CN214571994U - Small-size multi-functional cell culture jar - Google Patents

Abstract

The utility model discloses a small-size multi-functional cell culture jar, which comprises a tank body, jar body top is provided with servo motor, servo motor output shaft end transmission is connected with the (mixing) shaft, jar body top is connected with the connecting plate, servo motor is fixed to be set up at the connecting plate top, the fixed stirring rake that is provided with of (mixing) shaft outer peripheral face, jar body top one side is run through and is set up deep intake pipe, fixed filter and the solenoid valve of being provided with in the deep intake pipe. The utility model discloses a stirring rake rotates and realizes stirring the culture solution, through pressing from both sides the internal temperature of cover control jar, and the filter filters the bacterium in the gas, observes the height of the internal liquid level of jar through two rectangular sight glasses, and the level gauge is inserted the liquid level of the internal liquid of detection jar in the sample connection simultaneously to realize the internal liquid level condition of real-time observation jar.

Description

Small-size multi-functional cell culture jar

Technical Field

The utility model relates to a cell culture technical field, concretely relates to small-size multi-functional cell culture jar.

Background

Cell culture (cell culture) refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro to enable the cells to survive, grow and reproduce and maintain main structures and functions. Cell culture techniques are widely used in the fields of cytology, genetics, immunology, experimental medicine, oncology, and the like.

At present, animal cell culture usually requires a cell culture tank or the like. However, the existing cell culture tanks have more or less some defects, such as: most of the existing cell culture tanks can not realize temperature control on the cell culture tanks and can not provide comfortable temperature environment for cells; most of the existing cell culture tanks directly introduce oxygen or carbon dioxide into a culture tube, and unfiltered oxygen or carbon dioxide contains more bacteria, so that cells are easy to die; most of the existing cell culture tanks are closed, so that the liquid level height in the culture tank is inconvenient to observe, and the condition in the culture tank is inconvenient to observe in real time.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a small-size multi-functional cell culture jar rotates through the stirring rake and realizes the culture solution stirring, through pressing from both sides cover control tank internal temperature, the filter filters the bacterium in with gas, observes the height of the internal liquid level of jar through two rectangular sight glasses, inserts the level gauge simultaneously to the liquid level of the internal liquid of sample cell internal detection jar in the sample connection to realize the internal liquid level condition of real-time observation jar, with the problem among the above-mentioned background art of solution.

In order to achieve the above object, the present invention provides the following technical solutions: the utility model provides a small-size multi-functional cell culture jar, includes a jar body, jar body top is provided with servo motor, servo motor output shaft end transmission is connected with the (mixing) shaft, jar body top is connected with the connecting plate, servo motor is fixed to be set up at the connecting plate top, the fixed stirring rake that is provided with of (mixing) shaft outer peripheral face, jar body top one side is run through and is set up deep intake pipe, fixed filter and the solenoid valve of being provided with in the deep intake pipe, the fixed baffle that is provided with of jar internal wall, jar fixed the being provided with in bottom presss from both sides the cover, the fixed two rectangular sight glasses that are provided with of jar external peripheral face.

Preferably, the stirring rake is provided with two sets ofly, every group the stirring rake is all fixed to be provided with two, two of every group the stirring rake all is the axisymmetric setting about the (mixing) shaft is vertical to the central axis.

Preferably, a manhole is fixedly arranged on one side, far away from the deep-layer air inlet pipe, of the top of the tank body.

Preferably, the bottom of the servo motor is provided with a magnetic coupling sealing element, and the stirring shaft is connected with the tank cover in a sealing manner through the magnetic coupling sealing element.

Preferably, one side of the top of the tank cover, which is close to the deep-layer air inlet pipe, is provided with a liquid inlet pipe in a penetrating manner, and the bottom of the liquid inlet pipe is connected with a spraying ball.

