CN214325779U - ELISA kit for detecting tumor necrosis factor TNF-alpha - Google Patents
ELISA kit for detecting tumor necrosis factor TNF-alpha Download PDFInfo
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- CN214325779U CN214325779U CN202023069693.XU CN202023069693U CN214325779U CN 214325779 U CN214325779 U CN 214325779U CN 202023069693 U CN202023069693 U CN 202023069693U CN 214325779 U CN214325779 U CN 214325779U
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Abstract
The utility model provides an ELISA kit for detecting tumor necrosis factor TNF-alpha. Specifically, the kit comprises: a box body and a box cover; the object placing plate comprises a reagent area, an antibody area and an instrument area, and each area is provided with an object placing groove; the reagent area is provided with a standard sample tube, a treatment liquid tube, a cleaning liquid tube and a TMB display liquid tube; the antibody area is provided with a primary antibody tube, a secondary antibody tube and a stop liquid tube; the instrument area is provided with a sterile independent packaging culture pore plate, a photophobic independent packaging covering antigen linked pore plate, a ninety-six pore plate covering rubber plate film and a ninety-six pore plate frame which are arranged in parallel; a light-shielding bag is also arranged on one side of the instrument area; the light shielding bag is arranged in the instrument area, and the light shielding bag is arranged in the instrument area. The utility model discloses a kit convenient to use can accelerate the operating speed.
Description
Technical Field
The utility model relates to a reagent immunology field specifically is an ELISA kit that detects CAR-T cell TNF-alpha release.
Background
The ELISA test is an experimental diagnosis method with high sensitivity, strong specificity and good repeatability, and has been widely applied to various fields of immunology due to factors such as stable reagent, easy storage, simple and convenient operation, objective result judgment and the like.
One of the successful manifestations of CAR-T production is that it can cause relatively high level of cytokine release, TNF-alpha is one of these cytokines, its release can effectively evaluate the function of CAR-T, before evaluating the cytokine release level of CAR-T, it needs to first culture CAR-T cell and tumor cell together for a period of time, then detect factor release, there are many ELISA detection kits of TNF-alpha on the market today, the principle is basically the same, detect the content of TNF-alpha by antigen-antibody reaction in combination with TMB color development. However, the TNF-alpha of the kits has the defects of low standard substance dilution concentration, no cell co-culture material, inconvenient use and the like.
Therefore, there is a need in the art to provide a TNF-a detection kit for CAR-T cell release that is more convenient to handle.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a TNF-alpha detection kit to CAR-T cell release that the operation is more convenient.
The utility model provides a detect ELISA kit of tumor necrosis factor TNF-alpha, the kit includes:
the box body is provided with an opening at the upper part, and the box cover is used for sealing the opening;
the object placing plate comprises a reagent area, an antibody area and an instrument area, wherein each area is provided with a plurality of object placing grooves with upper openings, and the object placing grooves are used for placing reagent tubes or instruments;
wherein the reagent area is provided with a standard sample tube, a treatment liquid tube, a cleaning liquid tube and a TMB display liquid tube;
the antibody area is provided with a primary antibody tube, a secondary antibody tube and a termination liquid tube;
the instrument area is provided with a sterile independent packaging culture pore plate, a photophobic independent packaging covering antigen linked pore plate, a ninety-six pore plate covering rubber plate film and a ninety-six pore plate frame which are arranged in parallel;
the light-shading bag is arranged on one side of the instrument area of the object placing plate;
the light-proof bag is arranged in the instrument area, and the light-proof bag is arranged in the instrument area and is used for containing the bacteria-free independent packaging culture pore plate, the light-proof independent packaging covering antigen linked pore plate, the ninety-six pore plate covering rubber plate film and the ninety-six pore plate frame.
In another preferred embodiment, the shelf further has a removable complementary piece having a recess for receiving a portion of the instrument located outside the shelf for complementary mounting with the shelf on which the instrument is loaded.
