CN208395197U - A kind of microcarrier biological reactor - Google Patents
A kind of microcarrier biological reactor Download PDFInfo
- Publication number
- CN208395197U CN208395197U CN201820580235.6U CN201820580235U CN208395197U CN 208395197 U CN208395197 U CN 208395197U CN 201820580235 U CN201820580235 U CN 201820580235U CN 208395197 U CN208395197 U CN 208395197U
- Authority
- CN
- China
- Prior art keywords
- tank body
- valve body
- tank
- microcarrier
- interface
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn - After Issue
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of microcarrier biological reactor, and tank wall is equipped with interlayer and the jacket water (J.W.) of installation heating tube heating interlayer carrys out liquid in heating tank body in interlayer；Telescoping tube, can be easy to operate according to the big minor adjustment sample position of culture carrier amount for sampling in tank body；It installs the observation with transparent observing area additional and is connect in tank body, cooperation is corresponding to cross air filtration head and switching valve body, can flow into transparent pipe carrier cell, set and observe cell state under microscope at any time, and not waste remaining return in tank after observing；One carrier filter screen is installed in the bottom sides of reactor tank, cooperates snorkel, in harvest when final antigen removal carrier, avoids carrier plug filter net.The utility model improves microcarrier biological reactor culture apparatus, facilitates tank inner cell to observe in real time, facilitates removal waste fluid, facilitate filtration supports, improves efficiency, and improves adherence rate, improves cell density for 24 hours, the better cell of cultivation conditions.
Description
Technical field
The utility model relates to field of biotechnology more particularly to a kind of microcarrier biological reactors.
Background technique
Traditional microcarrier biological reactor is the mode of electric blanket heating to the heating method of pot liquid, passes through heating
Electric blanket, electric blanket are attached to outside reaction tank skin, are heated to pot liquid by heat transfer, the electric blanket temperature when just beginning to warm up
Very high, direct contact tank surface leads on the wall in tank that temperature is excessively high, will affect cell on the carrier in tank it is adherent with it is raw
It is long.
The length of probe tube is fixed in tank, when the culture of tank inner cell to for 24 hours after need to change liquid, the nutrition in drain tank
Then new nutrient solution is added in liquid, when arranging nutrient solution in tank, need first to adjust sampling length of tube before reactor tank assembles sterilizing,
The length of adjusting can only be estimated according to us using carrier amount, and in advance adjust inaccuracy, cannot thoroughly draining liq or
Carrier is discharged, Component Vectors are lost.
It needs to take out carrier cell outside tank when observing the cell in tank on carrier and goes to observe, the relatively more tired lock of operation, shadow
The virtual condition for ringing observation cell needs all culture solutions to include that carrier discharges outside the tank, then again when harvesting final antigen
The antigen harvested by one of filter screen filtration is with the mixed liquor of carrier, and time-consuming and laborious, carrier cell takes out and goes to observe outside tank, and one
Aspect wastes carrier cell, and another aspect cell can generate some variations after taking out, and the cell state observed has deviation.
Summary of the invention
The purpose of the utility model is to overcome the deficiencies in the prior art, provide a kind of microcarrier biological reactor, improve
Microcarrier biological reactor culture apparatus, facilitates tank inner cell to observe in real time, facilitates removal waste fluid, facilitates filtration supports, improves effect
Rate improves adherence rate, improves cell density for 24 hours, the better cell of cultivation conditions.
