CN218036624U - Liquid chromatography column joint - Google Patents
Liquid chromatography column joint Download PDFInfo
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- CN218036624U CN218036624U CN202221990806.6U CN202221990806U CN218036624U CN 218036624 U CN218036624 U CN 218036624U CN 202221990806 U CN202221990806 U CN 202221990806U CN 218036624 U CN218036624 U CN 218036624U
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Abstract
The utility model discloses a liquid chromatography column connects belongs to liquid chromatography accessory technical field. A liquid chromatography column joint comprises a chromatography column body, wherein mounting seats are arranged on two sides of the chromatography column body, a cylinder is fixedly connected to the side wall of each mounting seat, four sliding rods are connected in the cylinder in a sliding mode and distributed in a circumferential mode, a clamping block is fixedly connected to the tops of the sliding rods and is in an arc shape, four sliding grooves are formed in the cylinder in a circumferential mode, sliding plates are connected in the sliding grooves in a sliding mode and are fixedly connected to the tops of the sliding plates, springs are fixedly connected to the ends, away from the sliding rods, of the sliding plates, and the ends, away from the sliding plates, of the springs are fixedly connected to the bottoms of the sliding grooves; the utility model discloses can be fit for installing on the liquid chromatography post of not unidimensional size according to the distance of the size of a dimension regulation fixture block joint of different chromatographic column bodies.
Description
Technical Field
The utility model relates to a liquid chromatogram accessory technical field especially relates to a liquid chromatogram column joint.
Background
The high performance liquid chromatograph is one of the most commonly used instruments in pharmaceutical inspection, and is commonly used for content determination of medicines, related substance inspection, determination of genotoxic impurities, compatibility experiments and the like.
High performance liquid chromatography is classified into liquid-solid adsorption chromatography, liquid-liquid partition chromatography (normal phase and reverse phase), ion exchange chromatography, ion-pair chromatography, and size exclusion chromatography according to the difference in separation mechanism. The separation and preparation of the compounds by high performance liquid chromatography are carried out in a liquid chromatography column. According to the property of the substance to be separated, a corresponding liquid chromatographic column is required to be selected to realize the separation or preparation.
The liquid chromatographic columns are classified according to chromatographic stationary phase matrixes, and the following are mainly adopted:
1. silica gel substrate:
1.1 reverse phase chromatography column:
the reverse phase chromatographic packing is usually based on silica gel with a bonding phase having relatively less polar functional groups bonded to the surface. The mobile phase used in reverse phase chromatography is relatively polar and is usually water, a mixture of buffer and methanol, hexacyanoferrate, etc. The order in which the sample flows out of the column is such that the more polar groups are washed out first, while the less polar components are more strongly retained on the column. As the reverse phase filler, C18 (ODS), C8 (MOS), C4 (B), C6H5 (Phenyl) and the like are generally used.
1.2 normal phase chromatography:
the stationary phases for normal phase chromatography are typically Silica gel (Silica), and other bound phase fillers with polar functional groups, such as amine groups (NH 2, APS) and cyano groups (CN, CPS). Because the silicon hydroxyl (SiOH) or other groups on the surface of silica gel are highly polar, the separation order depends on the polarity of each component in the sample, i.e., the components with strong and weak polarity are washed out of the chromatographic column first. The polarity of the mobile phase used in normal phase chromatography is lower than that of the stationary phase, such as: n-ethane (Hexane), chloroform (Chloroform), dichloromethane (Methylene Chloride), and the like.
1.3 ion exchange chromatography column:
the method comprises the following steps of (1) bonding a sulfonated cross-linked strong anion/cation silica gel chromatographic column by a common specification: strong anion chromatography column (SAX), strong cation exchange chromatography column (SCX)
2. Polymer matrix:
the polymer flavors are mostly polystyrene-divinylbenzene or polymethylpropionate, etc., and have a major advantage in that they can be used at a pH of 1 to 14. Such fillers are more hydrophobic than the C18 fillers of the silica gel matrix; the macroporous polymer filler is very effective for separating protein and other samples. The existing polymer packing has the disadvantage of lower column efficiency of chromatographic columns compared with silica gel matrix packing.
