CN213456350U - Push-down vibrates type purifier and purifies post - Google Patents
Push-down vibrates type purifier and purifies post Download PDFInfo
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- CN213456350U CN213456350U CN202020843439.1U CN202020843439U CN213456350U CN 213456350 U CN213456350 U CN 213456350U CN 202020843439 U CN202020843439 U CN 202020843439U CN 213456350 U CN213456350 U CN 213456350U
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Abstract
The utility model relates to a push-down vibrates type purifier and purifies post. The device comprises a hollow column tube, a core rod, a piston, a sieve plate, a purified liquid outflow channel and a plugging piece, wherein the piston is positioned in a cavity of the hollow column tube and is propped against the inner side wall of the hollow column tube along the circumferential direction; the core rod is connected with the upper end surface of the piston so as to drive the piston to move along the axial direction of the hollow column tube; the purified liquid outflow channel is arranged at the bottom of the hollow column tube and is communicated with the cavity of the hollow column tube, and the inner diameter of the purified liquid outflow channel is smaller than that of the hollow column tube; the sieve plate is positioned in the cavity of the hollow column tube so as to prevent the purification filler in the hollow column tube from leaking out; the plugging piece is detachably connected to the lower end of the purified liquid outflow channel. The device of the utility model has the advantages of simple and flexible operation, simple structure, reasonable arrangement and low manufacturing cost.
Description
Technical Field
The utility model relates to a QuECHERS (Quick, Easy, Cheap, effect, Rugged, Safe, Quick, simple, economy, high efficiency, reliable) technical field, concretely relates to push-down vibrates type purifier and purifying column.
Background
The QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) technology is a rapid sample pretreatment technology for food detection of grains, vegetables and the like which is developed internationally in recent years, is developed by American Ministry of agriculture, professor Anastasiades equal to 2003, is already widely used in the field of food detection at present, and has relevant detection standards in the United states and European Union.
The technology mainly comprises three steps of shaking acetonitrile under the action of QuEChERS extraction salting-out package to be extracted, centrifuging, purifying supernatant by adopting a purifying filler and concentrating and redissolving. In 2018, 21.06 and 21, the state also issues a national detection standard GB 23200.1132018 national food safety standard for determination of residual amounts of 208 pesticides and metabolites thereof in plant-derived food based on the QuEChERS technology. The specific pretreatment steps are as follows:
the extraction method comprises the following steps: weighing a sample to be detected in a plastic centrifuge tube, adding acetonitrile, a QuEChERS extraction salting-out bag (magnesium sulfate, sodium chloride, sodium citrate and disodium hydrogen citrate) and 1 ceramic homogeneous proton, covering a centrifuge tube cover, violently shaking and centrifuging;
purification step: sucking a certain amount of supernatant fluid, adding the supernatant fluid into a plastic centrifuge tube containing magnesium sulfate and ethylenediamine-N-propyl silanized silica gel, adding the magnesium sulfate, the ethylenediamine-N-propyl silanized silica gel and graphitized carbon black into the plastic centrifuge tube for a sample with a darker color, and centrifuging after uniformly mixing in a vortex manner;
③ concentration and redissolution step: accurately sucking a certain amount of supernatant into a test tube, blowing nitrogen gas in a water bath at 40 ℃ until the supernatant is nearly dry, redissolving the supernatant, filtering the redissolved supernatant through a microporous filter membrane, and measuring the supernatant by using a machine.
However, the purification steps based on the QuEChERS sample pretreatment technology at present are generally that acetonitrile, a matrix to be detected and a purification filler are mixed and violently vibrated, and finally the mixture is placed in a centrifuge and then centrifuged to obtain an upper layer extracting solution, so that the operation is complex.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model aims at providing a push-down vibrates type purifier and purification post to make the device easy and simple to handle nimble, simple structure, set up reasonable, cost of manufacture low grade.
The utility model provides a push rod type oscillation purification device, which comprises a hollow column tube, a core rod, a piston, a sieve plate, a purified liquid outflow channel and a plugging piece, wherein,
the piston is positioned in the cavity of the hollow cylindrical pipe and is propped against the inner side wall of the hollow cylindrical pipe along the circumferential direction;
the core rod is connected with the upper end surface of the piston so as to drive the piston to move along the axial direction of the hollow column tube;
the purified liquid outflow channel is arranged at the bottom of the hollow column tube and is communicated with the cavity of the hollow column tube, and the inner diameter of the purified liquid outflow channel is smaller than that of the hollow column tube;
the sieve plate is positioned in the cavity of the hollow column tube so as to prevent particles in the hollow column tube from leaking out;
the plugging piece is detachably connected to the lower end of the purified liquid outflow channel.