The technical solution adopted in the utility model is:
A kind of microcarrier biological reactor comprising tank body, tank body is interior to be equipped with blender, biological respinse detection electrode, disappears
Bulb apparatus, the upper surface of tank body are correspondingly provided with blender installing port, electrode interface and defoaming device interface, and blender is used for tank body
Interior liquid is stirred, and the bottom of tank body is equipped with outlet, and outlet extension has discharge pipe, and discharge pipe is equipped with discharge valve,
The inner wall of tank body, which corresponds to, is equipped with filter screen at outlet,
Tank body upper surface is additionally provided with intake interface and liquid material interface, and intake interface is used to be passed through gas to tank body different location
Body, liquid material interface include fluid infusion, sample tap, more than two feed inlets, and fluid infusion is used to supplement culture solution in incubation, into
Material mouth is used for the addition culture material into tank body,
Adjusting rod is installed, one end of adjusting rod is protruded into tank body, and the other end of adjusting rod is arranged with limit in sample tap
The other end of adjusting rod is limited in sample tap, and by adjusting the position change adjusting rod of limit sleeve and protrudes into tank by set, limit sleeve
The length of body is arranged with telescoping tube on one end of adjusting rod, and one end of telescoping tube is fixed on one end end of adjusting rod, telescoping tube
The other end connect probe tube,
Probe tube is equipped with the first mistake air filtration head and first switch valve body, and it is right that first switch valve body is located at the first air filtration excessively
One end of sample tap is answered, the other end of probe tube is separately connected one end of the second branch pipe and observation tube, and the second branch pipe other end is set
There is receiving flask, receiving flask is equipped with the 4th and crosses air filtration head, and the second branch pipe is equipped with the 4th switching valve body；Observation tube is set in level
It sets, observation tube has a transparent observing area, and the other end of observation tube is connected with second by third switching valve body and crosses air filtration head；Tank
The side wall of body has interlayer space, and jacket water (J.W.) is filled in interlayer space, and interlayer space is equipped with heating tube and heats to jacket water (J.W.), tank body
The lateral surface of side wall be equipped at intervals with the through-holes of two connection interlayer spaces, two through-holes are respectively connected to the both ends of a circulating pump.
Further, biological respinse detection electrode includes pH electrode, temperature electrode and dissolved oxygen electrode, and electrode interface includes pH
Electrode interface, temperature electrode interface and dissolved oxygen electrode interface.
Further, intake interface includes respirator interface, deep layer intake interface and surface layer intake interface.
Further, intake interface further includes strainer intake interface, and a snorkel is passed through tank body by strainer intake interface
Interior and opposite filter screen outlet.
Further, blender includes agitating shaft, and multiple agitating paddles are distributed on agitating shaft, and blender installing port is set to tank
At the center of the upper surface of body, agitating shaft installing port is fixed in one end of agitating shaft, and agitating shaft and agitating paddle constitute blender, stir
Mixing device is in be vertically arranged in tank body.
Further, the other end of observation tube is also connected with one end of the first branch pipe, and the first branch pipe is equipped with second switch valve
Body, the other end of the first branch pipe are equipped with the spare bottle that keeps sample, and the spare bottle that keeps sample crosses air filtration head equipped with third.Receiving flask
Further, probe tube, the first branch pipe and the second branch pipe are hose, first switch valve body, second switch valve body,
Third switching valve body and the 4th switching valve body are gas folder.
Further, probe tube, the first branch pipe and the second branch pipe are hard pipeline, first switch valve body, second switch
Valve body, third switching valve body and the 4th switching valve body are air valve.
The utility model uses above technical scheme, and it is two that the heating method of biological respinse pot liquid, which is designed as reactor tank,
Layer, centre have collet to install heating tube, and the water of collet is constantly recycled by peristaltic pump, the liquid in the water heating tank of collet.
