CN212982915U - Nucleic acid extraction kit - Google Patents
Nucleic acid extraction kit Download PDFInfo
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- CN212982915U CN212982915U CN202021078251.9U CN202021078251U CN212982915U CN 212982915 U CN212982915 U CN 212982915U CN 202021078251 U CN202021078251 U CN 202021078251U CN 212982915 U CN212982915 U CN 212982915U
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Abstract
The utility model provides a nucleic acid extraction kit, include the backup pad and install the nucleic acid extraction kit body in the backup pad, the box body in be equipped with the schizolysis cabin body washing cabin body and the elution cabin body of arranging side by side, the box body in still be equipped with heating module, heating module including arranging the first heating module in schizolysis cabin body bottom and arranging the second heating module in elution cabin body bottom, first heating module can wrap up the bottom in the schizolysis cabin body in addition, the bottom in the elution cabin body can be wrapped up to the second heating module. The fixing mode of the utility model is that the elastic bodies at the two sides of the kit are deformed and fastened, the fixing device does not need to be deformed, and the loss of the fixing device is avoided; the reagent box cracking cabin is larger than other cabins, the sample size is large, the wrapping area of the heating mechanism at the bottom of the cracking cabin is large, the heating speed is high, and the heat preservation effect is good. Overall structure is simple, the simple operation.
Description
Technical Field
The utility model belongs to the technical field of nucleic acid extraction, concretely relates to nucleic acid extraction kit.
Background
With the rapid development of molecular biology techniques in recent years, molecular diagnosis and detection techniques typified by nucleic acid hybridization, nucleic acid amplification, and nucleic acid sequence analysis have increasingly highlighted important roles in various fields. Novel molecular biological detection techniques include Polymerase Chain Reaction (PCR), microchips, high-throughput sequencing, and the like. However, all modern molecular biology detection techniques must rapidly and effectively isolate and extract the desired genomic nucleic acid from complex and diverse biological samples, and the quality and integrity of the extracted nucleic acid directly affect the results of subsequent experiments.
The magnetic rod method is a nucleic acid purification method which is developed rapidly in recent years, and the magnetic bead separation technology is adopted, and the collection, release, transfer and incubation of magnetic beads are realized through the movement of a magnetic rod and a magnetic sleeve, so that the whole extraction process of nucleic acid is completed.
At present, the fixing mode of a nucleic acid extraction kit matched with nucleic acid extraction equipment of a plurality of manufacturers is that the fixing device is fastened by elastic deformation, and the fixing device is easy to wear and needs to be replaced frequently; the sizes of all cabin bodies of the kit are the same, the wrapping area of a heating mechanism at the bottom of the cracking cabin is small, the heating speed is low, and the heat preservation effect is general.
Disclosure of Invention
To above-mentioned prior art not enough with the defect, the utility model aims to provide a nucleic acid extraction kit, solve among the prior art easy loss of fixing device of fixed mode, need frequently to change and each cabin body of kit is the same in size, schizolysis cabin bottom heating mechanism parcel area is less, and rate of heating is slow and the general technical problem of heat preservation effect.
In order to achieve the purpose, the technical scheme is as follows:
a nucleic acid extraction kit comprises a support plate and a nucleic acid extraction kit body arranged on the support plate, wherein the nucleic acid extraction kit body comprises a kit body connected with the support plate and an upper cover connected on the kit body;
the box body in be equipped with the schizolysis cabin body, the washing cabin body and the elution cabin body of arranging side by side, the box body in still be equipped with heating module, heating module including arranging the first heating module in schizolysis cabin body bottom and arranging the second heating module in elution cabin body bottom, first heating module can wrap up the bottom in the schizolysis cabin body in addition, the bottom in the elution cabin body can be wrapped up to the second heating module, first heating module and second heating module can heat respectively.
The utility model discloses still have following technical characteristic:
the box body is connected with the supporting plate through fixing devices, and the fixing devices exist in pairs and are connected to two sides of the box body.
