CN214361440U - Stainless steel cell culture jar - Google Patents
Stainless steel cell culture jar Download PDFInfo
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- CN214361440U CN214361440U CN202120195398.4U CN202120195398U CN214361440U CN 214361440 U CN214361440 U CN 214361440U CN 202120195398 U CN202120195398 U CN 202120195398U CN 214361440 U CN214361440 U CN 214361440U
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- tank
- cell culture
- cover
- stainless steel
- servo motor
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Abstract
The utility model discloses a stainless steel cell culture jar, which comprises a tank body, the fixed clamp cover that is provided with in jar body bottom, jar body top is provided with the cover, cover top central point puts the fixed servo motor that is provided with, servo motor output shaft end transmission is connected with the (mixing) shaft, the servo motor bottom is provided with the magnetic coupling sealing member, the (mixing) shaft passes through magnetic coupling sealing member and cover sealing connection. The utility model discloses a servo motor drives the stirring rake and rotates, lets in water and steam to controlling the tank interior temperature in pressing from both sides the cover, thereby the stirring rake makes the culture solution be heated evenly to the culture solution stirring, gives the comfortable cultivation environment of cell, promotes cell culture, lets in gas such as oxygen, carbon dioxide through deep intake pipe to the jar internal, gaseous letting in, stopping by solenoid valve control, the gas that lets in all filters through the filter, filters the bacterium in the gas, avoids the bacterium to cause the cell death.
Description
Technical Field
The utility model relates to a cell culture technical field, concretely relates to stainless steel cell culture jar.
Background
Cells are not uniformly defined, and the more common method is: the cell is the basic structure and function unit of organism, all organisms except the virus are known to be composed of the cell, but the life activity of the virus can be embodied only in the cell, generally speaking, most microorganisms such as bacteria and the like and protozoa are composed of one cell, namely, unicellular organisms, higher plants and higher animals are multicellular organisms, the cell can be divided into two types of prokaryotic cell and eukaryotic cell, but also can be divided into three types according to the proposal, namely, ancient nuclear cells originally belonging to the prokaryotic cell are independently taken as parallel type with the ancient nuclear cells, the cell body is extremely tiny, can be observed under a microscope, has various shapes, mainly comprises cell nucleus and cytoplasm, has cell membrane on the surface, has cell wall outside the plant cell membrane, has plastid in the cytoplasm, has chloroplast and vacuole in vivo, also has mitochondria, and has no cell wall of the animal cell, the cytoplasm is usually provided with a centrosome, but higher plant cells are not provided with the centrosome, the cells have the functions of movement, nutrition, propagation and the like, and a special cell culture tank is needed during cell culture.
The prior art has the following defects: most of the existing cell culture tanks cannot realize temperature control of the cell culture tanks and cannot provide a comfortable temperature environment for cells, and most of the existing cell culture tanks directly introduce oxygen or carbon dioxide into culture tubes, and because unfiltered oxygen or carbon dioxide contains more bacteria, cell death is easily caused.
The above information disclosed in this background section is only for enhancement of understanding of the background of the disclosure and therefore it may contain information that does not constitute prior art that is already known to a person of ordinary skill in the art.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a stainless steel cell culture jar, it rotates to drive the stirring rake through servo motor, to letting in water and steam in the cover of clamp and control the tank inside temperature, thereby the stirring rake makes the culture solution be heated evenly to the culture solution stirring, give the comfortable cultivation environment of cell, promote cell culture, to the internal oxygen that lets in of jar through deep intake pipe, gas such as carbon dioxide, gaseous letting in, stop by solenoid valve control, the gas that lets in all filters through the filter, with the bacterial filtration in the gas, avoid the bacterium to cause cell death, with the problem in solving above-mentioned background art.
In order to achieve the above object, the present invention provides the following technical solutions: the utility model provides a stainless steel cell culture jar, which comprises a tank body, the fixed cover that presss from both sides that is provided with in jar body bottom, jar body top is provided with the cover, cover top central point puts the fixed servo motor that is provided with, servo motor output shaft end transmission is connected with the (mixing) shaft, the servo motor bottom is provided with the magnetic coupling sealing member, the (mixing) shaft passes through magnetic coupling sealing member and cover sealing connection, the (mixing) shaft outer peripheral face is connected with the stirring rake, jar body top one side is run through and is set up deep intake pipe, fixed filter and the solenoid valve of being provided with in the deep intake pipe, the fixed a plurality of sight glasses of taking that are provided with in cover top, just the fixed pressure sensor mouth and the condenser interface that are provided with in cover top.