Preferably, an illuminating vision lamp is fixedly arranged on one side, close to the deep-layer air inlet pipe, of the top of the tank body.

Preferably, the outer peripheral surface of the tank body is fixedly provided with a heat preservation layer, and the two strip sight glasses are embedded into the heat preservation layer.

Preferably, the bottom of the tank body is connected with a tank bottom valve, and one side of the bottom of the tank body, which is close to the deep-layer air inlet pipe, is fixedly provided with a standby port.

Preferably, a material supplementing pipe is fixedly arranged on one side, far away from the deep-layer air inlet pipe, of the top of the tank body, and a sampling port is fixedly arranged on one side, far away from the deep-layer air inlet pipe, of the bottom of the tank body.

In the technical scheme, the utility model provides a technological effect and advantage: the stirring shaft and the stirring paddle are driven to rotate by the servo motor, the stirring paddle rotates to stir the culture solution, the baffle plate enables the culture solution to impact and flow back, thereby fully mixing the cells and the culture solution, leading the culture effect of the cells to be better, controlling the temperature in the tank by introducing water and steam into the jacket, stirring the culture solution by the stirring paddle, leading the culture solution to be heated uniformly, giving a comfortable culture environment to the cells, promoting the cell culture, introducing gases such as oxygen, carbon dioxide and the like into the tank body through the deep-layer air inlet pipe, controlling the start and stop of the introduction of the gases by an electromagnetic valve, filtering the introduced gases by a filter to filter bacteria in the gases so as to avoid cell death caused by the bacteria, observing the height of the liquid level in the tank body through two strip sight glasses, simultaneously, the liquid level meter is inserted into the sampling port to detect the liquid level of the liquid in the tank body, so that the liquid level condition in the tank body can be observed in real time.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings can be obtained by those skilled in the art according to these drawings.

Fig. 1 is a schematic view of the overall structure of the present invention.

Fig. 2 is an enlarged view of a portion a of fig. 1 according to the present invention.

Description of reference numerals:

1. a tank body; 2. a servo motor; 3. a stirring shaft; 4. a connecting plate; 5. a stirring paddle; 6. a deep layer air inlet pipe; 7. a filter; 8. an electromagnetic valve; 9. a baffle plate; 10. a jacket; 11. a strip sight glass; 12. a manhole; 13. a magnetic coupling seal; 14. a liquid inlet pipe; 15. spraying a ball; 16. an illuminated vision light; 17. a heat-insulating layer; 18. a tank bottom valve; 19. a spare port; 20. a material supplementing pipe; 21. a sampling port.

Detailed Description

Example embodiments will now be described more fully with reference to the accompanying drawings. Example embodiments may, however, be embodied in many different forms and should not be construed as limited to the examples set forth herein; rather, these example embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of example embodiments to those skilled in the art. The drawings are merely schematic illustrations of the present disclosure and are not necessarily drawn to scale. The same reference numerals in the drawings denote the same or similar parts, and thus their repetitive description will be omitted.

Furthermore, the described features, structures, or characteristics may be combined in any suitable manner in one or more example embodiments. In the following description, numerous specific details are provided to give a thorough understanding of example embodiments of the disclosure. One skilled in the relevant art will recognize, however, that the subject matter of the present disclosure can be practiced without one or more of the specific details, or with other methods, components, steps, and so forth. In other instances, well-known structures, methods, implementations, or operations are not shown or described in detail to avoid obscuring aspects of the disclosure.

The utility model provides a small-size multi-functional cell culture jar as shown in fig. 1-2, which comprises a tank body 1, 1 top of the jar body is provided with servo motor 2, 2 output shaft end transmission of servo motor are connected with (mixing) shaft 3, 1 top of the jar body is connected with connecting plate 4, 2 fixed settings at connecting plate 4 tops of servo motor, the fixed stirring rake 5 that is provided with of 3 outer peripheral faces of (mixing) shaft, 1 top one side of the jar body is run through and is set up deep intake pipe 6, fixed filter 7 and the solenoid valve 8 of being provided with in deep intake pipe 6, the fixed baffle 9 that is provided with of 1 inner wall of the jar body, the fixed clamp cover 10 that is provided with in 1 bottom of the jar body, the fixed two rectangular sight glasses 11 that are provided with in 1 outer peripheral face of the jar body.

