CN215525630U

CN215525630U - Gel dyeing rinsing box with bottom leakage

Info

Publication number: CN215525630U
Application number: CN202121130386.XU
Authority: CN
Inventors: 宣佳雯; 陈功星; 谢恬; 刘茂林; 王家俊; 刘安敏; 陆思敏; 马又瑜; 顾胜喜; 赵起超
Original assignee: Hangzhou Normal University
Current assignee: Hangzhou Normal University
Priority date: 2021-05-25
Filing date: 2021-05-25
Publication date: 2022-01-14
Anticipated expiration: 2031-05-25

Abstract

The utility model discloses a gel dyeing rinsing box with a bottom leakage opening. The utility model comprises a rinsing box body, a sealing plug and a connecting rope. And a leakage opening is formed in one corner of the bottom of the rinsing box body. The bottom of the leakage opening is provided with a drainage nozzle. The drainage nozzle is wide at the top and narrow at the bottom. The sealing plug is columnar, and the shape of the cross section corresponds to the leakage opening and the drainage nozzle. The rinsing box is simple in structure, and the dyeing liquid and the rinsing liquid can be poured out from the bottom of the rinsing box, so that the risk that gel is separated from the bottom of the box and falls off can be avoided. The rinsing box is simple in structure, and the dyeing liquid and the rinsing liquid can be poured out from the bottom of the rinsing box, so that the risk that gel is separated from the bottom of the box and falls off can be avoided. In addition, the utility model can lock the sealing plug on the leakage opening by matching the telescopic elastic column with the locking hole on the rinsing box body, thereby avoiding the leakage of the rinsing box in the rinsing process.

Description

Gel dyeing rinsing box with bottom leakage

Technical Field

The utility model belongs to the technical field of gel dyeing equipment, and particularly relates to a gel dyeing rinsing box with a bottom leakage opening.

Background

Gel electrophoresis is widely used in the fields of molecular biology, genetics, biochemistry and the like, and is commonly used in the analysis of proteins and nucleic acids (including single cell gel electrophoresis), including agarose and polyacrylamide gel electrophoresis, pulsed electric field gel electrophoresis and the like.

In proteomics experiments, protein staining after polyacrylamide gel electrophoresis is an important link. The method for forming a colored complex by combining dye and biological macromolecules is a common method for detection after electrophoresis, and the current dyeing methods comprise iodine dyeing, Coomassie brilliant blue dyeing, silver dyeing, CuCl dyeing and the like. In the experiment of efficiently separating and analyzing nucleic acid molecules by agarose gel electrophoresis, the detection of DNA after electrophoresis is a key link and is directly related to whether the experimental result is displayed or not. The commonly used DNA detection method on agarose gel includes fluorescence staining method, dye staining method, metal ion staining method, negative staining method, etc. Most dyeing methods require rinsing and bleaching after dyeing for observation.

In the process of dyeing and rinsing gel, dyeing liquid and rinsing liquid need to be poured, the dyeing and rinsing of the existing appliance box are directly poured from the opening of the box, and the gel can exceed 90 degrees and risk of falling off the bottom of the box. If reagent recovery is involved, the liquid may spill out during pouring and be wasted, or even cause reagent contamination.

Disclosure of Invention

The utility model aims to provide a gel dyeing and rinsing box with a bottom leakage opening.

The utility model comprises a rinsing box body, a sealing plug and a connecting rope. And a leakage opening is formed in one corner of the bottom of the rinsing box body. The bottom of the leakage opening is provided with a drainage nozzle. The drainage nozzle is wide at the top and narrow at the bottom. The sealing plug is columnar, and the shape of the cross section corresponds to the leakage opening and the drainage nozzle.

Preferably, the rinsing box body is provided with a U-shaped baffle at a position corresponding to the leakage opening. The U-shaped baffle comprises a transverse plate and two side plates which are integrally formed. The same side edges of the two side plates are respectively connected with the two sides of the transverse plate. The other side edges of the two side plates are fixed with the inner side wall of the rinsing box body. The two side plates of the U-shaped baffle are provided with locking holes.

The sealing plug comprises a main plug body, a locking spring and two locking rods. First guide holes are formed in two sides of the inner end of the side face of the plug body. The second guide hole has all been seted up to the both sides of the outer end of main cock body side. A cavity is arranged in the main plug body. The main lock rod comprises a locking column, a connecting rod and a pressing column. The locking column, the pressing column and the two ends of the connecting rod are respectively fixed. The locking column and the pressing column on one lock rod are respectively connected with the first guide hole and the second guide hole on one side of the main plug body in a sliding mode. The locking column and the pressing column on the other lock rod are respectively connected with the first guide hole and the second guide hole on the other side of the main plug body in a sliding manner. A plurality of springs are arranged between the two lock rods.

Preferably, the main plug body is wrapped with an elastic layer.

Preferably, the bottom edge of the U-shaped baffle is arranged at a distance from the bottom surface of the inner cavity of the rinsing box body.

Preferably, the inner end of the sealing plug is chamfered.

Preferably, the rinsing box body is a box body with a large top and a small bottom, and the top of the rinsing box body is opened. The top edge of rinsing box body is provided with the evagination along.

Preferably, each corner of the rinsing box body is arc-shaped.

Preferably, the side wall of the rinsing box body is provided with scale marks.

Preferably, a box cover is arranged at the top of the rinsing box body.

Preferably, the top surface of the rinsing box body is a rectangle with the length of 11-28 cm and the width of 9-24 cm, and the bottom surface of the rinsing box body is a rectangle with the length of 9-26 cm and the width of 7-22 cm; the height of the rinsing box body 1 is 5cm, and the wall thickness is 0.5-1 cm.

The utility model has the beneficial effects that:

1. the rinsing box is simple in structure, and the dyeing liquid and the rinsing liquid can be poured out from the bottom of the rinsing box, so that the risk that gel is separated from the bottom of the box and falls off can be avoided.

2. According to the utility model, the flexible elastic column is matched with the locking hole on the rinsing box body, so that the sealing plug can be locked on the leakage opening, and the rinsing box is prevented from leaking in the rinsing process.

3. The U-shaped baffle plate can prevent the gel from leaking out of the leakage opening, so that the gel is prevented from being damaged.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic top view of the present invention;

FIG. 3 is an enlarged view of a portion A of FIG. 2;

FIG. 4 is a schematic front view of the present invention with the plug removed;

FIG. 5 is a partial enlarged view of portion B of FIG. 4;

fig. 6 is a perspective view of the sealing plug of the present invention;

fig. 7 is a schematic cross-sectional view of a sealing plug according to the present invention.

Detailed Description

The utility model is further described below with reference to the accompanying drawings.

As shown in fig. 1 and 2, a gel dyeing rinsing box with a bottom leakage opening comprises a rinsing box body 1, a sealing plug 2, a connecting rope 3 and a U-shaped baffle 4. The rinsing box body 1 is in a box body shape with a large top and a small bottom, and the top is arranged in an open mode. The top edge of the rinsing box body 1 is provided with an outer convex edge 3-1. The outer convex edge 3-1 is used for arranging the gel on a shaking table for dyeing and rinsing when a plurality of groups of experiments are carried out, the design of the trapezoid body with the large upper part and the small lower part meets the requirement of stacking gel dyeing and rinsing boxes, and the operation space required by the experiments is saved. Each corner of the rinsing box body 1 is arc-shaped. The side wall of the rinsing box body 1 is provided with scale marks for quantitatively dyeing and rinsing. The top of the rinsing box body 1 is provided with a matched box cover, so that the rinsing box can be protected from light and can reduce pollution to a certain extent. The top surface of the rinsing box body 1 is a rectangle with the length of 11-28 cm and the width of 9-24 cm, and the bottom surface of the rinsing box body is a rectangle with the length of 9-26 cm and the width of 7-22 cm; the height of the rinsing box body 1 is 5cm, and the wall thickness is 0.5-1 cm. Different sizes of dye rinse cassettes can be used depending on gel size.

