CN101738418A - Microfluidic chip system integrating cell operation and detection - Google Patents
Microfluidic chip system integrating cell operation and detection Download PDFInfo
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Abstract
The invention provides a microfluidic chip system integrating cell operation and detection and relates to a device for cell manipulation and detection in early diagnosis of diseases. The microfluidic chip system mainly comprises an alternating current signal power supply, a microchip, an integrated circuit control unit and a computer. The microfluidic chip system is characterized in that: the microchip is formed by bonding polydimethylsiloxane cover glass and a glass substrate, wherein the polydimethylsiloxane cover glass is provided with pipes; the glass substrate is deposited with a cell operation unit electrode and an impedance detection unit electrode; and the integrated circuit control unit is an external circuit control unit, namely, an integrated micro-control circuit board. The microfluidic chip system integrating the cell operation and detection is characterized by high detection sensitivity, good stability, flexible and convenient operation, system micromation, excellent portability, and the like, can be widely applied to the analysis and detection of cells and the early diagnosis of diseases, particularly the acquisition and detection of cancer cells and the early diagnosis of a cancer. The system also can be widely applied to food safety testing, environmental monitoring, medicaments/genes screening, and the like.
Description
Technical field
The invention belongs to the biological sample analysis technical field, be specifically related to the manipulation and the pick-up unit of cell in the early stage medical diagnosis on disease.
Background technology
Along with the raising of scientific-technical progress and people's living standard, people are more and more higher to the expectation requirement of health, and expectation requires treatment timely and effectively to particularly cancer energy early diagnosis of disease.By to biological sample, be to realize one of method to the disease early diagnosis as the analysis and the detection of protein, DNA, cell.Can help the doctor to find the state of an illness as early as possible, and take the treatment measure effectively, this has important effect and meaning to the general level of the health that improves people.Therefore, manipulation and the detection to the analyzing and testing of biological sample, particularly pair cell more and more causes scientific worker's attention.In order to realize quicker, more accurate, more stable cell detection analysis, people all seek a kind of can be with cell operation and integrated system and the device of measuring ability.
Existing cell operation and detection system, the system of building as Talanta the 74th phase 1621-1629 page or leaf in 2008 " analysis of interdigital microelectrode research cell solution electrochemical impedance spectroscopy " (Electrical impedance spectroscopy for detectionof bacterial cells in suspensions using interdigitated microelectrodes) is mainly by the microcavity body of one 25 μ L, and impedance detection microelectrode, impedance detector, cover glass and probe electrode are formed.Its microcavity body is to coat very thin layer of silica gel at the two ends of a base plate, forms at silica gel loam cake one cover glass again; The impedance detection microelectrode is golden array electrode, is arranged on the base plate in the microcavity body, and connects impedance detection microelectrode and impedance detector by probe electrode.The main shortcoming of this system is: can only the detection architecture impedance, can't realize the manipulation of cell, detectability is also very high simultaneously, under cell situation seldom, detection is less than useful signal, and disease is early stage the time, and the cell that indicates disease in the analytic sample usually seldom, therefore, this system does not reach the requirement of highly sensitive detection.And for example Appl.Phys. the 32nd phase 2814-2820 page or leaf " by microorganism concn in the dielectrophoresis impedance detecting electrode quantitative test solution " (Quantitativeestimation of biological cell concentration suspended in aqueous medium by usingdielectrophoretic impedance measurement method) the dielectrophoresis impedance detection system of building in 1999 is by sampling pump, the microcavity body, the dielectrophoresis chip, microscope, oscillograph and power supply are formed.The microcavity body that sampling pump is formed sample delivery to dielectrophoresis chip by pipeline, the dielectrophoresis chip is the gold electrode that comprises glass substrate and surface deposition thereof, can be directly behavior by cell on the microscopic examination dielectrophoresis chip; Oscillograph is connected on the circuit by electric wire, and impedance signal finally is sent to computing machine by data line.The main shortcoming of this system is: need use the large-scale instrument of microscope, oscillograph and so on, can not realize the integrated and microminiaturized of DEP analytic system; The circuit control of total system is unstable; Wherein, the gold electrode of dielectrophoresis chip is simultaneously also as impedance detection, makes the shared a pair of interdigital gold electrode of cell capture and impedance detection, and it is difficult on cell and the impedance high-sensitivity detection coordinate efficiently obtaining to catch.
Summary of the invention
The objective of the invention is deficiency, the micro-fluidic chip system of a kind of integrating cell operation and detection is provided, have highly integrated, efficient, high sensitivity and microminiaturized characteristics at existing cell operation and detection system.
Mechanism of the present invention: the present invention mainly is based on micro-fluidic chip dielectrophoresis (Dielectrophoresis, DEP) and the principle that detects of biological AC impedance, in inhomogeneous field, according to dielectric properties little, nano particle, to its technology of handling, this method of utilizing the polarization effect of particulate in inhomogeneous field to reach the manipulation particulate promptly is the dielectrophoresis technology.Dielectrophoresis and microfluidic analysis system combine and have formed micro-fluidic chip dielectrophoresis system, and demonstrate bigger advantage aspect cell operation.By can controlling the positive and negative of their suffered dielectrophoresises, thereby realize accurate manipulation little, nano particle to the change that applies signal frequency etc.; Aspect the detection and relevant information analysis of cell, based on biological AC impedance principle, the variation of electrical properties on micro-fluidic chip obtains corresponding electrical signal by cell and solution thereof, thereby reaches the purpose of check and analysis cell.The electric signal that obtains from cell can provide valuable information for researchs such as medical clinical diagnosis, food safety detection, environmental monitoring, medicine/genescreen and molecular structurology, cell biology and neuroscience and practical application.
The technical scheme that realizes the object of the invention is: the micro-fluidic chip system of a kind of integrating cell operation and detection mainly comprises AC signal power supply, microchip, integrated circuit control module and computing machine.The AC signal power supply is connected by the power connection of cable with the integrated circuit control module, microchip is connected with the chip interface of integrated circuit control module by its pin, the integrated circuit control module links to each other with computing machine by data line, has formed the complete stable integration dielectric electrophoresis (DEP) of a cover and has analyzed micro-system.
The AC signal power supply is adjustable AC signal generator, be electrically connected with the 220V city by power lead, its output signal voltage scope is 0～10V, frequency range is 100Hz～10MHz, be connected by the power connection of cable by signal output part with the integrated circuit control module, in order to integrated circuit control module and microchip are applied excitation signal, so that cell is caught and detected to cell operation cell electrode in the microchip and impedance detection cell electrode.
The integrated circuit control module is the external circuit control module, is integrated miniature control circuit board, and the length of its circuit board is 10～15cm, and wide is 8～10cm.The integrated circuit control module mainly comprises power connection, chip interface, analog multiplier, instrument amplifier, A/D data collecting card, electric capacity and resistance etc.Power connection is connected with the AC signal power supply by cable, in order to provide excitation signal to microchip and this unit.Electric capacity and resistance series connection back one end ground connection, the other end is connected with the output terminal of chip interface with after the input end of analog multiplier is in parallel again, is used for balance and stablizes the circuit of integrated circuit control unit.Chip interface is connected with microchip by the pin of microchip, with realization the sample in the microchip is applied signal, and sends detection signal to analog multiplier.Analog multiplier is accepted the detection signal by the microchip transmission, and after this signal is modulated into the differential signal that can amplify, send instrument amplifier to, after instrument amplifier carries out phase-locked amplification, be sent to the A/D data collecting card, the A/D data collecting card sends computing machine to after conversion of signals is become digital signal, carries out active data collection, demonstration and preservation.
Microchip is made up of the dimethyl silicone polymer that has pipeline (PDMS) cover plate and the glass substrate that deposits cell operation and detecting unit electrode.The duct length that has dimethyl silicone polymer (PDMS) cover plate of pipeline is 10mm～15mm, and its cross sectional shape is circular, and its external diameter is 150～300 μ m.One end of its pipeline is two fork arms, so that sample cell solution (as cancer cell or erythrocyte or leucocyte solution) after two fork arms inflow of this end, after pipeline flows out from the other end, flows into waste liquid pool again.Below having the dimethyl silicone polymer of pipeline (PDMS) cover plate, be connected with glass substrate by bonding, deposit cell operation cell electrode and impedance detection cell electrode on the glass substrate respectively.The cell operation cell electrode is in order to catch the cell in the sample cell solution in its pipeline.The impedance detection cell electrode detects the impedance of the cell after catching.Because along with constantly catching of cell, its cell concentration is along with increase, then the impedance detection signal also constantly strengthens, thereby the sensitivity that detects is improved.The cell operation cell electrode be array to electrode, totally 15～25 pairs, its material is that gold or titanium equistability can preferred metal.The length of each electrode is 50～70 μ m, and electrode sections is shaped as rectangle: its width is that 30～40 μ m, length are 50～70 μ m.The spacing of every pair of electrode is 30～50 μ m, and the spacing of adjacent two pairs of electrodes is 20～30 μ m.In order to the cell in the sample cell solution is handled (be that pair cell is caught, control such as separation and enrichment).The impedance detection cell electrode is the interdigital array electrode, and totally 25～50 pairs, its material is gold or titanium equistability energy preferred metal.The length of each electrode is 70～90 μ m, and electrode sections is shaped as rectangle: its width is 30 μ m～50 μ m, and length is 50 μ m～70 μ m.Spacing is 30～50 μ m between adjacent electrode, detects in order to the impedance of pair cell solution example.The cell operation cell electrode of intensive layout and impedance detection the cell electrode cell in the cell solution sample in the control area are effectively caught and impedance detection.The width of microchip is 14mm～50mm, and length is 25mm～50mm, is a micro-fluidic dielectrophoresis chip that integrates cell separation, enrichment, measuring ability, can realize in manipulated cell that pair cell carries out optionally check and analysis.
