CN214622590U - Simple and easy continuous flow's acute toxicity testing arrangement of daphnia class - Google Patents
Simple and easy continuous flow's acute toxicity testing arrangement of daphnia class Download PDFInfo
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- CN214622590U CN214622590U CN202120085500.5U CN202120085500U CN214622590U CN 214622590 U CN214622590 U CN 214622590U CN 202120085500 U CN202120085500 U CN 202120085500U CN 214622590 U CN214622590 U CN 214622590U
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- Prior art keywords
- phase extraction
- extraction column
- hose
- solid
- solid phase
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- 241000238578 Daphnia Species 0.000 title claims abstract description 22
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 title claims abstract description 22
- 238000002414 normal-phase solid-phase extraction Methods 0.000 claims abstract description 60
- 239000007788 liquid Substances 0.000 claims abstract description 23
- 239000004033 plastic Substances 0.000 claims abstract description 22
- 231100000820 toxicity test Toxicity 0.000 claims abstract description 19
- 239000012528 membrane Substances 0.000 claims abstract description 11
- 241001494246 Daphnia magna Species 0.000 claims description 14
- 239000012085 test solution Substances 0.000 claims description 13
- 238000012360 testing method Methods 0.000 claims description 13
- 238000011047 acute toxicity test Methods 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 6
- 238000009825 accumulation Methods 0.000 claims description 4
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- -1 polypropylene Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 231100000041 toxicology testing Toxicity 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 11
- 230000003068 static effect Effects 0.000 abstract description 6
- 239000003440 toxic substance Substances 0.000 abstract description 6
- 231100000614 poison Toxicity 0.000 abstract description 4
- 230000008859 change Effects 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 230000009467 reduction Effects 0.000 abstract description 2
- 206010013496 Disturbance in attention Diseases 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 231100000167 toxic agent Toxicity 0.000 description 3
- 230000001988 toxicity Effects 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 230000009182 swimming Effects 0.000 description 2
- 241000238424 Crustacea Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
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Abstract
A simple and easy continuous flow daphnia class acute toxicity testing device relates to an acute toxicity testing device. Comprises a plastic bottle, a hose, a drip cup, a flow regulator, an equal-diameter straight joint, a solid-phase extraction column adapter, a solid-phase extraction column, a solid-phase extraction membrane and a control valve; the plastic bottle is used for storing toxicity test liquid, the hose is connected with the plastic bottle, the lower end of the hose is connected with the drip cup, the drip cup is used for observing the flow velocity of the liquid, the hose is connected with the drip cup, the flow regulator is embedded in the hose, and the equal-diameter straight joint is connected with the hose and the solid-phase extraction column adapter; the lower end of the solid phase extraction column adapter is connected with a solid phase extraction column; the solid phase extraction column is internally provided with a solid phase extraction membrane, and the lower end of the solid phase extraction column is connected with a control valve. The water body can be updated and flowed, the water body environment is closer to the water body environment under the natural condition, and the problems of reduction of the concentration of toxic substances, change of the components of the exposure liquid and the like in static exposure are solved; the device has simple structure, easily obtained materials and convenient assembly.
Description
Technical Field
The utility model belongs to the environment field relates to an acute toxicity testing arrangement, specifically relates to a simple and easy continuous flow's acute toxicity testing arrangement of daphnia class.
Background
In recent years, the problems of water environment deterioration and water quality pollution are still serious, and the water environment quality becomes the focus of attention. Biological testing is an important means for evaluating aquatic ecological risks of pollutants, is simple in method, does not need special instruments and equipment, and can comprehensively reflect the influence of the pollutants on an ecological system and the pollution condition, so that the biological testing method is widely applied. Acute toxicity testing is a short-term biological test that refers to short-term exposure of a test organism to a toxic substance to understand the magnitude and character of the substance's toxicity. Daphnia organisms such as daphnia magna live in natural waters, belong to animals of daphnia of family Daphnia of family Crustacea, have the characteristics of short growth cycle, quick reproduction, economy, easy cultivation, high sensitivity to poisons and the like, and are internationally recognized standard toxicity test organisms. At present, a laboratory static exposure mode is mainly adopted for testing the toxicity of the daphnia magna, namely, the daphnia magna is placed in a container for exposure, and the exposure liquid cannot be replaced during testing. Problems with this static exposure approach include: the concentration loss of toxic substances in the test process, the influence of biological metabolism on the chemical properties of the water body and the like. These problems can affect the accuracy of the contaminant toxicity assessment. Toxicity testing based on continuous flow exposure is a good solution to the above problems. How to realize the continuous flow of small-size biology such as daphnia class low-cost high-quality and expose toxicity test, it is exactly the utility model discloses the problem that will research and solve.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a simple and easy continuous flow's acute toxicity testing arrangement of daphnia class that can solve static water because of the toxic substance concentration loss that biological absorption, container absorption, solvent volatilize, biological metabolism etc. arouse, expose the unstable scheduling problem of liquid water chemical composition.
