CN213172376U - Digital PCR microdroplet generation, amplification and detection integrated machine - Google Patents
Digital PCR microdroplet generation, amplification and detection integrated machine Download PDFInfo
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- CN213172376U CN213172376U CN202021733568.1U CN202021733568U CN213172376U CN 213172376 U CN213172376 U CN 213172376U CN 202021733568 U CN202021733568 U CN 202021733568U CN 213172376 U CN213172376 U CN 213172376U
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Abstract
The utility model discloses a digit PCR droplet generates and amplifies and detects all-in-one, including PCR data detection appearance, PCR data detection appearance includes that the PCR droplet generates and amplifies regional and PCR data detection area, the PCR droplet generates and amplifies regional inside cavity setting, and the activity is pegged graft and has droplet generation board seat, droplet generation board seat surface activity runs through the grafting and has a plurality of droplet generation pipe, the one end top and the bottom of droplet generation board seat are provided with closing plate and sealed arch respectively, sealed arch sets up with one section phase-match in droplet generation socle bottom, the PCR droplet generates to amplify regional one end top and offers with closing plate assorted seal groove. The utility model discloses an add the function that the droplet generated and amplified to PCR data detection appearance in, make three kinds of function an organic whole combine, the occupation space of how many equipment of effectual reduction to operate on integrative equipment, increase detection efficiency, improve the detection accuracy.
Description
Technical Field
The utility model belongs to the technical field of micro-fluidic liquid drop digital polymerase chain reaction, in particular to digital PCR droplet generates amplification and detects all-in-one.
Background
The digital PCR (polymerase chain reaction) is an absolute quantitative technology of nucleic acid molecules, the digital PCR system is a system adopting the digital PCR technology for detection, and when liquid drop detection is carried out, a sample and diluent are conveyed to a digital PCR detection chip through a pipeline by a power source to be mixed.
At present, the whole process of completing the polymerase chain reaction needs droplet generation, droplet diffusion and droplet detection, a plurality of devices are needed to be used for operation, and when the devices are operated, the devices on the surface of a workbench are more, so that the device not only occupies a test space, but also affects the detection efficiency.
Therefore, it is necessary to invent a digital PCR droplet generation, amplification and detection all-in-one machine to solve the above problems.
SUMMERY OF THE UTILITY MODEL
To the above problem, the utility model provides a digit PCR droplet generates amplification and detects all-in-one to solve the problem that proposes in the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a digital PCR droplet generates and amplifies and detects all-in-one, includes PCR data detection appearance, PCR data detection appearance includes that the PCR droplet generates and amplifies regional and PCR data detection area, the regional inside cavity setting that amplifies is generated to the PCR droplet, and the activity is pegged graft has droplet to generate the board seat, droplet generates board seat surface activity and runs through the grafting and has a plurality of droplet to generate the pipe, the one end top and the bottom of droplet generation board seat are provided with sealing plate and sealed arch respectively, sealed arch and one section phase-match setting in droplet generation pipe bottom, the regional one end top of PCR droplet generation amplification is seted up with the sealing groove of sealing plate phase-match, be provided with the permanent magnet between sealing plate and the sealing groove, the movable grafting of droplet generation board seat surface one side has the clamp plate, all be provided with first slider between the both sides of clamp plate and the both ends of, the two ends of the droplet generation plate seat are both provided with first sliding grooves matched with the first sliding blocks, the bottom in the PCR droplet generation and amplification area is provided with a droplet generation and amplification heating seat, the inside of the droplet generation and amplification heating seat is provided with a heating resistor, one end of the droplet generation and amplification heating seat, which is positioned in the PCR droplet generation and amplification area, is provided with a second sliding block, one end in the PCR droplet generation and amplification area is provided with a second sliding groove matched with the second sliding block, the bottom of the other end of the bottom of the droplet generation and amplification heating seat is fixedly connected with a sealing sliding plate, the surface of the sealing sliding plate is embedded with a sealing nylon cushion, the inside of the PCR droplet generation and amplification area is provided with a slot matched with the sealing sliding plate, a spring is arranged between the bottom of the sealing sliding plate and the slot, and one side of the, the surface of the sealing sliding plate is provided with a push groove matched with the push rod, and one end of the outer part of the PCR microdroplet generation and amplification area is provided with an L-shaped groove matched with the push rod.
