JP2008067679A - Tank for rearing or culturing - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a tank for rearing or culturing capable of rearing or culturing an object, such as aquatic organisms or cells that require or prefers an environment that does not have liquid flow, at rearing or culturing. <P>SOLUTION: The tank for rearing or culturing has a top-surface opening inner tank, and an outer tank capable of holding the inner tank as a whole under fluid level. The tank for rearing or culturing is provided with a cylinder placed at the center of the inner tank, vertically penetrating the tank and having an upper end opening near the upper face of the inner tank, and a lower end opening at the bottom of the inner tank; an aeration means placed near the lower opening of the cylinder; and a flowing zone of the liquid formed around the outer circumference of the inner tank and connected to the cylinder. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention is a breeding / culturing tank for aquatic organisms, cells, etc., and particularly for breeding / cultivation used for breeding or culturing objects such as aquatic organisms, cells, etc. that require an environment free from liquid flow during breeding or culturing. Regarding the tank.

Conventionally, in the breeding or culturing of aquatic organisms and cells, as a method of supplying oxygen to the breeding solution or culture solution, in order to avoid damage to the aquatic organisms or cells, there is a method using only aeration without using mechanical agitation. There is known an apparatus for rearing or culturing using an air lift system (for example, see Patent Documents 1 and 2, etc.).
Even in the case of the air lift system, for example, in the breeding or culture of aquatic daphnia (aquatic micro organisms), if the water flow (liquid flow) is fast, a part of the organism is killed and the growth is inhibited. Therefore, in order to eliminate this obstacle, there is known a breeding / culturing apparatus equipped with a slow water flow generator that causes a gentle water flow while adopting an air lift system (for example, Patent Document 3) reference).

Patent Literature 1 JP 2005-261341 A Patent Literature 2 JP 7-67625 A Patent Literature 3 JP 2002-125509 A

  However, in the conventional breeding / culturing apparatus described above, it is difficult to breed or cultivate objects such as aquatic organisms and cells that require an environment in which no breeding fluid or culture fluid flows. That is, for example, in the case of breeding aquatic organisms such as a clione having a weak swimming ability, it may be swept away even by a weak liquid flow, and furthermore, bubbles due to this liquid flow or ventilation give stress, For example, in the case of culturing cells such as animal or plant embryos, there is a problem that the cells are deformed or necrotized by the shear force of the liquid flow.

  The present invention has been made in view of such problems of the prior art, and its object is to provide a suitable aquatic organism, cell, or the like that requires an environment free of liquid flow during breeding or culturing. The object is to provide a breeding / culturing tank capable of breeding or culturing an object.

As a result of intensive studies to achieve the above object, the present inventors
(1) The breeding / cultivation tank is made into a double tank provided with an inner tank having an upper surface opening for breeding or culturing the object inside and an outer tank for accommodating the entire inner tank below the liquid level. ,
(2) In the central part of the inner tank, a cylinder that penetrates vertically and has openings at both ends and has ventilation means in the vicinity of the lower end opening; and (3) Air lift action around the outer periphery of the inner tank. In the vicinity of the upper surface of the inner tank below the liquid level, a newly supplied oxygen-supplied liquid flowing out from the upper end opening of the cylinder is provided at the upper part of the inner tank. This oxygen-enriched liquid is not a liquid flow but is gradually diffused with the liquid in the inner tank where there is no liquid flow other than near the upper surface of the inner tank due to diffusion action. New knowledge such as being able to create an environment suitable for breeding or culturing in which there is no liquid flow in the inner tank by replacing it, etc. has been obtained, and the present invention has been completed based on these findings.

That is, the breeding / culturing tank of the present invention is a breeding / culturing tank comprising an inner tank with a bottom at the top opening and an outer tank that accommodates the entire inner tank below the liquid level,
A cylinder that is provided through the center of the inner tank vertically and has an upper end that opens near the upper surface of the inner tank, and a lower end that opens at the bottom of the inner tank,
Ventilation means provided in the vicinity of the lower end opening of the cylinder;
It is formed in the outer periphery of the said inner tank, and is provided with the distribution area of the said liquid connected to the said cylinder.

  In addition, a preferred embodiment of the breeding / culturing tank of the present invention is a liquid-permeable, permeable or cultivated object-preventing member that covers the upper surface opening of the inner tank and penetrates the cylinder up and down. The upper end opening of the cylinder is provided so as to be on the upper surface of the outflow prevention member or above.

  Furthermore, the other suitable form of the breeding / culturing tank of the present invention may be provided between the outer peripheral surface of the inner tank, which is a part of the flow area of the liquid, and the inner peripheral surface of the outer tank facing the outer peripheral surface. It is characterized in that it is provided with means for removing harmful substances in the formed distribution area.

  Still another preferred embodiment of the breeding / culturing tank of the present invention is characterized in that a heat exchange means for adjusting the liquid temperature is provided on the outer peripheral surface of the outer tank or in the flow area of the liquid.

  According to the present invention, an air lift system is adopted, and the oxygen that has risen and flowed out of the cylinder by the aeration means in the vicinity of the upper surface of the inner tank below the liquid surface where the object is bred or cultured. The supplied liquid is continuously and partially exchanged with the liquid in the upper part of the inner tank, and this oxygen-rich liquid is diffused and has no liquid flow other than in the vicinity of the upper surface of the inner tank. Provided with a breeding / culturing tank capable of breeding or culturing suitable aquatic organisms, cells, or other objects that require an environment free of liquid flow during breeding or culturing, etc. be able to.

  Hereinafter, the breeding / culture tank of the present invention will be described in detail.

The breeding / culturing tank of the present invention is used for breeding or culturing objects such as aquatic organisms and cells that require an environment free from liquid flow during breeding or culturing.
The object to be bred or cultured is not particularly limited, and examples thereof include clione, jellyfish, animal and plant embryo cells and organs, and the like.
In the present invention, “no liquid flow” means substantially no liquid flow including almost no liquid flow.

The breeding / culturing tank of the present invention is a double tank comprising an inner tank and an outer tank, and the object is bred or cultured in the inner tank.
The upper surface (upper end surface) of the inner tank is open at the entire surface and has a bottom, and the entire inner tank is accommodated under the liquid level in the outer tank. As described later, the reason for accommodating in this way is to smoothly generate a circulating flow in the circulation region of the liquid formed in the outer periphery of the inner tank in the outer tank by an air lift action. The depth from the liquid level to the upper surface of the inner tank is not particularly limited.
The shapes of the inner tank and the outer tank are not particularly limited, but the inner tank and the outer tank are preferably similar to each other, and those having a circular horizontal cross section are preferable.
The materials for the inner tank and the outer tank are not particularly limited, and for example, appropriate materials such as glass, a synthetic resin such as vinyl chloride resin and acrylic resin, and stainless steel are selected and used.

In the central part of the inner tank, a cylinder for an air lift having an appropriate diameter is provided so as to penetrate vertically, with the upper end of the cylinder opening near the upper surface of the inner tank, and the lower end of the inner tank. Open at the bottom.
Note that the position of the upper end opening of the cylinder is in the vicinity of the upper surface of the inner tank when the position is slightly above the liquid level in the outer tank above the upper surface of the inner tank. Is allowed to flow out in the overflow state, but further, at the position above it, the gas-liquid is separated at the upper part in the cylinder, and the outflow of the liquid is reduced or not. This is because the turbulent flow is generated in the inner tank at a position lower than the upper surface of the inner tank, making it difficult to create a target environment.
As the material of the cylinder, appropriate materials such as those similar to the inner tank and the outer tank are used.

The venting means provided in the vicinity of the lower end opening of the cylinder may be any means as long as the compressed air supplied from an external pressurized air supply source, for example, an air pump, an air compressor or the like, is bubbled. Are preferably used. Examples of the bubble generating member include nozzles, air stones, various spargers (perforated nozzles), and the like.
Further, the pressurized air is supplied to the bubble generating member through a supply pipe penetrating the bottom surface of the outer tub or a supply pipe inserted into the cylinder from above the cylinder. Is called.
The vicinity of the lower end opening of the cylinder, which is the attachment position of the ventilation means, refers to the lower part of the cylinder or the vicinity of the opening below the cylinder, and the generated bubbles can be guided into the cylinder. Any position is acceptable.