The device enables the purification filler to be placed in the hollow column tube, the sample extracting solution to be purified is injected through the sample adding hole, vortex oscillation is carried out, and the processed sample can be pushed out from the purification liquid outflow channel through the piston after the processing is finished, so that the further operation is facilitated.
According to the utility model discloses an embodiment, the device still includes the gland, the gland with the upper end fixed connection of core bar.
According to the utility model discloses an embodiment, at least one application of sample hole has been seted up on the lateral wall of hollow pillar pipe, application of sample hole is located the first half of hollow pillar pipe lateral wall.
According to the utility model discloses an embodiment, the shutoff piece includes the indent closing cap, the lower extreme detachably of purifying liquid outflow passageway is inserted and is established in the indent closing cap.
According to the utility model discloses an embodiment, the lower extreme of purifying liquid outflow passageway with the indent closing cap passes through threaded connection, socket joint connection or connects through the joint.
According to the utility model discloses an embodiment, the material of piston is rubber.
According to the utility model discloses an embodiment, the sieve is the porous sieve of hydrophobicity, preferred high molecular weight polytetrafluoroethylene material.
According to the utility model discloses an embodiment, the upper end of hollow column pipe is open structure.
According to the utility model discloses an embodiment, hollow column pipe is the polyethylene PE material.
The utility model also provides a push-down vibrates type and purifies post, including purifying pack with push-down vibrate type purifier, purify the filler and be located the higher authority of sieve.
According to an embodiment of the present invention, the purification filler is any combination of one or more of octadecyl bonded silica gel, graphitized carbon black, anhydrous magnesium sulfate, amino-modified silica gel, ethylenediamine-N-propylsilanized silica gel, polyamide powder, anionic resin particles, cationic resin particles, neutral alumina, graphene, carbon nanotubes, and zirconia particles.
The utility model provides a simple structure, reasonable in design, convenient to use's push-down vibrates type and purifies the post, treats to purify the manual vibration of sample extract accessible or mechanical vortex vibrate and purify the filler misce bene edulcoration.
The clear liquid outflow passageway is located the bottom of hollow pillar pipe, and with the cavity intercommunication of hollow pillar pipe, the internal diameter of clear liquid outflow passageway is less than the internal diameter of hollow pillar pipe is for more easily when releasing the sample extract after purifying, be difficult to with the intraductal purification filler discharge of hollow pillar.
The utility model provides a push-down vibrates type and purifies post, the accessible sets up the indent closing cap, realizes treating the shutoff of purifying sample extract, can select to seal purifying liquid outflow channel with the indent closing cap and then vibrate mixing or manual the vibration at the vortex appearance and shake evenly, and manual promotion piston is one step filtered realization solid-liquid separation, has improved work efficiency.
In addition, the push-rod type oscillation purification column provided by the utility model can inject the sample extract to be purified into the column tube through the sampling hole by arranging the sampling hole on the side wall of the hollow column tube, so that the whole piston does not need to be taken out of the hollow column tube; the sample extracting solution to be purified can be sucked into the column tube through the purified liquid outflow channel by pulling the piston, so that the operation process is facilitated, and the method has strong practicability.
Drawings
Fig. 1 is a schematic structural view of a first push-rod type oscillating purification column according to an embodiment of the present invention;
fig. 2 is a schematic structural view of a second push-rod type oscillating purification column according to an embodiment of the present invention;
reference numerals:
100 hollow column tubes; 101 a sample adding hole;
200 core rods; 300 a piston;
400 purifying filler; 500 purge flow out of the channel;
501, screening a plate; 601, a concave sealing cover;
700, pressing the cover.
Detailed Description
The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings so that the objects, features and advantages of the invention can be more clearly understood. It should be understood that the embodiments shown in the drawings are not intended to limit the scope of the present invention, but are merely intended to illustrate the spirit of the technical solution of the present invention.
The utility model provides a push-down vibrates type and purifies post, the device mainly in order to carry out sample pretreatment operation portably, for example purify the operation such as to the sample extract.
As shown in fig. 1, the push rod type oscillation purification column of the present invention mainly comprises a hollow column tube 100, a core rod 200, a piston 300, a sample adding hole 101, a sieve plate 501, a purified liquid outflow channel 500 and a plugging member, wherein,
the piston 300 is located in the cavity of the hollow cylindrical tube 100 and abuts against the inner side wall of the hollow cylindrical tube 100 along the circumferential direction;
the core bar 200 is connected to an upper end surface of the piston 300 to drive the piston 300 to move in an axial direction of the hollow cylinder 100;
the sampling hole is positioned on the upper half part of the side wall of the hollow column tube;
the purified liquid outflow channel 500 is arranged at the bottom of the hollow column tube 100 and is communicated with the cavity of the hollow column tube 100, and the inner diameter of the purified liquid outflow channel 500 is smaller than that of the hollow column tube 100;
the sieve plate 501 is positioned in the cavity of the hollow column tube 100 (the porous sieve plate is positioned at the bottom of the hollow column tube as shown in fig. 1 and 2);
the plugging piece is detachably connected to the lower end of the purified liquid outflow channel.
The above-described device makes it possible to insert the purification packing 400 into the hollow column 100 and to inject the extraction liquid of the sample to be treated, and after the treatment is completed, the treated sample to be measured can be pushed out from the purification liquid outflow passage by the piston for further operation.
Specifically, the hollow pillar 100 may be a circular pillar or a square pillar. A piston 300 is movably installed in the hollow cylindrical tube 100, and the piston 300 moves in an axial direction (in fig. 1 and 2, in an up-and-down direction) along an inner sidewall of the hollow cylindrical tube 100 by pushing and pulling the core rod 200. The bottom of the hollow column tube is provided with a purification liquid outflow channel 500 which is communicated with the cavity of the hollow column tube 100 and protrudes downwards, the bottom of the cavity can contain the purification filler 400, and the bottom of the hollow column tube 100 is provided with a porous sieve plate 501 for plugging the purification filler 400.
Alternatively, the top of the hollow cylinder 100 may be an open structure, or a sample hole may be formed at the top, so that the core rod 200 may pass through the sample hole to drive the piston 300 to move.
Alternatively, the structure of the piston 300, the hollow column tube 100 and the purified liquid outflow passage 500 is a syringe type structure.
According to an embodiment of the present invention, the device further comprises a gland 700, the gland 700 is fixedly connected to the upper end of the core rod 200. As shown in fig. 1, the lower surface of the gland 700 is fixedly coupled to the upper end of the stem 200. The provision of the gland 700 may allow the operator to more easily move the piston.
As shown in fig. 1, according to an embodiment of the present invention, at least one sample hole 101 is opened on the sidewall of the hollow column 100, and the sample hole is located on the upper half of the sidewall of the hollow column, that is, the sample hole is located above the purification packing, so that the sample extract to be processed can be injected from the hole. The side wall of the hollow column tube 100 is provided with the sample adding hole, so that the sample extracting solution to be detected can be injected from the sample adding hole, the dosage requirement of the sample extracting solution to be detected is reduced, and the operation is more convenient.
The utility model provides a push-down vibrates type and purifies post, the accessible has seted up the application of sample hole on the lateral wall of hollow pillar pipe to can pour into from the application of sample hole and wait to purify the sample extract, thereby need not take out the piston is whole from hollow pillar pipe, made things convenient for operation process, have very strong practicality.
The utility model discloses well shutoff piece detachably connects keeping away from of purifying liquid outflow passageway the one end of hollow column pipe. As shown in fig. 1, according to an embodiment of the present invention, the blocking member includes a concave cover 601, and the lower end of the purified liquid outflow channel is detachably inserted into the concave cover 601.
According to the utility model discloses an embodiment, the lower extreme of purifying liquid outflow passageway with the indent closing cap passes through threaded connection, socket joint connection or connects through the joint.
The utility model provides a push-down vibrates type QuECHERS purifies post, the accessible sets up the indent closing cap, realizes treating the extract shutoff of purifying the sample, can select to seal purifying liquid outflow channel with the indent closing cap and then manual vortex vibrates or at the vortex appearance mixing, and manual promotion piston is one step filtered realization solid-liquid separation, has improved work efficiency.
The utility model discloses in through setting up the indent closing cap, can realize the hybrid process of two kinds of different modes, one kind will be waited to purify the sample extract and utilize the piston to inhale hollow column pipe from purification liquid outflow passageway earlier, then utilize the indent closing cap shutoff, another kind uses indent closing cap shutoff earlier, then will wait to purify the sample extract and pour into from the upper end application of sample hole of hollow column pipe, this structure has greatly enriched the operating possibility, has very strong practicality.
According to an embodiment of the present invention, the piston 300 is preferably made of rubber, and the sieve plate is preferably made of high molecular weight polytetrafluoroethylene with hydrophobic porous sieve plate.
Optionally, the purification filler 400 includes any one or any combination of octadecyl bonded silica gel, graphitized carbon black, anhydrous magnesium sulfate, amino-modified silica gel, ethylenediamine-N-propylsilanized silica gel, polyamide powder, anionic resin particles, cationic resin particles, neutral alumina, graphene, carbon nanotubes, and zirconium dioxide particles, and may be other kinds of purification fillers, which are not particularly limited. The purification material may be a mixture of solid particles without fixed packaging.
When in use, the porous sieve plate 501 is placed at the bottom of the hollow column tube 100; adding the purification filler into the hollow column tube 100, and then pressing in the piston 300 connected with the core rod 200, thus assembling the push rod type oscillation purification column; placing the purified liquid outflow channel 500 into a container filled with a sample extracting solution to be detected, pulling the piston 300 outwards, sucking the sample extracting solution to be detected into the hollow column tube 100 under the action of pulling force of the piston 300 pulling outwards to mix with the purifying filler 400, and shaking by hand shaking to promote the QuEChERS purifying material 400 to adsorb impurities so as to purify the sample extracting solution; after purification is finished, the piston 300 is pushed to directly transfer the extracting solution into a sample bottle, and then next pretreatment such as QuEChERS subsequent steps can be carried out.
Further, the purification filler is purification filler with different specifications, such as a mixture of ethylenediamine-N-propyl silanized silica gel, magnesium sulfate and graphitized carbon black particles recommended in a gas chromatography-mass spectrometry combined method for measuring the residual quantity of 208 pesticides and metabolites thereof in plant-derived food of national standard GB23200.113-2018 food safety national standard, octadecyl bonded silica gel particles recommended in a detection standard NY/T1380 secondary 2007 vegetable of agricultural industry, a gas chromatography-mass spectrometry method for measuring multiple residues of 51 pesticides in fruits, a mixture of ethylenediamine-N-propyl silanized silica gel and anhydrous magnesium sulfate, a degreasing dispersion purifying agent recommended in a liquid chromatography-tandem mass spectrometry method for measuring 160 veterinary drugs and other compounds in livestock blood and urine of national detection standard 'Ministry of agriculture' No. 197-10-2019, Neutral alumina and other types of purification fillers. The application is not limited thereto.
Preferably, the purifying filler is one or more of octadecyl bonded silica gel, graphitized carbon black, anhydrous magnesium sulfate, amino modified silica gel, ethylenediamine-N-propyl silanized silica gel, polyamide powder, anionic resin particles, cationic resin particles, neutral alumina, anhydrous magnesium sulfate, graphitized carbon black, neutral alumina particles, graphene, carbon nanotubes, zirconium dioxide particles and the like in any combination.
The utility model provides a simple structure, reasonable in design, convenient to use's push-down vibrates type and purifies the post, treats to purify the manual vibration of sample extract accessible or mechanical vortex vibrate and purify the filler misce bene edulcoration.
The following examples are set forth.
Example 1
Fig. 1 is a schematic structural view of a first push rod type oscillation type purification column, and the following description will specifically describe this embodiment with reference to fig. 1.
As shown in fig. 1, the push rod type oscillation purification column includes a hollow column tube 100, a sample adding hole 101, a core rod 200, a piston 300, a purification packing 400, a purification liquid outflow channel 500, and a gland 700, wherein a porous sieve plate 501 is disposed in the purification liquid outflow channel 500, and the sample adding hole 101 is located above the purification packing 400.
The operation of the push-rod oscillation-type purification column shown in fig. 1 is described as follows:
(1) placing the porous sieve plate 501 at the bottom of the hollow column tube 100;
(2) adding a mixture of ethylenediamine-N-propyl silanized silica gel, magnesium sulfate and graphitized carbon particles or a purification filler 400 consisting of the mixture and the like into the hollow column tube 100, and then pressing in the piston 300 connected with the core rod 200 and the gland 700, so as to assemble the push rod type oscillation purification column;
(3) loading: the extraction solution to be purified is moved into the hollow column tube 100 through the channel 101 (specifically, the piston 300 connected with the core rod 200 and the gland 700 is firstly pulled to the upper part of the sample adding hole 101, then the sample extraction solution to be purified is injected into the sample adding hole 101), then the piston 300 connected with the core rod 200 and the gland 700 is slightly pressed into the lower part of the sample adding hole 101, the push rod type oscillation purification column is manually oscillated or placed on a vortex instrument to be uniformly swirled, and the purification filler 400 is promoted to adsorb impurities so as to purify the sample extraction solution; (4) solid-liquid separation: after uniform mixing, the concave sealing cover 601 of the purified liquid flowing out of the channel 500 is discarded, the piston 300 is pushed to directly transfer the extracting solution into the sample bottle, and the next pretreatment such as the QuEChERS purification step can be carried out.
Example 2
The present embodiment is different from embodiment 1 in that the sample extracting solution to be purified is drawn into the hollow column tube from the purified liquid outflow channel by pulling the piston, and then is blocked by the concave cover. The working process is similar to that in embodiment 1, and is not described in detail here.
Example 3
Fig. 2 is a schematic structural view of a second push rod type oscillation type purification column, and the following description will specifically describe this embodiment with reference to fig. 2.
As shown in fig. 2, the push rod type oscillation purification column includes a hollow column tube 100, a core rod 200, a piston 300, a purification packing 400, a purification liquid outflow channel 500, and a gland 700, wherein a porous sieve plate 501 is disposed in the purification liquid outflow channel 500, and a sample addition hole 101 is located above the purification packing 400.
The present embodiment is different from embodiment 1 in that the side wall of the hollow column tube 100 has no sample addition hole 101, and the sample extraction solution to be purified is drawn into the hollow column tube from the purified liquid outflow channel by the pulling of the piston and then blocked by the concave cover. The working process is similar to that in embodiment 1, and is not described in detail here.
It is noted that, in this document, relational terms such as "first" and "second," and the like, may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The above embodiments are only used for illustrating the present invention, and all the components and devices of the embodiments can be changed, and all equivalent changes and improvements based on the technical solution of the present invention should not be excluded from the scope of the present invention.
Claims (10)
1. A push rod type oscillation type purification device is characterized by comprising a hollow column tube, a core rod, a piston, a sieve plate, a purified liquid outflow channel and a plugging piece, wherein,
the piston is positioned in the cavity of the hollow cylindrical pipe and is propped against the inner side wall of the hollow cylindrical pipe along the circumferential direction;
the core rod is connected with the upper end surface of the piston so as to drive the piston to move along the axial direction of the hollow column tube;
the purified liquid outflow channel is arranged at the bottom of the hollow column tube and is communicated with the cavity of the hollow column tube, and the inner diameter of the purified liquid outflow channel is smaller than that of the hollow column tube;
the sieve plate is positioned in the cavity of the hollow column tube or in the purified liquid outflow channel so as to prevent purified filler particles in the hollow column tube from leaking out;
the plugging piece is detachably connected to the lower end of the purified liquid outflow channel.
2. The rod-type oscillating purification device according to claim 1, further comprising a gland fixedly connected to the upper end of the core rod.
3. The push-rod type oscillating purification device according to claim 1 or 2, wherein the side wall of the hollow column tube is provided with at least one sample adding hole, and the sample adding hole is positioned on the upper half part of the side wall of the hollow column tube.
4. The pusher-type oscillating purification device according to claim 1 or 2, wherein the block piece comprises a concave cover in which the lower end of the purified liquid outflow channel is detachably inserted.
5. The pusher-type oscillating purification device of claim 4, wherein the lower end of the purification liquid outflow channel is connected with the concave cover by a screw, socket or snap connection.
6. The pusher-type oscillating purification device of claim 1, 2 or 5, wherein the piston is made of rubber.
7. The pusher-bar oscillating purification device of claim 1, 2 or 5, wherein the sieve plate is a hydrophobic porous sieve plate.
8. The pusher-type oscillating purification device according to claim 1, 2 or 5, wherein the upper end of the hollow column tube is an open structure or a semi-closed opening with a sample application hole.
9. A pusher-rod type oscillating purification column, comprising a purification packing and a pusher-rod type oscillating purification device according to any one of claims 1 to 8, wherein the purification packing is located above the sieve plate.
10. The push-rod oscillation-type purification column according to claim 9, wherein the purification filler is octadecyl bonded silica gel or graphitized carbon black or anhydrous magnesium sulfate or amino modified silica gel or ethylenediamine-N-propylsilanized silica gel or polyamide powder or anionic resin particles or cationic resin particles or neutral alumina or graphene or carbon nanotubes or zirconium dioxide particles.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020843439.1U CN213456350U (en) | 2020-05-19 | 2020-05-19 | Push-down vibrates type purifier and purifies post |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020843439.1U CN213456350U (en) | 2020-05-19 | 2020-05-19 | Push-down vibrates type purifier and purifies post |
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| Publication Number | Publication Date |
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| CN213456350U true CN213456350U (en) | 2021-06-15 |
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| CN202020843439.1U Active CN213456350U (en) | 2020-05-19 | 2020-05-19 | Push-down vibrates type purifier and purifies post |
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Address after: 100141 No.150, nanqingta village, Yongding Road, Fengtai District, Beijing Patentee after: CHINESE ACADEMY OF FISHERY SCIENCE Address before: 100141 No.150, Qingta village, Changping District, Beijing Patentee before: CHINESE ACADEMY OF FISHERY SCIENCE |