CN215179869U

CN215179869U - Micro-fluidic chip for virus electrical impedance real-time monitoring

Info

Publication number: CN215179869U
Application number: CN202120410104.5U
Authority: CN
Inventors: 姚佳烽; 刘凯
Original assignee: Jiangsu Jilun Medical Intelligent Technology Co ltd
Current assignee: Jiangsu Jilun Medical Intelligent Technology Co ltd
Priority date: 2021-02-24
Filing date: 2021-02-24
Publication date: 2021-12-14
Anticipated expiration: 2031-02-24

Abstract

The utility model discloses a micro-fluidic chip for virus electrical impedance real-time monitoring, which comprises a chip base body, a shielding electrode ring, a sensor array and a shielding electrode, wherein the upper surface of the chip base body comprises a liquid storage tank, a shielding electrode ring groove and a round electrode groove, the shielding electrode ring groove and the round electrode groove are embedded in the liquid storage tank, the lower surface of the chip base body comprises a strip-shaped electrode groove and a shielding electrode groove, the sensor array is formed by connecting the round electrode and the strip-shaped electrode, the enrichment and real-time monitoring of virus infected cells are realized, the detection efficiency is improved by single step, the cell infected by the virus is monitored in real time, the death of the virus or the cell in the process of transferring or processing the cell by the traditional sampling method is avoided, the accuracy of virus detection is greatly improved, and various detection liquids can be injected simultaneously to detect the virus, so that the reliability of virus detection is improved.

Description

Micro-fluidic chip for virus electrical impedance real-time monitoring

Technical Field

The utility model relates to a micro-fluidic chip, in particular to micro-fluidic chip for virus electrical impedance real-time supervision belongs to micro-fluidic chip technical field.

Background

The detection methods of the coronavirus mainly comprise a nucleic acid detection method and an antibody detection method. The nucleic acid detection process comprises a plurality of steps of sample treatment, nucleic acid extraction, PCR detection and the like, and the average detection time is long and needs 2-3 hours. The method is used for directly detecting virus nucleic acid in a collected specimen, has strong specificity and relatively high sensitivity, is a current main detection means, detects the antibody level in human blood by antibody detection, has a detection window period, can be used for auxiliary diagnosis of negative cases of nucleic acid detection, and can also be used for investigation and screening of cases, but cannot replace a nucleic acid detection method. Since the major determinant of coronavirus infectivity is the S protein, which binds to membrane receptors on host cells, mediating fusion of the virus and cell membranes, it is contemplated that viruses can be detected by detecting cells infected with coronavirus.

The Bio Impedance Spectroscopy (BIS) technology realizes real-time detection of coronavirus by detecting the relation between the dielectric property of a sample and an alternating current electric field excitation signal. The detection method has the advantages of no mark, simple operation, high detection speed and the like. The BIS technique obtains an impedance spectrum of a sample by sweeping a frequency of the sample to be detected, thereby analyzing components of the sample and detecting whether the sample contains cells infected with coronavirus. However, BIS presents two problems in the detection of coronaviruses: firstly, the concentration of infected coronavirus cells in a sample affects the measurement result of BIS, thereby affecting the analysis of sample components; secondly, the coronavirus is hosted in the host cell, and the ultrahigh frequency excitation signal is required to penetrate through the cell membrane to detect whether the coronavirus is parasitized by the virus, however, the ultrahigh frequency BIS has extremely high requirements on a detection chip and a peripheral circuit. Aiming at the two problems, a method using ultra-high frequency impedance spectroscopy (uf-BIS) is provided to realize real-time detection of the new coronavirus. The method is characterized in that the cells possibly infected with coronavirus are enriched by dielectrophoresis, and then the cells obtained by enrichment are subjected to uf-BIS detection, so that real-time coronavirus detection is realized.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a micro-fluidic chip for virus electrical impedance real-time supervision to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a micro-fluidic chip for virus electrical impedance real-time supervision, micro-fluidic chip comprises chip base member, shielding electrode circle, sensor array and shielding electrode, the upper surface of chip base member contains reservoir, shielding electrode circle groove and circle electrode groove, shielding electrode circle groove and circle electrode inslot are in the reservoir, the lower surface of chip base member contains strip electrode groove and shielding electrode groove, the sensor array is formed by round electrode and strip electrode link to each other.

As a preferred technical scheme of the utility model, the quantity of reservoir is six groups, and six groups the shape of reservoir is the same, six groups reservoir evenly distributed is in the upper surface of chip base member.

As an optimal technical scheme of the utility model, the quantity of shielding electrode circle is six groups, and six groups shielding electrode circle is identical, six groups shielding electrode circle evenly distributed is in the inside in the shielding electrode circle groove that corresponds the reservoir.

As an optimized technical scheme of the utility model, round electrode and strip electrode are connected with the round electrode groove and the strip electrode groove that correspond respectively.

As an optimal technical scheme of the utility model, be located two of both sides the strip electrode is for richening the collecting electrode, is located middle one the strip electrode is detecting electrode.

As a preferred technical scheme of the utility model, the diameter of circle electrode and the diameter of strip electrode are 10 mu m.

As a preferred technical scheme of the utility model, shielding electrode passes through adhesive and sealant and is connected with the shielding electrode groove, shielding electrode links to each other with the inside of shielding electrode circle at the chip base member, just shielding electrode is at the inside ground connection of chip base member.

As an optimized technical scheme of the utility model, the detecting electrode passes through the sedimentary metal wire of base plate surface and links to each other with external impedance analysis appearance.

Compared with the prior art, the beneficial effects of the utility model are as follows:

1. the utility model discloses on a chip, realized the enrichment and the real-time supervision of virus infection cell, it is consuming time to be long originally, and multistep virus testing process shortens to several minutes, and single step has promoted detection efficiency.

2. The utility model discloses carry out real-time supervision to the cell of being infected by the virus, avoided traditional sampling method to shift or handle the death of cell in-process virus or cell, promoted the rate of accuracy that the virus detected greatly.

3. The utility model discloses have six reservoir, can pour into multiple detection liquid simultaneously and carry out the detection of virus, increased the reliability that the virus detected.

4. The utility model discloses a detection electrode directly links to each other with outside impedance analysis appearance, but the state of real-time observation by virus infection cell possess certain scientific research value.

5. The utility model discloses use the hyperfrequency to detect, distributed shielding electrode around enrichment electrode and detecting electrode, improved the rate of accuracy that detects.

Drawings

Fig. 1 is a perspective view 1 of the virus real-time monitoring microfluidic chip of the present invention;

FIG. 2 is a perspective view of the real-time virus monitoring microfluidic chip of the present invention, FIG. 2;

FIG. 3 is a perspective view of a core substrate part of a microfluidic chip for real-time monitoring of hand virus according to the present invention;

FIG. 4 is a perspective view of a core substrate part of a microfluidic chip for real-time monitoring of hand virus according to the present invention;

fig. 5 is a perspective view of the sensor array part of the hand virus real-time monitoring micro-fluidic chip of the present invention.

In the figure: 1. a chip substrate; 2. a shielding electrode ring; 3. an array of sensors; 4. a shield electrode; 5. a liquid storage tank; 6. a shield electrode ring slot; 7. a circular electrode groove; 8. a strip-shaped electrode groove; 9. a shield electrode tank; 10. a circular electrode; 11. a strip electrode; 12. an enrichment electrode; 13. and a detection electrode.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-5, the present invention provides a technical solution of a microfluidic chip for real-time monitoring of viral impedance:

as shown in fig. 1 to 5, the microfluidic chip is composed of a chip substrate 1, a shielding electrode ring 2, a sensor array 3 and a shielding electrode 4, wherein the upper surface of the chip substrate 1 includes a liquid storage tank 5, a shielding electrode ring tank 6 and a circular electrode tank 7, the shielding electrode ring tank 6 and the circular electrode tank 7 are embedded in the liquid storage tank 5, the lower surface of the chip substrate 1 includes a strip-shaped electrode tank 8 and a shielding electrode tank 9, and the sensor array 3 is formed by connecting a circular electrode 10 and a strip-shaped electrode 11.

According to the figure 1 and figure 5, the number of the liquid storage tanks 5 is six, the six groups of liquid storage tanks 5 are in the same shape, the six groups of liquid storage tanks 5 are uniformly distributed on the upper surface of the chip base body 1, the number of the shielding electrode rings 2 is six, the six groups of shielding electrode rings 2 are completely the same, the six groups of shielding electrode rings 2 are uniformly distributed in the shielding electrode ring grooves 6 of the corresponding liquid storage tanks 5, a plurality of detection liquids can be injected simultaneously for virus detection, the virus detection reliability is increased, the circular electrodes 10 and the strip electrodes 11 are respectively connected with the corresponding circular electrode grooves 7 and strip electrode grooves 8, the two strip electrodes 11 positioned at two sides are enrichment electrodes 12, one strip electrode 11 positioned in the middle is a detection electrode 13, the diameter of the circular electrode 10 and the diameter of the strip electrode 11 are both 10 μm, the shielding electrode 4 is connected with the shielding electrode grooves 9 through an adhesive and a sealant, shielding electrode 4 links to each other at the inside of chip base member 1 with shielding electrode circle 2, and shielding electrode 4 is at the inside ground connection of chip base member 1, and detecting electrode 13 passes through the sedimentary metal wire of base plate surface and links to each other with external impedance analysis appearance, has improved the rate of accuracy that detects, can observe the state by virus infection cell in real time simultaneously, possess certain scientific research value, and the practicality is strong.

