CN214201277U - Sample target processing system of matrix-assisted laser desorption ionization time-of-flight mass spectrometer - Google Patents
Sample target processing system of matrix-assisted laser desorption ionization time-of-flight mass spectrometer Download PDFInfo
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- CN214201277U CN214201277U CN202022956097.7U CN202022956097U CN214201277U CN 214201277 U CN214201277 U CN 214201277U CN 202022956097 U CN202022956097 U CN 202022956097U CN 214201277 U CN214201277 U CN 214201277U
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Abstract
The utility model discloses a sample target processing system of a matrix-assisted laser desorption ionization time-of-flight mass spectrometer, which comprises a vacuum pumping system, a vacuum sample introduction chamber provided with a cabin door, and a vacuum drying chamber used for drying a sample target to be tested; a moving part for bearing a sample target groove is arranged in the vacuum sample introduction chamber, and the sample target groove is used for bearing a sample target to be detected; a transition chamber is arranged at the sample inlet of the vacuum sample injection chamber and is used for enabling the sample target slot, the sample inlet and the cabin door to enclose to form a closed cavity when the sample target slot moves to the sample inlet; an exhaust filter is arranged in a vacuumizing gas path of the vacuum drying chamber and is used for filtering harmful impurities in the vacuum drying chamber; the sample inlet is communicated with the atmosphere through a valve. The utility model discloses the advantage lies in need not to wait for the sample target drying and can accomplish the mass spectrometry for mass spectrometry detection work step shortens greatly, has avoided the biological safety problem in the drying process simultaneously.
Description
Technical Field
The utility model relates to a matrix-assisted laser desorption ionization time-of-flight mass spectrometer especially relates to matrix-assisted laser desorption ionization time-of-flight mass spectrometer sample target processing system.
Background
Matrix Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometer (MALDI-TOF MS) is a novel soft Ionization biological Mass spectrum developed in recent years, and has the advantages Of high sensitivity, high accuracy, high resolution and the like, and is widely used for genomics and proteomics research.
In the identification of the microbial biomass spectrum, the pretreatment work of a sample to be detected is a key factor influencing the success or failure of an identification result, and the pretreatment sample adding and spotting operation is an important operation influencing the success or failure of the pretreatment of the sample. After the pretreatment of the sample of the microbial mass spectrum is finished, the sample can enter a mass spectrometer for identifying the microbial mass spectrum after being dried on the sample target. The detection steps are as follows: 1. picking a sample to a target plate; 2. adding a matrix solution; 3. waiting for the sample added with the matrix liquid to be dried; 4. entering a MALDI-TOF MS vacuum chamber for mass spectrum detection. At present, the drying mode of the matrix liquid sample is mainly as follows: the sample target with the undried sample is allowed to air-dry by itself, or is dried by heating the sample target with the undried sample. The self-air-drying mode takes longer time, and the working efficiency of the microorganism mass spectrum identification is reduced; the manner of heat drying will cause the sample to decompose, affecting the stability of the sample. Meanwhile, the two drying modes are both in an open environment, and the biological safety problem is easily caused in the drying process.
Disclosure of Invention
An object of the utility model is to provide a matrix-assisted laser desorption ionization time of flight mass spectrograph sample target processing system to realize rapid stabilization dry and do not influence sample stability, dry environment seals, avoids the biological safety problem.
In order to achieve the above purpose, the utility model adopts the following technical proposal:
the utility model discloses a matrix-assisted laser desorption ionization time-of-flight mass spectrometer sample target processing system, which comprises a vacuum pumping system, a vacuum sample introduction chamber provided with a cabin door, and a vacuum drying chamber for drying a sample target to be tested; a moving part for bearing a sample target groove is arranged in the vacuum sample introduction chamber, and the sample target groove is used for bearing a sample target to be detected; a transition chamber is arranged at a sample inlet of the vacuum sample introduction chamber and is used for enabling a sample target slot, the sample inlet and the cabin door to enclose to form a closed cavity when the sample target slot moves to the sample inlet; an exhaust filter is arranged in a vacuumizing gas path of the vacuum drying chamber and is used for filtering harmful impurities in the vacuum drying chamber; the sample inlet is communicated with the atmosphere through a valve.
Preferably, the sample inlet is communicated with the atmosphere through a valve and a gas inlet filter.
Preferably, at least two sample inlets are arranged on the cavity wall of the vacuum sample injection cavity, and each sample inlet is provided with the cabin door; at least two sample target grooves are arranged in the vacuum sample introduction cavity.
