CN213835275U - Separation device for ovarian cancer circulating tumor cell exosomes - Google Patents

Separation device for ovarian cancer circulating tumor cell exosomes Download PDF

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Publication number
CN213835275U
CN213835275U CN202022589508.3U CN202022589508U CN213835275U CN 213835275 U CN213835275 U CN 213835275U CN 202022589508 U CN202022589508 U CN 202022589508U CN 213835275 U CN213835275 U CN 213835275U
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test tube
base
motor
electric putter
installation piece
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CN202022589508.3U
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Chinese (zh)
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许永杰
罗洁
蒋伟峰
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Guizhou Provincial Peoples Hospital
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Guizhou Provincial Peoples Hospital
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Abstract

The utility model provides a separator of ovarian cancer circulating tumor cell exosome, include: base, top cap, motor and motor, surface mounting has the handle on the top cap, surface mounting has the outer wall on the base, the outer wall passes through threaded connection with the top cap, base internally mounted has the motor, surface mounting has the main shaft on the motor, surface mounting has the installation piece on the main shaft, the test-tube slot has been seted up to installation piece upper surface, install No. two electric putter on the inside lower surface of installation piece, surface mounting has the motor on the base, surface mounting has an electric putter on the base, surface mounting has the baffle on an electric putter, compared with the prior art, the utility model discloses following beneficial effect has: through increasing motor, electric putter and No. two electric putter, realized can vibrate earlier then the centrifugation in the machine, reduced manual operation to just can see the condition in the test tube in the separating centrifuge, it is more convenient to use.

