CN215192519U

CN215192519U - Device for establishing nerve injury erectile dysfunction model

Info

Publication number: CN215192519U
Application number: CN202023057617.7U
Authority: CN
Inventors: 汤育新; 印英豪; 戴英波; 李子太宇; 何侃成; 向亚利; 陈瀚飞; 周骏; 周益红; 叶昆
Original assignee: Fifth Affiliated Hospital of Sun Yat Sen University
Current assignee: Fifth Affiliated Hospital of Sun Yat Sen University
Priority date: 2020-12-18
Filing date: 2020-12-18
Publication date: 2021-12-17
Anticipated expiration: 2030-12-18

Abstract

The utility model relates to the establishment of an erectile dysfunction model, in particular to a device for establishing a nerve injury erectile dysfunction model, which comprises an operation clamp, wherein the tip of one clamp of the operation clamp is wrapped by a flexible sleeve, a liner hard object is arranged between the two clamps of the operation clamp, and the other clamp of the operation clamp is positioned at the lower side of the liner hard object; and a silk thread is placed on the liner hard object, the clamp wrapped with the flexible sleeve is positioned above the silk thread, and the silk thread is clamped by the two clamps. The utility model discloses a forceps holder damage effect can be guaranteed to operation pincers and liner hard thing, and adopts the mode of this operation pincers of flexible cover parcel, can avoid the most advanced tooth of operation pincers to produce the cutting action and arouse neural fracture to through operation pincers, can guarantee in the experimentation, the rat cavernosum nerve of similar silk thread causes the damage, thereby reaches the purpose of establishing neural injury nature erectile dysfunction model.

Description

Device for establishing nerve injury erectile dysfunction model

Technical Field

The utility model relates to the establishment of an erectile dysfunction model, in particular to a device for establishing a nerve injury erectile dysfunction model.

Background

Erectile Dysfunction (ED) is one of the common diseases in adult males, and it is predicted that by 2025, ED patients will reach 3.22 million worldwide. Therefore, it has been a hotspot of urological andrology concern. The results of multicenter large sample surveys done domestically show that: the ED ratio caused by pelvic surgery or trauma is about 8%. Despite the continual improvement of surgical techniques and surgical instruments, the problem of post-operative nerve injury ED remains inevitable. Mulhall JP reports an incidence of ED of about 14-90% after radical prostatectomy. The action of the phosphodiesterase-5 type inhibitor (PDE 5-Is) which Is clinically commonly used by the ED patient at present depends on nitric oxide in the smooth muscle of the corpus cavernosum penis, so that the effective rate of the ED patient after RP surgery on the PDE5-Is only 0-15%. The search for new therapeutic modalities has become a key issue for nerve-damaged ED.

However, studies to enhance nerve-damaged ED inevitably require the construction of animal models thereof. There is currently no accepted uniform molding process. Because the cavernous nerve of the rat is thin, the common forceps can not ensure the injury of the Cavernous Nerve (CN) and the success of modeling. Therefore, it is necessary to find a suitable nerve injury ED model.

SUMMERY OF THE UTILITY MODEL

To the technical problem, the utility model provides a device of erection dysfunction model of nerve injury nature establishes, and the device can guarantee cavernous nerve's damage and ensure that the model is successful.

The utility model provides a technical scheme that above-mentioned technical problem adopted does: a device for establishing a nerve injury erectile dysfunction model comprises an operating forceps, wherein the tip of one forceps jaw of the operating forceps is wrapped by a flexible sleeve, a liner hard object is arranged between the two forceps jaws of the operating forceps, and the other forceps jaw of the operating forceps is positioned on the lower side of the liner hard object; and a silk thread is placed on the liner hard object, the clamp wrapped with the flexible sleeve is positioned above the silk thread, and the silk thread is clamped by the two clamps.

Preferably, the surgical forceps are mosquito forceps.

Preferably, the flexible sleeve is a scalp needle rubber tube.

Preferably, the length of the scalp needle rubber tube is 0.5 cm.

Preferably, the cushion hard substance adopts a 1ml syringe needle.

Preferably, the 1ml syringe needle is needlepoint removed and the severed end is sanded off on an abrasive cloth.