Preferably, the vacuum pumping system comprises a mechanical pump and a molecular pump, and the air inlet of the molecular pump is communicated with the vacuum sample feeding chamber through a pipeline; the exhaust port of the mechanical pump is communicated with the atmosphere through an exhaust filter, the air inlet of the mechanical pump is divided into two paths, the first path is communicated with the air outlet of the molecular pump through a valve, and the second path is communicated with the sample inlet of the vacuum sample injection cavity through a valve.
Preferably, the vacuum pumping system comprises a mechanical pump and a molecular pump, and the air inlet of the molecular pump is communicated with the vacuum sample feeding chamber through a pipeline; the exhaust port of the mechanical pump is communicated with the atmosphere through an exhaust filter, the air inlet of the mechanical pump is divided into two pipelines, the first pipeline is communicated with the air outlet of the molecular pump through a valve, and the second pipeline is communicated with the sample inlet of the vacuum sample injection chamber through a valve; the vacuum drying chamber is provided with two paths of vacuumizing pipelines, the first path of vacuumizing pipeline is communicated with an air inlet of the mechanical pump or the molecular pump through a valve, and the second path of vacuumizing pipeline is communicated with the atmosphere through a valve.
Preferably, the vacuum pumping system comprises a mechanical pump and a molecular pump, and the air inlet of the molecular pump is communicated with the vacuum sample feeding chamber through a pipeline; the exhaust port of the mechanical pump is communicated with the atmosphere through an exhaust filter, the air inlet of the mechanical pump is divided into two pipelines, the first pipeline is communicated with the air outlet of the molecular pump through a valve, and the second pipeline is communicated with the sample inlet of the vacuum sample injection chamber through a valve; the vacuum drying chamber is provided with two paths of vacuumizing pipelines, the first path of vacuumizing pipeline is communicated with an air inlet of the mechanical pump or the molecular pump through a valve, and the second path of vacuumizing pipeline is communicated with the atmosphere through the valve and the air inlet filter.
Preferably, the transition chamber and the vacuum drying chamber are the same chamber.
Preferably, the moving part is a remote control rail car, the remote control rail car is arranged on the track in the vacuum sample introduction chamber, and the remote control rail car is provided with a lifting mechanism for lifting/lowering the sample target slot.
The utility model discloses the advantage lies in need not to wait for the sample target dry, directly puts into the microbial mass spectrometer, and the mass spectrometer is accomplished by oneself and is done futilely to the sample target and carry out the mass spectrometry and detect. Greatly quickening the drying speed and ensuring the stability of the sample in the drying process. Because the exhaust filter is arranged, the biological safety problem in the drying process is avoided. Meanwhile, the whole drying process and the inlet and outlet sample targets are integrated together, so that the working steps of mass spectrum detection are greatly shortened, manual operation is not needed from the drying process to the detection process, errors possibly caused by manual participation are avoided, and the equipment operation efficiency is higher.
Drawings
FIG. 1 is a schematic diagram of the structure of a sample target processing system as described in example 1.
FIG. 2 is a flow chart of the vacuum drying of the sample target of example 1.
FIG. 3 is a schematic diagram of the structure of the sample target processing system described in example 2.
FIG. 4 is a flow chart of the vacuum drying of the sample target of example 2.
FIG. 5 is a schematic diagram of the structure of the sample target processing system described in example 3.
Detailed Description
Example 1:
as shown in fig. 1 and 2, the sample target processing system of the matrix assisted laser desorption ionization time-of-flight mass spectrometer of the present invention comprises a vacuum drying chamber 1 and a vacuum sample introduction chamber 2; the vacuum drying chamber 1 is used for drying a sample to be detected on a sample target, a moving part used for bearing a sample target groove 3 is arranged in the vacuum sample introduction chamber 2, and the sample target groove 3 is used for bearing the sample target; the moving part selects and preferably selects a remote control rail car 4, the remote control rail car 4 is arranged on a rail 5 in the vacuum sampling cavity 2, and the remote control rail car 4 is provided with a lifting mechanism for lifting/lowering the sample target slot 3; a transition chamber 6 is arranged at the sample inlet of the vacuum sample introduction chamber 2; the formation process of the transition chamber 6 is as follows: when the sample target slot 3 moves to the sample inlet and is lifted by the lifting/lowering mechanism to be close to the sample inlet, the sample target slot 3, the sample inlet and the door 7 are enclosed to form a closed cavity.
During the use, the sample target that the application of sample was accomplished not dry is put into vacuum drying chamber 1 and is carried out vacuum drying, treats the drying completion back, puts into the sample target groove 3 in vacuum sampling chamber 2 with the sample target after the drying, and sample target groove 3 removes in vacuum sampling chamber 2 under the drive of remote control railcar 4, realizes the detection of getting a design.
The vacuum-pumping system of the present embodiment includes a mechanical pump 8 and a molecular pump 9; an air inlet of a molecular pump 9 is communicated with a vacuum sample injection cavity 2, an air path between a mechanical pump 8 and the molecular pump 9 is provided with a valve 1.1, an air path between a transition cavity 6 and an air inlet of the mechanical pump 8 is provided with a valve 1.3, an air path between the vacuum drying cavity 1 and an air inlet of the mechanical pump 8 is provided with a valve 1.4, and the vacuum drying cavity 1 is vacuumized through the mechanical pump 8. A valve 1.5 is arranged between the vacuum drying chamber 1 and the atmosphere; a hatch door state switch 10 is arranged near the sample inlet of the vacuum sample introduction chamber 2 and is used for sensing the state of the hatch door 7 to be effectively closed or opened so as to adjust the opening and closing of the valve 1.2 and the valve 1.3 and avoid the situation that the vacuum sample introduction chamber 2 loses the vacuum degree because the hatch door 7 is not effectively closed and the valve 1.2 is opened. In order to prevent impurities in the atmospheric environment from entering the transition chamber 6 or the vacuum drying chamber 1, the air inlet filters 11 are arranged at the air inlets of the vacuum sampling chamber 2 and the vacuum drying chamber 1, and the air inlet filters 11 have a filtering and silencing effect, so that the phenomenon that after the valve 1.2 or the valve 1.5 is opened, gas rapidly rushes into the vacuum drying chamber 1 or the transition chamber 6 to generate noise pollution can be avoided.
The sample target processing system of this example has the following workflow:
firstly, as shown in fig. 2, when in use, the sample target after sample application is placed in the vacuum drying chamber 1, the valve 1.5 is closed, the valve 1.4 is opened, the vacuum drying chamber 1 is pumped to a vacuum state under the continuous operation of the mechanical pump 8 and is maintained for a preset time T, the internal moisture of the sample on the sample target is diffused to the surface through pressure difference or concentration difference in the vacuum environment, water molecules obtain enough kinetic energy on the surface of the sample, and the water molecules escape into low-pressure air after overcoming the intermolecular attraction force and are pumped away by the mechanical pump 8. The sample drying efficiency of the vacuum drying chamber 1 is far higher than that of natural drying, the stability of the sample in the vacuum drying process is far higher than that of a heating drying mode, and the drying process is realized in a closed space; an exhaust filter 12 is arranged at the exhaust port of the mechanical pump 8, so that harmful substances are prevented from being discharged into the atmosphere to pollute the environment.
In the process of vacuum drying of the sample target, the sample introduction system works normally, and the vacuum drying of the sample target has no influence on the sample introduction system.
Example 2:
as shown in fig. 3 and 4, the sample target processing system of the matrix assisted laser desorption ionization time-of-flight mass spectrometer of the present invention comprises a vacuum sample introduction chamber 1a, wherein a moving part for bearing a sample target groove 2a is arranged in the vacuum sample introduction chamber 1a, and the sample target groove 2a is used for bearing a sample target; the moving part selects and prefers to be a remote control rail car 3a, the remote control rail car 3a is arranged on a rail 4a in the vacuum sampling cavity 1a, and the remote control rail car 3a is provided with a lifting mechanism for lifting/lowering the sample target slot 2 a; a transition chamber 5a which is also used as a vacuum drying chamber is arranged at the sample inlet of the vacuum sample feeding chamber 1a, that is, the vacuum drying chamber and the transition chamber in the embodiment are the same chamber. In other embodiments of the present invention, the moving portion may have other structures as long as it can perform the moving and lifting functions of the sample target well 2 a.
The transition chamber 5a is formed by the following process: when the sample target slot 2a moves to the sample inlet and is lifted by the lifting/lowering mechanism to be close to the sample inlet, the sample target slot 2a, the sample inlet and the hatch 6a are enclosed to form a closed cavity.
