TWI631337B - Sample inspection device - Google Patents

Abstract

The present invention provides a sample inspection device including: an inspection container having an instrument body, an operation portion, and a reduced diameter distal end portion disposed on a distal end side of the device body, the reduced diameter distal end portion having a distal end; and a test for examining a sample The sheet is placed in the inspection container, and the sample inspection device is operated by the operation portion, and the sample is sucked into the device body via the front end of the reduced diameter front end portion. The inhaled specimen can also be properly discharged.

Description

Sample inspection device Field of invention (Reciprocal reference in related fields)

The present invention claims the benefit of the priority of the specification of Japanese Patent Application No. 2012-238254, the entire disclosure of which is hereby incorporated by reference.

The present invention relates to a specimen inspecting apparatus.

Conventionally, in order to detect a substance to be detected in a sample such as blood, urine, saliva, nasal mucosa, or stool sample, a test piece such as a urine test paper or various clinical examination reagents and devices such as immunochromatography are generally used.

Background of the invention

Japanese Patent Publication No. 2005-515431 (Patent Document 1) discloses a sample container having a test strip container in which a sample collector, a filter, and a test strip are disposed. Japanese Patent Publication No. 2006-194688 (Patent Document 2) discloses a kit for immunochromatography, which comprises a test container containing a sample, and a test device for immunochromatography for inserting a test container from one end side, and a test instrument. With the first determination unit, the test container is in the test device The position corresponding to the first determination unit has a display of the type of the first determination unit. Japanese Laid-Open Patent Publication No. 2009-133757 (Patent Document 3) discloses a test container which is a test container used in a test method including a step of immersing one end portion of a test piece in a sample liquid, and includes a container body and The lid portion that is detachably attached to the opening of the container body is provided with a test piece fixing portion that detachably fixes the other end of the test piece. Japanese Patent Laid-Open No. 2010-91448 (Patent Document 4) discloses a reagent reaction container including a receiving portion having an opening into which a test piece is inserted, a sample receiving portion for accommodating a sample at a bottom portion, and a receiving portion and a sample. An intermediate portion between the accommodating portions, and the reagent reaction container is provided with a protruding rib for preventing the test piece from rotating inside the bottom portion.

Further, there is a technique in which a test piece is immersed in a sample which has been collected in a paper cup or the like, and taken out by a tweezers or the like, and the test paper is pressed against the paper towel to absorb the excess sample, and temporarily placed, and as in Japanese Patent Laid-Open 5-249095 (Patent Document 5) discloses a transparent cover on a test paper, an air chamber at an upper portion thereof, a suction port at a lower portion thereof, a suction port in a liquid, and an air chamber for contacting the liquid with the liquid. Test drug for the inhalation port.

[First technical literature] [Patent Literature]

[Patent Document 1] Special Table 2005-515431

[Patent Document 2] Special Opening 2006-194688

[Patent Document 3] Special Opening 2009-133757

[Patent Document 4] Special Opening 2010-91448

[Patent Document 5] Special Kaiping 5-249095

Summary of invention

When the sample is placed in the test container described in the above Patent Documents 1 to 4, the user must move the sample to the test container with another injection device such as a pipette, and there is a problem that the sample overflows or takes time.

In addition, the test piece is immersed in a sample which has been collected in a paper cup or the like and taken out by a scorpion or the like, and the technique described in Patent Document 5, and the sample can be transferred without using another pipette or the like. In the case of the inspection, the suction port is narrow, and the suction and discharge of the liquid containing the test object cannot be smoothly performed. In addition, when the test piece is exposed to the external environment, when the sample contains an infectious disease cause, there is a risk of contamination such as infection or test piece caused by scattering of the sample and contact with the sample.

SUMMARY OF THE INVENTION An object of the present invention is to provide a specimen inspecting apparatus which can smoothly inject a specimen into a specimen inspecting apparatus directly or appropriately, and which can reduce the risk of contact with a specimen.

The object of the present invention is as follows.

[1] A specimen inspection apparatus comprising: an inspection container having an instrument body, an operation portion, and a reduced diameter distal end portion disposed on a distal end side of the device body, wherein the reduced diameter distal end portion has a distal end; and the sample inspection is performed The test piece is placed in the inspection container, and the sample inspection device sucks the sample into the device body via the front end of the reduced diameter front end portion by the operation of the operation portion.

