CN214422648U - PCR instrument and temperature control system of PCR instrument - Google Patents
PCR instrument and temperature control system of PCR instrument Download PDFInfo
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- CN214422648U CN214422648U CN202023279908.0U CN202023279908U CN214422648U CN 214422648 U CN214422648 U CN 214422648U CN 202023279908 U CN202023279908 U CN 202023279908U CN 214422648 U CN214422648 U CN 214422648U
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Abstract
The utility model discloses a temperature control system of PCR appearance, this temperature control system of PCR appearance include test tube seat and control module, a plurality of test tube chambeies have on the test tube seat, the test tube seat has a plurality of first constant temperature fluid jars through the tube coupling, first constant temperature fluid jar be used for to the test tube seat carries the fluid of different temperatures, control module with first constant temperature fluid jar electricity is connected. The utility model discloses be favorable to improving PCR thermal cycle alternating temperature speed, realize the target of quick PCR experiment. Furthermore, the utility model discloses a PCR appearance.
Description
Technical Field
The utility model relates to a PCR appearance technical field, concretely relates to temperature control system of PCR appearance and PCR appearance.
Background
The PCR reaction is divided into three stages of denaturation, annealing and extension, each stage corresponding to a temperature of about: and (3) denaturation temperature: 94 ℃ C. +/-3C, annealing temperature: 55 ℃ C. +/-5℃ and extension temperature: 72 ℃ and +/-5 ℃, and the constant temperature range is determined according to the active temperature of the enzyme adopted by different reagents.
When the temperature change control is performed by the conventional PCR instrument, the temperature change control is generally performed by a semiconductor refrigeration module. The disadvantages are that the thermal conversion efficiency is low, the temperature change speed is low, the precision is difficult to control, and the PCR reaction time is overlong.
SUMMERY OF THE UTILITY MODEL
The utility model discloses a main aim at provides a temperature control system of PCR appearance and PCR appearance to solve the technical problem who proposes in the background art.
In order to achieve the above object, the utility model provides a temperature control system of PCR appearance, temperature control system of this PCR appearance include test tube seat and control module, a plurality of test tube chambeies have on the test tube seat, the test tube seat has a plurality of first constant temperature fluid tanks through the tube coupling, first constant temperature fluid tank be used for to the fluid of different temperatures is carried to the test tube seat, control module with first constant temperature fluid tank electricity is connected.
Preferably, the test tube seat is internally provided with a first internal pipeline, and two ends of the first internal pipeline are exposed out of the side surface of the test tube seat; the first constant-temperature fluid tank comprises a first tank body, a first temperature control component and a first driving pump, wherein the first temperature control component and the first driving pump are arranged in the first tank body, the first tank body is connected with the output end of the first internal pipeline through a pipeline, and the output end of the first driving pump is connected with the input end of the first internal pipeline through a pipeline.
Preferably, the first constant temperature fluid tank is three.
Preferably, still be provided with supplementary temperature control assembly on the test tube seat, supplementary temperature control assembly is arranged including the laminating temperature adjusting sheet on the test tube seat, control module is connected with supplementary temperature control assembly electricity.
Preferably, the temperature control piece is an electrically-driven temperature changing device, the temperature control system of the PCR instrument further comprises a base, the top surface of the base is provided with a containing cavity, the electrically-driven temperature changing device and at least the bottom of the test tube seat are located in the containing cavity, and the auxiliary temperature control assembly further comprises a fluid circulating device connected with the base through a pipeline.
Preferably, a second internal pipeline is arranged inside the base, and two ends of the second internal pipeline are exposed out of the side face of the base; fluid circulating device includes second constant temperature fluid jar and cooling unit, the second constant temperature fluid jar includes the second jar of body and sets up internal second driving pump and the second temperature control part of second jar, the output of second driving pump pass through the pipeline with the input of second internal pipeline is connected, the input of the second jar of body passes through cooling unit with the output of second internal pipeline is connected.
