CN214142380U - Controllable oxygen accuse temperature atmospheric pressure pressurization cell culture device - Google Patents
Controllable oxygen accuse temperature atmospheric pressure pressurization cell culture device Download PDFInfo
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- CN214142380U CN214142380U CN202022917890.6U CN202022917890U CN214142380U CN 214142380 U CN214142380 U CN 214142380U CN 202022917890 U CN202022917890 U CN 202022917890U CN 214142380 U CN214142380 U CN 214142380U
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title claims abstract description 38
- 239000001301 oxygen Substances 0.000 title claims abstract description 38
- 229910052760 oxygen Inorganic materials 0.000 title claims abstract description 38
- 238000004113 cell culture Methods 0.000 title claims abstract description 32
- 238000010438 heat treatment Methods 0.000 claims abstract description 21
- 230000033228 biological regulation Effects 0.000 claims abstract description 8
- 238000007789 sealing Methods 0.000 claims description 6
- 238000001514 detection method Methods 0.000 abstract description 6
- 239000007789 gas Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000010367 cloning Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000013401 experimental design Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000012606 in vitro cell culture Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000010365 information processing Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
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Abstract
The utility model discloses a belongs to the technical field of cell culture, in particular to a controllable oxygen temperature control air pressure pressurization cell culture device, which comprises an incubator, a chamber door, a detection component, a regulation and control component and a micro air pump, wherein the detection component comprises a temperature and humidity sensor, an air pressure sensor and an oxygen detector, the regulation and control component comprises a control panel, a heating coil, an air humidifier and an air booster pump, the detected data information is transmitted to the control panel in real time through the temperature and humidity sensor, the air pressure sensor and the oxygen detector, the temperature and humidity, the air pressure and the oxygen content information in the culture chamber can be monitored in real time, the control panel can receive the data information conveniently, the heating coil, the air humidifier and the air booster pump are controlled to work according to a preset value, the culture condition in the culture chamber is changed, and the intelligentization and the automation are strong, meanwhile, the air in the culture cavity is pumped by the matching of the miniature air pump and the connecting pipe, so that the air circulation in the culture cavity is increased.
Description
Technical Field
The utility model relates to a cell culture technical field specifically is a controllable oxygen accuse temperature atmospheric pressure pressurization cell culture device.
Background
Cell culture (cellcell) refers to a method of simulating in vivo environment (sterile, suitable temperature, humidity and certain nutritional conditions) in vitro to allow cells to survive, grow, proliferate and maintain their primary structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technology can be used for culturing a single cell into simple single cells or cells with few differentiation in a large scale, which is an indispensable link of the cloning technology, and the cell culture is the cloning of the cell. Cell culture technology is an important and common technology in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of the cells and the like of the cells can be researched by the cell culture technology.
Oxygen, temperature, pressure intervention requirements for cell culture: the oxygen concentration can be controlled at will within the range of experimental design and monitored continuously; the pressure in the culture tank can be controlled within the range of experimental design, and the temperature of the in vitro cell culture environment is consistent with that of a conventional cell culture box for a laboratory. Otherwise, the experimental results are not scientific, so it is urgently needed to provide a controllable oxygen temperature-controlled air pressure-pressurized cell culture device.
SUMMERY OF THE UTILITY MODEL
This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and in the abstract of the specification and the title of the application to avoid obscuring the purpose of this section, the abstract of the specification and the title of the application, and such simplifications or omissions are not intended to limit the scope of the invention.
In view of the above and/or problems with the prior art culture devices, the present invention has been made.
Therefore, the utility model aims at providing a controllable oxygen accuse temperature atmospheric pressure pressurization cell culture device, the problem of cell culture's requirement under can't satisfying oxygen, temperature, the pressure intervention of equipment now.
