flocked swabs and the like, a large amount of useless protein and impurities can be collected in the sampling process, and the quality of a sample is influenced. If such a sampling tool is used, at least a simple sample handling process is required due to excessive sample contamination.
Therefore, there is still a great need for more efficient sampling tools and simpler and easier methods for performing PCR assays.
Disclosure of Invention
In one aspect, the present invention provides an oral sampling swab comprising a handle and a sampling swab head, the sampling swab head comprising a sampling head body, and protrusions and/or depressions on the sampling head body for increasing surface area, wherein the exterior of the sampling head body and the protrusions and/or depressions are not covered with other materials.
In another aspect, the invention also provides the use of the oral sampling swab in a detection method for collecting an oral sample and then directly performing PCR amplification.
Compared with the existing oral sampling tool, the oral sampling swab can bring out enough sample volume in the oral cavity, and simultaneously reduces the content of the brought-out PCR inhibitor. The oral cavity sample collected by the oral cavity sampling swab can be directly subjected to PCR amplification, so that a complex and lengthy DNA or RNA extraction process is avoided, and consumables for sample storage and extraction are saved. In addition, all PCR amplification reagents used in the invention can be stored at normal temperature, and refrigeration and freezing are not required.
Drawings
Fig. 1 is a schematic view of an oral sampling swab of the present invention, wherein (a), (b), (c), and (d) respectively show specific examples of the oral sampling swab of the present invention.
Fig. 2 is a partially enlarged schematic view of the swab head of the oral sampling swab of the present invention, wherein (a) (b) (c) (d) (e) (f) (g) (h) (i) respectively represent specific examples of the swab head of the oral sampling swab of the present invention.
FIG. 3A shows an amplification curve obtained by directly performing fluorescent quantitative PCR amplification on an oral sample collected by using the oral sampling swab of the present invention shown in FIG. 2 (e).
FIG. 3B shows an amplification curve obtained by directly performing fluorescent quantitative PCR amplification after collecting an oral sample using the oral sampling swab of the present invention shown in FIG. 2 (c).
FIG. 3C shows an amplification curve obtained by directly performing fluorescent quantitative PCR amplification after collecting an oral sample using the oral sampling swab of the present invention shown in FIG. 2 (d).
FIG. 3D shows an amplification curve obtained by directly performing fluorescent quantitative PCR amplification after collecting an oral sample using the oral sampling swab of the present invention shown in FIG. 2 (f).
FIG. 3E shows an amplification curve obtained by directly performing fluorescent quantitative PCR amplification on an oral sample collected by using the oral sampling swab of the present invention shown in FIG. 2 (g).
FIG. 3F shows an amplification curve obtained by directly performing fluorescent quantitative PCR amplification after collecting an oral sample using the oral sampling swab of the present invention shown in FIG. 2 (h).
FIG. 3G shows an amplification curve obtained by directly performing fluorescent quantitative PCR amplification after collecting an oral sample using the oral sampling swab of the present invention shown in FIG. 2 (i).
FIG. 4 shows an amplification curve obtained by direct fluorescent quantitative PCR amplification after collection of an oral sample using a conventional mouth swab stick.
FIG. 5 shows an amplification curve obtained by using a conventional mouth swab sampling stick to collect an oral sample, extracting DNA from the sample using a conventional kit, and then performing fluorescent quantitative PCR amplification.
Detailed Description
The invention provides an oral sampling swab, which comprises a handle and a sampling swab head, wherein the sampling swab head comprises a sampling head body and a bulge and/or a depression for increasing the surface area on the sampling head body, and the exterior of the sampling head body and the bulge and/or the depression are not covered by other materials.
Preferably, the material of the sampling head body and the projections and/or recesses may be polypropylene, polystyrene, nylon, or resin.
In a specific embodiment, the sampling head body is in the shape of a flat cuboid, and 4-25 protrusions, such as 9 protrusions, 12 protrusions, 16 protrusions, etc., are distributed on a horizontal plane of the sampling head body. In particular, the 9 protrusions are distributed in a 3 × 3 array, the 12 protrusions are distributed in a 3 × 4 array, and the 16 protrusions are distributed in a 4 × 4 array.
