CN213995930U - Liquid transfer device - Google Patents

Abstract

A liquid transfer device comprises a liquid collection container with a liquid injection port part and a sample injection port part, a one-way sealing element arranged in the end part of the liquid collection container adjacent to the sample injection port part, and a movable sealing element inserted in the liquid collection container through the liquid injection port part. The inner space of the liquid collecting container is divided into one part with a sample adding opening part and the other part with a liquid injection opening part by the arranged one-way sealing piece; after the liquid is collected by the liquid injection opening part, the liquid can be sealed in the liquid collection container through the plugging action of the movable sealing piece on the liquid injection opening part; when the movable sealing element is pushed and pressed, the liquid in the liquid collecting container can be driven to jack the one-way sealing element, so that the liquid is discharged from the sample adding opening part, and the liquid sample adding is realized; this liquid transfer device collects, preserves and function collection such as application of sample with liquid as an organic whole, can effectively save the use quantity of relevant matching equipment, accomplishes liquid transfer and deposits more conveniently, swiftly.

Description

Liquid transfer device

Technical Field

The utility model relates to an experiment detection equipment technical field, concretely relates to liquid transfer device.

Background

In the fields of biology, medicine, chemistry, etc., it is known that a series of operations such as sampling, storing, transferring, and loading of a liquid such as a sample solution and a reagent are often required to complete a corresponding experiment or test item. Wherein, the liquid transferring operation is mainly completed by a liquid transferring device (such as a micro liquid transferring gun, etc.), namely: transferring a fixed amount of liquid from the original container to another container by using a pipetting device. However, most of the prior liquid-transferring devices only have a single function of transferring liquid, and cannot separately collect, store, sample, and add sample to the liquid, and often need to complete a series of operations on the liquid in cooperation with other devices such as a storage tube and a pipette, thereby increasing the number of configuration of related experimental devices and reducing the efficiency of experiments or detection.

SUMMERY OF THE UTILITY MODEL

The utility model discloses the main technical problem who solves provides a liquid transfer device to reach the purpose of richening practical function.

In one embodiment there is provided a liquid transfer device comprising:

the liquid collection container is provided with a liquid injection port part and a sample injection port part, and the liquid injection port part is used for injecting liquid into the liquid collection container;

a one-way seal disposed within an end of the liquid collection container adjacent the sample addition port portion; and

the movable sealing piece is inserted into the liquid collecting container through the liquid injection opening part and used for plugging the liquid injection opening part and driving liquid to pass through the one-way sealing piece and then be discharged out of the liquid collecting container through the sample injection opening part.

In one embodiment, the outer peripheral wall of the sample loading port part is provided with air pressure balance grooves which are distributed along the extension direction of the sample loading port part.

In one embodiment, the one-way seal has a sealing cover portion having a contour shape matching a cross-sectional shape of an end of the liquid collection container adjacent the sample addition port portion;

the sealing cover is provided with a non-return slot, the shape of the section of the non-return slot is triangular, and the notch end of the non-return slot is positioned on the surface of the sealing cover facing one side of the liquid injection port.

In one embodiment, the movable seal comprises:

the plug part is used for plugging the liquid injection opening part, and the plug part is coaxially inserted into the liquid collection container;

the plug rod part is used for driving the plug part to be close to or far away from the one-way sealing element in the liquid collecting container so as to drive liquid to pass through the one-way sealing element, and the plug rod part is connected with one end, far away from the one-way sealing element, of the plug part.

In one embodiment, the movable sealing element further comprises a flexible connecting belt, one end of the flexible connecting belt is connected with the plug rod part, and the other end of the flexible connecting belt is connected with the liquid collecting container.

In one embodiment, the liquid collection container comprises:

the liquid sample adding pipe is provided with a sample adding opening part formed at one end of the liquid sample adding pipe, and a sleeve connecting part is formed at the other end of the liquid sample adding pipe; and

the liquid inlet part is formed at one end of the liquid collecting pipe, and the liquid outlet part is formed at the other end of the liquid collecting pipe;

the liquid collecting pipe is connected with the liquid sample adding pipe plug bush through the sleeve interface part, so that the liquid outlet part is positioned in the liquid sample adding pipe and is coaxially opposite to the sample adding opening part; the one-way sealing element is positioned in the liquid sample adding pipe and clamped between the sample adding opening part and the liquid outlet part, and the movable sealing element is inserted in the liquid collecting pipe.