The digital signal of computing machine by converting to by the A/D data collecting card on the data line receiving integrate circuit control module, and show and the preservation data.
The cross sectional shape of the pipeline of the dimethyl silicone polymer that has pipeline (PDMS) cover plate described in the microchip of the present invention except that described circle, also has square or rectangular; The cell operation electrode at described glass substrate two ends and the cross sectional shape of impedance detecting electrode except that described rectangle, also have square or circular.
The present invention adopts technique scheme, mainly contains following effect:
1. microchip of the present invention separates cell operation and two unit of detection, and is arranged in simultaneously on the microchip, makes that can both work on maximum utility ground in the unit separately, has strengthened capture rate, has improved detection sensitivity;
2. adopt applying, amplify control and gathering of integrated circuit control module control signal, make entire circuit stability strengthen, operate more flexible;
3. integrated miniature control circuit board is adopted in external circuit control of the present invention, need not the large-scale observation instrument of microscope, oscillograph and so on again in the scheme, has realized the microminiaturization of DEP analytic system effectively, and portable better, easy to use.
The present invention can be widely used as the early diagnosis of the analyzing and testing and the disease of biological sample, be specially adapted to the aspects such as diagnosis with detection and early-stage cancer of catching of cellular elements biological study, cancer cell, also can be widely used as in the fields such as food safety detection, environmental monitoring, medicine/genescreen.
Description of drawings
Fig. 1 is a theory diagram of the present invention;
Fig. 2 is the structural representation of integrated circuit control module of the present invention;
Fig. 3 is the STRUCTURE DECOMPOSITION synoptic diagram of microchip among the present invention;
Fig. 4 is the quantitative test curve of present embodiment 1 cancer cell.
Among the figure: 1 computing machine, 2 AC signal power supplys, 3 integrated circuit control modules, 4 microchips, 5 power connections, 6 analog multipliers, 7 chip interfaces, 8 electric capacity, 9 resistance, 10 instrument amplifiers, 11 A/D data acquisition cards, 12 injection ports, 13 glass substrates, 14 have dimethyl silicone polymer (PDMS) cover plate of pipeline, 15 cell operation unit microelectrodes, 16 impedance detection unit microelectrodes.
Embodiment
Below in conjunction with embodiment, further specify the present invention.
Shown in Fig. 1～4, the micro-fluidic chip system of a kind of integrating cell operation and detection mainly comprises AC signal power supply 2, microchip 4, integrated circuit control module 3 and computing machine 1.AC signal power supply 2 connects by the power connection 5 of electric wire with integrated circuit control module 3, microchip 4 is connected with the chip interface 7 of integrated circuit control module by its pin, 3 of integrated circuit control modules link to each other with computing machine 1 by data line, have formed the complete stable integration dielectric electrophoresis (DEP) of a cover and have analyzed micro-system.
AC signal power supply 2 is adjustable AC signal generator, be electrically connected with the 220V city by power lead, its output signal voltage is 0～10V, frequency is 100Hz～10MHz, be connected with the power connection 5 of integrated circuit control module 3 by cable by signal output part, in order to apply excitation signal, so that cell is caught and detected to cell operation cell electrode 15 in the microchip 4 and impedance detection cell electrode 16.
