CN209979547U - Horizontal agarose electrophoresis device for DNA detection and gel maker thereof - Google Patents
Horizontal agarose electrophoresis device for DNA detection and gel maker thereof Download PDFInfo
- Publication number
- CN209979547U CN209979547U CN201822184678.6U CN201822184678U CN209979547U CN 209979547 U CN209979547 U CN 209979547U CN 201822184678 U CN201822184678 U CN 201822184678U CN 209979547 U CN209979547 U CN 209979547U
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- gel
- agarose electrophoresis
- tank
- horizontal
- cell body
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- 238000001962 electrophoresis Methods 0.000 title claims abstract description 38
- 229920000936 Agarose Polymers 0.000 title claims abstract description 22
- 238000001514 detection method Methods 0.000 title claims description 19
- 210000005056 cell body Anatomy 0.000 claims abstract description 10
- 230000006978 adaptation Effects 0.000 claims abstract description 3
- 239000000499 gel Substances 0.000 claims description 30
- 239000011543 agarose gel Substances 0.000 claims description 8
- 238000009423 ventilation Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 7
- 239000000463 material Substances 0.000 abstract description 5
- 239000007788 liquid Substances 0.000 abstract description 4
- 229920000515 polycarbonate Polymers 0.000 abstract description 3
- 239000004417 polycarbonate Substances 0.000 abstract description 3
- 230000002349 favourable effect Effects 0.000 abstract description 2
- 238000001746 injection moulding Methods 0.000 abstract description 2
- 238000004026 adhesive bonding Methods 0.000 abstract 1
- 239000003292 glue Substances 0.000 description 13
- 238000002474 experimental method Methods 0.000 description 9
- 239000000243 solution Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241001391944 Commicarpus scandens Species 0.000 description 1
- -1 DNA Chemical class 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 210000001520 comb Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model discloses a horizontal agarose electrophoresis apparatus for DNA detects and system gluey ware thereof, wherein, a horizontal agarose electrophoresis apparatus for DNA detects, including cell body, capping and positive and negative electrode frame, the cell body with capping looks adaptation connection, cell body diagonal both ends are equipped with the electrode tank respectively, positive and negative electrode frame sets up respectively in the electrode tank. Therefore, the utility model provides a horizontal agarose electrophoresis apparatus for DNA detects and system ware of gluing thereof, cell body adopt high strength high transparency polycarbonate material injection moulding, but a groove is multi-purpose, avoids the liquid seepage, is favorable to observing the electrophoresis process, and multiple safety design has strengthened the safety of operation.
Description
Technical Field
The utility model relates to a gene detection and molecular detection technical field especially relate to a horizontal agarose electrophoresis apparatus for DNA detects and system ware is glued.
Background
Agarose electrophoresis is a simple, rapid method for separating, purifying and identifying nucleic acids, particularly DNA, using agar or agarose as the support for electrophoresis. For samples with larger molecular weight, such as macromolecular nucleic acids, viruses, etc., agarose gel with larger pore size is generally used for electrophoretic separation. The existing electrophoresis device has poor mechanical strength, is easy to break, is easy to be polluted by bacteria, is not easy to store, is easy to leak liquid during glue making, is not beneficial to electrophoresis operation, and reduces the safety of experiment operation.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a horizontal agarose electrophoresis apparatus for DNA detects and system ware is glued to it.
The utility model provides a horizontal agarose electrophoresis apparatus for DNA detects, including cell body (1), capping (2) and positive negative electrode frame (3), cell body (1) with capping (2) looks adaptation is connected, cell body (1) diagonal both ends are equipped with electrode tank (4) respectively, positive negative electrode frame (3) set up respectively in electrode tank (4).
The agarose gel maker for DNA detection provided by the embodiment comprises a gel tank (6), a gel making tray rack (7) and a gel making comb (8), wherein the gel making comb (8) is arranged on the gel making tray rack (7), and the gel making tray rack (7) is arranged in the gel tank (6).
According to the horizontal agarose electrophoresis device for DNA detection and the gel maker thereof, the groove body is made of high-strength high-transparency polycarbonate material through injection molding, so that the device can be used for multiple purposes, can be used for preparing colloids with various sizes, avoids liquid leakage, is more favorable for observing an electrophoresis process in the process of gene detection or molecular detection, and enhances the operation safety due to multiple safety designs.
Drawings
FIG. 1 is a schematic structural diagram of the horizontal agarose electrophoresis apparatus for DNA detection according to an embodiment of the present invention;
FIG. 2 is a schematic structural view of the agarose gel-maker for DNA detection according to the second embodiment of the present invention;
in the figure: 1-a groove body; 2-a groove cover; 3-positive and negative electrode holders; 4-electrode groove; 5-fixing the bracket; 6-glue groove; 7-making a rubber disc rack; 8-glue making comb.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
As shown in FIG. 1, the horizontal agarose electrophoresis apparatus for DNA detection provided by the embodiment comprises a tank body 1, a tank cover 2 and a positive and negative electrode frame 3, wherein the tank body 1 is connected with the tank cover 2 in a matching way, two ends of a diagonal line of the tank body 1 are respectively provided with an electrode slot 4, and the positive and negative electrode frame 3 is respectively arranged in the electrode slot 4.
As can be understood by those skilled in the art, in the horizontal agarose electrophoresis device for DNA detection provided by the embodiment, the tank body 1 is injection molded by using a high-strength high-transparency polycarbonate material, so that the horizontal agarose electrophoresis device can be used for multiple purposes in one tank, avoids liquid leakage, is beneficial to observing an electrophoresis process, has multiple safety designs, and enhances the operation safety.
Further, the tank body 1 is of a horizontal structure.
As can be understood by those skilled in the art, filled agarose gels with various sizes can be placed on a platform of a horizontal electrophoresis tank for electrophoresis to achieve multiple purposes, and the experiment space and the experiment cost can be saved.
Further, the slot cover 2 adopts a double-ventilation slit structure.
As can be understood by those skilled in the art, the slot cover 2 adopts a double-ventilation slot design, so that fog on the slot cover 2 in the electrophoresis process is reduced, and the electrophoresis process is convenient to observe.
Further, the horizontal agarose electrophoresis device for DNA detection further comprises a fixing bracket 5, wherein the fixing bracket 5 is arranged in the middle of the groove body 1 and is clamped and connected with the inner side of the groove body 1.
Further, the tank body 1 and the cover body 2 are closed by adopting a button type cover opening structure.
Those skilled in the art will appreciate that the safety button type lid opening design facilitates the opening of the electrophoresis tank lid 2, and the multiple safety design avoids the possible operation safety problem.
Further, the electrophoresis gel is agarose gel.
As can be understood by those skilled in the art, a plurality of pieces of filled agarose gel (1-4 pieces of gel) can be stacked and placed in a gel making tray bracket for horizontal electrophoresis at the same time, so that alternative high-throughput experiments (electrophoresis experiments of up to 204 samples can be simultaneously carried out in a single groove) can be realized, and the experiment space and the experiment cost can be saved. Maximum gel-bearing area of the electrophoresis cell: 8 × 10.5cm, type of glue making tray: 8 × 10.5cm, maximum voltage load: 250V, maximum volume of holding buffer: 400ml, outer dimensions (L.times.Wtimes.H): 28X 12.5X 6.5 cm.
Example two
As shown in fig. 2, the agarose gel maker for DNA detection provided in this embodiment includes a gel tank 6, a gel making tray 7, and a gel making comb 8, wherein the gel making comb 8 is arranged on the gel making tray 7, and the gel making tray 7 is disposed in the gel tank 6.
As will be appreciated by those skilled in the art, the glue making may be accomplished in the glue tank 6 of the glue maker without the need for a tape seal. The prepared glue maker can pour 8 multiplied by 10.5cm of small glue. 3 or 2 or 1 combs can be simultaneously placed on the glue-making tray 7, so that the number of sample holes can be selected when a single glue block is prepared (9, 17, 18, 34, 27 and 51). The preparation comb of the electrophoresis tank has two tooth numbers of 9 teeth and 17 teeth, and has two optional thicknesses of 0.75mm and 1.5mm so as to meet the requirements of different sample sizes. The glue maker is injection molded by temperature resistant material (135 ℃), without deformation.
Further, the glue making comb 8 is provided with 0.75mm and 1.5mm, and the number of teeth is provided with 9 teeth and 17 teeth.
As can be understood by those skilled in the art, the glue making comb 8 (with two thicknesses of 0.75mm and 1.5mm and two tooth numbers of 9 teeth and 17 teeth) is injection-molded by temperature-resistant materials (135 ℃), is not deformed, has high dimensional precision and is not easy to damage. 1-4 pieces of gel can be stacked in the fixing bracket of the gel making disc to simultaneously carry out horizontal electrophoresis, so that optional high-throughput experiments can be realized, and electrophoresis experiments of at most 204 samples can be simultaneously carried out in a single groove.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.
Claims (7)
1. The utility model provides a horizontal agarose electrophoresis apparatus for DNA detects, its characterized in that, includes cell body (1), capping (2) and positive negative electrode frame (3), cell body (1) with capping (2) looks adaptation is connected, cell body (1) diagonal both ends are equipped with electrode slot (4) respectively, positive negative electrode frame (3) set up respectively in electrode slot (4).
2. The horizontal agarose electrophoresis device for DNA detection of claim 1, wherein the tank (1) is of a horizontal structure.
3. The horizontal agarose electrophoresis device for DNA detection of claim 2, wherein the slot cover (2) adopts a double-ventilation slit structure.
4. The horizontal agarose electrophoresis device for DNA detection of claim 3, further comprising a fixing bracket (5), wherein the fixing bracket (5) is arranged in the middle of the tank body (1) and is clamped inside the tank body (1).
5. The horizontal agarose electrophoresis device for DNA detection of claim 4, wherein the closing of the tank body (1) and the tank cover (2) adopts a button type opening structure.
6. The agarose gel maker for DNA detection is characterized by comprising a gel tank (6), a gel making tray frame (7) and a gel making comb (8), wherein the gel making comb (8) is arranged on the gel making tray frame (7), and the gel making tray frame (7) is arranged in the gel tank (6).
7. The agarose gel maker for DNA examination according to claim 6, wherein the gel making comb (8) is provided with 0.75mm and 1.5mm and the number of teeth is 9 teeth and 17 teeth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822184678.6U CN209979547U (en) | 2018-12-25 | 2018-12-25 | Horizontal agarose electrophoresis device for DNA detection and gel maker thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822184678.6U CN209979547U (en) | 2018-12-25 | 2018-12-25 | Horizontal agarose electrophoresis device for DNA detection and gel maker thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209979547U true CN209979547U (en) | 2020-01-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201822184678.6U Expired - Fee Related CN209979547U (en) | 2018-12-25 | 2018-12-25 | Horizontal agarose electrophoresis device for DNA detection and gel maker thereof |
Country Status (1)
| Country | Link |
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| CN (1) | CN209979547U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114397349A (en) * | 2022-01-14 | 2022-04-26 | 中国热带农业科学院热带生物技术研究所 | Protein electrophoresis separation method based on agarose stacking gel |
-
2018
- 2018-12-25 CN CN201822184678.6U patent/CN209979547U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114397349A (en) * | 2022-01-14 | 2022-04-26 | 中国热带农业科学院热带生物技术研究所 | Protein electrophoresis separation method based on agarose stacking gel |
| CN114397349B (en) * | 2022-01-14 | 2024-04-12 | 中国热带农业科学院热带生物技术研究所 | Protein electrophoresis separation method based on agarose stacking gel |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200121 |