In one embodiment, the liquid outlet part comprises a conical pipe part and a port pipe part, the main body of the liquid collecting pipe is a tubular structure formed by axially extending the conical bottom end of the conical pipe part, and the port pipe part is a tubular structure formed by axially extending the conical top end of the conical pipe part; the one-way sealing element covers one end of the port pipe part far away from the conical pipe part.

In one embodiment, the inner peripheral wall of the liquid sample adding pipe adjacent to the sample adding opening part is provided with a pressure-bearing flange distributed around the sample adding opening part, and the pressure-bearing flange is used for abutting against a one-way sealing element; the outer peripheral wall of the liquid collecting pipe is provided with a limiting flange, and the limiting flange is used for abutting against a port face of the sleeve connecting part.

The liquid transfer device according to the above embodiment includes a liquid collection container having a liquid injection port portion and a sample injection port portion, a one-way seal member provided in an end portion of the liquid collection container adjacent to the sample injection port portion, and a movable seal member inserted into the liquid collection container via the liquid injection port portion. The inner space of the liquid collecting container is divided into one part with a sample adding opening part and the other part with a liquid injection opening part by the arranged one-way sealing piece; after the liquid is collected by the liquid injection opening part, the liquid can be sealed in the liquid collection container through the plugging action of the movable sealing piece on the liquid injection opening part; when the movable sealing element is pushed and pressed, the liquid in the liquid collecting container can be driven to jack the one-way sealing element, so that the liquid is discharged from the sample adding opening part, and the liquid sample adding is realized; this liquid transfer device collects, preserves and function set such as application of sample with liquid is as an organic whole, can draw the consumptive material with the nucleic acid is automatic to match and use, also can be used to such as collection, the preservation and the application of sample of liquid such as reagent, biological sample solution, can effectively save the use quantity of relevant matching equipment, and more convenient, swiftly accomplish liquid and shift and deposit, created the advantage for the efficiency that improves experiment or detect.

Drawings

FIG. 1 is a schematic sectional view of a liquid transfer device according to an embodiment;

FIG. 2 is an exploded view of an embodiment of a liquid transfer device;

FIG. 3 is an exploded view of an embodiment of a fluid transfer device with a movable seal removed;

fig. 4 is an exploded view of the liquid transfer device according to an embodiment after the movable seal is removed (ii).

In the figure:

10. a one-way seal; 11. a sealing cover portion; 12. a seal housing portion; 20. a movable seal; 21. a plug portion; 22. a stopper rod portion; 23. a soft connection portion; 24. a ring groove is embedded; 30. a liquid sample addition tube; 31. a pressure-bearing flange; 40. a liquid collection tube; 41. a limiting flange;

a. a liquid injection port; b. a sample addition port part; c. an air pressure balancing tank; d. a check slot; e. a sleeve interface part; f. a liquid outlet part; f1, a tapered tube portion; f2, port tube part.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

The liquid transfer device provided by the application can be matched with nucleic acid automatic extraction consumables (such as downstream instruments like sample test tubes or upstream instruments like sampling needles) for use, and can also be used for collecting, storing and sample adding of liquids such as reagents and biological sample solutions; the inner space of the liquid collecting container is divided into one part with a sample adding opening part and the other part with a liquid injection opening part by the arranged one-way sealing piece, when liquid is collected, the liquid in an upstream appliance can be added into the liquid collecting container through the liquid injection opening part by adopting a negative pressure liquid injection mode, and the liquid injection opening part is sealed by utilizing the movable sealing piece, so that the collection and the storage of the liquid are realized; when liquid is added, the liquid can be butted and communicated with a downstream appliance by using the sample adding opening part, and the liquid enters the downstream appliance through the sample adding opening part after passing through the one-way sealing element by pushing the movable sealing element so as to realize the sample adding of the liquid; compared with the existing liquid transferring equipment such as a liquid transfer device, the liquid transferring device integrates the functions of liquid collection, storage, sample adding and the like into a whole, can effectively save the using quantity of relevant matched equipment in the liquid transferring and temporary storage process, can more conveniently and quickly complete liquid transfer and storage, and creates favorable conditions for improving the efficiency of experiments or detection.

