CN216688114U - Anti-pollution transfer device - Google Patents
Anti-pollution transfer device Download PDFInfo
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- CN216688114U CN216688114U CN202122937729.XU CN202122937729U CN216688114U CN 216688114 U CN216688114 U CN 216688114U CN 202122937729 U CN202122937729 U CN 202122937729U CN 216688114 U CN216688114 U CN 216688114U
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Abstract
The present application is directed to an anti-contamination transfer device. The device includes: the culture bottle, the hook sealing gasket, the two-way cover, the gas filter and the hose connector; the hook part of the hook sealing gasket is tightly clamped with the bottle mouth wall of the culture bottle; the main body part is provided with a first through hole and a second through hole, when the two-way cover is locked on the bottle mouth, the sealing cover inner gasket of the two-way cover is abutted with the hook sealing gasket, the first port of the first channel of the two-way cover penetrates through the first through hole, and the first channel port of the second channel of the two-way cover penetrates through the second through hole; a first silicone tube is connected between the gas outlet end of the gas filter and the second port of the first channel; a second silicone tube is connected between the connecting end of the hose joint and a second channel opening of the second channel; the first passage opening is connected with a third silicone tube. The scheme that this application provided can adopt the malleation mode to shift cell culture liquid, protects the cell sample in the cell culture liquid, reduces the pollution to cell culture liquid among the transfer process.
Description
Technical Field
The application relates to the field of biotechnology, especially, relate to anti-pollution transfer device.
Background
In the process of biological cell culture, generally, a bottle cap of a culture bottle needs to be opened to pour out a culture solution to finish biological cell sampling, or biological cells in the culture bottle are transferred to a cell factory or a fermentation tank to be amplified and cultured, and in the process of opening the bottle cap to pour out, exogenous substances are easily introduced into the culture solution, so that a pollution risk is generated.
In the prior art, in a patent with publication number CN213142009U (a multifunctional liquid transfer cap for shake flask), it is proposed that a check valve is added at the front end of a sampling head of the multifunctional liquid transfer cap for shake flask, so that sampling is safer, and pollution caused by liquid backflow in the sampling process is prevented; the pipe line area needle formula filter has solved the remaining condition of feed supplement in-process pipeline liquid, during the feed liquor, need not to use the peristaltic pump, can utilize the needle formula filter to bleed, carries out the cell sap transfer through the siphon principle.
The above prior art has the following disadvantages:
the scheme adopts suspension type gravity to transfer liquid, and the liquid is easy to contact with the bottle cap to cause pollution in the liquid transfer process, so that the problem of culture bottle sealing needs to be solved to transfer cell culture liquid in a positive pressure mode.
SUMMERY OF THE UTILITY MODEL
For overcoming the problem that exists among the correlation technique, this application provides an anti-pollution transfer device, this anti-pollution transfer device can promote the leakproofness of blake bottle, can adopt the malleation mode to shift cell culture liquid, protects the cell sample in the cell culture liquid, reduces the pollution of transfer process in to cell culture liquid.
The application provides an anti-pollution transfer device, includes:
a culture bottle 1, a hook sealing gasket 2, a two-way cover 3, a gas filter 4 and a hose connector 5;
the hook sealing gasket 2 comprises a main body part 21 and a hook part 22, and the hook part 22 is arranged at the edge of the main body part 21;
when the main body part 21 is arranged at the bottleneck 11 of the culture bottle 1, the hook part 22 is tightly clamped with the bottleneck wall 111 of the culture bottle 1, so that the hook sealing gasket 2 can be fixed at the opening of the bottleneck 11;
the main body part 21 is provided with a first through hole and a second through hole, when the two-way cover 3 is locked on the bottle mouth 11, the sealing cover inner gasket 33 of the two-way cover 3 is abutted with the hook sealing gasket 2, the first port 311 of the first channel 31 of the two-way cover 3 penetrates through the first through hole, the first channel port 321 of the second channel 32 of the two-way cover 3 penetrates through the second through hole, the diameter of the first through hole is smaller than the outer diameter of the first channel 31, and the diameter of the second through hole is smaller than the outer diameter of the second channel 32;
the gas filter 4 comprises a gas inlet 41 and a gas outlet 42, and a first silicone tube 61 is connected between the gas outlet 42 and the second port 312 of the first channel 31;
the hose joint 5 comprises a joint end 51 and a connecting end 52, and a second silicone tube 62 is connected between a second passage opening 322 of the second passage 32 and the connecting end 52;
the third silicone tube 63 is connected to the first channel port 321, and the third silicone tube 63 is used for guiding the cell culture fluid to flow along the direction from the third silicone tube 63 to the second silicone tube 62 when the gas is input into the culture flask 1 from the first port 311.
