CN214584883U - A non-invasive detection device for metabolites in embryo culture medium - Google Patents
A non-invasive detection device for metabolites in embryo culture medium Download PDFInfo
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- CN214584883U CN214584883U CN202120361616.7U CN202120361616U CN214584883U CN 214584883 U CN214584883 U CN 214584883U CN 202120361616 U CN202120361616 U CN 202120361616U CN 214584883 U CN214584883 U CN 214584883U
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- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 52
- 238000001514 detection method Methods 0.000 title claims abstract description 27
- 239000002207 metabolite Substances 0.000 title claims abstract description 20
- 239000001963 growth medium Substances 0.000 title abstract 5
- 238000005070 sampling Methods 0.000 claims abstract description 31
- 230000007246 mechanism Effects 0.000 claims abstract description 23
- 238000010926 purge Methods 0.000 claims abstract description 21
- 239000013078 crystal Substances 0.000 claims abstract description 9
- 239000002184 metal Substances 0.000 claims description 11
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 abstract description 6
- 239000007787 solid Substances 0.000 abstract description 5
- 238000005102 attenuated total reflection Methods 0.000 abstract description 4
- 238000001035 drying Methods 0.000 abstract description 3
- 238000012216 screening Methods 0.000 abstract description 3
- 230000003595 spectral effect Effects 0.000 abstract description 3
- 239000000090 biomarker Substances 0.000 abstract description 2
- 238000004566 IR spectroscopy Methods 0.000 abstract 1
- 239000012531 culture fluid Substances 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 13
- 238000002329 infrared spectrum Methods 0.000 description 11
- 238000005516 engineering process Methods 0.000 description 8
- 238000000034 method Methods 0.000 description 5
- 238000007664 blowing Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 210000002257 embryonic structure Anatomy 0.000 description 3
- 238000002513 implantation Methods 0.000 description 3
- 230000002503 metabolic effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 208000000509 infertility Diseases 0.000 description 2
- 231100000535 infertility Toxicity 0.000 description 2
- 238000012827 research and development Methods 0.000 description 2
- SBIBMFFZSBJNJF-UHFFFAOYSA-N selenium;zinc Chemical compound [Se]=[Zn] SBIBMFFZSBJNJF-UHFFFAOYSA-N 0.000 description 2
- 238000010408 sweeping Methods 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 238000001157 Fourier transform infrared spectrum Methods 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000002459 blastocyst Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000012569 chemometric method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002705 metabolomic analysis Methods 0.000 description 1
- 230000001431 metabolomic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
本实用新型提供了一种无创性胚胎培养液代谢物的检测装置，主要由采样附件和弱气流气体吹扫机构构成；所述弱气流气体吹扫机构吹扫的位置为所述采样附件的采样晶体表面。该检测装置置于商业化的傅里叶变换红外光谱仪上，通过将胚胎培养液快速脱水变干得胚胎培养液的固态样品，再以衰减全反射方式利用商业化的傅里叶变换红外光谱仪获得高质量的胚胎培养液红外光谱，从而指导基于光谱生物标志物的胚胎筛选，从而更加精准、便捷地评估胚胎质量。
The utility model provides a non-invasive embryo culture fluid metabolite detection device, which is mainly composed of a sampling accessory and a weak airflow gas purging mechanism; crystal surface. The detection device is placed on a commercial Fourier transform infrared spectrometer, by rapidly dehydrating and drying the embryo culture medium to obtain a solid sample of the embryo culture medium, and then using a commercial Fourier transform infrared spectrometer to obtain a solid sample of the embryo culture medium in the manner of attenuated total reflection. High-quality infrared spectroscopy of embryo culture medium can guide embryo screening based on spectral biomarkers, so as to evaluate embryo quality more accurately and conveniently.
Description
Technical Field
The utility model particularly relates to a detection apparatus for there is not wound embryo culture liquid metabolite.
Background
In recent years, the incidence rate of infertility is remarkably increased, and the birth problems of vast infertility patients are effectively solved by the appearance and development of human assisted reproduction technology. At present, single embryo transplantation is in great tendency, so that how to accurately select high-quality embryos for intrauterine transplantation is a scientific problem which needs to be solved urgently by clinical embryologists. The traditional morphological method adopted at present has large subjective dependence and low evaluation accuracy, and the genetic diagnosis and screening technology before implantation of the subsequently born embryo has high price, complex operation flow and certain limitation for invasive inspection in the field of assisted reproduction.
The embryo culture solution is the environment for the embryo to live and is a unique culture system different from the normal natural pregnant embryo. Metabolomics can fully reflect the dynamic change process of substances through the research on small molecule metabolites. Either combination of the two may help us to assess embryo metabolic activity and developmental potential. However, because the culture solution has small volume (about 10-30 microliter), no good culture solution metabolite detection device is applied to clinic until now.
SUMMERY OF THE UTILITY MODEL
To the problem and the restriction that exist in the background art, the utility model aims to provide a detection device of noninvasive embryo culture liquid metabolite.
The utility model provides a noninvasive embryo culture solution metabolite detection device, which comprises a sampling accessory (1), a weak airflow gas purging mechanism (2) and a metal support column (3) which fixes the weak airflow gas purging mechanism (2) on the sampling accessory (1); the weak gas flow gas purging mechanism (2) purges the surface of the sampling crystal (14) of the sampling accessory (1). The sampling accessory (1) is a sampling accessory of a Fourier transform infrared spectrometer. The detection device can be conveniently placed on a commercial Fourier transform infrared spectrometer to perform noninvasive detection on the metabolites of the embryo culture solution in an attenuated total reflection mode, so as to obtain a high-quality infrared spectrum.
In the device, the weak airflow gas purging mechanism (2) is composed of an air pump (21) and an airflow conduit (22), the air pump (21) pumps airflow to the airflow conduit (22), and the airflow conduit (22) guides airflow to blow to the surface of the sampling crystal (14) of the sampling accessory (1).
In the device, the gas flow conduit (22) of the weak gas flow purging mechanism (2) is arranged on the sampling accessory (1) through a metal support column (3).
In the above device, the number of the air flow ducts (22) is two.
In the device, the power of the air pump (21) is 8W-10W.
In the device, the air flow speed blown out by the weak air flow blowing mechanism (2) is 5L/min-10L/min.
The utility model provides a detection apparatus for there is not wound embryo culture liquid metabolite includes sampling annex, weak air current gas purging mechanism and metal support post. The device is placed on a commercial Fourier transform infrared spectrometer, the noninvasive embryo culture solution metabolite detection device can be used for rapidly dehydrating and drying the embryo culture solution to obtain a solid sample of the embryo culture solution, and then the infrared spectrum of the high-quality embryo culture solution is obtained through the infrared spectrometer, so that the embryo quality is evaluated. It has the following advantages: first, compared with the prior metabolite detection technology, the infrared spectrometer has the advantages of low price, high performance and contribution to large-scale popularization. Secondly, the detection method is limited to be promoted because the volume of the embryo culture liquid is small in the past, the infrared spectrum for effectively describing the characteristics of the embryo culture liquid can be obtained only by 0.5-1 microliter of the culture liquid, the demand for the culture liquid is reduced, and the subsequent operation and research are facilitated. Thirdly, a weak airflow micro air pump is additionally arranged, and the embryo culture solution can be quickly dehydrated and dried on the premise of not blowing away the micro culture solution, so that the infrared spectrum of the embryo culture solution dry sample with high quality (high signal-to-noise ratio and high repeatability) is obtained. Due to the high signal-to-noise ratio and the high repeatability of the infrared spectrum, the method is favorable for reliably detecting the small metabolic difference of the components of the embryo culture solution under different conditions. The research and development of the technology are helpful to promote the development and application of a noninvasive embryo pre-implantation metabolite detection technology, and the high-quality embryos can be screened more accurately, simply and conveniently.
Drawings
Fig. 1 is a schematic view of the structure of the device of the present invention.
Fig. 2 is a schematic left view of the device of the present invention.
Wherein, 1 is a sampling accessory which realizes the measurement of Fourier transform infrared spectrum in an attenuated total reflection mode. In the internal structure of the accessory, 11 is a reflector, 12 is an infrared detection light beam, 13 is a ZnSe lens, 14 is a sampling crystal, 15 is an embryo culture solution, 2 is a weak airflow gas purging mechanism, 21 is an air pump, 22 is an airflow conduit and 3 is a metal support column.
Detailed Description
The present invention will be described in further detail with reference to specific embodiments, which are given for the purpose of illustration only and are not intended to limit the scope of the invention. The following examples are provided as guidelines for further improvement by one of ordinary skill in the art and are not to be construed as limiting the invention in any way.
The experimental procedures in the following examples are conventional unless otherwise specified. Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.
Example 1
The non-invasive detection device for the metabolites of the embryo culture solution provided by the embodiment has the main view structure as shown in fig. 1, and the left view structure as shown in fig. 2: the device comprises a sampling accessory (1), a weak airflow gas purging mechanism (2) and a metal supporting column (3) for fixing the weak airflow gas purging mechanism (2) on the sampling accessory (1); the position swept by the weak gas flow sweeping mechanism (2) is the surface of a sampling crystal (14) of the sampling accessory (1).
In this embodiment, the gas flow conduit (22) of the weak gas flow purging mechanism (2) is mounted on the sampling attachment (1) through a metal support pillar (3).
In this embodiment, the gas flow conduit (22) of the weak gas flow purging mechanism (2) is mounted on the sampling attachment (1) through a metal support pillar (3).
In this embodiment, the number of the air flow ducts (22) is two.
In this embodiment, the power of the air pump is 9W.
In the embodiment, the air flow speed blown out by the weak air flow gas purging mechanism (2) is 8L/min.
In this embodiment, the included angle between the airflow duct (22) and the metal supporting column (3) is 0 degree.
The noninvasive embryo culture solution metabolite detection device is applied, embryo culture solution (15) on the surface of a sampling crystal (14) of a sampling accessory (1) is dried, an infrared detection light beam (12) is reflected by a reflector (11), and infrared spectrum detection is performed by a ZnSe lens (13). The specific experimental method is as follows: dripping a trace amount of embryo culture solution 15 (about 1 microliter) on a sampling crystal (14) of a sampling accessory (1), and rapidly blowing for 5min by using a weak airflow gas purging mechanism (2) to obtain a layer of embryo culture solution dry film; and then taking the layer of embryo culture solution dry film as a solid sample for infrared spectrum collection. And (3) performing spectral analysis on the collected spectrum of a large amount of embryo culture solution, and guiding the screening of the spectral biomarkers according to the blastocyst formation rate, pregnancy outcome and the like by combining a chemometric method. The result shows that the sample to be detected of the culture solution obtained by the method meets the detection requirement, the high-quality spectral quality is finally obtained, and some differences of the culture solutions from two different sources are displayed in a specific area.
The utility model provides a detection apparatus for there is not wound embryo culture liquid metabolite does not have the metal support column that the mechanism was swept to the gaseous mechanism and fixed weak air current of sweeping of sampling annex, weak air current. The noninvasive detection device for the metabolites of the embryo culture solution can be used for obtaining a solid sample of the embryo culture solution by quickly dehydrating and drying the embryo culture solution, and then obtaining a high-quality embryo culture solution infrared spectrum by a Fourier transform infrared spectrometer in an attenuated total reflection mode, thereby evaluating the embryo quality. It has the following advantages: first, compared with the prior metabolite detection technology, the infrared spectrometer has the advantages of low price, high performance and contribution to large-scale popularization. Secondly, the detection method is limited to be promoted because the volume of the embryo culture liquid is small in the past, the infrared spectrum for effectively describing the characteristics of the embryo culture liquid can be obtained only by 0.5-1 microliter of the culture liquid, the demand for the culture liquid is reduced, and the subsequent operation and research are facilitated. Thirdly, a weak airflow micro air pump is additionally arranged, and the embryo culture solution can be quickly dehydrated and dried on the premise of not blowing away the micro culture solution, so that the infrared spectrum of the embryo culture solution dry sample with high quality (high signal-to-noise ratio and high repeatability) is obtained. Due to the high signal-to-noise ratio and the high repeatability of the infrared spectrum, the method is favorable for reliably detecting the small metabolic difference of the components of the embryo culture solution under different conditions. The research and development of the technology are helpful to promote the development and application of a noninvasive embryo pre-implantation metabolite detection technology, and the high-quality embryos can be screened more accurately, simply and conveniently.
The above details the present invention. It will be apparent to those skilled in the art that the present invention can be practiced in a wide range of equivalent parameters and conditions without departing from the spirit and scope of the invention and without undue experimentation. While the present invention has been described with reference to particular embodiments, it will be understood that further modifications may be made. In summary, this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art. The use of some of the essential features is possible within the scope of the claims attached below.
Claims (4)
1. A detection device for non-invasive embryo culture solution metabolites is characterized in that: the device comprises a sampling accessory (1), a weak airflow gas purging mechanism (2) and a metal supporting column (3) for fixing the weak airflow gas purging mechanism (2) on the sampling accessory (1); the weak gas flow gas purging mechanism (2) purges the surface of the sampling crystal (14) of the sampling accessory (1).
2. The apparatus of claim 1, wherein: the weak airflow gas purging mechanism (2) is composed of an air pump (21) and an airflow guide pipe (22), wherein the air pump (21) pumps airflow to the airflow guide pipe (22), and the airflow guide pipe (22) guides airflow to blow to the surface of the sampling crystal (14) of the sampling accessory (1).
3. The apparatus of claim 2, wherein: and the gas flow conduit (22) of the weak gas flow purging mechanism (2) is arranged on the sampling accessory (1) through a metal support column (3).
4. The apparatus of claim 2 or 3, wherein: the number of the airflow conduits (22) is two.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202120361616.7U CN214584883U (en) | 2021-02-09 | 2021-02-09 | A non-invasive detection device for metabolites in embryo culture medium |
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| CN202120361616.7U CN214584883U (en) | 2021-02-09 | 2021-02-09 | A non-invasive detection device for metabolites in embryo culture medium |
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| CN202120361616.7U Active CN214584883U (en) | 2021-02-09 | 2021-02-09 | A non-invasive detection device for metabolites in embryo culture medium |
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2021
- 2021-02-09 CN CN202120361616.7U patent/CN214584883U/en active Active
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