Preferably, the length direction of the 1ml syringe needle and the length direction of the clamp form an included angle of 90 degrees.

According to the technical scheme provided by the utility model, the utility model discloses a forceps holder damage effect can be guaranteed to operation pincers and the hard thing of liner, and adopts the mode of flexible cover parcel this operation pincers, can avoid the most advanced tooth of operation pincers to produce the cutting action and arouse neural fracture to through operation pincers, can guarantee in the experimentation, the rat cavernosum nerve of similar silk thread causes the damage, thereby reaches the purpose of establishing nerve injury nature erectile dysfunction model.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a diagram of the effect of deformation and damage of the silk thread under a microscope after the silk thread is clamped by the surgical forceps for 1 minute.

Fig. 3 is a diagram of the effect of silk thread deformation and damage under a microscope after the surgical forceps hold the silk thread for 2 minutes.

FIG. 4 is a chart showing the intracavernosal pressure of SD rats in the control group monitored 1 month after the model is constructed by using the surgical forceps in the embodiment of the present invention.

Fig. 5 is a diagram of the intracavernosal pressure of the SD rat, which is monitored after 1 month of the model creation using the operating forceps.

Fig. 6 is a comparison graph of intracavernosal pressure/mean arterial pressure of SD rats of the monitoring control group and the model group after 1 month of model creation using surgical forceps in the embodiment of the present invention.

Detailed Description

The invention will be described in detail with reference to the drawings and examples, which are provided for illustration and not for limitation.

As shown in fig. 1, the utility model provides a device for establishing a nerve injury erectile dysfunction model, which comprises a pair of surgical forceps 1, wherein the tip of one forceps holder of the surgical forceps is wrapped by a flexible sleeve 2, a liner hard object 3 is arranged between the two forceps holders of the surgical forceps, and the other forceps holder of the surgical forceps is positioned at the lower side of the liner hard object; the silk thread 4 is placed on the liner hard object, the forceps clip wrapped with the flexible sleeve is positioned above the silk thread, the silk thread is clamped by the two forceps clips, the injury degree of the silk thread can be observed under a microscope by clamping the silk thread by the operation forceps, the injury of the cavernous nerve of the rat similar to the silk thread is ensured in the experimental process, and therefore the purpose of establishing the nerve injury erectile dysfunction model is achieved.

The utility model adopts the above device to establish the nerve injury erectile dysfunction model as follows: firstly, taking an operating forceps 1 such as a mosquito forceps and the like, and wrapping the tip of one forceps holder of the operating forceps by a flexible sleeve 2 so as to prevent nerve tissue from cutting and breaking in the experimental process. In the implementation process, the flexible sleeve 2 adopts a scalp needle rubber tube with the length of 0.5cm and can completely wrap the tip of the mosquito clamp. Then the pad hard object 3 is placed between two jaws of a pair of forceps, and the other jaw of the forceps is positioned at the lower side of the pad hard object; the pad hard used a 1ml syringe needle that had its tip removed and its broken end ground flat on an abrasive cloth to prevent the tip from causing tissue damage. The hard pad is convenient for the operation forceps to clamp and can ensure the damage of nerve tissues in the operation. Then, a silk thread 4 is placed on the liner hard object, and the clamp wrapped with the flexible sleeve is positioned above the silk thread; and then, the silk thread is clamped by the two forceps jaws of the surgical forceps intermittently, the damage degree of the silk thread is observed under a microscope, and the length direction of the 1ml syringe needle and the length direction of the forceps form an included angle of 90 degrees, so that the silk thread is ensured to be damaged, as shown in fig. 2 and 3. Therefore, the utility model discloses an adopt the neural mode of silk thread simulation rat cavernosum, can guarantee to cause the damage to rat cavernosum nerve at the experimentation to establish nerve injury nature erection dysfunction model, ensure that the model is successfully made. The following are detailed examples.

Examples

1. Experimental animals: 20 SPF SD rats (300-350 g at 10-12 weeks old) were randomly divided into a control group and a construction group (10 rats/group);

2. experimental equipment: conventional surgical instruments (scalpels, tissue scissors, wire scissors, curved forceps, rat abdominal distractors, forceps); 1-0 silk thread; an electric knife; ophthalmic micro-forceps (elbow 14cm wide 0.15mm, suzhou shi qiang); mosquito forceps (mosquito hemostats-straight/1.0 mm wide/12.5 cm, HARTMAN); an information biological signal collecting and processing system (BL-420S, union of Dow.).

3. Molding: the conventional instruments used in the operation process are sterilized before operation for standby. Injecting anesthetic into abdominal cavity, fixing rat on operating table in supine position after rat anesthesia is stable, removing hair from abdomen, sterilizing with iodophor, spreading towel, and removing iodine with 75% alcohol. The skin is incised on the median line of the lower abdomen of the rat by a scalpel, the size of the incision is about 4cm, and then subcutaneous fat, muscles and peritoneum are incised layer by an electrotome to enter the abdominal cavity. The abdominal cavity was opened with a rat abdominal spreader to expose the surgical field, the rat prostate was found, the rat pelvic ganglia and cavernous nerves were found along both sides of the prostate, and then the cavernous nerves were freed by 1cm with a micro-instrument. A 1ml syringe needle with a needle tip removed and a broken end ground on abrasive cloth is lubricated by paraffin oil and then placed below the cavernous nerve, and an angle of 30 degrees is kept between the syringe needle and the long axis of the cavernous nerve; the tip of one of the forceps jaws of the mosquito forceps is wrapped by a scalp needle rubber tube with the length of 0.5cm, the tip of the mosquito forceps is used for simultaneously clamping the cavernous nerve and the needle head, the tip of the mosquito forceps wrapped by the rubber tube is arranged above the cavernous nerve, the tip of the other forceps jaw without the wrapping is arranged below the needle head of a 1ml syringe, the position of the needle head is adjusted after the forceps jaws are lightly clamped, the needle head and the mosquito forceps form an angle of 90 degrees, then the forceps jaws of 1 tooth are clamped on the mosquito forceps, and the time is counted for 1 minute. After clamping, slightly loosening the mosquito clamp, withdrawing the mosquito clamp and the 1ml needle head, and clamping the opposite cavernous nerve in the same way. After no active bleeding and no foreign objects are detected in the operation area of the abdomen of the rat, the gauze and the operation instrument are checked, the suture is carried out in a layering way, and the 75 percent alcohol is disinfected after the skin incision is discontinuously sutured. Control rats were not treated at all;

4. evaluation of erectile function: monitoring intracavernosal pressure and mean arterial pressure of the contrast group and the model-building group SD rats by using an information biological signal acquisition and processing system after model building for 1 month, wherein the monitoring results are shown in figure 4, figure 5 and figure 6.

Therefore, the modeling method of the utility model is applied to SD rats, which can ensure the injury of cavernous nerves and the success of modeling, thereby providing a uniform modeling method for erectile dysfunction with nerve injury.

Claims

1. A device for establishing a nerve injury erectile dysfunction model comprises an operating forceps, and is characterized in that: the tip of one forceps holder of the surgical forceps is wrapped by a flexible sleeve, a hard gasket is arranged between the two forceps holders of the surgical forceps, and the other forceps holder of the surgical forceps is positioned on the lower side of the hard gasket; and a silk thread is placed on the liner hard object, the clamp wrapped with the flexible sleeve is positioned above the silk thread, and the silk thread is clamped by the two clamps.

2. The apparatus for modeling erectile dysfunction according to claim 1, wherein: the operating forceps adopt mosquito-type forceps.

3. The apparatus for modeling erectile dysfunction according to claim 1, wherein: the flexible sleeve adopts a scalp needle rubber tube.

4. The apparatus for modeling erectile dysfunction according to claim 3, wherein: the length of the scalp needle rubber tube is 0.5 cm.

5. The apparatus for modeling erectile dysfunction according to claim 1, wherein: the pad was hard using a 1ml syringe needle.

6. The apparatus for modeling erectile dysfunction according to claim 5, wherein: the 1ml syringe needle had the needle tip removed and the broken end ground flat on an abrasive cloth.

7. The apparatus for modeling erectile dysfunction according to claim 6, wherein: the length direction of the 1ml syringe needle and the length direction of the forceps holder form an included angle of 90 degrees.