Pipe is raised to position appropriate in removal waste fluid when culture carrier amount is big using telescopic pipe in tank inner part by the pipe of sampling
It sets, when culture carrier amount is small, pipe is dropped into position appropriate in removal waste fluid, carrier is unlikely to be discharged in this way.Outside tank
Partial probe tube connects a device, this device is placed on microscope and observes by the stopping when carrier cell flows through device
Cell state.Remaining return in tank can not be wasted after observing simultaneously.One carrier is installed in the bottom sides of reactor tank
Filter screen is equipped with a snorkel above filter screen, in harvest when final antigen removal carrier, opens above filter screen
Gas is unlikely to carrier plug filter net in filtering, filters unobstructed.The utility model has the advantages that sampling in 1, tank
Mouth is designed to what liftable was adjusted, can adjust the height of sample tap according to the height of liquid level of carrier amount in removal waste fluid, facilitate behaviour
Making simultaneously can be more thorough with drain.2, heating pot liquid uses heating tube heating tank internal jacket water, is flowed and is pressed from both sides by pump circulation
Water is covered, jacket water (J.W.) heating water bath pot liquid, such pot liquid temperature is uniform, and will not be locally too high.3, online observation is thin
Born of the same parents are connect by being installed additional in a pipeline and tank outside tank, are installed the transparent glass tube of a customization additional in tank Outer Tube, can be passed through
Suction flows into the carrier cell in tank in transparent pipe, sets and observes cell state under microscope at any time, while can incite somebody to action after observing
Remaining return in tank does not waste.4, a filtering mouth is filled in tank bottom side, filtering mouth is equipped with carrier filter screen, in harvest antigen
Carrier can be removed by filter screen filtration when liquid, carrier is easy filter screen to block during the filtration process, we are on filter screen
Side installs an air inlet pipe additional, opens ventilation during the filtration process, can drain the carrier on filter screen and be unlikely to plug filter net,
Much smoother harvest antigen.
Filtering again after previous harvest carrier and antigen needs 2-3 people to operate, using online when harvesting antigen after the utility model
Filtration supports only need 1 people to operate, and improve efficiency, and save labour.The utility model improves microcarrier biological reactor culture dress
It sets, facilitates tank inner cell to observe in real time, facilitate removal waste fluid, facilitate filtration supports, improve efficiency, improve adherence rate, improve
Cell density for 24 hours, the better cell of cultivation conditions.
Detailed description of the invention
The utility model is described in further details below in conjunction with the drawings and specific embodiments；
Fig. 1 is a kind of structural schematic diagram of microcarrier biological reactor of the utility model；
Fig. 2 is a kind of schematic cross-sectional view of microcarrier biological reactor of the utility model.
Specific embodiment
As shown in the figures 1 and 2, the utility model discloses a kind of microcarrier biological reactor comprising tank body 1 is set in tank body 1
There are blender, biological respinse detection electrode, defoaming device, the upper surface of tank body 1 is correspondingly provided with blender installing port 11, electrode connects
Mouth and defoaming device interface 12, blender are stirred for liquid in tank body 1, and the bottom of tank body 1 is equipped with outlet 13, discharge
13 extensions of mouth have discharge pipe 2, and discharge pipe 2 is equipped with discharge valve 21, and the inner wall of tank body 1, which corresponds to, is equipped with filter screen at outlet 13
Net 22,
1 upper surface of tank body is additionally provided with intake interface and liquid material interface, and intake interface is used to be passed through gas to 1 different location of tank body
Body, liquid material interface include fluid infusion 14, sample tap 15, more than two feed inlets 10, and fluid infusion 14 in incubation for supplementing
Culture solution, feed inlet 10 are used for the addition culture material into tank body 1,
Adjusting rod 3 is installed, one end of adjusting rod 3 is protruded into tank body 1, and the other end of adjusting rod 3 is arranged in sample tap 15
There is limit sleeve 31, limit sleeve 31 limits the other end of adjusting rod 3 in sample tap 15, and the position by adjusting limit sleeve 31 changes
Modified tone pole 3 protrudes into the length of tank body 1, is arranged with telescoping tube 4 on one end of adjusting rod 3, one end of telescoping tube 4 is fixed on adjusting
The other end of one end end of bar 3, telescoping tube 4 connects probe tube 5,
Probe tube 5 is equipped with first and crosses air filtration head F1 and first switch valve body K1, and first switch valve body K1 is located at the first mistake
One end of air filtration head F1 corresponding sample mouth 15, the other end of probe tube 5 are separately connected one end of the second branch pipe 30 and observation tube 6,
Second branch pipe, 30 other end is equipped with receiving flask 40, and receiving flask 40 is equipped with the 4th and crosses air filtration head F4, and the second branch pipe 30 is equipped with the
Four switching valve body K4；For observation tube 6 in being horizontally disposed with, observation tube 6 has a transparent observing area 61, and the other end of observation tube 6 passes through
Third switching valve body K3 is connected with second and crosses air filtration head F2；
The side wall of tank body 1 has interlayer space, and jacket water (J.W.) is filled in interlayer space, and interlayer space is equipped with 60 pairs of heating tube folders
Water heating is covered, the lateral surface of the side wall of tank body 1 is equipped at intervals with the through-hole 22 of two connection interlayer spaces, and two through-holes 22 connect respectively
Enter the both ends of a circulating pump 23.
