CN214300138U

CN214300138U - Buffalo excrement DNA draws kit

Info

Publication number: CN214300138U
Application number: CN202120037337.5U
Authority: CN
Inventors: 李志鹏; 刘庆友; 黄孔威; 冯彤; 羊玉凤
Original assignee: Guangxi University
Current assignee: Guangxi University
Priority date: 2021-01-07
Filing date: 2021-01-07
Publication date: 2021-09-28
Anticipated expiration: 2031-01-07

Abstract

The utility model discloses a buffalo excrement and urine DNA draws kit relates to kit technical field. Including organism, box body, sampling mechanism and draw the mechanism, the fixed box body that is provided with in bottom of organism, the top activity of box body is provided with sampling mechanism. Through setting up the inserted block, make it effectively pound buffalo excrement and urine, and then take a sample through the sampling spoon, and place it on the separating layer, and then prevent that faecal peculiar smell from giving off through the spacer, and through its separation, avoid faecal four to spatter the adhesion, the clean health of device has effectively been improved, and then add reagent to the excrement and urine of placing it in, and carry out solid-liquid separation through the separating layer, simultaneously through the effect of hang plate, with another part in its leading-in box body of liquid part, and then the rubber sleeve passes through the effect of atmospheric pressure passageway, make the plastics burette operation, the reagent of being convenient for adds the absorption, and then effective convenient device is to the extraction of DNA, and faecal validity has been ensured, and then the practicality that the device used has been strengthened.

Description

Buffalo excrement DNA draws kit

Technical Field

The utility model relates to a kit technical field specifically is a buffalo excrement and urine DNA draws kit.

Background

The DNA extraction kit is constructed according to a benzyl chloride method, and can extract genomic DNA from a sample, wherein the benzyl chloride has the characteristic of being capable of benzylating hydroxyl groups in cell walls containing cellulose and the like so as to destroy the cell walls, the product utilizes the characteristic of the benzyl chloride, after freezing and melting a plant sample, the plant sample is pressed on the walls of Microtube for a plurality of times by using the Tip of a pipe Tip so as to destroy the cell walls, compared with the traditional method, the method omits the complicated steps of liquid nitrogen grinding and the like, is favorable for processing a large number of samples at one time, and the product is improved, the heat treatment time is only 15 minutes, the time from the beginning of an experiment to the recovery of a water phase is only 30 minutes, and the obtained genomic DNA can be subjected to PCR amplification, enzyme restriction treatment and the like.

Present buffalo excrement and urine DNA draws collects the uncovered plastic box of disposable stereoplasm that adopts the conventionality, but its uses, need place into DNA extraction kit through excrement and urine sampling device with the sample of excrement and urine collection, and then lead to its faecal time of touching long, easily improve DNA and draw degree of difficulty and accuracy, and deepened the loaded down with trivial details degree of operation, it uses inconveniently, the seal of current device is relatively poor simultaneously, make its faecal peculiar smell distribute, and inside easily receives the influence of bacterium in the air, and then the length of time and the accuracy of the extraction of improvement device, and the structure setting of device is too simple, make its excrement and urine adhesion inside and outside the device, and then lead to its sanitary effect relatively poor, personnel's of not being convenient for operation is used, therefore, provide a buffalo excrement and urine DNA and draw kit and solve above-mentioned problem.

SUMMERY OF THE UTILITY MODEL

The utility model provides a buffalo excrement and urine DNA draws kit possesses the advantage that draws convenience, leakproofness well reach clean health to solve general kit and do not possess the problem of drawing convenience, leakproofness well reach clean health.

For the realization possess draw convenient, the leakproofness well reaches clean sanitary purpose, the utility model provides a following technical scheme: a buffalo excrement DNA extraction kit comprises a machine body, a box body, a sampling mechanism and an extraction mechanism, wherein the box body is fixedly arranged at the bottom of the machine body, the sampling mechanism is movably arranged at the top of the box body, and the extraction mechanism is movably arranged in the box body;

the box body includes lid, observation window, scale mark, baffle, filter screen, separation layer, spacer and places the hole, the top activity of box body is provided with the lid, the observation window has been seted up in the front of box body, the fixed scale mark that is provided with in front of box body, the fixed baffle that is provided with of inner diapire of box body, the fixed filter screen that is provided with in inside of baffle, the inside wall of box body is by fixed spacer and the separation layer of having set gradually under the top, the hole of placing has been seted up at the top of lid.

As a preferred technical scheme of the utility model, sampling mechanism's bottom is provided with the sample spoon, and the one end of sampling spoon is provided with the inserted block, and the outer wall of sample spoon is provided with the connecting piece, and sampling mechanism passes through connecting piece and lid swing joint.

As an optimal technical scheme of the utility model, the top of drawing the mechanism is provided with the rubber sleeve, and the bottom of rubber sleeve is provided with the atmospheric pressure passageway, and the bottom of atmospheric pressure passageway is provided with the plastics burette.

As a preferred technical scheme of the utility model, the thread groove has been seted up to the outer wall of atmospheric pressure passageway, and the atmospheric pressure passageway passes through thread groove and lid threaded connection.

As a preferred technical scheme of the utility model, the central line of baffle and the central line coincidence of box body, and the inside of box body is through two minutes deposits spaces of baffle separation department, and is provided with sampling mechanism respectively in two spaces and draws the mechanism, and the interior diapire of box body is the inclined bottom plate setting.

As a preferred technical scheme of the utility model, the spacer will divide and deposit the space separation and become isolation region and active area, and the isolation region setting is in the top of active area, and the spacer cooperates sealed grafting with the sampling spoon.

Compared with the prior art, the utility model provides a buffalo excrement and urine DNA draws kit possesses following beneficial effect:

1. this buffalo excrement and urine DNA draws kit, through setting up the inserted block, make it effectively pound buffalo excrement and urine to pieces, and then take a sample through the sampling spoon, and place it on the separating layer, and then prevent that faecal peculiar smell from giving off through the spacer, and through its separation, avoid faecal four to spatter the adhesion, the clean health of device has effectively been improved, and add reagent to the excrement and urine of placing it in, and carry out solid-liquid separation through the separating layer, simultaneously through the effect of hang plate, with another part in the leading-in box body of its liquid part, and then the rubber sleeve passes through the effect of atmospheric pressure passageway, make the operation of plastics burette, the reagent of being convenient for adds the absorption, and then effective convenient device is to the extraction of DNA, and faecal validity has been ensured, and then the practicality that the device used has been strengthened.

2. This buffalo excrement and urine DNA draws kit, through setting up the baffle, make the inside group of its box body separate the separation space, and make excrement and urine carry out solid liquid state branch deposit, and then be convenient for draw of the follow-up DNA of device, and deposit through dividing and set up and draw, the accuracy nature of device use has been ensured, the device is many through thread groove connecting device each part simultaneously, and through the sealed pad that the lid inner wall set up, the inside leakproofness of device has effectively been ensured, fecal ageing is stabilized, and the setting of multinomial structure, the functionality of device has effectively been strengthened, and convenient the use operation of device, the usability of device has been promoted.

Drawings

Fig. 1 is a schematic structural view of the present invention;

fig. 2 is a front sectional view of the present invention;

fig. 3 is an enlarged schematic structural diagram of a in fig. 2 according to the present invention.

In the figure: 1. a body; 2. a box body; 201. a box cover; 202. an observation window; 203. scale lines; 204. a partition plate; 205. filtering with a screen; 206. a separation layer; 207. a spacer sleeve; 208. placing holes; 3. a sampling mechanism; 301. sampling a spoon; 302. inserting a block; 303. a connecting member; 4. an extraction mechanism; 401. a rubber sleeve; 402. an air pressure channel; 403. a plastic dropper; 404. a thread groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-3, the utility model discloses a buffalo excrement and urine DNA draws kit, including organism 1, box body 2, sampling mechanism 3 and draw mechanism 4, the fixed box body 2 that is provided with in bottom of organism 1, the top activity of box body 2 is provided with sampling mechanism 3, the inside activity of box body 2 is provided with draws mechanism 4.

The box body 2 comprises a box cover 201, an observation window 202, scale marks 203, a partition plate 204, a filter screen 205, a separation layer 206, a spacer 207 and a placing hole 208, the box cover 201 is movably arranged at the top of the box body 2, the observation window 202 is arranged on the front face of the box body 2, the scale marks 203 are fixedly arranged on the front face of the box body 2, the partition plate 204 is fixedly arranged on the inner bottom wall of the box body 2, the filter screen 205 is fixedly arranged inside the partition plate 204, the spacer 207 and the separation layer 206 are sequentially and fixedly arranged on the inner side wall of the box body 2 from top to bottom, and the placing hole 208 is arranged at the top of the box cover 201.

Specifically, the bottom of sampling mechanism 3 is provided with sampling spoon 301, and the one end of sampling spoon 301 is provided with inserted block 302, and the outer wall of sampling spoon 301 is provided with connecting piece 303, and sampling mechanism 3 passes through connecting piece 303 and lid 201 swing joint.

In this embodiment for through inserted block 302, make it effectively pound buffalo excrement and urine to pieces, and then take a sample through sampling spoon 301, simultaneously through the effect of connecting piece 303, effectively place sampling mechanism 3 in the inside of lid 201, and then enlarge it and place the mouth, avoid faecal adhesion, improve the sanitary degree of device.

Specifically, the top of the extraction mechanism 4 is provided with a rubber sleeve 401, the bottom of the rubber sleeve 401 is provided with an air pressure channel 402, and the bottom of the air pressure channel 402 is provided with a plastic dropper 403.

In this embodiment, make the device through extrusion rubber sleeve 401 to through the effect of atmospheric pressure passageway 402, make plastics burette 403 operation, the DNA of being convenient for draws the interpolation of reagent and absorbs, and then effectively makes things convenient for the device to the extraction of DNA, and ensured faecal validity, and then strengthened the practicality that the device used.

Specifically, the outer wall of the air pressure channel 402 is provided with a thread groove 404, and the air pressure channel 402 is in threaded connection with the box cover 201 through the thread groove 404.

In this embodiment for atmospheric pressure passageway 402 passes through the effect of thread groove 404, and the convenient installation that drives extraction mechanism 4 is used, simultaneously through its threaded connection, prolongs export air contact distance, and then the inside leakproofness of hoisting device to faecal timeliness has been stabilized, and then has ensured the accuracy that device DNA drawed.

Specifically, the central line of baffle 204 and the central line coincidence of box body 2, and two branch deposit spaces of baffle 204 separation department are passed through to the inside of box body 2, and are provided with sampling mechanism 3 and draw mechanism 4 in two spaces respectively, and the interior diapire of box body 2 is the inclined bottom plate setting.

In this embodiment for the device passes through baffle 204, makes the inside group of its box body 2 separate the separation space, and makes excrement and urine carry out solid-liquid state branch and deposit, and then carries out the water conservancy diversion through the effect of inclined floor, has effectively made convenient the extraction of the follow-up DNA of device, and deposits the setting through dividing and draw, has ensured the accuracy nature that the device used.

Specifically, the spacer 207 separates the separate storage space into an isolation region and an effective region, the isolation region is arranged above the effective region, and the spacer 207 is matched with the sampling spoon 301 for sealing insertion.

In this embodiment, the leakproofness that effectively ensures the device and uses, stabilising arrangement's result of use avoids it to appear polluting, influences the accuracy that the follow-up DNA of sample drawed to through the separation setting of isolation region and active area, avoided its excrement and urine to vibrate in the transportation in disorder and spatter, make the inner wall of its adhesion box body 2, the follow-up use of being not convenient for, and ensured the clean health of its device.