Preferably, the cooling unit includes a heat dissipation block, a third internal pipeline is arranged in the heat dissipation block, two ends of the third internal pipeline are exposed out of the side face of the heat dissipation block, two ends of the second internal pipeline are respectively connected with the output end of the second internal pipeline and the second tank body through pipelines, and a plurality of heat dissipation fins are arranged on the surface of the heat dissipation block.
Preferably, the cooling unit further includes a fan disposed on the heat dissipation block.
Preferably, a temperature sensor electrically connected with the control module is embedded on the inner wall of the test tube cavity.
The utility model provides a PCR appearance, this PCR appearance include the temperature control system of above-mentioned PCR appearance, and the temperature control system of this PCR appearance includes test tube seat and control module, a plurality of test tube chambeies have on the test tube seat, the test tube seat has a plurality of first constant temperature fluid tanks through the tube coupling, first constant temperature fluid tank be used for to the test tube seat carries the fluid of different temperatures, control module with first constant temperature fluid tank electricity is connected.
The embodiment of the utility model provides a temperature control system of PCR appearance inputs the fluid of different temperatures at each stage of PCR reaction through a plurality of first constant temperature fluid tanks of control module control to be favorable to improving the efficiency that the temperature switches, in order to be favorable to reducing the time of PCR reaction.
Drawings
FIG. 1 is a schematic structural diagram of an embodiment of a temperature control system of a PCR instrument according to the present invention;
FIG. 2 is a schematic view showing a partial structure of a temperature control system of the PCR instrument shown in FIG. 1;
FIG. 3 is a schematic view of the first constant temperature fluid tank shown in FIG. 2;
fig. 4 is a schematic structural view of the second constant temperature fluid tank shown in fig. 2.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention, and all other embodiments obtained by those skilled in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
The utility model provides a temperature control system of PCR appearance, as shown in FIG. 1 and FIG. 2, temperature control system of this PCR appearance includes test tube seat 100 and control module 300, has a plurality of test tube chambeies on the test tube seat 100, and test tube seat 100 has a plurality of first constant temperature fluid jar 300 through the pipe connection, and first constant temperature fluid jar 300 is used for carrying the fluid of different temperatures to test tube seat 100, and control module 200 is connected with first constant temperature fluid jar 300 electricity.
In this embodiment, the test tube holders 100 may be arranged according to actual conditions, and the sizes and the number of the test tube cavities may also be arranged according to actual conditions. Meanwhile, the number of the first constant temperature fluid tanks 300 is plural, so as to respectively supply fluids (fluid refers to liquid and gas, such as mineral oil) with different temperatures to the test tube block 100, that is, the fluids in the plural first constant temperature fluid tanks 300 maintain different temperatures, so that the control module 200 respectively controls the corresponding first constant temperature fluid tanks 300 to supply the fluids with the temperatures required by each stage of the PCR reaction to the test tube block 100. If the existing PCR reaction is divided into three stages, which correspond to three temperatures, the fluids with one-to-one corresponding temperatures in the first constant temperature fluid tanks 300 can be utilized, so that the purpose of rapidly switching the temperature in the test tube holder 100 is achieved, the efficiency of temperature switching is increased, and the reduction of the PCR reaction time is facilitated.
In a preferred embodiment, as shown in fig. 2 and 3, it is preferable that the test tube holder 100 has a first internal pipeline therein, and two ends of the first internal pipeline are exposed out of the test tube holder 100, and the first internal pipeline is disposed inside the test tube holder 100 according to the arrangement pattern of the existing heat dissipation pipeline. Meanwhile, it is preferable that the first constant temperature fluid tank 300 includes a first tank body 310, a first driving pump 320, and a first temperature control part 330, the first tank body 310 is connected to an output end of the first internal pipe through a pipe, and an output end of the first driving pump 320 is connected to an input end of the first internal pipe through a pipe. At this time, it is preferable that the output end of the first driving pump 320 is provided with a solenoid valve (which is also electrically connected to the control module 200) so as to control the automatic opening and closing of the pipeline thereof. Wherein, first jar of body 310 adopts current metal can body can, and the surface cladding of preferred metal can body has the heat preservation to be favorable to avoiding thermal loss, first drive pump 320 adopts current fluid pump that can carry high temperature can, and first temperature control part 330 sets up in first jar of body 310, and has the function of heating and temperature detection, like the form of temperature detection part + heating part can. At this time, the first driving pump 320 and the first temperature control unit 330 are electrically connected to the control module 200, so that the control module 200 controls the first temperature control unit 330 to heat the fluid in the first tank 310 to a preset temperature and controls the start and stop of the first driving pump 320.