Referring to fig. 1, 2, 3 and 4, one embodiment provides a liquid transfer device comprising a one-way seal 10, a movable seal 20, and a liquid collection container consisting of a liquid sample application tube 30 and a liquid collection tube 40; wherein:

the liquid sample adding pipe 30 is mainly used for matching with a sample inlet of a downstream container so as to add the liquid stored in the liquid collecting pipe 40 into the downstream container; the liquid sample application tube 30 has a sample application port b formed at one end thereof for use as a component for abutting and communicating with a downstream container, and a socket e formed at the other end of the liquid sample application tube 30 for use as a component for assembling and connecting the liquid sample application tube 30 and the liquid collection tube 40.

The liquid collecting pipe 40 is mainly used for matching with a sample outlet of an upstream container so as to collect and store liquid discharged by the upstream container; a liquid inlet a is formed at one end of the liquid collecting pipe 40 and is used as a structural component which is in butt joint communication with a sample outlet of an upstream container, and a liquid outlet f is formed at the other end of the liquid collecting pipe 40 and is used as a pipeline for discharging liquid from the liquid collecting pipe 40; the liquid collecting pipe 40 and the liquid outlet part f are coaxially inserted into the liquid sample adding pipe 30 through the sleeve interface part e, so that the liquid outlet part f is positioned in the liquid sample adding pipe 30, and the liquid outlet part f and the sample adding part b are coaxially and oppositely distributed; thus, by the close fitting relation between the liquid collecting tube 40 and the liquid sample application tube 30, a sealed space can be formed between the outer peripheral wall of the liquid collecting tube 40 and the inner peripheral wall of the liquid sample application tube 30, and the liquid outlet portion f is positioned in the sealed space. In this embodiment, the liquid sampling tube 30 and the liquid collecting tube 40 both adopt tubular structures with circular radial cross-sectional shapes, so that the tight insertion and sleeving connection relationship between the two is enhanced, and the two can be conveniently disassembled, assembled and maintained. In another embodiment, the liquid sampling tube 30 and the liquid collecting tube 40 may be tubular structures having polygonal cross-sectional shapes, wherein: can guarantee that both can dismantle the connection, and after the assembly, can form sealed space between the two.

The one-way sealing member 10 mainly covers the liquid outlet part f to prevent the leakage of liquid and gas, and the one-way sealing member 10 can divide the inner space of the liquid collecting container into one part with the sample inlet part b and the other part with the liquid inlet part a (namely, the liquid collecting pipe 40 and the liquid sample adding pipe 30 are separated); when air is pumped from one side of the liquid injection port part a, the one-way sealing element 10 can prevent external air from entering the liquid collection pipe 40 from one side of the sample injection port part b; when liquid is loaded, the one-way sealing element 10 can be opened under the action of liquid pressure so as to load the liquid into the downstream container through the loading port part b; in this embodiment, the one-way sealing element 40 is made of rubber material, and a notch structure that can be opened under a certain liquid pressure to allow liquid to pass through is formed on the one-way sealing element 40. In other embodiments, the one-way seal 40 may also be structurally selected or designed with reference to existing one-way check valves, the point being: the valve can be opened only under a certain pressure and a constant pressure direction.

The movable sealing element 20 is inserted into the liquid collecting pipe 40 through the liquid filling opening part a, and the movable sealing element 20 is provided with a structural part of which the outer peripheral wall is tightly attached to the inner peripheral wall of the liquid collecting pipe 40 and the inner peripheral wall of the liquid filling opening part a; firstly, a sealed space isolated from the outside is formed in the liquid collecting pipe 40 by sealing the liquid injection opening part a and matching a sealing cover of the one-way sealing element 10 to the liquid outlet part f so as to contain and preserve liquid; secondly, the movable sealing member 40 moves in the liquid collecting pipe 40 towards the liquid outlet f by applying a squeezing force to the movable sealing member 20, so that the unidirectional sealing member 10 is opened by the pressure generated by the liquid, the liquid is driven to pass through the unidirectional sealing member 10 and then is discharged through the sample inlet b, and the liquid is further added into the downstream container.

In one aspect, the liquid transfer device is used in conjunction with an upstream container means to collect liquid; the liquid outlet of the upstream container can be nested in the liquid injection port part a, and the air suction port of the upstream container is positioned in the liquid injection port part a, so that a relatively sealed structural space can be formed in the liquid collecting pipe 40 by utilizing the plugging of the upstream container to the liquid injection port part a and the plugging of the one-way sealing element 10 to the liquid outlet port part f; when the upstream container is pumped, the liquid in the upstream container is guided into the structural space by the negative pressure effect generated in the structural space, so that the liquid collection is completed by the liquid collection pipe 40; after the upstream container is separated from the liquid filling opening part a, the movable sealing element 20 can be inserted into the liquid collecting pipe 40, so that the liquid is sealed in the liquid collecting pipe 40, and the collected liquid is convenient to store for a certain time or leak in the liquid transferring process.

