CN212076908U

CN212076908U - Mammalian membrane protein extraction kit

Info

Publication number: CN212076908U
Application number: CN202020309992.7U
Authority: CN
Inventors: 蒋毅
Original assignee: Nanjing Lanlong Biotechnology Co ltd
Current assignee: Zhang Mianqun
Priority date: 2020-03-13
Filing date: 2020-03-13
Publication date: 2020-12-04
Anticipated expiration: 2030-03-13

Abstract

The utility model discloses a mammal membrane protein extraction kit, which comprises a device main body, wherein a reagent storage cavity is arranged at the right end of the device main body, a dropper storage cavity is arranged at the left end of the device main body, a top end sealing cover is arranged at the top end of the reagent storage cavity, a reagent sealing cover is arranged below the top end sealing cover, a reagent fixing frame is arranged below the reagent sealing cover, and a storage base is arranged below the reagent fixing frame; the utility model discloses effectively prevent that dust and harmful bacterium from getting into reagent storage chamber, can effectively protect the reagent pipe for reagent storage uses tighter and more clear, has improved kit storage environment, can be when reagent draws, and effective auxiliary reagent draws work, makes reagent obtain effective protection in the transport, and save set's storage security is stronger.

Description

Mammalian membrane protein extraction kit

Technical Field

The utility model belongs to the technical field of the kit, specifically a mammal membrane protein draws kit.

Background

Membrane proteins are classified into: the internal membrane protein is combined with the external membrane protein which is water-soluble protein and is combined with protein molecules or lipid molecules on the surface of the membrane by ionic bonds or other weaker bonds, so that the membrane can be separated from the membrane by changing the ionic strength of the solution and even increasing the temperature, and the membrane structure is not damaged. The essential method is to remove cytoplasmic proteins, etc. with different centrifugation speeds and then release the proteins from the membrane with detergent. The important step of membrane protein separation and purification is to select proper surfactant for solubilization, which is commonly used as cholate, ionic detergent, surfactant such as Emulgen and Lubrol, etc., the swine mycoplasma pneumonia is also called swine endemic pneumonia, commonly called swine enzootic pneumonia, which is a chronic respiratory infectious disease caused by swine mycoplasma pneumonia, the main clinical symptoms are cough and asthma, the swine mycoplasma is often mixed with porcine reproductive and respiratory syndrome and often cooperates with other multiple bacteria, viruses and environmental factors to cause the swine respiratory disease syndrome, and the asthma is often the primary cause of the swine respiratory disease syndrome. Therefore, there is an increasing demand for kits for extracting mammalian membrane proteins.

The existing membrane protein extraction kit has certain defects when in use, the storage environment of the kit is poor, the kit does not have a good sealed storage space, the reagent extraction in the kit needs tool help frequently, the kit lacks corresponding auxiliary tools, the reagent in the carrying process can not be effectively protected, the reagent is easy to spill, even a reagent bottle falls and is broken, the storage safety of a storage device is poor, and a solution is provided for solving the defects.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a mammal membrane protein draws kit mainly solves following problem:

1) by arranging the soft plug and the reagent sealing cover, when the reagent is stored, the reagent sealing cover can effectively prevent dust and harmful bacteria from entering the reagent storage cavity, the soft plug can effectively protect the reagent tubes, and further carry out sealed storage protection on the reagent, twenty groups of reagent tubes respectively correspond to the reagents, so that the reagent storage and use are tighter and clearer, the storage environment of the reagent kit is improved, two groups of burettes are arranged in the burette storage cavity, the reagent extraction work can be effectively assisted when the reagent is extracted, the fixed clamping block can effectively carry out fixed storage on the burette by matching with the tearing patch and the band, the burette sealing cover can carry out sealed protection on the burette storage cavity, so that the burette can avoid the pollution of the dust and the harmful bacteria, the problem that the existing membrane protein extraction reagent kit has poor storage environment, the reagent kit has no good sealed storage space, and the reagent extraction in, the reagent kit lacks corresponding auxiliary tools;

2) through being provided with storage base and reagent mount, the storage base can effectively protect reagent bottom safety, the reagent mount can effectively avoid the collision between individual reagent to lead to the damaged current situation of reagent pipe, the cork can effectively prevent that reagent from spilling and can effectively protect the reagent top simultaneously, make reagent obtain effective protection in the transport, save set's storage security is stronger, it can not effectively protect the reagent when the transport to have solved current membrane protein extraction kit, reagent spills easily or even the reagent bottle drops and breaks, save set's the poor technical problem of storage security.