In a preferred embodiment, the first constant temperature fluid tanks 300 are three, that is, the three first constant temperature fluid tanks 300 are respectively filled with the fluids with the temperatures of 55 ℃, 72 ℃ and 94 ℃, so that the test tube holders 100 can rapidly reach the temperatures required by the PCR reaction at various stages. Specifically, one of the first thermostated fluid tanks 300 outputs fluid at about 55 ℃ (i.e. 55 ℃ +/-5 ℃), the other first thermostated fluid tank 300 outputs fluid at about 72 ℃ (i.e. 72 ℃ +/-5 ℃), and the last first thermostated fluid tank 300 outputs fluid at about 94 ℃ (i.e. 94 ℃ +/-3 ℃).
In a preferred embodiment, as shown in fig. 1 and fig. 2, an auxiliary temperature control assembly 400 is further disposed on the test tube socket 100, the auxiliary temperature control assembly 400 includes a temperature adjusting sheet 410 disposed on the test tube socket 100, and the control module 200 is electrically connected to the auxiliary temperature control assembly 400. The temperature control sheet 410 is preferably located at the bottom of the test tube holder 100, and the temperature control sheet 410 may be used for heating and/or cooling the test tube holder 100. As for the temperature adjusting sheet 410, the specific manner of adjusting the temperature of the base is as follows: when the temperature control sheet 410 can only perform heating or cooling, it is preferable that the temperature of the fluid delivered by the first constant temperature fluid tank 300 is slightly lower or higher than the preset temperature, so as to facilitate the temperature control of the test tube holder 100 by the temperature control sheet 410 within a precise range, and when the temperature control sheet 410 can perform heating and cooling simultaneously, it is preferable that the temperature of the fluid delivered by the first constant temperature fluid tank 300 is the preset temperature. In this embodiment, the first constant temperature fluid tank 300 is used to convey the fluid with the corresponding temperature to the test tube holder 100, and then the temperature adjusting sheet 410 is used to accurately adjust the temperature of the test tube holder 100, that is, the test tube holder 100 is heated or cooled according to the real-time temperature of the test tube holder 100, so as to ensure that the temperature of the test tube holder 100 is stabilized at the preset temperature, thereby being beneficial to improving the control precision of the heating temperature.
In a preferred embodiment, as shown in fig. 2, the temperature control plate 410 is preferably an electrically driven temperature varying device, the temperature control system of the PCR instrument further includes a base 110, the top surface of the base 110 has a receiving cavity, the electrically driven temperature varying device and at least the bottom of the test tube holder 100 are located in the receiving cavity, and the auxiliary temperature control assembly 400 further includes a fluid circulation device 420 connected to the base 110 through a pipeline. The electrically driven temperature varying device is a semiconductor chilling plate, so as to heat or dissipate heat from the test tube holder 100, and meanwhile, in order to increase the heat exchange performance of the semiconductor chilling plate, a fluid at a constant temperature (e.g., 40 ℃) is delivered to the base 110 through the fluid circulating device 420, and as for the base 110, a heat insulating material is required, so as to avoid excessive heat loss.
