CN206431046U - A kind of measurement apparatus of rice leaf tissue optical property parameter - Google Patents
A kind of measurement apparatus of rice leaf tissue optical property parameter Download PDFInfo
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- CN206431046U CN206431046U CN201720021180.0U CN201720021180U CN206431046U CN 206431046 U CN206431046 U CN 206431046U CN 201720021180 U CN201720021180 U CN 201720021180U CN 206431046 U CN206431046 U CN 206431046U
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- rice leaf
- leaf tissue
- measurement apparatus
- property parameter
- optical property
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Abstract
The utility model discloses a kind of measurement apparatus of rice leaf tissue optical property parameter, including light-source system, simple integral ball light path system and the detecting system connected by optical fiber, detecting system connection computer, described light-source system includes:Wave band is 250~2500nm halogen tungsten lamp source;Described simple integral ball light path system includes:Integrating sphere;Hot spot adjuster, on integrating sphere, including metal tube and/or lens, the spot size for controlling optical fiber.Measurement apparatus light source of the present utility model uses halogen tungsten lamp source and hot spot adjuster with continuous wave band, solves the problem of existing measurement apparatus needs switching different-waveband light source in measurement.
Description
Technical field
The utility model is related to organism optical research field, more particularly to a kind of rice leaf tissue optical property parameter
Measurement apparatus.
Background technology
The light physiology of communication process and path and organizer, pathological state in biological tissue's body is closely related, as people
Study the emphasis of concern.
Optical properties of tissue, is a series of physical quantitys for being used for describing light propagation state in organizer.Light is mixed
Propagated in turbid tissue and relate generally to three basic physical processes:Reflect (refraction), absorb and scatter.The decay master of light in the tissue
If comprehensive function of the organizer to two kinds of processes of absorption and scattering of light, uses absorption coefficient μ respectivelyaWith scattering coefficient μsRepresent.
μaAnd μsAbsorption and scattering power of the organizer to light, wherein μ are described respectivelyaDepend primarily on the chemical composition of tissue;And μsBy
To the influence of institutional framework/physical characteristic (such as density, tissue particles size, eucaryotic cell structure).
By the measure to each optical property parameter in organizer, it just can indirectly obtain giving birth to accordingly in organizer
Reason, pathological information.Therefore the optical property parameter of organizer is in fields such as crops nutrition diagnosis, Defect inspection, attributional analysises
There is very important application value.
At present, optical property parameter has a wide range of applications in terms of the Non-Destructive Testing of fruit.Plant leaf blade tissue is different
In fruit tissue, few documents are related to the optical property parameter and its nutrient information or disease with blade of plant leaf blade
Relation between evil stress.Paddy rice is the main cereal crops of China, by the optical property parameter of rice leaf to its nutrient
Diagnosis, Defect inspection, attributional analysis etc. have great importance.
Publication No. CN103940766A Chinese invention patent document discloses a kind of agricultural product group based on integrating sphere
Knit optical characteristics automatic detection device, a kind of farm product tissue optical characteristics detection means, using stepper motor and control system
Multiple single-range LASER Light Sources are switched over, obtained after the reflectivity of Kiwi berry, transmissivity using IAD algorithms (reversely times
Addition Inverse Adding-doubling, IAD) inverting is carried out to it obtain optical property parameter.Said apparatus is using sharp
Radiant can not obtain the optical property parameter under continuous wave band, and IAD inversion algorithms assume that biological tissue is uniform substance, survey
Uniform illumination during amount, but above-mentioned condition can not be met in actual applications, therefore can not realize to rice leaf optical characteristics ginseng
Several accurate invertings.
Utility model content
The utility model provides a kind of measurement apparatus of rice leaf tissue optical property parameter, and light source, which uses to have, to be connected
The halogen tungsten lamp source and hot spot adjuster of continuous wave band, solve existing measurement apparatus needs to switch different-waveband light source in measurement
The problem of.
Including the light-source system, simple integral ball light path system and detecting system connected by optical fiber, detecting system connection meter
Calculation machine, described light-source system includes:Wave band is 250~2500nm halogen tungsten lamp source;
Described simple integral ball light path system includes:
Integrating sphere;
Hot spot adjuster, on integrating sphere, including metal tube and/or lens, the spot size for controlling optical fiber.