For solving the technical problem, according to the utility model discloses an aspect, the utility model provides a following technical scheme:
a controllable oxygen temperature-controlled air pressure pressurized cell culture device, comprising: the incubator comprises an incubator body, a chamber door, a detection assembly, a regulation assembly and a miniature air pump, wherein the outer side wall of the incubator body is connected with the chamber door, the incubator body is divided into a left chamber culture chamber and a right chamber culture chamber and a connecting chamber by a partition plate, the detection assembly comprises a temperature and humidity sensor, an air pressure sensor and an oxygen detector, the temperature and humidity sensor is installed at the top of the right side of the inner side wall of the culture chamber, the air pressure sensor is arranged at the right side of the inner side wall of the culture chamber, the air pressure sensor is positioned below the temperature and humidity sensor, the oxygen detector is installed at the central position of the top of the inner side wall of the culture chamber, the regulation assembly comprises a control panel, a heating coil, an air humidifier and an air booster pump, the control panel is embedded and installed on the front side wall of the chamber door, the bottom of the inner side wall of the culture chamber is provided with a plurality of heating coils, and the air humidifier is arranged at the top of the left side wall of the culture chamber, the air booster pump is arranged at the bottom of the inner side wall of the connecting cavity, and the miniature air pump is arranged at the top of the outer side wall of the incubator.
As the utility model discloses a controllable oxygen accuse temperature atmospheric pressure pressurization cell culture device an preferred scheme, wherein: the inner side wall of the box door is bonded with a sealing washer corresponding to the joint of the incubator, and the central position of the box door is provided with an observation window.
As the utility model discloses a controllable oxygen accuse temperature atmospheric pressure pressurization cell culture device an preferred scheme, wherein: the equal rigid coupling in cultivation intracavity lateral wall left and right sides has the connecting plate, two the connecting plate symmetry sets up, and two the connecting plate is located heating coil with between the temperature and humidity sensor, two sliding connection has between the connecting plate and places the board.
As the utility model discloses a controllable oxygen accuse temperature atmospheric pressure pressurization cell culture device an preferred scheme, wherein: the input port of the miniature air pump is communicated with the incubator through a connecting pipe.
As the utility model discloses a controllable oxygen accuse temperature atmospheric pressure pressurization cell culture device an preferred scheme, wherein: a plurality of the heating coil is followed the setting is arranged to the linear equidistance from a left side to the right side of culture chamber inside wall.
Compared with the prior art: through temperature and humidity sensor, baroceptor and oxygen detector and control panel electric connection, data information real-time transmission to control panel that will detect, can real-time supervision cultivate the inside humiture of chamber, atmospheric pressure and oxygen content information, make things convenient for control panel to receive data information, control heating coil according to the default, air humidifier and air booster pump work, it changes to cultivateing the inside condition of cultivateing of intracavity, it is intelligent and automatic stronger, and simultaneously, extract the gas of cultivateing the intracavity through miniature air pump and connecting pipe cooperation, increase and cultivate the inside gas circulation nature of chamber, and seal ring's setting can increase the connection leakproofness between chamber door and the incubator, and then increase cultivation effect and success rate.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the present invention will be described in detail with reference to the accompanying drawings and detailed embodiments, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive labor. Wherein:
FIG. 1 is a schematic view of the overall structure of the present invention;
fig. 2 is a schematic view of the front view structure of the present invention.
In the figure: 100 incubator, 110 incubation chamber, 111 slide plate, 120 connection chamber, 130 placing plate, 200 box door, 210 sealing gasket, 220 observation window, 300 detection component, 310 temperature and humidity sensor, 320 air pressure sensor, 330 oxygen detector, 400 regulation component, 410 control panel, 420 heating coil, 430 air humidifier, 440 air booster pump and 500 micro air pump.
Detailed Description
In order to make the above objects, features and advantages of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be implemented in other ways than those specifically described herein, and one skilled in the art may similarly generalize the present invention without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
Next, the present invention will be described in detail with reference to the schematic drawings, and in the detailed description of the embodiments of the present invention, for convenience of explanation, the sectional view showing the device structure will not be enlarged partially according to the general scale, and the schematic drawings are only examples, and should not limit the scope of the present invention. In addition, the three-dimensional dimensions of length, width and depth should be included in the actual fabrication.
In order to make the objects, technical solutions and advantages of the present invention clearer, embodiments of the present invention will be described in further detail below with reference to the accompanying drawings.