In one embodiment, a spigot 511 is provided at the connector end 51.
In one embodiment, a spiral thread 521 is provided at the connecting end 52, and the spiral thread 521 is used for preventing the second silicone tube 62 from sliding off the hose connector 5.
In one embodiment, a first strap 71 is disposed at the connection between the air outlet end 42 and the first silicone tube 61.
In one embodiment, the gas outlet end 42 and the gas inlet end 41 are respectively disposed at two ends of a filter element 43 of the gas filter 4;
the outer peripheral surfaces of the air outlet end 42 and the air inlet end 41 are provided with wedge-shaped chamfers 44;
the inner diameter of the outlet end 42 decreases in the direction of the opening of the filter cartridge 43 to the outlet end 42;
the inner diameter of the inlet end 41 decreases in the direction of the opening of the filter insert 43 to the inlet end 41.
In one embodiment, the second strap 72 is provided at the connection of the second port 312 to the first silicone tubing 61.
In one embodiment, a third strap 73 is provided at the junction of the second opening 322 and the second silicone tube 62.
In one embodiment, a fourth cable tie 74 is provided at the junction of the connecting end 52 and the second silicone tube 62.
In one embodiment, the end of the third silicone tube 63 remote from the first access port 321 contacts the bottom of the culture flask 1.
The technical scheme provided by the application can comprise the following beneficial effects:
the hook part of the hook sealing gasket is clamped with the bottle mouth wall of the culture bottle, so that the hook sealing gasket can be fixed at the opening of the bottle mouth, the problem that the position of the hook sealing gasket deviates due to the thin bottle mouth wall is avoided, the sealing performance of the culture bottle is influenced, when the two-way cover is locked at the bottle mouth, a sealing cover inner gasket of the two-way cover is abutted to the hook sealing gasket, and the sealing performance of the culture bottle is further ensured; the main body part of the hook sealing gasket is provided with a first through hole and a second through hole, when the two-way cover is locked on the bottle mouth, a first port of a first channel of the two-way cover penetrates through the first through hole, a first channel port of a second channel of the two-way cover penetrates through the second through hole, and as the diameter of the first through hole is smaller than the outer diameter of the first channel and the diameter of the second through hole is smaller than the outer diameter of the second channel, the first channel is in interference fit with the first through hole, and the second channel is in interference fit with the second through hole, so that no gap exists in the matching surface, and the sealing property of the culture bottle is ensured; a first silicone tube is connected between the air outlet end of the gas filter and the second port of the first channel, so that the cleanness of gas entering the culture bottle is improved, and the cell culture solution in the culture bottle is prevented from being polluted; the second silicone tube is connected between the second channel opening of the second channel of the hose connector and the connecting end, the third silicone tube is connected at the first channel opening, so that when gas filtered by the gas filter is input into the culture bottle from the first channel opening, the sealing performance of the culture bottle is ensured, extrusion force can be generated on cell culture solution, the third silicone tube can guide the cell culture solution to flow along the direction from the third silicone tube to the second silicone tube, and finally the cell culture solution flows out from the hose connector, so that cell samples in the cell culture solution are protected, and pollution to the cell culture solution in the transfer process is reduced.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application.
Drawings
The foregoing and other objects, features and advantages of the application will be apparent from the following more particular descriptions of exemplary embodiments of the application, as illustrated in the accompanying drawings wherein like reference numbers generally represent like parts throughout the exemplary embodiments of the application.
FIG. 1 is a schematic exploded view of a contamination transfer device according to an embodiment of the present application;
FIG. 2 is a schematic view of a portion of the structure of the anti-contamination transfer device according to the embodiment of the present application at the position of the two-way cover and the hooking sealing gasket;
FIG. 3 is a schematic view of a partial structure at a gas filter in the contamination transfer prevention device according to the embodiment of the present application;
fig. 4 is a partial schematic view of the structure of the contamination prevention transfer device at the hose joint according to the embodiment of the present application.
Detailed Description
Preferred embodiments of the present application will be described in more detail below with reference to the accompanying drawings. While the preferred embodiments of the present application are shown in the drawings, it should be understood that the present application may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used in this application and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any and all possible combinations of one or more of the associated listed items.
It should be understood that although the terms "first," "second," "third," etc. may be used herein to describe various information, these information should not be limited to these terms. These terms are only used to distinguish one type of information from another. For example, first information may also be referred to as second information, and similarly, second information may also be referred to as first information, without departing from the scope of the present application. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.