Further, biological respinse detection electrode includes pH electrode, temperature electrode and dissolved oxygen electrode, and electrode interface includes pH
Electrode interface 17, temperature electrode interface 18 and dissolved oxygen electrode interface 19.
Further, intake interface includes respirator interface 24, deep layer intake interface 25 and surface layer intake interface 26.
Further, intake interface further includes strainer intake interface 27, and a snorkel 50 is logical by strainer intake interface 27
Enter in tank body 1 and against 22 outlet of filter screen.
Further, blender includes agitating shaft 28, and multiple agitating paddles 29, blender installing port are distributed on agitating shaft 28
11 are set at the center of the upper surface of tank body 1, and 28 installing port of agitating shaft, agitating shaft 28 and stirring are fixed in one end of agitating shaft 28
Paddle 29 constitutes blender, and blender is in be vertically arranged in tank body 1.
Further, the other end of receiving flask observation tube 6 is also connected with one end of the first branch pipe 7, and the first branch pipe is equipped with the
Two switching valve body K2, the other end of the first branch pipe 7 are equipped with the spare bottle 9 that keeps sample, and the spare bottle 9 that keeps sample crosses air filtration head equipped with third
F3.When the spare bottle 9 that keeps sample being set needing small-lot testing, the spare bottle 9 that keeps sample is used to collect sample.
Further, probe tube 5, the first branch pipe 7 and the second branch pipe 30 are hose, and first switch valve body K1, second open
Closing valve body K2, third switching valve body K3 and the 4th switching valve body K4 is gas folder.
Further, probe tube 5, the first branch pipe 7 and the second branch pipe 30 are hard pipeline, first switch valve body K1,
Two switching valve body K2, third switching valve body K3 and the 4th switching valve body K4 are air valve.
Further, the utility model further discloses a kind of side of application microcarrier biological reactor culture cell
Method comprising following steps:
Step 1, biological reactor opens preheating: the pH of biological reactor, temperature, dissolved oxygen electrode being connected, added anti-
The water for answering tank collet connects heating tube 60 and opens power supply heating, and opening circulating pump 23 water in tank is allowed to carry out circulation makes temperature
Degree is uniform.
Step 2, rolling bottle cell dissociation: the selection good rolling bottle PK-15 cell of cell state is disappeared using 0.25% pancreatin
Change liquid vitellophag, postdigestive cell sufficiently shakes up.
Step 3, cell count: taking appropriate postdigestive cell to be diluted with maintaining liquid, after appropriate dilution is taken after dilution
Cell adds to cell counting board and carries out cell count, calculates the cell density of cell liquid.
Step 4, tank culture on cell: addition microcarrier is risen by 3-5g/ into biological reactor, by 30-50 cell/micro-
Carrier adds cell；
Using thin needed for being calculated according to cell density after the quantity of microcarrier, every glomus cell number and rolling bottle digestion in reactor tank
Required cell concentration is added in biological reactor born of the same parents' amount by cell bottle pipeline corresponding with feed inlet；Adjust biological reactor
The cell culture parameter of related device: temperature: 36.5-37.5 DEG C, DO:50%-60%, pH:7.2-7.4, revolution: 40-60r/
Min is selected aerating system mode (Air/O2/CO2), is opened aerating system and is ventilated by intake interface to biological reactor
Culture.