When specifically using, the utility model relates to a micro-fluidic chip for virus electrical impedance real-time supervision, through combining Polydimethylsiloxane (PDMS) preparation shaping chip base member 1 with soft lithography, circular electrode groove 7 is connected to rethread binder and sealant circle electrode 10 in proper order, shielding electrode 4 connects shielding electrode groove 9, enrichment electrode 12 and detection electrode 13 are connected to strip electrode groove 8, shield electrode circle 2 evenly distributed in the inside that corresponds the shielding electrode circle groove 6 of reservoir 5 with six groups through binder and sealant, detection electrode 13 links to each other with external impedance analysis appearance through the metal wire of chip base member 1 surface deposit, it can to pour into the detection liquid and carry out the detection of virus.

To sum up, the utility model realizes the enrichment and real-time monitoring of virus infected cells, shortens the original long and time-consuming multi-step virus detection process to several minutes, and improves the detection efficiency by one step; the cell infected by the virus is monitored in real time, so that the death of the virus or the cell in the process of transferring or treating the cell by the traditional sampling method is avoided, and the accuracy of virus detection is greatly improved; the utility model has six liquid storage tanks, can inject a plurality of detection liquids to detect the virus, and increases the reliability of virus detection; the detection electrode is directly connected with an external impedance analyzer, so that the state of the virus-infected cell can be observed in real time, and certain scientific research value is achieved; and by adopting ultrahigh frequency detection, shielding electrodes are distributed around the enrichment electrode and the detection electrode, so that the detection accuracy is improved.

In the description of the present invention, it should be understood that the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.

In the present invention, unless otherwise explicitly specified or limited, for example, it may be fixedly connected, detachably connected, or integrated; can be mechanically or electrically connected; they may be directly connected or indirectly connected through an intermediate medium, and may be connected through the inside of two elements or in an interaction relationship between two elements, unless otherwise specifically defined, and the specific meaning of the above terms in the present invention will be understood by those skilled in the art according to specific situations.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a micro-fluidic chip for viral electrical impedance real-time supervision, its characterized in that, micro-fluidic chip comprises chip base member (1), shielding electrode circle (2), sensor array (3) and shielding electrode (4), the upper surface of chip base member (1) contains reservoir (5), shielding electrode circle groove (6) and round electrode groove (7), embedded in reservoir (5) in shielding electrode circle groove (6) and round electrode groove (7), the lower surface of chip base member (1) contains strip electrode groove (8) and shielding electrode groove (9), sensor array (3) are formed by round electrode (10) and strip electrode (11) link to each other.

2. The microfluidic chip for real-time monitoring of viral electrical impedance according to claim 1, wherein: the number of the liquid storage tanks (5) is six, the six liquid storage tanks (5) are identical in shape, and the six liquid storage tanks (5) are uniformly distributed on the upper surface of the chip base body (1).

3. The microfluidic chip for real-time monitoring of viral electrical impedance according to claim 1, wherein: the number of the shielding electrode rings (2) is six, the six groups of the shielding electrode rings (2) are completely the same, and the six groups of the shielding electrode rings (2) are uniformly distributed in the shielding electrode ring grooves (6) of the corresponding liquid storage tanks (5).

4. The microfluidic chip for real-time monitoring of viral electrical impedance according to claim 1, wherein: the round electrode (10) and the strip electrode (11) are respectively connected with the corresponding round electrode groove (7) and the strip electrode groove (8).

5. The microfluidic chip for real-time monitoring of viral electrical impedance according to claim 1, wherein: the two strip-shaped electrodes (11) positioned at two sides are enrichment electrodes (12), and the strip-shaped electrode (11) positioned in the middle is a detection electrode (13).

6. The microfluidic chip for real-time monitoring of viral electrical impedance according to claim 1, wherein: the diameter of the round electrode (10) and the diameter of the strip-shaped electrode (11) are both 10 micrometers.

7. The microfluidic chip for real-time monitoring of viral electrical impedance according to claim 1, wherein: the shielding electrode (4) is connected with the shielding electrode groove (9) through an adhesive and a sealant, the shielding electrode (4) is connected with the shielding electrode ring (2) in the chip base body (1), and the shielding electrode (4) is grounded in the chip base body (1).

8. The microfluidic chip for real-time monitoring of viral electrical impedance according to claim 5, wherein: and the detection electrode (13) is connected with an external impedance analyzer through a metal wire deposited on the surface of the substrate.