CN213142059U - Brain slice incubation perfusion device - Google Patents
Brain slice incubation perfusion device Download PDFInfo
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- CN213142059U CN213142059U CN202021245838.4U CN202021245838U CN213142059U CN 213142059 U CN213142059 U CN 213142059U CN 202021245838 U CN202021245838 U CN 202021245838U CN 213142059 U CN213142059 U CN 213142059U
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- perfusion
- brain slice
- groove
- incubation
- slice incubation
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Abstract
The application relates to a brain slice incubation perfusion device, which comprises a perfusion main body and a support net; a perfusion groove is formed in the middle of the perfusion main body, and the support net is arranged in the groove and has a preset distance with the bottom of the groove; and a circulation hole is formed in one side of the perfusion main body and communicated with the perfusion groove. Can continuously supply cerebrospinal fluid to the perfusion groove, and the whole device has simple structure, is convenient to process and clean.
Description
Technical Field
The application relates to the technical field of biological test research, in particular to a brain slice incubation perfusion device.
Background
When an animal brain slice experiment is carried out, the brain slices need to be cultured, the cerebrospinal fluid needs to be continuously supplied to the brain slices, the brain slices are continuously supplied under the condition of ensuring the stability of the brain slices, and the circulation of the cerebrospinal fluid is preferably formed. There is currently no simple means to accomplish this.
SUMMERY OF THE UTILITY MODEL
In view of this, the present application provides a brain slice incubation perfusion device, which includes a perfusion main body and a support net;
a perfusion groove is formed in the middle of the perfusion main body, and the support net is arranged in the groove and has a preset distance with the bottom of the groove;
and a circulation hole is formed in one side of the perfusion main body and communicated with the perfusion groove.
The brain slice incubation perfusion device can be implemented by a brain slice incubation perfusion device, and further comprises a washer in a ring structure, wherein the washer is arranged below the supporting net.
As an embodiment of the brain slice incubation perfusion device, the gasket is placed at the bottom of the perfusion groove, and the gasket is made of an elastic material.
As an embodiment of the brain slice incubation perfusion device, the gasket and the support net are matched with the perfusion groove in size.
As an embodiment of the brain slice incubation perfusion device, the opening area of the upper end of the perfusion groove is larger than the area of the bottom of the perfusion groove.
As an embodiment of the brain slice incubation perfusion device, the cross section of the perfusion groove is circular, and the perfusion main body is of a rectangular structure.
As an embodiment of the brain slice incubation perfusion device, the perfusion main body is made of plastic.
As an embodiment of the brain slice incubation perfusion device, the perfusion body is 5 +/-0.3 cm in length and 4 +/-0.3 cm in width.
As an embodiment of the brain slice incubation perfusion device, the thickness of the perfusion main body is 2.8 +/-2 cm, and the flow hole is 0.2 cm.
As an embodiment of the brain slice incubation perfusion device, the supporting net is a flexible net, and the diameter of the mesh is 1 mm.
This scheme is through digging at perfusion main part headquarters and getting the perfusion recess, sets up the detachable supporting network at the recess middle part, and the lateral wall sets up the input channel of liquid to can fill into cerebrospinal fluid in the perfusion recess constantly, with cultivate the brain piece. The brain slice is placed on the supporting net, and the safety factor is higher. And the supporting net can be detached, so that the cleaning is convenient.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate exemplary embodiments, features, and aspects of the application and, together with the description, serve to explain the principles of the application.
Fig. 1 is a schematic structural diagram illustrating an embodiment of a brain slice incubation perfusion apparatus according to the present application;
fig. 2 shows a side view of a brain slice incubation perfusion apparatus of the present application;
fig. 3 shows a structural diagram of a gasket of a brain slice incubation perfusion apparatus according to the present application.
Detailed Description
Various exemplary embodiments, features and aspects of the present application will be described in detail below with reference to the accompanying drawings. In the drawings, like reference numbers can indicate functionally identical or similar elements. While the various aspects of the embodiments are presented in drawings, the drawings are not necessarily drawn to scale unless specifically indicated.
It should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientation or positional relationship indicated in the drawings for convenience in describing the invention or for simplicity in description, and do not indicate or imply that the device or element so indicated must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be considered as limiting the invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
The word "exemplary" is used exclusively herein to mean "serving as an example, embodiment, or illustration. Any embodiment described herein as "exemplary" is not necessarily to be construed as preferred or advantageous over other embodiments.
Furthermore, in the following detailed description, numerous specific details are set forth in order to provide a better understanding of the present application. It will be understood by those skilled in the art that the present application may be practiced without some of these specific details. In some instances, methods, means, elements and circuits that are well known to those skilled in the art have not been described in detail so as not to obscure the present application.
As shown in fig. 1, 2, and 3, a brain slice incubation perfusion device according to an embodiment of the present application includes a rectangular perfusion main body 100, a perfusion groove 200 is disposed in the middle of the perfusion main body 100, and a support net 300 is disposed in the perfusion groove 200. The support net 300 is used for supporting the brain slices placed inside the groove.