Step 5, change liquid for 24 hours: when in biological reactor cell culture to for 24 hours after, stop stirring, stand 3-5 minute, adjusting
4 lower port of telescoping tube sampling height makes 4 lower port of telescoping tube sampling height at carrier ullage 0.5cm, discharge cell training
Then nutrient solution continues to cultivate by the cell maintenance medium that equivalent is added in fluid infusion 14.
The specific method of cell culture fluid is discharged in step 5: opening first switch valve body K1 and the 4th switching valve body K4, closes
Second switch valve body K2 and third switching valve body K3 are closed, aerating system is ventilated to tank body by intake interface and pressurizeed, and waste liquid is used
Bottle is used as receiving flask, and since pressure is greater than the pressure outside tank body in tank body, the culture solution of carrier ullage 0.5cm or more is being pressed
Probe tube is flowed through by telescoping tube under the action of power and constantly flows into receiving flask, until the liquid level of culture solution is lower than 4 lower end of telescoping tube
Stop when mouth sampling height；It is crossing air filtration head F1 respectively while closing first switch valve body K1 at this time and is making crossing air filtration head F2
It is inflated with syringe, the cell culture fluid in transparent observing area 61 and associated conduit part is squeezed into receiving flask.
Step 6, first switch valve body K1 and the cell observation: is opened after cell culture is to 48h, while being kept stirring
Three switching valve body K3 close second switch valve body K2 and the 4th switching valve body K4, use air-breathing in syringe crossing air filtration head F2,
The carrier and cell of biological respinse tank 1 stop pumping when flowing through transparent observing area 61, close first switch valve body K1, will
Microscopically observation is placed in cell observation area, after observation, first switch valve body K1 is opened, using syringe in crossing air filtration head F2
It inflates, the carrier and cell of transparent observing area 61 and associated conduit part is squeezed into tank body by the sampled mouth in former road.
Step 7, harvest antigen for the first time: after cell culture is to 72h, stop stirring, stand 3-5 minute, adjusting to take makes
Above 4 lower port of telescoping tube sampling height to carrier in tank at 0.5cm, collects first time antigen and harvest antigen liquid is set -15
Then refrigerator-freezer freezen protective below spending continues to cultivate by the cell maintenance medium that equivalent is added in fluid infusion 14.
It collects the specific method of first time antigen: opening first switch valve body K1 and the 4th switching valve body K4, close second
Switching valve body K2 and third switching valve body K3, aerating system by intake interface to tank body ventilate pressurize, use antigen bottle as
Receiving flask, since pressure is greater than the pressure outside tank body, the work of the culture solution of carrier ullage 0.5cm or more in pressure in tank body
It probe tube is flowed through by telescoping tube is constantly flowed into receiving flask with lower, until the liquid level of culture solution is sampled lower than 4 lower port of telescoping tube
Stop when height；It is crossing air filtration head F1 respectively while closing first switch valve body K1 at this time and is using injection crossing air filtration head F2
Device is inflated, and the cell culture fluid in transparent observing area 61 and associated conduit part is squeezed into receiving flask.
Step 8, it after-crop antigen and changes liquid culture: after biological reactor cell culture is to 96h, stopping stirring,
3-5 minutes are stood, adjusting makes above 4 lower port of telescoping tube sampling height to carrier in tank at 0.5cm, again by sample tap 15
Culture supernatant antigen liquid is harvested, the second antigen liquid of harvest is set into the lower refrigerator-freezer freezen protective of -15 degree.