Further, in the above technical scheme, stirring rake 5 is provided with two sets ofly, every group stirring rake 5 is all fixed and is provided with two, two of every group stirring rake 5 is all vertical to the central axis about (mixing) shaft 3 and is the axisymmetric setting, realizes the stirring to the culture solution.

Further, in the above technical scheme, a manhole 12 is fixedly arranged on one side of the top of the tank body 1 far away from the deep-layer air inlet pipe 6, so that feeding is facilitated.

Further, in the above technical solution, a magnetic coupling seal 13 is disposed at the bottom of the servo motor 2, and the stirring shaft 3 is hermetically connected to the tank cover through the magnetic coupling seal 13.

Further, in the above technical scheme, a liquid inlet pipe 14 is penetratingly arranged at one side of the top of the tank cover close to the deep-layer air inlet pipe 6, and a spraying ball 15 is connected to the bottom of the liquid inlet pipe 14, so that the tank body 1 can be conveniently cleaned.

Further, in the above technical scheme, an illumination lamp 16 is fixedly arranged on one side of the top of the tank body 1 close to the deep-layer air inlet pipe 6, so that the tank body 1 is illuminated, and the liquid level in the tank body 1 can be observed conveniently.

Further, in above-mentioned technical scheme, jar body 1 outer peripheral face is fixed and is provided with heat preservation 17, two rectangular sight glass 11 all imbeds to heat preservation 17 in, realizes keeping warm, avoids the heat to run off in a large number.

Further, in the above technical scheme, tank bottom valve 18 is connected to the bottom of the tank body 1, the discharge of materials is facilitated, and a standby port 19 is fixedly arranged on one side of the bottom of the tank body 1, which is close to the deep layer air inlet pipe 6, and is convenient for standby.

Further, in above-mentioned technical scheme, the fixed material pipe 20 that is provided with of one side that keeps away from deep intake pipe 6 at jar body 1 top, the feed supplement of being convenient for, the fixed sample connection 21 that is provided with of one side that keeps away from deep intake pipe 6 in jar body 1 bottom is convenient for take a sample, the level gauge of being convenient for simultaneously is to the liquid level detection in jar body 1.

The implementation mode is specifically as follows: during the actual use, pass through the wire with servo motor 2 and solenoid valve 8 and external power supply switch on, add culture solution and cell in jar body 1 through manhole 12, switch on servo motor 2 drives (mixing) shaft 3 and stirring rake 5 and rotates, stirring rake 5 rotates and realizes stirring to the culture solution, baffle 9 makes the culture solution striking backward flow, thereby make cell and culture solution intensive mixing, make the culture effect of cell better, control jar internal temperature through letting in water and steam in pressing from both sides cover 10, stirring rake 5 stirs the culture solution thereby make the culture solution be heated evenly, give the comfortable culture environment of cell, promote cell culture, let in gas such as oxygen, gas such as carbon dioxide through deep intake pipe 6 in jar body 1, the start-stop of opening of gas is controlled by solenoid valve 8, the gas that lets in all filters through filter 7, filter the bacterium in the gas, avoid the bacterium to cause cell death, observe the height of the interior liquid level of jar body 1 through two rectangular sight glasses 11, simultaneously with the level gauge ann insert the liquid level of the interior liquid of the detection jar body 1 of sample connection 21, thereby realize the liquid level condition in the real-time observation jar body 1, this embodiment has specifically solved the current cell culture jar that exists among the prior art and mostly can not realize the accuse temperature to the cell culture jar, can not give the comfortable temperature environment of cell, the current cell culture jar is mostly directly let in oxygen or carbon dioxide to the culture tube in, because not filterable oxygen or carbon dioxide contain more bacterium, lead to cell death easily and the current cell culture jar mostly be the liquid level height in the inconvenient observation culture jar of closed, thereby the problem of the inconvenient real-time observation culture jar interior condition.