In a preferred embodiment, as shown in fig. 2 and 4, it is preferable that the base 110 has a second inner pipe therein, and two ends of the second inner pipe are exposed to the side of the base 110; the fluid circulation device 420 includes a second constant temperature fluid tank 421 and a cooling unit 422, the second constant temperature fluid tank 421 includes a second tank 423 and a second driving pump 424 and a second temperature control part 425 disposed in the second tank 423, an output end of the second driving pump 424 is connected with an input end of a second internal pipe through a pipe, and an input end of the second tank 421 is connected with an output end of the second internal pipe through the cooling unit 422. The second internal pipe may be arranged inside the base 110 in a manner of referring to an arrangement pattern of the existing heat dissipation pipe, and the second constant temperature fluid tank 421 may be arranged in a manner of referring to the first constant temperature fluid tank 300. At this time, the second driving pump 424, the second temperature control component 425 and the cooling unit 422 are electrically connected to the control module 200, so that when the test tube holder 100 needs to dissipate heat, the control module 200 controls the cooling unit 422 to start and controls the second temperature control component 425 to stop heating the fluid, so as to dissipate heat of the fluid by using the cooling unit 422 to reduce the temperature of the fluid (i.e., reduce the temperature of the fluid increased by the passing through the semiconductor chilling plate), and when the test tube holder 100 needs to be heated, the control module 200 controls the cooling unit 422 to close and controls the second temperature control component 425 to heat the fluid, so as to increase the temperature of the fluid (i.e., the temperature of the supplementary fluid lost by the passing through the semiconductor chilling plate).
In a preferred embodiment, the preferred cooling unit 422 includes a heat dissipation block, and the preferred heat dissipation block has a third internal pipeline therein, two ends of the third internal pipeline are exposed to the side of the heat dissipation block, and two ends of the third internal pipeline are respectively connected to the output end of the second internal pipeline and the second tank 423, and the third internal pipeline may be arranged inside the heat dissipation block according to the arrangement pattern of the existing heat dissipation pipeline. Meanwhile, the radiating block is preferably made of a metal material, such as copper, and the surface of the radiating block is provided with a plurality of radiating fins, so that the radiating efficiency is increased by increasing the radiating area.
In a preferred embodiment, the cooling unit 422 further includes a fan disposed on the heat sink, and the fan is further electrically connected to the control module 200, so as to facilitate active heat dissipation of the heat sink by the fan, thereby facilitating increase of heat dissipation efficiency.
In a preferred embodiment, a temperature sensor electrically connected to the control module 200 is embedded in an inner wall of the test tube cavity, and an accommodating groove capable of accommodating the temperature sensor is disposed in the inner wall of the test tube cavity, so that the control module 200 can control the operating state of the temperature control sheet 410 according to the temperature collected by the temperature sensor.
In a preferred embodiment, the temperature adjusting sheet 410 is preferably a heating sheet, and in this case, the test tube holder 100 is preferably provided with a heat insulation cover covering the heating sheet, and as for the heating sheet, the heating sheet can be selected from any one of the existing heating sheets with different specifications, such as a silica gel heating sheet, a mica heating sheet, and an MCH heating sheet.
The utility model discloses further provide the PCR appearance, this PCR appearance includes the temperature control system of the PCR appearance in above-mentioned embodiment, and the temperature control system's of this PCR appearance concrete structure refers to above-mentioned embodiment, because the temperature control system of this PCR appearance has adopted the whole technical scheme of above-mentioned all embodiments, consequently has all beneficial effects that the technical scheme of above-mentioned embodiment brought at least, and the repeated description is no longer given here.
The above is only the part or the preferred embodiment of the present invention, no matter the characters or the drawings can not limit the protection scope of the present invention, all under the whole concept of the present invention, the equivalent structure transformation performed by the contents of the specification and the drawings is utilized, or the direct/indirect application in other related technical fields is included in the protection scope of the present invention.
Claims (10)
1. The temperature control system of the PCR instrument is characterized by comprising a test tube seat and a control module, wherein the test tube seat is provided with a plurality of test tube cavities, the test tube seat is connected with a plurality of first constant-temperature fluid tanks through pipelines, the first constant-temperature fluid tanks are used for conveying fluids with different temperatures to the test tube seat, and the control module is electrically connected with the first constant-temperature fluid tanks.