As shown in fig. 4 and 5, a leakage opening 1-2 is arranged on one corner of the bottom of the rinsing box body 1. The leakage opening 1-2 is of a quasi-circular shape with the diameter of 1-2 cm. The bottom of the leakage opening 1-2 is provided with a olecranon-shaped drainage nozzle. The drainage nozzle is wide at the top and narrow at the bottom, so that water flow can be collected conveniently, the flow speed of liquid can be controlled conveniently, and meanwhile, a dyeing reagent can be recovered through the drainage nozzle. The distance between the leakage opening 1-2 and the bottom of the rinsing box body 1 is 0.5-1 cm.

The U-shaped baffle 4 is arranged in the inner cavity of the rinsing box body 1 corresponding to the position of the leakage opening 1-2. The U-shaped baffle 4 comprises a transverse plate and two side plates which are integrally formed. The same side edges of the two side plates are respectively connected with the two sides of the transverse plate. The other side edges of the two side plates are fixed with the inner side wall of the rinsing box body 1. The bottom edge of the U-shaped baffle 4 is arranged at intervals with the bottom surface of the inner cavity of the rinsing box body 1 so as to facilitate the liquid in the rinsing box body 1 to flow out from the leakage opening 1-2. The two side plates of the U-shaped baffle 4 are provided with locking holes. The U-shaped baffle 4 has two functions, on one hand, the gel in the rinsing box body 1 can be prevented from flowing out from the leakage openings 1-2, and on the other hand, the sealing plug 2 and the rinsing box body 1 can be fixed by utilizing the position locking hole.

As shown in fig. 3, 6 and 7, the sealing plug 2 is cylindrical and has a cross-sectional shape corresponding to the discharge opening 1-2 and the drainage nozzle, for sealing the discharge opening 1-2 and the drainage nozzle during rinsing or dyeing to prevent the reagents from leaking out. When the sealing plug 2 is plugged into the leakage opening 1-2, the leakage opening 1-2 can be completely sealed. The sealing plug 2 comprises a main plug body 2-1, an elastic layer 2-2, a locking spring 2-3 and two locking rods. The elastic layer 2-2 is wrapped on the outer side of the main plug body 2-1 to ensure the sealing performance and the tightness of the sealing plug 2. The inner end part of the elastic layer 2-2 is provided with a chamfer angle, so that the sealing plug 2 can be conveniently plugged into the leakage opening 1-2. The main plug body 2-1 is internally provided with a cavity used for installing a locking spring 2-3 and two locking rods. The two sides of the inner end of the side surface of the main plug body 2-1 are both provided with first guide holes. And second guide holes are formed in two sides of the outer end of the side face of the main plug body 2-1. The main plug body 2-1 and the lock rod are made of plastics. The capture springs 2-3 are SPEC plastic springs. The elastic layer 2-2 is made of rubber. So that the entire sealing plug 2 does not involve metal, avoiding the sealing plug 2 reacting with acidic substances in the liquid of the rinsing glue.

The lock rod comprises a locking column 2-4, a connecting rod 2-5 and a pressing column 2-6. The locking column 2-4, the pressing column 2-6 and the two ends of the connecting rod 2-5 are respectively fixed. The axes of the locking column 2-4 and the pressing column 2-6 are parallel and are perpendicular to the axis of the connecting rod 2-5. The locking column 2-4 and the pressing column 2-6 on one lock rod are respectively connected with the first guide hole and the second guide hole on one side of the main plug body 2-1 in a sliding manner. The locking column 2-4 and the pressing column 2-6 on the other lock rod are respectively connected with the first guide hole and the second guide hole on the other side of the main plug body 2-1 in a sliding manner. A plurality of springs are arranged between the two lock rods, so that the locking columns 2-4 and the pressing columns 2-6 on the two lock rods can be outwards ejected. When the sealing plug 2 is plugged into the leakage opening 1-2, the two locking columns 2-4 are respectively clamped into the two locking holes on the U-shaped baffle 4, so that the sealing plug 2 is locked. When the sealing plug 2 needs to be taken down, the two pressing columns 2-6 are squeezed by two fingers to separate the locking columns 2-4 from the locking hole, so that the sealing plug 2 can be taken out by pulling outwards.

One end of the connecting rope 3 is fixed with the middle part of the outer end of the sealing plug 2 through a slipknot, and the other end of the connecting rope is bonded with the edge of the outer side wall of the rinsing box body 1 or connected with the edge of the outer side wall of the rinsing box body through the slipknot. The connecting rope 3 avoids being lost after the sealing plug 2 is pulled out. The connecting rope 3 is a nylon rope.