On the other hand, when the liquid transfer device is matched with a downstream container tool for use so as to sample liquid; the sample adding port of the downstream container can be in plug-and-socket butt joint communication with the sample adding port part b, and then the movable sealing piece 20 is pushed to move towards the direction of the one-way sealing piece 10 in the liquid collecting pipe 40, so that the one-way sealing piece 10 can be pushed open by liquid, and the liquid can smoothly flow into the downstream container through the sample adding port part b, and the process of adding the liquid is finished.

In some embodiments, the liquid collection container may also be composed of only the liquid collection tube 40, and the liquid outlet portion f is used as the sample inlet portion b, in which case, the one-way sealing member 10 may be fixed and covered on the outer port side of the liquid outlet portion f by means such as ultrasonic welding, or the one-way sealing member 40 may be fixed in the end portion of the liquid collection tube 40 adjacent to the liquid outlet portion f by means such as clamping, welding, or the like; in this way, the structure of the entire liquid transfer apparatus can be simplified and the number of component parts can be reduced.

In one embodiment, referring to fig. 1 and 3, an air pressure balance groove c is disposed on the outer peripheral wall of the sample addition part b, and the air pressure balance grooves c are distributed along the extending direction of the sample addition part b. Under the condition that the aperture size of the sample injection port of the downstream container is matched with the aperture size of the sample injection port part b, so that the sample injection port part b is in butt joint communication with the sample injection port of the downstream container in a plug-in mounting mode, a structural gap can be formed between the outer peripheral wall of the sample injection port part b and the inner wall of the sample injection port of the downstream container by using the air pressure balance groove c, so that the inner space of the downstream container can be communicated with the outside through the air pressure balance groove c, and therefore in the process of injecting liquid into the downstream container through the movable sealing element 20, the liquid can smoothly flow into the downstream container due to balance of internal and external air pressures; therefore, the adaptability and the universality of the whole liquid transfer device can be effectively improved without arranging an air pressure balance structure on the downstream container or selecting the downstream container with the air pressure balance structure or a component.

In one embodiment, referring to fig. 1 and fig. 4, the one-way sealing member 10 has a sealing cover portion 11 and a sealing sleeve portion 12 formed around the sealing cover portion 11, and the sealing sleeve portion 12 is sleeved on the liquid outlet portion f, so that the sealing cover portion 11 can completely cover and close the port of the liquid outlet portion f; meanwhile, a non-return slot d is formed in the sealing cover part 11, the shape of the section of the non-return slot d is triangular, and the notch end of the non-return slot d is positioned on the surface of the sealing cover part 11 facing the liquid injection port part a; therefore, a structural gap similar to the inner and outer structure or the upper and lower structure can be formed on the one-way sealing element 10 by utilizing the non-return slot d, when the liquid collecting pipe 40 is in a negative pressure air suction state, the external air of the liquid transfer device can be prevented from entering the liquid collecting pipe 40 through the sample adding opening part b, and the function effect of no air intake during air suction is realized; when liquid is added, the liquid pushes the non-return slot d open under the pressure of the movable sealing element 20, so that the liquid passes through the one-way sealing element 10. In this embodiment, the projected shape of the slot end of check slot d on the surface of one-way seal 10 may be cruciform, circular, elongated, etc.

In other embodiments, the one-way sealing member 10 may only have the sealing cover portion 11, and the contour shape of the sealing cover portion 11 matches with the port shape of the liquid outlet portion f, the port shape of the sample addition portion b, and the cross-sectional shape (or radial cross-sectional shape) of the liquid collection tube 40, so that the sealing cover portion 11 may be fixedly mounted by welding, clamping, and the like, and may also serve as a cover for the liquid outlet portion f or the sample addition portion b.

In one embodiment, referring to fig. 1 and 2, the movable sealing member 20 includes a plug portion 21, a stem portion 22, a sealing ring (not shown) and a soft connection portion 23; wherein:

the plug part 21 can be made of rubber material or has a peripheral wall made of rubber material, the shape and size of the plug part are matched with the liquid filling opening part a and the liquid collecting pipe 40, and the plug part 21 is coaxially inserted into the liquid collecting pipe 40 to realize the sealing of the liquid filling opening part a.