When the entire inner tub is placed and stored under the liquid level in the outer tub, the outer peripheral surface (outer surface and outer bottom surface) of the inner tub and the inner peripheral surface (inner side surface) of the outer tub facing the outer peripheral surface And an appropriate space is formed between the inner tank and the outer periphery of the inner tank, that is, an upper surface of the inner tank and an outer periphery (outward direction of the outer surface and the outer bottom surface). The
Further, this liquid circulation area communicates with the cylinder to form a liquid circulation area as a whole tank.

With the above configuration, the liquid is supplied (dissolved) with oxygen by the venting means, and the inside tank is raised from the upper end opening of the cylinder by raising the inside of the cylinder from below with air bubbles generated by ventilation (air lift action). The liquid in which the oxygen is newly supplied is continuously and partially replaced with the liquid in the upper part of the inner tank. Further, the liquid reaches the lower end opening of the cylinder in a downward flow and a horizontal flow from the upper end edge of the inner tank to the lower end opening of the cylinder, and thereafter the above state is continuously repeated. In the liquid circulation area, a circulation flow is generated.
Thus, in the vicinity of the upper surface of the inner tank, the liquid to which oxygen that has been partially but continuously replaced is newly supplied does not have a liquid flow other than the vicinity of the upper surface of the inner tank due to the diffusion action. The environment is gradually replaced with the liquid inside, and an environment suitable for breeding or culturing without a liquid flow is created.

In addition, the material of the cylinder is porous (for example, a porous synthetic resin (such as a pipe made of a vinyl chloride resin having many holes and a non-woven fabric made of a synthetic resin formed into a cylindrical shape), a fired ceramic, etc.) Various filter media (such as those made of synthetic resin filter media formed into a cylindrical shape), or slits (slits) that penetrate into the inner tank on the side surface of the cylinder but do not substantially generate a liquid flow Thus, there is an advantage that the liquid supplied with oxygen in the cylinder and the liquid in the inner tank are gradually replaced by a diffusion action.
In addition, a gas permeable material (for example, a silicone rubber tube or the like) is used as the material of the cylinder, or a gas permeable film (for example, silicon rubber) is provided in an opening portion that leads to the inner tank provided on the side surface of the cylinder. Or a tubular gas permeable membrane (for example, made of silicon rubber) is placed at the bottom of the inner tank, and oxygen is fed from the outside to the rearing or More oxygen can be supplied to the culture environment.
For the same purpose as in the case of the cylinder, a rigid porous or gas permeable material is used as the material of the inner tank, and slits are provided on the peripheral surface (side surface and bottom surface) of the inner tank. Furthermore, a gas permeable membrane can be attached to the opening provided on the peripheral surface.

  In the breeding / cultivation tank, the method for supporting the inner tank in the outer tank is not particularly limited as long as the circulation flow of the liquid is ensured. For example, the outer tank and the inner tank The inner tank can be removed at a fixed position in the outer tank by a support member that can be attached to and detached from the inner tank (for example, a mounting table) or a support member fixed to the outer peripheral surface of the inner tank or the inner peripheral surface of the outer tank. Arranged.

The upper surface of the outer tub may be covered with a cover if necessary, and the cover may be provided with a vent or a bactericidal filter.
In addition, the breeding / culturing tank is provided with appropriate means for supplying and discharging the liquid. In this case, the liquid supply port and the discharge port are provided at appropriate positions where no liquid flow occurs in the inner tank.
Furthermore, when aseptically cultivating objects such as cells, a sterilized breeding / culturing tank is used, as well as air and liquid supply and discharge systems, and aseptic conditions have been secured by conventional methods. Things are used.
Furthermore, when the breeding / cultivation tank of the present invention is used for feeding breeding or feeding culture, an appropriate feed supply means is provided for feeding the feed into the environmental area where there is no liquid flow in the inner tank when necessary.

Next, in the breeding / culturing tank of the present invention, since the upper surface of the inner tank is open as described above, a part of the object to be reared or cultured flows out of the inner tank, or May contact the foam in the vicinity of the upper surface of the inner tank and damage the object.
In such a case, it is preferable that the upper surface opening of the inner tank is covered (partitioned) with a liquid-proof, outflow prevention member for an object to be bred or cultured.
At this time, the outflow prevention member is provided such that the cylinder penetrates vertically and the upper end opening of the cylinder is at the upper surface of the outflow prevention member or above.

  With this configuration, the liquid that has flowed out from the upper end opening of the cylinder to the upper surface or upward of the outflow prevention member is continuously and partially replaced with the liquid in the inner tank in the vicinity of the outflow prevention member. In addition, the outflow of the object to be bred or cultured in the inner tank to the outside of the inner tank is prevented, and further, the object is prevented from coming into contact with bubbles contained in the liquid flowing out from the upper end opening of the cylinder. Can do.

The outflow prevention member of the object to be reared or cultured is liquid-permeable, that is, a sheet (thin plate) having an appropriate thickness having the property that the liquid can freely pass through. Examples thereof include a foamed resin (for example, polyurethane foam), a porous body such as a nonwoven fabric, and a fine mesh mesh.
In addition, about the attachment method of the said outflow prevention member, what is necessary is just to employ | adopt an appropriate method, for example, the method of fixing to the said cylinder with an adhesive agent etc., and fixing to the upper surface periphery or inner wall of the said inner tank with an adhesive agent or a support member And a method of detachably fitting to the inner wall of the inner tank or the upper part of the cylinder.
Further, when the outflow prevention member is placed on the upper end edge surface of the inner tank and covers the upper surface opening of the inner tank, the peripheral edge of the outflow prevention member is extended to contact the inner peripheral surface of the outer tank. It may be provided as follows.

Furthermore, when raising or cultivating an object using the breeding / culturing tank of the present invention, ammonia, organic acids and other metabolites (water-soluble harmful substances), solid contaminants (excrements, food residues, etc.) The entire liquid including the liquid in the breeding or culturing environment in the inner tank may be gradually contaminated by harmful substances such as wastes.
In the first place, since the breeding / culturing tank of the present invention is used in addition to the amount of liquid in the inner tank in which the object is raised or cultured, the amount of liquid for generating a circulating flow is conventionally used. Compared to this breeding / culturing apparatus, a large amount of liquid is used, and the concentration of harmful substances is diluted.
As one of the embodiments of the breeding / culturing tank of the present invention that further improves the above, an outer peripheral surface of the inner tank that is a part of the circulation region of the liquid and an inner periphery of the outer tank facing the outer peripheral surface It is preferable that a means for removing harmful substances is provided in a portion of a liquid circulation area (a downward flow portion and a horizontal flow portion of the circulation flow) formed between the surfaces.

Usually, when there is no liquid flow like the liquid in the inner tank, it is inefficient or difficult to remove harmful substances using the removing means.
However, in the breeding and culturing tank of the present invention, the contaminated liquid in the inner tank that is gradually replaced is included in the circulating flow of the liquid generated by the air lift action. If the circulating fluid is processed by means of removing harmful substances, harmful substances can be continuously removed from the fluid, so that the entire system can be purified as well as the environment of breeding or culture. It is very effective.

The means for removing the harmful substance is not particularly limited, and a means for removing the harmful substance that is included in the liquid during breeding or culturing of the target object is appropriately employed.
For example, in the case of water-soluble harmful substances such as ammonia and organic acids, physical or chemical adsorption by activated carbon or zeolite, immobilized microorganisms (supported on an insoluble carrier or confined in the internal space) If the removal means such as assimilation by the microorganisms is a solid contaminant, a removal means such as physical filtration is used.

The method for installing the removing means for the harmful substances is not particularly limited. For example, the removing means (for example, a part of the circulation region of the liquid that passes through a part or all of the liquid in the circulation region) For example, when the shape of the inner tub and the outer tub is a circular shape in the horizontal section, a donut-shaped shape or the like may be provided via an appropriate support member.
In addition, the outflow prevention member for the object to be bred or cultured may have the same function as the means for removing the water-soluble harmful substance.

Furthermore, as another embodiment of the breeding / culturing tank of the present invention, it is preferable that a heat exchanging means for adjusting the liquid temperature is provided on the outer peripheral surface of the outer tank or the flow area of the liquid.
Generally, when adjusting the temperature of a liquid at the time of breeding or culturing an object to a certain range, a method of directly circulating or stirring the liquid to be bred or cultured is employed.
However, these methods cannot be employed when adjusting the liquid temperature in an environment region where there is no liquid flow as in the present invention.
In the breeding / culturing tank of the present invention, since there is a circulating flow in the circulation region of the liquid in the outer tank, the liquid temperature in the inner tank without the liquid flow can be adjusted by adjusting the liquid temperature of the circulating flow. It can be easily maintained within a certain range.

When the heat exchange means is provided on the outer peripheral surface (outer surface and outer bottom surface) of the outer tub, various heaters, coolers, etc. that are in contact with the outer peripheral surface are also provided in the liquid circulation area. These include a heat source such as a throwing heater and a cooling source such as a cooling water circulation pipe.
The liquid temperature of the circulating flow is adjusted through the outer wall or directly by the heat exchange means controlled by a signal from the circulating flow or the liquid temperature sensor in the inner tank. The liquid temperature inside is maintained within a certain range.

  Moreover, when breeding or cultivating cold water aquatic organisms, for example, clione or the like for ornamental use, dew condensation on the outer peripheral surface of the outer tank in the breeding / culturing tank becomes a problem. In such a case, the whole breeding / culturing tank is placed in a sealed space section partitioned by, for example, an outside air shielding means (the ornamental surface is made of a transparent hollow body in which a gas such as air is enclosed). The dew condensation can be prevented by housing at least the upper and side surfaces of the outside air shielding means in a non-contact state.

  Hereinafter, the present invention will be described in more detail with reference to the drawings, but the present invention is not limited to these examples.

(Example 1)
FIG. 1 is a schematic cross-sectional view and a plan view showing an embodiment of the breeding / culturing tank according to the present invention. This plan view is obtained when the cover of the outer tank is removed.
In the figure, a breeding / culturing tank 1 has an inner tank 2 (for example, made of transparent vinyl chloride resin) having an open top surface and a circular horizontal section, and an outer tank 3 (for example, transparent chloride) having a circular horizontal section. It is installed in a vinyl resin) via a support member (not shown), and is accommodated so that the entire inner tank 2 is below the liquid level.

At the center of the inner tub 2, a cylinder 4 (for example, made of transparent vinyl chloride resin) penetrating vertically is provided, and the upper end of the cylinder 4 is slightly lower than the upper surface of the inner tub 2 (near the upper surface). Opened, and the lower end is opened at the bottom surface of the inner tank 2.
Further, a ventilation means 5 such as a sparger is provided in the vicinity of the lower end opening of the cylinder 4, and the ventilation means 5 is provided via an air supply pipe 6 and, if necessary, a sterilization filter or the like. , Connected to a pressurized air supply source (not shown) such as an air compressor.

The inner tub 2 is detachably installed in the outer tub 3. In this installation, the outer peripheral surface (outer surface and outer bottom surface) of the inner tub 2 and the inner peripheral surface (inner An appropriate space between the inner tub 2 and the outer periphery of the inner tub 2, that is, the upper and outer circumferences of the inner tub 2 (outward direction of the outer and outer bottom surfaces). Is formed.
Further, this liquid circulation area communicates with the cylinder 4 to form a liquid circulation area as a whole tank, and in this liquid circulation area, a liquid circulation flow is generated by an air lift action.
In addition, 3a is a cover body which covers the upper surface of the outer tub 3, and an air vent, a bactericidal filter, etc. (not shown) are provided in this as needed.

The usage example of the breeding / culturing tank 1 of the present embodiment will be described.
First, the liquid is filled in the outer tub 3 so that the entire inner tub 2 is below the liquid level.
Next, an object to be bred or cultured is accommodated in the inner tank 2.
When breeding or culturing is started, as indicated by arrows in FIG. 1, oxygen is supplied (dissolved) to the liquid by the aeration means 5, and the liquid rises from below in the cylinder 4 by bubbles generated by aeration ( Air lift action) flows out from the upper end opening of the cylinder 4 into the liquid in the circulation area near the upper surface of the inner tank 2, and in the vicinity, the liquid to which oxygen is newly supplied is the liquid in the upper part of the inner tank 2. And continuously and partially. Further, the liquid descends from the upper end edge of the inner tank 2 through the liquid circulation area on the outer periphery of the inner tank 2 to become a horizontal flow and reaches the lower end opening of the cylinder 2, and then the above state is continuously repeated. In the liquid circulation area, a circulation flow is generated.

  In this way, the liquid newly supplied with oxygen that has been partially but continuously replaced in the vicinity of the upper surface of the inner tank 2 has no liquid flow other than in the vicinity of the upper surface of the inner tank 2 due to the diffusion action. The environment in the tank 2 is gradually changed to create a suitable environment for breeding or culturing without liquid flow, and the object in the tank 2 is normally bred or cultured.

(Example 2)
FIG. 2 is a schematic sectional view showing another embodiment of the breeding / culturing tank of the present invention.
In the following embodiments, the same reference numerals are given to substantially the same members and places as in the above embodiments, and the description thereof is omitted.
The breeding / culture tank 11 shown in FIG. 2 includes the following liquid-permeable, outflow prevention member 7 for the object to be bred or cultured, and the upper end of the cylinder 14 is slightly higher than the upper surface of the inner tank 2. Except for opening at a high position (near the upper surface), it has the same configuration as described in Example 1.

The outflow prevention member 7 is a liquid-permeable thin plate-like sponge-like porous body (eg, polyurethane foam), and is provided so as to be placed on the upper end edge surface of the inner tank 2 and cover the entire upper surface opening. ing.
The outflow prevention member 7 is attached by fitting a cylinder 14 into a hole penetrating vertically at the center thereof, and the upper end opening of the cylinder 14 is slightly larger than the upper surface of the outflow prevention member 7. Located high.

Next, a usage example of the breeding / culturing tank 11 of the present embodiment will be shown.
When breeding or culturing is started in the same manner as described in Example 1, the liquid newly supplied with oxygen flowing out from the upper end opening of the cylinder 14 is liquid-permeable and thin plate-like outflow prevention around it. Through the member 7, the liquid in the upper part of the inner tub 2 is continuously and partially replaced. At this time, the outflow prevention member 7 contacts the bubbles contained in the liquid flowing out from the upper end opening of the cylinder 14 when the object to be bred or cultured in the inner tank 2 flows out of the inner tank 2. Thereafter, a liquid circulation flow is generated in the same manner as described in the first embodiment, and in this way, in the vicinity of the upper surface of the inner tank 2, although partially, The liquid to which oxygen that has been continuously replaced is newly supplied is gradually replaced with the liquid in the inner tank 2 that does not have a liquid flow other than the vicinity of the upper surface of the inner tank 2 due to the diffusion action. A suitable environment is created and the object in the inner tank 2 is normally raised or cultured.

(Example 3)
FIG. 3 is a schematic sectional view showing another embodiment of the breeding / culturing tank of the present invention.
The breeding / culturing tank 21 shown in FIG. 3 is used for breeding a clione which is a kind of aquatic aquatic organisms and for ornamental purposes. The harmful substance removing means 8 and heat described below are used. It has the same configuration as described in Example 2 except that it includes the exchange means 9 and the lid is not covered, and this breeding / culture tank 21 will be described later. Condensation prevention measures are taken.

The harmful substance removing means 8 is provided in the liquid circulation area formed by the outer bottom surface of the inner tank 2 and its extended surface and the inner bottom surface of the outer tank 3 which are part of the liquid circulation area. It has a donut shape having a hollow portion having a diameter larger than that of the tube 14 at the center. A filter medium (eg, glass fiber) is used for the harmful substance removing means 8.
An electronic cooling device (thermo cooler) is provided in contact with the outer bottom surface of the outer tub 3 as the heat exchange means 9 controlled by a signal from a circulating liquid temperature sensor (not shown). The liquid temperature of the circulating flow is adjusted through the wall.
In addition, instead of the polyurethane foam illustrated in Example 2, the spillage prevention member 7 for the object to be bred or cultivated does not flow out of clione but floats excrement (solid content) passes and flows out. Is used.

