CN214270883U - Beauveria bassiana liquid fermentation tank - Google Patents
Beauveria bassiana liquid fermentation tank Download PDFInfo
- Publication number
- CN214270883U CN214270883U CN202120028396.6U CN202120028396U CN214270883U CN 214270883 U CN214270883 U CN 214270883U CN 202120028396 U CN202120028396 U CN 202120028396U CN 214270883 U CN214270883 U CN 214270883U
- Authority
- CN
- China
- Prior art keywords
- beauveria bassiana
- sampling
- metal
- jar
- metal bath
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 21
- 230000004151 fermentation Effects 0.000 title claims abstract description 21
- 239000007788 liquid Substances 0.000 title claims abstract description 17
- 241000751139 Beauveria bassiana Species 0.000 title claims abstract description 16
- 238000005070 sampling Methods 0.000 claims abstract description 46
- 229910052751 metal Inorganic materials 0.000 claims abstract description 30
- 239000002184 metal Substances 0.000 claims abstract description 30
- 230000001954 sterilising effect Effects 0.000 claims abstract description 21
- 238000004659 sterilization and disinfection Methods 0.000 claims description 12
- 229910018487 Ni—Cr Inorganic materials 0.000 claims description 3
- 239000000956 alloy Substances 0.000 claims description 3
- 229910045601 alloy Inorganic materials 0.000 claims description 3
- VNNRSPGTAMTISX-UHFFFAOYSA-N chromium nickel Chemical compound [Cr].[Ni] VNNRSPGTAMTISX-UHFFFAOYSA-N 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 abstract description 11
- 241000894006 Bacteria Species 0.000 abstract description 7
- 238000010438 heat treatment Methods 0.000 abstract description 5
- 241000233866 Fungi Species 0.000 abstract description 4
- 238000004134 energy conservation Methods 0.000 abstract description 2
- 238000009630 liquid culture Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000000967 entomopathogenic effect Effects 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a beauveria bassiana liquid fermentation cylinder, including a jar body, the internal top of jar is provided with metal bath sterilizing equipment, the inside a plurality of sheetmetals that are provided with of sterilizing equipment is bathed to the metal, and the sterilizing equipment both sides are bathed to the metal are connected with intake pipe and outlet duct respectively, the internal outer sampling pipe that sets up along jar body axial that still sets up of jar, the intraductal interior sampling pipe that is provided with of outer sampling, interior sampling pipe both ends all extend to jar external portion, are provided with interior sample connection on the interior sampling pipe, set up the outer sample connection of a plurality of equidistance along its axial on the outer sampling pipe. The utility model adopts the metal bath heating mode to heat and sterilize the air, on one hand, the external mixed bacteria pollution can be reduced, on the other hand, the hot air can keep the temperature of the culture medium constant, no extra heating is needed, and the energy conservation is facilitated; through the structure that sets up inside and outside sampling tube, not only can acquire the liquid sample of the different degree of depth of multiunit, also can reduce miscellaneous fungus and get into, and sample easy operation is swift.
Description
Technical Field
The utility model relates to a beauveria bassiana prepares relevant technical field, specifically is a beauveria bassiana liquid fermentation jar.
Background
Beauveria bassiana is one of the insecticidal fungi widely used for biological control of pests at home and abroad, and is considered to be an entomopathogenic fungus with the most development potential.