The purpose of the utility model can be realized by the following technical scheme:

a mammalian membrane protein extraction kit comprises a device main body, wherein a reagent storage cavity is arranged at the right end of the device main body, a dropper storage cavity is arranged at the left end of the device main body, a top end sealing cover is arranged at the top end of the reagent storage cavity, a reagent sealing cover is arranged below the top end sealing cover, a reagent fixing frame is arranged below the reagent sealing cover, a storage base is arranged below the reagent fixing frame, a reagent tube is arranged at the upper end of the storage base, and a soft plug is arranged at the upper end of the reagent tube;

the novel dropper is characterized in that a dropper sealing cover is arranged at the top end of the dropper storage cavity, a fixed fixture block is arranged on the inner surface of the right end of the dropper storage cavity, a tearing patch is arranged at the left end of the fixed fixture block, a band is connected between the tearing patches, and a dropper is arranged on the right side of the band.

Preferably, the reagent tube comprises a protein extracting solution reagent tube, an ammonium acetate ethanol reagent tube, an acetone reagent tube, a lysate reagent tube, a cell washing reagent tube, a solubilization buffer reagent tube and a permeabilization buffer reagent tube.

Preferably, twenty groups of grooves are equidistantly formed in the upper end surface of the storage base, the twenty groups of grooves are divided into three groups of long strips which are transversely and equidistantly distributed on the upper end surface of the storage base, and the sizes of the twenty groups of grooves are consistent.

Preferably, twenty groups of grooves are formed in the upper end surface of the reagent fixing frame in an equidistant penetrating mode, the twenty groups of grooves are divided into three groups of long strips which are transversely distributed on the upper end surface of the reagent fixing frame at equal intervals, and the sizes of the twenty groups of grooves are consistent.

Preferably, the left end of the fixed clamping block is provided with a longitudinal groove, the longitudinal groove is cylindrical, and the width of the longitudinal groove is consistent with that of the dropper.

Preferably, the fixed fixture blocks are trapezoidal, the two groups of fixed fixture blocks are symmetrically arranged around the dropper, and the distance between the bottom end of the dropper and the bottom end of the dropper storage cavity is 1 cm.

The utility model has the advantages that:

the utility model discloses among the membrane protein draws kit, through the setting of the sealed lid of soft stopper and reagent when reagent storage, the sealed lid of reagent can effectively prevent that dust and harmful bacterium from getting into reagent storage chamber, soft stopper can effectively protect the reagent pipe, further seal the storage protection to reagent, twenty a set of reagent pipes correspond its reagent respectively, make reagent storage use tighter and more clear, kit storage environment has been improved, the burette stores the intracavity and is provided with two sets of burettes, can be when reagent draws, effective auxiliary reagent draws work, fixed fixture block cooperation is torn and is drawn the paster and the band can effectively fix the storage to the burette, the sealed lid of burette can seal the protection to burette storage chamber, make the burette avoid the pollution of dust and harmful bacterium.

The utility model discloses in the setting of storage base and reagent mount, the storage base can effectively protect reagent bottom safety, the reagent mount can effectively avoid the collision between individual reagent to lead to the damaged current situation of reagent pipe, the soft stopper can effectively prevent that reagent from spilling and can effectively protect the reagent top simultaneously, make reagent obtain effective protection in the transport, save set's storage security is stronger, it is gentle and selective detergent-based extraction method that cell cleaning reagent cooperation solubilization buffer reagent carries out animal membrane protein and draws, thereby avoided needing to carry out the problem of phase separation in the method based on the hydrophobicity, can obtain better reproducibility and higher output.

Drawings

In order to facilitate understanding for those skilled in the art, the present invention will be further described with reference to the accompanying drawings.

Fig. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic top view of the interior of the reagent storage chamber of the present invention;

FIG. 3 is a schematic diagram of a left side perspective three-dimensional structure of a dropper storage chamber of the present invention;

fig. 4 is an enlarged view of a in fig. 3 according to the present invention.

In fig. 1, a device main body; 2. a reagent storage chamber; 3. a burette storage chamber; 4. a top end sealing cover; 5. a reagent sealing cover; 6. a reagent fixing frame; 7. a storage base; 8. a reagent tube; 9. a soft plug; 10. a dropper sealing cover; 11. fixing the fixture block; 12. a dropper; 13. a protein extract reagent tube; 14. an ammonium acetate ethanol reagent tube; 15. an acetone reagent tube; 16. a lysate reagent tube; 17. a cell washing reagent tube; 18. a solubilization buffer reagent tube; 19. a permeabilization buffer reagent tube; 20. a belt; 21. the patch is torn.

Detailed Description

As shown in fig. 1-4, a mammalian membrane protein extraction kit comprises a device main body 1, wherein a reagent storage chamber 2 is arranged at the right end of the device main body 1, a dropper storage chamber 3 is arranged at the left end of the device main body 1, a top end sealing cover 4 is arranged at the top end of the reagent storage chamber 2, a reagent sealing cover 5 is arranged below the top end sealing cover 4, a reagent fixing frame 6 is arranged below the reagent sealing cover 5, a storage base 7 is arranged below the reagent fixing frame 6, a reagent tube 8 is arranged at the upper end of the storage base 7, and a soft plug 9 is arranged at the upper end of the reagent tube 8;

the improved dropper is characterized in that a dropper sealing cover 10 is arranged at the top end of the dropper storage cavity 3, a fixed clamping block 11 is arranged on the inner surface of the right end of the dropper storage cavity 3, a tearing patch 21 is arranged at the left end of the fixed clamping block 11, a band 20 is connected between the tearing patches 21, and a dropper 12 is arranged on the right side of the band 20.

The reagent tube 8 comprises a protein extracting solution reagent tube 13, an ammonium acetate ethanol reagent tube 14, an acetone reagent tube 15, a lysate reagent tube 16, a cell cleaning reagent tube 17, a solubilization buffer reagent tube 18 and a permeabilization buffer reagent tube 19, so that the reagent is stored and used more strictly and clearly, and the storage environment of the kit is improved.

Twenty groups of grooves are equidistantly formed in the upper end surface of the storage base 7, the twenty groups of grooves are divided into three groups of long strips which are transversely equidistantly distributed on the upper end surface of the storage base 7, the twenty groups of grooves are consistent in size, and the bottom of the reagent tube 8 can be effectively protected from being safe.

Reagent mount 6 upper end equidistance runs through on the surface has seted up twenty a set of recesses, and twenty a set of recesses divide into that three group's rectangular form transversely equidistance distributes on reagent mount 6 upper end surface, and twenty a set of recess sizes are unanimous, can effectively avoid the collision between individual reagent pipe 8 to lead to the damaged current situation of reagent pipe 8.

The left end of the fixed fixture block 11 is provided with a longitudinal groove, the shape of the longitudinal groove is cylindrical, the width of the longitudinal groove is consistent with that of the dropper 12, and the fixed fixture block 11 is matched with the tearing patch 21 and the binding band 20 to effectively fix and store the dropper 12.

The fixed fixture blocks 11 are trapezoidal, the two groups of fixed fixture blocks 11 are symmetrically arranged about the dropper 12, the distance between the bottom end of the dropper 12 and the bottom end of the dropper storage cavity 3 is 1cm, and the collision between the dropper 12 and the dropper storage cavity 3 can be effectively avoided.

