CN112603576A - Device and method for establishing nerve injury erectile dysfunction model - Google Patents

Abstract

The invention relates to the erection of an erectile dysfunction model, in particular to a device and a method for establishing a nerve injury erectile dysfunction model, wherein the method comprises the steps of taking an operating forceps, and wrapping the tip of one forceps holder of the operating forceps by a flexible sleeve; placing the pad hard object between two jaws of a surgical clamp, wherein the other jaw of the surgical clamp is positioned at the lower side of the pad hard object; placing a silk thread on the liner hard object, and positioning the clamp wrapped with the flexible sleeve above the silk thread; the wire was held between the two jaws of the forceps and the degree of damage to the wire was observed under a microscope. The invention can ensure the damage effect of the forceps holder through the operation forceps and the liner hard object, can avoid the nerve fracture caused by the cutting action of the teeth at the tip of the operation forceps on the tissues by adopting the mode of wrapping the operation forceps by the flexible sleeve, and can ensure that the cavernous nerve of the rat similar to silk threads causes the damage in the experimental process through operating the operation forceps, thereby achieving the purpose of establishing the nerve injury erectile dysfunction model.

Description

Device and method for establishing nerve injury erectile dysfunction model

Technical Field

The invention relates to the establishment of an erectile dysfunction model, in particular to a device and a method for establishing a nerve injury erectile dysfunction model.

Background

Erectile Dysfunction (ED) is one of the common diseases in adult males, and it is predicted that by 2025, ED patients will reach 3.22 million worldwide. Therefore, it has been a hotspot of urological andrology concern. The results of multicenter large sample surveys done domestically show that: the ED ratio caused by pelvic surgery or trauma is about 8%. Despite the continual improvement of surgical techniques and surgical instruments, the problem of post-operative nerve injury ED remains inevitable. Mulhall JP reports an incidence of ED of about 14-90% after radical prostatectomy. The action of the phosphodiesterase-5 type inhibitor (PDE 5-Is) which Is clinically commonly used by the ED patient at present depends on nitric oxide in the smooth muscle of the corpus cavernosum penis, so that the effective rate of the ED patient after RP surgery on the PDE5-Is only 0-15%. The search for new therapeutic modalities has become a key issue for nerve-damaged ED.

However, studies to enhance nerve-damaged ED inevitably require the construction of animal models thereof. There is currently no accepted uniform molding process. Because the cavernous nerve of the rat is thin, the common forceps can not ensure the injury of the Cavernous Nerve (CN) and the success of modeling. Therefore, it is necessary to find a suitable nerve injury ED model.

Disclosure of Invention

Aiming at the technical problems, the invention provides a device for establishing a nerve injury erectile dysfunction model, which can ensure the injury of cavernous nerves and the success of modeling.

The technical scheme adopted by the invention for solving the technical problems is as follows: a device for establishing a nerve injury erectile dysfunction model comprises an operating forceps, wherein the tip of one forceps jaw of the operating forceps is wrapped by a flexible sleeve, a liner hard object is arranged between the two forceps jaws of the operating forceps, and the other forceps jaw of the operating forceps is positioned on the lower side of the liner hard object; and a silk thread is placed on the liner hard object, the clamp wrapped with the flexible sleeve is positioned above the silk thread, and the silk thread is clamped by the two clamps.

Preferably, the surgical forceps are mosquito forceps.

Preferably, the flexible sleeve is a scalp needle rubber tube.

Preferably, the length of the scalp needle rubber tube is 0.5 cm.

Preferably, the cushion hard substance adopts a 1ml syringe needle.

Preferably, the 1ml syringe needle is needlepoint removed and the severed end is sanded off on an abrasive cloth.

Preferably, the length direction of the 1ml syringe needle and the length direction of the clamp form an included angle of 90 degrees.

The invention also provides a method for establishing the nerve injury erectile dysfunction model, which comprises the following steps:

(1) taking a surgical clamp, and wrapping the tip of one forceps holder of the surgical clamp by using a flexible sleeve;

(2) placing the pad hard object between two clamp jaws of the mosquito forceps, wherein the other clamp jaw of the surgical forceps is positioned at the lower side of the pad hard object;

(3) placing a silk thread on the liner hard object, and positioning the clamp wrapped with the flexible sleeve above the silk thread;

(4) the wire was held between the two jaws of the forceps and the degree of damage to the wire was observed under a microscope.