It collects the specific method of second of antigen: opening first switch valve body K1 and the 4th switching valve body K4, close second
Switching valve body K2 and third switching valve body K3, aerating system by intake interface to tank body ventilate pressurize, use antigen bottle as
Receiving flask, since pressure is greater than the pressure outside tank body, the work of the culture solution of carrier ullage 0.5cm or more in pressure in tank body
It probe tube is flowed through by telescoping tube is constantly flowed into receiving flask with lower, until the liquid level of culture solution is sampled lower than 4 lower port of telescoping tube
Stop when height；It is crossing air filtration head F1 respectively while closing first switch valve body K1 at this time and is using injection crossing air filtration head F2
Device is inflated, and the cell culture fluid in transparent observing area 61 and associated conduit part is squeezed into receiving flask.
Step 9, third time harvest antigen: after the cell culture in biological reactor is to 96-120h, adjusting revolution is
100-150 turns 3-5 minutes, then stops stirring, stands 3-5 minutes, is added by aerating system from intake interface to tank body ventilation
Pressure, open discharge pipe 2 on discharge valve 21 harvest filtered antigen, while open the snorkel 50 above filter screen into
Gas guarantees to harvest filtered antigen and keep sample to detect antigen valence, smoothly harvests antigen, the third that will finally harvest
Secondary antigen liquid sets -15 degree or less refrigerator-freezer freezen protective.
The utility model uses above technical scheme, and it is two that the heating method of biological respinse pot liquid, which is designed as reactor tank,
Layer, centre have collet to install heating tube 60, and the water of collet is constantly recycled by peristaltic pump, the liquid in the water heating tank of collet
Body.The pipe of sampling uses telescopic pipe in tank inner part, when culture carrier amount is big, is raised to pipe in removal waste fluid appropriate
Pipe is dropped to position appropriate in removal waste fluid when culture carrier amount is big by position, and carrier is unlikely to be discharged in this way.In tank
The probe tube 5 of outer portion connects a device, this device is placed on microscope and sees by the stopping when carrier cell flows through device
Examine cell state.Remaining return in tank can not be wasted after observing simultaneously.One load is installed in the bottom sides of reactor tank
Body filter screen is equipped with a snorkel 50 above filter screen, in harvest when final antigen removal carrier, opens on filter screen
The gas of side is unlikely to carrier plug filter net in filtering, filters unobstructed.The utility model has the advantages that in 1, tank
Sample tap 15 is designed to what liftable was adjusted, can adjust the height of sample tap 15 according to the height of liquid level of carrier amount in removal waste fluid
Degree, facilitate operation and meanwhile can be more thorough with drain.2, heating pot liquid uses 60 heating tank internal jacket water of heating tube, passes through pump
Jacket water (J.W.) is circulated, jacket water (J.W.) heating water bath pot liquid, such pot liquid temperature is uniform, and will not be locally too high.3,
Online observation cell is connect by being installed additional in a pipeline and tank outside tank, installs the transparent glass of a customization additional in tank Outer Tube
Carrier cell in tank can be flowed into transparent pipe by suction, set and observe cell state under microscope at any time, see simultaneously by pipe
Remaining return in tank can not be wasted after examining.4, a filtering mouth being filled in tank bottom side, filtering mouth is equipped with carrier filter screen,
Carrier can be removed by filter screen filtration when harvesting antigen liquid, carrier is easy filter screen to block during the filtration process, we
It installs an air inlet pipe additional above filter screen, opens ventilation during the filtration process, the carrier for filtering mouth can be drained and be unlikely to stifled
Firmly filter mouth, much smoother harvest antigen.Filtering again after previous harvest carrier and antigen needs 2-3 people to operate, practical new using this
On-line filtration carrier only needs 1 people to operate when harvesting antigen after type, improves efficiency, and saves labour.The utility model improves micro- load
Body biological reactor culture apparatus, facilitates tank inner cell to observe in real time, facilitates removal waste fluid, facilitate filtration supports, improve efficiency, mention
High adherence rate improves cell density for 24 hours, the better cell of cultivation conditions.
Claims (8)
1. a kind of microcarrier biological reactor, it is characterised in that: it includes tank body, blender is equipped in tank body, biological respinse is visited
Electrode, defoaming device are surveyed, the upper surface of tank body is correspondingly provided with blender installing port, electrode interface and defoaming device interface, stirs
Device is stirred for liquid in tank body, and the bottom of tank body is equipped with outlet, and outlet extension has discharge pipe, and discharge pipe is equipped with
Discharge valve, the inner wall of tank body, which corresponds to, is equipped with filter screen at outlet,
Tank body upper surface is additionally provided with intake interface and liquid material interface, and intake interface is used to be passed through gas, liquid to tank body different location
Material interface includes fluid infusion, sample tap, more than two feed inlets, and fluid infusion is for supplementing culture solution, feed inlet in incubation
For adding culture material into tank body,
Adjusting rod is installed, one end of adjusting rod is protruded into tank body, and the other end of adjusting rod is arranged with limit sleeve, limit in sample tap
Position set limits the other end of adjusting rod in sample tap, and the length of tank body is protruded by adjusting the position change adjusting rod of limit sleeve
Degree, is arranged with telescoping tube on one end of adjusting rod, and one end of telescoping tube is fixed on one end end of adjusting rod, telescoping tube it is another
End connection probe tube,
Probe tube is equipped with the first air filtration head and first switch valve body, first switch valve body excessively and is located at the first air filtration head correspondence excessively and takes
One end of sample mouth, the other end of probe tube are separately connected one end of the second branch pipe and observation tube, and the second branch pipe other end, which is equipped with, to be received
Collect bottle, receiving flask is equipped with the 4th and crosses air filtration head, and the second branch pipe is equipped with the 4th switching valve body；Observation tube is in horizontally disposed, sight
Pipe is examined with a transparent observing area, the other end of observation tube is connected with second by third switching valve body and crosses air filtration head；
The side wall of tank body has interlayer space, and jacket water (J.W.) is filled in interlayer space, and interlayer space is equipped with heating tube and adds to jacket water (J.W.)
Heat, the lateral surface of the side wall of tank body are equipped at intervals with the through-hole of two connection interlayer spaces, and two through-holes are respectively connected to a circulating pump
Both ends.
2. a kind of microcarrier biological reactor according to claim 1, it is characterised in that: biological respinse detection electrode includes
PH electrode, temperature electrode and dissolved oxygen electrode, electrode interface include pH electrode interface, temperature electrode interface and dissolved oxygen electrode interface.
3. a kind of microcarrier biological reactor according to claim 1, it is characterised in that: intake interface includes that respirator connects
Mouth, deep layer intake interface and surface layer intake interface.
4. a kind of microcarrier biological reactor according to claim 3, it is characterised in that: intake interface further includes strainer
Intake interface, a snorkel is passed through in tank body by strainer intake interface and opposite filter screen outlet.
5. a kind of microcarrier biological reactor according to claim 1, it is characterised in that: blender includes agitating shaft, is stirred
It mixes and multiple agitating paddles is distributed on axis, blender installing port is set at the center of the upper surface of tank body, and one end of agitating shaft is fixed
In agitating shaft installing port, agitating shaft and agitating paddle constitute blender, and blender is in be vertically arranged in tank body.
6. a kind of microcarrier biological reactor according to claim 1, it is characterised in that: the other end of observation tube also connects
One end of the first branch pipe is connect, the first branch pipe is equipped with second switch valve body, and the other end of the first branch pipe is equipped with the spare bottle that keeps sample, standby
Air filtration head is crossed equipped with third with bottle is kept sample.
7. a kind of microcarrier biological reactor according to claim 6, it is characterised in that: probe tube, the first branch pipe and
Two branch pipes are hose, and first switch valve body, second switch valve body, third switching valve body and the 4th switching valve body are gas folder.
8. a kind of microcarrier biological reactor according to claim 6, it is characterised in that: probe tube, the first branch pipe and
Two branch pipes are hard pipeline, and first switch valve body, second switch valve body, third switching valve body and the 4th switching valve body are gas
Valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820580235.6U CN208395197U (en) | 2018-04-23 | 2018-04-23 | A kind of microcarrier biological reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820580235.6U CN208395197U (en) | 2018-04-23 | 2018-04-23 | A kind of microcarrier biological reactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208395197U true CN208395197U (en) | 2019-01-18 |
Family
ID=65061330
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820580235.6U Withdrawn - After Issue CN208395197U (en) | 2018-04-23 | 2018-04-23 | A kind of microcarrier biological reactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208395197U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108441424A (en) * | 2018-04-23 | 2018-08-24 | 福州大北农生物技术有限公司 | A kind of microcarrier biological reactor and its culture porcine circovirus 2 type method |
-
2018
- 2018-04-23 CN CN201820580235.6U patent/CN208395197U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108441424A (en) * | 2018-04-23 | 2018-08-24 | 福州大北农生物技术有限公司 | A kind of microcarrier biological reactor and its culture porcine circovirus 2 type method |
| CN108441424B (en) * | 2018-04-23 | 2023-08-22 | 福州大北农生物技术有限公司 | Microcarrier biological reaction tank and method for culturing porcine circovirus type 2 by same |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108441424A (en) | A kind of microcarrier biological reactor and its culture porcine circovirus 2 type method | |
| CN106754366A (en) | For the disposable intelligent digester systems of animal cell culture | |
| CN101899394A (en) | External circulation animal cell culture bioreactor | |
| CN208395197U (en) | A kind of microcarrier biological reactor | |
| CN108707521A (en) | A kind of universal wine fermentation tank | |
| CN206814791U (en) | Disposable intelligent digester systems for animal cell culture | |
| CN215161393U (en) | Constant-temperature anaerobic reaction device | |
| CN208684889U (en) | Wine fermentation tank | |
| CN201261780Y (en) | Pot for adding diatomite for filtering beer | |
| CN108040957A (en) | Dissolved oxygen tank and breeding water body aeration system | |
| CN207891348U (en) | A kind of crop leaf endophyte air stirring installation for fermenting | |
| CN209185419U (en) | A kind of constant temperature aquatic product cultivating pool | |
| CN207031442U (en) | Aerobic fermentation system | |
| CN208667686U (en) | A kind of fermented tea installation for fermenting | |
| CN206266628U (en) | Culture device for liquid strain of edible fungus | |
| CN208949309U (en) | A kind of automatic cytological culture apparatus for simulating organismic internal environment | |
| CN208071693U (en) | Small aerobic fermentation tank capable of integrally sampling | |
| CN214571994U (en) | Small-size multi-functional cell culture jar | |
| CN108624434A (en) | Wine fermentation tank | |
| CN109370883A (en) | A kind of multi-direction stirring biological fermentation tank of temperature with high efficiency | |
| CN216499132U (en) | Automatic blending device of nutrient solution | |
| CN205635580U (en) | Biogas digester | |
| CN216864188U (en) | Constant-temperature circulating fermentation system for preparing fermented fish | |
| CN219168340U (en) | Ozone adding equipment | |
| CN221490953U (en) | Medical gynecological flushing device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: Gushan Town Park in Jinan District of Fuzhou city in Fujian province village 110 350000 Patentee after: Zhaofenghua Biotechnology (Fuzhou) Co.,Ltd. Address before: Gushan Town Park in Jinan District of Fuzhou city in Fujian province village 110 350000 Patentee before: FUZHOU DA BEI NONG BIOTECH Co.,Ltd. |
|
| CP01 | Change in the name or title of a patent holder | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20190118 Effective date of abandoning: 20230822 |
|
| AV01 | Patent right actively abandoned |
Granted publication date: 20190118 Effective date of abandoning: 20230822 |
|
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |