CN213141988U

CN213141988U - Erythrocyte lysis kit

Info

Publication number: CN213141988U
Application number: CN202021425863.0U
Authority: CN
Inventors: 童艳铮
Original assignee: Nanjing Meiaohongqing Biotechnology Co ltd
Current assignee: Nanjing Meiaohongqing Biotechnology Co ltd
Priority date: 2020-07-17
Filing date: 2020-07-17
Publication date: 2021-05-07
Anticipated expiration: 2030-07-17

Abstract

The utility model relates to a reagent box for red blood cell lysis, which comprises a box body, a box cover integrated with the box body and a reagent bottle fixed in the box body; be equipped with the elastic cushion that is separated by the division board in the box body and place the frame, it places the district to be equipped with at least one schizolysis reagent in the elastic cushion, at least one washing reagent places the district and at least one fixed reagent places the district, the reagent bottle is fixed in respectively correspond in the elastic cushion place in the district the utility model discloses simple structure, convenient to use, effect are stable, are favorable to the standardization of erythrocyte lysis experiment. The utility model discloses be equipped with the elastic cushion and place the frame, the reagent bottle can be fixed better to the elastic cushion, avoids rocking and damaging of reagent bottle in the transportation, places the frame and can make things convenient for drawing and placing of reagent in the experimentation, places the frame simultaneously and can break away from the kit and place, avoids depositing the reagent in the experimentation to the elastic cushion and causes the influence, provides the guarantee for the standardization of this experimental operation of red blood cell schizolysis.

Description

Erythrocyte lysis kit

Technical Field

The utility model relates to a biomedical field especially relates to an erythrocyte lysis kit.

Background

The erythrocyte lysis method adopts lysis solution to lyse erythrocytes, does not damage nucleated cells, can fully remove the erythrocytes, and is a mild method which is most convenient and feasible for removing the erythrocytes. The erythrocyte lysis method can be used for separating and purifying histocytes dispersed by enzyme digestion, separating and purifying lymphocytes, and removing erythrocytes in experiments such as extraction of histocyte proteins and nucleic acids. The tissue cells obtained by the lysis of the erythrocyte lysate do not contain erythrocytes, and can be further used for primary culture, cell fusion, flow cytometry analysis, separation and extraction of nucleic acid and protein, and the like. Therefore, erythrocyte lysis is a commonly used technical means in the field of molecular cell biology.

The erythrocyte lysis needs to use an erythrocyte lysis reagent, and the selection of the erythrocyte lysis reagent and the experimental operation of the erythrocyte lysis have great influence on the detection result. Therefore, it is highly desirable to provide a reagent kit for lysing erythrocytes, which is easy to handle and has stable effect.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing an erythrocyte lysis kit aiming at the defects in the prior art.

In order to achieve the purpose, the utility model adopts the technical proposal that:

providing an erythrocyte lysis kit, which comprises a box body, a box cover connected with the box body into a whole and a reagent bottle fixed in the box body; the reagent bottle comprises a box body, wherein an elastic soft cushion and a placing frame are arranged in the box body, the elastic soft cushion is separated by a partition plate, at least one cracking reagent placing area, at least one washing reagent placing area and at least one fixed reagent placing area are arranged in the elastic soft cushion, and the reagent bottles are respectively fixed in the elastic soft cushion correspondingly in the placing areas.

Preferably, the elastic soft cushion is one or more composite material plates of a sponge plate, a foam plate, a soft plastic plate or a rubber plate.

Preferably, at least one first reagent groove, at least one second reagent groove and at least one third reagent groove are respectively and correspondingly arranged in the placing rack, and the diameter of each reagent groove is equal to or larger than that of each reagent bottle.

Preferably, the portable electronic device further comprises a first magnetic block and a second magnetic block which have magnetic attraction between each other, the first magnetic block is arranged on the box cover, the second magnetic block is arranged on the corresponding position of the box body, and when the box cover is in contact with the box body, the first magnetic block and the second magnetic block are connected through magnetic attraction.

Preferably, the reagent bottle is provided with scale marks.

The utility model adopts the above technical scheme, compare with prior art, have following technological effect:

the utility model discloses simple structure, convenient to use, effect are stable, are favorable to the standardization of erythrocyte lysis experiment. The utility model discloses be equipped with the elastic cushion and place the frame, the reagent bottle can be fixed better to the elastic cushion, avoids rocking and damaging of reagent bottle in the transportation, places the frame and can make things convenient for drawing and placing of reagent in the experimentation, places the frame simultaneously and can break away from the kit and place, avoids depositing the reagent in the experimentation to the elastic cushion and causes the influence, provides the guarantee for the standardization of this experimental operation of red blood cell schizolysis.

Drawings

FIG. 1 is a schematic structural diagram of an erythrocyte lysis kit according to the present invention;

wherein the reference numerals are: a box body 1; an elastic cushion 11; a lysis reagent placement zone 111; a washing reagent placement zone 112; an immobilized reagent placing zone 113; a placement frame 12; a first reagent tank 121; a second reagent tank 122; a third reagent tank 123; a partition plate 13; a second magnetic block 14; a box cover 2; a first magnetic block 21; and a reagent bottle 3.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.

It should be noted that, in the present invention, the embodiments and features of the embodiments may be combined with each other without conflict.

The present invention will be further described with reference to the accompanying drawings and specific embodiments, but the present invention is not limited thereto.

Examples

The embodiment provides an erythrocyte lysis kit, which comprises a box body 1, a box cover 2 connected with the box body 1 into a whole, and a reagent bottle 3 fixed in the box body 1; the reagent bottle box comprises a box body 1 and is characterized in that an elastic soft cushion 11 and a placing frame 12 which are separated by a partition plate 13 are arranged in the box body 1, at least one cracking reagent placing area 111, at least one washing reagent placing area 112 and at least one fixed reagent placing area 113 are arranged in the elastic soft cushion 11, and the reagent bottles 3 are respectively fixed in the corresponding placing areas in the elastic soft cushion 11.

Further preferably, the box body 1 is a cuboid, a cube or a cylinder.

In a preferred embodiment, the elastic cushion 11 is one or more composite material plates selected from a sponge plate, a foam plate, a soft plastic plate, and a rubber plate, and can not only fix the reagent bottle 3, but also cushion and protect the reagent bottle during movement.

In a preferred embodiment, at least one first reagent tank 121, at least one second reagent tank 122 and at least one third reagent tank 123 are respectively and correspondingly arranged in the rack 12, the diameter of the reagent tank is equal to or greater than the diameter of the reagent bottle 3, the rack 12 is a hollow structure, the volume of the solution in the reagent bottle on the rack can be clearly seen, and the rack 12 is movable, so that the placement of the reagent box can be separated, and the influence on the reagent stored in the elastic cushion 11 in the experiment process can be avoided.

In a preferred embodiment, the portable electronic device further includes a first magnetic block 21 and a second magnetic block 14 having a magnetic attraction force therebetween, the first magnetic block 21 is disposed on the lid 2, the second magnetic block 14 is disposed at a corresponding position of the case 1, and when the lid 2 contacts the case 1, the first magnetic block 21 and the second magnetic block 14 are attracted and connected by the magnetic attraction force.

As a preferable embodiment, the reagent bottle 3 is provided with scale marks, and the material is a medical transparent material, so that a more objective judgment basis is provided for the definition of the red blood cells after the red blood cells are cracked, and the standardization of a red blood cell cracking experiment is facilitated.

To sum up, the utility model provides a pair of erythrocyte lysis kit has simple structure, convenient to use, stable advantage of effect, is favorable to the standardization of erythrocyte lysis experiment. The utility model discloses be equipped with the elastic cushion and place the frame, the reagent bottle can be fixed better to the elastic cushion, avoids rocking and damaging of reagent bottle in the transportation, places the frame and can make things convenient for drawing and placing of reagent in the experimentation, places the frame simultaneously and can break away from the kit and place, avoids depositing the reagent in the experimentation to the elastic cushion and causes the influence, provides the guarantee for the standardization of this experimental operation of red blood cell schizolysis.

The above description is only an example of the preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and those skilled in the art should be able to realize the equivalent alternatives and obvious variations of the present invention.

Claims

1. The erythrocyte lysis kit is characterized by comprising a box body (1), a box cover (2) which is connected with the box body (1) into a whole, and a reagent bottle (3) which is fixed in the box body (1); be equipped with in box body (1) by elastic cushion (11) and the shelf (12) that division board (13) separated, be equipped with at least one schizolysis reagent in elastic cushion (11) and place district (111), at least one washing reagent and place district (112) and at least one fixed reagent and place district (113), reagent bottle (3) are fixed in respectively in elastic cushion (11) corresponding place in the district.

2. The erythrocyte lysis kit according to claim 1, wherein the flexible cushion (11) is one of a sponge sheet, a foam sheet, a soft plastic sheet, or a rubber sheet.

3. The kit for red blood cell lysis according to claim 1, wherein at least one first reagent well (121), at least one second reagent well (122) and at least one third reagent well (123) having a diameter equal to or greater than the diameter of the reagent bottle (3) are respectively provided in the rack (12).

4. The erythrocyte lysis kit according to claim 1, further comprising a first magnetic block (21) and a second magnetic block (14) having a magnetic attraction force therebetween, wherein the first magnetic block (21) is disposed on the lid (2), and the second magnetic block (14) is disposed at a corresponding position on the case body (1), and when the lid (2) is in contact with the case body (1), the first magnetic block (21) and the second magnetic block (14) are connected by the magnetic attraction force.

5. The erythrocyte lysis kit according to claim 1, wherein the reagent bottle (3) is provided with graduation marks.