CN213769564U - Kit for sorting CD3+ T cells - Google Patents
Kit for sorting CD3+ T cells Download PDFInfo
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- CN213769564U CN213769564U CN202022463344.XU CN202022463344U CN213769564U CN 213769564 U CN213769564 U CN 213769564U CN 202022463344 U CN202022463344 U CN 202022463344U CN 213769564 U CN213769564 U CN 213769564U
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Abstract
The utility model discloses a CD3+Kit that T cell was selected separately, including box body and the lid that can open and shut, the box body includes that the consumptive material places the district, the reagent places the district, the sample places the district and the district that stews of cell separation, it places the hole to be equipped with the reagent container in the reagent places the district, the reagent includes CD3 magnetic bead and sorting buffer solution, cell separation is stood and is distinguished one side and inlayed the magnet piece, the magnet piece is connected with the adsorption column draw-in groove, cell separation is stood and is stoodDistrict right side opening still includes through the opening discrepancy the cell separation place the district inner chamber that stews remove the standing groove and with the push-and-pull plate that the removal standing groove is connected, remove the standing groove with cell separation place district sliding connection that stews, the push-and-pull plate with opening shape fit and with the box body can open and shut the connection, be equipped with the collecting pipe rack in the removal standing groove. The utility model provides a perfect system, complete set, practicality and convenience, easy operation's being used for sorting CD3+T cell kit.
Description
Technical Field
The utility model belongs to the technical field of the kit, concretely relates to use magnetic bead to select separately CD3+T cell kit.
Background
Peripheral Blood Mononuclear Cells (PBMC) are cells with a single nucleus in Peripheral blood, including lymphocytes and monocytes. Lymphocytes are a type of cells with immune recognition function, and can be classified into T lymphocytes (also known as T cells), B lymphocytes (also known as B cells), and natural killer cells (NK cells).
CD3 is expressed on all T cells. 70-80% of human peripheral blood lymphocytes and 65-85% of thymocytes are CD3+In (1).
A chimeric antigen receptor T cell (CAR-T cell) is an immunotherapy for effectively treating malignant tumor of a blood system, a specific chimeric antigen receptor is constructed, a gene for coding the chimeric antigen receptor is inserted into the T cell to be expressed through a certain way, then the CAR-T cell is amplified in vitro and finally returned to a patient, and the CAR-T cell activates cellular immunity through specific recognition of a target antigen, kills tumor cells and achieves the aim of resisting tumor. PBMC isolation from peripheral blood and sorting CD3+T cells are key to the production of CAR-T cells.
Sorting CD3+T cells are mainly separated by a magnetic bead sorting method, namely, specific monoclonal antibodies connected with magnetic beads are combined with cell surface antigens, and cells combined with the magnetic beads and cells not combined with the magnetic beads are separated in an external magnetic field. When the existing magnetic bead sorting technology is used for sorting cells, reagents and consumables from various sources need to be used under specific conditions, the operation is inconvenient, the speed is low, and the efficiency is low.
Disclosure of Invention
The utility model aims to solve the technical problem that a kit for CD3+ T cell sorting is provided, which has the advantages of complete system, complete set, convenient use and simple operation.
In order to solve the above problem, the utility model discloses the technical scheme who adopts does:
the utility model provides a CD3+ T cell sorting kit includes the box body and rather than the lid that opens and shuts and be connected, it places the district to be provided with the consumptive material in the box body, the reagent places the district, the sample is placed the district and the district is stood in cell sorting, the reagent is placed and is equipped with the reagent container in the district and place the hole, the reagent includes CD3 magnetic bead and sorting buffer solution, cell sorting is stood and is distinguished one side and has inlayed the magnet piece, the magnet piece is connected with the adsorption column draw-in groove, cell sorting is stood and is distinguished right side opening, still includes through the opening discrepancy the cell sorting is stood and is distinguished the inner chamber remove the standing groove and with remove the push-and-pull plate that the standing groove is connected, remove the standing groove with cell sorting is stood and is distinguished sliding connection, the push-pull plate with opening shape cooperation and with the box body connection that can open and shut, be equipped with the collecting pipe in the removal standing groove.
