CN206431046U - A kind of measurement apparatus of rice leaf tissue optical property parameter - Google Patents

A kind of measurement apparatus of rice leaf tissue optical property parameter Download PDF

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Publication number
CN206431046U
CN206431046U CN201720021180.0U CN201720021180U CN206431046U CN 206431046 U CN206431046 U CN 206431046U CN 201720021180 U CN201720021180 U CN 201720021180U CN 206431046 U CN206431046 U CN 206431046U
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China
Prior art keywords
rice leaf
leaf tissue
measurement apparatus
property parameter
optical property
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CN201720021180.0U
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Chinese (zh)
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岑海燕
李晓冉
翁海勇
何勇
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Zhejiang University ZJU
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Zhejiang University ZJU
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Abstract

The utility model discloses a kind of measurement apparatus of rice leaf tissue optical property parameter, including light-source system, simple integral ball light path system and the detecting system connected by optical fiber, detecting system connection computer, described light-source system includes:Wave band is 250~2500nm halogen tungsten lamp source;Described simple integral ball light path system includes:Integrating sphere;Hot spot adjuster, on integrating sphere, including metal tube and/or lens, the spot size for controlling optical fiber.Measurement apparatus light source of the present utility model uses halogen tungsten lamp source and hot spot adjuster with continuous wave band, solves the problem of existing measurement apparatus needs switching different-waveband light source in measurement.

Description

A kind of measurement apparatus of rice leaf tissue optical property parameter
Technical field
The utility model is related to organism optical research field, more particularly to a kind of rice leaf tissue optical property parameter Measurement apparatus.
Background technology
The light physiology of communication process and path and organizer, pathological state in biological tissue's body is closely related, as people Study the emphasis of concern.
Optical properties of tissue, is a series of physical quantitys for being used for describing light propagation state in organizer.Light is mixed Propagated in turbid tissue and relate generally to three basic physical processes:Reflect (refraction), absorb and scatter.The decay master of light in the tissue If comprehensive function of the organizer to two kinds of processes of absorption and scattering of light, uses absorption coefficient μ respectivelyaWith scattering coefficient μsRepresent. μaAnd μsAbsorption and scattering power of the organizer to light, wherein μ are described respectivelyaDepend primarily on the chemical composition of tissue;And μsBy To the influence of institutional framework/physical characteristic (such as density, tissue particles size, eucaryotic cell structure).
By the measure to each optical property parameter in organizer, it just can indirectly obtain giving birth to accordingly in organizer Reason, pathological information.Therefore the optical property parameter of organizer is in fields such as crops nutrition diagnosis, Defect inspection, attributional analysises There is very important application value.
At present, optical property parameter has a wide range of applications in terms of the Non-Destructive Testing of fruit.Plant leaf blade tissue is different In fruit tissue, few documents are related to the optical property parameter and its nutrient information or disease with blade of plant leaf blade Relation between evil stress.Paddy rice is the main cereal crops of China, by the optical property parameter of rice leaf to its nutrient Diagnosis, Defect inspection, attributional analysis etc. have great importance.
Publication No. CN103940766A Chinese invention patent document discloses a kind of agricultural product group based on integrating sphere Knit optical characteristics automatic detection device, a kind of farm product tissue optical characteristics detection means, using stepper motor and control system Multiple single-range LASER Light Sources are switched over, obtained after the reflectivity of Kiwi berry, transmissivity using IAD algorithms (reversely times Addition Inverse Adding-doubling, IAD) inverting is carried out to it obtain optical property parameter.Said apparatus is using sharp Radiant can not obtain the optical property parameter under continuous wave band, and IAD inversion algorithms assume that biological tissue is uniform substance, survey Uniform illumination during amount, but above-mentioned condition can not be met in actual applications, therefore can not realize to rice leaf optical characteristics ginseng Several accurate invertings.
Utility model content
The utility model provides a kind of measurement apparatus of rice leaf tissue optical property parameter, and light source, which uses to have, to be connected The halogen tungsten lamp source and hot spot adjuster of continuous wave band, solve existing measurement apparatus needs to switch different-waveband light source in measurement The problem of.
Including the light-source system, simple integral ball light path system and detecting system connected by optical fiber, detecting system connection meter Calculation machine, described light-source system includes:Wave band is 250~2500nm halogen tungsten lamp source;
Described simple integral ball light path system includes:
Integrating sphere;
Hot spot adjuster, on integrating sphere, including metal tube and/or lens, the spot size for controlling optical fiber.
The light source of existing tissue optical property detection means is multiple single-range LASER Light Sources, the needs pair in measurement Multiple single-range LASER Light Sources are switched over, cumbersome, and the light source of measurement apparatus of the present utility model, which is used, to be had continuously The halogen tungsten lamp source of wave band, solves the problem of existing measurement apparatus needs switching different-waveband light source in measurement;This practicality New measurement apparatus employs hot spot adjuster, makes to be radiated on rice leaf tissue sample during measurement reflectivity and transmissivity Spot size be consistent, it is ensured that the diffusing reflection rate of rice leaf tissue and accurately measuring for diffusing transmission rate.
When measuring the transmissivity of rice leaf tissue, described hot spot adjuster is lens, is arranged close to optical fiber The specimen port of integrating sphere side.
When measuring the reflectivity of rice leaf tissue, described hot spot adjuster includes metal tube and lens, described Metal tube is extend into inside integrating sphere by the light inlet of the integrating sphere side away from specimen port, and described lens are arranged on metal One end being located inside integrating sphere of pipe.
When measuring transmissivity and the reflectivity of rice leaf tissue, by the regulation of hot spot adjuster, Rice Leaf is radiated at It is 1mm that spot size on piece tissue samples, which is consistent,.
Preferably, the internal diameter of described metal tube is 8mm, and external diameter 12mm, the length of metal tube is 300mm.
The internal diameter of metal tube is 8mm, external diameter 12mm, when length is 300mm, spot diameter can be made to be maintained at 1mm or so, Make rice leaf tissue diffusing reflection rate and diffusing transmission rate measurement accuracy it is higher.
Preferably, the outer surface of described metal tube scribbles highly-reflective coating.
Preferably, the translucent cover that described detecting system includes detection spectrometer and covered on outside detection spectrometer.
In measurement, translucent cover can avoid being fluctuated the signal detected because of air flow, make testing result It is more accurate.
Compared with prior art, the beneficial effects of the utility model are:
(1) the utility model has built the in vitro optical property parameter measurement apparatus of brand-new simple integral ball, selected light Source, optical fiber, spectrometer, which can guarantee that, can obtain rice leaf in accurately diffusing reflection rate and the diffusing transmission of 350~1000nm wave bands Rate;The lens and hot spot adjuster of setting ensure that the accuracy of measurement;
(2) light source of measurement apparatus of the present utility model uses the halogen tungsten lamp source with continuous wave band, solves existing The problem of measurement apparatus needs switching different-waveband light source in measurement;Measurement apparatus of the present utility model employs hot spot regulation Device, is consistent the spot size being radiated at during measurement reflectivity and transmissivity on rice leaf tissue sample, it is ensured that paddy rice The diffusing reflection rate of leaf tissue and accurately measuring for diffusing transmission rate.
Brief description of the drawings
Fig. 1 is the structural representation of the measurement apparatus of the utility model rice leaf tissue optical property parameter;
Structural representation when Fig. 2 is the transmissivity of measurement rice leaf tissue inside integrating sphere;
Structural representation when Fig. 3 is the reflectivity of measurement rice leaf tissue inside integrating sphere;
Fig. 4 is the schematic flow sheet of the inversion method of the utility model rice leaf tissue optical property parameter.
Wherein, 1, light-source system;2nd, optical fiber;3rd, simple integral ball light path system;4th, detecting system;5th, computer;6th, thoroughly Mirror;7th, adapter;8th, support frame;9th, metal tube;10th, rice leaf tissue sample.
Embodiment
As shown in figure 1, the measurement apparatus of rice leaf tissue optical property parameter of the present utility model mainly includes:
The list that light-source system 1, the diffusing reflection rate to sample and the diffusing transmission rate for providing light source for experimental system are detected Integrating sphere light path system 3, the optical signal in experiment switched to electric signal detecting system 4, data display and processing computer 5;Light-source system 1, simple integral ball light path system 3 and detecting system 4 are 380~1000nm by a diameter of 400 μm, wavelength band Optical fiber 2 connect, detecting system 4 connection computer 5.
The power supply service tank group that the halogen tungsten lamp source and rated power that light-source system 1 is 250~2500nm by wave band are 250W Into its light source power is adjustable, and intensity mode, current-mode or power mode, and the ginseng tested every time may be selected according to demand Number setting can be stored, and experiment next time can be directly invoked during parameter setting;
Simple integral ball light path system 3 includes:
Integrating sphere, its inwall scribbles highly-reflective coating, and such as Spectraflect white coatings can be formed uniformly in inwall Light intensity;
Hot spot adjuster, is 8mm by single lens 6 and/or internal diameter, and external diameter is 12mm, and length is 300mm metal tube 9 Composition, the outer surface of metal tube 9 scribble with integrating sphere inwall identical highly-reflective coating, lens are convex lens.
As shown in Fig. 2 when measuring the transmissivity of rice leaf tissue, hot spot adjuster is lens 6, passes through adapter 7 The specimen port of the integrating sphere side of optical fiber 2 is arranged close to, adapter 7 is arranged on integrating sphere by support frame 8.
As shown in figure 3, when measuring the reflectivity of rice leaf tissue, hot spot adjuster includes metal tube 9 and lens 6, Metal tube 9 is extend into inside integrating sphere by the light inlet of the integrating sphere side away from specimen port, and metal tube 9 is consolidated by adapter 7 Fixed, adapter 7 is arranged on integrating sphere by support frame 8, and lens 6 are arranged on one end being located inside integrating sphere of metal tube 9.
Lens 6 and metal tube 9 are used for the hot spot for adjusting optical fiber 2, and it is 1mm to make to be radiated at the spot size on sample.
Specimen holder, including one end are fixed, and the moveable metal frame of the other end, the two ends of metal frame are fixed with quartz slide, The moveable one end of metal frame can be adjusted according to the thickness of sample;
Support frame 8, for fixing optical fiber, metal tube and specimen holder;
Detecting system 4 includes:Wavelength band is 350~1000nm marine optics detector, and is located at ocean light Learn the translucent cover outside detector;
Computer 5, is analyzed and processed for data acquisition, and to the data collected, is realized to rice leaf tissue Optical property parameter is reconstructed.
As shown in figure 4, as follows to the inversion method of rice leaf tissue optical property parameter based on above-mentioned measurement apparatus:
(1) optical property parameter simulated data sets (μ is seta, μs), wherein μaFor absorption coefficient, μaFrom 0.01cm-1Start With 1cm-1Step-length increase to 20cm-1, totally 20 data;μsFor scattering coefficient, μsFrom 1cm-1Start with 33cm-1Step-length increase Add to 1000cm-1, totally 30 data form 20 × 30 totally 600 groups of data, it is 1 to set anisotropy factor g;Utilize Meng Teka Sieve simulation program, obtains diffusing reflection rate, the diffusing transmission rate (R of the corresponding sample of the simulated data setsd, Td), wherein, RdIt is anti-to overflow Penetrate rate, TdFor diffusing transmission rate.600 groups of (μa, μs) and corresponding (Rd, Td) constitute tranining database;
(2) configuration has fixed optical property parameter (μa', μs') and g one group of standard imitate body, standard of the present utility model Imitative body is the solution of fat emulsion and dyestuff.Its corresponding diffusing reflection rate is obtained using measurement apparatus of the present utility model and unrestrained saturating Penetrate rate (Rd', Td'), it is compared with the analog quantity in tranining database, tranining database is corrected;
(3) build initialization BP neural network, including input layer, the first hidden layer, the second hidden layer and output layer, input layer and Output layer has 600 nodes, and the first hidden layer and the second hidden layer have 25 nodes, the transmission of the first hidden layer and the second hidden layer " tansing " function and " purelin " function is respectively adopted in function, and learning function uses " learngdm " function, network training Middle to use " traingd " function, performance function " mse " evaluates the performance of BP neural network with mean square error.
The input signal of input layer is (Rd, Td), i.e. the diffusing reflection rate of sample and diffusing transmission rate, the output signal of output layer For (μa, μs), i.e. the optical property parameter absorption coefficient and scattering coefficient of sample;
The BP neural network of foundation is trained using the tranining database after correction, until error of fitting is less than set The threshold value put, the threshold value can be set according to actual needs;BP neural network after being trained;
What BP neural network was trained concretely comprises the following steps:
1) tranining database that DSMC is set up is used for the training signal of neutral net error signal.Determine defeated Enter the input of each unit in signal, connection weight and threshold calculations hidden layer, then calculated with the input of each unit by transmission function The output of each unit in hidden layer;
2) using the input of the output of hidden layer, connection weight and threshold calculations output layer each unit, transmission function is then utilized Calculate the corresponding output of output layer each unit;
3) using theoretical output, the reality output of network calculates the vague generalization error of each unit of output layer;
4) using connection weight, the vague generalization error of output layer and the output of hidden layer, the vague generalization error of hidden layer is calculated;
5) connection weight and threshold value are corrected using the output of the vague generalization error and hidden layer of output layer each unit.
(4) measurement parameter (R of rice leaf tissue to be measured is measured using measurement apparatus of the present utility modeld, Td), as BP neural network after input quantity input training, BP neural network output after training relative to rice leaf tissue to be measured Optical property parameter (μa, μs), it is achieved thereby that the anti-structure of the optical property parameter to rice leaf tissue.
The technical solution of the utility model and beneficial effect are described in detail embodiment described above, it should be understood that Be that the foregoing is only specific embodiment of the utility model, be not limited to the utility model, it is all in the utility model Spirit in any modification, supplement and the equivalent substitution made etc., should be included in protection domain of the present utility model it It is interior.

Claims (6)

1. a kind of measurement apparatus of rice leaf tissue optical property parameter, including the light-source system, the list that are connected by optical fiber are long-pending Bulb separation light path system and detecting system, detecting system connection computer, it is characterised in that
Described light-source system includes:Wave band is 250~2500nm halogen tungsten lamp source;
Described simple integral ball light path system includes:
Integrating sphere;
Hot spot adjuster, on integrating sphere, including metal tube and/or lens, the spot size for controlling optical fiber.
2. the measurement apparatus of rice leaf tissue optical property parameter according to claim 1, it is characterised in that work as measurement During the transmissivity of rice leaf tissue, described hot spot adjuster is lens, is arranged close to the sample of the integrating sphere side of optical fiber This mouth.
3. the measurement apparatus of rice leaf tissue optical property parameter according to claim 1, it is characterised in that work as measurement During the reflectivity of rice leaf tissue, described hot spot adjuster includes metal tube and lens, and described metal tube passes through remote The light inlet of the integrating sphere side of specimen port is extend into inside integrating sphere, and described lens are located at integrating sphere installed in metal tube Internal one end.
4. the measurement apparatus of the rice leaf tissue optical property parameter according to claim 1 or 3, it is characterised in that institute The internal diameter for the metal tube stated is 8mm, external diameter 12mm, and the length of metal tube is 300mm.
5. the measurement apparatus of rice leaf tissue optical property parameter according to claim 4, it is characterised in that described The outer surface of metal tube scribbles highly-reflective coating.
6. the measurement apparatus of rice leaf tissue optical property parameter according to claim 1, it is characterised in that described The translucent cover that detecting system includes detection spectrometer and covered on outside detection spectrometer.
CN201720021180.0U 2017-01-09 2017-01-09 A kind of measurement apparatus of rice leaf tissue optical property parameter Expired - Fee Related CN206431046U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106770047A (en) * 2017-01-09 2017-05-31 浙江大学 The inversion method and measurement apparatus of a kind of rice leaf tissue optical property parameter
CN110596098A (en) * 2019-08-26 2019-12-20 江苏大学 Rapid detection system and detection method for ratio distribution of chlorophyll and lutein in cucumber leaves

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106770047A (en) * 2017-01-09 2017-05-31 浙江大学 The inversion method and measurement apparatus of a kind of rice leaf tissue optical property parameter
CN110596098A (en) * 2019-08-26 2019-12-20 江苏大学 Rapid detection system and detection method for ratio distribution of chlorophyll and lutein in cucumber leaves
CN110596098B (en) * 2019-08-26 2022-05-20 江苏大学 Rapid detection system and detection method for ratio distribution of chlorophyll and lutein in cucumber leaves

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