In another specific embodiment, the sampling head body is in the shape of a flat cuboid, and 4-25 protrusions, such as 9 protrusions, 12 protrusions, 16 protrusions and the like, are distributed on a horizontal plane of the sampling head body, and each protrusion is provided with a recess. In particular, the 9 protrusions are distributed in a 3 × 3 array, the 12 protrusions are distributed in a 3 × 4 array, and the 16 protrusions are distributed in a 4 × 4 array.
In another embodiment, the sampling head body is in the shape of a cylinder, and 32-128 protrusions, such as 45 protrusions, 72 protrusions, 98 protrusions, etc., are distributed on the side surface of the sampling head body. In particular, the 45 protrusions are distributed in a 5 × 9 array, the 72 protrusions are distributed in a 6 × 12 array, and the 98 protrusions are distributed in a 7 × 14 array.
In another specific embodiment, the sampling head body is in the shape of a cylinder, and 32 to 128 protrusions, such as 45 protrusions, 72 protrusions, 98 protrusions and the like, are distributed on the side surface of the sampling head body, and each protrusion is provided with a recess. In particular, the 45 protrusions are distributed in a 5 × 9 array, the 72 protrusions are distributed in a 6 × 12 array, and the 98 protrusions are distributed in a 7 × 14 array.
In yet another embodiment, the sampling head body is in the shape of a rectangular parallelepiped with a depression in a horizontal plane. In particular, one side of the recess is rectangular, trapezoidal or triangular.
The invention also provides the use of the oral sampling swab in a detection method for collecting an oral sample and then directly carrying out PCR amplification.
Preferably, the PCR is a fluorescent quantitative PCR.
The following examples are intended to illustrate the invention without limiting it. Example 1 oral samples were collected using an oral sampling swab of the invention and directly subjected to PCR
1. Test materials and reagents
Note: no. 1 corresponds to fig. 2(e), No. 2 corresponds to fig. 2(c), No. 3 corresponds to fig. 2(d), No. 4 corresponds to fig. 2(f), No. 5 corresponds to fig. 2(g), No. 6 corresponds to fig. 2(h), and No. 7 corresponds to fig. 2 (i).
2. Experimental procedures and results
The target fragments amplified in the PCR experiment are ADH1B and ALDH2 genes.
The detailed information of the primer probe in the PCR reaction system is shown in the following table:
(1) sample collection
Preparing 3 1.5ml centrifuge tubes, and adding 50 mul of pure water into the tubes; using 3 oral sampling swabs of the invention to respectively scrape 3 persons under the inner walls of the oral cavities at the left and right sides; one end of a sampling swab head of each of 3 oral sampling swabs is respectively placed into 3 1.5ml centrifugal tubes, the sampling swab heads are immersed in purified water, the oral sampling swabs are taken out and discarded under the condition of stirring, and 1.5ml centrifugal tube covers are covered to respectively mark a first tube and a second tube to finish sampling.
(2) Sample application
Respectively adding 40 mu l of liquid in the third sample tube into a PCR reaction tube containing a freeze-drying reagent; and covering the tube cover, marking and completing sample adding.
(3) Amplification of
The 3 tubes of labeled reaction tubes were placed on the instrument and amplification was performed according to the following pre-set procedure. Results for the oral sampling swabs of the present invention, Nos. 1-7, are shown in FIGS. 3A-3G, respectively. According to the experimental result, the method is simple and convenient to operate, the PCR amplification has an obvious S-shaped curve, and the CT value is less than or equal to 35.
Example 2 oral samples were collected using an existing mouth swab sampling wand and then directly subjected to PCR
1. Test materials and reagents
2. Experimental procedures and results
The target gene amplified in this example, the primers and the probes used were the same as those in example 1.
(1) Sample collection
Preparing 3 centrifugal tubes with the volume of 1.5mL, and adding 500 mu L of purified water into the centrifugal tubes; scraping the inner walls of the oral cavities of the left side and the right side of 3 persons by using 3 mouth swab sampling rods; one end of a sampling head of 3 sampling rods is respectively placed into 3 1.5mL centrifugal tubes, the sampling heads are immersed in purified water, the sampling rods are taken out and discarded under the condition of stirring, and 1.5mL centrifugal tube covers are covered to respectively mark a first tube and a second tube, so that sampling is completed.