Example one
In the process of biological cell culture, generally, a bottle cap of a culture bottle needs to be opened to pour out a culture solution to finish biological cell sampling, or biological cells in the culture bottle are transferred to a cell factory or a fermentation tank to be amplified and cultured, and in the process of opening the bottle cap to pour out, exogenous substances are easily introduced into the culture solution, so that a pollution risk is generated. The prior art adopts suspension type gravity to transfer liquid, and the liquid is easy to contact with a bottle cap to cause pollution in the liquid transfer process, so the problem of culture bottle sealing needs to be solved to transfer cell culture liquid in a positive pressure mode.
To above-mentioned problem, this application embodiment provides an anti-pollution transfer device, can adopt the malleation mode to shift cell culture liquid, protects the cell sample in the cell culture liquid, reduces the pollution to cell culture liquid in the transfer process.
The technical solutions of the embodiments of the present application are described in detail below with reference to the accompanying drawings.
Referring to fig. 1 and 2, an embodiment of the contamination prevention transfer device shown in the embodiment of the present application includes:
the culture bottle 1 of the embodiment of the present application may be an injection molded thin bottleneck cell culture container, or may be other types of cell culture containers, and is not limited herein.
The hook sealing gasket 2 comprises a main body part 21 and a hook part 22, the hook part 22 is arranged at the edge of the main body part 21, it can be understood that the hook part 22 is a circle of protruding ring at the side edge of the main body part 21, and a certain gap exists between the hook part and the side edge of the main body part 21, in the embodiment of the present application, the width of the gap is smaller than the bottleneck wall 111 of the culture bottle 1, therefore, when the main body part 21 is arranged at the bottleneck 11 of the culture bottle 1, the hook part 22 is clamped with the bottleneck wall 111 of the culture bottle 1, so that the hook sealing gasket 2 can be fixed at the opening of the bottleneck 11, and the problem that the hook sealing gasket is shifted due to the thin bottleneck wall is avoided.
The main body 21 and the hook part of the hook sealing gasket 2 are integrally formed, and the material of the hook sealing gasket can be rubber or silica gel, which is determined according to the actual application situation and is not limited uniquely here. The main body 21 is provided with a first through hole and a second through hole, which correspond to the first channel 31 and the second channel 32 of the two-way cover 3, respectively. When the two-way cover 3 is locked on the bottle mouth 11, the inner sealing gasket 33 of the sealing cover of the two-way cover 3 is abutted against the hook sealing gasket 2 to further fix the hook sealing gasket 2, so that the sealing performance of the culture bottle 1 is ensured, the first port 311 of the first channel 31 of the two-way cover 3 penetrates through the first through hole, the first channel port 321 of the second channel 32 of the two-way cover 3 penetrates through the second through hole, the diameter of the first through hole is smaller than the outer diameter of the first channel 31, and the diameter of the second through hole is smaller than the outer diameter of the second channel 32.
Further, the gas filter 4 comprises a gas inlet 41 and a gas outlet 42, and a first silicone tube 61 is connected between the gas outlet 42 and the second port 312 of the first channel 31 for inputting the gas filtered by the gas filter 4 into the culture flask 1. Because the sealing inner gasket 33 and the hook sealing gasket 2 ensure the sealing performance of the culture bottle 1, after the gas is input into the culture bottle 1, the air pressure in the culture bottle 1 rises, and the extrusion force is generated on the cell culture solution.
Further, the hose connector 5 includes a connector end 51 and a connecting end 52, a second silicone tube 62 is connected between the second channel opening 322 of the second channel 32 and the connecting end 52, a third silicone tube 63 is connected to the first channel opening 321, when the air pressure in the culture bottle 1 rises and generates an extrusion force on the cell culture solution, the third silicone tube 63 guides the cell culture solution to flow along the direction from the third silicone tube 63 to the second silicone tube 62, and finally flows out from the hose connector 5, thereby completing the transfer of the cell culture solution.