In another preferred example, the missing part is a missing corner, so that the sterile individual package culture well plate, the photophobic individual package cover antigen-linked well plate, the ninety-six well plate cover rubber plate film and the ninety-six well plate frame are partially located outside the storage plate at the lower end portion of the end away from the photophobic bag.
In another preferred example, the cross section of the missing part along the direction of the long side of the ninety-six pore plate frame is triangular, quadrangular or trapezoidal.
In another preferred example, the missing part is 2-5cm along the long side direction of the ninety-six pore plate frame.
In another preferred example, the number of the ninety-six pore plate covering the rubber plate film is two.
In another preferred example, the light-shielding independent packaging cover antigen linked-pore plate is positioned below the sterile independent packaging culture pore plate.
In another preferred embodiment, the number of the sterile individually packaged culture well plates is ninety-six and the sterile individually packaged culture well plates are evenly distributed.
In another preferred example, a protruding lifting part is arranged above the object placing plate, so that the object placing plate is lifted out of the box body.
In another preferred example, the lifting portions are two symmetrically arranged.
In another preferred embodiment, the lid is hinged to the body.
It is understood that within the scope of the present invention, the above-mentioned technical features of the present invention and those specifically described below (e.g. in the examples) can be combined with each other to constitute new or preferred technical solutions. Not to be reiterated herein, but to the extent of space.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a right side view of the structure of the present invention;
figure 3 is an example of an aperture plate instrument with one end entirely outside the storage plate.
Figure 4 is an example of an aperture plate instrument with one end partially out of the shelf.
In the figure: 1 box body, 2 standard sample tubes, 3 treatment liquid tubes, 4 cleaning liquid tubes, 5 TMB display liquid tubes, 6 termination liquid tubes, 7 secondary antibody tubes, 8 primary antibody tubes, 9 sterile independent packaging culture pore plates, 10 lightproof independent packaging covering antigen linked pore plates, 11 ninety-six pore plate covering rubber plate films, 12 ninety-six pore plate frames, 13 lightproof bags and 14 missing parts.
Detailed Description
The present inventors have conducted extensive and intensive studies and, as a result, have provided an ELISA kit for detecting TNF- α, a tumor necrosis factor, through a number of screening and testing. The utility model discloses a kit makes things convenient for CAR-T cell's cultivation including a plurality of orifice plate instruments that are used for cell coculture, but these instruments lead to contact between them inseparable in arranging the thing board in with the mode of embedding, are difficult to take out, the utility model discloses a to placing the thing board of putting of instrument is reformed transform, makes but instrument one end can be whole or partial be located put outside the thing board to make the orifice plate instrument more conveniently take out for operating speed. The utility model discloses has accomplished on this basis.
Term(s) for
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
As used herein, the term "about" when used in reference to a specifically recited value means that the value may vary by no more than 1% from the recited value. For example, as used herein, the expression "about 100" includes 99 and 101 and all values in between (e.g., 99.1, 99.2, 99.3, 99.4, etc.).
As used herein, the term "comprising" or "includes" can be open, semi-closed, and closed. In other words, the term also includes "consisting essentially of …," or "consisting of ….
As used herein, the terms "instrument", "well plate instrument" are used interchangeably and refer to the sterile individually packaged culture well plate, photophobic individually packaged cover antigen-linked well plate, ninety-six well plate cover membrane and/or ninety-six well plate rack of the present invention.