The utility model comprises a plastic bottle, a hose, a drip cup, a flow regulator, an equal-diameter direct joint, a solid phase extraction column adapter, a solid phase extraction column, a solid phase extraction membrane and a control valve; the plastic bottle is used for storing toxicity test liquid, the hose is connected with the plastic bottle and used for conveying liquid, the lower end of the hose is connected with a drip cup which is used for observing the flow rate of the liquid, the drip cup is connected with the hose in a lower connection mode, a flow regulator is embedded in the hose, and the equal-diameter straight joint is connected with the hose and a solid-phase extraction column conversion joint; the lower end of the solid phase extraction column adapter is connected with a solid phase extraction column; the solid phase extraction column is internally provided with a solid phase extraction membrane, and the lower end of the solid phase extraction column is connected with a control valve.
The plastic bottle can be provided with a cover with a hanging rope and used for vertically hanging the whole device, and toxicity test liquid is filled in the bottle.
The flow regulator is provided with a pulley, and the flow speed is controlled by adjusting the pulley.
And a certain amount of toxicity test solution is filled in the solid phase extraction column, and the daphnia magna is exposed in the toxicity test solution for acute toxicity test and result observation.
The solid-phase extraction diaphragm is made of polypropylene and is used for preventing daphnia magna from swimming out or flowing out along with the test solution.
The control valve is provided with an adjusting knob for controlling the outflow and accumulation of the test liquid.
The utility model has the advantages that: the daphnia magna acute toxicity testing device can realize the updating and flowing of the water body, is more close to the water body environment under the natural condition, and solves the problems of the reduction of the concentration of toxic substances, the change of the components of the exposure liquid and the like in static exposure; in addition, the device has simple structure, easily obtained materials and convenient assembly.
Drawings
Fig. 1 is a schematic structural diagram of a simple continuous flow daphnia acute toxicity testing device.
Each of the labels in the figure is: 1-plastic bottle; 2, a hose; 3, a drip cup; 4-flow regulator; 5, an equal-diameter straight-through joint; 6-solid phase extraction column adapter; 7-solid phase extraction column; 8-solid phase extraction membrane; 9-control valve.
Detailed Description
The following embodiments will be described in detail with reference to the accompanying drawings.
As shown in fig. 1, the utility model relates to a simple and easy continuous flow's acute toxicity testing arrangement of daphnia class includes: 1-plastic bottle; 2, a hose; 3, a drip cup; 4-flow regulator; 5, an equal-diameter straight-through joint; 6-solid phase extraction column adapter; 7-solid phase extraction column; 8-solid phase extraction membrane; 9-control valve.
The utility model comprises a plastic bottle 1, a hose 2, a drip cup 3, a flow regulator 4, an equal-diameter straight joint 5, a solid phase extraction column adapter 6, a solid phase extraction column 7, a solid phase extraction membrane 8 and a control valve 9; the plastic bottle 1 is used for storing toxicity test liquid, the hose 2 is connected with the plastic bottle 1 and used for conveying liquid, the lower end of the hose 2 is connected with the drip cup 3, the drip cup 3 is used for observing the flow rate of the liquid, the hose is connected with the drip cup 3, the flow regulator 4 is embedded in the hose 2, and the equal-diameter straight joint 5 is connected with the hose 2 and the solid-phase extraction column conversion joint 6; the lower end of the solid phase extraction column adapter 6 is connected with a solid phase extraction column 7; the solid phase extraction column 7 is internally provided with a solid phase extraction membrane 8, and the lower end of the solid phase extraction column 8 is connected with a control valve 9.
The plastic bottle 1 may be provided with a lid 11 with a hanging rope for hanging the whole device vertically, the bottle being filled with a toxicity test solution.
The flow regulator 4 is provided with a pulley, and the flow rate is controlled by adjusting the pulley.
And a certain amount of toxicity test solution is filled in the solid-phase extraction column 7, and the daphnia magna is exposed in the toxicity test solution for acute toxicity test and result observation.
The solid-phase extraction diaphragm 8 is a polypropylene solid-phase extraction diaphragm and is used for preventing daphnia magna from swimming out or flowing out along with the test solution.
The control valve 9 is provided with an adjusting knob for controlling the outflow and accumulation of the test liquid.
The following steps of using the utility model discloses carry out simple and easy continuous flow's daphnia class acute toxicity test:
a. and (3) filling the test solution for the daphnia magna toxicity test into the plastic bottle 1, closing the control valve 9, adjusting the flow regulator 4, extruding the drip cup 3, injecting the test solution into 2/3 in the solid-phase extraction column 7, and adjusting the flow regulator 4 to ensure that the test solution in the plastic bottle 1 does not flow out any more.
b. 10 daphnia magna larvae of about 10 daphnia magna are placed into the solid phase extraction column, the number of the larvae is recorded, the flow regulator 4 is regulated, the control valve 9 is opened, the test solution flows into the solid phase extraction column 7, and the flowing-out speed and the flowing-in speed are equal. Timing is started, and toxicity test experiments are carried out at normal temperature.
c. And observing every 24h within 48h from the beginning of timing, and recording the survival number and survival state of the daphnia magna larvae. From the beginning of the timekeeping, the toxicity test was ended by 48 h.
In addition, if the toxicity test biology is other daphnia class that the size is littleer, the utility model discloses can solve the inconvenient problem of operation of larva size undersize. The concrete solving method is as follows: firstly, adding a culture solution into the plastic bottle 1, adding the culture solution into 2/3 parts of the solid-phase extraction column 7, putting the daphnia parent into the solid-phase extraction column for culturing, taking out the parent after the parent produces the larva, replacing the culture solution with a test solution, and carrying out a toxicity test experiment.
The utility model is used for biological acute toxicity test of small-size tests such as daphnia class, the device includes plastic bottle, hose, drip kettle, flow regulator, constant diameter through joint, solid-phase extraction post crossover sub, solid-phase extraction post, solid-phase extraction diaphragm, control valve. The plastic bottle is used for storing toxicity test liquid, the drip cup and the flow regulator are used for controlling flow speed, a certain amount of toxicity test liquid is stored in the solid-phase extraction column, daphnia is exposed in the solid-phase extraction column, and the control valve controls the test liquid to flow out, so that the continuous flowing and updating of the exposed liquid are realized, the natural water environment is better simulated, and the problems of toxic substance concentration loss, biological metabolite accumulation and the like in a static exposure test are solved. The present invention is not limited to the above embodiments, and any deformation, improvement, and replacement that can be conceived by those skilled in the art can be made without departing from the present invention.
Claims (6)
1. A simple and continuous flowing daphnia acute toxicity testing device is characterized by comprising a plastic bottle, a hose, a drip cup, a flow regulator, an equal-diameter direct joint, a solid-phase extraction column adapter, a solid-phase extraction column, a solid-phase extraction diaphragm and a control valve; the plastic bottle is used for storing toxicity test liquid, the hose is connected with the plastic bottle and used for conveying liquid, the lower end of the hose is connected with a drip cup which is used for observing the flow rate of the liquid, the drip cup is connected with the hose in a lower connection mode, a flow regulator is embedded in the hose, and the equal-diameter straight joint is connected with the hose and a solid-phase extraction column conversion joint; the lower end of the solid phase extraction column adapter is connected with a solid phase extraction column; the solid phase extraction column is internally provided with a solid phase extraction membrane, and the lower end of the solid phase extraction column is connected with a control valve.
2. The simple continuous flow daphnia acute toxicity test device of claim 1, wherein said plastic bottle is provided with a cover with a hanging rope for hanging the whole device vertically.
3. The easy and continuous flow daphnia acute toxicity test device of claim 1, wherein the flow regulator is provided with a pulley for controlling the flow rate by adjusting the pulley.
4. The simple and easy continuous flow daphnia acute toxicity testing device of claim 1, wherein a certain amount of toxicity testing solution is filled in the solid phase extraction column, daphnia magna is exposed in the toxicity testing solution, and acute toxicity testing and result observation are performed.
5. The simple and easy continuous flow daphnia acute toxicity testing device of claim 1, wherein the solid phase extraction membrane is a polypropylene solid phase extraction membrane, and is used for preventing the daphnia magna from moving out or flowing out along with the testing liquid.
6. The simple continuous flow daphnia acute toxicity test device of claim 1, wherein the control valve is provided with an adjusting knob for controlling the outflow and accumulation of the test solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120085500.5U CN214622590U (en) | 2021-01-13 | 2021-01-13 | Simple and easy continuous flow's acute toxicity testing arrangement of daphnia class |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120085500.5U CN214622590U (en) | 2021-01-13 | 2021-01-13 | Simple and easy continuous flow's acute toxicity testing arrangement of daphnia class |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214622590U true CN214622590U (en) | 2021-11-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120085500.5U Expired - Fee Related CN214622590U (en) | 2021-01-13 | 2021-01-13 | Simple and easy continuous flow's acute toxicity testing arrangement of daphnia class |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214622590U (en) |
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2021
- 2021-01-13 CN CN202120085500.5U patent/CN214622590U/en not_active Expired - Fee Related
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Granted publication date: 20211105 |