According to the technical scheme, the forceps holder can guarantee the injury effect of the forceps holder through the operation forceps and the liner hard object, the operation forceps are wrapped in the flexible sleeve, the phenomenon that the teeth at the tip of the operation forceps cut tissues to cause nerve breakage can be avoided, and the operation forceps can guarantee that the cavernous body nerves of the rat similar to silk threads cause injury in the experimental process, so that the aim of establishing the nerve injury erectile dysfunction model is fulfilled.

Drawings

FIG. 1 is a schematic diagram of the structure of the apparatus of the present invention.

FIG. 2 is a diagram showing the effect of the deformation injury of the silk thread under a microscope after the silk thread is held by the forceps for 1 minute.

Fig. 3 is a graph showing the effect of thread deformation damage under a microscope after the forceps of the present invention hold the thread for 2 minutes.

FIG. 4 is a graph showing the intracavernosal pressure of SD rats in the control group monitored 1 month after the use of surgical clips in the example of the present invention.

FIG. 5 is a graph of intracavernosal pressure of SD rats monitored after 1 month of molding using surgical forceps in accordance with an embodiment of the present invention.

FIG. 6 is a graph showing the intracavernosal pressure/mean arterial pressure of SD rats in the control group and the model group monitored 1 month after the model creation using surgical forceps in the example of the present invention.

Detailed Description

The invention will now be described in detail with reference to the drawings and examples, wherein the illustrations and descriptions of the invention are intended to illustrate, but not limit the invention.

Referring to fig. 1, the invention provides a device for establishing a nerve injury erectile dysfunction model, which comprises a pair of surgical forceps 1, wherein the tip of one jaw of the surgical forceps is wrapped by a flexible sleeve 2, a liner hard object 3 is arranged between the two jaws of the surgical forceps, and the other jaw of the surgical forceps is positioned at the lower side of the liner hard object; the silk thread 4 is placed on the liner hard object, the forceps clip wrapped with the flexible sleeve is positioned above the silk thread, the silk thread is clamped by the two forceps clips, the injury degree of the silk thread can be observed under a microscope by clamping the silk thread by the operation forceps, the injury of the cavernous nerve of the rat similar to the silk thread is ensured in the experimental process, and therefore the purpose of establishing the nerve injury erectile dysfunction model is achieved.

The method for establishing the nerve injury erectile dysfunction model by adopting the device comprises the following steps: firstly, taking an operating forceps 1 such as a mosquito forceps and the like, and wrapping the tip of one forceps holder of the operating forceps by a flexible sleeve 2 so as to prevent nerve tissue from cutting and breaking in the experimental process. In the implementation process, the flexible sleeve 2 adopts a scalp needle rubber tube with the length of 0.5cm and can completely wrap the tip of the mosquito clamp. Then the pad hard object 3 is placed between two jaws of a pair of forceps, and the other jaw of the forceps is positioned at the lower side of the pad hard object; the pad hard used a 1ml syringe needle that had its tip removed and its broken end ground flat on an abrasive cloth to prevent the tip from causing tissue damage. The hard pad is convenient for the operation forceps to clamp and can ensure the damage of nerve tissues in the operation. Then, a silk thread 4 is placed on the liner hard object, and the clamp wrapped with the flexible sleeve is positioned above the silk thread; and then, the silk thread is clamped by the two forceps jaws of the surgical forceps intermittently, the damage degree of the silk thread is observed under a microscope, and the length direction of the 1ml syringe needle and the length direction of the forceps form an included angle of 90 degrees, so that the silk thread is ensured to be damaged, as shown in fig. 2 and 3. Therefore, the invention can ensure that the cavernous nerves of the rat can be damaged in the experimental process by adopting a mode of simulating the cavernous nerves of the rat by silk threads, thereby establishing a nerve-damaged erectile dysfunction model and ensuring the success of modeling. The following are detailed examples.

Examples

1. Experimental animals: 20 SPF SD rats (300-350 g at 10-12 weeks old) were randomly divided into a control group and a construction group (10 rats/group);

2. experimental equipment: conventional surgical instruments (scalpels, tissue scissors, wire scissors, curved forceps, rat abdominal distractors, forceps); 1-0 silk thread; an electric knife; ophthalmic micro-forceps (elbow 14cm wide 0.15mm, suzhou shi qiang); mosquito forceps (mosquito hemostats-straight/1.0 mm wide/12.5 cm, HARTMAN); an information biological signal collecting and processing system (BL-420S, union of Dow.).