Reagent kit
The utility model provides a detect ELISA kit of tumor necrosis factor TNF-alpha, the kit includes:
the box body is provided with an opening at the upper part, and the box cover is used for sealing the opening;
the object placing plate comprises a reagent area, an antibody area and an instrument area, wherein each area is provided with a plurality of object placing grooves with upper openings, and the object placing grooves are used for placing reagent tubes or instruments;
wherein the reagent area is provided with a standard sample tube, a treatment liquid tube, a cleaning liquid tube and a TMB display liquid tube;
the antibody area is provided with a primary antibody tube, a secondary antibody tube and a termination liquid tube;
the instrument area is provided with a sterile independent packaging culture pore plate, a photophobic independent packaging covering antigen linked pore plate, a ninety-six pore plate covering rubber plate film and a ninety-six pore plate frame which are arranged in parallel;
the light-shading bag is arranged on one side of the instrument area of the object placing plate;
the light-proof bag is arranged in the instrument area, and the light-proof bag is arranged in the instrument area and is used for containing the bacteria-free independent packaging culture pore plate, the light-proof independent packaging covering antigen linked pore plate, the ninety-six pore plate covering rubber plate film and the ninety-six pore plate frame.
Generally, the standard tube, the process liquid tube, the wash liquid tube, the TMB indicator liquid tube, the primary antibody tube, the secondary antibody tube and the stopper liquid tube are respectively placed in a cylindrical placement groove.
The sterile independent packaging culture pore plate, the photophobic independent packaging covering antigen linked pore plate, the ninety-six pore plate covering rubber plate film and the ninety-six pore plate frame are placed in the long strip slit-shaped placing grooves which are arranged in parallel.
The utility model discloses to put the one end (being called the left end) that the light-avoiding bag was kept away from in thing board instrument district, detach the part (can be for the complement) for the one end (left end) of orifice plate instrument is whole (from last to down) or partial (only lower part one corner) is located outside putting the thing board, thereby can directly take out the orifice plate instrument or for taking out from the slit from the left end the orifice plate provides the space of rotation and operation, thereby the operation of acceleration.
In another preferred embodiment, the shelf further has a removable complementary piece having a recess for receiving a portion of the instrument located outside the shelf for complementary mounting with the shelf on which the instrument is loaded.
The complementary piece is removed when the instrument is taken out, and the internal instrument can be protected when the reagent box is transported and placed, or the stability of the object placing plate is kept.
In another preferred example, the missing part is a missing corner, so that the sterile individual package culture well plate, the photophobic individual package cover antigen-linked well plate, the ninety-six well plate cover rubber plate film and the ninety-six well plate frame are partially located outside the storage plate at the lower end portion of the end away from the photophobic bag.
The instrument can be pushed out from the unfilled corner part from bottom to top.
In another preferred example, the cross section of the missing part along the direction of the long side of the ninety-six pore plate frame is triangular, quadrangular or trapezoidal.
In another preferred example, the missing part is 2-5cm along the long side direction of the ninety-six pore plate frame.
In another preferred example, the number of the ninety-six pore plate covering the rubber plate film is two.
In another preferred example, the light-shielding independent packaging cover antigen linked-pore plate is positioned below the sterile independent packaging culture pore plate.
In another preferred embodiment, the number of the sterile individually packaged culture well plates is ninety-six and the sterile individually packaged culture well plates are evenly distributed.
In another preferred example, a protruding lifting part is arranged above the object placing plate, so that the object placing plate is lifted out of the box body.
In another preferred example, the lifting portions are two symmetrically arranged.
The utility model discloses a main advantage includes:
the kit of the utility model comprises all reagents required by TNF-alpha detection released by CAR-T cells, is convenient to use, is easy to operate, and can accelerate the operation speed.
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example 1
Referring to fig. 1 to 4, the kit of the present embodiment includes:
a box body 1 with an upper opening and a box cover for sealing the opening;
the object placing plate comprises a reagent area, an antibody area and an instrument area, wherein each area is provided with a plurality of object placing grooves with upper openings, and the object placing grooves are used for placing reagent tubes or instruments;
the reagent area is sequentially provided with a standard sample tube 2, a treatment liquid tube 3 and a cleaning liquid tube 4 from left to right, and the number of the standard sample tube 2, the number of the treatment liquid tube 3 and the number of the cleaning liquid tubes 4 are one; the antibody area is located below the reagent area, a first antibody tube 8 and a second antibody tube 7 are arranged from left to right respectively, and a termination liquid tube 6 is arranged, the instrument area is located below the antibody area, a sterile independent packaging culture pore plate 9 and a light-proof independent packaging cover antigen linked pore plate 10 are sequentially arranged from top to bottom, the light-proof independent packaging cover antigen linked pore plate 10, ninety-six pore plate cover a rubber plate film 11 and ninety-six pore plate frames 12 are arranged, and a light-proof bag 13 is further arranged on the right side of the instrument area.