Preferably, a plurality of sample container placing holes are formed in the sample placing area, an ice bag placing groove is formed below the sample placing area, the outer side of the ice bag placing groove is open, and ice bags can be taken and placed through the opening.
Preferably, the ice bag standing groove inner wall is equipped with the sponge (melamine sponge) of certain thickness, has certain heat-proof quality, still has better hygroscopicity, and the setting of sponge does not influence the placing of ice bag.
Preferably, the magnet piece with the adsorption column draw-in groove can be dismantled and be connected, magnet piece inside wall is equipped with the spout, and adsorption column draw-in groove back is equipped with and follows the gliding slider of spout.
Preferably, the number of the magnet blocks is 2, and the number of the adsorption column clamping grooves is 2.
Preferably, a pull ring is arranged on a push-pull plate connected with the movable placing groove, and a slide rail capable of being clamped with the bottom plate of the movable placing groove is arranged on the side wall of the cell sorting and standing area.
Preferably, the consumable comprises an adsorption column and a collection tube.
Preferably, the adsorption column comprises an adsorption column body and an adsorption column pusher matched with the adsorption column body, and the adsorption column body has magnetism.
Preferably, the upper surface of the bottom plate of the movable placing groove is provided with a limiting block which can fix the collecting pipe placing rack.
Preferably, the size of the reagent bottle containing the sorting buffer solution is 50mL, and the number of the reagent bottles is 2; the specification of the reagent bottle containing the CD3 magnetic beads is 1mL, and the number of the reagent bottles is 1 bottle.
Preferably, the specification of the sample tube is 50ml, and the specification of the collecting tube is 15 ml.
Compared with the prior art, the utility model discloses the beneficial effect who reaches:
(1) the kit provides a plurality of adsorption column clamping grooves, can be used for simultaneously carrying out a plurality of cell sorting experiments, greatly improves the experiment efficiency and saves the time required by the experiments.
(2) This kit provides the adsorption column draw-in groove that can freely dismantle and install, and it is convenient to use during the experiment, and detachable design can prevent the difficult condition of taking of adsorption column because of magnet suction too big leads to, convenient and practical.
(3) The kit is provided with an ice groove, and the inner wall of the ice groove is provided with a foam material with a certain thickness, so that the reagent and the cell sample are placed in the environment and kept at low temperature for a certain time.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
FIG. 2 is a schematic view of the internal structure of the sample placement section and the ice bag placement groove in front view;
FIG. 3 is a schematic top view of the magnet block and the clamping groove of the adsorption column;
description of reference numerals: 1. the lid, 2, the box body, 3, the ice bag standing groove, 4, the magnet piece, 5, the district is placed to reagent, 6, the district is placed to the sample, 7, cell separation the district that stews, 8, the district is placed to the consumptive material, 9, the collecting pipe rack, 10, the pull ring, 11, the push-and-pull board, 12, the opening, 13, the hole is placed to the sample container, 14, the sponge, 15, the adsorption column draw-in groove, 16, the stopper, 17, the baffle, 18, adsorption column and collecting pipe, 19, the hole is placed to the reagent container, 20, the spout, 21, the slider.
Detailed Description
The present invention will be further described with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.
It should be noted that, in the description of the present invention, the terms "front", "rear", "left", "right", "upper", "lower", "inner", "outer", etc. indicate the directions or positional relationships based on the directions or positional relationships shown in the drawings, and are only for convenience of description of the present invention but do not require the present invention to be constructed and operated in a specific direction, and thus, should not be construed as limiting the present invention. As used in the description of the present invention, the terms "front," "back," "left," "right," "up," "down" and "in" refer to directions in the drawings, and the terms "inner" and "outer" refer to directions toward and away from, respectively, the geometric center of a particular component.
As shown in fig. 1, a CD3+T cell sorting kit, including box body 2 and one side with box body 2 articulated, lid 1 that another side and box body buckle are connected. Box body 2 sets gradually the consumptive material from a left side to the right side and places district 8, reagent and place district 5, sample and place district 6 and cell separation district 7 that stews, the consumptive material is placed district 8 and is set up baffle 17, and consumptive material adsorption column and collecting pipe 18 are placed in order, the equal single aseptic wrapping bag that has all been wrapped of adsorption column and collecting pipe 18 reduces pollution. The reagent placing area is provided with a reagent container placing hole 19 for respectively accommodating the magnetic beads of the reagent CD3 and the sorting buffer.
As shown in fig. 1 and 2, a plurality of sample container placing holes 13 are arranged in the sample placing area, an ice bag placing groove 3 is arranged below the sample placing area 6, an opening 12 is arranged on one side of the ice bag placing groove 3, an ice bag can be placed and taken through the opening 12, melamine sponge 14 with a certain thickness is arranged on the inner wall of the ice bag placing groove 3, the material has certain heat insulation property and better hygroscopicity, and the low-temperature environment in the ice groove can be maintained; this ice bag standing groove 3 locates reagent and places 5 right sides in district and sample and place the district below, can provide certain low temperature environment for reagent and sample simultaneously.
As shown in fig. 1 and 3, the cell sorting and standing area 7 is embedded with a magnet block 4, the magnet block 4 is detachably connected with the adsorption column clamping groove 15, the inner side wall of the magnet block 4 is provided with a sliding groove 20, and the back of the adsorption column clamping groove 15 is provided with a sliding block 21 which can slide along the sliding groove. 7 right side openings in district that stews are selected separately to cell still include through the opening discrepancy the cell is selected separately and is stetted the removal standing groove of 7 inner chambers in district and with the push-and-pull plate 11 that the removal standing groove is connected, remove the standing groove with 7 sliding connection in district that stews is selected separately to cell, push-and-pull plate 11 with the opening shape cooperation and with box body 2 can open and shut and connect, be equipped with collecting pipe rack 9 in the removal standing groove.
As shown in fig. 1, a pull ring 10 is arranged on a push-pull plate connected with the mobile placement groove, a slide rail capable of being clamped with a bottom plate of the mobile placement groove is arranged on the side wall of the cell sorting standing region 7, the mobile placement groove can be pulled out through the pull ring 10 on the push-pull plate to observe the cell separation condition, and the experimental operation space is increased; after sorting is finished, the collecting pipe placing frame 9 can be directly taken out for subsequent experiments. Is beneficial to the subsequent experimental operation.
The consumable comprises an adsorption column and a collecting pipe 18, wherein the adsorption column comprises an adsorption column body and an adsorption column pusher matched with the adsorption column body, and the adsorption column body has magnetism.
As shown in fig. 1, the upper surface of the bottom plate of the movable placing groove is provided with a limiting block 16 which can fix the collecting pipe placing rack.
The use mode of the kit provided by the embodiment is as follows: in the experiment of separating CD3+ T cells by magnetic beads, PBMC cells are taken out from liquid nitrogen before the experiment is started, are quickly placed in a water bath kettle at 37 ℃ for melting, are immediately added into a culture medium prepared in advance, and are used for standby after centrifugal counting; centrifuging, removing supernatant, adding sorting buffer solution and CD3 magnetic beads prepared in the kit, fully mixing uniformly, placing in a sample placing area 6, standing for 15min, and centrifuging and resuspending for later use; the adsorption column clamping groove 15 is connected with the magnet block 4, and then the adsorption column is placed on the adsorption column clamping groove 15. Slightly pulling out the movable placing groove, placing the collecting pipe in the collecting pipe frame 9, clamping in the movable placing groove, pushing the movable placing groove into the cell sorting and standing area 7, and wetting and washing the adsorption column by using a sorting buffer solution, wherein the collecting pipe is just positioned under the adsorption column; the cells to be used were then mixed well and added to the adsorption column, taking care not to generate air bubbles. And then cleaning the adsorption column, after cleaning, detaching the adsorption column clamping groove 15 from the magnet block 4, taking a clean cell collecting tube, placing the cell collecting tube in the sample placing area 6, taking down the adsorption column, placing the adsorption column on the cell collecting tube, adding 5mL of separation buffer solution into the adsorption column, and carefully pushing out the liquid by pushing the adsorption column.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be considered as the protection scope of the present invention.
Claims (9)
1. A kit for sorting CD3+ T cells comprises a box body and a box cover which can be opened and closed, it is characterized in that a consumable material placing area, a reagent placing area, a sample placing area and a cell sorting and standing area are arranged in the box body, a reagent container placing hole is arranged in the reagent placing area, the reagent comprises CD3 magnetic beads and sorting buffer solution, a magnet block is embedded at one side of the cell sorting and standing area, the magnet block is connected with an adsorption column clamping groove, the right side of the cell sorting and standing area is provided with an opening, the cell sorting and standing area also comprises a moving placing groove which is in and out of the inner cavity of the cell sorting and standing area through the opening and a push-pull plate which is connected with the moving placing groove, the movable placing groove is in sliding connection with the cell sorting and standing area, the push-pull plate is matched with the opening in shape and can be connected with the box body in an opening and closing mode, and a collecting pipe placing frame is arranged in the movable placing groove.
2. The kit for sorting the CD3+ T cells according to claim 1, wherein a plurality of sample container placing holes are arranged in the sample placing area, an ice bag placing groove is arranged below the sample placing area, one side of the ice bag placing groove is open, and the ice bag can be taken and placed through the opening.
3. The kit for sorting the CD3+ T cells according to claim 2, wherein a layer of sponge is arranged on the inner wall of the ice bag placing groove.
4. The kit for sorting CD3+ T cells according to claim 1, wherein the magnet is detachably connected to the adsorption column slot.
5. The kit for sorting the CD3+ T cells, according to claim 4, wherein the magnet block has a slide slot on its inner side wall, and the adsorption column has a slide block on its back capable of sliding along the slide slot.
6. The kit for sorting the CD3+ T cells according to claim 4, wherein the number of the magnet blocks is 2, and the number of the adsorption column slots is 2.
7. The kit for sorting CD3+ T cells according to claim 1, wherein the push-pull plate connected to the movable placement groove is provided with a pull ring, and the sidewall of the cell sorting resting area is provided with a slide rail capable of engaging with the bottom plate of the movable placement groove.
8. The kit for sorting CD3+ T cells according to claim 1, wherein the consumables comprise adsorption columns and collection tubes.
9. The kit of claim 8, wherein the adsorption column comprises an adsorption column body and an adsorption column pusher matched with the adsorption column body, and the adsorption column body has magnetism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022463344.XU CN213769564U (en) | 2020-10-30 | 2020-10-30 | Kit for sorting CD3+ T cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022463344.XU CN213769564U (en) | 2020-10-30 | 2020-10-30 | Kit for sorting CD3+ T cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213769564U true CN213769564U (en) | 2021-07-23 |
Family
ID=76912657
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022463344.XU Active CN213769564U (en) | 2020-10-30 | 2020-10-30 | Kit for sorting CD3+ T cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213769564U (en) |
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2020
- 2020-10-30 CN CN202022463344.XU patent/CN213769564U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Suzhou Heyu Finance Leasing Co.,Ltd. Assignor: SUZHOU MAXIMUM BIO-TECH Co.,Ltd. Contract record no.: X2023980036580 Denomination of utility model: A kit for CD3+T cell sorting Granted publication date: 20210723 License type: Exclusive License Record date: 20230614 |
|
| EE01 | Entry into force of recordation of patent licensing contract | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A kit for CD3+T cell sorting Effective date of registration: 20230628 Granted publication date: 20210723 Pledgee: Suzhou Heyu Finance Leasing Co.,Ltd. Pledgor: SUZHOU MAXIMUM BIO-TECH Co.,Ltd. Registration number: Y2023980046452 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |