CN214599035U - Continuous reaction device for cracking, concentrating and separating out solid-phase synthetic peptide resin - Google Patents
Continuous reaction device for cracking, concentrating and separating out solid-phase synthetic peptide resin Download PDFInfo
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- CN214599035U CN214599035U CN202120364760.6U CN202120364760U CN214599035U CN 214599035 U CN214599035 U CN 214599035U CN 202120364760 U CN202120364760 U CN 202120364760U CN 214599035 U CN214599035 U CN 214599035U
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Abstract
The utility model discloses a continuous reaction unit that is used for solid phase synthesis peptide resin schizolysis, concentration and appears. The device comprises a cracking liquid preparation tank, a cracking tank, a concentration and precipitation tank and a solvent tank which are sequentially connected through a corrosion-resistant, acid-resistant and alkali-resistant organic solvent-resistant connecting pipeline provided with a valve bank. The apparatus further includes a cooling circulation system and a vacuum system. The utility model discloses a whole operation process of device once only accomplishes in succession in the system that jar, pyrolysis tank, concentrated analysis jar, solvent jar and relevant valve and pipeline are constituteed at the lysate preparation jar, pyrolysis tank, has both reduced the loss that the in-process material shifts the production, has reduced the danger that the experimenter faces again, and the capacity of every jar body can also be dynamic adjustment according to the size of actual pyrolysis scale in order to adapt to different production scales. Has wide application prospect in the field of solid phase synthesis of peptide.
Description
Technical Field
The utility model relates to a continuous reaction device, in particular to a continuous reaction device for cracking, concentrating and separating out large-scale solid phase synthetic peptide resin.
Background
With the increasing number of polypeptide drugs on the market and the development of solid phase polypeptide synthesis technology, the cleavage reaction of solid phase synthetic peptide resin is more and more common. Although the conventional rotary evaporator can also meet the requirements of the cracking reaction, the repeated liquid transfer during the later filtration and concentration becomes inconvenient and easily causes material loss.
For example, CN110294800A discloses a preparation method of somaglutide, which is prepared by a solid phase coupling reaction of peptide-resin in the presence of a promoter and a condensing agent, and a crude product is obtained by a cracking agent containing trifluoroacetic acid. Trifluoroacetic acid used in the cracking process is a strong acid with a low boiling point, has strong irritation, and can cause physical injury after being contacted.
For another example, CN102532252A discloses a method and an apparatus for high-throughput polypeptide synthesis, the apparatus includes a bottom box with an open top and a reaction plate capable of closing the open top of the bottom box, the side and/or bottom of the bottom box is provided with at least one air inlet and one liquid outlet, the reaction plate is provided with a plurality of reaction chambers arranged in order, each reaction chamber includes an inlet and an outlet located in the inner cavity of the bottom box, and the outlets are closed by sand cores. The process is cumbersome and still requires further optimization.
Therefore, there is still a need for a device, especially a large-scale device, which not only can realize the continuous transfer of liquid in solid phase synthesis and complete the whole process from liquid preparation, lysis to final polypeptide obtaining, but also can avoid the danger of toxic and harmful chemical reagents faced by experimenters. In addition, how to avoid material loss and improve the working efficiency of the continuous process are still problems to be solved.
SUMMERY OF THE UTILITY MODEL
To the deficiency in the prior art, the utility model provides a continuous reaction device that is used for the schizolysis of scale solid phase synthetic peptide resin, concentration and appears. The utility model discloses a device passes through the pipeline and realizes the continuous transfer of liquid under the negative pressure effect, accomplishes from joining in marriage liquid, schizolysis to the overall process that finally obtains the polypeptide. The utility model discloses a continuous reaction unit for cracking, concentrating and separating out large-scale solid phase synthetic peptide resin, which comprises a cracking liquid preparation tank, a cracking tank, a concentration and separation tank and a solvent tank which are sequentially connected through a corrosion-resistant, acid-resistant and alkali-resistant organic solvent-resistant connecting pipeline provided with a valve bank;
the device further comprises a cooling circulation system for pre-cooling and controlling the temperature of the lysate preparation tank, the lysis tank and the concentration and precipitation tank, and a vacuum system for providing power and vacuum for liquid transfer and concentration of the device.