The number of the cracking cabin bodies is multiple, and the cracking cabin bodies comprise a plurality of cracking cabin bodies which are arranged side by side;
the washing cabin bodies are multiple, and each washing cabin body comprises a plurality of washing cabins which are arranged side by side;
the elution cabin body be a plurality of, the elution cabin body in include a plurality of elution cabins of arranging side by side.
The box body including first box body and the second box body of arranging side by side, first box body and second box body in the equipartition put a plurality of schizolysis cabin bodies and elution cabin body, first box body and second box body pass through the compartment and connect.
The fixing device comprises a fixing block and an elastic body connected to the fixing block, and the elastic body is used for fixing the nucleic acid extraction kit body.
Compared with the prior art, the utility model, profitable technological effect is:
the fixing mode of the utility model is that the elastic bodies on the two sides of the kit are deformed and fastened, the fixing device does not need to be deformed, and the loss of the fixing device is avoided;
(II) the utility model discloses the kit schizolysis cabin is big than other cabins, and the sample volume is big, and schizolysis cabin bottom heating mechanism parcel area is more, and rate of heating is very fast, and the heat preservation effect is better.
(III) the utility model has the advantages of simple integral structure, convenient operation and strong practicability.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic cross-sectional view of the present invention;
the meaning of the individual reference symbols in the figures is: 1-supporting plate, 2-nucleic acid extraction kit body, 3-box body, 4-upper cover, 5-cracking cabin body, 6-washing cabin body, 7-elution cabin body, 8-heating module, 8-1-first heating module, 8-2-second heating module, 9-fixing device, 10-cracking cabin body, 11-washing cabin body, 12-elution cabin body, 13-first box body, 14-second box body, 15-compartment, 16-fixing block and 17-elastomer.
The following detailed description of the present invention will be made with reference to the accompanying drawings and examples.
Detailed Description
The following embodiments of the present invention are given, and it should be noted that the present invention is not limited to the following embodiments, and all the equivalent transformations made on the basis of the technical solution of the present application all fall into the protection scope of the present invention.
Example 1:
following the above technical solution, as shown in fig. 1 and fig. 2, this embodiment provides a nucleic acid extraction kit, which includes a support plate 1 and a nucleic acid extraction kit body 2 installed on the support plate 1, wherein the nucleic acid extraction kit body 2 includes a box body 3 connected to the support plate 1 and an upper cover 4 connected to the box body 3;
the box body 3 is internally provided with a cracking cabin body 5, a washing cabin body 6 and an elution cabin body 7 which are arranged side by side, the box body 3 is internally further provided with a heating module 8, the heating module 8 comprises a first heating module 8-1 arranged at the bottom of the cracking cabin body 5 and a second heating module 8-2 arranged at the bottom of the elution cabin body 7, the first heating module 8-1 can wrap the bottom of the cracking cabin body 5, and the second heating module 8-2 can wrap the bottom of the elution cabin body 7. The heating module 8 is used for heating the cracking cabin body 5 and the elution cabin body 7, the first heating module 8-1 and the second heating module 8-2 can respectively heat, the bottom of the first heating module is in a thin concave shape, the heating modules are wrapped more than a conventional kit, the heating effect is better, the cracking cabin body 5 and the elution cabin body 7 are larger than the conventional kit, the sample containing capacity is large, and target nucleic acid is easier to obtain.
The lysis chamber 5 contains lysis solution for cell lysis in the sample and sufficient combination of nucleic acid and magnetic beads.
The washing chamber 6 contains a washing solution for washing away impurities other than the combination of the target nucleic acid and the magnetic beads after the completion of the lysis.
The elution cabin 7 contains an eluent for completely separating the target nucleic acid from the magnetic beads after washing is finished, and the target nucleic acid is obtained after the magnetic beads are removed.
The box body 3 is connected with the supporting plate 1 through a fixing device 9, and the fixing devices 9 exist in pairs and are connected to two sides of the box body 3. The fixing device 9 is used for fixing the nucleic acid extraction kit body 2.
As a preferred one of this embodiment, the cracking cabin 5 is plural, the cracking cabin 5 includes plural cracking chambers 10, the washing cabin 6 is plural, the washing cabin 6 includes plural washing chambers 11, the elution cabin 7 includes plural elution chambers 12, and the elution cabin 7 includes plural elution chambers 12. The arrangement of a plurality of cabin bodies makes the utility model discloses there is bigger range of application. The upper surface of the box body 3 is also provided with position marks, so that the position of each cabin body can be conveniently confirmed.
As a preference of this embodiment, the cartridge 3 includes a first cartridge 13 and a second cartridge 14 arranged side by side, a plurality of lysis chambers 5 and elution chambers 7 are disposed in the first cartridge 13 and the second cartridge 14, and the first cartridge 13 and the second cartridge 14 are connected by a compartment 15. The compartment 15 is used for separating the first box body 13 from the second box body 14, and cross infection caused by aerosol is prevented.
As shown in fig. 2, in the present embodiment, a cracking chamber 5, three washing chambers 6, an elution chamber 7, a separation chamber 15, a cracking chamber 5, three washing chambers 6, an elution chamber 7, and a washing chamber 6 are respectively arranged from left to right. The left cracking cabin 5, the three washing cabins 6 and the elution cabin 7 form a first box body 13, and the right cracking cabin 5, the three washing cabins 6, the elution cabin 7 and the washing cabin 6 form a second box body 14.
As shown in fig. 1, each lysis chamber 5 comprises 8 lysis chambers 10, each wash chamber 6 comprises 8 wash chambers 11, and each elution chamber 7 comprises 8 elution chambers 12.
The specific number of settings is determined according to actual requirements.
As a preference of this embodiment, the fixing device 9 comprises a fixing block 16 and an elastic body 17 connected to the fixing block 16, and the elastic body 17 is used for fixing the nucleic acid extraction kit body 2. Make the utility model discloses nucleic acid extraction kit body 2 is more stable when using, extracts target nucleic acid more easily.
The nucleic acid extraction kit is a disposable consumable product, and the kit is fixed by using the elastomer, so that the loss of a fixing device is avoided, frequent replacement is not needed, and the maintenance frequency of equipment is reduced.
The utility model discloses the use step as follows:
magnetic beads with nucleic acid adsorbed are moved to different reagent holes by using a magnetic rod on a magnetic rod rack in the machine, then liquid is rapidly stirred repeatedly by using a stirring sleeve sleeved on the outer layer of the magnetic rod, so that the liquid and the magnetic beads are uniformly mixed, and the high-purity nucleic acid is finally obtained through cell lysis, nucleic acid adsorption, washing and elution.
The first step is as follows: taking down the upper cover 4, and manually adding the sample into the cracking cabin body 5 according to the test requirement;
the second step is that: correctly installing the box body 3 added with the sample on the supporting plate 1, and enabling the elastic bodies 17 at two sides of the box body 3 to be matched with the fixing block 16 to be fastened;
the third step: adding lysis solution into the lysis cabin body 5, heating the lysis cabin body 5 to a lysis temperature by the first heating module 8-1, then repeatedly stirring the lysis solution by the stirring sleeve to uniformly mix the lysis solution with magnetic beads, and fully combining nucleic acid and the magnetic beads after cell lysis;
the fourth step: adding a washing solution into the washing cabin body 6, inserting a stirring sleeve to enable magnetic beads to be adsorbed on the stirring sleeve inserted with the magnetic rod, transferring the magnetic rod into the washing cabin body 6, moving the magnetic rod out of the stirring sleeve, then repeatedly stirring the washing solution by using the stirring sleeve to enable the washing solution and the magnetic beads adsorbed with nucleic acid to be uniformly mixed, and washing away impurities; the arrangement of a plurality of washing chambers 6 makes it possible to repeat the fourth step several times and wash out impurities more thoroughly.
