CN112834588A - Micro-fluidic chip for virus electrical impedance real-time monitoring - Google Patents
Micro-fluidic chip for virus electrical impedance real-time monitoring Download PDFInfo
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- 238000012544 monitoring process Methods 0.000 title claims abstract description 21
- 241000700605 Viruses Species 0.000 title abstract description 34
- 238000001514 detection method Methods 0.000 claims abstract description 50
- 239000007788 liquid Substances 0.000 claims abstract description 27
- 239000000758 substrate Substances 0.000 claims abstract description 15
- 230000003612 virological effect Effects 0.000 claims description 10
- 239000000565 sealant Substances 0.000 claims description 5
- 239000002184 metal Substances 0.000 claims description 4
- 239000000853 adhesive Substances 0.000 claims description 3
- 230000001070 adhesive effect Effects 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 description 18
- 241000711573 Coronaviridae Species 0.000 description 12
- 238000000034 method Methods 0.000 description 12
- 102000039446 nucleic acids Human genes 0.000 description 6
- 108020004707 nucleic acids Proteins 0.000 description 6
- 150000007523 nucleic acids Chemical class 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 238000011160 research Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 239000011230 binding agent Substances 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 239000004205 dimethyl polysiloxane Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000001566 impedance spectroscopy Methods 0.000 description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 2
- 238000011897 real-time detection Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 102100031673 Corneodesmosin Human genes 0.000 description 1
- 101710139375 Corneodesmosin Proteins 0.000 description 1
- -1 Polydimethylsiloxane Polymers 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000004720 dielectrophoresis Methods 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000001453 impedance spectrum Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 102000006240 membrane receptors Human genes 0.000 description 1
- 108020004084 membrane receptors Proteins 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000002174 soft lithography Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000010408 sweeping Methods 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
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- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The invention discloses a micro-fluidic chip for virus electrical impedance real-time monitoring, which consists of a chip substrate, a shielding electrode ring, a sensor array and a shielding electrode, wherein the upper surface of the chip substrate comprises a liquid storage tank, a shielding electrode ring groove and a round electrode groove, the shielding electrode ring groove and the round electrode groove are embedded in the liquid storage tank, the lower surface of the chip substrate comprises a strip-shaped electrode groove and a shielding electrode groove, and the sensor array is formed by connecting a round electrode and a strip-shaped electrode. And the six liquid storage tanks are arranged, so that various detection liquids can be injected simultaneously to detect the virus, and the reliability of virus detection is improved.
Description
Technical Field
The invention relates to a micro-fluidic chip, in particular to a micro-fluidic chip for virus electrical impedance real-time monitoring, and belongs to the technical field of micro-fluidic chips.
Background
The detection methods of the coronavirus mainly comprise a nucleic acid detection method and an antibody detection method. The nucleic acid detection process comprises a plurality of steps of sample treatment, nucleic acid extraction, PCR detection and the like, and the average detection time is long and needs 2-3 hours. The method is used for directly detecting virus nucleic acid in a collected specimen, has strong specificity and relatively high sensitivity, is a current main detection means, detects the antibody level in human blood by antibody detection, has a detection window period, can be used for auxiliary diagnosis of negative cases of nucleic acid detection, and can also be used for investigation and screening of cases, but cannot replace a nucleic acid detection method. Since the major determinant of coronavirus infectivity is the S protein, which binds to membrane receptors on host cells, mediating fusion of the virus and cell membranes, it is contemplated that viruses can be detected by detecting cells infected with coronavirus.
The Bio Impedance Spectroscopy (BIS) technology realizes real-time detection of coronavirus by detecting the relation between the dielectric property of a sample and an alternating current electric field excitation signal. The detection method has the advantages of no mark, simple operation, high detection speed and the like. The BIS technique obtains an impedance spectrum of a sample by sweeping a frequency of the sample to be detected, thereby analyzing components of the sample and detecting whether the sample contains cells infected with coronavirus. However, BIS presents two problems in the detection of coronaviruses: firstly, the concentration of infected coronavirus cells in a sample affects the measurement result of BIS, thereby affecting the analysis of sample components; secondly, the coronavirus is hosted in the host cell, and the ultrahigh frequency excitation signal is required to penetrate through the cell membrane to detect whether the coronavirus is parasitized by the virus, however, the ultrahigh frequency BIS has extremely high requirements on a detection chip and a peripheral circuit. Aiming at the two problems, a method using ultra-high frequency impedance spectroscopy (uf-BIS) is provided to realize real-time detection of the new coronavirus. The method is characterized in that the cells possibly infected with coronavirus are enriched by dielectrophoresis, and then the cells obtained by enrichment are subjected to uf-BIS detection, so that real-time coronavirus detection is realized.
Disclosure of Invention
The invention aims to provide a micro-fluidic chip for real-time monitoring of viral electrical impedance, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a micro-fluidic chip for virus electrical impedance real-time supervision, micro-fluidic chip comprises chip base member, shielding electrode circle, sensor array and shielding electrode, the upper surface of chip base member contains reservoir, shielding electrode circle groove and circle electrode groove, shielding electrode circle groove and circle electrode inslot are in the reservoir, the lower surface of chip base member contains strip electrode groove and shielding electrode groove, the sensor array is formed by round electrode and strip electrode link to each other.
As a preferable technical scheme of the invention, the number of the liquid storage tanks is six, the six groups of liquid storage tanks are in the same shape, and the six groups of liquid storage tanks are uniformly distributed on the upper surface of the chip matrix.
As a preferred technical solution of the present invention, the number of the shielding electrode rings is six, and the six groups of the shielding electrode rings are completely the same, and the six groups of the shielding electrode rings are uniformly distributed inside the shielding electrode ring groove of the corresponding liquid storage tank.
As a preferred technical scheme of the invention, the round electrode and the strip electrode are respectively connected with the corresponding round electrode groove and the strip electrode groove.
As a preferred technical solution of the present invention, two of the strip-shaped electrodes located at both sides are rich electrodes, and one of the strip-shaped electrodes located in the middle is a detection electrode.
As a preferable technical scheme of the invention, the diameter of the round electrode and the diameter of the strip electrode are both 10 μm.
As a preferred embodiment of the present invention, the shielding electrode is connected to the shielding electrode groove by an adhesive and a sealant, the shielding electrode and the shielding electrode ring are connected to each other inside the chip substrate, and the shielding electrode is grounded inside the chip substrate.
As a preferable technical scheme of the invention, the detection electrode is connected with an external impedance analyzer through a metal wire deposited on the surface of the substrate.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention realizes the enrichment and real-time monitoring of virus infected cells on one chip, shortens the original long and multi-step virus detection process to several minutes, and improves the detection efficiency by a single step.
2. The invention monitors the cells infected by the virus in real time, avoids the death of the virus or the cells in the process of transferring or processing the cells by the traditional sampling method, and greatly improves the accuracy of virus detection.
3. The invention has six liquid storage tanks, can inject a plurality of detection liquids to detect the virus at the same time, and increases the reliability of virus detection.
4. The detection electrode is directly connected with an external impedance analyzer, can observe the state of virus-infected cells in real time, and has certain scientific research value.
5. The invention uses ultra-high frequency detection, and the shielding electrodes are distributed around the enrichment electrode and the detection electrode, thereby improving the accuracy of detection.
Drawings
FIG. 1 is a perspective view of a real-time virus monitoring microfluidic chip of the present invention, FIG. 1;
FIG. 2 is a perspective view of a real-time virus monitoring microfluidic chip according to the present invention, FIG. 2;
FIG. 3 is a perspective view of a core substrate portion of a microfluidic chip for real-time monitoring of hand virus according to the present invention;
FIG. 4 is a perspective view of a core substrate part of a microfluidic chip for real-time monitoring of hand virus according to the present invention, shown in FIG. 2;
FIG. 5 is a perspective view of a sensor array part of a micro-fluidic chip for real-time monitoring of hand viruses according to the present invention.
In the figure: 1. a chip substrate; 2. a shielding electrode ring; 3. an array of sensors; 4. a shield electrode; 5. a liquid storage tank; 6. a shield electrode ring slot; 7. a circular electrode groove; 8. a strip-shaped electrode groove; 9. a shield electrode tank; 10. a circular electrode; 11. a strip electrode; 12. an enrichment electrode; 13. and a detection electrode.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, the present invention provides a technical solution of a microfluidic chip for real-time monitoring of viral electrical impedance:
as shown in fig. 1 to 5, the microfluidic chip is composed of a chip substrate 1, a shielding electrode ring 2, a sensor array 3 and a shielding electrode 4, wherein the upper surface of the chip substrate 1 includes a liquid storage tank 5, a shielding electrode ring tank 6 and a circular electrode tank 7, the shielding electrode ring tank 6 and the circular electrode tank 7 are embedded in the liquid storage tank 5, the lower surface of the chip substrate 1 includes a strip-shaped electrode tank 8 and a shielding electrode tank 9, and the sensor array 3 is formed by connecting a circular electrode 10 and a strip-shaped electrode 11.
According to the figure 1 and figure 5, the number of the liquid storage tanks 5 is six, the six groups of liquid storage tanks 5 are in the same shape, the six groups of liquid storage tanks 5 are uniformly distributed on the upper surface of the chip base body 1, the number of the shielding electrode rings 2 is six, the six groups of shielding electrode rings 2 are completely the same, the six groups of shielding electrode rings 2 are uniformly distributed in the shielding electrode ring grooves 6 of the corresponding liquid storage tanks 5, a plurality of detection liquids can be injected simultaneously for virus detection, the virus detection reliability is increased, the circular electrodes 10 and the strip electrodes 11 are respectively connected with the corresponding circular electrode grooves 7 and strip electrode grooves 8, the two strip electrodes 11 positioned at two sides are enrichment electrodes 12, one strip electrode 11 positioned in the middle is a detection electrode 13, the diameter of the circular electrode 10 and the diameter of the strip electrode 11 are both 10 μm, the shielding electrode 4 is connected with the shielding electrode grooves 9 through an adhesive and a sealant, shielding electrode 4 links to each other at the inside of chip base member 1 with shielding electrode circle 2, and shielding electrode 4 is at the inside ground connection of chip base member 1, and detecting electrode 13 passes through the sedimentary metal wire of base plate surface and links to each other with external impedance analysis appearance, has improved the rate of accuracy that detects, can observe the state by virus infection cell in real time simultaneously, possess certain scientific research value, and the practicality is strong.
When the micro-fluidic chip is used specifically, the micro-fluidic chip for virus electrical impedance real-time monitoring is manufactured by combining Polydimethylsiloxane (PDMS) with a soft lithography method to form a chip substrate 1, then a round electrode 10 is connected with a round electrode groove 7 through a binder and a sealant in sequence, a shielding electrode 4 is connected with a shielding electrode groove 9, an enrichment electrode 12 and a detection electrode 13 are connected to a strip electrode groove 8, six groups of shielding electrode rings 2 are uniformly distributed inside a shielding electrode ring groove 6 corresponding to a liquid storage tank 5 through the binder and the sealant, the detection electrode 13 is connected with an external impedance analyzer through a metal lead deposited on the surface of the chip substrate 1, and detection liquid is injected to detect viruses.
In conclusion, the invention realizes the enrichment and real-time monitoring of virus infected cells, shortens the originally long and time-consuming multi-step virus detection process to several minutes, and improves the detection efficiency by a single step; the cell infected by the virus is monitored in real time, so that the death of the virus or the cell in the process of transferring or treating the cell by the traditional sampling method is avoided, and the accuracy of virus detection is greatly improved; the invention has six liquid storage tanks, can inject multiple detection liquids into the liquid storage tanks at the same time to carry out virus detection, and increases the reliability of virus detection; the detection electrode is directly connected with an external impedance analyzer, so that the state of the virus-infected cell can be observed in real time, and certain scientific research value is achieved; and by adopting ultrahigh frequency detection, shielding electrodes are distributed around the enrichment electrode and the detection electrode, so that the detection accuracy is improved.
In the description of the present invention, it is to be understood that the indicated orientations or positional relationships are based on the orientations or positional relationships shown in the drawings and are only for convenience in describing the present invention and simplifying the description, but are not intended to indicate or imply that the indicated devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be construed as limiting the present invention.
In the present invention, unless otherwise explicitly specified or limited, for example, it may be fixedly attached, detachably attached, or integrated; can be mechanically or electrically connected; the terms may be directly connected or indirectly connected through an intermediate, and may be communication between two elements or interaction relationship between two elements, unless otherwise specifically limited, and the specific meaning of the terms in the present invention will be understood by those skilled in the art according to specific situations.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The utility model provides a micro-fluidic chip for viral electrical impedance real-time supervision, its characterized in that, micro-fluidic chip comprises chip base member (1), shielding electrode circle (2), sensor array (3) and shielding electrode (4), the upper surface of chip base member (1) contains reservoir (5), shielding electrode circle groove (6) and round electrode groove (7), embedded in reservoir (5) in shielding electrode circle groove (6) and round electrode groove (7), the lower surface of chip base member (1) contains strip electrode groove (8) and shielding electrode groove (9), sensor array (3) are formed by round electrode (10) and strip electrode (11) link to each other.
2. The microfluidic chip for real-time monitoring of viral electrical impedance according to claim 1, wherein: the number of the liquid storage tanks (5) is six, the six liquid storage tanks (5) are identical in shape, and the six liquid storage tanks (5) are uniformly distributed on the upper surface of the chip base body (1).
3. The microfluidic chip for real-time monitoring of viral electrical impedance according to claim 1, wherein: the number of the shielding electrode rings (2) is six, the six groups of the shielding electrode rings (2) are completely the same, and the six groups of the shielding electrode rings (2) are uniformly distributed in the shielding electrode ring grooves (6) of the corresponding liquid storage tanks (5).
4. The microfluidic chip for real-time monitoring of viral electrical impedance according to claim 1, wherein: the round electrode (10) and the strip electrode (11) are respectively connected with the corresponding round electrode groove (7) and the strip electrode groove (8).
5. The microfluidic chip for real-time monitoring of viral electrical impedance according to claim 1, wherein: the two strip-shaped electrodes (11) positioned at two sides are enrichment electrodes (12), and the strip-shaped electrode (11) positioned in the middle is a detection electrode (13).
6. The microfluidic chip for real-time monitoring of viral electrical impedance according to claim 1, wherein: the diameter of the round electrode (10) and the diameter of the strip-shaped electrode (11) are both 10 micrometers.
7. The microfluidic chip for real-time monitoring of viral electrical impedance according to claim 1, wherein: the shielding electrode (4) is connected with the shielding electrode groove (9) through an adhesive and a sealant, the shielding electrode (4) is connected with the shielding electrode ring (2) in the chip base body (1), and the shielding electrode (4) is grounded in the chip base body (1).
8. The microfluidic chip for real-time monitoring of viral electrical impedance according to claim 5, wherein: and the detection electrode (13) is connected with an external impedance analyzer through a metal wire deposited on the surface of the substrate.
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| CN202110208269.9A CN112834588A (en) | 2021-02-24 | 2021-02-24 | Micro-fluidic chip for virus electrical impedance real-time monitoring |
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| CN202110208269.9A CN112834588A (en) | 2021-02-24 | 2021-02-24 | Micro-fluidic chip for virus electrical impedance real-time monitoring |
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|---|---|---|---|---|
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| CN215179869U (en) * | 2021-02-24 | 2021-12-14 | 江苏济纶医工智能科技有限公司 | Micro-fluidic chip for virus electrical impedance real-time monitoring |
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2021
- 2021-02-24 CN CN202110208269.9A patent/CN112834588A/en active Pending
Patent Citations (6)
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| WO2018234590A1 (en) * | 2017-06-23 | 2018-12-27 | Cellix Limited | A microfluidic apparatus for separation of particulates in a fluid |
| CN109865539A (en) * | 2017-12-04 | 2019-06-11 | 上海新微技术研发中心有限公司 | Micro-fluidic chip of integrated electrochemical sensor and manufacturing method and application thereof |
| CN108387488A (en) * | 2018-02-28 | 2018-08-10 | 北京怡天佳瑞科技有限公司 | Particulate matter detection means and detection method |
| CN110935493A (en) * | 2019-11-28 | 2020-03-31 | 北京乐普医疗科技有限责任公司 | Micro-fluidic chip and detection method for detection by using same |
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