[2] The sample inspection device according to [1], further comprising a filter for filtering the sample.

[3] The specimen inspection apparatus according to [1] or [2], wherein the test piece is semi-fixed to the inspection container such that the front end of the test piece is in contact with the inner wall of the inspection container.

[4] The specimen inspection apparatus according to any one of [1] to [3] wherein the inspection container is formed of a transparent or translucent material.

[5] The sample inspection device according to any one of [1] to [4] wherein the sample inspection device further includes a lid portion attached to a front end of the reduced diameter front end portion.

[6] The sample testing device according to any one of [1], wherein the test piece is selected from the group consisting of a test piece for immunochromatography, a urine test, and a urine salt concentration. At least one of the test pieces.

[7] The sample testing device according to any one of [1], wherein the test piece is formed by laminating a plurality of test pieces.

According to the sample detecting device of the present invention, the sample can be directly or appropriately discharged into the sample inspecting device and inspected, and the risk of contact with the sample or the like can be reduced.

1‧‧‧Checking device

2‧‧‧Check container

10‧‧‧ Appliance body

10a‧‧‧ outer wall

10b‧‧‧ inner wall

12‧‧‧Migration Department

14‧‧‧ Reduced diameter front end

14a‧‧‧ outer wall

14b‧‧‧ inner wall

16‧‧‧ front end

17‧‧‧ openings

18‧‧‧Operation Department

20‧‧‧Test strips

20a‧‧‧ front end

21‧‧‧Substrate

22‧‧‧ test paper

23‧‧‧sample addition components

24, 26‧‧‧ scale

25‧‧‧Marking holding member

27‧‧‧Chromatographic membrane carrier

28‧‧‧Substrate

27A‧‧‧Decision Department

27B‧‧‧Compare

29‧‧‧absorbing members

30‧‧‧Filter

30a‧‧‧ front end

30b‧‧‧ proximal end member

30c‧‧‧End side members

30d‧‧‧outside of the front end

32‧‧‧ 盖部

32a‧‧ hole

40‧‧‧Check

50‧‧‧test tube

52‧‧‧ urine collection paper cup

Fig. 1 is a schematic view showing a sample testing device according to a first embodiment of the present invention.

Figure 2 is a cross-sectional view taken along line A-A of Figure 1.

Fig. 3 (a) is a front view showing an example of a test piece, and (b) is a side view.

Figure 4 (a) ~ (c) is a sample inspection when the specimen inspection device takes the specimen A schematic view of the device.

5(a) to 5(e) are schematic diagrams showing a modification of the display operation unit.

Fig. 6 is a schematic view showing another example of the apparatus body.

Fig. 7 is a schematic view showing another example of the apparatus body.

Fig. 8 is a schematic view showing another example of a test piece.

Fig. 9(a) shows an example in which two test pieces are attached, and (b) shows a test. A schematic view in which three pieces of the test piece are attached to each other as an example of a cross-sectional triangle.

Fig. 10 is a schematic view showing another example of the arrangement of test pieces.

Fig. 11 is a schematic view showing another example of the arrangement of the filter.

Detailed description of the preferred embodiment

In the present specification, the singular forms (a, an, and) are singular and plural unless they are specifically described in the specification or the context clearly contradicts.

Fig. 1 is a schematic view showing a sample testing device according to a first embodiment of the present invention. The sample inspection device 1 is composed of an inspection container 2 embodied in the form of a dropper in the present embodiment, and a test piece 20 and a filter 30 disposed in the inspection container 2. The capacity of the inspection container 2 is not particularly limited, but is usually 0.5 to 20 ml, preferably 0.5 to 10 ml, and particularly preferably 1 to 5 ml. The inspection container 2 has an operation portion 18 from the proximal end direction toward the distal end direction, a substantially cylindrical instrument body 10, a substantially truncated cone-shaped transition portion 12, and a tapered reduced diameter distal end portion 14 whose diameter gradually decreases toward the distal end direction. And allowing the parts to be in fluid communication with one another (that is, to make it possible for fluids such as air and liquids such as specimens to flow to the parts state). The operation portion 18 has a cylindrical shape in which both ends are rounded into a semi-spherical shape, and is disposed continuously with the luminaire main body 10. The diameter of the operation portion 18 is the same as or larger than the luminaire main body 10. It is preferable that the operation portion 18 has a force that returns to the original state when the user releases the force with a finger or a hand, that is, a restoring force, or is formed of a material having the restoring force.

The specimen is taken for human or non-human animals, and examples of the specimen include blood, urine, nasal mucosa, saliva, pharyngeal throat fluid, and fecal suspension, but are not limited thereto. The specimen is used for inspection in the original (untreated) state, or diluted or treated with a diluent or pretreatment solution. Further, the test substance or the test item in the sample may be any one which can be detected by a known test paper or an equivalent test paper which uses the same principle to detect the test substance. For example, if it is an immunochromatographic test paper, it contains an antigen-producing body. The substance to be reacted is, for example, a cell, a virus, a protein or a polysaccharide of bacteria, a protist or a fungus, but is not limited thereto.

In the inspection container 2, it is preferable to make the test piece 20 in the inspection container 2 at least in part with the chromatographic film carrier 27 (refer to FIG. 3) having the determination portion 27A of the test piece 20 and the comparison portion 27B as will be described later. It is formed of a transparent or translucent material so that the user can make an observation for the determination. Therefore, the inspection container 2 is formed of a transparent plastic such as polyethylene, polypropylene, polystyrene, polyterephthalic acid or the like alone or in combination.

The inspection container 2 is hollow and integrally formed. Therefore, the user can control the amount of the sample entering and leaving the inspection container 2 by adjusting the force applied to the operation portion 18. That is, when the user grips the finger or the hand and presses the operation portion 18, the opening of the front end 16 of the tapered front end portion 14 can be reduced by the pressing force. When the fluid such as the air and the sample is discharged, and the pressed finger or the hand is separated, the fluid can be sucked by the opening 17 of the distal end 16 by the restoring force of the operation portion 18. By adjusting the size of the space inside the operation portion 18, the amount of the sample in the inhalation inspection container 2 can be limited to a fixed amount. For example, when the sample is inhaled into the inspection container 2, the operation unit 18 may be pressed to return a part of the sample to be inhaled to the outside of the examination container 2. The portion of the inspection container 2 other than the opening 17 of the front end 16 is sealed, and the size of the opening 17 is small, so that the amount of fluid in the sample inspection device 1 (and the inspection container 2) can be restricted, and the sample can be reduced. The risk of infection can be suppressed by the exposure of the odor, the scattering of the sample, and the contact of the sample.

An elongated test piece 20 is disposed in the substantially long direction of the instrument body 10 of the inspection container 2 inside the inspection container 2. The sample sucked into the inspection container 2 is supplied to the test piece 20 by the opening 17 of the front end 16 of the reduced diameter front end portion 14. A filter 30 having a substantially cylindrical shape is disposed on the distal end side of the test piece 20 at a distal end portion of the test piece 20, and the filter 30 absorbs the sample and provides a flow of the controlled fluid to the test piece. Filter impurities in the specimen. For example, when the specimen is blood, the filter 30 can separate red blood cells, white blood cells, and platelets from blood to be examined by blood. The filter 30 can also prevent the mixing of the fine particles or the like on the test piece 20 which adversely affect the reflection toward the inspection container 2.

The arrangement of the test piece 20 and the filter 30 will be described in more detail.

Referring to Fig. 2, the luminaire main body 10 has an outer wall 10a and an inner wall 10b. The reduced diameter front end portion 14 has an outer wall 14a and an inner wall 14b, and the diameter of the test piece 20 at the front end 20a and the inner diameter 14b of the reduced diameter front end portion 14 become About the same size It is in contact with the inner wall 14b of the reduced diameter front end portion 14. The contact-test piece 20 can be semi-fixed to the reduced-diameter front end portion 14 (and the inspection container 2), and disposed in a substantially longitudinal direction of the luminaire main body 10 in a state of being separated from the inner wall 10b of the luminaire main body 10. Therefore, the test piece 20 is also semi-fixed when inhaling the sample, and can be quickly immersed in the sample. Further, when the sample is immersed, the test piece 20 can be prevented from being attached to the inner wall 10b of the luminaire main body 10. However, semi-fixed refers to the fixation that is usually fixed when used, but can be taken out by the application of external force.