The stem portion 22 is connected to an end of the plug portion 21 away from the one-way sealing element 10, and mainly provides structural support for an operator to disassemble and operate the movable sealing element 40, so as to drive the plug portion 21 to move in the liquid collection tube 40 along an extending direction or an axial direction of the liquid collection tube 40 to approach or be away from the one-way sealing element 10, and further drive liquid to pass through the one-way sealing element 10 when moving towards the one-way sealing element 40.

The sealing ring is sleeved on the plug part 21 and clamped between the plug part 21 and the inner peripheral wall of the liquid collection container (specifically, the liquid collection pipe 40 or the liquid injection opening part a) so as to enhance the effect of close attachment of the movable sealing element 20 and the liquid collection container and realize the sealing of the liquid collection container; in this embodiment, the plug portion 21 can be sleeved with the sealing ring by using the ring groove 24 opened on the plug portion 21. In some embodiments, the sealing ring may also be omitted in case the plug portion 21 is able to maintain a tight fit with the inner wall of the liquid collection container.

The flexible connection belt 23 can be made of flexible or memory materials such as rubber, cable and the like, one end of the flexible connection belt 23 is connected with the plug rod part 22, and the other end of the flexible connection belt 23 is connected with a liquid collection container (specifically, a liquid collection pipe 40), so that the movable sealing element 20 and the liquid collection container can be paired and combined by using the flexible connection belt 23, the movable sealing element 20 can be conveniently disassembled and assembled at any time, and mutual pollution between liquids caused by accidental insertion into other liquid collection containers after the movable sealing element 20 is contaminated by the liquids can be prevented. In other embodiments, the flexible connecting strip 23 may be omitted to prevent incorrect mating of the movable seal 20 and the fluid collection container by providing corresponding or matching pairs of indicia on the two.

In one embodiment, referring to fig. 1 and 3, the liquid outlet portion f includes a tapered tube portion f1 and a port tube portion f2, the main body of the liquid collecting tube 40 is a tubular structure formed by axially extending the tapered bottom end of the tapered tube portion f1, and the port tube portion f2 is a tubular structure formed by axially extending the tapered top end of the tapered tube portion f 1; one-way seal 10 closes the end of port tube portion f2 distal from conical tube portion f 1. Therefore, the tapered tube part f1 can serve as a guide, so that the liquid injected into the liquid collection tube 40 can be distributed concentratedly around the liquid outlet part f, and the pressure generated by the liquid can be concentratedly applied to the one-way sealing member 10 during the liquid sample injection process, thereby pushing the one-way sealing member 10 open. In this embodiment, the sample addition tube part b may also adopt a structural form similar to the liquid outlet part f, so that a closed space in which the one-way sealing member 10 can be disposed can be formed between the liquid collection tube 40 and the liquid sample addition tube 30.

In one embodiment, referring to fig. 1 to 4, a pressure-bearing flange 31 is disposed on an inner peripheral wall of the liquid sample adding tube 30 adjacent to the sample adding port portion b and distributed around the sample adding port portion b, and the pressure-bearing flange 31 is used for abutting against a surface edge of the one-way sealing member 10 on a side away from the liquid outlet portion f, so that when the liquid sample adding tube 30 and the liquid collecting tube 40 are coaxially inserted and assembled, the one-way sealing member 40 can be firmly clamped between the pressure-bearing flange 31 and a port of the liquid outlet portion f, thereby achieving the limiting and fixing of the one-way sealing member 40; meanwhile, structural conditions are created for dismounting, maintenance, replacement and the like of the one-way sealing element 40; in this embodiment, the pressure-bearing flange 31 is composed of a plurality of protruding structures distributed around the sample addition port part b; in another embodiment, the pressure-bearing flange 31 can also be an annular convex structure distributed around the sample addition port portion b. In addition, a limit flange 41 is arranged on the outer peripheral wall of the liquid collecting pipe 40, and the limit flange 41 is used for abutting against the port surface of the sleeve port part e; since the pressure-bearing flange 31 and the one-way sealing element 10 are located inside the liquid collecting container, which is generally invisible or poor in visual effect, by matching the relationship between the pressure-bearing flange 31 and the limit flange 41, in the process of sleeving the liquid sampling pipe 30 on the liquid collecting pipe 40, the one-way sealing element 10 can be used as a judgment basis for firmly clamping the one-way sealing element 10 between the pressure-bearing flange 31 and the liquid outlet f after the sleeving opening e abuts against the limit flange 41.