The fifth step: eluent is added into the elution cabin body 7, the second heating module 8-2 heats the elution cabin body 7 to an elution temperature, the magnetic rod is inserted into the stirring sleeve, magnetic beads are adsorbed on the stirring sleeve inserted with the magnetic rod, the magnetic rod is transferred into the elution cabin body 7, the magnetic rod is moved out of the stirring sleeve, then the eluent is repeatedly stirred by the stirring sleeve, the eluent is uniformly mixed with the magnetic beads adsorbing nucleic acids, after the target nucleic acids are completely separated from the magnetic beads, the magnetic rod is inserted into the stirring sleeve, the magnetic beads are moved out, the target nucleic acids are obtained, and an experiment is completed.
Claims (5)
1. A nucleic acid extraction kit comprises a support plate (1) and a nucleic acid extraction kit body (2) arranged on the support plate (1), and is characterized in that the nucleic acid extraction kit body (2) comprises a kit body (3) connected with the support plate (1) and an upper cover (4) connected with the kit body (3);
the box body (3) is internally provided with a cracking cabin body (5), a washing cabin body (6) and an elution cabin body (7) which are arranged side by side, the box body (3) is internally provided with a heating module (8), and the heating module (8) comprises a first heating module (8-1) arranged at the bottom of the cracking cabin body (5) and a second heating module (8-2) arranged at the bottom of the elution cabin body (7).
2. The nucleic acid extraction kit according to claim 1, wherein the cartridge body (3) is connected to the support plate (1) by a fixing means (9), and the fixing means (9) are present in pairs and connected to both sides of the cartridge body (3).
3. The nucleic acid extraction kit according to claim 1, wherein the lysis chamber (5) is a plurality of lysis chambers, and the lysis chamber (5) comprises a plurality of lysis chambers (10) arranged side by side;
the number of the washing cabin bodies (6) is multiple, and the washing cabin bodies (6) comprise a plurality of washing cabins (11) which are arranged side by side;
the elution cabin body (7) is multiple, and the elution cabin body (7) comprises a plurality of elution cabins (12) which are arranged side by side.
4. The nucleic acid extraction kit according to claim 1, wherein the cartridge (3) comprises a first cartridge (13) and a second cartridge (14) arranged side by side, the first cartridge (13) and the second cartridge (14) each having a lysis compartment (5), a washing compartment (6) and an elution compartment (7) disposed therein, the first cartridge (13) and the second cartridge (14) being connected by a compartment (15).
5. The nucleic acid extraction kit according to claim 2, wherein the fixing means (9) comprises a fixing block (16) and an elastic body (17) connected to the fixing block (16), and the elastic body (17) is used for fixing the nucleic acid extraction kit body (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021078251.9U CN212982915U (en) | 2020-06-11 | 2020-06-11 | Nucleic acid extraction kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021078251.9U CN212982915U (en) | 2020-06-11 | 2020-06-11 | Nucleic acid extraction kit |
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| Publication Number | Publication Date |
|---|---|
| CN212982915U true CN212982915U (en) | 2021-04-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021078251.9U Active CN212982915U (en) | 2020-06-11 | 2020-06-11 | Nucleic acid extraction kit |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114410429A (en) * | 2022-02-08 | 2022-04-29 | 深圳潘道医学检验实验室 | High-purification-degree automatic nucleic acid extraction instrument |
-
2020
- 2020-06-11 CN CN202021078251.9U patent/CN212982915U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114410429A (en) * | 2022-02-08 | 2022-04-29 | 深圳潘道医学检验实验室 | High-purification-degree automatic nucleic acid extraction instrument |
| CN114410429B (en) * | 2022-02-08 | 2024-03-15 | 深圳潘道医学检验实验室 | High-purification-degree automatic nucleic acid extraction instrument |
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