The utility model provides a controllable oxygen accuse temperature atmospheric pressure pressurization cell culture device, through temperature and humidity sensor, baroceptor and oxygen detector and control panel electric connection, data information with detecting transmits to control panel in real time, can real-time supervision cultivate the intracavity humiture, atmospheric pressure and oxygen content information, make things convenient for control panel to receive data information, control heating coil, air humidifier and air booster pump work according to the default, change the cultivation condition of cultivating the intracavity, it is intelligent and automatic stronger, please refer to figure 1-2, include, incubator 100, chamber door 200, detection component 300, regulation and control component 400 and miniature air pump 500;
with reference to fig. 1-2, the incubator 100 is divided into a left chamber culture chamber 110 and a right chamber culture chamber 120 by a partition board, the left side and the right side of the inner side wall of the culture chamber 110 are respectively screwed with a connecting plate 111, the two connecting plates 111 are symmetrically arranged, the two connecting plates 111 are located between the heating coil 420 and the temperature and humidity sensor 310, a placing plate 130 is slidably connected between the two connecting plates 111, and the connecting plates 111 are slidably connected with the placing plate 130, so that the placing and withdrawing operations of the culture dish are facilitated;
referring to fig. 1-2, the outer side wall of the incubator 100 is connected to the chamber door 200, a sealing gasket 210 is adhered to the inner side wall of the chamber door 200 corresponding to the connection with the incubator 100, the sealing gasket 210 is used to increase the connection sealing performance between the chamber door 200 and the incubator 100 and increase the incubation effect, and an observation window 220 is disposed in the center of the chamber door 200;
referring to fig. 1, the detecting assembly 300 includes a temperature and humidity sensor 310, an air pressure sensor 320 and an oxygen detector 330, specifically, the temperature and humidity sensor 310 is screwed to the top of the right side of the inner sidewall of the cultivation chamber 110, the temperature and humidity sensor 310 is used for monitoring temperature and humidity information inside the cultivation chamber 110 in real time, the air pressure sensor 320 is embedded in the right side of the inner sidewall of the cultivation chamber 110, the air pressure sensor 320 is used for monitoring air pressure data information inside the cultivation chamber 110 in real time, the air pressure sensor 320 is located below the temperature and humidity sensor 310, the oxygen detector 330 is clamped at the central position of the top of the inner sidewall of the cultivation chamber 110, the oxygen detector 330 is used for monitoring oxygen concentration content inside the cultivation chamber 110 in real time, the temperature and humidity sensor 310, the air pressure sensor 320 and the oxygen detector 330 are electrically connected to the control panel 410, detected data information is transmitted to the control panel 410 in real time, and the control panel 410 is convenient to control the heating coil 420, the oxygen detector 330, The air humidifier 430 and the air booster pump 440 work to change the culture conditions inside the culture chamber 110;
referring to fig. 1-2, the control assembly 400 includes a control panel 410, a heating coil 420, an air humidifier 430 and an air booster pump 440, specifically, the control panel 410 is embedded in the front side wall of the door 200, the control panel 410 is used as the operation core and the control core of the apparatus, and is the final execution unit for information processing and program operation, the bottom of the inner side wall of the culture chamber 110 is connected with five heating coils 420 in a clamping manner, the five heating coils 420 are linearly and equidistantly arranged from left to right along the inner side wall of the culture chamber 110, the heating coils 420 are used for performing auxiliary heating on the inside of the culture chamber 110, so as to control the temperature of the cell culture environment in cooperation with the control panel 410, the air humidifier 430 is screwed on the top of the left side wall of the culture chamber 110, the air humidifier 430 is used for performing auxiliary humidification on the inside of the culture chamber 110, and the auxiliary humidification on the inside of the culture chamber 110 to the optimal humidity for culture, the air booster pump 440 is connected to the bottom of the inner side wall of the connecting chamber 120 through a fastening screw thread, and the air booster pump 440 is used for performing auxiliary pressurization operation on the inside of the culture chamber 110 in real time;
referring to fig. 1-2, the micro air pump 500 is screwed to the top of the outer sidewall of the incubator 100 by a fastening screw, an input port of the micro air pump 500 is connected to the incubator 100 by a connecting tube, and the micro air pump 610 is matched with the connecting tube to pump the air in the culture chamber 110, thereby increasing the air circulation inside the culture chamber 110.