A mammalian membrane protein extraction kit, in operation, firstly, the top end sealing cover 4 is opened, when storing a reagent, the reagent sealing cover 5 is opened, the reagent tube 8 is drawn out from the reagent fixing frame 6, if the protein extracting solution is to be stored, the protein extracting solution reagent tube 13 is drawn out, the soft plug 9 is drawn out for storing the reagent, then the reagent is placed at a corresponding position on the storage base 7 through the reagent fixing frame 6, if the ammonium acetate ethanol is to be stored, the ammonium acetate ethanol reagent tube 14 is drawn out, the soft plug 9 is drawn out for storing the reagent, then the soft plug 9 is placed at a corresponding position on the storage base 7 through the reagent fixing frame 6, the corresponding reagent tube 8 is taken away for storing in sequence according to the requirement, then the soft plug 9 is placed at a corresponding position on the storage base 7 through the reagent fixing frame 6, and the upper end of the reagent tube 8 is sealed for storing after the storing is completed, closing the reagent sealing cover 5 for sealing again, opening the dropper sealing cover 10 when extracting the reagent, opening a group of tear patches 21 and then taking the dropper 12 away, opening the reagent sealing cover 5 and the soft plug 9 on the corresponding reagent tube 8 in sequence, extracting the reagent in the dropper 12 for test, and performing animal membrane protein extraction by matching the cell cleaning reagent with the solubilization buffer reagent is a mild and selective extraction method based on the detergent, thereby avoiding the problem of phase separation in the hydrophobic method, obtaining better reproducibility and higher yield, finally placing the soft plug 9 on the corresponding reagent tube 8, closing the reagent sealing cover 5 for reagent sealing storage, cleaning the dropper 12, the left end of the fixed fixture block 11 is placed, the tearing patch 21 is matched with the binding band 20 to fix the fixed fixture block, and the dropper sealing cover 10 is closed to store in a sealing mode.

The foregoing is merely exemplary and illustrative of the structure of the invention, and various modifications, additions and substitutions as described in the detailed description may be made by those skilled in the art without departing from the structure or exceeding the scope of the invention as defined in the claims.

Claims

1. A mammalian membrane protein extraction kit comprises a device main body (1), and is characterized in that a reagent storage cavity (2) is arranged at the right end of the device main body (1), a dropper storage cavity (3) is arranged at the left end of the device main body (1), a top end sealing cover (4) is arranged at the top end of the reagent storage cavity (2), a reagent sealing cover (5) is arranged below the top end sealing cover (4), a reagent fixing frame (6) is arranged below the reagent sealing cover (5), a storage base (7) is arranged below the reagent fixing frame (6), a reagent tube (8) is arranged at the upper end of the storage base (7), and a soft plug (9) is arranged at the upper end of the reagent tube (8);

the novel dropper is characterized in that a dropper sealing cover (10) is arranged at the top end of the dropper storage cavity (3), a fixed clamping block (11) is arranged on the inner surface of the right end of the dropper storage cavity (3), a tearing patch (21) is arranged at the left end of the fixed clamping block (11), a band (20) is connected between the tearing patches (21), and a dropper (12) is arranged on the right side of the band (20).

2. The mammalian membrane protein extraction kit according to claim 1, wherein the reagent tube (8) comprises a protein extract reagent tube (13), an ammonium acetate ethanol reagent tube (14), an acetone reagent tube (15), a lysate reagent tube (16), a cell washing reagent tube (17), a solubilization buffer reagent tube (18), and a permeabilization buffer reagent tube (19).

3. A mammalian membrane protein extraction kit according to claim 1, wherein twenty sets of grooves are formed on the upper end surface of the storage base (7) at equal intervals, and the twenty sets of grooves are divided into three groups of long strips which are transversely and equidistantly distributed on the upper end surface of the storage base (7), and the sizes of the twenty sets of grooves are the same.

4. The mammalian membrane protein extraction kit according to claim 1, wherein twenty sets of grooves are formed on the upper end surface of the reagent fixing frame (6) at equal intervals, and the twenty sets of grooves are divided into three groups of long strips which are transversely and equidistantly distributed on the upper end surface of the reagent fixing frame (6), and the sizes of the twenty sets of grooves are the same.

5. The mammalian membrane protein extraction kit according to claim 1, wherein the left end of the fixed fixture block (11) is provided with a longitudinal groove, the longitudinal groove is cylindrical, and the width of the longitudinal groove is consistent with the width of the dropper (12).

6. The mammalian membrane protein extraction kit according to claim 1, wherein the fixed fixture blocks (11) are trapezoidal, and two groups of fixed fixture blocks (11) are symmetrically arranged about the dropper (12), and the distance between the bottom end of the dropper (12) and the bottom end of the dropper storage chamber (3) is 1 cm.