CN213823555U - Protein purification chromatographic column - Google Patents
Protein purification chromatographic column Download PDFInfo
- Publication number
- CN213823555U CN213823555U CN202022670783.8U CN202022670783U CN213823555U CN 213823555 U CN213823555 U CN 213823555U CN 202022670783 U CN202022670783 U CN 202022670783U CN 213823555 U CN213823555 U CN 213823555U
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- Prior art keywords
- fixing frame
- cylinder
- lower fixing
- upper fixing
- column
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- 238000001742 protein purification Methods 0.000 title claims abstract description 21
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 23
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 11
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 238000007789 sealing Methods 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 3
- 238000000746 purification Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000001042 affinity chromatography Methods 0.000 description 3
- 239000012535 impurity Substances 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000012460 protein solution Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
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- Peptides Or Proteins (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The utility model discloses a protein purification chromatography column, which comprises a support frame and a column body, wherein the support frame comprises a base, a cylinder, an upper fixing frame and a lower fixing frame, the cylinder is arranged on the base, the upper fixing frame and the lower fixing frame are respectively connected on the cylinder in a sliding way, the upper fixing frame is positioned above the lower fixing frame, and the upper fixing frame and the lower fixing frame are used for clamping the column body; the top of the column body is provided with a top cap, and the top cap is provided with a rubber connector for adding protein liquid into the column body; the bottom of the column body is provided with a funnel, the outlet at the bottom of the funnel is provided with a leakage pipe, and a piston head is arranged between the leakage pipe and the funnel. The protein purification chromatographic column is simple in structure by arranging the separable cylinder, the upper fixing frame and the lower fixing frame, and the upper fixing frame, the lower fixing frame and the cylinder are conveniently separated, so that the protein purification chromatographic column is convenient to store when not used, occupies a small space and is convenient to transport.
Description
Technical Field
The utility model belongs to the technical field of the protein purification apparatus, in particular to protein purification chromatographic column.
Background
The isolation and purification of proteins is a widely used technique in biochemical research applications, and a typical eukaryotic cell may contain thousands of different proteins, some very abundant, some only in a few copies, and in order to study a certain protein, it is necessary to first purify the protein from other proteins and non-protein molecules.
The chromatographic column is a main body in the gel chromatography technology, and has different distribution coefficients in a stationary phase and a mobile phase according to components in a sample mixture, so that separation is performed.
SUMMERY OF THE UTILITY MODEL
The main purpose of the utility model is to provide a protein purification chromatographic column, which is convenient for disassembly and assembly, occupies small space, and is convenient for transportation and storage.
The utility model discloses a secondary purpose lies in providing a protein purification chromatographic column, and sealing performance is good, and the adsorption object thing effect is excellent, and purification chromatography is efficient.
In order to achieve the above purpose, the solution of the present invention is:
a protein purification chromatography column comprises a support frame and a column body, wherein the support frame comprises a base, a cylinder, an upper fixing frame and a lower fixing frame, the cylinder is arranged on the base, the upper fixing frame and the lower fixing frame are respectively connected to the cylinder in a sliding mode, the upper fixing frame is located above the lower fixing frame, and the upper fixing frame and the lower fixing frame are used for clamping the column body; the top of the column body is provided with a top cap, and the top cap is provided with a rubber connector for adding protein liquid into the column body; the bottom of the column body is provided with a funnel, the outlet at the bottom of the funnel is provided with a leakage pipe, and a piston head is arranged between the leakage pipe and the funnel.
Above-mentioned upper bracket and lower mount all are equipped with first knob in the junction with the cylinder, through adjusting first knob, can make upper bracket, lower mount and cylindrical contact fastening or relax.
The contact parts of the first rotating button and the upper fixing frame and the lower fixing frame are correspondingly provided with thread structures.
The upper fixing frame and the lower fixing frame are provided with second rotating buttons at the positions for clamping the cylinder, and the clamping force of the upper fixing frame and the lower fixing frame on the cylinder can be fastened or loosened by adjusting the second rotating buttons.
The contact parts of the second rotating button and the upper fixing frame and the lower fixing frame are correspondingly provided with thread structures.
The cylinder is a cylinder, the outer surface of the cylinder is provided with capacity marking scale marks, and the cylinder is made of organic glass materials.
And sealing rubber is arranged between the top cap and the column body.
A first hydrophilic sieve plate and a second hydrophilic sieve plate are sequentially arranged in the column from bottom to top, and the pore diameter of the first hydrophilic sieve plate and the pore diameter of the second hydrophilic sieve plate are 0.10-0.45 mu m.
And the bottom end of the base is fixedly provided with an anti-slip column.