3. Molding: the conventional instruments used in the operation process are sterilized before operation for standby. Injecting anesthetic into abdominal cavity, fixing rat on operating table in supine position after rat anesthesia is stable, removing hair from abdomen, sterilizing with iodophor, spreading towel, and removing iodine with 75% alcohol. The skin is incised on the median line of the lower abdomen of the rat by a scalpel, the size of the incision is about 4cm, and then subcutaneous fat, muscles and peritoneum are incised layer by an electrotome to enter the abdominal cavity. The abdominal cavity was opened with a rat abdominal spreader to expose the surgical field, the rat prostate was found, the rat pelvic ganglia and cavernous nerves were found along both sides of the prostate, and then the cavernous nerves were freed by 1cm with a micro-instrument. A 1ml syringe needle with a needle tip removed and a broken end ground on abrasive cloth is lubricated by paraffin oil and then placed below the cavernous nerve, and an angle of 30 degrees is kept between the syringe needle and the long axis of the cavernous nerve; the tip of one of the forceps jaws of the mosquito forceps is wrapped by a scalp needle rubber tube with the length of 0.5cm, the tip of the mosquito forceps is used for simultaneously clamping the cavernous nerve and the needle head, the tip of the mosquito forceps wrapped by the rubber tube is arranged above the cavernous nerve, the tip of the other forceps jaw without the wrapping is arranged below the needle head of a 1ml syringe, the position of the needle head is adjusted after the forceps jaws are lightly clamped, the needle head and the mosquito forceps form an angle of 90 degrees, then the forceps jaws of 1 tooth are clamped on the mosquito forceps, and the time is counted for 1 minute. After clamping, slightly loosening the mosquito clamp, withdrawing the mosquito clamp and the 1ml needle head, and clamping the opposite cavernous nerve in the same way. After no active bleeding and no foreign objects are detected in the operation area of the abdomen of the rat, the gauze and the operation instrument are checked, the suture is carried out in a layering way, and the 75 percent alcohol is disinfected after the skin incision is discontinuously sutured. Control rats were not treated at all;

4. evaluation of erectile function: monitoring intracavernosal pressure and mean arterial pressure of the contrast group and the model-building group SD rats by using an information biological signal acquisition and processing system after model building for 1 month, wherein the monitoring results are shown in figure 4, figure 5 and figure 6.

Therefore, the modeling method provided by the invention is applied to SD rats, the injury of cavernous nerves can be guaranteed, and the success of modeling can be guaranteed, so that a uniform modeling method for the nerve injury erectile dysfunction is provided.

Claims (8)

1. A device for establishing a nerve injury erectile dysfunction model comprises an operating forceps, and is characterized in that: the tip of one forceps holder of the surgical forceps is wrapped by a flexible sleeve, a hard gasket is arranged between the two forceps holders of the surgical forceps, and the other forceps holder of the surgical forceps is positioned on the lower side of the hard gasket; and a silk thread is placed on the liner hard object, the clamp wrapped with the flexible sleeve is positioned above the silk thread, and the silk thread is clamped by the two clamps.

2. The apparatus for modeling erectile dysfunction according to claim 1, wherein: the operating forceps adopt mosquito-type forceps.

3. The apparatus for modeling erectile dysfunction according to claim 1, wherein: the flexible sleeve adopts a scalp needle rubber tube.

4. The apparatus for modeling erectile dysfunction according to claim 3, wherein: the length of the scalp needle rubber tube is 0.5 cm.

5. The apparatus for modeling erectile dysfunction according to claim 1, wherein: the pad was hard using a 1ml syringe needle.

6. The apparatus for modeling erectile dysfunction according to claim 5, wherein: the 1ml syringe needle had the needle tip removed and the broken end ground flat on an abrasive cloth.

7. The apparatus for modeling erectile dysfunction according to claim 6, wherein: the length direction of the 1ml syringe needle and the length direction of the forceps holder form an included angle of 90 degrees.

8. A method of modeling neuro-traumatic erectile dysfunction according to the apparatus of any one of claims 1 to 7, comprising the steps of:

(1) taking a surgical clamp, and wrapping the tip of one forceps holder of the surgical clamp by using a flexible sleeve;

(2) placing the pad hard object between two jaws of the surgical forceps, wherein the other jaw of the surgical forceps is positioned at the lower side of the pad hard object;

(3) placing a silk thread on the liner hard object, and positioning the clamp wrapped with the flexible sleeve above the silk thread;

(4) the wire was held between the two jaws of the forceps and the degree of damage to the wire was observed under a microscope.

Images