The vacuum pumping system of the present embodiment includes a mechanical pump 7a and a molecular pump 8 a; a valve 2.1 is arranged on an air path between the mechanical pump 7a and the molecular pump 8a, and an air inlet of the molecular pump 8a is communicated with the vacuum sample injection chamber 1 a; the air passage between the transition chamber 5a and the air inlet of the mechanical pump 7a is provided with a valve 2.3, and the air outlet of the mechanical pump 7a is provided with an exhaust filter 11a, so that harmful substances are prevented from being discharged into the atmosphere to pollute the environment. In order to prevent impurities in the atmospheric environment from entering the transition chamber 5a and the vacuum sampling chamber 1a, an air inlet filter 9a is arranged at an air inlet of the vacuum sampling chamber 1a, and the air inlet filter 9a has a filtering and silencing effect, so that the phenomenon that after the valve 2.2 is opened, gas rapidly rushes into the transition chamber 5a and the vacuum sampling chamber 1a to generate noise-producing pollution can be avoided.
A hatch door state switch 10a is arranged near the sample inlet of the vacuum sample introduction chamber 1a and is used for sensing the state of the hatch door 6a to be effectively closed or opened so as to adjust the opening and closing of the valve 2.2 and the valve 2.3 and avoid the situation that the hatch door 6a is not effectively closed and opens the valve 2.2 to cause the vacuum sample introduction chamber 1a to lose the vacuum degree.
In use, the sample target which is not dried after being loaded is placed in the sample target groove 2a at the transition chamber 5a, the hatch door 6a is closed, and the valve 2 is closed1 and 2.2, opening the valve 2.3, starting the vacuum drying mode at the moment, and vacuumizing the transition chamber 5a by the mechanical pump 7a through the gas circuit of the valve 2.3 to create a vacuum drying environment for the sample target; the vacuum degree in the transition chamber 5a is maintained at 10- 2And mbar magnitude order, after the vacuum drying mode lasts for a preset time T, the sample on the sample target is rapidly dried, and in the process, the exhaust filter 11a at the exhaust port of the mechanical pump 7a continuously filters the gas extracted by vacuum drying, so that the biosafety risk is more effectively controlled. And after the preset time T, completing vacuum drying, closing the valve 2.3, opening the valve 2.1, and moving the sample target to a mass spectrum sampling position through the remote control rail car 3a, so that mass spectrum sampling can be performed.
The transition chamber 5a has reached a vacuum degree of 10 before the transition chamber 5a is integrated with the vacuum sampling chamber 1a- 2mbar order of magnitude, and transition cavity 5a volume is very little, and the pumping speed of the mode that mechanical pump 7a concatenates molecular pump 8a is very big, can advance the vacuum rapidly and advance the inside vacuum degree of sample cavity 1a and resume, so advance the inside vacuum degree influence of sample cavity 1a very little to the realization sample is sampled and is detected (or called and is called the detection of drawing a design).
When the sample target is discharged, the sample target moves to the position of the hatch 6a, at this time, the valve 2.1 and the valve 2.3 are closed, the valve 2.2 is opened, air is put into the transition chamber 5a, the air pressure in the transition chamber 5a is the same as the external ambient air pressure, and at this time, the hatch 6a can be opened to replace the sample target.
Example 3:
as shown in fig. 5, the present embodiment is different from embodiment 2 only in that two sample inlets are disposed on the sidewall of the vacuum sample chamber 1b, the two sample inlets are respectively provided with two hatches 2.1b and 2.2 b, correspondingly, two sample target slots 3.1b and 3.2b are disposed in the vacuum sample chamber 1b, and the moving portion can independently drive any one of the sample target slots to move. The air inlet of the mechanical pump 4b is divided into two air paths, and one air path is communicated with the molecular pump 6b through a valve 3.1; the other path is communicated with one sample inlet through a valve 3.2 and is communicated with the other sample inlet through a valve 3.3; the two transition chambers 5.1b, 5.2b are connected to the atmosphere via a valve 3.4, a valve 3.5 and an inlet filter 7b, respectively, and the mechanical pump 4b has its exhaust connected to the atmosphere via an exhaust filter 8 b.
In order to avoid the influence of time consumption on the detection efficiency of the mass spectrometer when the sample target is dried in the transition chamber (the vacuum drying chamber and the transition chamber are the same chamber), when the sample target slot is used, the sample target slot 3.1b and the sample target slot 3.2b are respectively and correspondingly arranged at the two sample inlets, so that two transition chambers 5.1b and 5.2b can be formed.
During detection, sample targets after point sample completion can be placed in each transition cavity 5.1b and 5.2b in sequence for vacuum drying, and according to different drying completion time, the moving parts are fully utilized to drive the corresponding sample target grooves 3.1b and 3.2b to move to mass spectrum sampling positions, so that mass spectrometer uninterrupted mass spectrum sampling is realized. Meanwhile, the rest transition chambers 5.1b and 5.2b reach a low vacuum state in the sampling process, so that the time for waiting for vacuumizing in the transition chambers 5.1b and 5.2b is saved, and the detection efficiency of the mass spectrometer is greatly improved.
Claims (8)
1. A sample target processing system of a matrix-assisted laser desorption ionization time-of-flight mass spectrometer is characterized in that: comprises a vacuum pumping system, a vacuum sample introduction chamber provided with a cabin door, and a vacuum drying chamber used for drying a sample target to be tested; a moving part for bearing a sample target groove is arranged in the vacuum sample introduction chamber, and the sample target groove is used for bearing a sample target to be detected; a transition chamber is arranged at a sample inlet of the vacuum sample introduction chamber and is used for enabling a sample target slot, the sample inlet and the cabin door to enclose to form a closed cavity when the sample target slot moves to the sample inlet; an exhaust filter is arranged in a vacuumizing gas path of the vacuum drying chamber and is used for filtering harmful impurities in the vacuum drying chamber; the sample inlet is communicated with the atmosphere through a valve.
2. The matrix assisted laser desorption ionization time-of-flight mass spectrometer sample target processing system of claim 1, wherein: the sample inlet is communicated with the atmosphere through a valve and an air inlet filter.
3. The matrix assisted laser desorption ionization time-of-flight mass spectrometer sample target processing system of claim 2, wherein: the cavity wall of the vacuum sample injection cavity is provided with at least two sample injection ports, and each sample injection port is provided with the cabin door; at least two sample target grooves are arranged in the vacuum sample introduction cavity.
4. The matrix assisted laser desorption ionization time-of-flight mass spectrometer sample target processing system of any one of claims 1 to 3, wherein: the vacuum pumping system comprises a mechanical pump and a molecular pump, and an air inlet of the molecular pump is communicated with the vacuum sample injection chamber through a pipeline; the exhaust port of the mechanical pump is communicated with the atmosphere through an exhaust filter, the air inlet of the mechanical pump is divided into two paths, the first path is communicated with the air outlet of the molecular pump through a valve, and the second path is communicated with the sample inlet of the vacuum sample injection cavity through a valve.
5. The matrix assisted laser desorption ionization time-of-flight mass spectrometer sample target processing system of claim 1, wherein: the vacuum pumping system comprises a mechanical pump and a molecular pump, and an air inlet of the molecular pump is communicated with the vacuum sample injection chamber through a pipeline; the exhaust port of the mechanical pump is communicated with the atmosphere through an exhaust filter, the air inlet of the mechanical pump is divided into two pipelines, the first pipeline is communicated with the air outlet of the molecular pump through a valve, and the second pipeline is communicated with the sample inlet of the vacuum sample injection chamber through a valve; the vacuum drying chamber is provided with two paths of vacuumizing pipelines, the first path of vacuumizing pipeline is communicated with an air inlet of the mechanical pump or the molecular pump through a valve, and the second path of vacuumizing pipeline is communicated with the atmosphere through a valve.
6. The matrix assisted laser desorption ionization time-of-flight mass spectrometer sample target processing system of claim 2, wherein: the vacuum pumping system comprises a mechanical pump and a molecular pump, and an air inlet of the molecular pump is communicated with the vacuum sample injection chamber through a pipeline; the exhaust port of the mechanical pump is communicated with the atmosphere through an exhaust filter, the air inlet of the mechanical pump is divided into two pipelines, the first pipeline is communicated with the air outlet of the molecular pump through a valve, and the second pipeline is communicated with the sample inlet of the vacuum sample injection chamber through a valve; the vacuum drying chamber is provided with two paths of vacuumizing pipelines, the first path of vacuumizing pipeline is communicated with an air inlet of the mechanical pump or the molecular pump through a valve, and the second path of vacuumizing pipeline is communicated with the atmosphere through the valve and the air inlet filter.
7. The matrix assisted laser desorption ionization time-of-flight mass spectrometer sample target processing system of any one of claims 1, 3 or 5, wherein: the transition chamber and the vacuum drying chamber are the same chamber.
8. The matrix assisted laser desorption ionization time-of-flight mass spectrometer sample target processing system of claim 1 or 3, wherein: the moving part is a remote control rail car, the remote control rail car is arranged on the track in the vacuum sample introduction cavity, and a lifting mechanism used for lifting/descending the sample target slot is arranged on the remote control rail car.
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CN113745091A (en) * | 2021-09-15 | 2021-12-03 | 深圳泰莱生物科技有限公司 | Control system and method of mass spectrum device |
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CN113745091A (en) * | 2021-09-15 | 2021-12-03 | 深圳泰莱生物科技有限公司 | Control system and method of mass spectrum device |
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