The working principle is as follows: the utility model discloses a when using, through temperature and humidity sensor 310, baroceptor 320 and oxygen detector 330 and control panel 410 electric connection, data information real-time transmission to control panel 410 that will detect, can real-time supervision cultivate the inside humiture of chamber 110, atmospheric pressure and oxygen content information, make things convenient for control panel 410 to receive data information, control heating coil 420 according to the default, air humidifier 430 and air booster pump 440 work, it changes to cultivateing the inside condition of cultivateing chamber 110, it is intelligent and automatic stronger, and simultaneously, extract the gas of cultivateing in the chamber 110 through miniature air pump 610 and the pipe connection cooperation, increase the inside gas circulation nature of cultivateing chamber 110, and seal ring 210's setting can increase the connection leakproofness between chamber door 200 and incubator 100, and then increase cultivation effect and success rate.
While the invention has been described above with reference to an embodiment, various modifications may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention. In particular, as long as there is no structural conflict, the various features of the disclosed embodiments of the present invention can be used in any combination with each other, and the non-exhaustive description of these combinations in this specification is merely for the sake of brevity and resource conservation. Therefore, it is intended that the invention not be limited to the particular embodiments disclosed, but that the invention will include all embodiments falling within the scope of the appended claims.
Claims (5)
1. A controllable oxygen accuse temperature atmospheric pressure pressurization cell culture device which characterized in that includes: incubator (100), chamber door (200), determine module (300), regulation and control subassembly (400) and miniature air pump (500), incubator (100) lateral wall is connected chamber door (200), incubator (100) divide into about two cavities through the baffle and cultivate chamber (110) and connect chamber (120), determine module (300) includes temperature and humidity sensor (310), baroceptor (320) and oxygen detector (330), temperature and humidity sensor (310) install in cultivate chamber (110) inside wall right side top, baroceptor (320) are located cultivate chamber (110) inside wall right side, baroceptor (320) are located temperature and humidity sensor (310) below, oxygen detector (330) install in cultivate chamber (110) inside wall top central point and put, regulation and control subassembly (400) include control panel (410), Heating coil (420), air humidifier (430) and air booster pump (440), control panel (410) embedding install in lateral wall before chamber door (200), cultivate chamber (110) inside wall bottom and set up a plurality ofly heating coil (420), air humidifier (430) are located cultivate chamber (110) inside wall left side top, air booster pump (440) are located connect chamber (120) inside wall bottom, miniature air pump (500) install in incubator (100) lateral wall top.
2. The cell culture apparatus according to claim 1, wherein a sealing gasket (210) is bonded to the inner sidewall of the door (200) corresponding to the connection with the incubator (100), and a viewing window (220) is opened at the center of the door (200).
3. The cell culture device according to claim 1, wherein the left side and the right side of the inner side wall of the culture cavity (110) are fixedly connected with connecting plates (111), the two connecting plates (111) are symmetrically arranged, the two connecting plates (111) are located between the heating coil (420) and the temperature and humidity sensor (310), and a placing plate (130) is slidably connected between the two connecting plates (111).
4. The controllable oxygen temperature-controlled air pressure-pressurized cell culture device according to claim 1, wherein the input port of the micro air pump (500) is connected with the incubator (100) through a connecting pipe.
5. The controllable oxygen-controlled air pressure-controlled cell culture device according to claim 1, wherein the heating coils (420) are linearly arranged along the inner side wall of the culture chamber (110) from left to right at equal intervals.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022917890.6U CN214142380U (en) | 2020-12-08 | 2020-12-08 | Controllable oxygen accuse temperature atmospheric pressure pressurization cell culture device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022917890.6U CN214142380U (en) | 2020-12-08 | 2020-12-08 | Controllable oxygen accuse temperature atmospheric pressure pressurization cell culture device |
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| Publication Number | Publication Date |
|---|---|
| CN214142380U true CN214142380U (en) | 2021-09-07 |
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| CN202022917890.6U Active CN214142380U (en) | 2020-12-08 | 2020-12-08 | Controllable oxygen accuse temperature atmospheric pressure pressurization cell culture device |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Shaanxi Paidijian Biotechnology Co.,Ltd. Assignor: XI'AN PEIHUA University Contract record no.: X2024980003889 Denomination of utility model: A controllable oxygen, temperature, pressure, and pressure controlled cell culture device Granted publication date: 20210907 License type: Common License Record date: 20240402 |
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| EE01 | Entry into force of recordation of patent licensing contract |