The end of the placing plate far away from the light-proof bag in the instrument area is provided with a missing part 14, so that the sterile independent packaging culture pore plate, the light-proof independent packaging covering antigen linked pore plate, the ninety-six pore plate covering rubber plate film and the end of the ninety-six pore plate frame far away from the light-proof bag are all or partially positioned outside the placing plate.
Generally, the standard tube, the process liquid tube, the wash liquid tube, the TMB indicator liquid tube, the primary antibody tube, the secondary antibody tube and the stopper liquid tube are respectively placed in a cylindrical placement groove.
The sterile independent packaging culture pore plate, the photophobic independent packaging covering antigen linked pore plate, the ninety-six pore plate covering rubber plate film and the ninety-six pore plate frame are placed in the long strip slit-shaped placing grooves which are arranged in parallel.
The utility model discloses to put the one end (being called the left end) that the light-avoiding bag was kept away from in thing board instrument district, remove the part for the one end (left end) of orifice plate instrument is whole (from last to down) or partial (only lower part one corner) is located outside putting the thing board, thereby can follow the left end and directly take out the orifice plate instrument or for taking out from the slit the orifice plate provides the space of rotation and operation, thereby the operation of acceleration.
An example of an aperture plate instrument with one end entirely outside of the shelf may be seen in fig. 3.
An example of an end of the well plate instrument partially outside the shelf may be as shown in fig. 4.
In another preferred embodiment, the shelf further has a removable complementary piece having a recess for receiving a portion of the instrument located outside the shelf for complementary mounting with the shelf on which the instrument is loaded.
The complementary piece is removed when the instrument is taken out, and the internal instrument can be protected when the reagent box is transported and placed, or the stability of the object placing plate is kept.
In another preferred example, the missing part is a missing corner, so that the sterile individual package culture well plate, the photophobic individual package cover antigen-linked well plate, the ninety-six well plate cover rubber plate film and the ninety-six well plate frame are partially located outside the storage plate at the lower end portion of the end away from the photophobic bag.
The instrument can be pushed out from the unfilled corner part from bottom to top.
In another preferred example, the cross section of the missing part along the direction of the long side of the ninety-six pore plate frame is triangular, quadrangular or trapezoidal.
In another preferred example, the missing part is 2-5cm along the long side direction of the ninety-six pore plate frame.
In another preferred example, the number of the ninety-six orifice plate covering the rubber plate film 11 is two.
In another preferred example, the light-shielding independent packaging cover antigen-linked pore plate 10 is positioned below the sterile independent packaging culture pore plate (9).
In another preferred embodiment, the number of the aseptic individually packaged culture well plates 9 is ninety-six and the sterile individually packaged culture well plates are evenly distributed.
In another preferred example, a protruding lifting part is arranged above the object placing plate, so that the object placing plate is lifted out of the box body.
In another preferred example, the lifting portions are two symmetrically arranged.
The utility model discloses a detect paper ELISA kit of CAR-T cell TNF-alpha release, through the improvement to box body 1, to the factor release of Car-T cell, it is practical succinct more, provide the cell coculture procedure simultaneously and can detect the TNF-alpha release amount of CAR-T cell more scientifically, accurately.
In the description of the present invention, it is to be understood that the terms "coaxial", "bottom", "one end", "top", "middle", "other end", "upper", "one side", "top", "inner", "front", "center", "both ends", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Furthermore, the terms "first", "second", "third", "fourth" are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated, whereby the features defined as "first", "second", "third", "fourth" may explicitly or implicitly include at least one such feature.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "disposed," "connected," "fixed," "screwed" and the like are to be construed broadly, e.g., as meaning fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through an intermediate medium, and may be connected through the inside of two elements or in an interaction relationship between two elements, unless otherwise specifically defined, and the specific meaning of the above terms in the present invention will be understood by those skilled in the art according to specific situations.
All documents mentioned in this application are incorporated by reference into this application as if each were individually incorporated by reference. Furthermore, it should be understood that various changes and modifications of the present invention may be made by those skilled in the art after reading the above teachings of the present invention, and these equivalents also fall within the scope of the appended claims.
Claims (10)
1. An ELISA kit for detecting TNF-alpha, comprising:
the box body is provided with an opening at the upper part, and the box cover is used for sealing the opening;
the object placing plate comprises a reagent area, an antibody area and an instrument area, wherein each area is provided with a plurality of object placing grooves with upper openings, and the object placing grooves are used for placing reagent tubes or instruments;
wherein the reagent area is provided with a standard sample tube, a treatment liquid tube, a cleaning liquid tube and a TMB display liquid tube;
the antibody area is provided with a primary antibody tube, a secondary antibody tube and a termination liquid tube;
the instrument area is provided with a sterile independent packaging culture pore plate, a photophobic independent packaging covering antigen linked pore plate, a ninety-six pore plate covering rubber plate film and a ninety-six pore plate frame which are arranged in parallel;
the light-shading bag is arranged on one side of the instrument area of the object placing plate;
the light-proof bag is arranged in the instrument area, and the light-proof bag is arranged in the instrument area and is used for containing the bacteria-free independent packaging culture pore plate, the light-proof independent packaging covering antigen linked pore plate, the ninety-six pore plate covering rubber plate film and the ninety-six pore plate frame.
2. The ELISA kit of claim 1, wherein said plate further comprises a removable complementary piece, said complementary piece having a recess for receiving a portion of the instrument positioned outside of the plate to be complementarily mounted to the plate with the instrument positioned thereon.
3. The ELISA kit for detecting TNF- α of claim 1, wherein said missing portion is a missing corner such that said sterile individual-packaged culture well plate, photophobic individual package cover antigen-linked well plate, ninety-six well plate cover membrane and ninety-six well plate frame are partially outside of the shelf plate at a lower end thereof distal from the photophobic pouch.
4. The ELISA kit for detecting TNF- α of claim 1, wherein said deletion has a triangular, quadrilateral or trapezoidal cross-section along the length of the ninety-six well plate.
5. The ELISA kit for detecting TNF- α of claim 1, wherein said deletion is 2-5cm along the length of the ninety-six well plate.
6. The ELISA kit for detecting TNF- α of claim 1, wherein the number of the ninety-six well plate covered with the plate membrane is two.
7. The ELISA kit for the detection of tumor necrosis factor TNF- α of claim 1, wherein said sterile individually packaged culture well plates are ninety-six in number and uniformly distributed.
8. The ELISA kit for detecting TNF- α as claimed in claim 1 wherein a raised portion is disposed above the plate to facilitate lifting the plate out of the housing.
9. The ELISA kit for detecting TNF- α of claim 8 wherein said two prompters are symmetrically disposed.
10. The ELISA kit for detecting TNF- α as claimed in claim 1 wherein said cassette lid is hinged to said housing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023069693.XU CN214325779U (en) | 2020-12-18 | 2020-12-18 | ELISA kit for detecting tumor necrosis factor TNF-alpha |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023069693.XU CN214325779U (en) | 2020-12-18 | 2020-12-18 | ELISA kit for detecting tumor necrosis factor TNF-alpha |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214325779U true CN214325779U (en) | 2021-10-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202023069693.XU Active CN214325779U (en) | 2020-12-18 | 2020-12-18 | ELISA kit for detecting tumor necrosis factor TNF-alpha |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214325779U (en) |
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2020
- 2020-12-18 CN CN202023069693.XU patent/CN214325779U/en active Active
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