Preferably, the stirring rake is provided with two sets ofly, every group the stirring rake is all fixed to be provided with two, two of every group the stirring rake all is the axisymmetric setting about the (mixing) shaft is vertical to the central axis.
Preferably, the top of the tank cover is connected with a spraying ball in a penetrating manner, and a feeding hole is fixedly formed in one side, far away from the spraying ball, of the top of the tank cover.
Preferably, the top of the outer peripheral surface of the tank body is connected with an upper flange, a plurality of fasteners are connected between the tank cover and the upper flange, and the included angle between any two adjacent fasteners is 60 degrees.
Preferably, a material supplementing opening is fixedly arranged on one side, far away from the spraying ball, of the top of the tank body.
Preferably, the bottom of the tank body is connected with a tank bottom valve, and one side of the bottom of the tank body, which is far away from the spraying ball, is connected with a sampling valve.
Preferably, the bottom of the jacket is connected with a liquid inlet pipe.
Preferably, the outer peripheral surface of the tank body is fixedly provided with two strip sight glasses, and the top of the tank cover is fixedly provided with a lifting device.
In the technical scheme, the utility model provides a technological effect and advantage:
through feed inlet to jar internal addition culture solution and cell, servo motor drives (mixing) shaft and stirring rake and rotates, the stirring rake rotates and realizes stirring the cell, let in water and steam to pressing from both sides the cover through the feed liquor pipe and control the tank interior temperature, thereby the stirring rake makes the culture solution be heated evenly to the culture solution stirring, give the comfortable cultivation environment of cell, promote cell culture, let in oxygen to jar internal through deep intake pipe, gas such as carbon dioxide, gaseous letting in, stop by solenoid valve control, the gas that lets in all filters through the filter, with the bacterial filtration in the gas, avoid the bacterium to cause cell death.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings can be obtained by those skilled in the art according to these drawings.
Fig. 1 is a cross-sectional view of the overall structure of the present invention.
Fig. 2 is a partial structure front view of the tank body of the present invention.
Fig. 3 is a partial top view of the can lid of the present invention.
Description of reference numerals:
1. a tank body; 2. a jacket; 3. a can lid; 4. a servo motor; 5. a stirring shaft; 6. a magnetic coupling seal; 7. a stirring paddle; 8. a deep layer air inlet pipe; 9. a filter; 10. an electromagnetic valve; 11. a sight glass with a lamp; 12. a pressure sensor port; 13. a condenser interface; 14. spraying a ball; 15. an upper flange; 16. a fastener; 17. a material supplementing port; 18. a tank bottom valve; 19. a sampling valve; 20. a liquid inlet pipe; 21. a strip sight glass; 22. a lifting device.
Detailed Description
Example embodiments will now be described more fully with reference to the accompanying drawings. Example embodiments may, however, be embodied in many different forms and should not be construed as limited to the examples set forth herein; rather, these example embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of example embodiments to those skilled in the art. The drawings are merely schematic illustrations of the present disclosure and are not necessarily drawn to scale. The same reference numerals in the drawings denote the same or similar parts, and thus their repetitive description will be omitted.
Furthermore, the described features, structures, or characteristics may be combined in any suitable manner in one or more example embodiments. In the following description, numerous specific details are provided to give a thorough understanding of example embodiments of the disclosure. One skilled in the relevant art will recognize, however, that the subject matter of the present disclosure can be practiced without one or more of the specific details, or with other methods, components, steps, and so forth. In other instances, well-known structures, methods, implementations, or operations are not shown or described in detail to avoid obscuring aspects of the disclosure.
The utility model provides a stainless steel cell culture tank as shown in figures 1-3, which comprises a tank body 1, a jacket 2 is fixedly arranged at the bottom of the tank body 1, a tank cover 3 is arranged at the top of the tank body 1, a servo motor 4 is fixedly arranged at the center of the top of the tank cover 3, the end part of the output shaft of the servo motor 4 is connected with a stirring shaft 5 in a transmission way, the bottom of the servo motor 4 is provided with a magnetic coupling sealing element 6, the stirring shaft 5 is hermetically connected with the tank cover 3 through a magnetic coupling sealing piece 6, the peripheral surface of the stirring shaft 5 is connected with a stirring paddle 7, a deep air inlet pipe 8 is arranged on one side of the top of the tank body 1 in a penetrating way, a filter 9 and an electromagnetic valve 10 are fixedly arranged on the deep air inlet pipe 8, a plurality of sight glasses 11 with lamps are fixedly arranged on the top of the tank cover 3, and a pressure sensor port 12 and a condenser port 13 are fixedly arranged at the top of the tank cover 3.
Further, in above-mentioned technical scheme, stirring rake 7 is provided with two sets ofly, every group stirring rake 7 is all fixed to be provided with two, two of every group stirring rake 7 is all about 5 vertical to the central axis be the axisymmetric setting of (mixing) shaft, realizes stirring the culture solution for the culture solution is heated evenly.
Further, in the above technical scheme, the top of the tank cover 3 is connected with the spraying ball 14 in a penetrating manner, and a feeding hole is fixedly formed in one side of the top of the tank cover 3, which is far away from the spraying ball 14, so that the tank body 1 can be cleaned conveniently.
Further, in the above technical solution, the top of the outer circumferential surface of the tank body 1 is connected with an upper flange 15, a plurality of fasteners 16 are connected between the tank cover 3 and the upper flange 15, and an included angle between any two adjacent fasteners 16 is 60 degrees.
Further, in the above technical scheme, a material supplementing port 17 is fixedly arranged on one side of the top of the tank body 1 far away from the spraying ball 14, so that material supplementing is facilitated.
Preferably, the bottom of the tank body 1 is connected with a tank bottom valve 18, and one side of the bottom of the tank body 1, which is far away from the spraying ball 14, is connected with a sampling valve 19, so that sampling is facilitated.
Further, in the above technical solution, the bottom of the jacket 2 is connected with a liquid inlet pipe 20, which is convenient for introducing water and steam into the jacket 2.
Furthermore, in the above technical solution, two strip view mirrors 21 are fixedly arranged on the outer peripheral surface of the tank body 1, and a lifting device 22 is fixedly arranged on the top of the tank cover 3, so as to facilitate lifting of the tank cover 3.
The implementation mode is specifically as follows: during practical use, the servo motor 4 and the electromagnetic valve 10 are connected with an external power supply through wires, culture solution and cells are added into the tank body 1 through the feed inlet, the servo motor 4 is connected to drive the stirring shaft 5 and the stirring paddle 7 to rotate, the stirring paddle 7 rotates to stir the cells, water and steam are introduced into the jacket 2 through the liquid inlet pipe 20 to control the temperature in the tank, the stirring paddle 7 stirs the culture solution to enable the culture solution to be heated uniformly, a comfortable culture environment is provided for the cells, cell culture is promoted, gases such as oxygen, carbon dioxide and the like are introduced into the tank body 1 through the deep air inlet pipe 8, the introduction and stopping of the gases are controlled by the electromagnetic valve 10, the introduced gases are filtered through the filter 9, bacteria in the gases are filtered, cell death caused by the bacteria is avoided, and the embodiment specifically solves the problem that most of the existing cell culture tank in the prior art can not realize temperature control of the cell culture tank, the cell culture tank cannot provide a comfortable temperature environment for cells, most of the existing cell culture tanks directly introduce oxygen or carbon dioxide into a culture tube, and unfiltered oxygen or carbon dioxide contains more bacteria, so that the cell death is easily caused.
This practical theory of operation: drive stirring rake 7 through servo motor 4 and rotate, to letting in water and steam in pressing from both sides cover 2 and control the tank interior temperature, thereby stirring rake 7 makes the culture solution be heated evenly to the culture solution stirring, give the comfortable cultivation environment of cell, promote cell culture, let in oxygen, gas such as carbon dioxide in to jar body 1 through deep intake pipe 8, gaseous the letting in, stop by solenoid valve 10 control, the gas that lets in all filters through filter 9, with the bacterial filtration in the gas, avoid the bacterium to cause cell death.
While certain exemplary embodiments of the present invention have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that the described embodiments may be modified in various different ways without departing from the spirit and scope of the present invention. Accordingly, the drawings and description are illustrative in nature and should not be construed as limiting the scope of the invention.
Claims (8)
1. The utility model provides a stainless steel cell culture jar, includes jar body (1), its characterized in that: a jacket (2) is fixedly arranged at the bottom of the tank body (1), a tank cover (3) is arranged at the top of the tank body (1), a servo motor (4) is fixedly arranged at the center of the top of the tank cover (3), the end part of an output shaft of the servo motor (4) is connected with a stirring shaft (5) in a transmission way, the bottom of the servo motor (4) is provided with a magnetic coupling sealing piece (6), the stirring shaft (5) is hermetically connected with the tank cover (3) through the magnetic coupling sealing piece (6), the outer peripheral surface of the stirring shaft (5) is connected with a stirring paddle (7), one side of the top of the tank body (1) is provided with a deep air inlet pipe (8) in a penetrating way, a filter (9) and an electromagnetic valve (10) are fixedly arranged on the deep layer air inlet pipe (8), a plurality of sight glasses (11) with lamps are fixedly arranged at the top of the tank cover (3), and a pressure sensor port (12) and a condenser interface (13) are fixedly arranged at the top of the tank cover (3).
2. A stainless steel cell culture tank according to claim 1, wherein: stirring rake (7) are provided with two sets ofly, every group stirring rake (7) are all fixed to be provided with two, two of every group stirring rake (7) are all vertical to the central axis about (mixing) shaft (5) and are the axisymmetric setting.
3. A stainless steel cell culture tank according to claim 1, wherein: the spraying device is characterized in that a spraying ball (14) is connected to the top of the tank cover (3) in a penetrating mode, and a feeding hole is fixedly formed in one side, far away from the spraying ball (14), of the top of the tank cover (3).
4. A stainless steel cell culture tank according to claim 1, wherein: the tank is characterized in that an upper flange (15) is connected to the top of the outer peripheral surface of the tank body (1), a plurality of fasteners (16) are connected between the tank cover (3) and the upper flange (15), and the included angle between any two adjacent fasteners (16) is 60 degrees.
5. A stainless steel cell culture tank according to claim 3, wherein: and a material supplementing opening (17) is fixedly arranged on one side of the top of the tank body (1) far away from the spraying ball (14).
6. A stainless steel cell culture tank according to claim 3, wherein: the bottom of the tank body (1) is connected with a tank bottom valve (18), and one side of the bottom of the tank body (1) far away from the spray ball (14) is connected with a sampling valve (19).
7. A stainless steel cell culture tank according to claim 1, wherein: the bottom of the jacket (2) is connected with a liquid inlet pipe (20).
8. A stainless steel cell culture tank according to claim 1, wherein: the outer peripheral surface of the tank body (1) is fixedly provided with two strip sight glasses (21), and the top of the tank cover (3) is fixedly provided with a lifting device (22).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120195398.4U CN214361440U (en) | 2021-01-25 | 2021-01-25 | Stainless steel cell culture jar |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120195398.4U CN214361440U (en) | 2021-01-25 | 2021-01-25 | Stainless steel cell culture jar |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214361440U true CN214361440U (en) | 2021-10-08 |
Family
ID=77958929
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120195398.4U Active CN214361440U (en) | 2021-01-25 | 2021-01-25 | Stainless steel cell culture jar |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214361440U (en) |
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2021
- 2021-01-25 CN CN202120195398.4U patent/CN214361440U/en active Active
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|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20221024 Address after: 226200 No.500, Linyang Road, Qidong Economic Development Zone, Nantong City, Jiangsu Province Patentee after: Wotisi (Nantong) Biotechnology Co.,Ltd. Address before: 215000 No. 16-1, south of Fenghuang Avenue, Fenghuang Town, Zhangjiagang City, Suzhou City, Jiangsu Province Patentee before: Suzhou womei biology Co.,Ltd. |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20221118 Address after: 215000 No. 16-1, south of Fenghuang Avenue, Fenghuang Town, Zhangjiagang City, Suzhou City, Jiangsu Province Patentee after: Suzhou womei biology Co.,Ltd. Address before: 226200 No.500, Linyang Road, Qidong Economic Development Zone, Nantong City, Jiangsu Province Patentee before: Wotisi (Nantong) Biotechnology Co.,Ltd. |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230117 Address after: 226200 No.500, Linyang Road, Qidong Economic Development Zone, Nantong City, Jiangsu Province Patentee after: Wotisi (Nantong) Biotechnology Co.,Ltd. Address before: 215000 No. 16-1, south of Fenghuang Avenue, Fenghuang Town, Zhangjiagang City, Suzhou City, Jiangsu Province Patentee before: Suzhou womei biology Co.,Ltd. |
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| TR01 | Transfer of patent right |