Example 2

This example differs from example 1 in that: the outer end face of the movable plug is provided with a cross-shaped bracket for fixedly connecting the rope 3.

Example 3

This example differs from example 1 in that: the main plug body 2-1 in the sealing plug 2 adopts a frame type structure so as to provide a force application point for the connecting rope 3.

Claims

1. A gel dyeing rinsing box with a bottom leakage opening comprises a rinsing box body (1) and a sealing plug (2); the method is characterized in that: a leakage opening (1-2) is formed in one corner of the bottom of the rinsing box body (1); the bottom of the leakage opening (1-2) is provided with a drainage nozzle; the cross section of the sealing plug (2) corresponds to the leakage opening (1-2) and the drainage nozzle.

2. A gel stain rinse cassette with bottom drain as claimed in claim 1, wherein: a U-shaped baffle (4) is arranged in the inner cavity of the rinsing box body (1) corresponding to the position of the leakage opening (1-2); the U-shaped baffle (4) comprises a transverse plate and two side plates which are integrally formed; the same side edges of the two side plates are respectively connected with the two sides of the transverse plate; the other side edges of the two side plates are fixed with the inner side wall of the rinsing box body (1); two side plates of the U-shaped baffle (4) are both provided with a locking hole; the sealing plug (2) comprises a main plug body (2-1), a locking spring (2-3) and two locking rods; two sides of the inner end of the side surface of the plug body (2-1) are provided with first guide holes; two sides of the outer end of the side surface of the main plug body (2-1) are provided with second guide holes; a cavity is arranged in the main plug body (2-1); the main locking rod comprises a locking column (2-4), a connecting rod (2-5) and a pressing column (2-6); the locking column (2-4) and the pressing column (2-6) are respectively fixed with the two ends of the connecting rod (2-5); a locking column (2-4) and a pressing column (2-6) on one lock rod are respectively connected with a first guide hole and a second guide hole on one side of the main plug body (2-1) in a sliding manner; a locking column (2-4) and a pressing column (2-6) on the other lock rod are respectively connected with a first guide hole and a second guide hole on the other side of the main plug body (2-1) in a sliding manner; a plurality of springs are arranged between the two lock rods.

3. A gel staining rinsing cartridge with bottom drain as claimed in claim 2, characterized in that: the outer side of the main plug body (2-1) is wrapped with an elastic layer (2-2).

4. A gel staining rinsing cartridge with bottom drain as claimed in claim 2, characterized in that: the bottom edge of the U-shaped baffle (4) is arranged at intervals with the bottom surface of the inner cavity of the rinsing box body (1).

5. A gel-staining rinsing cartridge with a bottom drain according to claim 1 or 2, characterized in that: a chamfer is arranged at the end part of the inner end of the sealing plug (2); the drainage mouth is wide at the top and narrow at the bottom.

6. A gel-staining rinsing cartridge with a bottom drain according to claim 1 or 2, characterized in that: the rinsing box body (1) is in a box shape with a large top and a small bottom, and the top of the rinsing box body is opened; an outer convex edge (3-1) is arranged at the top edge of the rinsing box body (1); each corner of the rinsing box body (1) is arc-shaped.

7. A gel-staining rinsing cartridge with a bottom drain according to claim 1 or 2, characterized in that: the outer side surface of the rinsing box body (1) is connected with the sealing plug (2) through a connecting rope (3).

8. A gel-staining rinsing cartridge with a bottom drain according to claim 1 or 2, characterized in that: the side wall of the rinsing box body (1) is provided with scale marks.

9. A gel-staining rinsing cartridge with a bottom drain according to claim 1 or 2, characterized in that: the top of the rinsing box body (1) is provided with a box cover.

10. A gel-staining rinsing cartridge with a bottom drain according to claim 1 or 2, characterized in that: the top surface of the rinsing box body (1) is a rectangle with the length of 11-28 cm and the width of 9-24 cm, and the bottom surface of the rinsing box body is a rectangle with the length of 9-26 cm and the width of 7-22 cm; the height of the rinsing box body 1 is 5cm, and the wall thickness is 0.5-1 cm.