The light source of existing tissue optical property detection means is multiple single-range LASER Light Sources, the needs pair in measurement
Multiple single-range LASER Light Sources are switched over, cumbersome, and the light source of measurement apparatus of the present utility model, which is used, to be had continuously
The halogen tungsten lamp source of wave band, solves the problem of existing measurement apparatus needs switching different-waveband light source in measurement;This practicality
New measurement apparatus employs hot spot adjuster, makes to be radiated on rice leaf tissue sample during measurement reflectivity and transmissivity
Spot size be consistent, it is ensured that the diffusing reflection rate of rice leaf tissue and accurately measuring for diffusing transmission rate.
When measuring the transmissivity of rice leaf tissue, described hot spot adjuster is lens, is arranged close to optical fiber
The specimen port of integrating sphere side.
When measuring the reflectivity of rice leaf tissue, described hot spot adjuster includes metal tube and lens, described
Metal tube is extend into inside integrating sphere by the light inlet of the integrating sphere side away from specimen port, and described lens are arranged on metal
One end being located inside integrating sphere of pipe.
When measuring transmissivity and the reflectivity of rice leaf tissue, by the regulation of hot spot adjuster, Rice Leaf is radiated at
It is 1mm that spot size on piece tissue samples, which is consistent,.
Preferably, the internal diameter of described metal tube is 8mm, and external diameter 12mm, the length of metal tube is 300mm.
The internal diameter of metal tube is 8mm, external diameter 12mm, when length is 300mm, spot diameter can be made to be maintained at 1mm or so,
Make rice leaf tissue diffusing reflection rate and diffusing transmission rate measurement accuracy it is higher.
Preferably, the outer surface of described metal tube scribbles highly-reflective coating.
Preferably, the translucent cover that described detecting system includes detection spectrometer and covered on outside detection spectrometer.
In measurement, translucent cover can avoid being fluctuated the signal detected because of air flow, make testing result
It is more accurate.
Compared with prior art, the beneficial effects of the utility model are:
(1) the utility model has built the in vitro optical property parameter measurement apparatus of brand-new simple integral ball, selected light
Source, optical fiber, spectrometer, which can guarantee that, can obtain rice leaf in accurately diffusing reflection rate and the diffusing transmission of 350~1000nm wave bands
Rate;The lens and hot spot adjuster of setting ensure that the accuracy of measurement;
(2) light source of measurement apparatus of the present utility model uses the halogen tungsten lamp source with continuous wave band, solves existing
The problem of measurement apparatus needs switching different-waveband light source in measurement;Measurement apparatus of the present utility model employs hot spot regulation
Device, is consistent the spot size being radiated at during measurement reflectivity and transmissivity on rice leaf tissue sample, it is ensured that paddy rice
The diffusing reflection rate of leaf tissue and accurately measuring for diffusing transmission rate.
Brief description of the drawings
Fig. 1 is the structural representation of the measurement apparatus of the utility model rice leaf tissue optical property parameter;
Structural representation when Fig. 2 is the transmissivity of measurement rice leaf tissue inside integrating sphere;
Structural representation when Fig. 3 is the reflectivity of measurement rice leaf tissue inside integrating sphere;
Fig. 4 is the schematic flow sheet of the inversion method of the utility model rice leaf tissue optical property parameter.
Wherein, 1, light-source system;2nd, optical fiber;3rd, simple integral ball light path system;4th, detecting system;5th, computer;6th, thoroughly
Mirror;7th, adapter;8th, support frame;9th, metal tube;10th, rice leaf tissue sample.
Embodiment
As shown in figure 1, the measurement apparatus of rice leaf tissue optical property parameter of the present utility model mainly includes:
The list that light-source system 1, the diffusing reflection rate to sample and the diffusing transmission rate for providing light source for experimental system are detected
Integrating sphere light path system 3, the optical signal in experiment switched to electric signal detecting system 4, data display and processing computer
5;Light-source system 1, simple integral ball light path system 3 and detecting system 4 are 380~1000nm by a diameter of 400 μm, wavelength band
Optical fiber 2 connect, detecting system 4 connection computer 5.