The utility model discloses a theory of operation and use flow: when using, through inserted block 302, make it effectively pound buffalo excrement and urine, and then take a sample through sampling spoon 301, and place it on separation layer 206, and then prevent that faecal peculiar smell from giving off through spacer 207, and through its separation, avoid faecal four to spatter the adhesion, the clean health of device has effectively been improved, and then add reagent to the excrement and urine of placing it, and carry out solid-liquid separation through separation layer 206, simultaneously through the effect of inclined bottom plate, with another part in its leading-in box body 2 of liquid part, and then the effect that rubber sleeve 401 passes through atmospheric pressure passageway 402, make plastics burette 403 operate, the reagent of being convenient for adds the absorption, and then effective convenient device is to the extraction of DNA, and faecal validity has been ensured, accomplish the operation to the device through the aforesaid.

To sum up, this buffalo excrement and urine DNA draws kit, through setting up baffle 204, make the inside group of its box body 2 separate the separation space, and make excrement and urine carry out solid liquid state branch deposit, and then be convenient for draw of the follow-up DNA of device, and set up through dividing and draw, the accuracy nature of device use has been ensured, the device is many simultaneously through thread groove 404 connecting device each part, and the sealed pad that sets up through lid 201 inner wall, the inside leakproofness of device has effectively been ensured, fecal timeliness has been stabilized, and the setting of multinomial structure, the functionality of device has effectively been strengthened, and convenient the use operation of device, the usability of device has been promoted.

It should be noted that, in this document, terms such as "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a buffalo excrement and urine DNA draws kit, includes organism (1), box body (2), sampling mechanism (3) and draws mechanism (4), its characterized in that: a box body (2) is fixedly arranged at the bottom of the machine body (1), a sampling mechanism (3) is movably arranged at the top of the box body (2), and an extraction mechanism (4) is movably arranged in the box body (2);

the box body (2) comprises a box cover (201), an observation window (202), scale marks (203), a partition plate (204), a filter screen (205), a separation layer (206), a separation sleeve (207) and a placing hole (208), the box cover (201) is movably arranged at the top of the box body (2), the observation window (202) is arranged on the front face of the box body (2), the scale marks (203) are fixedly arranged on the front face of the box body (2), the partition plate (204) is fixedly arranged on the inner bottom wall of the box body (2), the filter screen (205) is fixedly arranged inside the partition plate (204), the separation sleeve (207) and the separation layer (206) are fixedly arranged on the inner side wall of the box body (2) from top to bottom in sequence, and the placing hole (208) is arranged at the top of the box cover (201).

2. The buffalo excrement DNA extraction kit according to claim 1, characterized in that: the bottom of sampling mechanism (3) is provided with sampling spoon (301), and the one end of sampling spoon (301) is provided with inserted block (302), and the outer wall of sampling spoon (301) is provided with connecting piece (303), and sampling mechanism (3) pass through connecting piece (303) and lid (201) swing joint.

3. The buffalo excrement DNA extraction kit according to claim 1, characterized in that: the top of the extraction mechanism (4) is provided with a rubber sleeve (401), the bottom of the rubber sleeve (401) is provided with an air pressure channel (402), and the bottom of the air pressure channel (402) is provided with a plastic dropper (403).

4. The buffalo excrement DNA extraction kit according to claim 3, characterized in that: the outer wall of the air pressure channel (402) is provided with a thread groove (404), and the air pressure channel (402) is in threaded connection with the box cover (201) through the thread groove (404).

5. The buffalo excrement DNA extraction kit according to claim 1, characterized in that: the central line of baffle (204) and the central line coincidence of box body (2), and the inside of box body (2) is passed through baffle (204) separation and is gone out two branch and deposit the space, and is provided with sampling mechanism (3) and draws mechanism (4) in two spaces respectively, and the interior diapire of box body (2) is the inclined bottom plate setting.

6. The buffalo excrement DNA extraction kit according to claim 5, characterized in that: the separation and storage space is divided into an isolation area and an effective area by the spacer sleeve (207), the isolation area is arranged above the effective area, and the spacer sleeve (207) is matched with the sampling spoon (301) for sealing insertion.