CN214032535U

CN214032535U - Novel cell culture device

Info

Publication number: CN214032535U
Application number: CN202022562689.0U
Authority: CN
Inventors: 林俊堂; 付康; 王现伟; 杨远方; 亢星亮; 任新华
Original assignee: Henan Intercell Biology Technology Co ltd
Current assignee: Henan Intercell Biology Technology Co ltd
Priority date: 2020-11-09
Filing date: 2020-11-09
Publication date: 2021-08-24
Anticipated expiration: 2030-11-09

Abstract

The utility model relates to a novel cell culture device, which relates to the field of cell culture and comprises an incubator with an opening at the top end and a case cover buckled at the top end of the incubator, wherein the top surface of the incubator is provided with an annular groove which is coaxially arranged with the incubator, the inner wall of the top part of the incubator is provided with a first through hole which is communicated with the annular groove, the outer end of the first through hole is communicated with a first liquid inlet pipeline which is horizontally arranged, the inner end of the first through hole is communicated with a second liquid inlet pipeline which is horizontally arranged, a first sealing element is arranged in the free end of the first liquid inlet pipeline, the bottom surface of the case cover is provided with an annular clamping plate which is matched with the annular groove in shape, the side wall of the annular clamping plate is provided with a second through hole which has the same aperture as the first through hole and corresponds to the position, the second through hole is positioned below the first through hole after the annular clamping plate is inserted into the annular groove, the utility model can avoid the secondary pollution to the cells in the incubator when adding cell nutrient solution into the incubator, further reducing cell contamination.

Description

Novel cell culture device

Technical Field

The utility model belongs to the technical field of cell culture, especially, relate to a novel cell culture device.

Background

Cell culture techniques, also called cell cloning techniques, are the most central and basic techniques in biotechnology because of the large number of cells or their metabolites that can be obtained by cell culture, and the biological products are all derived from cells.

The cell growth needs a nutrient environment, the nutrient medium for maintaining the cell growth can be divided into a liquid state and a solid state according to the physical state, at present, the cell culture is generally carried out by adding the liquid nutrient medium, namely cell nutrient solution, into an incubator or a culture bottle, the cell nutrient solution is added into the incubator by the existing cell incubator by arranging through holes on the inner walls of the two sides of the incubator, a rubber plug is arranged in the through holes, the cell nutrient solution is added into the incubator by a nutrient solution adder after the rubber plug is pulled out, the nutrient solution adder is pulled out and the rubber plug is plugged back after the addition is finished, but the incubator is communicated with the outside twice by the method, the pulling out of the rubber plug cannot be avoided in order to add the cell nutrient solution into the incubator, but a technical scheme can be designed to firstly cut off the communication between the incubator and the outside before the rubber plug is plugged back after the addition of the cell nutrient solution is finished, and then the nutrient solution adder is pulled out, and the rubber plug is plugged back to avoid the secondary pollution of cells, but the existing cell culture box can not realize the functions, so the prior art has the defects and shortcomings.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a novel cell culture device solves the problem that current cell culture case can be to cell secondary pollution when adding cell nutrient solution.

In order to solve the problem, the utility model discloses the technical scheme who takes:

the utility model provides a novel cell culture device, includes open-top's incubator and the case lid of lock on the incubator top, its characterized in that: the utility model discloses a seal box, including incubator top, first through-hole, first liquid inlet pipeline, case lid bottom surface, set up the annular groove that sets up with the incubator coaxial line on the incubator top inner wall, first through-hole and annular groove intercommunication, first liquid inlet pipeline that first through-hole outer end intercommunication has the level to set up, the second liquid inlet pipeline that the inner intercommunication of first through-hole has the level to set up, be provided with first sealing member in the first liquid inlet pipeline free end, the case lid bottom surface is provided with the annular cardboard with annular groove shape adaptation, set up the second through-hole that corresponds with the same aperture of first through-hole and position on the annular cardboard inner wall, the annular cardboard inserts the below of annular groove back second through-hole site in first through-hole.

Further, second inlet conduit body bottom intercommunication has the liquid pipeline that divides of a plurality of equidistance distribution, it is perpendicular with second inlet conduit to divide the liquid pipeline, the quantity of first through-hole and second through-hole is two, and two first through-holes and two second through-holes all set up about incubator axis symmetry.

Furthermore, the outer wall of the annular clamping plate is carved with a marking line, detachable supporting rods are arranged between the bottom surface of the incubator cover and the top surface of the incubator, the number of the supporting rods is at least two, and the height of each supporting rod is equal to the distance between the marking line and the bottom surface of the incubator cover.

Further, a plurality of equally spaced ring groove that distributes is seted up respectively by the top on the bottom along incubator circumference on the incubator inner wall, still be provided with the layer board the same with ring groove quantity and shape adaptation in the incubator, layer board circumference edge is located corresponding ring groove, has the clearance between layer board circumference edge and the corresponding ring groove lateral wall, and layer board bottom surface butt has the clearance between layer board top surface and the ring groove top surface on the ring groove bottom surface, all is provided with the connecting rod of vertical setting between per two adjacent layer boards, is located the thread extension piece of the vertical setting of first layer board top fixedly connected with at incubator top, thread extension piece top has the handle after passing the case lid, handle bottom surface and case lid top surface butt.

Further, the thread telescopic piece comprises a threaded rod and a thread sleeve sleeved on the threaded rod, the thread sleeve is in threaded connection with the threaded rod, the top end of the thread sleeve penetrates through the box cover and then is in threaded connection with the handle, and the bottom end of the threaded rod is fixedly connected with the top of a first supporting plate located at the top of the incubator.

Furthermore, the screwing direction of the threaded connection of the threaded sleeve and the threaded rod is opposite to the screwing direction of the threaded connection of the threaded sleeve and the handle.

Furthermore, the incubator is internally provided with guide boxes corresponding to the number of the annular clamping grooves, two ends of each guide box are open, the horizontal cross section area of the top surface of each guide box is larger than that of the bottom surface of each guide box, and the top surfaces of the guide boxes are fixedly connected with the bottom surfaces of the annular clamping grooves.

Furthermore, the inner walls of the incubators on the two sides of the flow guide box are provided with third through holes, and second sealing pieces are arranged in the third through holes.

Further, second sealing member and first sealing member are the rubber buffer.

Adopt above-mentioned technical scheme, the beneficial effects of the utility model are that:

the utility model realizes the buckling of the incubator and the incubator cover by arranging the annular groove on the top surface of the incubator and the annular clamping plate on the bottom surface of the incubator cover, arranging the first through hole communicated with the annular groove on the inner wall of the incubator, arranging the second through hole on the annular clamping plate, wherein the two ends of the first through hole are respectively communicated with the first liquid inlet pipeline and the second liquid inlet pipeline, when adding cell nutrient solution into the incubator, lifting the incubator cover to ensure that the first liquid inlet pipeline, the first through hole, the second through hole and the second liquid inlet pipeline form a channel for conveying the cell nutrient solution together, after pulling out the first sealing element, adopting the nutrient solution adder to add the cell nutrient solution into the incubator, putting down the incubator cover after adding, firstly disconnecting the inside of the incubator from the outside, then pulling out the nutrient solution adder, plugging back the first sealing element, avoiding the secondary pollution of the incubator to cells when adding the cell nutrient solution, further reducing cell contamination; through set up the layer board that a plurality of equidistance distributes in the incubator, the cardinal number of the interior cultured cell of increase incubator, the cell nutrient solution that still is convenient for circulate between each layer board.

Drawings

Fig. 1 is one of the schematic structural diagrams of the present invention;

FIG. 2 is a schematic view of the structure of the cover of the incubator of the present invention not fastened to the incubator;

fig. 3 is a second schematic structural diagram of the present invention.

Reference numerals: 1. an incubator; 11. an annular groove; 12. a first through hole; 13. an annular neck; 14. a third through hole; 15. a second seal member; 2. a box cover; 21. an annular clamping plate; 22. a second through hole; 23. a support bar; 3. a first liquid inlet pipe; 4. a second liquid inlet pipe; 41. a liquid separation pipeline; 5. a support plate; 6. a connecting rod; 7. a threaded telescoping member; 71. a threaded rod; 72. a threaded bushing; 8. a handle; 9. a diversion box.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, embodiments of the present invention are described in further detail below with reference to the accompanying drawings.

As shown in figures 1 to 3, the utility model provides a novel cell culture device, which comprises an incubator 1 with an open top end and a cover 2 fastened on the top end of the incubator 1, wherein the cover 2 is sealed and ventilated to facilitate the circulation and the conversion of cell gas in the incubator 1, the top surface of the incubator 1 is provided with an annular groove 11 coaxial with the incubator 1, the inner wall of the top of the incubator 1 is provided with a first through hole 12, the first through hole 12 is communicated with the annular groove 11, the outer end of the first through hole 12 is communicated with a horizontally arranged first liquid inlet pipeline 3, the inner end of the first through hole 12 is communicated with a horizontally arranged second liquid inlet pipeline 4, the free end of the first liquid inlet pipeline 3 is internally provided with a first sealing element, the bottom surface of the cover 2 is provided with an annular clamping plate 21 matched with the shape of the annular groove 11, the inner wall of the annular clamping plate 21 is provided with a second through hole 22 with the same aperture, after the annular clamping plate 21 is inserted into the annular groove 11, the second through hole 22 is positioned below the first through hole 12 to disconnect the communication between the inside of the incubator 1 and the outside, the annular groove 11 is arranged on the top surface of the incubator 1 and the annular clamping plate 21 is arranged on the bottom surface of the incubator 2 to realize the buckling of the incubator 1 and the incubator cover 2, the first through hole 12 communicated with the annular groove 11 is arranged on the inner wall of the incubator 1, the second through hole 22 is arranged on the annular clamping plate 21, the two ends of the first through hole 12 are respectively communicated with the first liquid inlet pipeline 3 and the second liquid inlet pipeline 4, as shown in figure 3, when adding cell nutrient solution into the incubator 1, the incubator cover 2 is lifted to enable the first liquid inlet pipeline 3, the first through hole 12, the second through hole 22 and the second liquid inlet pipeline 4 to jointly form a channel for conveying the cell nutrient solution, after pulling out the first sealing element, a nutrient solution adder is adopted to add the cell nutrient solution into the incubator 1, after the cell nutrient solution is added, the box cover 2 is put down, the communication between the inside of the incubator 1 and the outside is disconnected at first, and then the nutrient solution adder is pulled out and plugged back to the first sealing element, so that the secondary pollution of the incubator 1 to cells when the cell nutrient solution is added is avoided, and the cell pollution is further reduced.

In order to ensure that the cell nutrient solution can uniformly drip when the cell nutrient solution is added into the incubator 1 and avoid direct impact of overlarge cell nutrient solution on cells in the incubator 1, therefore, as shown in fig. 1 and fig. 3, the bottom of the second liquid inlet pipe 4 is connected to a plurality of liquid separating pipes 41 distributed at equal intervals, the liquid separating pipes 41 are perpendicular to the second liquid inlet pipe 4, that is, the liquid separating pipes 41 are arranged vertically, the liquid separating pipes 41 are used for dropping the cell nutrient solution flowing through the second liquid inlet pipe 4 into the incubator 1 uniformly, in addition, the number of the first through holes 12 and the second through holes 22 is two, the two first through holes 12 and the two second through holes 22 are arranged symmetrically with respect to the axis of the incubator 1, with this arrangement, the time for adding the cell nutrient solution can be shortened, and the cell nutrient solution can be further uniformly added into the incubator 1.

For further accelerate first inlet channel 3, first through-hole 12, form the time of carrying cell nutrient solution passageway jointly between second through-hole 22 and the second inlet channel 4, therefore, as shown in fig. 3, be carved with the mark line on the annular cardboard 21 outer wall, the mark line is the mark line when case lid 2 promotes to target in place, be provided with detachable bracing piece 23 between case lid 2 bottom surface and incubator 1 top surface, bracing piece 23 quantity is two at least, the height of bracing piece 23 equals the distance between mark line and case lid 2 bottom surface, through setting up the mark line, when the height that promotes case lid 2 reaches the mark line position, stand bracing piece 23 between case lid 2 and incubator 1 and guarantee the stability of cell nutrient solution passageway, accelerate cell nutrient solution passageway formation time.

In order to increase the base number of cells cultured in the incubator 1, as shown in fig. 1 to 3, a plurality of annular clamping grooves 13 are respectively arranged on the inner wall of the incubator 1 from top to bottom along the circumferential direction of the incubator 1, the incubator 1 is further provided with supporting plates 5 which are the same in number and shape as the annular clamping grooves 13, specifically, the supporting plates 5 are arranged to increase the base number of adherent cells cultured in the incubator 1, in addition, when cell nutrient solution is added into the incubator 1, nutrient solution is convenient to circulate between the supporting plates 5, therefore, the circumferential edges of the supporting plates 5 are positioned in the corresponding annular clamping grooves 13, gaps are arranged between the circumferential edges of the supporting plates 5 and the side walls of the corresponding annular clamping grooves 13, the bottom surfaces of the supporting plates 5 are abutted against the bottom surfaces of the annular clamping grooves 13, gaps are arranged between the top surfaces of the supporting plates 5 and the annular clamping grooves 13, and a connecting rod 6 which is vertically arranged between every two adjacent supporting plates 5, the screw thread extensible member 7 that is located the vertical setting of 5 top fixedly connected with of first layer board at 1 top of incubator, screw thread extensible member 7 top is threaded connection has handle 8 after passing case lid 2, handle 8 bottom surface and 2 top surfaces butts of case lid, through such mode of setting, lift each layer board 5 through handle 8 after case lid 2 promotes, thereby can make 13 circulation cell nutrient solutions of ring groove, if the height that stretches out of screw thread extensible member 7 is adjustable its height inadequately, promote with adaptation layer board 5.

As shown in fig. 1 and 3, the specific structure and adjustment mode of the threaded expansion piece 7 are as follows: the threaded telescopic part 7 comprises a threaded rod 71 and a threaded sleeve 72 sleeved on the threaded rod 71, the threaded sleeve 72 is in threaded connection with the threaded rod 71, the top end of the threaded sleeve 72 penetrates through the box cover 2 to be in threaded connection with a handle 8, the bottom end of the threaded rod 71 is fixedly connected with the top of a first supporting plate 5 positioned at the top of the incubator 1, the box cover 5 is buckled with the incubator 1, the threaded sleeve 72 is completely sleeved on the threaded rod 71, if the box cover 2 needs to be lifted, the threaded sleeve 72 is rotated to adjust the extension height of the threaded telescopic part 7 to a proper position, the box cover 2 is lifted, the supporting plate 5 is lifted after positioning, the threaded sleeve 72 and the handle 8 are rotated until the bottom of the handle 8 and the top surface of the box cover 2 are abutted against and fixed.

Further, in order to adjust the extending height of the threaded telescopic element 7 and the distance between the handle 8 and the top surface of the box cover 2, the threaded sleeve 72 is screwed with the threaded rod 71 in the opposite direction to the threaded sleeve 72 screwed with the handle 8.

As shown in fig. 1 to 3, the cell nutrient solution flowing out of the ring slot 13 cannot completely drop on each support plate 5, therefore, the inside 1 of the incubator is further provided with the diversion boxes 9 corresponding to the number of the ring slots 13, both ends of the diversion boxes 9 are open, the horizontal cross-sectional area of the top surface of the diversion box 9 is larger than that of the bottom surface of the diversion box 9, the top surface of the diversion box 9 is fixedly connected with the bottom surface of the ring slot 13, the lifting of each support plate 5 is not affected by the arrangement of the diversion boxes 9, and the diversion boxes 9 are used for completely dropping the cell nutrient solution flowing through the ring slots 13 onto the support plates 5, thereby facilitating the growth of cells.

As shown in fig. 1 to 3, since the support plate 5 and the baffle box 9 are arranged in the incubator 1, cells in the incubator 1 cannot be sucked out by using a sampler, therefore, the inner walls of the incubator 1 at the two sides of the baffle box 9 are all provided with the third through holes 14, the third through holes 14 are all provided with the second sealing elements 15, and the cells in the incubator 1 are sucked out by using the sampler by setting the third through holes 14, thereby facilitating the utilization of the cells.

Further, second sealing member 15 and first sealing member are the rubber buffer, because the material of incubator 1 adopts glass or plastics mostly, consequently, first sealing member and second sealing member 15 can realize sealing to first feed liquor pipeline 3 and third through-hole 14 for the rubber buffer.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, but rather is described in the embodiments and descriptions herein to illustrate the principles of the invention and that various changes and modifications may be made without departing from the spirit and scope of the invention, all of which fall within the scope of the claimed invention. The scope of the invention is defined by the appended claims and equivalents.

Claims

1. The utility model provides a novel cell culture device, includes top open-ended incubator (1) and case lid (2) of lock on incubator (1) top, its characterized in that: the top surface of the incubator (1) is provided with an annular groove (11) which is coaxial with the incubator (1), the inner wall of the top of the incubator (1) is provided with a first through hole (12), the first through hole (12) is communicated with the annular groove (11), the outer end of the first through hole (12) is communicated with a first liquid inlet pipeline (3) which is horizontally arranged, the inner end of the first through hole (12) is communicated with a second liquid inlet pipeline (4) which is horizontally arranged, a first sealing element is arranged in the free end of the first liquid inlet pipeline (3), an annular clamping plate (21) matched with the annular groove (11) in shape is arranged on the bottom surface of the box cover (2), and a second through hole (22) which has the same aperture as the first through hole (12) and corresponds to the first through hole in position is formed in the inner wall of the annular clamping plate (21), and the second through hole (22) is positioned below the first through hole (12) after the annular clamping plate (21) is inserted into the annular groove (11).

2. A novel cell culture apparatus according to claim 1, wherein: second inlet channel (4) body bottom intercommunication has liquid pipeline (41) that a plurality of equidistance distributes, it is perpendicular with second inlet channel (4) to divide liquid pipeline (41), the quantity of first through-hole (12) and second through-hole (22) is two, and two first through-holes (12) and two second through-holes (22) all set up about incubator (1) axis symmetry.

3. A novel cell culture apparatus according to claim 1, wherein: the outer wall of the annular clamping plate (21) is carved with a marking line, detachable supporting rods (23) are arranged between the bottom surface of the box cover (2) and the top surface of the incubator (1), the number of the supporting rods (23) is at least two, and the height of each supporting rod (23) is equal to the distance between the marking line and the bottom surface of the box cover (2).

4. A novel cell culture apparatus according to claim 3, wherein: a plurality of annular clamping grooves (13) which are distributed equidistantly are respectively formed in the inner wall of the incubator (1) from top to bottom along the circumferential direction of the incubator (1), supporting plates (5) which are the same in number as the annular clamping grooves (13) and are adaptive in shape are further arranged in the incubator (1), the circumferential edges of the supporting plates (5) are positioned in the corresponding annular clamping grooves (13), gaps are formed between the circumferential edges of the supporting plates (5) and the side walls of the corresponding annular clamping grooves (13), the bottom surfaces of the supporting plates (5) are abutted against the bottom surfaces of the annular clamping grooves (13), gaps are formed between the top surfaces of the supporting plates (5) and the top surfaces of the annular clamping grooves (13), a vertically-arranged connecting rod (6) is arranged between every two adjacent supporting plates (5), a vertically-arranged thread telescopic piece (7) is fixedly connected to the top of a first supporting plate (5) at the top of the incubator (1), and a lifting handle (8) is connected to the top end of the thread telescopic piece (7) after passing through the incubator cover (2), the bottom surface of the handle (8) is abutted against the top surface of the box cover (2).

5. A novel cell culture device according to claim 4, wherein: screw thread extensible member (7) include threaded rod (71) and establish screw sleeve pipe (72) on threaded rod (71) with the cover, screw sleeve pipe (72) and threaded rod (71) threaded connection, screw sleeve pipe (72) top is passed case lid (2) back threaded connection handle (8), threaded rod (71) bottom and the first layer board (5) top fixed connection that is located incubator (1) top.

6. A novel cell culture apparatus according to claim 5, wherein: the screwing direction of the threaded connection of the threaded sleeve (72) and the threaded rod (71) is opposite to that of the threaded connection of the threaded sleeve (72) and the handle (8).

7. A novel cell culture device according to claim 4, wherein: the incubator (1) is also internally provided with flow guide boxes (9) corresponding to the number of the ring-shaped clamping grooves (13), the two ends of each flow guide box (9) are opened, the horizontal cross-sectional area of the top surface of each flow guide box (9) is larger than that of the bottom surface of each flow guide box (9), and the top surface of each flow guide box (9) is fixedly connected with the bottom surface of each ring-shaped clamping groove (13).

8. The novel cell culture device according to claim 7, wherein: third through holes (14) are formed in the inner walls of the incubators (1) on the two sides of the flow guide box (9), and second sealing pieces (15) are arranged in the third through holes (14).

9. A novel cell culture apparatus according to claim 8, wherein: the second sealing element (15) and the first sealing element are rubber plugs.