Integrated circuit control module 3 is external circuit control modules, is integrated miniature control circuit board, and the length of its circuit board is 13cm, the wide 9cm of being.The integrated circuit control module mainly comprises power connection 5, chip interface 7, analog multiplier 6, instrument amplifier 10, A/D data collecting card 11, electric capacity 8 and resistance 9 etc.Power connection 5 connects with AC signal power supply 2 by electric wire, in order to provide excitation signal to microchip 4 and this unit.Electric capacity 8 and resistance 9 series connection back ground connection are used for balance and the circuit of stablizing integrated circuit control unit 3.Chip interface 7 is connected with microchip by the pin of microchip, with realization the sample on the microchip 4 is applied signal, and sends detection signal to analog multiplier 6.The detection signal that analog multiplier 6 is accepted by microchip 4 transmission, after it is modulated into the differential signal that can amplify with this signal, send instrument amplifier 10 to, after instrument amplifier is realized phase-locked amplification, be sent to A/D data collecting card 11, A/D data collecting card 11 sends computing machine 1 to after conversion of signals is become digital signal, realizes active data collection and preservation.
Microchip 4 is made up of the dimethyl silicone polymer that has pipeline (PDMS) cover plate 14 and the glass substrate 13 that deposits cell operation and detecting unit electrode 15,16.The duct length that has dimethyl silicone polymer (PDMS) cover plate 14 of pipeline is 12mm, and its cross section is circular, and its external diameter is 200 μ m.One end of its pipeline is two fork arms 12, so that tested cell solution (as cancer cell solution) sample liquid flows into waste liquid pool through pipeline from the other end after two fork arms inflow of this end.Be connected with glass substrate 13 below by bonding having the dimethyl silicone polymer of pipeline (PDMS) cover plate 14, on glass substrate 13, deposit cell operation cell electrode 15 and impedance detection cell electrode 16 respectively.Cell operation cell electrode 15 is in order to catch the cell in the tested cell solution sample in cover plate 14 pipelines, and the impedance of the cell after 16 pairs of impedance detection cell electrode are caught detects.Because along with constantly catching of cell, its cell concentration is along with increase, then the impedance detection signal also constantly strengthens, thereby the sensitivity that detects is improved.Cell operation cell electrode 15 is that the array titanium is little to electrode, and deposition one deck titanium on glass substrate, and then deposited gold earlier forms electrode at last, helps the stable of electrode like this, totally 20 pairs at electrode.The length of each electrode is 60 μ m, and electrode sections is shaped as rectangle, and its width is 35 μ m, length is 60 μ m, and the spacing of every pair of electrode is 40 μ m, and the spacing of adjacent two pairs of electrodes is 25 μ m, handle in order to pair cell, promptly pair cell catch, control such as separation and enrichment.Impedance detection unit 16 is an interdigital titanium tiny array electrode, and deposition one deck titanium on glass substrate, and then deposited gold earlier forms electrode at last, helps the stable of electrode like this, totally 30 pairs at electrode.The length of each electrode is 80 μ m, and electrode sections is shaped as rectangle, and its width is 40 μ m, and length is 60 μ m, and spacing is 40 μ m between adjacent electrode.The cell operation cell electrode of intensive layout and impedance detection cell electrode 15,16 cell in the cell solution sample in the control area are effectively caught and impedance detection.The width of microchip 4 is 30mm, and length is 40mm, is a micro-fluidic dielectrophoresis chip that integrates cell separation, enrichment, measuring ability, can realize in manipulated cell that pair cell carries out optionally check and analysis.
The digital signal of computing machine 1 by converting to by A/D data collecting card 11 on the data line receiving integrate circuit control module, and show and the preservation data.Total system has realized erythrocyte is separated with effective DEP of hepatoma carcinoma cell and catches, and the capture rate of hepatoma carcinoma cell can reach more than 80%, and the data of impedance detection can be carried out quantitative effectively to the hepatoma carcinoma cell of catching.
The micro-fluidic chip system of a kind of integrating cell operation and detection, with embodiment 1, wherein: integrated circuit control module 3 is external circuit control modules, is integrated miniature control circuit board, the length of its circuit board is 10cm, the wide 8cm of being.Dimethyl silicone polymer (PDMS) the cover plate duct length that has pipeline is 10mm, and its cross sectional shape is circular, and its external diameter is 150 μ m.Actuation unit electrode 15 is that the array titanium is little to electrode, totally 15 pairs.The length of each electrode is 50 μ m, and electrode sections is shaped as rectangle, and its width is 30 μ m, and length is 50 μ m, and the spacing of every pair of electrode is 30 μ m, and the spacing of adjacent two pairs of electrodes is 20 μ m.Impedance detection cell electrode 16 is an interdigital titanium tiny array electrode, totally 25 pairs.The length of each electrode is 70 μ m, and electrode sections is shaped as rectangle, and its width is 30 μ m, and length is 50 μ m, and spacing is 30 μ m between adjacent electrode.The width of microchip is 14mm, and length is 25mm.