Description

Separation device for ovarian cancer circulating tumor cell exosomes
Technical Field
The utility model belongs to the field of separation equipment, in particular to a separation device for ovarian cancer circulating tumor cell exosomes.
Background
At present, the traditional methods for exosome separation are differential ultracentrifugation, density gradient ultracentrifugation and the like, the density gradient ultracentrifugation needs to ultracentrifuge a sample and a gradient material together, and different components in the sample are settled to respective equal density areas.
However, in practical use, if the existing separation device has a single function, the cell solution needs to be shaken up manually to be centrifuged, so that the use is limited, and the condition in the test tube can be seen only by taking out the test tube in the separator.
Therefore, there is a need for a device for isolating circulating tumor cell exosomes from ovarian cancer.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the problems existing in the prior art, and provides a separating device for ovarian cancer circulating tumor cell exosomes, which is provided by adding a motor, an electric push rod and a second electric push rod.
The technical scheme of the utility model is realized like this: an apparatus for isolating circulating tumor cell exosomes for ovarian cancer, comprising: base, top cap, motor and motor, surface mounting has the handle on the top cap, surface mounting has the outer wall on the base, the outer wall passes through threaded connection with the top cap, base internally mounted has the motor, surface mounting has the main shaft on the motor, surface mounting has the installation piece on the main shaft, the test tube groove has been seted up to installation piece upper surface, install No. two electric putter on the inside lower surface of installation piece, surface mounting has the motor on the base, surface mounting has an electric putter on the base, an electric putter upper surface mounting has the baffle, the test tube hole has been seted up to baffle upper surface and lower surface, surface mounting has the supporting shoe on the base, the spout has been seted up on the circumference surface of installation piece.
As a preferred embodiment, the spindle penetrates through the upper surface of the base, two motors are mounted on the upper surface of the base, the two motors are respectively mounted on two sides of the spindle, and the height of the two motors is equal to the height of a gap between the base and the mounting block.
As a preferred embodiment, a plurality of second electric push rods are installed inside the installation block, a plurality of test tube slots are formed in the upper surface of the installation block, the number of the second electric push rods is equal to that of the test tube slots, and each test tube slot is installed on the upper surface of the second electric push rod.
As a preferred embodiment, two supporting blocks are installed on the upper surface of the base, the two supporting blocks are respectively installed at two ends of the installation block, and the tops of the supporting blocks are installed in the sliding grooves.
As a preferred embodiment, two first electric push rods are mounted on the upper surface of the base, the two first electric push rods are respectively mounted at two ends of the mounting block, a plurality of test tube holes are formed in the upper surface and the lower surface of the baffle, the number of the test tube holes is equal to the number of the test tube grooves, and each test tube hole is located right above the corresponding test tube groove.
After the technical scheme is adopted, the beneficial effects of the utility model are that: through increasing motor, electric putter and No. two electric putter, realized can vibrate earlier then the centrifugation in the machine, reduced manual operation to just can see the condition in the test tube in the separating centrifuge, it is more convenient to use.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive exercise.
Fig. 1 is a schematic diagram of the overall structure of the device for separating the exosomes from the circulating tumor cells of ovarian cancer of the present invention.
Fig. 2 is a schematic diagram of a baffle and an electric push rod of the device for separating the ovarian cancer circulating tumor cell exosomes of the present invention.
Fig. 3 is a schematic view of the installation block of the device for separating the exosomes from the circulating tumor cells of ovarian cancer of the present invention.
In the figure, 1-handle, 2-top cover, 3-test tube hole, 4-outer wall, 5-base, 6-motor, 7-main shaft, 8-first electric push rod, 9-motor, 10-second electric push rod, 11-supporting block, 12-chute, 13-mounting block, 14-test tube groove, 15-baffle.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
Referring to fig. 1 to 3, the present invention provides a technical solution: an apparatus for isolating circulating tumor cell exosomes for ovarian cancer, comprising: base 5, top cap 2, motor 6 and motor 9, surface mounting has handle 1 on the top cap 2, surface mounting has outer wall 4 on the base 5, outer wall 4 passes through threaded connection with top cap 2, 5 internally mounted of base has motor 6, surface mounting has main shaft 7 on the motor 6, surface mounting has installation piece 13 on the main shaft 7, test tube groove 14 has been seted up to installation piece 13 upper surface, install No. two electric putter 10 on the inside lower surface of installation piece 13, surface mounting has motor 9 on the base 5, 5 upper surface mounting of base has electric putter 8, 8 upper surface mounting of electric putter has baffle 15, baffle 15 upper surface and lower surface have seted up test tube hole 3, 5 upper surface mounting of base has supporting shoe 11, spout 12 has been seted up on the surface to the circumference of installation piece 13.
The main shaft 7 runs through the upper surface of the base 5, the two motors 9 are mounted on the upper surface of the base 5, the two motors 9 are respectively mounted on two sides of the main shaft 7, the heights of the two motors 9 are equal to the height of a gap between the base 5 and the mounting block 13, the motors 9 are micro vibrating motors 9 and can provide vibration for the mounting block 13, the outer wall 4 is made of glass, the transparency of the glass is high, and the condition of the inside can be directly observed outside the separator.
Installation piece 13 internally mounted has a plurality of electric putter 10 No. two, and a plurality of test tube grooves 14 have been seted up to installation piece 13 upper surface, and the quantity of No. two electric putter 10 equals with the quantity of test tube grooves 14, and every test tube groove 14 all installs at No. two electric putter 10 upper surfaces, and the diameter of No. two electric putter 10 equals with the diameter of test tube grooves 14, and No. two electric putter 10 just can follow the test tube upwards-pushing of test tube grooves 14 bottom like this.
Two supporting blocks 11 are installed on the upper surface of the base 5, the two supporting blocks 11 are installed at two ends of an installation block 13 respectively, and the tops of the supporting blocks 11 are installed in the sliding grooves 12.
5 upper surface mounting of base has two electric putter 8 No. one, and two electric putter 8 are installed respectively at installation piece 13 both ends, and a plurality of test tube holes 3 have been seted up to the upper surface and the lower surface of baffle 15, and the quantity of test tube hole 3 equals with the quantity of test tube groove 14, and every test tube hole 3 all is directly over the test tube groove 14 that corresponds.
As an embodiment of the present invention: firstly fixing the base 5, circularly rotating the top cover 2, then lifting the handle 1 to take the top cover 2 off the outer wall 4, putting the test tube filled with the ovarian cancer circulating tumor cell exosome into the test tube groove 14, then the coping 2 is covered and rotated to fix the coping 2 well, because the ovarian cancer circulating tumor cell exosome needs to be shaken up first to be centrifuged, therefore, the motor 9 is turned on first, the motor 9 vibrates, the vibration is transmitted to the test tube through the mounting block 13, the ovarian cancer circulating tumor cell exosome vibrates uniformly, the motor 6 is turned on again, the spindle in the motor 6 drives the spindle 7 to rotate, the spindle 7 drives the mounting block 13 to rotate, the mounting block 13 rotates to drive the test tube in the test tube groove 14 to rotate, the ovarian cancer circulating tumor cell exosome is centrifuged, the centrifugation of the ovarian cancer circulating tumor cell exosome is completed after a period of time, and the motor 6 is turned off.
In order to ensure the condition in the test tube, the first electric push rod 8 and the second electric push rod 10 are firstly opened, at the moment, the second electric push rod 10 extends to eject the test tube out of the test tube groove 14, the first electric push rod 8 extends to raise the height of the baffle plate 15, the raised height of the baffle plate 15 is equal to the height of the test tube which is ejected, the test tube is ensured to incline because no mounting block 13 ensures balance when being ejected, the test tube always penetrates through the test tube hole 3, at the moment, the working personnel can see the separated appearance of the ovarian cancer circulating tumor cell exosome in the test tube through the outer wall 4 made of the glass material outside the centrifuge, if the separation is required to be continuously carried out, the first electric push rod 8 and the second electric push rod 10 are shortened and then the motor 6 is continuously turned on, if the test tube does not need to be separated continuously, the top cover 2 is opened, and the first electric push rod 8 and the second electric push rod 10 are shortened after the test tube is taken out.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (5)