The following beneficial effects can be seen from the first embodiment:
the hook part of the hook sealing gasket is tightly clamped with the wall of the mouth of the culture bottle, so that the hook sealing gasket can be fixed at the opening of the mouth, the problem that the position of the hook sealing gasket deviates due to the thin wall of the mouth of the bottle is avoided, the sealing performance of the culture bottle is influenced, and when the two-way cover is locked at the mouth of the bottle, the sealing gasket in the sealing cover of the two-way cover is abutted against the hook sealing gasket, so that the sealing performance of the culture bottle is further ensured; the main body part of the hook sealing gasket is provided with a first through hole and a second through hole, when the two-way cover is locked on the bottle mouth, a first port of a first channel of the two-way cover penetrates through the first through hole, a first channel port of a second channel of the two-way cover penetrates through the second through hole, and as the diameter of the first through hole is smaller than the outer diameter of the first channel and the diameter of the second through hole is smaller than the outer diameter of the second channel, the first channel is in interference fit with the first through hole, and the second channel is in interference fit with the second through hole, so that no gap exists in the matching surface, and the sealing property of the culture bottle is ensured; a first silicone tube is connected between the air outlet end of the gas filter and the second port of the first channel, so that the cleanness of gas entering the culture bottle is improved, and the cell culture solution in the culture bottle is prevented from being polluted; the second silicone tube is connected between the second channel opening of the second channel of the hose connector and the connecting end, the third silicone tube is connected at the first channel opening, so that when gas filtered by the gas filter is input into the culture bottle from the first channel opening, the sealing performance of the culture bottle is ensured, extrusion force can be generated on cell culture solution, the third silicone tube can guide the cell culture solution to flow along the direction from the third silicone tube to the second silicone tube, and finally the cell culture solution flows out from the hose connector, so that cell samples in the cell culture solution are protected, and pollution to the cell culture solution in the transfer process is reduced.
Example two
For ease of understanding, an embodiment of the contamination transfer device is provided below for illustration, and in practice, the gas filter 4 and hose connector 5 will be further designed to promote stability of the device of the present application.
Referring to fig. 1, 3 and 4, an embodiment of the contamination prevention transfer device shown in the embodiment of the present application includes:
the joint end 51 is provided with a pipe plug 511, in the embodiment of the present application, when the cell culture solution does not need to be transferred, the pipe plug 511 seals the joint end 51, so as to prevent external contaminants from entering the interior of the culture bottle 1. When the cell culture solution needs to be transferred, the pipe plug 511 is pulled out, and the joint end 51 is butted with the transferred target culture carrier. The material of the pipe plug 511 and the two-way cover 3 may be 316 stainless steel material, and high temperature sterilization can be performed, it is understood that in practical application, the material of the pipe plug 511 and the two-way cover 3 may be the same or different, and other materials besides the 316 stainless steel material may be used, which is determined according to practical application conditions and is not limited herein.
The connecting end 52 is provided with a spiral thread 521, and the spiral thread 521 is used for preventing the second silicone tube 62 from sliding off the hose connector 5.
The air outlet end 42 and the air inlet end 41 are respectively arranged at two ends of a filter element 43 of the gas filter 4, the peripheral surfaces of the air outlet end 42 and the air inlet end 41 are provided with wedge-shaped chamfers 44, the inner diameter of the air outlet end 42 is reduced along the direction from the filter element 43 to the opening of the air outlet end 42, and the inner diameter of the air inlet end 41 is reduced along the direction from the filter element 43 to the opening of the air inlet end 41, it can be understood that the connection opening of the first silicone tube 61 and the air outlet end 42 is sleeved in along the air outlet end 42, and the opening is gradually expanded, after the air outlet end 42 is completely sleeved, the larger the opening is expanded, the larger the tightening force is, the larger the protruding part of the wedge-shaped chamfers 44 can form a barrier on the inner wall of the first silicone tube 61, and therefore the first silicone tube 61 is prevented from slipping off the air outlet end 42.
The gas inlet 41 is used for connecting a pipeline for inputting gas, and the inputted gas may be inert gas such as nitrogen, and is determined according to practical application, and is not limited herein.
A first bandage 71 is arranged at the joint of the air outlet end 42 and the first silicone tube 61, a second bandage 72 is arranged at the joint of the second port 312 and the first silicone tube 61, a third bandage 73 is arranged at the joint of the second port 322 and the second silicone tube 62, and a fourth bandage 74 is arranged at the joint of the connecting end 52 and the second silicone tube 62. The ribbon is used for damming the silicone tube, cuts off the circulation of the cell culture fluid in the silicone tube, and is convenient for quantitatively transferring the cell culture fluid.
In order to sufficiently transfer the cell culture fluid in the culture bottle 1, the end of the third silicone tube 63 away from the first channel port 321 is in contact with the bottom of the culture bottle 1, so as to prevent the cell culture fluid from remaining on the bottom of the culture bottle 1 and causing unnecessary loss.
The aspects of the present application have been described in detail hereinabove with reference to the accompanying drawings. In the above embodiments, the descriptions of the respective embodiments have respective emphasis, and for parts that are not described in detail in a certain embodiment, reference may be made to related descriptions of other embodiments. Those skilled in the art should also appreciate that the acts and modules referred to in the specification are not necessarily required in the present application. In addition, it can be understood that the steps in the method of the embodiment of the present application may be sequentially adjusted, combined, and deleted according to actual needs, and the modules in the device of the embodiment of the present application may be combined, divided, and deleted according to actual needs.
Having described embodiments of the present application, the foregoing description is intended to be exemplary, not exhaustive, and not limited to the disclosed embodiments. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen in order to best explain the principles of the embodiments, the practical application, or improvements made to the technology in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.
Claims (9)
1. An anti-contamination transfer device, comprising:
a culture bottle (1), a hook sealing gasket (2), a two-way cover (3), a gas filter (4) and a hose connector (5);
the hook sealing gasket (2) comprises a main body part (21) and a hook part (22), and the hook part (22) is arranged at the edge of the main body part (21);
when the main body part (21) is arranged at the bottle mouth (11) of the culture bottle (1), the hook part (22) is tightly clamped with the bottle mouth wall (111) of the culture bottle (1), so that the hook sealing gasket (2) can be fixed at the opening of the bottle mouth (11);
a first through hole and a second through hole are formed in the main body part (21), when the two-way cover (3) is locked on the bottle opening (11), a sealing cover inner gasket (33) of the two-way cover (3) is abutted to the hook sealing gasket (2), a first port (311) of a first channel (31) of the two-way cover (3) penetrates through the first through hole, a first channel port (321) of a second channel (32) of the two-way cover (3) penetrates through the second through hole, the diameter of the first through hole is smaller than the outer diameter of the first channel (31), and the diameter of the second through hole is smaller than the outer diameter of the second channel (32);
the gas filter (4) comprises a gas inlet end (41) and a gas outlet end (42), and a first silicone tube (61) is connected between the gas outlet end (42) and the second port (312) of the first channel (31);
the hose joint (5) comprises a joint end (51) and a connecting end (52), and a second silicone tube (62) is connected between a second channel opening (322) of the second channel (32) and the connecting end (52);
the first channel port (321) is connected with a third silicone tube (63), and the third silicone tube (63) is used for guiding the cell culture solution to flow along the direction from the third silicone tube (63) to the second silicone tube (62) when gas is input into the culture bottle (1) from the first port (311).
2. The anti-contamination transfer device of claim 1,
and a pipe plug (511) is arranged at the joint end (51).
3. The anti-contamination transfer device of claim 1,
the connecting end (52) is provided with a spiral thread (521), and the spiral thread (521) is used for preventing the second silicone tube (62) from sliding off the hose connector (5).
4. The anti-contamination transfer device of claim 1,
and a first binding belt (71) is arranged at the joint of the air outlet end (42) and the first silicone tube (61).
5. The anti-contamination transfer device of claim 1,
the air outlet end (42) and the air inlet end (41) are respectively arranged at two ends of a filtering element (43) of the gas filter (4);
the peripheral surfaces of the air outlet end (42) and the air inlet end (41) are provided with wedge-shaped chamfers (44);
the inner diameter of the gas outlet end (42) is reduced along the direction from the filter element (43) to the opening of the gas outlet end (42);
the inner diameter of the air inlet end (41) decreases in the direction of the opening of the filter insert (43) to the air inlet end (41).
6. The anti-contamination transfer device of claim 1,
and a second bandage (72) is arranged at the joint of the second port (312) and the first silicone tube (61).
7. The anti-contamination transfer device of claim 1,
and a third bandage (73) is arranged at the joint of the second passage opening (322) and the second silicone tube (62).
8. The anti-contamination transfer device of claim 1,
and a fourth bandage (74) is arranged at the joint of the connecting end (52) and the second silicone tube (62).
9. The anti-contamination transfer device of claim 1,
one end of the third silicone tube (63) far away from the first passage port (321) is contacted with the bottom of the culture bottle (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122937729.XU CN216688114U (en) | 2021-11-27 | 2021-11-27 | Anti-pollution transfer device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122937729.XU CN216688114U (en) | 2021-11-27 | 2021-11-27 | Anti-pollution transfer device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216688114U true CN216688114U (en) | 2022-06-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122937729.XU Active CN216688114U (en) | 2021-11-27 | 2021-11-27 | Anti-pollution transfer device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216688114U (en) |
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2021
- 2021-11-27 CN CN202122937729.XU patent/CN216688114U/en active Active
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