It is right to have used specific individual example above the utility model discloses expound, only be used for helping to understand the utility model discloses, not be used for the restriction the utility model discloses. To the technical field of the utility model technical personnel, the foundation the utility model discloses an idea can also be made a plurality of simple deductions, warp or replacement.

Claims (8)

1. A liquid transfer device, comprising:

a liquid collection container having a liquid injection port portion (a) and a sample injection port portion (b), the liquid injection port portion (a) being used for injecting a liquid into the liquid collection container;

a one-way seal (10), the one-way seal (10) being disposed within an end of the liquid collection container adjacent to the sample addition port portion (b); and

the movable sealing piece (20) is inserted into the liquid collection container through the liquid injection opening part (a) and used for plugging the liquid injection opening part (a) and driving liquid to pass through the one-way sealing piece (10) and then be discharged out of the liquid collection container through the liquid injection opening part (b).

2. The liquid transfer device according to claim 1, wherein the outer peripheral wall of the sample addition port part (b) is provided with air pressure equilibrium grooves (c) which are distributed along the extending direction of the sample addition port part (b).

3. The liquid transfer device according to claim 1, wherein the one-way sealing member (10) has a sealing cover portion (11), the sealing cover portion (11) having a contour shape matching a cross-sectional shape of an end portion of the liquid collection container adjacent to the loading port portion (b);

the sealing cover part (11) is provided with a non-return slot (d), the shape of the section of the non-return slot (d) is triangular, and the notch end of the non-return slot (d) is positioned on the surface of the sealing cover part (11) facing one side of the liquid injection port part (a).

4. The liquid transfer device of claim 1, wherein the movable seal (20) comprises:

a plug part (21) for plugging the liquid injection opening part (a), wherein the plug part (21) is coaxially inserted into the liquid collection container; and

the plug rod part (22) is used for driving the plug part (21) to be close to or far away from the one-way sealing element (10) in the liquid collection container so as to drive liquid to pass through the one-way sealing element (10), and the plug rod part (22) is connected with one end, far away from the one-way sealing element (10), of the plug part (21).

5. The liquid transfer device of claim 4, wherein said movable seal (20) further comprises a flexible connecting band (23), one end of said flexible connecting band (23) being connected to said stem portion (22), and the other end of said flexible connecting band (23) being connected to a liquid collection container.

6. The liquid transfer device of any one of claims 1-5, wherein the liquid collection container comprises:

a liquid sample addition tube (30), wherein the sample addition port part (b) is formed at one end of the liquid sample addition tube (30), and a sleeve interface part (e) is formed at the other end of the liquid sample addition tube (30); and

the liquid collection pipe (40), the liquid injection port (a) is formed at one end of the liquid collection pipe (40), and the liquid outlet (f) is formed at the other end of the liquid collection pipe (40);

the liquid collecting pipe (40) is connected with the liquid sample adding pipe (30) through a sleeve interface part (e) in an inserting and sleeving way, so that the liquid outlet part (f) is positioned in the liquid sample adding pipe (30) and is coaxially opposite to the sample adding part (b); the one-way sealing element (10) is positioned in the liquid sample adding pipe (30) and clamped between the sample adding opening part (b) and the liquid outlet part (f), and the movable sealing element (20) is inserted in the liquid collecting pipe (40).

7. The liquid transfer apparatus as claimed in claim 6, wherein said outlet port portion (f) comprises a tapered tube portion (f1) and a port tube portion (f2), the main body of said liquid collection tube (40) is a tubular structure formed by axially extending the tapered bottom end of the tapered tube portion (f1), and said port tube portion (f2) is a tubular structure formed by axially extending the tapered top end of the tapered tube portion (f 1); the one-way seal (10) covers an end of the port tube portion (f2) remote from the tapered tube portion (f 1).

8. The liquid transfer device according to claim 6, wherein the inner peripheral wall of the liquid sample application tube (30) adjacent to the sample application port part (b) is provided with a pressure-bearing flange (31) distributed around the sample application port part (b), and the pressure-bearing flange (31) is used for abutting against the one-way sealing member (10); the periphery wall of the liquid collecting pipe (40) is provided with a limiting flange (41), and the limiting flange (41) is used for abutting against the port surface of the sleeve connecting part (e).

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