The power supply service tank group that the halogen tungsten lamp source and rated power that light-source system 1 is 250~2500nm by wave band are 250W
Into its light source power is adjustable, and intensity mode, current-mode or power mode, and the ginseng tested every time may be selected according to demand
Number setting can be stored, and experiment next time can be directly invoked during parameter setting;
Simple integral ball light path system 3 includes:
Integrating sphere, its inwall scribbles highly-reflective coating, and such as Spectraflect white coatings can be formed uniformly in inwall
Light intensity;
Hot spot adjuster, is 8mm by single lens 6 and/or internal diameter, and external diameter is 12mm, and length is 300mm metal tube 9
Composition, the outer surface of metal tube 9 scribble with integrating sphere inwall identical highly-reflective coating, lens are convex lens.
As shown in Fig. 2 when measuring the transmissivity of rice leaf tissue, hot spot adjuster is lens 6, passes through adapter 7
The specimen port of the integrating sphere side of optical fiber 2 is arranged close to, adapter 7 is arranged on integrating sphere by support frame 8.
As shown in figure 3, when measuring the reflectivity of rice leaf tissue, hot spot adjuster includes metal tube 9 and lens 6,
Metal tube 9 is extend into inside integrating sphere by the light inlet of the integrating sphere side away from specimen port, and metal tube 9 is consolidated by adapter 7
Fixed, adapter 7 is arranged on integrating sphere by support frame 8, and lens 6 are arranged on one end being located inside integrating sphere of metal tube 9.
Lens 6 and metal tube 9 are used for the hot spot for adjusting optical fiber 2, and it is 1mm to make to be radiated at the spot size on sample.
Specimen holder, including one end are fixed, and the moveable metal frame of the other end, the two ends of metal frame are fixed with quartz slide,
The moveable one end of metal frame can be adjusted according to the thickness of sample;
Support frame 8, for fixing optical fiber, metal tube and specimen holder;
Detecting system 4 includes:Wavelength band is 350~1000nm marine optics detector, and is located at ocean light
Learn the translucent cover outside detector;
Computer 5, is analyzed and processed for data acquisition, and to the data collected, is realized to rice leaf tissue
Optical property parameter is reconstructed.
As shown in figure 4, as follows to the inversion method of rice leaf tissue optical property parameter based on above-mentioned measurement apparatus:
(1) optical property parameter simulated data sets (μ is seta, μs), wherein μaFor absorption coefficient, μaFrom 0.01cm-1Start
With 1cm-1Step-length increase to 20cm-1, totally 20 data;μsFor scattering coefficient, μsFrom 1cm-1Start with 33cm-1Step-length increase
Add to 1000cm-1, totally 30 data form 20 × 30 totally 600 groups of data, it is 1 to set anisotropy factor g;Utilize Meng Teka
Sieve simulation program, obtains diffusing reflection rate, the diffusing transmission rate (R of the corresponding sample of the simulated data setsd, Td), wherein, RdIt is anti-to overflow
Penetrate rate, TdFor diffusing transmission rate.600 groups of (μa, μs) and corresponding (Rd, Td) constitute tranining database;
(2) configuration has fixed optical property parameter (μa', μs') and g one group of standard imitate body, standard of the present utility model
Imitative body is the solution of fat emulsion and dyestuff.Its corresponding diffusing reflection rate is obtained using measurement apparatus of the present utility model and unrestrained saturating
Penetrate rate (Rd', Td'), it is compared with the analog quantity in tranining database, tranining database is corrected;
(3) build initialization BP neural network, including input layer, the first hidden layer, the second hidden layer and output layer, input layer and
Output layer has 600 nodes, and the first hidden layer and the second hidden layer have 25 nodes, the transmission of the first hidden layer and the second hidden layer
" tansing " function and " purelin " function is respectively adopted in function, and learning function uses " learngdm " function, network training
Middle to use " traingd " function, performance function " mse " evaluates the performance of BP neural network with mean square error.
The input signal of input layer is (Rd, Td), i.e. the diffusing reflection rate of sample and diffusing transmission rate, the output signal of output layer
For (μa, μs), i.e. the optical property parameter absorption coefficient and scattering coefficient of sample;
The BP neural network of foundation is trained using the tranining database after correction, until error of fitting is less than set
The threshold value put, the threshold value can be set according to actual needs;BP neural network after being trained;
What BP neural network was trained concretely comprises the following steps:
1) tranining database that DSMC is set up is used for the training signal of neutral net error signal.Determine defeated
Enter the input of each unit in signal, connection weight and threshold calculations hidden layer, then calculated with the input of each unit by transmission function
The output of each unit in hidden layer;
2) using the input of the output of hidden layer, connection weight and threshold calculations output layer each unit, transmission function is then utilized
Calculate the corresponding output of output layer each unit;
3) using theoretical output, the reality output of network calculates the vague generalization error of each unit of output layer;
4) using connection weight, the vague generalization error of output layer and the output of hidden layer, the vague generalization error of hidden layer is calculated;
5) connection weight and threshold value are corrected using the output of the vague generalization error and hidden layer of output layer each unit.
(4) measurement parameter (R of rice leaf tissue to be measured is measured using measurement apparatus of the present utility modeld, Td), as
BP neural network after input quantity input training, BP neural network output after training relative to rice leaf tissue to be measured
Optical property parameter (μa, μs), it is achieved thereby that the anti-structure of the optical property parameter to rice leaf tissue.
The technical solution of the utility model and beneficial effect are described in detail embodiment described above, it should be understood that
Be that the foregoing is only specific embodiment of the utility model, be not limited to the utility model, it is all in the utility model
Spirit in any modification, supplement and the equivalent substitution made etc., should be included in protection domain of the present utility model it
It is interior.
Claims (6)
1. a kind of measurement apparatus of rice leaf tissue optical property parameter, including the light-source system, the list that are connected by optical fiber are long-pending
Bulb separation light path system and detecting system, detecting system connection computer, it is characterised in that
Described light-source system includes:Wave band is 250~2500nm halogen tungsten lamp source;
Described simple integral ball light path system includes:
Integrating sphere;
Hot spot adjuster, on integrating sphere, including metal tube and/or lens, the spot size for controlling optical fiber.
2. the measurement apparatus of rice leaf tissue optical property parameter according to claim 1, it is characterised in that work as measurement
During the transmissivity of rice leaf tissue, described hot spot adjuster is lens, is arranged close to the sample of the integrating sphere side of optical fiber
This mouth.
3. the measurement apparatus of rice leaf tissue optical property parameter according to claim 1, it is characterised in that work as measurement
During the reflectivity of rice leaf tissue, described hot spot adjuster includes metal tube and lens, and described metal tube passes through remote
The light inlet of the integrating sphere side of specimen port is extend into inside integrating sphere, and described lens are located at integrating sphere installed in metal tube
Internal one end.
4. the measurement apparatus of the rice leaf tissue optical property parameter according to claim 1 or 3, it is characterised in that institute
The internal diameter for the metal tube stated is 8mm, external diameter 12mm, and the length of metal tube is 300mm.
5. the measurement apparatus of rice leaf tissue optical property parameter according to claim 4, it is characterised in that described
The outer surface of metal tube scribbles highly-reflective coating.
6. the measurement apparatus of rice leaf tissue optical property parameter according to claim 1, it is characterised in that described
The translucent cover that detecting system includes detection spectrometer and covered on outside detection spectrometer.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106770047A (en) * | 2017-01-09 | 2017-05-31 | 浙江大学 | The inversion method and measurement apparatus of a kind of rice leaf tissue optical property parameter |
CN110596098A (en) * | 2019-08-26 | 2019-12-20 | 江苏大学 | Rapid detection system and detection method for ratio distribution of chlorophyll and lutein in cucumber leaves |
-
2017
- 2017-01-09 CN CN201720021180.0U patent/CN206431046U/en not_active Expired - Fee Related
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106770047A (en) * | 2017-01-09 | 2017-05-31 | 浙江大学 | The inversion method and measurement apparatus of a kind of rice leaf tissue optical property parameter |
CN110596098A (en) * | 2019-08-26 | 2019-12-20 | 江苏大学 | Rapid detection system and detection method for ratio distribution of chlorophyll and lutein in cucumber leaves |
CN110596098B (en) * | 2019-08-26 | 2022-05-20 | 江苏大学 | Rapid detection system and detection method for ratio distribution of chlorophyll and lutein in cucumber leaves |
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