Further, the breeding / culture tank 21 is housed in the substantially sealed space 51 formed by the outside air shielding means 50 in order to prevent condensation.
The outside air shielding means 50 includes a hollow side wall partition member 50b (eg, made of transparent vinyl chloride resin) having a transparent and cylindrical double wall, and an upper end portion of the side wall partition member 50b formed near the periphery. A disc-shaped upper surface partition member 50a (for example, made of transparent or opaque vinyl chloride resin) to be fitted in the groove, and a donut shape to be fitted in a groove in which the lower end portion of the side surface partition member 50b is formed near the periphery. (Round-shaped) lower surface partition member 50c (example: made of transparent or opaque vinyl chloride resin) is composed of three partition members, and the bottom of the outer tub 3 is inserted into the hollow portion of the donut-shaped lower surface partition member 50c. ing. At this time, the breeding / culture tank 21 is accommodated in a non-contact state on the inner surfaces of the upper surface partition member 50a and the side surface partition member 50b of the outside air shielding means 50.
Air is sealed in the hollow body of the side surface partition member 50b.
In addition, an air vent 52 is provided in the center of the upper surface partition member 50a.

Next, a usage example of the breeding / culture tank 21 of the present embodiment will be shown.
First, the liquid (seawater) in the breeding / culture tank 21 is cooled and maintained at 2 to 5 ° C. in advance by the heat exchange means 9 while ventilating, and then the upper partition member 50a is removed and the inner tank 2 is removed. After putting a clione (not shown) in the inside and attaching the upper surface partition member 50a, the breeding was carried out while ventilating, and the clione was observed and observed at a room temperature of 23 to 25 ° C.
The clione with weak swimming ability maintained normal habitat while showing slight movement (floating) in the inner tank 2 where there was no liquid flow.

Further, some excrement floating near the lower surface of the outflow prevention member 7 of the object to be bred or cultured passes through the outflow prevention member and is removed by the filter medium of the harmful substance removing means 8, and further reared. As a result, microorganisms capable of assimilating ammonia settled on this filter medium, and the metabolite ammonia was removed, and a good breeding environment was maintained.
Insulating action of the two gas layers of the gas layer (saturated water vapor at a low temperature substantially the same as the liquid temperature) in the space 51 of the outside air shielding means 50 and the gas (air) layer in the hollow body of the side partition member 50b. As a result, condensation on the outer surface of the outer tub 3 which is an observation and appreciation surface was prevented, and the clione in the breeding / culture tank 21 could be observed and appreciated in a good state.

These are the general | schematic cross section and top view which show one Example of the breeding and culture tank of this invention. These are the schematic sectional drawings which show the other Example of the breeding and culture tank of this invention. These are schematic sectional drawings which show still another embodiment of the breeding / culturing tank of the present invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1, 11, 21 Breeding / culture tank 2 Inner tank 3 Outer tank 4, 14 Cylinder 5 Ventilation means 7 Outflow prevention member 8 Hazardous substance removal means 9 Heat exchange means 50 Outside air shielding means 51 Space part

Claims (4)

A breeding / culturing tank comprising an inner tank with a bottom at the top opening and an outer tank that accommodates the entire inner tank below the liquid level,
A cylinder that is provided through the center of the inner tank vertically and has an upper end that opens near the upper surface of the inner tank, and a lower end that opens at the bottom of the inner tank,
Ventilation means provided in the vicinity of the lower end opening of the cylinder;
A breeding / culturing tank, comprising: an outer periphery of the inner tank; and a circulation area of the liquid communicating with the cylinder. Covering the upper surface opening of the inner tub and allowing the tube to penetrate vertically, the permeable outflow prevention member of the object to be bred or cultured, the upper end opening of the tube is the upper surface of the outflow prevention member or from it The breeding / culturing tank according to claim 1, wherein the breeding / culturing tank is provided so as to face upward. A means for removing harmful substances is provided at a portion of the liquid circulation area formed between the outer peripheral surface of the inner tank, which is a part of the liquid circulation area, and the inner peripheral surface of the outer tank facing the outer peripheral surface. The breeding / culturing tank according to claim 2 or 3, wherein the breeding / culturing tank is provided. The breeding / culturing tank according to any one of claims 1 to 3, further comprising a heat exchanging means for adjusting a liquid temperature in an outer peripheral surface of the outer tank or in a circulation region of the liquid.

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