Furthermore, one end of the droplet generation plate seat, which is positioned in the PCR droplet generation amplification area, is provided with a lengthening plate, third sliding grooves are formed in two sides of the lengthening plate and the droplet generation plate seat, third sliding blocks are arranged in the third sliding grooves, and the third sliding blocks are fixedly connected with the inner wall of the PCR droplet generation amplification area.
Furthermore, a groove is formed in the top of the PCR data detector, and a PCR microdroplet generation amplification kit is arranged in the groove.
Furthermore, the second sliding block is arranged in a convex shape, and the bottom of the second sliding chute is communicated with the inside of the PCR microdroplet generation and amplification area.
Furthermore, the top of the droplet generation plate seat is provided with a support protrusion between every two adjacent droplet generation tubes, and the height of the support protrusion is greater than or equal to the height of the droplet generation tube on the top of the droplet generation plate seat.
Further, the droplet generation and amplification heating seat is made of a heat conducting material, the end of the droplet generation plate seat is made of a heat insulating material, and the outlet end of the PCR droplet generation and amplification area is matched with the combination of the droplet generation plate seat and the droplet generation pipe.
The utility model discloses a technological effect and advantage:
1. the utility model discloses an add the function that the droplet generated and amplified to PCR data detection appearance in, make three kinds of function an organic whole combine, the occupation space of how many equipment of effectual reduction to operate on integrative equipment, increase detection efficiency, improve the detection accuracy.
2. The utility model discloses a be equipped with the droplet and generate and expand and increase hot seat, be favorable to generating the droplet and expand and increase hot seat and droplet generator tube and wrap up the heating, increase the heating efficiency of droplet generator tube, improve the speed that the droplet generated and expanded, improve work efficiency.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by the practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims hereof as well as the appended drawings.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 shows a schematic overall structure diagram of an embodiment of the present invention;
fig. 2 shows a cross-sectional view of the overall structure of an embodiment of the present invention;
fig. 3 shows a schematic structural diagram of a portion a in fig. 1 according to an embodiment of the present invention;
FIG. 4 illustrates a top view of a droplet generation plate holder configuration according to an embodiment of the present invention;
figure 5 shows a left side view of a droplet generation plate mount and droplet generation amplification heating mount configuration in accordance with an embodiment of the present invention.
In the figure: 1. a PCR data detector; 2. PCR microdroplet generation of an amplification region; 3. a PCR data detection area; 4. a droplet generation plate holder; 5. a droplet-generating tube; 6. a sealing plate; 7. sealing the protrusion; 8. a sealing groove; 9. a permanent magnet; 10. pressing a plate; 11. a first slider; 12. a first chute; 13. droplet generation and expansion hot seats; 14. a heating resistor; 15. a second slider; 16. a second chute; 17. sealing the sliding plate; 18. sealing the nylon gasket; 19. a slot; 20. a spring; 21. a push rod; 22. pushing the groove; 23. an L-shaped slot; 24. a lengthening plate; 25. a third chute; 26. a third slider; 27. a groove; 28. PCR microdroplet generation amplification kit.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention are clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
The utility model provides a digital PCR droplet generates and amplifies and detects all-in-one as shown in figures 1-5, including PCR data detector 1, PCR data detector 1 includes that the PCR droplet generates and amplifies regional 2 and PCR data detection area 3, the PCR droplet generates and amplifies regional 2 inside and is the cavity setting, and the activity is pegged graft and is had droplet generation board seat 4, droplet generation board seat 4 surface activity runs through and pegs graft and has a plurality of droplet generation pipe 5, the one end top and the bottom of droplet generation board seat 4 are provided with sealing plate 6 and sealing bulge 7 respectively, sealing bulge 7 sets up with one section phase-match in droplet generation pipe 5 bottom, wherein sealing bulge 7's size is more than or equal to the size of the import position that the PCR droplet generated and amplified regional 2, PCR droplet generation amplifies regional 2 one end top and offers the seal groove 8 with sealing plate 