(2) Sample application
Respectively adding 40 mu L of liquid in the third sample tube into the PCR reaction tube containing the freeze-drying reagent; and covering the tube cover, marking and completing sample adding.
(3) Amplification of
3 tubes of labeled reaction tubes were placed on the instrument and amplification was performed according to the following procedure, the results of which are shown in FIG. 4.
According to the experimental result, the existing mouth swab sampling rod is used for collecting the oral cavity sample, and then the fluorescence quantitative PCR is directly carried out, so that the obtained amplification S-shaped curve is not obvious, and part of Ct values cannot be interpreted. The results of the experiment were not good.
Example 3 oral samples were collected using an existing mouth swab sampling wand and sample DNA extraction followed by PCR using a conventional kit
1. Test materials and reagents
2. Experimental procedures and results
The target gene amplified in this example, the primers and the probes used were the same as those in example 1.
(1) Sample collection
Preparing 3 centrifugal tubes with the volume of 1.5mL, and adding 500 mu L of purified water into the centrifugal tubes; scraping the inner walls of the oral cavities of the left side and the right side of 3 persons by using 3 mouth swab sampling rods; put 3 sampling head one end of mouth swab sampling stick into 3 respectively in 1.5mL centrifugal tubes, the sampling head submergence is in purified water, stirs under the number, takes out the sampling stick and abandons, covers 1.5mL centrifugal tube and covers and mark (r) No. pipe respectively, accomplishes the sampling.
(2) Sample extraction
DNA extraction was performed on 3 specimens using the Tiangen DP-362 kit.
(3) Sample application
Respectively adding the extracted third sample into a PCR reaction tube containing a freeze-drying reagent; and covering a tube cover on each tube by 40 mu L, and marking to finish sample adding.
(4) Amplification of
3 tubes of labeled reaction tubes were placed on the instrument and amplification was performed according to the following procedure, the results of which are shown in FIG. 5.
According to the experimental result, after the sample is collected by adopting the existing mouth swab sampling rod, the sample is extracted by using the conventional kit, and then the fluorescent quantitative PCR amplification is carried out, so that the obvious S-shaped curve amplification is obtained, the Ct is less than or equal to 35, and the result is better. However, this process involves a sample extraction step, which is cumbersome and complicated.
Claims (10)
1. An oral sampling swab comprising a handle and a sampling swab head, the sampling swab head comprising a sampling head body, and protrusions and/or depressions on the sampling head body for increasing surface area, wherein the exterior of the sampling head body and the protrusions and/or depressions are not covered with other materials.
2. The oral sampling swab of claim 1, wherein the sampling head body and the self-material of the projections and/or depressions are a polypropylene material, a polystyrene material, nylon, resin.
3. An oral sampling swab according to claim 1 or 2, wherein the sampling head body has the shape of a flat cuboid, with 4-25 protrusions distributed on one horizontal plane, preferably with 9 protrusions distributed on one horizontal plane, the 9 protrusions being distributed in a 3 x 3 array; or 12 bulges are distributed on one horizontal plane of the device, and the 12 bulges are distributed in a 3 multiplied by 4 array; or 16 bulges are distributed on one horizontal plane of the base plate, and the 16 bulges are distributed in a 4 multiplied by 4 array.
4. An oral sampling swab according to claim 3, wherein one depression is provided on each of said protrusions.
5. An oral sampling swab according to claim 1 or 2, wherein the sampling head body is in the shape of a cylinder with 32-128 protrusions distributed on its side, preferably 45 protrusions distributed on its side, the 45 protrusions being distributed in a 5 x 9 array; or 72 bulges are distributed on the side surface of the base plate, and the 72 bulges are distributed in a 6 multiplied by 12 array; or 98 bulges are distributed on the side surface of the base plate, and the 98 bulges are distributed in a 7 multiplied by 14 array.
6. An oral sampling swab according to claim 5, wherein one depression is provided on each of said protrusions.
7. An oral sampling swab according to claim 1 or claim 2, wherein the sampling head body is shaped as a rectangular parallelepiped, having a depression in a horizontal plane.
8. An oral sampling swab according to claim 7, wherein one side of the recess is rectangular, trapezoidal or triangular.
9. Use of an oral sampling swab according to any one of claims 1 to 8 for collecting an oral sample.
10. Use according to claim 9, wherein the oral sample is used directly for PCR amplification, preferably the PCR is fluorescent quantitative PCR.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110401538.3A CN113180745A (en) | 2021-04-14 | 2021-04-14 | Oral cavity sampling swab |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110401538.3A CN113180745A (en) | 2021-04-14 | 2021-04-14 | Oral cavity sampling swab |
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| CN113180745A true CN113180745A (en) | 2021-07-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202110401538.3A Pending CN113180745A (en) | 2021-04-14 | 2021-04-14 | Oral cavity sampling swab |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113576556A (en) * | 2021-09-03 | 2021-11-02 | 中国疾病预防控制中心职业卫生与中毒控制所 | Oral mucosa cell sampling swab and sampling box |
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| US20090043224A1 (en) * | 2005-05-31 | 2009-02-12 | Aprovix Ab | Sampling System |
| US20100137741A1 (en) * | 2008-12-01 | 2010-06-03 | Oasis Diagnostics Corporation | Multi compartment body part scraping fluid collection device |
| CN101983036A (en) * | 2008-02-15 | 2011-03-02 | 3M创新有限公司 | Sample acquisition device |
| CN102573655A (en) * | 2009-06-17 | 2012-07-11 | 吉卢比有限公司 | Detection device for in vivo and/or in vitro enrichment of sample material |
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| CN111839606A (en) * | 2020-08-14 | 2020-10-30 | 广州市疾病预防控制中心(广州市卫生检验中心、广州市食品安全风险监测与评估中心、广州医科大学公共卫生研究院) | Sampling swab |
| KR20210055836A (en) * | 2019-11-07 | 2021-05-18 | 대한민국(농촌진흥청장) | Extracting apparatus for oral fluid |
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2021
- 2021-04-14 CN CN202110401538.3A patent/CN113180745A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2362445Y (en) * | 1999-02-09 | 2000-02-09 | 于建斌 | Urethra cervial externum sampling bar |
| US20020007686A1 (en) * | 1999-03-05 | 2002-01-24 | Kozak Kenneth James | Biological sampling and storage container utilizing a desiccant |
| US20090043224A1 (en) * | 2005-05-31 | 2009-02-12 | Aprovix Ab | Sampling System |
| CN101983036A (en) * | 2008-02-15 | 2011-03-02 | 3M创新有限公司 | Sample acquisition device |
| US20100137741A1 (en) * | 2008-12-01 | 2010-06-03 | Oasis Diagnostics Corporation | Multi compartment body part scraping fluid collection device |
| CN102573655A (en) * | 2009-06-17 | 2012-07-11 | 吉卢比有限公司 | Detection device for in vivo and/or in vitro enrichment of sample material |
| CN104274214A (en) * | 2013-05-31 | 2015-01-14 | 吉卢比有限公司 | Detection Device for the In Vivo and/or In Vitro Enrichment of Sample Material |
| CN108784752A (en) * | 2018-08-01 | 2018-11-13 | 苏州呼呼健康科技有限公司 | One kind swallowing formula oesophagus sampler |
| KR20210055836A (en) * | 2019-11-07 | 2021-05-18 | 대한민국(농촌진흥청장) | Extracting apparatus for oral fluid |
| CN111839606A (en) * | 2020-08-14 | 2020-10-30 | 广州市疾病预防控制中心(广州市卫生检验中心、广州市食品安全风险监测与评估中心、广州医科大学公共卫生研究院) | Sampling swab |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113576556A (en) * | 2021-09-03 | 2021-11-02 | 中国疾病预防控制中心职业卫生与中毒控制所 | Oral mucosa cell sampling swab and sampling box |
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