1. An apparatus for isolating circulating tumor cell exosomes for ovarian cancer, comprising: base, top cap, motor and motor, its characterized in that: the utility model discloses a test tube test device, including top cap, base upper surface, outer wall, base inner mounting has the motor, surface mounting has the main shaft on the motor, surface mounting has the installation piece on the main shaft, the test tube groove has been seted up to installation piece upper surface, install No. two electric putter on the inside lower surface of installation piece, surface mounting has the motor on the base, surface mounting has an electric putter on the base, surface mounting has the baffle on the electric putter, the test tube hole has been seted up to baffle upper surface and lower surface, surface mounting has the supporting shoe on the base, the spout has been seted up on the circumference surface of installation piece.
2. The device of claim 1, wherein said device comprises: the main shaft runs through the base upper surface, base upper surface mounting has two motors, two the motor is installed respectively in the both sides of main shaft, two the height of motor equals with the clearance height between base and the installation piece.
3. The device of claim 1, wherein said device comprises: the utility model discloses a test tube test device, including installation piece, mounting piece upper surface, a plurality of electric putter are installed to installation piece internally mounted, a plurality of test tube grooves have been seted up to installation piece upper surface, the quantity of No. two electric putter equals with the quantity in test tube groove, every the test tube groove all installs No. two electric putter upper surface.
4. The device of claim 1, wherein said device comprises: the upper surface of the base is provided with two supporting blocks, the two supporting blocks are respectively arranged at two ends of the mounting block, and the tops of the supporting blocks are arranged in the sliding grooves.
5. The device of claim 1, wherein said device comprises: the base upper surface mounting has two electric putter one, two electric putter installs respectively at installation piece both ends, a plurality of test tube holes have been seted up to the upper surface and the lower surface of baffle, the quantity of test tube hole equals with the quantity of test tube groove, every the test tube hole all is directly over the test tube groove that corresponds.
CN202022589508.3U 2020-11-11 2020-11-11 Separation device for ovarian cancer circulating tumor cell exosomes Active CN213835275U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202022589508.3U CN213835275U (en) 2020-11-11 2020-11-11 Separation device for ovarian cancer circulating tumor cell exosomes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202022589508.3U CN213835275U (en) 2020-11-11 2020-11-11 Separation device for ovarian cancer circulating tumor cell exosomes

Publications (1)

Publication Number Publication Date
CN213835275U true CN213835275U (en) 2021-07-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202022589508.3U Active CN213835275U (en) 2020-11-11 2020-11-11 Separation device for ovarian cancer circulating tumor cell exosomes

Country Status (1)

Country Link
CN (1) CN213835275U (en)

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