The filter 30 is frictionally engaged with the inner wall 14b of the reduced diameter front end portion 14 at a position where the filter front end 30a and the inner diameter 14b of the reduced diameter front end portion 14 are approximately the same. The frictional engagement filter 30 is fixed to the reduced diameter distal end portion 14 and the entire cross section of the reduced diameter distal end portion 14 is closed at the fixed portion. Therefore, the filter 30 can efficiently absorb and filter the sample, and the test piece 20 can provide the filtered sample to the test piece 20 instead of the flow rate of rapidly immersing an excessive amount of the sample.

Returning to Fig. 1, the cover portion 32 can also be attached to the inspection container 2. A bottomed hole 32a adapted to inspect the front end 16 of the container 2 is provided in the lid portion 32. By attaching the lid portion 32 to the inspection container 2 before the inspection of the specimen, the specimen inspection device 1 (and the inspection container 2) is sealed, and foreign matter can be prevented from entering the inspection container 2. Further, since the lid portion 32 is attached to the inspection container 2 after the inspection of the specimen, it is possible to prevent the risk of the odor of the specimen from being exposed, the scattering of the specimen, and the contact of the specimen.

Fig. 3(a) is a front view showing an example of the test piece 20, and Fig. 3(b) is a side view. The test piece 20 is an example of a test piece for immunochromatography, and has a substrate 21, a sample adding member 23 disposed on the substrate 21, and a substrate 21 placed in contact with the sample adding member 23. Standard The holding member 25, the chromatographic film carrier 27 disposed on the substrate 21 in contact with the label holding member 25, and the absorbing member 29 disposed on the substrate 21 in contact with the chromatographic film carrier 27 .

The substrate 21 can be formed of various materials such as plastic, paper, and glass. The sample addition member 23 can be formed of various materials such as rayon, glass fiber, and cellulose fiber. The mark holding member 25 is preferably formed of various materials such as glass fibers and cellulose fibers. The chromatographic membrane carrier 27 can be formed by various materials such as nitrocellulose, nylon, and cellulose acetate. The absorbing member 29 can be formed of various materials of cellulose and glass fibers.

The sample addition member 23, the mark holding member 25, the chromatography film carrier 27, and the absorption member 29 are preferably non-woven fabrics or porous bodies, but may be any structure that can stretch the sample by capillary phenomenon. The marker holding member 25 is placed in contact with the sample addition member 23, and can hold the marker substance in the sample that reacts with the measurement target.

The chromatography membrane carrier 27 sequentially forms the determination unit 27A and the control unit 27B on the line from the upstream, and is provided with an immobilized substance capable of capturing each measurement target and the marker substance.

In the present embodiment, the labeling substance of the label holding member 25 is a labeling antibody A which is a gold colloid or the like and which reacts with a specific antigen-antibody reaction for the antigen to be measured. On the other hand, in the determination unit 27A, the capture antibody B which reacts with the specific antigen-antibody reaction to the antigen to be measured is immobilized at a portion different from the portion where the marker antibody A is recognized. Further, an anti-IgG antibody which specifically reacts with the labeled antibody A is immobilized on the control portion 27B.

When the detection method of human hemoglobin is taken as an example, when the human hemoglobin is contained in the sample, the labeled anti-human erythroglobulin antibody remaining in the label holding member 25 recognizes a specific part of the human erythroglobulin and is antigen-antibody The reactions combine to form a complex. Then, the anti-human erythroglobulin antibody immobilized on the determination unit 27A recognizes different parts of the human erythroglobulin and binds by antigen-antibody reaction to capture the complex. By the capture of the complex, a line derived from the marker substance appears in the determination unit 27A, and human erythrocyte globulin can be visually detected.

Further, the anti-IgG antibody immobilized on the control portion 27B recognizes the marker antibody A and binds by the antigen-antibody reaction to capture the marker antibody A. By the capture of the marker antibody A, a line derived from the marker substance appears in the control unit 27B, and it can be visually confirmed that the sample has passed the determination unit 27A to the comparison unit 27B.