All polymer matrices will expand or contract when the mobile phase changes. High cross-linking polymer fillers for HPLC have limited expansion and contraction. Solvents or small molecules tend to penetrate into the polymer matrix, causing the small molecules to be less column efficient in such matrices because the mass transfer of the small molecules is slower in the polymer matrix than in the ceramic matrix. For macromolecules like proteins or synthetic polymers, the performance of the polymer matrix is comparable to that of the ceramic matrix. Therefore, polymer matrices are widely used for the separation of macromolecular substances.
3. Other inorganic fillers:
other inorganic packing chromatographic columns for HPLC have also been commercialized. Due to their special properties, they are generally limited to specific applications. Such as graphitized carbon, are also being used as reversed phase chromatography packing materials. The separation of this filler is different from that of the alkyl-bonded phase of the silica matrix, the surface of the graphitized carbon is the basis for retention, no other surface modification is needed, the column filler generally has stronger retention than that of the alkyl-bonded silica or porous polymer filler, the graphitized carbon can be used for separating certain geometric structures, and the column can be used at any pH and temperature because the HPLC mobile phase can not be dissolved. Alumina can also be used in HPLC, and its particles have high rigidity and can be made into stable column bed, and its advantage is that it can be used in mobile phase with pH up to 12. However, since alumina has a strong effect with alkaline compounds and the application range is limited to a certain extent, it is not widely used, and the new zirconia filler can also be used in HPLC, commercial porous zirconia microsphere chromatographic column with only polymer coating, the application pH range is 1-14, and the temperature can reach 100 ℃.
For the chromatographic column with the same matrix, the length and the width are divided into the following parts:
short column (column inner diameter 15-100 mm), short running time, low column pressure;
long column (column inner diameter 150-250 mm), high resolution and long operation time;
the narrow-diameter column (the inner diameter of the column is less than 2.1 mm) has high detection sensitivity;
the wide-diameter column (the inner diameter of the column is 3-7.8 mm) has high sample loading capacity and is pollution-resistant;
preparation of column (> 10 mm internal column diameter) -for purification preparation.
Therefore, the types of the chromatographic columns are various, and in the actual detection work, the corresponding liquid chromatographic columns are often replaced according to the needs of the substances to be separated.
However, most of the existing chromatographic column joints are connected to the liquid chromatographic column through threads, and the existing chromatographic column joints have limitations and cannot be applied to liquid chromatographic columns with different sizes.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the chromatographic column among the prior art and connecting mostly through threaded connection on the liquid chromatographic column, have the limitation, can not be applicable to the problem of the liquid chromatographic column of different sizes, and a liquid chromatographic column who proposes connects.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the utility model provides a liquid chromatography column connects, includes the chromatographic column body, the both sides of chromatographic column body are equipped with the mount pad, the lateral wall fixedly connected with cylinder of mount pad, sliding connection has four slide bars in the cylinder, four the slide bar is the circumference and distributes, the top fixedly connected with fixture block of slide bar, the fixture block is the arc form, be the circumference in the cylinder and seted up four spouts, sliding connection has the slide in the spout, slide bar fixed connection is at the top of slide, the one end fixedly connected with spring of slide bar is kept away from to the slide, the one end fixed connection of slide is kept away from to the spring in the bottom of spout.
Preferably, four lateral walls of the mounting seat are in threaded connection with short rods, the top of each short rod is fixedly connected with a handle, and the mounting seat is fixedly connected with a rubber plate.
Preferably, four communicated sliding grooves are formed in the mounting seat and the rubber plate, the clamping block is fixedly connected with a connecting rod, the connecting rod is connected in the sliding grooves in a sliding mode, and the bottoms of the short rods are abutted to the connecting rod.
Preferably, one end of the mounting seat, which is far away from the cylinder, is fixedly connected with a hand screwing part, and extension plates are fixedly connected to two sides of the hand screwing part.
Preferably, the mounting seat, the hand screwing part and the cylinder are internally provided with communicated liquid inlets.
Preferably, a connecting pipe is fixedly mounted on the side wall of the cylinder, a splitter plate is fixedly connected to one end, far away from the cylinder, of the connecting pipe, a liquid inlet cavity is formed in the splitter plate, and a liquid outlet hole communicated with the liquid inlet cavity is formed in the splitter plate.
Compared with the prior art, the utility model provides a liquid chromatography column connects possesses following beneficial effect:
1. this liquid chromatography column connects, when installing the mount pad on the chromatographic column body, the extrusion fixture block, the fixture block drives the slide bar and moves down, the slide bar drives the slide and removes in the spout, the extrusion spring when slide moves down, exert pressure to the spring, then remove the mount pad to this internal shift of chromatographic column, loosen the fixture block, the fixture block moves up under the elastic deformation of spring, support the inner wall of chromatographic column body, fix on the chromatographic column body, accomplish the installation, can be according to the distance of the size of a dimension regulation fixture block joint of different chromatographic column bodies, be fit for installing on the liquid chromatography column of different sizes.
2. This liquid chromatography column connects, on the liquid that needs the test enters into the flow distribution plate through the inlet, then spray on the inner wall of chromatographic column body from the play liquid hole of flow distribution plate, can be so that the comparatively even that the liquid that awaits measuring sprays, the expansion of the test work of being convenient for improves work efficiency.
The part that does not relate to among the device all is the same with prior art or can adopt prior art to realize, the utility model discloses can be fit for installing on the liquid chromatography column of not unidimensional size according to the distance of the size of a dimension regulation fixture block joint of different chromatographic column bodies.
Drawings
Fig. 1 is a schematic structural diagram of a liquid chromatography column joint according to the present invention;
fig. 2 is a schematic structural diagram of a liquid chromatography column joint mounting seat according to the present invention;
fig. 3 is a schematic cross-sectional structural view of a liquid chromatography column joint mounting seat according to the present invention;
fig. 4 is a schematic cross-sectional structural view of a liquid chromatography column joint splitter plate according to the present invention;
fig. 5 is a schematic side view of a liquid chromatography column joint mounting seat according to the present invention;
fig. 6 is a schematic cross-sectional structural view of a liquid chromatography column joint cylinder according to the present invention;
fig. 7 is a schematic side sectional view of a liquid chromatography column joint mounting base according to the present invention;
fig. 8 is a schematic structural diagram of a portion a in fig. 6 of a liquid chromatography column joint according to the present invention.
In the figure: 1. a chromatography column body; 2. a mounting seat; 3. a rubber plate; 4. a hand-wringing portion; 5. an extension plate; 6. a short bar; 7. a handle; 8. a cylinder; 9. a clamping block; 10. a connecting rod; 11. a sliding groove; 12. a slide bar; 13. a chute; 14. a slide plate; 15. a spring; 16. a liquid inlet; 17. a connecting pipe; 18. a flow distribution plate; 19. a liquid inlet cavity; 20. and a liquid outlet hole.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Example (b):
referring to fig. 1-8, a liquid chromatography column connects, including chromatographic column body 1, chromatographic column body 1's both sides are equipped with mount pad 2, the lateral wall fixedly connected with cylinder 8 of mount pad 2, sliding connection has four slide bars 12 in the cylinder 8, four slide bars 12 are the circumference and distribute, the top fixedly connected with fixture block 9 of slide bar 12, fixture block 9 is the arc form, be the circumference in the cylinder 8 and seted up four spout 13, sliding connection has slide 14 in the spout 13, slide bar 12 fixed connection is at the top of slide 14, slide 14 keeps away from the one end fixedly connected with spring 15 of slide bar 12, the one end fixed connection at the bottom of spout 13 that slide 14 was kept away from to spring 15.
The equal threaded connection of four lateral walls of mount pad 2 has quarter butt 6, and the top fixedly connected with handle 7 of quarter butt 6, fixedly connected with rubber slab 3 on the mount pad 2, and the lateral wall of chromatographic column body 1 is hugged closely to rubber slab 3, forms airtight space, prevents that gas leakage from leading to the liquid outflow of test.
Four communicating sliding tray 11 have been seted up on mount pad 2 and the rubber slab 3, fixedly connected with connecting rod 10 on the fixture block 9, connecting rod 10 sliding connection is in sliding tray 11, the bottom of quarter butt 6 offsets in connecting rod 10, when needs dismantle mount pad 2, rotate quarter butt 6, quarter butt 6 removes in to sliding tray 11, connecting rod 10 on the drive fixture block 9 moves down, connecting rod 10 drives fixture block 9 and moves down, fixture block 9 has just broken away from chromatographic column body 1's inner wall like this, then take out it, accomplish the dismantlement.
A liquid inlet 16 communicated with each other is arranged in the mounting seat 2, the hand-screwing part 4 and the column 8.
The lateral wall fixed mounting of cylinder 8 has connecting pipe 17, the one end fixedly connected with flow distribution plate 18 of cylinder 8 is kept away from to connecting pipe 17, feed liquor chamber 19 has been seted up in flow distribution plate 18, set up in flow distribution plate 18 with the communicating play liquid hole 20 in feed liquor chamber 19, the liquid that needs the test passes through inlet 16 and gets into in connecting pipe 17, then on entering flow distribution plate 18, then go out liquid hole 20 from flow distribution plate 18 and spray on the inner wall of chromatographic column body 1, can make the comparatively even that the liquid that awaits measuring sprays, the expansion of the test work of being convenient for, and the work efficiency is improved.
The utility model discloses in, when installing mount pad 2 on chromatographic column body 1, extrude fixture block 9, fixture block 9 drives slide bar 12 downstream, slide bar 12 drives slide 14 and removes in 13 spouts in the cylinder 8, slide 14 extrudees spring 15 when removing downwards, exert pressure to spring 15, spring 15 applys thrust to slide 14, then remove mount pad 2 and remove in the pipe wall of chromatographic column body 1, it hugs closely to remove rubber slab 3 on the mount pad 2, loosen fixture block 9, slide 14 drives slide bar 12 rebound under spring 15's elastic deformation, drive the inner wall that fixture block 9 supported chromatographic column body 1, fix on chromatographic column body 1, accomplish the installation, can adjust the distance of fixture block 9 joint according to the size of a dimension of different chromatographic columns, be fit for installing on the liquid chromatographic column of different sizes.
The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.
Claims (6)
1. The utility model provides a liquid chromatography column connects, a serial communication port, including chromatographic column body (1), the both sides of chromatographic column body (1) are equipped with mount pad (2), lateral wall fixedly connected with cylinder (8) of mount pad (2), sliding connection has four slide bars (12), four in cylinder (8) sliding connection has slide bar (12) to be the circumference to distribute, the top fixedly connected with fixture block (9) of slide bar (12), fixture block (9) are the arc form, be the circumference in cylinder (8) and seted up four spout (13), sliding connection has slide (14) in spout (13), slide bar (12) fixed connection is at the top of slide (14), slide (14) keep away from the one end fixedly connected with spring (15) of slide bar (12), the one end fixed connection of slide (14) is kept away from in spring (15) is in the bottom of spout (13).
2. The liquid chromatography column joint as claimed in claim 1, wherein the four side walls of the mounting seat (2) are respectively connected with a short rod (6) in a threaded manner, the top of the short rod (6) is fixedly connected with a handle (7), and the mounting seat (2) is fixedly connected with a rubber plate (3).
3. The liquid chromatography column joint of claim 2, wherein the mounting seat (2) and the rubber plate (3) are provided with four communicated sliding grooves (11), the fixture block (9) is fixedly connected with a connecting rod (10), the connecting rod (10) is slidably connected in the sliding grooves (11), and the bottom of the short rod (6) is abutted against the connecting rod (10).
4. The liquid chromatography column joint of claim 1, wherein one end of the mounting seat (2) far away from the column (8) is fixedly connected with a hand-screwed part (4), and extension plates (5) are fixedly connected with two sides of the hand-screwed part (4).
5. A liquid chromatography column adapter according to claim 4, wherein the mounting base (2), the hand screw (4) and the cylinder (8) are provided with liquid inlets (16) communicating with each other.
6. The liquid chromatography column joint according to claim 5, wherein a connecting pipe (17) is fixedly installed on the side wall of the cylinder (8), a splitter plate (18) is fixedly connected to one end of the connecting pipe (17) far away from the cylinder (8), a liquid inlet cavity (19) is formed in the splitter plate (18), and a liquid outlet hole (20) communicated with the liquid inlet cavity (19) is formed in the splitter plate (18).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221990806.6U CN218036624U (en) | 2022-07-30 | 2022-07-30 | Liquid chromatography column joint |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221990806.6U CN218036624U (en) | 2022-07-30 | 2022-07-30 | Liquid chromatography column joint |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218036624U true CN218036624U (en) | 2022-12-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221990806.6U Active CN218036624U (en) | 2022-07-30 | 2022-07-30 | Liquid chromatography column joint |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218036624U (en) |
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2022
- 2022-07-30 CN CN202221990806.6U patent/CN218036624U/en active Active
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