A flow hole 500 is formed at one side of the perfusion main body 100, the flow hole 500 is communicated with the perfusion groove 200, and in the process of brain slice cultivation, nutrient substances such as cerebrospinal fluid can be filled into the perfusion groove 200 through the flow hole 500, so that the groove can be filled with the nutrient substances, thereby performing brain slice incubation.
The device has a very simple integral structure and is convenient to process and produce. The utility model is also convenient for use in the brain slice incubation process. Moreover, the supporting net 300 can be a detachable structure with the Cao, which is convenient for cleaning.
In one embodiment, the brain supporting device further comprises a gasket 400, the gasket 400 is arranged below the supporting net 300, the supporting net 300 is directly placed above the gasket 400 and supported by the gasket 400, as shown in fig. 3, the middle part of the gasket 400 is of a hollow structure, and the supporting net 300 can sink down in the middle part within the elastic range, so that the brain slice can be well supported.
The specific gasket 400 is placed at the bottom of the perfusion groove 200, and the gasket 400 may also be made of an elastic material, so as to reduce the damage of the gasket 400 to the support net 300. As shown in fig. 1, the size of the gasket 400 and the supporting net 300 is matched with the size of the perfusion groove 200, the size of the periphery of the gasket 400 is consistent with the size of the bottom of the perfusion groove 200, and the gasket 400 is automatically fixed after being placed at the bottom of the perfusion groove 200. In practice, the gasket 400 may be fixed to the support net 300 by gluing, etc., while the gasket 400 is not fixed to the perfusion body 100, and the two are in clearance fit and can be freely detached. Therefore, the position of the supporting net 300 can be ensured to be stable, and the supporting net 300 communicated with the gasket 400 can be taken out at any time for cleaning, so that the use is convenient.
As shown in fig. 2, the perfusion groove 200 is a bowl-shaped structure, and the opening area of the upper end is larger than the area of the bottom. This design also facilitates subsequent removal of the gasket 400 and the support mesh 300, as well as cleaning of the entire apparatus. The cerebrospinal fluid can be conveniently and directly sucked out from the upper opening in the follow-up process, and the flow of the cerebrospinal fluid with stable temperature is ensured.
As shown in fig. 1, the cross section of the perfusion groove 200 is circular, and the perfusion main body 100 is rectangular, so that the perfusion groove is convenient to process and place.
For the material of perfusion main part 100, can choose the plastics material for use, middle part perfusion recess 200 can directly be detained, and the bottom is seal structure, and it is can not detain thoroughly.
For convenience of use, the perfusion main body 100 has a length of 5 + -0.3 cm, a width of 4 + -0.3 cm and a thickness of 2.8 + -2 cm. The size can also be adjusted according to actual requirements.
As shown in fig. 1, in this embodiment, the flow hole 500 disposed at the side of the perfusion main body 100 has a diameter of 0.2 cm, so as to continuously introduce cerebrospinal fluid into the perfusion recess 200.
Preferably, the support net 300 is a flexible net, and the diameter of the net is 1 mm.
Having described embodiments of the present application, the foregoing description is intended to be exemplary, not exhaustive, and not limited to the disclosed embodiments. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen in order to best explain the principles of the embodiments, the practical application, or improvements made to the technology in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.
Claims (10)
1. A brain slice incubation perfusion device is characterized by comprising a perfusion main body and a support net;
a perfusion groove is formed in the middle of the perfusion main body, and the support net is arranged in the groove and has a preset distance with the bottom of the groove;
and a circulation hole is formed in one side of the perfusion main body and communicated with the perfusion groove.
2. The brain slice incubation perfusion apparatus of claim 1, further comprising a washer in a ring configuration disposed below the support mesh.
3. The brain slice incubation perfusion apparatus of claim 2, wherein the gasket is placed at the bottom of the perfusion recess and the gasket is an elastic material.
4. The brain slice incubation perfusion apparatus of claim 3, wherein the gasket and the support mesh are sized to match the perfusion recess.
5. The brain slice incubation perfusion apparatus of claim 1, wherein the upper end opening area of the perfusion groove is larger than the bottom area.
6. The brain slice incubation perfusion apparatus according to claim 1, wherein the perfusion groove has a circular cross-section and the perfusion body has a rectangular structure.
7. The brain slice incubation perfusion apparatus of claim 1, wherein the perfusion body is made of plastic.
8. The brain slice incubation perfusion apparatus of claim 7, wherein the perfusion body is 5 ± 0.3 cm long and 4 ± 0.3 cm wide.
9. The brain slice incubation perfusion apparatus of claim 1, wherein the perfusion body has a thickness of 2.8 ± 2 cm and the flow hole is 0.2 cm.
10. The brain slice incubation perfusion apparatus of claim 1, wherein the support mesh is a flexible mesh with a mesh diameter of 1 mm.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202021245838.4U CN213142059U (en) | 2020-06-30 | 2020-06-30 | Brain slice incubation perfusion device |
Applications Claiming Priority (1)
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CN202021245838.4U CN213142059U (en) | 2020-06-30 | 2020-06-30 | Brain slice incubation perfusion device |
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CN213142059U true CN213142059U (en) | 2021-05-07 |
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CN202021245838.4U Active CN213142059U (en) | 2020-06-30 | 2020-06-30 | Brain slice incubation perfusion device |
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2020
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