The micro-fluidic chip system of a kind of integrating cell operation and detection, with embodiment 1, wherein: integrated circuit control module 3 is external circuit control modules, is integrated miniature control circuit board, the length of its circuit board is 15cm, the wide 10cm of being.Dimethyl silicone polymer (PDMS) the cover plate duct length that has pipeline is 10mm, and its cross sectional shape is circular, and its external diameter is 300 μ m.Actuation unit electrode 15 is that the array titanium is little to electrode, totally 25 pairs.The length of each electrode is 70 μ m, and electrode sections is shaped as rectangle, and its width is 40 μ m, and length is 70 μ m, and the spacing of every pair of electrode is 50 μ m, and the spacing of adjacent two pairs of electrodes is 30 μ m.Impedance detection cell electrode 16 is an interdigital titanium tiny array electrode, totally 50 pairs.The length of each electrode is 90 μ m, and electrode sections is shaped as rectangle, and its width is 50 μ m, and length is 70 μ m, and spacing is 50 μ m between adjacent electrode.The width of microchip is 40mm, and length is 50mm.
Experimental result
With a kind of integrating cell operation of embodiment 1 and the micro-fluidic chip system of detection, the sample cancer cell solution for preparing is imported in the injection port 12 of microchip, connected AC signal power supply 2, detect, record the quantitative test curve of cancer cell, as shown in Figure 4.
Know from above-mentioned experiment: the micro-fluidic chip system of integrating cell operation of the present invention and detection, can carry out effective DEP to the cancer cell in the cancer cell solution example and catch, and can carry out quantitative test to cancer cell by impedance detection.In cancer cell concentration is 10
4Under the above situation, the curvilinear equation between its resistance value Z and the cell concentration C is Z=-0.018C+9895.9, R
2=0.9962, illustrate that its linear dependence is better, can comparatively effectively be used for the quantitative test of cancer cell.The cell operation cell electrode can utilize DEP that the cancer cell solution example of low concentration is concentrated, carry out the resistance value that records after DEP catches by the typical curve of Fig. 4 and to cancer cell, cancer cell concentration after quantitatively going out DEP and catching is more than 2 times of former cancer cell solution example, and DEP can reach more than 80% the cancer cell capture rate.
Claims (4)
1. the micro-fluidic chip system of integrating cell operation and detection, mainly comprise computing machine (1), AC signal power supply (2), it is characterized in that also having integrated circuit control module (3) and microchip (4), AC signal power supply (2) is connected with the power connection (5) of integrated circuit control module (3) by cable, microchip (4) is connected with the chip interface (7) of integrated circuit control module by its pin, integrated circuit control module (3) links to each other with computing machine (1) by data line, AC signal power supply (2) is adjustable AC signal generator, be electrically connected with the 220V city by power lead, its output signal voltage scope is 0～10V, frequency range is 100Hz～10MHz, be connected with the power connection (5) of integrated circuit control module (3) by cable by signal output part, integrated circuit control module (3) is the external circuit control module, be integrated miniature control circuit board, the length of its circuit board is 10～15cm, wide is 8～10cm, integrated circuit control module (3) mainly comprises power connection (5), chip interface (7), analog multiplier (6), instrument amplifier (10), A/D data collecting card (11), electric capacity (8) and resistance (9), power connection (5) is connected with AC signal power supply (2) by cable, electric capacity (8) and resistance (9) series connection back one end ground connection, the other end is with after the input end of analog multiplier (6) is in parallel, output terminal with chip interface (7) is connected again, chip interface (7) is connected with microchip (4) by the pin of microchip (4), analog multiplier (6) is accepted the detection signal by microchip (4) transmission, and after this signal is modulated into the differential signal that can amplify, send instrument amplifier (10) to, after instrument amplifier (10) carries out phase-locked amplification, be sent to A/D data collecting card (11), after A/D data collecting card (11) becomes digital signal with conversion of signals, send computing machine (1) to, microchip (4) is by the dimethyl silicone polymer cover plate (14) that has pipeline and deposit cell operation and detecting unit electrode (15,16) glass substrate (13) is formed, the duct length that has the dimethyl silicone polymer cover plate (14) of pipeline is 10mm～15mm, its cross sectional shape is circular, its external diameter is 150～300 μ m, one end of its pipeline is two fork arms, below having the dimethyl silicone polymer cover plate (14) of pipeline, be connected with by bonding and deposit glass substrate (13), on glass substrate (13), deposit cell operation cell electrode (15) and impedance detection cell electrode (16) respectively, cell operation cell electrode (15) is that array is to electrode, totally 15～25 pairs, its material is a titanium, the length of each electrode is 50～70 μ m, electrode sections is shaped as rectangle, its width is 30～40 μ m, length is 50～70 μ m, the spacing of every pair of electrode is 30～50 μ m, the spacing of adjacent two pairs of electrodes is 20～30 μ m, impedance detection cell electrode (16) is the interdigital array electrode, totally 25～50 pairs, its material is a titanium, the length of each electrode is 70～90 μ m, electrode sections is shaped as rectangle, its width is 30 μ m～50 μ m, length is 50 μ m～70 μ m, spacing is 30～50 μ m between adjacent electrode, the width of microchip (4) is 14mm～50mm, length is 25mm～50mm, the cross sectional shape of the pipeline of the dimethyl silicone polymer cover plate (14) that has pipeline described in the microchip of the present invention, except that described circle, also has square or rectangular, the cell operation electrode (15) at described glass substrate (13) two ends and the cross sectional shape of impedance detecting electrode (16), except that described rectangle, also has square or circular.
2. according to the micro-fluidic chip system of described integrating cell operation of claim 1 and detection, it is characterized in that integrated circuit control module (3) is the external circuit control module, be integrated miniature control circuit board, the length of its circuit board is 13cm, wide is 9cm, dimethyl silicone polymer cover plate (14) duct length that has pipeline is 12mm, its sectional area is the circle rectangle, its external diameter is 200 μ m, and actuation unit electrode (15) is that the array titanium is little to electrode, totally 20 pairs, the length of each electrode is 60 μ m, electrode sections is shaped as rectangle, and its width is 35 μ m, and length is 60 μ m, the spacing of every pair of electrode is 40 μ m, the spacing of adjacent two pairs of electrodes is 25 μ m, and impedance detection cell electrode (16) is an interdigital titanium tiny array electrode, totally 30 pairs, the length of each electrode is 80 μ m, electrode sections is shaped as rectangle, and its width is 40 μ m, and length is 60 μ m, spacing is 40 μ m between adjacent electrode, and the width of microchip (4) is 30mm, length is 40mm.
3. according to the micro-fluidic chip system of described integrating cell operation of claim 1 and detection, it is characterized in that integrated circuit control module (3) is the external circuit control module, be integrated miniature control circuit board, the length of its circuit board is 10cm, wide is 8cm, dimethyl silicone polymer cover plate (14) duct length that has pipeline is 10mm, its cross sectional shape is circular, its external diameter is 150 μ m, and actuation unit electrode (15) is that the array titanium is little to electrode, totally 15 pairs, the length of each electrode is 50 μ m, electrode sections is shaped as rectangle, and its width is 30 μ m, and length is 50 μ m, the spacing of every pair of electrode is 30 μ m, the spacing of adjacent two pairs of electrodes is 20 μ m, and impedance detection cell electrode (16) is an interdigital titanium tiny array electrode, totally 25 pairs, the length of each electrode is 70 μ m, electrode sections is shaped as rectangle, and its width is 30 μ m, and length is 50 μ m, spacing is 30 μ m between adjacent electrode, and the width of microchip (4) is 14mm, length is 25mm.
4. according to the micro-fluidic chip system of described integrating cell operation of claim 1 and detection, it is characterized in that integrated circuit control module (3) is the external circuit control module, be integrated miniature control circuit board, the length of its circuit board is 15cm, wide is 10cm, dimethyl silicone polymer cover plate (14) duct length that has pipeline is 10mm, its sectional area is a rectangle, width is 150 μ m, highly be 30 μ m, actuation unit electrode (15) is that the array titanium is little to electrode, totally 25 pairs, the length of each electrode is 70 μ m, electrode sections is shaped as square, and the length of side is 40 μ m, and the spacing of every pair of electrode is 40 μ m, the spacing of adjacent two pairs of electrodes is 25 μ m, impedance detection cell electrode (16) is an interdigital titanium tiny array electrode, and totally 50 pairs, the length of each electrode is 90 μ m, electrode sections is shaped as square, its length of side is 40 μ m, and spacing is 40 μ m between adjacent electrode, and the width of microchip (4) is 40mm, length is 50mm.
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