The beauveria bassiana is fermented and cultivated by adopting a liquid fermentation mode and a solid fermentation mode, wherein hyphae are cultivated by adopting a liquid culture medium in the liquid fermentation mode to obtain blastospores of the beauveria bassiana. Because the fluidity of the air in the fermentation tank needs to be maintained in the liquid culture process, foreign bacteria enter the fermentation tank in the air exchange process with the outside, and the quality of the bacteria is influenced, the existing fermentation tank adopts modes of ultraviolet sterilization and the like to sterilize the entering air, but on one hand, the sterilization is not thorough, and on the other hand, the energy consumption is high; in addition, the fermentation process needs to be regularly and repeatedly sampled and detected, on one hand, mixed bacteria can enter the fermentation process when a sampling opening is opened, on the other hand, both up-sampling and down-sampling can only sample samples near the sampling opening, and layered sampling cannot be carried out.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a beauveria bassiana liquid fermentation cylinder to solve the problem of proposing in the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme:
a fermentation tank for Beauveria bassiana liquid comprises a tank body, wherein a stirrer is arranged in the tank body, a metal bath sterilization device is arranged at the top in the tank body, the metal bath sterilization device is disc-shaped, a plurality of metal sheets are arranged in the metal bath sterilization device, the metal sheets are connected with a power supply, an air inlet pipe and an air outlet pipe are respectively connected to two sides of the metal bath sterilization device, the air inlet pipe is connected to the outside of the tank body, the air outlet pipe extends to the bottom in the tank body, and a plurality of air outlet holes are formed in the air outlet pipe;
the internal outer sample tube that still sets up along jar body axial setting of jar, outer sample intraductal interior sample tube that is provided with, interior sample tube both ends all extend to jar external portion, are provided with an interior sample connection on the interior sample tube, outer sample connection along its axial sets up the outer sample connection of a plurality of equidistance on the outer sample tube.
As a further aspect of the present invention: and the inner sampling pipe is provided with scales corresponding to the outer sampling ports.
As a further aspect of the present invention: and rubber rings are arranged on two sides of the outer sampling port.
As a further aspect of the present invention: the metal sheet is a nickel-chromium electrothermal alloy sheet.
As a further aspect of the present invention: a plurality of temperature sensors are arranged on the tank body.
Compared with the prior art, the beneficial effects of the utility model are that: the utility model adopts the metal bath heating mode to heat and sterilize the air, on one hand, the external mixed bacteria pollution can be reduced, on the other hand, the hot air can keep the temperature of the culture medium constant, no extra heating is needed, and the energy conservation is facilitated; through the structure that sets up inside and outside sampling tube, not only can acquire the liquid sample of the different degree of depth of multiunit, also can reduce miscellaneous fungus and get into, and sample easy operation is swift.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic structural view of the metal bath sterilization device of the present invention.
Fig. 3 is a schematic structural view of the outer sampling tube and the inner sampling tube of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1 and 2, in an embodiment of the present invention, a beauveria bassiana liquid fermentation tank includes a tank body 1, a stirrer 4 is disposed in the tank body 1, and the stirrer 4 is used for stirring a liquid culture medium in a fermentation process to improve a growth effect of mycelia; the metal bath sterilizing device 2 is arranged at the top in the tank body 1, the metal bath sterilizing device 2 is disc-shaped, a plurality of metal sheets 23 are arranged in the metal bath sterilizing device 2, the metal sheets 23 are nickel-chromium electrothermal alloy sheets, the metal sheets 23 are all connected with a power supply, an air inlet pipe 22 and an air outlet pipe 21 are respectively connected to two sides of the metal bath sterilizing device 2, the air inlet pipe 22 is connected to the outside of the tank body 1, the air outlet pipe 21 extends to the bottom in the tank body 1, a plurality of air outlet holes are formed in the air outlet pipe 21, the air inlet pipe 22 is used for blowing in external air, the air enters the metal bath sterilizing device 2, enters the air outlet pipe 21 after being heated by the metal sheets 23 and is blown out from the air outlet holes, the air is subjected to heating sterilization treatment in the metal bath sterilizing device 2, the blown air eliminates mixed bacteria, in addition, the heated air can heat the culture medium, thus the culture medium is kept in a certain temperature range, specifically, a plurality of temperature sensors 3 are arranged on the tank body 1 and used for detecting the temperature of the culture medium, according to the temperature values of the temperature sensors 3, when the temperature is higher than the threshold value of the set safe temperature range, air intake is stopped, when the temperature is lower than the safe temperature range after being slowly cooled, air is introduced, and the culture medium is heated by the blown air, so that the culture medium is kept in a certain safe temperature range;