6 phase-match, the permanent magnet 9 is arranged between the sealing plate 6 and the sealing groove 8, wherein the permanent magnet 9 is two same permanent magnets 9, the two permanent magnets 9 are arranged in a non-symmetrical manner, a pressing plate 10 is movably inserted on one side of the surface of the droplet generation plate seat 4, a first sliding block 11 is arranged between each of two sides of the pressing plate 10 and two ends of the droplet generation plate seat 4, first sliding grooves 12 matched with the first sliding blocks 11 are formed in two ends of the droplet generation plate seat 4, a droplet generation and amplification hot seat 13 is arranged at the bottom in the PCR droplet generation and amplification area 2, the droplet generation and amplification hot seat 13 is arranged to facilitate the coating and heating of the droplet generation and amplification hot seat 13 and the droplet generation pipe 5, the heating efficiency of the droplet generation pipe 5 is increased, the droplet generation and amplification speeds are increased, the working efficiency is improved, a heating resistor 14 is arranged in the droplet generation and amplification hot seat 13, wherein the heating resistor 14 is electrically connected with a power supply inside the PCR data detector 1 and is connected in series through a switch, a second slide block 15 is arranged at one end of the droplet generation and amplification hot seat 13 positioned inside the PCR droplet generation and amplification area 2, a second slide groove 16 matched with the second slide block 15 is arranged at one end inside the PCR droplet generation and amplification area 2, a sealing slide plate 17 is fixedly connected at the bottom of the other end of the droplet generation and amplification hot seat 13, a sealing nylon gasket 18 is embedded on the surface of the sealing slide plate 17, a slot 19 matched with the sealing slide plate 17 is arranged inside the PCR droplet generation and amplification area 2, a spring 20 is arranged between the bottom of the sealing slide plate 17 and the slot 19, two push rods 21 are inserted and connected on one side of the top of the sealing slide plate 17 in a sliding manner, and a push slot 22 matched with the push rods 21 is arranged on, the outer end of the PCR microdroplet generation and amplification area 2 is provided with an L-shaped groove 23 matched with the push rod 21, wherein the length of the L-shaped groove 23 is smaller than that of the sealing sliding plate 17, and the microdroplet generation and amplification functions are added into the PCR data detector 1, so that the three functions are integrated, the occupied space of the device is effectively reduced, the operation is performed on the integrated device, the detection efficiency is increased, and the detection accuracy is improved.
Further, in the above technical solution, an extension plate 24 is disposed at one end of the droplet generation plate seat 4 located inside the PCR droplet generation amplification area 2, third sliding grooves 25 are disposed at both sides of the extension plate 24 and the droplet generation plate seat 4, a third sliding block 26 is disposed inside the third sliding groove 25, the third sliding block 26 is fixedly connected to the inner wall of the PCR droplet generation amplification area 2, and the extension plate 24 is disposed to facilitate complete pulling out of the droplet generation plate seat 4, thereby facilitating operation and control of the pressing plate 10;
further, in the above technical solution, a groove 27 is formed at the top of the PCR data detector 1, and a PCR microdroplet generation amplification kit 28 is disposed inside the groove 27;
further, in the above technical solution, the second sliding block 15 is disposed in a convex shape, and the bottom of the second sliding chute 16 is communicated with the inside of the PCR microdroplet generation and amplification region 2, so that the microdroplet generation and amplification thermal seat 13 can be positioned while sliding;
further, in the above technical solution, supporting protrusions are disposed on the top of the droplet generation plate seat 4 between the adjacent four droplet generation tubes 5, and the height of the supporting protrusions is greater than or equal to the height of the droplet generation tubes 5 on the top of the droplet generation plate seat 4, which is beneficial to supporting the pressing plate 10 and damaging the droplet generation tubes 5;
further, in the above technical solution, the droplet generation and amplification thermal seat 13 is made of a heat conductive material, the end of the droplet generation plate seat 4 is made of a heat insulating material, and the outlet end of the PCR droplet generation and amplification area 2 is matched and arranged with the droplet generation plate seat 4 and the droplet generation pipe 5, so as to facilitate the operation of the heated droplet generation plate seat 4.
This practical theory of operation:
referring to the attached figures 1-5 of the specification, when the PCR technology is operated, the push rod 21 is manually pressed to move the push rod 21 downward in the L-shaped groove 23, after the push rod 21 moves to the bottom of the L-shaped groove 23, the push rod 21 is pushed to both sides to move the push rod 21 inside the push groove 22, so that the push rod 21 enters both sides of the bottom of the L-shaped groove 23, the droplet generation and amplification hot seat 13 is fixed under the elastic force of the spring 20, then the droplet generation plate seat 4 is pulled to separate the permanent magnet 9 at the sealing plate 6 and the sealing groove 8, the droplet generation plate seat 4 is pulled out, then the pressing plate 10 on the surface of the droplet generation plate seat 4 is pushed to slide the first slide block 11 inside the first sliding groove 12 to expose the inlet of the generation tube 5, then the reagent in the PCR droplet generation and amplification reagent kit 28 is added inside the generation tube 5 to push the droplet pressing plate 10 back to press the droplet generation tube 5, pushing the micro-droplet generation plate seat 4 into the PCR micro-droplet generation and amplification area 2, sealing the inlet position of the PCR micro-droplet generation and amplification area 2 through the sealing plate 6 and the sealing protrusion 7, then pushing the push rod 21 out from two sides of the bottom of the L-shaped groove 23, slowly pressing the push rod 21 to make the micro-droplet generation and amplification heat seat 13 move upwards slowly, making the bottom of the micro-droplet generation tube 5 enter the interior of the micro-droplet generation and amplification heat seat 13 for one section, sealing the position of the L-shaped groove 23 through the sealing sliding plate 17 and the sealing nylon pad 18, starting the heating resistor 14 to heat the micro-droplet generation tube 5 to make the internal reagent generate micro-droplets, then replacing the internal reagent in the same way to make the micro-droplets amplify, then adding the amplified reagent into the PCR data detection area 3 to detect, thereby effectively adding the functions of micro-droplet generation and amplification into the PCR data detector 1, make three kinds of functions an organic whole combine, the effectual occupation space who reduces how many equipment to operate on integrative equipment, increase detection efficiency, improve detection accuracy.
Although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.
Claims (6)
1. The utility model provides a digital PCR droplet generates amplification and detects all-in-one, includes PCR data detection appearance (1), its characterized in that: the PCR data detector (1) comprises a PCR microdroplet generation amplification area (2) and a PCR data detection area (3), wherein the PCR microdroplet generation amplification area (2) is arranged in a hollow mode, a microdroplet generation plate seat (4) is movably inserted, a plurality of microdroplet generation tubes (5) are movably inserted in the surface of the microdroplet generation plate seat (4) in a penetrating mode, a sealing plate (6) and a sealing bulge (7) are respectively arranged at the top and the bottom of one end of the microdroplet generation plate seat (4), the sealing bulge (7) is matched with one section of the bottom of the microdroplet generation tube (5) in a penetrating mode, a sealing groove (8) matched with the sealing plate (6) is formed in the top of one end of the PCR microdroplet generation amplification area (2), a permanent magnet (9) is arranged between the sealing plate (6) and the sealing groove (8), and a pressing plate (, first sliding blocks (11) are arranged between two sides of the pressing plate (10) and two ends of the droplet generation plate seat (4), first sliding grooves (12) matched with the first sliding blocks (11) are formed in the two ends of the droplet generation plate seat (4), a droplet generation and amplification heat seat (13) is arranged at the bottom in the PCR droplet generation and amplification area (2), a heating resistor (14) is arranged in the droplet generation and amplification heat seat (13), a second sliding block (15) is arranged at one end, located in the PCR droplet generation and amplification area (2), of the droplet generation and amplification heat seat (13), a second sliding groove (16) matched with the second sliding block (15) is formed at one end in the PCR droplet generation and amplification area (2), a sealing sliding plate (17) is fixedly connected to the bottom of the other end of the bottom of the droplet generation and amplification heat seat (13), and a sealing nylon cushion (18) is inlaid on the surface of the sealing sliding plate (17), the PCR micro-droplet generation amplification area (2) is internally provided with a slot (19) matched with the sealing sliding plate (17), a spring (20) is arranged between the bottom of the sealing sliding plate (17) and the slot (19), two push rods (21) are inserted into one side of the top of the sealing sliding plate (17) in a sliding manner, the surface of the sealing sliding plate (17) is provided with a push groove (22) matched with the push rods (21), and one end of the outer part of the PCR micro-droplet generation amplification area (2) is provided with an L-shaped groove (23) matched with the push rods (21).
2. The integrated digital PCR droplet generation, amplification and detection machine of claim 1, wherein: one end, located in the PCR microdroplet generation amplification area (2), of the microdroplet generation plate seat (4) is provided with an elongated plate (24), third sliding grooves (25) are formed in two sides of the elongated plate (24) and the microdroplet generation plate seat (4), third sliding blocks (26) are arranged in the third sliding grooves (25), and the third sliding blocks (26) are fixedly connected with the inner wall of the PCR microdroplet generation amplification area (2).
3. The integrated digital PCR droplet generation, amplification and detection machine of claim 1, wherein: the top of the PCR data detector (1) is provided with a groove (27), and a PCR microdroplet generation amplification kit (28) is arranged in the groove (27).
4. The integrated digital PCR droplet generation, amplification and detection machine of claim 1, wherein: the second slide block (15) is arranged in a convex shape, and the bottom of the second slide groove (16) is communicated with the inside of the PCR microdroplet generation and amplification area (2).
5. The integrated digital PCR droplet generation, amplification and detection machine of claim 1, wherein: supporting bulges are arranged on the tops of the droplet generation plate seats (4) between the adjacent four droplet generation pipes (5), and the height of each supporting bulge is larger than or equal to the height of the droplet generation pipe (5) on the tops of the droplet generation plate seats (4).
6. The integrated digital PCR droplet generation, amplification and detection machine of claim 1, wherein: the droplet generation and amplification thermal seat (13) is made of a heat conduction material, the end part of the droplet generation plate seat (4) is made of a heat insulation material, and the outlet end part of the PCR droplet generation and amplification area (2) is matched and arranged with the combination of the droplet generation plate seat (4) and the droplet generation pipe (5).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202021733568.1U CN213172376U (en) | 2020-08-19 | 2020-08-19 | Digital PCR microdroplet generation, amplification and detection integrated machine |
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| CN202021733568.1U CN213172376U (en) | 2020-08-19 | 2020-08-19 | Digital PCR microdroplet generation, amplification and detection integrated machine |
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| CN213172376U true CN213172376U (en) | 2021-05-11 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115181789A (en) * | 2022-09-14 | 2022-10-14 | 广州华银医学检验中心有限公司 | Single cell detection method based on full-automatic digital PCR all-in-one machine |
-
2020
- 2020-08-19 CN CN202021733568.1U patent/CN213172376U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115181789A (en) * | 2022-09-14 | 2022-10-14 | 广州华银医学检验中心有限公司 | Single cell detection method based on full-automatic digital PCR all-in-one machine |
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