2. The temperature control system of the PCR instrument as claimed in claim 1, wherein the test tube holder has a first internal tube therein, and both ends of the first internal tube are exposed to the side surfaces of the test tube holder; the first constant-temperature fluid tank comprises a first tank body, a first temperature control component and a first driving pump, wherein the first temperature control component and the first driving pump are arranged in the first tank body, the first tank body is connected with the output end of the first internal pipeline through a pipeline, and the output end of the first driving pump is connected with the input end of the first internal pipeline through a pipeline.
3. The temperature control system of the PCR machine according to claim 1 or 2, wherein the first constant temperature fluid tank is three.
4. The temperature control system of the PCR instrument as claimed in claim 1, wherein the test tube holder is further provided with an auxiliary temperature control assembly, the auxiliary temperature control assembly comprises a temperature adjusting sheet attached to the test tube holder, and the control module is electrically connected to the auxiliary temperature control assembly.
5. The temperature control system of the PCR instrument as claimed in claim 4, wherein the temperature control plate is an electrically driven temperature varying device, the temperature control system of the PCR instrument further comprises a base, the top surface of the base has a receiving cavity, the electrically driven temperature varying device and at least the bottom of the test tube holder are located in the receiving cavity, and the auxiliary temperature control assembly further comprises a fluid circulation device connected with the base through a pipeline.
6. The temperature control system of the PCR instrument as claimed in claim 5, wherein the base has a second inner tube, and two ends of the second inner tube are exposed to the side of the base; fluid circulating device includes second constant temperature fluid jar and cooling unit, the second constant temperature fluid jar includes the second jar of body and sets up internal second driving pump and the second temperature control part of second jar, the output of second driving pump pass through the pipeline with the input of second internal pipeline is connected, the input of the second jar of body passes through cooling unit with the output of second internal pipeline is connected.
7. The temperature control system of the PCR instrument as claimed in claim 6, wherein the cooling unit comprises a heat dissipation block, a third internal pipeline is arranged in the heat dissipation block, two ends of the third internal pipeline are exposed out of the side face of the heat dissipation block, two ends of the second internal pipeline are respectively connected with the output end of the second internal pipeline and the second tank body through pipelines, and a plurality of heat dissipation fins are arranged on the surface of the heat dissipation block.
8. The temperature control system of the PCR instrument of claim 7, wherein the cooling unit further comprises a fan disposed on the heat dissipation block.
9. The temperature control system of the PCR instrument as claimed in claim 1, wherein a temperature sensor electrically connected to the control module is embedded on the inner wall of the test tube chamber.
10. A PCR instrument comprising the temperature control system of the PCR instrument of any one of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023279908.0U CN214422648U (en) | 2020-12-29 | 2020-12-29 | PCR instrument and temperature control system of PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023279908.0U CN214422648U (en) | 2020-12-29 | 2020-12-29 | PCR instrument and temperature control system of PCR instrument |
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| Publication Number | Publication Date |
|---|---|
| CN214422648U true CN214422648U (en) | 2021-10-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202023279908.0U Active CN214422648U (en) | 2020-12-29 | 2020-12-29 | PCR instrument and temperature control system of PCR instrument |
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|---|---|
| CN (1) | CN214422648U (en) |
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- 2020-12-29 CN CN202023279908.0U patent/CN214422648U/en active Active
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220321 Address after: 519000 floor 2, phase II plant, No. 2, Chuangxin Fourth Road, high tech Zone, Zhuhai, Guangdong Patentee after: ZHUHAI BLACK HORSE BIOTECHNOLOGY Co.,Ltd. Patentee after: Zhuhai Heima Medical Instrument Co., Ltd Address before: 519000 floor 2, phase II plant, No. 2, Chuangxin Fourth Road, high tech Zone, Zhuhai, Guangdong Patentee before: ZHUHAI BLACK HORSE BIOTECHNOLOGY Co.,Ltd. |
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| TR01 | Transfer of patent right |