According to the utility model discloses a continuous reaction unit that is used for scale solid phase synthesis peptide resin schizolysis, concentration and appears, preferably, the jar is prepared to the lysate for the cover glass jar that presss from both sides that is provided with agitating unit and scale, and its upper portion is provided with agitating unit, thermometer, inlet and vacuum extraction opening, and the lower extreme is provided with the valve and through the tube coupling pyrolysis jar, utilizes to press from both sides cover recirculated cooling liquid to come the precooling lysate.
According to the utility model discloses a continuous reaction unit that is used for scale solid phase synthesis peptide resin schizolysis, concentration and appears, preferably, the schizolysis jar is for being provided with agitating unit's the cover glass retort that presss from both sides, and its upper portion is agitating unit, thermometer, inlet and vacuum extraction opening, and the lower part is the G2 sand board filter interface who takes the valve, separates out the jar through the pipe connection is concentrated, utilizes to press from both sides cover recirculated cooling liquid to control reaction temperature.
According to the utility model discloses a continuous reaction unit that is used for scale solid phase synthesis peptide resin schizolysis, concentration and appears, preferably, the jar is gone out in concentration for the jacket glass retort that is provided with agitating unit, and its upper portion is condensation recovery unit, agitating unit, thermometer, manometer and inlet, and the lower part is the drain hole that is provided with the valve.
According to the utility model discloses a continuous reaction unit that is used for scale solid phase synthesis peptide resin schizolysis, concentration and appears, preferably, the jacket glass jar of solvent jar for being provided with agitating unit and scale, its upper portion is agitating unit, thermometer, inlet and vacuum extraction opening, and the lower extreme is provided with the valve and goes out the jar through the concentrated analysis of tube coupling, utilizes to press from both sides the jacket recirculated cooling liquid to come the precooling solvent.
According to the utility model discloses a continuous reaction unit that is used for scale solid phase synthesis peptide resin schizolysis, concentration and appears, preferably, the valves is the manual two-way or two three-way valves of PTFE material, is used for cooperating the trend of vacuum system control liquid.
According to the utility model discloses a continuous reaction unit that is used for pyrolysis, concentration of scale solid phase synthesis peptide resin and appears, preferably, cooling circulation system is the cooling water circulation refrigerating unit that can cool down to-20 ℃.
According to the utility model discloses a continuous reaction unit that is used for scale solid phase synthesis peptide resin schizolysis, concentration and appears, preferably, vacuum system is for taking the positive empty pump or the anticorrosive diaphragm vacuum pump of drawing water of preventing suck-back device.
According to the utility model discloses a continuous reaction unit that is used for scale solid phase synthesis peptide resin schizolysis, concentration and appears, preferably, anticorrosive acid and alkali-resistance, resistant organic solvent pipeline are the pipeline of PTFE or PFA material, realize the transfer of liquid through cooperating with the valves.
Utilize the utility model discloses a device, whole operation process once only accomplish in succession in the system that jar, pyrolysis tank, concentration are analysed out jar, solvent jar and relevant valve and pipeline are constituteed at the lysate, both reduced the loss that the in-process material shifts the production, reduced the danger that the experimenter faced again, the capacity of every jar body can also be dynamic adjustment according to the size of actual schizolysis scale in order to adapt to different production scales. Has wide application prospect in the field of solid phase synthesis of peptide.
Drawings
Fig. 1 is the overall pipeline connection diagram of the present invention.
Description of reference numerals:
1 … condenser; 2 … receiver; 3 … thermometer; 4 … pressure gauge; 5 … stirring device;
100 … cracking solution preparation tank; 200 … cracking tank; 300 … concentrating and separating out the tank; 400 … solvent tank; a 500 … valve set; 600 … connecting pipeline; 700 … vacuum system.
Detailed Description
Various exemplary embodiments of the present invention are described in detail below with reference to the accompanying drawings, which should not be considered limiting of the invention, but rather are understood to be more detailed descriptions of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, to the extent that numerical ranges are recited in the present disclosure, it is understood that the upper and lower limits of the range, and each intervening value therebetween, is specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is also encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only the preferred methods and materials are described in this disclosure, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present disclosure. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control. Furthermore, the terms top, bottom, over, under and the like in the description and the claims are used for descriptive purposes and not necessarily for describing relative positions.
[ continuous reaction apparatus ]
The utility model discloses a continuous reaction unit is used for the continuous reaction of scale solid phase synthesis peptide resin schizolysis, concentration and precipitation, and it includes at least that the lysate prepares jar, pyrolysis tank, concentration precipitation jar, solvent jar, valves, cooling circulation system and vacuum system to link into an integrated entity through anticorrosive acid and alkali-resistance, resistant organic solvent pipeline, each part is explained in detail below.
[ preparation tank of lysate ]
The upper part of the cracking liquid preparation tank of the utility model is a stirring device, which is not particularly limited as long as it has a stirring function, for example, the stirring device known in the field can be used. Preferably, the stirring device is connected to the tank cover through a mechanical seal, and the stirring and the uniform mixing of the liquid are realized by using the paddle shaft.
The tank cover is provided with a liquid inlet with a valve for feeding liquid and an air exhaust port connected with a vacuum system for providing negative pressure power. The cover is connected with the tank body, preferably, the jacket tank body is connected with scales through a flange, the proportioning of the lysate is controlled through the scales, and the precooling of the lysate is realized through the connection of the jacket with water bath circulation. The lower end of the tank body is provided with a valve and is connected with the cracking tank through a pipeline, after the prepared cracking liquid is pre-cooled to a set temperature, the valve at the lower end is opened to introduce the cracking liquid into the cracking tank through negative pressure in the cracking tank, the set value of the temperature is not particularly limited and can be adjusted according to the expected experimental purpose of a person skilled in the art.
[ cracking tank ]
The upper part of the cracking tank is provided with a stirring device, preferably, the stirring device is connected to the tank cover through mechanical seal, and the slurry shaft is utilized to realize uniform stirring of the cracking liquid and the resin. The tank cover is provided with a liquid inlet with a valve for feeding liquid and an air exhaust port connected with a vacuum system for providing negative pressure power. The cover is preferably connected with the jacket tank body through a flange, and the temperature control of cracking is realized by connecting the jacket with water bath circulation.
The utility model discloses a jar body lower extreme of pyrolysis tank is for taking the G2 sand board of valve to filter the interface and go out the jar through the concentrated analysis of tube coupling, and G2 sand board filters the interface and indicates the device that contains the aperture and be 10-15 mu m G2 sand board. After cracking is finished, the lower valve is opened to realize the separation of the resin and the lysate through the negative pressure of the concentration and elution tank and to guide the lysate containing the polypeptide into the concentration and elution tank.
[ solvent tank ]
The upper part of the solvent tank is provided with a stirring device, preferably, the stirring device is connected to the tank cover through mechanical seal, and the stirring and the uniform mixing of the liquid are realized by utilizing the paddle shaft. The tank cover is provided with a liquid inlet with a valve for feeding liquid and an air exhaust port connected with a vacuum system for providing negative pressure power. The cover is preferably connected with a jacket tank body with scales through a flange, the addition volume of the precipitated solvent is controlled through the scales, and precooling of the precipitated solvent is realized through the connection of the jacket with water bath circulation. The lower end of the tank body is provided with a valve and is connected with a concentration and precipitation tank through a connecting pipeline, and after the required precipitation solvent is precooled to a set temperature, the valve at the lower end is opened to guide the precipitation solvent into the concentration and precipitation tank through negative pressure in the concentration and precipitation tank.
[ concentration and precipitation tank ]
The upper part of the concentration precipitation tank is provided with a stirring and solvent condensation recovery device, preferably, the stirring device is connected to the tank cover through mechanical seal, and the slurry shaft is utilized to realize uniform stirring of the polypeptide-containing lysate.
The utility model discloses a concentrated tank of appearing includes condensation recovery unit, and condensation recovery unit connects on the tank cap, utilizes vacuum and cooling water circulation system, realizes trifluoroacetic acid's in the lysate recovery. The condensation recovery apparatus is not particularly limited as long as the purpose of condensation recovery can be achieved. Preferably, the condensation and recovery device comprises a condenser and a receiver, which are connected in sequence through a connecting pipeline provided with a valve, and the condenser and the receiver can use the condenser and the receiver which are commercially available or known in the field. Still preferably, the condensation recovery device is connected with the vacuum system through a connecting pipeline.
The inlet that has the valve on the cover of jar is educed in the concentration realizes the feed liquor, and the cover presss from both sides the cover jar body through flange joint, connects the water bath circulation through pressing from both sides the cover and realizes temperature control. The lower part of the tank body is a discharge hole with a valve, and after concentration and precipitation are finished, the material is discharged for post-treatment.
[ vacuum System ]
The vacuum system of the utility model is characterized in that the vacuum system comprises at least one set of air extraction equipment/system which can remove the gas in the tank body to obtain the required vacuum condition. Preferably, the equipment/system at least comprises a vacuum pump, the pumped tank body and the condensation recovery device are connected through a connecting pipeline provided with a valve. Also preferably, the apparatus/system includes, but is not limited to, a screw vacuum pump, roots vacuum pump, rotary vane vacuum pump, and water ring vacuum pump vacuum systems. In certain embodiments, when more than one set of such apparatus/system is selected, two or more vacuum pumps used may constitute a vacuum system in series.
The vacuum system of the present invention further comprises corresponding other elements, such as measuring devices. The measuring device is not particularly limited as long as it can perform the monitoring measurement of the pressure. For example, using a known pressure gauge, manometer, gauge tube, or the like.
[ Cooling circulation System ]
The cooling circulation system of the utility model is used for cooling water circulation refrigerating unit which can be cooled to-20 ℃ and provides precooling and temperature control for at least the cracking liquid preparation tank, the cracking tank and the concentration and separation tank of the utility model;
[ valve set ]
The utility model discloses a valves indicates two valves, three valves, five valves or the valves that are more than five valves of constituteing by the single valve. The number of valves of the valve group is not particularly limited and can be adjusted as required. In certain embodiments, the present invention is a two-valve set comprising a valve connected to a vacuum system and a valve connected to a next-stage tank. In another embodiment, the utility model discloses a valves is three valves, and it includes the valve of being connected by vacuum system, with the valve of next stage jar body coupling and with the valve of last one-level jar body coupling and constitute.
[ connecting piping ]
The utility model discloses a connecting line is used for example being connected between the jar body and the jar body or between jar body and the vacuum system on the one hand. On the other hand is used for carrying liquid, considers the liquid that uses in the solid phase synthesis, the utility model discloses a connecting line preferably uses anticorrosive acid and alkali-resistance, resistant organic solvent connecting line. More preferably, the pipe is made of PTFE or PFA material, and the liquid transfer is realized by matching with the valve block.
Examples
The structure of an exemplary continuous reaction apparatus of the present invention will be described with reference to the accompanying drawings. For convenience of explanation, some components may be omitted in the following drawings. These drawings are for illustrative purposes only and should not be used to narrowly interpret the scope of the claimed invention.
FIG. 1 schematically shows a schematic connection diagram of a continuous reaction apparatus for scale-up of cleavage, concentration and precipitation of a peptide resin for solid phase synthesis. As shown in fig. 1, the system comprises a lysate preparation tank 100, a lysis tank 200, a concentration and precipitation tank 300, a Solvent tank 400, a valve bank 500, a cooling circulation system (not shown in the figure), and a Vacuum system 700, which are connected into a whole through a corrosion-resistant, acid-resistant, alkali-resistant, and organic Solvent-resistant connecting pipeline 600 (wherein Solvent is a Solvent barrel/bottle for providing a Solvent, Vacuum is a Vacuum system, a letter P is a pressure gauge, and a letter T is a thermometer), and the following details of each component and continuous reaction for solid-phase synthesis of peptide resin are described below.
First, the lysate preparation tank 100 of the present invention is used for preparing a lysate of a solid-phase synthetic peptide resin, and the stirring device 5 is provided above the tank, and the stirring device 5 is not particularly limited as long as it has a stirring function, and for example, a stirring device known in the art may be used. The stirring device 5 is connected to the tank cover through mechanical seal, and stirring and mixing of liquid are achieved through the paddle shaft.
The tank cover of the lysate preparation tank 100 is provided with a liquid inlet with a valve for realizing liquid inlet and an air extraction opening connected with a vacuum system 700 for providing negative pressure power. Cover and tank connection through the cover jar body that presss from both sides of flange joint area scale, through scale control lysate ratio, through pressing from both sides the precooling that water bath circulation realized the lysate of cover connection (the utility model discloses a cooling circulation system). The lower end of the tank body is provided with a valve and is connected with the cracking tank 200 through a connecting pipeline 600, and after the prepared cracking liquid is pre-cooled to a set temperature, the lower end valve is opened to guide the cracking liquid into the cracking tank 200 through the negative pressure of the cracking tank 200.
The upper part of the cracking tank 200 is provided with a stirring device 5, the stirring device 5 is connected on a tank cover of the cracking tank 200 through mechanical seal, and the slurry shaft is utilized to realize the uniform stirring of the cracking liquid and the resin. The tank cover is provided with a liquid inlet with a valve for feeding liquid and an air suction port connected with the vacuum system 700 for providing negative pressure power. The cover passes through flange joint and presss from both sides the cover jar body, connects the water bath circulation through pressing from both sides the cover and realizes the temperature control of schizolysis (the utility model discloses a cooling circulation system).
The utility model discloses a jar body lower extreme of 200 jar bodies of pyrolysis tank is for taking the G2 sand board of valve to filter the interface and connect concentrated precipitation jar 300 through connecting tube 600, and G2 sand board filters the interface and is the device that contains the aperture and be 10-15 mu m G2 sand board. After the lysis is completed, the lower valve is opened to separate the resin from the lysate by the negative pressure of the concentration and elution tank 300 and to introduce the lysate containing the polypeptide into the concentration and elution tank 300.
The upper part of the concentration and elution tank 300 is provided with a stirring device 5 and a solvent condensation and recovery device, the stirring device 5 is connected on the cover of the concentration and elution tank 300 through mechanical seal, and the slurry shaft is utilized to realize the uniform stirring of the polypeptide lysate.
The utility model discloses a condensation recovery unit of jar 300 is isolated in concentration connects on the tank cap, utilizes vacuum system 700 and cooling water circulation system, realizes trifluoroacetic acid's in the lysate recovery. The condensation recycling device comprises a condenser 1 and a receiver 2, wherein the condenser 1 and the receiver 2 are connected in sequence through a connecting pipeline 600 provided with a valve, and the condenser 1 and the receiver 2 can be a commercially available condenser and a receiver known in the field. The condensation recycling apparatus is connected to the vacuum system 700 through a connection line 600.
The inlet that has the valve on the cover of concentrated separation jar 300 realizes the feed liquor, and the cover presss from both sides the cover jar body through flange joint, connects the water bath circulation through pressing from both sides the cover and realizes temperature control (the utility model discloses a cooling circulation system). The lower part of the tank body is a discharge hole with a valve, and after concentration and precipitation are finished, the material is discharged for post-treatment.
The upper part of the solvent tank 400 is provided with a stirring device 5, the stirring device 5 is connected on the tank cover of the solvent tank 400 through mechanical seal, and the stirring and the uniform mixing of the liquid are realized by utilizing a propeller shaft. The tank cover is provided with a liquid inlet with a valve for feeding liquid and an air suction port connected with the vacuum system 700 for providing negative pressure power. The cover passes through the cover jar body that presss from both sides of flange joint area scale, through the volume of adding of scale control precipitation solvent, through pressing from both sides the precooling that the cover connection water bath circulation realized precipitation solvent (the utility model discloses a cooling circulation system). The lower end of the tank body is provided with a valve and is connected with the concentration and precipitation tank 300 through a connecting pipeline 600, and after the required precipitation solvent is precooled to the set temperature, the lower end valve is opened to lead the precipitation solvent into the concentration and precipitation tank 300 through the negative pressure in the concentration and precipitation tank.
The vacuum system 700 of the present invention includes a set of evacuation devices/systems that can remove the gas in the evacuated canister to obtain the desired vacuum condition. The apparatus/system includes a vacuum pump (not shown in the figure) connected to the pumped tank bodies (the lysate preparation tank 100, the cracking tank 200, the concentration and elution tank 300, and the solvent tank 400) and the condenser 1 through a connecting pipeline 600 provided with valves. The apparatus/system includes a vacuum system using a screw vacuum pump, roots vacuum pump, rotary vane vacuum pump, or water ring vacuum pump. Alternatively, when more than one set of the above devices/systems is selected, two or more vacuum pumps may be used in series to form a vacuum system.
The vacuum system of the present invention further comprises corresponding other elements, such as measuring devices. The measuring device uses a known pressure gauge 4, manometer, gauge tube, or the like.
The cooling circulation system of the utility model is used for providing precooling and temperature control for the cooling water circulation refrigerating unit (not shown in the figure) which can be cooled to-20 ℃, and for the cracking liquid preparation tank 100, the cracking tank 200, the concentration and precipitation tank 300 and the solvent tank 400 of the utility model, the thermometer 3 which can be arranged for measuring the temperature can be used for real-time monitoring;
in addition. The utility model discloses a valves 500 indicates two valves, three valves, five valves or the valves that are more than five valves of constituteing by the single valve. The number of valves of the valve group can be adjusted according to the requirement. In this embodiment, the utility model discloses can be two valves, it includes by the valve be connected with vacuum system 700 and with the valve of next stage jar body coupling constitute. The utility model discloses the valves is three valves, and it includes the valve of being connected by vacuum system 700, with the valve of lower one-level jar body coupling and with the valve of last one-level jar body coupling and constitute.
The connection pipeline 600 of the present invention is used for connecting between the tank body and the tank body or between the tank body and the vacuum system 700, for example, the lysate preparation tank 100, the lysis tank 200, the concentration precipitation tank 300, and the solvent tank 400. On the other hand is used for carrying liquid, considers the liquid (including acid or alkali and organic solvent) that uses in the solid phase synthesis, the utility model discloses a connecting line 600 uses anticorrosive acid and alkali-resistance, resistant organic solvent pipeline, and it is the pipeline of PTFE or PFA material to realize the transfer of liquid (including acid or alkali and organic solvent) through cooperating with valves 500.
Claims (9)
1. A continuous reaction device for cracking, concentrating and separating out solid phase synthetic peptide resin is characterized by comprising a cracking liquid preparation tank, a cracking tank, a concentration and separation tank and a solvent tank which are sequentially connected through a corrosion-resistant, acid-resistant, alkali-resistant and organic solvent-resistant connecting pipeline with a valve bank;
the device further comprises a cooling circulation system for pre-cooling and controlling the temperature of the cracking liquid preparation tank, the cracking tank and the concentration and precipitation tank, and a vacuum system for providing power and vacuum for liquid transfer and concentration.
2. The continuous reaction apparatus for the cleavage, concentration and precipitation of the solid phase synthetic peptide resin according to claim 1, wherein the lysate preparation tank is a jacketed glass tank provided with a stirring device and scales, the stirring device, a thermometer, a liquid inlet and a vacuum extraction opening are arranged on the upper portion of the lysate preparation tank, a valve is arranged on the lower end of the lysate preparation tank and connected with the lysate tank through a pipeline, and a jacket circulating coolant is used for precooling the lysate.
3. The continuous reaction apparatus for the cleavage, concentration and precipitation of the peptide resin for solid phase synthesis according to claim 2, wherein the cleavage tank is a jacketed glass reaction tank provided with a stirring device, the upper portion of the cleavage tank is provided with a stirring device, a thermometer, a liquid inlet and a vacuum extraction opening, the lower portion of the cleavage tank is provided with a G2 sand plate filter interface with a valve, the cleavage tank is connected with the concentration and precipitation tank through a pipeline, and the reaction temperature is controlled by circulating a cooling liquid through the jacket.
4. The continuous reaction apparatus for cleavage, concentration and precipitation of peptide resin for solid phase synthesis according to claim 3, wherein the concentration and precipitation tank is a jacketed glass reaction tank equipped with a stirring device, the upper part of the reaction tank is equipped with a condensation recovery device, a stirring device, a thermometer, a pressure gauge and a liquid inlet, and the lower part of the reaction tank is equipped with a discharge port equipped with a valve.
5. The continuous reaction apparatus for cleavage, concentration and precipitation of peptide resin for solid phase synthesis according to claim 4, wherein the solvent tank is a jacketed glass tank provided with a stirring device and scales, the upper portion of the tank is provided with a stirring device, a thermometer, a liquid inlet and a vacuum extraction opening, the lower end of the tank is provided with a valve and is connected with the concentration and precipitation tank through a pipeline, and the jacket is used for circulating a cooling liquid to pre-cool the solvent.
6. The continuous reaction apparatus for solid-phase peptide resin cleavage, concentration and elution as claimed in claim 1, wherein the valve set is a manual two-way or two-position three-way valve made of PTFE material for controlling the liquid flow direction by cooperating with the vacuum system.
7. The continuous reaction device for the cracking, concentration and precipitation of the solid-phase synthetic peptide resin as claimed in claim 1, wherein the cooling circulation system is a cooling water circulation refrigerating unit capable of cooling to-20 ℃.
8. The continuous reaction device for the cracking, concentration and precipitation of the solid-phase synthetic peptide resin as claimed in claim 1, wherein the vacuum system is a water-pumping vacuum pump or an anti-corrosion diaphragm vacuum pump with a back suction prevention device.
9. The continuous reaction device for the cracking, concentration and precipitation of the solid-phase synthetic peptide resin as claimed in claim 1, wherein the corrosion-resistant, acid-resistant, alkali-resistant and organic solvent-resistant pipeline is a PTFE or PFA pipeline, and the liquid transfer is realized by matching with the valve bank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120364760.6U CN214599035U (en) | 2021-02-08 | 2021-02-08 | Continuous reaction device for cracking, concentrating and separating out solid-phase synthetic peptide resin |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120364760.6U CN214599035U (en) | 2021-02-08 | 2021-02-08 | Continuous reaction device for cracking, concentrating and separating out solid-phase synthetic peptide resin |
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| CN214599035U true CN214599035U (en) | 2021-11-05 |
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