After the scheme is adopted, the utility model discloses compare prior art's beneficial effect is:
(1) the utility model has simple structure by arranging the separable cylinder, the upper fixing frame and the lower fixing frame, and the upper fixing frame, the lower fixing frame and the cylinder are conveniently separated, thereby being convenient for storage when not in use, occupying small space and being convenient for transportation;
(2) the utility model can effectively increase the purification and chromatography efficiency by arranging the sealing rubber;
(3) the utility model discloses a set up first hydrophilicity sieve and second hydrophilicity sieve, can improve the absorption target effect in the chromatographic column, improve purification chromatography efficiency.
Drawings
Fig. 1 is a schematic perspective view of the present invention;
fig. 2 is a cross-sectional view of the present invention.
Reference numerals:
1. a support frame; 2. a cylinder; 3. a base; 4. a cylinder;
5. an upper fixing frame; 6. a lower fixing frame;
7. a first rotary knob; 8. a second rotary knob;
9. a top cap; 10. a rubber connector; 11. a funnel;
12. a piston head; 13. a leak pipe;
14. a first hydrophilic screen deck; 15. a second hydrophilic screen deck;
16. and (4) anti-slip columns.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.
As shown in fig. 1 and 2, the utility model provides a protein purification chromatography column, which comprises a support frame 1 and a column body 2, wherein the support frame 1 is composed of a base 3, a column 4, an upper fixing frame 5 and a lower fixing frame 6, the top end of the base 3 is fixedly provided with the column 4, one side of the column 4 is provided with the upper fixing frame 5 and the lower fixing frame 6, one sides of the upper fixing frame 5 and the lower fixing frame 6 are both fixedly provided with a first rotating button 7, and the other sides of the upper fixing frame 5 and the lower fixing frame 6 are fixedly provided with a second rotating button 8; fixed mounting has cylinder 2 between upper mounting frame 5 and the lower mounting frame 6, and 2 top fixed mounting of cylinder have hood 9, and the hood 9 top is provided with rubber connector 10, and 2 bottom fixed mounting of cylinder have funnel 11, and 11 bottom fixed mounting of funnel have piston head 12, and 12 bottom fixed mounting of piston head have a small flow pipe 13.
As a preferred embodiment of the present invention, as shown in fig. 1, the cylinder 4 is fixedly installed by an upper fixing frame 5 and a lower fixing frame 6, the cylinder 4 penetrates one side of the upper fixing frame 5 and one side of the lower fixing frame 6, and the cylinder 4 is slidably connected with the upper fixing frame 5 and the lower fixing frame 6 respectively; through setting up the upper bracket and fixing down, can make the cylinder fix on upper bracket and fixing down, simple structure, easy to assemble and dismantlement.
As a preferred embodiment of the present invention, as shown in fig. 1, two sets of first and second rotary knobs 7 and 8 are provided, the contact portions between the first and second rotary knobs 7 and 8 and the upper and lower fixing frames 5 and 6 are provided with screw structures, and the surfaces of the first and second rotary knobs 7 and 8 are provided with anti-skid threads; through setting up first knob and second knob, fix the upper fixing frame and lower mount on the cylinder.
As a preferred embodiment of the present invention, as shown in fig. 1, the cylinder 2 is a cylinder, the outer surface of the cylinder 2 is provided with volume mark scale marks, and the cylinder 2 is made of organic glass material; through setting up capacity mark scale mark, conveniently observe the internal capacity of column.
As a preferred embodiment of the present invention, as shown in fig. 1, a sealing rubber is disposed between the top cap 9 and the column body 2, a rubber connector 10 is fixedly mounted on the top end of the top cap 9, the rubber connectors 10 are provided in a plurality of groups, and the rubber connector 10 penetrates through the top end of the top cap 9 and is connected into the column body 2; by arranging the sealing rubber, the purification and chromatography efficiency can be improved.
As a preferred embodiment of the present invention, as shown in fig. 2, a first hydrophilic sieve plate 14 and a second hydrophilic sieve plate 15 are disposed in the column body 2, and the aperture of the first hydrophilic sieve plate 14 and the aperture of the second hydrophilic sieve plate 15 are 0.10 to 0.45 μm; through setting up first hydrophilicity sieve and second hydrophilicity sieve, can improve the absorption target effect in the chromatographic column, improve purification chromatography efficiency.
As a preferred embodiment of the present invention, as shown in fig. 1, the bottom end of the base 3 is fixedly installed with the anti-skid columns 16, the anti-skid columns 16 are provided with four groups, and the anti-skid columns 16 are fixedly installed at four corners of the base 3; through setting up the non-slip column, can make the support frame steadily place.
The utility model discloses a theory of operation is: when the device works, the top cap 9 is opened, the first hydrophilic sieve plate 14 and the second hydrophilic sieve plate 15 are fixedly arranged in the column body 2, and through the arrangement of the double-layer sieve plate for filtration, impurities in a protein solution can be prevented from blocking the affinity chromatography column, so that the effect of adsorbing a target in the chromatography column can be improved; then, filling the column body 2, covering a top cap 9, arranging a first rotating button 7 and a second rotating button 8, adjusting the first rotating button 7 to fix the upper fixing frame 5 and the lower fixing frame 6 on the support frame 1 and adjust the upper fixing frame and the lower fixing frame to proper positions, adjusting the second rotating button 8 to fixedly install the column body 2 between the upper fixing frame 5 and the lower fixing frame 6, adding protein liquid into the column body 2 through a rubber connector 10 at the top end of the top cap 9, carrying out affinity chromatography on the protein liquid, and rotating a piston head 12 to enable the chromatography liquid to flow out of a leakage pipe 13 through a funnel 11; according to the protein purification chromatography column, the volume mark scale marks are arranged, so that the volume in the column body can be conveniently observed, and the purification chromatography efficiency can be effectively improved by arranging the sealing rubber; through setting up first hydrophilicity sieve and second hydrophilicity sieve, can prevent that the impurity in the protein solution from blockking affinity chromatography post, can effectively solve current chromatography post when carrying out sample separation purification, the leakproofness is not good, and the absorption target object effect in the chromatography post is poor, and the problem that purification chromatography is inefficient has higher practical value.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description of the present invention and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above should not be understood to necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples described in this specification can be combined and combined by those skilled in the art.
Although embodiments of the present invention have been shown and described, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.
Claims (9)
1. A protein purification chromatographic column is characterized in that: the device comprises a support frame and a cylinder body, wherein the support frame comprises a base, a cylinder, an upper fixing frame and a lower fixing frame, the cylinder is arranged on the base, the upper fixing frame and the lower fixing frame are respectively connected to the cylinder in a sliding manner, the upper fixing frame is positioned above the lower fixing frame, and the upper fixing frame and the lower fixing frame are used for clamping the cylinder body; the top of the column body is provided with a top cap, and the top cap is provided with a rubber connector for adding protein liquid into the column body; the bottom of the column body is provided with a funnel, the outlet at the bottom of the funnel is provided with a leakage pipe, and a piston head is arranged between the leakage pipe and the funnel.
2. The protein purification chromatography column of claim 1, wherein: the upper fixing frame and the lower fixing frame are provided with first rotating buttons at the connecting parts of the upper fixing frame and the lower fixing frame and the cylinder, and the upper fixing frame, the lower fixing frame and the cylinder can be tightly or loosely contacted by adjusting the first rotating buttons.
3. A protein purification chromatography column as claimed in claim 2, wherein: and the contact parts of the first rotating button and the upper fixing frame and the lower fixing frame are correspondingly provided with thread structures.
4. The protein purification chromatography column of claim 1, wherein: the upper fixing frame and the lower fixing frame are provided with second rotating buttons at positions for clamping the cylinder, and the clamping force of the upper fixing frame and the lower fixing frame on the cylinder can be fastened or loosened by adjusting the second rotating buttons.
5. The protein purification chromatography column of claim 4, wherein: and the contact parts of the second rotating button and the upper fixing frame and the lower fixing frame are correspondingly provided with thread structures.
6. The protein purification chromatography column of claim 1, wherein: the cylinder is the cylinder, and the cylinder surface is provided with the capacity mark scale mark, and the cylinder is made by organic glass material.
7. The protein purification chromatography column of claim 1, wherein: and sealing rubber is arranged between the top cap and the column body.
8. The protein purification chromatography column of claim 1, wherein: a first hydrophilic sieve plate and a second hydrophilic sieve plate are sequentially arranged in the column body from bottom to top, and the aperture of each of the first hydrophilic sieve plate and the second hydrophilic sieve plate is 0.10-0.45 mu m.
9. The protein purification chromatography column of claim 1, wherein: and the bottom end of the base is fixedly provided with an anti-slip column.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022670783.8U CN213823555U (en) | 2020-11-18 | 2020-11-18 | Protein purification chromatographic column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022670783.8U CN213823555U (en) | 2020-11-18 | 2020-11-18 | Protein purification chromatographic column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213823555U true CN213823555U (en) | 2021-07-30 |
Family
ID=77017399
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022670783.8U Expired - Fee Related CN213823555U (en) | 2020-11-18 | 2020-11-18 | Protein purification chromatographic column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213823555U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114539350A (en) * | 2022-03-23 | 2022-05-27 | 长睿生物技术(成都)有限公司 | Protein purification device |
-
2020
- 2020-11-18 CN CN202022670783.8U patent/CN213823555U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114539350A (en) * | 2022-03-23 | 2022-05-27 | 长睿生物技术(成都)有限公司 | Protein purification device |
| CN114539350B (en) * | 2022-03-23 | 2024-03-26 | 长睿生物技术(成都)有限公司 | Protein purification device |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210730 |