As shown in Fig. 4 (a), in the sample inspecting apparatus 1 of the present invention, even when the container sample 40 such as the test tube 50 is small, the liquid can immerse the reduced diameter tip end portion 14 in the sample to take the sample 40. The specimen 40 can also be taken in the case of a container such as a collecting paper cup 52 as shown in Fig. 4(b), and can be directly inspected if it has been taken. In the case where the sample containing the specimen 40 is a large amount as shown in FIG. 4(c), since the specimen inspection apparatus 1 of the present invention can float above the sample, the visual inspection of the judgment section of the user can be ensured. Easy to check.

In the method of manufacturing the sample inspection device 1, for example, two portions of the inspection container 2 are provided, and the test piece 20 and the filter 30 are arbitrarily arranged at a predetermined position on the lower side portion of the inspection container 2, and then the upper side is welded. It is formed by dividing the lower side portion, but is not limited thereto, and may be blow molded or hollow formed.

Next, the operation of the sample testing device 1 according to the first embodiment will be described.

First, the stool sample of the subject is diluted in the pretreatment liquid to prepare a sample. The user presses and releases the operation portion 18 of the inspection container 2, and inhales the sample in the inspection container 2. Here, the sample to be inhaled collides with the front end 30a of the filter 30, and the rapid flow of the sample is prevented from directly reaching the test piece 20, and the sample is filtered by the filter 30. The sample that has passed through the filter 30 accumulates in the space between the filter 30 and the test piece 20 opposite to the filter 30, and reaches the front end 20a of the test piece 20. The sample usually reaches the same level as the sample adding member 23, but does not reach the chromatographic film carrier 27 having the determining unit 27A and the control unit 27B so as not to interfere with the determination.

In this state, when placed for about 10 to 20 minutes, the sample is sequentially moved by the capillary addition phenomenon to the sample addition member 23, the marker holding member 25, the chromatography membrane carrier 27, and the absorption member 29. When the sample passes the identification holding member 25, the identification substance held by the identification holding member 25 is dissolved in the diluent or the pretreatment liquid. When the human red blood globulin is contained in the sample, the line appears in the determining portion 27A by the above action, and the line appears in the control portion 27B regardless of the presence or absence of the human erythroglobulin.

When the inspection is completed, the lid portion 32 is attached to the inspection container 2 to seal the device 1, and the specimen inspection device 1 is disposed.

Next, the sample testing device 1 according to the first embodiment described above The effects are explained below.

(1) The sample testing device 1 of the above embodiment includes the test container 2 and the test piece 20, and the sample is sucked into the device body 2 through the front end 16 of the reduced diameter front end portion 14 by the operation of the operation portion 18, the test container 2 having The luminaire main body 10, the operation unit 18, and the reduced diameter distal end portion 14 disposed on the distal end side of the luminaire main body 10, and the reduced diameter distal end portion 14 has a distal end 16, and the test piece 20 is placed in the examination container 2 for examination of the specimen. Test piece 20.

With this configuration, since the diameter of the distal end 16 is small and the portion of the examination container 2 other than the distal end 16 is sealed, it can be inhaled by the operation of the operation portion 18 in the specimen inspection device 1 (and the inspection container 2). A fixed amount of the specimen. Further, after the specimen is brought into contact with the test piece 20 for a fixed period of time, the specimen can be easily discharged by the operation of the operation portion 18. Further, it is possible to reduce the risk of the odor of the specimen, the scattering of the specimen, and the contact of the specimen, and the risk of infection can be suppressed.

(2) At the position of the inner wall 14b of the reduced diameter distal end portion 14, the filter 30 for filtering the specimen is fixed so as to close the entire cross section of the reduced diameter distal end portion 14.

According to this configuration, not only the impurities in the sample can be filtered, but also the rapid flow of the sample can be prevented from directly reaching the test piece 20.

(3) The test piece 20 is semi-fixed to the inspection container 2 by bringing the front end 20a of the test piece 20 into contact with the inner wall 14b of the reduced diameter front end portion 14 of the inspection container 2.

With this configuration, the sample sucked into the inspection container 2 is quickly immersed in the test piece 20. Further, it is also possible to suppress the test piece 20 from being attached to the inner wall 10b of the luminaire main body 10 at the time of immersion of the sample.

(4) The inspection container 2 is formed of a transparent or translucent material.

With this configuration, it is possible to easily observe the test piece 20 used by the user.

(5) The sample inspection device 1 further has a lid portion 32 attached to the front end 16 of the reduced diameter front end portion 14.

According to this configuration, by attaching the lid portion 32 to the inspection container 2 before the inspection of the specimen, the inspection container 2 can be sealed to prevent foreign matter from entering the inspection container 2. Further, by attaching the lid portion 32 to the inspection container 2 after the inspection of the specimen, it is possible to prevent the risk of the odor of the specimen from being exposed, the scattering of the specimen, and the contact of the specimen.

(6) The sample inspecting apparatus 1 can perform the test as long as there is a fixed amount of the multi-tube which is not related to the object to be inspected, and in the case where the number of the test samples is large, since it can float on the sample, it can be easily observed.

Heretofore, the present invention has been described by way of the first embodiment, but the present invention is not limited thereto, and various modifications can be made as follows.

○ As shown in FIGS. 5( a ) to 5 ( e ), the operation unit 18 can be modified into various modifications. Fig. 5 (a) shows the operation portion 18 in the shape of a bellows. The operation unit 18 can be restored, that is, the operation unit 18 can reciprocate in the direction indicated by the arrow. Fig. 5(b) is a front view showing the operation portion 18 of the flat hexahedron having a rounded corner, and Fig. 5(c) is a side view. Fig. 5(d) is the operation portion 18 having a larger width as it approaches the proximal end direction. The operation unit 18 of FIGS. 5(b) to (d) is a flat type in which the front surface area is larger than the side surface area. The discharge and suction of the air, the sample, and the like can be performed by the configuration of Figs. 5(a) to (d). Further, as shown in FIG. 5(e), the operation portion 18 may be a portion of the luminaire main body 10 that is enlarged toward the proximal end direction. as long as The ware body 10 is provided with a sufficient space for discharging and sucking the fluid, and the luminaire main body 10 can also serve as the operation portion 18. In this case, the operation portion 18 is also in fluid communication with the luminaire body 10.

○ The operation unit 18 and the luminaire main body 10 may be connected to each other through a separate portion such as a transition portion. In this case, the operating portion 18 is also in fluid communication with the appliance body 10.

○ The transition portion 12 may be omitted, and the luminaire main body 10 that is tapered toward the distal end side may be directly connected to the reduced diameter distal end portion 14.

○ The diameter-reducing distal end portion 14 does not need to have a tapered shape as long as the diameter becomes smaller than the device body 10. For example, the reduced diameter front end portion 14 may be a member having a smaller diameter than the device body 10 and having a fixed diameter.

○ The front end 16 of the reduced diameter front end portion 14 of Fig. 1 may not be provided with the opening 17 at the beginning. The inspection container 2 is formed of a material having low moisture permeability before use, and a desiccant is sealed in the inside of the inspection container 2 to seal the sample inspection device 1 to improve storage stability. Also, at the time of inspection, the front end 16 may be cut or the opening 17 may be opened by another method.

○ By sealing the inside of the inspection container 2 with the desiccant and attaching the lid portion 32, the sample inspection device 1 can be sealed to improve storage stability. Just remove the cover 32 when inspecting.

○As shown in FIG. 6, the positions A and B indicating the types of the test piece 27A and the control unit 27B may be provided at positions corresponding to the test piece determination unit 27A and the comparison unit 27B on the apparatus main body 10. . It is indicated that A and B may be attached by attaching a label to the inspection container 2, or may be attached to the inspection container 2 by other methods such as direct printing of the inspection container 2. Take this The user can accurately recognize the type of the line of the determination unit 27A and the comparison unit 27B that appear.

○ As shown in Fig. 7, the scales 24, 26 may be provided at positions corresponding to the specific positions of the test piece 10 of the instrument body 10. The scales 24, 26 may be directly machined to the device body 10, or may be indicia or labels. In the example of Fig. 7, the scale 24 is the position of the sample of the sample and the boundary between the sample adding member 23 and the mark holding member 25 is set at the same position, and the scale 26 is the mark holding member 25 and the chromatography film carrier 27 The boundary between them is set at the same position. The user confirms the height of the sample in the inhalation inspection container 2 on the scales 24 and 26, and can appropriately discharge the sample or the like, and appropriately adjust the amount of the sample in the inspection container 2 so that the inhaled sample does not reach the determination portion 27 .

○ The determination can also be read by the reader of the shape of the sample testing device 1.

○ The antigen to be measured is not particularly limited as long as it is an antigen-antibody reaction, and examples of the human erythrocyte globulin antigen include antigens such as cells such as bacteria, protists, and fungi, viruses, proteins, and polysaccharides.

○ When the target is an antigen, and the target is an antigen, the labeled substance and the immobilized substance of the determination unit 27A are antibodies that react with the antigen, and the measurement is reversed. In the case of an antibody, the labeling substance and the immobilized substance of the determining unit 27A are antigens that react with the antibody in an antigen-antibody reaction.

○ In addition to the gold colloid, the marking substance of the mark holding member 25 may be a gold colloid, a latex particle, a dye molecule or an enzyme other than gold. To identify.

○ The determination unit 27A may have two or more. In this case, the labeling substance of the label holding member 25 is a mixture of two or more antigens or antibodies, and the determining unit has a number corresponding to the labeling substance.

○ In the immunochromatography method, when the enzyme-labeled antibody is used in the conjugate bound to the sample, a fixed amount of the sample is stretched in the first stage in the first stage, and the sample can be directly inspected in the second stage. The device 1 moves in the enzyme chromogenic substrate liquid, and the enzyme chromogenic substrate is extended in the same test piece, and simple and sensitive detection can be performed. The combination of the labeling enzyme and the enzyme chromogenic substrate may, for example, be alkaline phosphatase or 5-bromo-4-chloro-3-indolyl phosphate disodium salt, but a known combination is not limited thereto.

○ The length of the test piece 20 is not particularly limited, and may be a length that can perform necessary inspection.

○ The test piece 20 is not limited to the immersion immunochromatographic test paper, and may be a test paper using an antigen-antibody reaction, any known test paper, or an equivalent test paper using the same principle as the known test substance. For example, as shown in FIG. 8, the test piece 20 may be a urine test piece in which a plurality of urine test materials or a plurality of test papers 22 are disposed on a substrate 28 formed of plastic or the like. In the case of one test strip 22 corresponding to one test item, at least one of white blood cells, urobilinogen, occult blood, bilirubin, ketone body, glucose, protein, pH, nitrite and specific gravity can be inspected. project. The other test piece 20 includes a test piece in which a plurality of test papers 22 are disposed on the substrate 28 to measure the salt concentration in the urine. However, examples of urine test samples can be exemplified by Rongyan Chemical Co., Ltd. The product name of the company is a uropaper (registered trademark) series, and an example of a test piece for measuring the urine salt concentration is, for example, Saltpaper (registered trademark) manufactured by Eiken Chemical Co., Ltd. According to this configuration, the present invention can easily and quickly perform inspection items that are frequently used for inspection.

○ As shown in FIG. 9 , the test piece 20 may be one in which the two types of test pieces 20 are bonded to each other with the inspection surface facing the surface, or the three test pieces may have a triangular cross section. They are made to fit each other. Further, four or more test pieces may be used. With such a configuration, it is possible to check different items at the same time.

○ As shown in FIG. 10, the test piece 20 is obtained by cutting the two corners of the front end side of the test piece 20, and matching the oblique side of the cut side with the inclination angle of the inner wall 14b of the reduced diameter front end portion 14, thereby strengthening and The front end 20a has substantially the same width and contact with the inner wall 14b of the reduced diameter front end portion 14. Further, the two corners of the front end side of the test piece 20 are not cut, and the two corners of the front end side of the test piece 20 are bent to match the inclination angle of the inner wall 14b (not shown).

○ The test piece 20 may not contact the inner wall 14b of the reduced diameter front end portion 14 and contact the inner wall of the other member of the inspection container 2. For example, the test piece 20 may also contact the inner wall of the transition portion 12 or the inner wall 10b of the luminaire body 10.

○ The test piece 20 may not be semi-fixed to the inner wall 14b of the reduced-diameter front end portion 14 so as to be freely movable in the inspection container 2. In this case, it is preferable that the test piece 20 is movable so that the front end 20a of the test piece 20 exists in the reduced diameter front end portion 14 at the time of inspection.

○ The filter 30 is not limited to a substantially cylindrical shape, and may have any shape. As shown in FIG. 11, the filter 30 can also be composed of a large-diameter base end member. 30b and a distal end side member 30c having a smaller diameter than the proximal end side member 30b extending from the center of the front end side of the proximal end side member 30b. In this case, the outer peripheral edge 30d of the front end side of the proximal end side member 30b is in contact with the inner wall 14b of the reduced diameter distal end portion 14.

○ The filter 30 may be disposed at any position between the front end 16 of the reduced diameter distal end portion 14 and the test paper 20. For example, the filter 30 may also be disposed at the front end or the transition portion 12 of the appliance body 10.

○ The filter 30 can also be in contact with a plurality of locations along the length of the inspection container 2.

○ The filter 30 can also be omitted.

The present invention will be more specifically described by the following examples, but it is obvious that the invention is not limited thereto.

Example Example

Insert a human hemoglobin detection immunochromatographic test strip (manufactured by Eiken Co., Ltd., trade name OC-Light) into a translucent dropper made of polyethylene (manufactured by ART Corporation, No. 88337) having a diameter of 6.5 mm. The test shall be provided in a state in which the test strip is not fixed to the dropper.

Negative standard solution of human red blood globulin (red blood globulin concentration: 0 ng / mL, HEPES buffer) and positive standard solution (red blood globulin concentration: 450 ng / mL, HEPES buffer) were measured 10 times.

During the operation, when the dropper is pressed, the liquid is introduced into the dropper by the restoring force, and the test strip is immersed. After the suction of the liquid, the test strip was visually measured after 5 minutes. The test results are obtained by the line of the control unit and the control unit. It was confirmed by color development that the negative standard solution was all (-), and the positive standard solution was all (+).

In addition, as for the amount of the suction liquid, the reduction of the standard solution before and after the attraction was determined, and the negative standard solution was 0.52±0.03 mL (mean ± SD), and the positive standard solution was 0.51 ± 0.03 mL (mean ± SD). The reproducibility of the amount of suction liquid is good.

Industrial availability

Since the novel sample inspecting apparatus of the present invention has a small diameter at the front end of the tapered distal end portion of the inhaled sample, it is very useful in reducing the risk of contact with the specimen or the like.

Claims (9)

A specimen inspection apparatus includes: an inspection container having an apparatus body, an operation unit that can adjust a size of a space inside by pressing or releasing the pressing, and a reduced diameter front end portion disposed on a distal end side of the device body The front end portion of the reduced diameter has a distal end, and the instrument body, the operation portion, and the reduced diameter distal end portion are integrally formed, and the portion other than the opening of the distal end is sealed; and the test piece for the sample inspection is placed in the test container. The specimen inspecting apparatus according to claim 1, wherein the specimen inspecting means sucks the specimen into the apparatus main body via the front end of the reduced diameter distal end portion by the operation of the operation portion. The sample inspecting device of claim 1 or 2 further has a filter for filtering the sample. The specimen inspection apparatus of claim 1, wherein the test piece is semi-fixed to the inspection container by contacting the front end of the test piece with the inner wall of the inspection container. The specimen inspection device of claim 1, wherein the inspection container is formed of a transparent or translucent material. The specimen inspection device of claim 1, wherein the specimen inspection device further has a lid portion attached to a front end of the reduced diameter front end portion. The specimen inspection apparatus according to claim 1, wherein the test piece is at least one selected from the group consisting of a test piece for immunochromatography, a urine test piece, and a test piece for measuring a urine salt concentration. The sample inspection device of claim 1, wherein the aforementioned test piece is a plural The test pieces are laminated. A sample inspection device includes: an inspection container having an instrument body, an operation portion, and a reduced diameter distal end portion disposed on a distal end side of the device body, and the reduced diameter distal end portion has a distal end, and a test for examining the sample The sheet is placed in the inspection container, and the integrally formed device body, the operation portion, and the reduced diameter front end portion constitute a sealed inspection container.