as shown in fig. 3, an outer sampling tube 6 is further arranged in the tank body 1 along the axial direction of the tank body 1, an inner sampling tube 5 is arranged in the outer sampling tube 6, both ends of the inner sampling tube 5 extend to the outside of the tank body 1, an inner sampling port 51 is arranged on the inner sampling tube 5, a plurality of equidistant outer sampling ports 61 are arranged on the outer sampling tube 6 along the axial direction, the outer sampling ports 61 correspond to different depths of the culture medium in the tank body 1, during sampling, the inner sampling tube 5 is slowly drawn out from the bottom by pulling the bottom end of the inner sampling tube 5, during the drawing of the inner sampling tube 5, the upper inner sampling port 51 corresponds to each outer sampling port 61 from the top down, after the corresponding liquid flows out from the sampling ports, the sample at the depth position can be obtained, after the inner sampling port and the outer sampling port are separated, the sampling ports are sealed, and during next sampling, a plurality of culture medium samples can be obtained from the bottom to the top by pulling the upper end of the inner sampling tube 5, therefore, the sample can be determined according to the depth of the tank body, the problem that the sampling port is opened cannot occur in the process, and the mixed bacteria pollution is reduced.
The inner sampling pipe 5 is provided with scales corresponding to the outer sampling ports 61 to determine whether the positions of the inner and outer sampling ports correspond to each other.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (5)
1. The utility model provides a beauveria bassiana liquid state fermentation cylinder, includes jar body (1), set up agitator (4), its characterized in that in jar body (1): the metal bath sterilization device (2) is arranged at the top in the tank body (1), the metal bath sterilization device (2) is disc-shaped, a plurality of metal sheets (23) are arranged inside the metal bath sterilization device (2), the metal sheets (23) are all connected with a power supply, an air inlet pipe (22) and an air outlet pipe (21) are respectively connected to two sides of the metal bath sterilization device (2), the air inlet pipe (22) is connected to the outside of the tank body (1), the air outlet pipe (21) extends to the bottom in the tank body (1), and a plurality of air outlet holes are formed in the air outlet pipe (21);
still set up outer sample tube (6) along jar body (1) axial setting in jar body (1), be provided with interior sample tube (5) in outer sample tube (6), interior sample tube (5) both ends all extend to jar body (1) outside, are provided with one on interior sample tube (5) and take a sample mouthful (51), set up a plurality of equidistance outer sample mouthful (61) along its axial on outer sample tube (6).
2. The beauveria bassiana liquid fermentation tank of claim 1, wherein: the inner sampling pipe (5) is provided with scales corresponding to the outer sampling ports (61).
3. The beauveria bassiana liquid fermentation tank of claim 1, wherein: and rubber rings (62) are arranged on two sides of the outer sampling port (61).
4. The beauveria bassiana liquid fermentation tank of claim 1, wherein: the metal sheet (23) is a nickel-chromium electrothermal alloy sheet.
5. The beauveria bassiana liquid fermentation tank of claim 1, wherein: the tank body (1) is provided with a plurality of temperature sensors (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120028396.6U CN214270883U (en) | 2021-01-06 | 2021-01-06 | Beauveria bassiana liquid fermentation tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120028396.6U CN214270883U (en) | 2021-01-06 | 2021-01-06 | Beauveria bassiana liquid fermentation tank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214270883U true CN214270883U (en) | 2021-09-24 |
Family
ID=77790904
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120028396.6U Expired - Fee Related CN214270883U (en) | 2021-01-06 | 2021-01-06 | Beauveria bassiana liquid fermentation tank |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214270883U (en) |
-
2021
- 2021-01-06 CN CN202120028396.6U patent/CN214270883U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210924 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |