CN215209345U - Microbial fermentation detection device - Google Patents
Microbial fermentation detection device Download PDFInfo
- Publication number
- CN215209345U CN215209345U CN202120542114.4U CN202120542114U CN215209345U CN 215209345 U CN215209345 U CN 215209345U CN 202120542114 U CN202120542114 U CN 202120542114U CN 215209345 U CN215209345 U CN 215209345U
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- fermentation
- detection device
- light
- light source
- pipe
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- 230000004151 fermentation Effects 0.000 title claims abstract description 84
- 238000000855 fermentation Methods 0.000 title claims abstract description 82
- 238000001514 detection method Methods 0.000 title claims abstract description 49
- 230000000813 microbial effect Effects 0.000 title claims abstract description 21
- 244000005700 microbiome Species 0.000 claims abstract description 9
- 230000005540 biological transmission Effects 0.000 claims description 19
- 239000011521 glass Substances 0.000 claims description 4
- 239000002274 desiccant Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 10
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 230000001580 bacterial effect Effects 0.000 abstract description 4
- 230000008859 change Effects 0.000 abstract description 4
- 238000009434 installation Methods 0.000 abstract description 3
- 241001052560 Thallis Species 0.000 abstract 1
- 230000003287 optical effect Effects 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 description 14
- 239000012535 impurity Substances 0.000 description 4
- 238000009655 industrial fermentation Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000004103 aerobic respiration Effects 0.000 description 2
- 230000004099 anaerobic respiration Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000007306 turnover Effects 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000023077 detection of light stimulus Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 230000007269 microbial metabolism Effects 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
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- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000004879 turbidimetry Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a microbial fermentation detection device includes fermentation cylinder and detection device, detection device includes the light source, the printing opacity pipe, photoelectric detector, casing and operating panel, the casing is installed on the fermentation cylinder lateral wall, the light source, printing opacity pipe and photoelectric detector install in the casing, operating panel installs on the housing face, wherein the installation of fermentation cylinder lateral wall is hugged closely to the light source, the printing opacity pipe is installed in the light source outside, and return bend and the inside intercommunication of fermentation cylinder are passed through at both ends about the printing opacity pipe, photoelectric detector installs on the casing in the printing opacity outside, the concentration according to the microorganism suspension is in certain extent and the fermentation of optical density direct ratio detection microorganism. The utility model discloses a mode of intercommunication keeps the fermented material to be in same environment, can realize carrying out real-time supervision to the microbial bacterial volume in the fermenting installation simultaneously, in time detects the change of thalli quantity, ensures fermentation production process safety and stability, and the operation is simpler, and the probability that the mistake takes place moreover is lower.
Description
Technical Field
The utility model belongs to the technical field of little biological detection's technique and specifically relates to a microbial fermentation detection device is related to.
Background
Microbial fermentation refers to a process of converting raw materials into products required by human beings through a specific metabolic pathway by using microorganisms under appropriate conditions. The level of microbial fermentation production depends mainly on the genetic characteristics of the strain itself and the culture conditions. Fermentation is a common chemical reaction, widely used in lT industries such as biology, food, chemistry, etc., and biomass (mainly referred to as microbial biomass) is constantly changed during fermentation, so that accurate monitoring of the biomass is required for fermentation research.
The industrial fermentation process has the following characteristics: industrial fermentation (a) relies on the vital activities of microorganisms, which are supported by the metabolic energy provided by biological oxidation, and thus industrial fermentation covers all modes of biological oxidation in microbial physiology: aerobic respiration, anaerobic respiration and fermentation. The fermentation raw material generally takes starch, molasses or other agricultural and sideline products as main materials, and the fermentation scale can reach dozens or even hundreds of tons. The industrial fermentation process is completed by the automatic regulation or stress mode of organism, the reaction is generally aimed at obtaining a specific substance, and the fermentation process is regulated to make the substance accumulate so as to obtain the product. And (IV) the seed production process and the fermentation process are divided into two parts and are not in one reactor. Because the industrial production process control belongs to the same mode and the control of the bacterial quantity is directly related to the subsequent fermentation conditions, the bacterial quantity of the seeds must be reasonably controlled. The initial growth of the microorganisms is generally in an exponential growth mode, real-time bacteria amount monitoring has great significance to fermentation, the bacteria amount is likely to be changed by times without timely detection, the requirement on subsequent nutrient substances becomes harsh, secondary metabolites are generated in large quantity, and meanwhile feedback inhibition on the bacteria can be generated to influence production.
The existing microbial fermentation detection usually adopts an external detection device for detection, fermentation liquor in the fermentation device needs to be extracted for detection in the detection process, but the fermentation device has a larger difference with the external environmental conditions in the extraction process, the microbial metabolism speed is high, the change of the environmental conditions can cause the change of a microbial sample, and a larger error occurs in the detection result; even if professional detection equipment is lacked on part of the fermentation devices, only an observation window is arranged for detecting the fermentation process, and the detection effect is poor, the precision is low, and the practicability is not high.