This practical theory of operation: rotate through stirring rake 5 and realize stirring the culture solution, through pressing from both sides the interior temperature of cover 10 control tank, filter 7 filters the bacterium in the gas, observes the height of the interior liquid level of the jar body 1 through two rectangular sight glasses 11, inserts the level gauge simultaneously to the liquid level of the interior liquid of the detection jar body 1 of sample connection 21 to realize the liquid level condition in the real-time observation jar body 1.

While certain exemplary embodiments of the present invention have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that the described embodiments may be modified in various different ways without departing from the spirit and scope of the present invention. Accordingly, the drawings and description are illustrative in nature and should not be construed as limiting the scope of the invention.

Claims (9)

1. The utility model provides a small-size multi-functional cell culture jar, includes jar body (1), its characterized in that: the utility model discloses a multi-functional jar, including jar body (1), servo motor (2) output shaft end transmission is connected with (mixing) shaft (3), jar body (1) top is connected with connecting plate (4), servo motor (2) are fixed to be set up at connecting plate (4) top, the fixed stirring rake (5) that is provided with of (mixing) shaft (3) outer peripheral face, jar body (1) top one side is run through and is set up deep intake pipe (6), fixed filter (7) and solenoid valve (8) of being provided with on deep intake pipe (6), jar body (1) inner wall is fixed and is provided with baffle (9), jar body (1) bottom is fixed to be provided with and presss from both sides cover (10), jar body (1) outer peripheral face is fixed and is provided with two rectangular sight glass (11).

2. The small scale multipurpose cell culture tank of claim 1, wherein: stirring rake (5) are provided with two sets ofly, every group stirring rake (5) are all fixed to be provided with two, two of every group stirring rake (5) are all about (mixing) shaft (3) vertical to the central axis be the axisymmetric setting.

3. The small scale multipurpose cell culture tank of claim 1, wherein: a manhole (12) is fixedly arranged on one side, far away from the deep air inlet pipe (6), of the top of the tank body (1).

4. The multifunctional small cell culture pot according to claim 1, wherein: the bottom of the servo motor (2) is provided with a magnetic coupling sealing piece (13), and the stirring shaft (3) is connected with the tank cover in a sealing mode through the magnetic coupling sealing piece (13).

5. The small scale multifunctional cell culture tank according to claim 4, wherein: one side of the top of the tank cover, which is close to the deep-layer air inlet pipe (6), is provided with a liquid inlet pipe (14) in a penetrating way, and the bottom of the liquid inlet pipe (14) is connected with a spraying ball (15).

6. The small scale multipurpose cell culture tank of claim 1, wherein: an illuminating lamp (16) is fixedly arranged on one side of the top of the tank body (1) close to the deep-layer air inlet pipe (6).

7. The small scale multipurpose cell culture tank of claim 1, wherein: jar body (1) outer peripheral face is fixed and is provided with heat preservation (17), two rectangular sight glass (11) all imbeds in to heat preservation (17).

8. The small scale multipurpose cell culture tank of claim 1, wherein: the bottom of the tank body (1) is connected with a tank bottom valve (18), and one side of the bottom of the tank body (1) close to the deep-layer air inlet pipe (6) is fixedly provided with a standby port (19).

9. The small scale multipurpose cell culture tank of claim 1, wherein: one side of keeping away from deep intake pipe (6) at jar body (1) top is fixed to be provided with material supplementing pipe (20), one side of keeping away from deep intake pipe (6) at jar body (1) bottom is fixed to be provided with sample connection (21).

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