CN215103281U - Cell culture freezes and deposits water bath box - Google Patents

Cell culture freezes and deposits water bath box Download PDF

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Publication number
CN215103281U
CN215103281U CN202121596867.XU CN202121596867U CN215103281U CN 215103281 U CN215103281 U CN 215103281U CN 202121596867 U CN202121596867 U CN 202121596867U CN 215103281 U CN215103281 U CN 215103281U
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China
Prior art keywords
box body
rod
cell culture
water bath
fixed plate
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CN202121596867.XU
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Chinese (zh)
Inventor
张露文
邵明龙
刘青
柴瑞
王琪
李文强
吕路线
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Second Affiliated Hospital of Xinxiang Medical University
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Second Affiliated Hospital of Xinxiang Medical University
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Abstract

The utility model discloses a cell culture freezes and deposits water bath box, include the box body, rock subassembly, sealed lid and fixed subassembly, rock the subassembly and locate on the box body, sealed lid is located and is rocked on the subassembly, fixed subassembly is located and is sealed covered, it includes gag lever post, backup pad, connecting rod, fixed plate, directional bar, motor, eccentric wheel, spacing frame and slide bar to rock the subassembly, the box body is located to the gag lever post, the backup pad slides and cup joints on locating the gag lever post, the connecting rod is located in the backup pad, the fixed plate is located on the connecting rod, the slide bar is located on the fixed plate and slides and run through the box body and extend to outside the box body, spacing frame is located on the slide bar, the motor is located on the box body, the eccentric wheel is located on the motor and rotatory slip is located in the spacing frame. The utility model relates to a cell culture technical field specifically provides a degree of automation is high, and work efficiency is high, can avoid the cell culture that the cell received the pollution to freeze and deposit the water bath box.

Description

Cell culture freezes and deposits water bath box
Technical Field
The utility model relates to a cell culture technical field specifically indicates a cell culture freezes water bath box.
Background
The cell cryopreservation is one of the main methods for cell preservation, the cryopreservation technology is utilized to place the cells in liquid nitrogen at the temperature of-196 ℃ for low-temperature preservation, the cells can be temporarily separated from the growth state and can be preserved according to the cell characteristics, the purpose of cell cryopreservation is to resuscitate the cells for experiments when needed, a water bath box is a common tool for resuscitating the cells after cell cryopreservation, the existing water bath box cannot resuscitate multiple groups of cells simultaneously, the working efficiency is low, and the cells are easily polluted in the process of resuscitating the cells.
SUMMERY OF THE UTILITY MODEL
To the above-mentioned condition, for overcoming prior art's defect, the utility model provides an degree of automation is high, and work efficiency is high, can avoid the cell culture that the cell received the pollution to freeze the water bath box.
The utility model adopts the following technical scheme: the utility model relates to a cell culture freezing water bath box, which comprises a box body, a shaking component, a sealing cover and a fixing component, wherein the shaking component is arranged on the box body, the sealing cover is arranged on the shaking component, the fixing component is arranged on the sealing cover, the shaking component comprises a limiting rod, a supporting plate, a connecting rod, a fixed plate, a directional rod, a motor, an eccentric wheel, a limiting frame and a sliding rod, the limiting rod is arranged in the box body, the supporting plate is sleeved on the limiting rod in a sliding way, the connecting rod is arranged on the supporting plate, the fixed plate is arranged on the connecting rod, the sliding rod is arranged on the fixed plate and extends out of the box body through the box body in a sliding way, the limiting frame is arranged on the sliding rod, the motor is arranged on the box body, the eccentric wheel is arranged on the motor and is arranged in the limiting frame in a rotating and sliding way, a sleeve is arranged in the box body, the directional rod is arranged on the fixed plate and is arranged in the sleeve in a sliding way, the fixed plate is provided with a vertical rod, and the sealing cover is sleeved on the vertical rod in a sliding manner.
Further, fixed subassembly includes pull rod, pull ring, spring and limiting plate, sealed covering is equipped with the slip chamber, be equipped with the draw-in groove on the montant, the pull rod slides and runs through in the slip chamber extends to the draw-in groove, the limiting plate is located on the pull rod and is slided and locate the slip intracavity, the spring cup joints on locating the pull rod, the both ends of spring are located respectively on limiting plate and the slip intracavity wall, the pull ring is located on the pull rod.
Furthermore, the box body is provided with a water inlet pipe.
Furthermore, a water outlet pipe is arranged on the box body, and an electromagnetic valve is arranged on the water outlet pipe.
Furthermore, a top cover is hinged on the box body.
Furthermore, a heating element and a temperature sensor are arranged in the box body.
Furthermore, the fixing plate is provided with through holes, the supporting plate is provided with fixing seats, the through holes are provided with a plurality of groups, and the number of the fixing seats is equal to that of the through holes and corresponds to that of the through holes one by one.
Adopt above-mentioned structure the utility model discloses the beneficial effect who gains as follows: the cell thawing device is provided with the shaking assembly, manual shaking can be replaced by the shaking assembly, frozen liquid in the frozen tube can be thawed quickly, cell thawing is accelerated, multiple groups of cells can be thawed simultaneously, and the cell thawing quantity and the working efficiency are effectively improved; the freezing tube can be blocked through the sealing cover, so that cell pollution caused by water bath liquid entering the freezing tube in the shaking process is avoided; this scheme is equipped with fixed subassembly, can fix sealed lid through fixed subassembly, avoids also rocking thereupon freezing the sealed lid of in-process that deposits the pipe and rock.
Drawings
The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic view of the overall structure of a water bath box for cell culture freezing according to the present invention;
FIG. 2 is an enlarged view of part A of the overall structural schematic diagram of the water bath box for cell culture freezing according to the present invention;
figure 3 is the utility model discloses a cell culture freezes deposits water bath box in eccentric wheel and spacing frame's structural schematic diagram.
The automatic water feeding device comprises a box body 1, a box body 2, a shaking component 3, a sealing cover 4, a fixing component 5, a limiting rod 6, a supporting plate 7, a connecting rod 8, a fixing plate 9, a directional rod 10, a motor 11, an eccentric wheel 12, a limiting frame 13, a sliding rod 14, a pull rod 15, a pull ring 16, a spring 17, a limiting plate 18, a clamping groove 19, a water inlet pipe 20, a water outlet pipe 21, an electromagnetic valve 22, a top cover 23, a heating element 24 and a temperature sensor.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments; based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
As shown in figures 1-3, the utility model relates to a cell culture freezing water bath box, which comprises a box body 1, a shaking component 2, a sealing cover 3 and a fixing component 4, wherein the shaking component 2 is arranged on the box body 1, the sealing cover 3 is arranged on the shaking component 2, the fixing component 4 is arranged on the sealing cover 3, the shaking component 2 comprises a limit rod 5, a support plate 6, a connecting rod 7, a fixed plate 8, a directional rod 9, a motor 10, an eccentric wheel 11, a limit frame 12 and a sliding rod 13, the limit rod 5 is arranged in the box body 1, the support plate 6 is slidably sleeved on the limit rod 5, the connecting rod 7 is arranged on the support plate 6, the fixed plate 8 is arranged on the connecting rod 7, the sliding rod 13 is arranged on the fixed plate 8 and slidably penetrates through the box body 1 and extends out of the box body 1, the limit frame 12 is arranged on the sliding rod 13, the motor 10 is arranged on the box body 1, the eccentric wheel 11 is arranged on the motor 10 and is rotatably and slidably arranged in the limiting frame 12, a sleeve is arranged in the box body 1, the directional rod 9 is arranged on the fixed plate 8 and is slidably arranged in the sleeve, a vertical rod is arranged on the fixed plate 8, and the sealing cover 3 is slidably sleeved on the vertical rod.
The fixing component 4 comprises a pull rod 14, a pull ring 15, a spring 16 and a limit plate 17, a sliding cavity is arranged on the sealing cover 3, a clamping groove 18 is arranged on the vertical rod, the pull rod 14 penetrates through the sliding cavity in a sliding mode and extends into the clamping groove 18, the limit plate 17 is arranged on the pull rod 14 and is arranged in the sliding cavity in a sliding mode, the spring 16 is sleeved on the pull rod 14, two ends of the spring 16 are respectively arranged on the limit plate 17 and the inner wall of the sliding cavity, and the pull ring 15 is arranged on the pull rod 14; the box body 1 is provided with a water inlet pipe 19; a water outlet pipe 20 is arranged on the box body 1, and an electromagnetic valve 21 is arranged on the water outlet pipe 20; a top cover 22 is hinged on the box body 1; a heating element 23 and a temperature sensor 24 are arranged in the box body 1; the fixing plate is characterized in that through holes are formed in the fixing plate 8, fixing seats are arranged on the supporting plate 6, a plurality of groups of the through holes are arranged, and the number of the fixing seats is equal to that of the through holes and corresponds to that of the through holes one by one.
When the freezing storage tube box is used, a plurality of groups of freezing storage tubes penetrate through a plurality of groups of through holes to be arranged on corresponding fixed seats, then the pull ring 15 pulls the pull rod 14 outwards to press the spring 16, the sealing cover 3 is sleeved on the vertical rod, the lower surface of the sealing cover 3 is tightly attached to the tube opening of the freezing storage tube, then the pull ring 15 is loosened to enable the pull rod 14 to slide under the action of the spring 16 and extend into the clamping groove 18, so that the sealing cover 3 is fixed, after the sealing cover 3 is fixed, the top cover 22 is covered, water bath liquid is added into the box body 1 through 19, the motor 10 is started to enable the motor 10 to drive the eccentric wheel 11 to rotate, the eccentric wheel 11 drives the limiting frame 12 and the sliding rod 13 to slide left and right, the sliding rod 13 drives the fixed plate 8, the connecting rod 7 and the supporting plate 6 to slide left and right to drive the freezing storage tube to slide left and right, so that manual shaking can be replaced, and the freezing storage liquid in the freezing storage tube can be rapidly melted, accelerate the recovery to the cell, at cell recovery's in-process, temperature sensor 24 can monitor the temperature of the water bath liquid in box body 1, when the water bath temperature is lower, can heat the water bath liquid through heating member 23 to make the temperature of water bath liquid can keep 37 ℃, use the back of accepting, close motor 10, start solenoid valve 21, through 20 with water bath liquid discharge can.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
The basic principles and the main features of the invention and the advantages of the invention have been shown and described above, it will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, but that the invention may be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (7)

1. A cell culture freezes water bath box which characterized in that: comprises a box body, a shaking component, a sealing cover and a fixing component, wherein the shaking component is arranged on the box body, the sealing cover is arranged on the shaking component, the fixing component is arranged on the sealing cover, the shaking component comprises a limiting rod, a supporting plate, a connecting rod, a fixed plate, a directional rod, a motor, an eccentric wheel, a limiting frame and a sliding rod, the limiting rod is arranged in the box body, the supporting plate is slidably sleeved on the limiting rod, the connecting rod is arranged on the supporting plate, the fixed plate is arranged on the connecting rod, the sliding rod is arranged on the fixed plate and slidably penetrates through the box body to extend out of the box body, the limiting frame is arranged on the sliding rod, the motor is arranged on the box body, the eccentric wheel is arranged on the motor and rotatably and slidably arranged in the limiting frame, a sleeve is arranged in the box body, the directional rod is arranged on the fixed plate and slidably arranged in the sleeve, a vertical rod is arranged on the fixed plate, the sealing cover is sleeved on the vertical rod in a sliding mode.
2. The cell culture freezing water bath box according to claim 1, wherein: the fixed component comprises a pull rod, a pull ring, a spring and a limiting plate, a sliding cavity is formed in the sealing cover, a clamping groove is formed in the vertical rod, the pull rod slides to penetrate through the sliding cavity and extend into the clamping groove, the limiting plate is arranged on the pull rod and is arranged in the sliding cavity in a sliding mode, the spring is sleeved on the pull rod, the two ends of the spring are arranged on the inner walls of the limiting plate and the sliding cavity respectively, and the pull ring is arranged on the pull rod.
3. The cell culture freezing water bath box according to claim 1, wherein: the box body is provided with a water inlet pipe.
4. The cell culture freezing water bath box according to claim 3, wherein: the box body is provided with a water outlet pipe, and the water outlet pipe is provided with an electromagnetic valve.
5. The cell culture freezing water bath box according to claim 4, wherein: the box body is hinged with a top cover.
6. The cell culture freezing water bath box according to claim 5, wherein: heating members and temperature sensors are arranged in the box body.
7. The cell culture freezing water bath box according to claim 1, wherein: the fixing plate is provided with through holes, the supporting plate is provided with fixing seats, the through holes are provided with a plurality of groups, and the number of the fixing seats is equal to that of the through holes and corresponds to that of the through holes one by one.
CN202121596867.XU 2021-07-14 2021-07-14 Cell culture freezes and deposits water bath box Active CN215103281U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202121596867.XU CN215103281U (en) 2021-07-14 2021-07-14 Cell culture freezes and deposits water bath box

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202121596867.XU CN215103281U (en) 2021-07-14 2021-07-14 Cell culture freezes and deposits water bath box

Publications (1)

Publication Number Publication Date
CN215103281U true CN215103281U (en) 2021-12-10

Family

ID=79320796

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202121596867.XU Active CN215103281U (en) 2021-07-14 2021-07-14 Cell culture freezes and deposits water bath box

Country Status (1)

Country Link
CN (1) CN215103281U (en)

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