SUMMERY OF THE UTILITY MODEL
To the problem that exists among the current microbial fermentation detection, the utility model discloses a device that utilizes the turbidimetry to carry out microbial detection through with zymotic fluid circulation drainage in the fermentation cylinder.
The utility model provides a scheme that its technical problem adopted is: a microbial fermentation detection device comprises a fermentation tank and a detection device, wherein the detection device is arranged on the side wall of the fermentation tank and comprises a light source, a light transmitting tube, a photoelectric detector, a shell and an operation panel;
wherein the light source is hugged closely the installation of fermentation cylinder lateral wall, and the printing opacity pipe is installed in the light source outside, and both ends pass through return bend and the inside intercommunication of fermentation cylinder about the printing opacity pipe, and the photoelectric detector is installed in the printing opacity outside of managing, is located the medial surface of casing.
Furthermore, a micro pump is arranged on the elbow pipe at the lower end of the light transmission pipe and used for circular drainage of fermentation liquor.
Further, the return bend tip at printing opacity pipe both ends is provided with screw thread cutting ferrule and connects, is provided with two root length connecting pipes about being provided with on the fermentation cylinder, and the tip of connecting pipe sets up the external screw thread, installs the printing opacity pipe on the fermentation cylinder through screw thread cutting ferrule and connecting pipe.
Furthermore, a filter screen is arranged at the connecting part of the lower end of the light transmission pipe and the fermentation tank, so that the influence of impurities in the fermentation liquor is avoided.
Furthermore, the light-transmitting tube adopts a high-light-transmitting glass tube, and bent tubes are arranged at two ends of the glass tube to form a U-shaped tube structure.
Further, a desiccant is placed in the housing.
The beneficial effects of the utility model
The utility model discloses a mode that printing opacity pipe and fermentation cylinder directly communicate circulates fermented material drainage to printing opacity pipe, keeps fermented material in same environment, recycles the fermented material detection of light source and detection device in to the printing opacity pipe to comparatively accurate detecting fermentation microorganism, and through the utility model discloses a detection device can realize carrying out real-time supervision to the microbial bacterial volume in the fermentation device, in time detects the change of thallus quantity, ensures fermentation production process safety and stability.
The micropump is arranged at the lower end of the light transmission pipe, the pump body is utilized to realize active circulation of the fermented substances, so that the fermented substances are prevented from being precipitated or entering the light transmission pipe unevenly, the active circulation ensures that the fermented substances in the light transmission pipe are kept consistent with the fermented substances in the fermentation tank to the maximum extent, and the accuracy of the detection structure is improved; meanwhile, a filter screen is arranged at the initial position of the fermentation material circulation, so that the influence of large impurities in the fermentation device on the detection is avoided.
The light-transmitting pipe and the fermentation tank are installed in a closed mode, microbial fermentation detection can be performed in various processes of aerobic respiration, anaerobic respiration, fermentation and the like according to the type of the fermentation device, and the application range of the detection device is widened.
Drawings
Fig. 1 is a cross-sectional view of the present invention.
FIG. 2 is a partial cross-sectional view of a detection device.
FIG. 3 is a cross-sectional view of another embodiment of the test device.
FIG. 4 is a cross-sectional view of yet another embodiment of a detection device.
Reference numbers in the figures: the fermentation tank 1, the detection device 2, the light source 21, the light transmission tube 22, the photoelectric detector 23, the shell 24, the operation panel 25, the threaded sleeve connector 221, the connecting tube 222, the micropump 3 and the filter screen 4.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in detail with reference to the accompanying drawings.
Example 1: in order to improve the efficiency that microbial fermentation detected, realize the purpose of incessant detection simultaneously, the utility model provides a microbial fermentation detection device as shown in figure 1 includes fermentation cylinder 1 and detection device 2, at the outside wall mounting detection device of fermentation cylinder 1 to through with detection device and the inside intercommunication of fermentation cylinder 1, realize microbial fermentation's real-time detection.
The detection device 2 is shown in fig. 2 and comprises a light source 21, a light transmitting tube 22, a photoelectric detector 23, a casing 24 and an operation panel 25, wherein the casing 24 is welded on the fermentation tank 1, one side of the casing 24 is provided with a turnover door, the opening and closing of the casing 24 are realized by opening and closing the turnover door, the light source 21, the light transmitting tube 22 and the photoelectric detector 23 are sequentially installed in the casing 24, the light source 21 is installed on the side wall of the fermentation tank 1, the light transmitting tube 22 is installed on the outer side of the light source 21, bent tube joints are installed at the upper end and the lower end of the light transmitting tube 22 and form a U-shaped structure with the light transmitting tube 22, the light transmitting tube 22 is communicated with the fermentation tank 1, and therefore fermented substances in the fermentation tank 1 can circulate in the light transmitting tube 22. The photoelectric detector 23 is installed outside the light transmission tube 22 and used for receiving and detecting light rays emitted by the light source 21 and filtered by fermentation materials in the light transmission tube 22, and the photoelectric detector 23 can be installed in the shell in a suspended mode or directly installed on the side wall of the shell according to actual conditions.
The operation panel 25 is installed on the outer side of the casing 24, and the operation panel 25 includes a display portion and an operation portion, the operation portion is used for controlling the specific behavior setting of the detection device 2, and the display portion is used for displaying the detection result in real time, so that the detection data can be conveniently and visually checked. And the touch display panel can be selected for use to realize control and display simultaneously for convenience and rapidness in operation.
Example 2: since a part of the fermented material is relatively viscous and the size of the light-transmitting tube 22 is relatively small, the viscous fermented material is not uniformly diffused in the light-transmitting tube 22. In order to solve the problem, on the basis of the embodiment 1, as shown in fig. 2, a micro pump 3 is added at the bent pipe at the lower end of the light transmission pipe 22, the fermented substance is pumped out from the lower end by the micro pump 3 and circularly flows along the light transmission pipe 22, so that the fermented substance in the light transmission pipe 22 and the substances in the fermentation tank are continuously circulated, the substances in the light transmission pipe and the substances in the fermentation tank are kept constant, and the reliable and real detection result is ensured.
Example 3: as shown in FIG. 3, the light-transmitting pipe 22 is installed in the fermentation tank by providing a screw clamp connector 221 at the bent pipe at both ends of the light-transmitting pipe 22, installing a connection pipe 222 having an external screw thread at the end part thereof in the fermentation tank 1, and connecting the light-transmitting pipe 22 to the fermentation tank by using the screw clamp connector 221 and the connection pipe 222 having an external screw thread.
Utilize threaded connection to make things convenient for the dismouting of printing opacity pipe 22, thereby the printing opacity decline is adhered to the fermentation material to long-time use printing opacity pipe 22, through the later stage dismantlement of being convenient for of helicitic texture washing.
Example 4: for the problem that a part of fermented substances may have large fermented impurities, a filter screen 4 is arranged at the front end of a micro pump installed on a light transmission pipe 22 as shown in fig. 4, and the filter screen is used for filtering the large impurities, so that the interference during detection is reduced.
The basic principles, main features and advantages of the present invention have been described above. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the principles of the present invention may be applied to any other embodiment without departing from the spirit and scope of the present invention.
Claims (6)
1. A microbial fermentation detection device comprises a fermentation tank (1) and a detection device (2), wherein the detection device (2) is arranged on the side wall of the fermentation tank (1), and is characterized in that the detection device (2) comprises a light source (21), a light transmission tube (22), a photoelectric detector (23), a shell (24) and an operation panel (25), the shell (24) is arranged on the side wall of the fermentation tank (1), the light source (21), the light transmission tube (22) and the photoelectric detector (23) are arranged in the shell (24), and the operation panel (25) is arranged on the surface of the shell (24);
wherein the light source (21) is tightly attached to the side wall of the fermentation tank (1), the light transmission tube (22) is arranged on the outer side of the light source (21), the upper end and the lower end of the light transmission tube (22) are communicated with the interior of the fermentation tank (1) through bent tubes, and the photoelectric detector (23) is arranged on the outer side of the light transmission tube (22) and located on the inner side surface of the shell (24).
2. The apparatus for detecting the fermentation of microorganisms according to claim 1, wherein a micro pump (3) is installed on the bent pipe at the lower end of the light-transmitting pipe (22).
3. The microorganism fermentation detection device according to claim 1, wherein the ends of the bent pipes at the two ends of the light-transmitting pipe (22) are provided with threaded clamping joints (221), the fermentation tank is provided with an upper connecting pipe (222) and a lower connecting pipe (222), the ends of the connecting pipes (222) are provided with external threads, and the light-transmitting pipe is installed on the fermentation tank through the threaded clamping joints (221) and the connecting pipes (222).
4. The microorganism fermentation detection device according to claim 1, wherein a filter screen (4) is arranged at the connection part of the lower end of the light transmission tube (22) and the fermentation tank (1).
5. The microbial fermentation detection device of claim 1, wherein the light-transmitting tube (22) is a high-light-transmitting glass tube, and bent tubes are mounted at two ends of the glass tube to form a U-shaped tube structure.
6. The apparatus of claim 1, wherein a desiccant is disposed in the housing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120542114.4U CN215209345U (en) | 2021-03-16 | 2021-03-16 | Microbial fermentation detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120542114.4U CN215209345U (en) | 2021-03-16 | 2021-03-16 | Microbial fermentation detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215209345U true CN215209345U (en) | 2021-12-17 |
Family
ID=79442868
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120542114.4U Expired - Fee Related CN215209345U (en) | 2021-03-16 | 2021-03-16 | Microbial fermentation detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215209345U (en) |
-
2021
- 2021-03-16 CN CN202120542114.4U patent/CN215209345U/en not_active Expired - Fee Related
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|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211217 |