CN212925026U - Sampling tube capable of quantitatively transferring samples - Google Patents
Sampling tube capable of quantitatively transferring samples Download PDFInfo
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- CN212925026U CN212925026U CN202021600732.1U CN202021600732U CN212925026U CN 212925026 U CN212925026 U CN 212925026U CN 202021600732 U CN202021600732 U CN 202021600732U CN 212925026 U CN212925026 U CN 212925026U
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Abstract
The utility model provides a sampling pipe that can quantitative transfer sample, the sampling pipe includes from last to at least down: the tube cover comprises a cover bottom and a cover cap, the cover bottom is covered with the sample collecting hose, and the cover cap is arranged above the cover bottom and forms a sample transferring cavity with the cover bottom; the top of the cap is provided with a first liquid outlet hole communicated with the outside; the aperture of the first liquid outlet is 1mm-5 mm; a second liquid outlet hole for communicating the sample collection hose with the sample transfer cavity is formed in the cover bottom; the tube cap further comprises a top cap, and the top cap can be detachably covered on the cap. The utility model discloses a sampling pipe that can quantitative transfer sample, very big degree has reduced the safety risk that probably brings with professional liquid-transfering gun equipment ration transfer sample after the fulminating infectious disease sample is uncapped, and this kind of application of sample mode does not need equipment such as professional liquid-transfering gun, also can standardized quantitative application of sample, uses extremely simplely moreover.
Description
Technical Field
The utility model relates to a biotechnology apparatus field especially relates to a sampling pipe that can quantitative transfer sample.
Background
The gene screening or diagnosis has been widely used due to its characteristics of strong detection specificity, high sensitivity, short window period, etc., and has been widely applied to various scenes such as clinic, disease prevention and control, food safety detection, import and export quarantine detection, etc., and the rapid development of 20% is presented every year. The types of the related samples are more abundant and diversified, particularly in the front line of disease prevention and control and import and export inspection and quarantine, the requirements of the sample pretreatment before inspection on the field inactivation of the related pathogens and the room temperature transportation of the inactivated liquid samples are extremely high, and particularly for the virulent infectious diseases, the sample tube does not suggest to open the cover to transfer the samples due to the biological safety, which provides a serious challenge for the existing detection sample pretreatment technology.
SUMMERY OF THE UTILITY MODEL
In view of the above-mentioned shortcomings of the prior art, it is an object of the present invention to provide a sampling tube capable of quantitatively transferring a sample.
In order to achieve the above objects and other related objects, the utility model provides a sampling pipe that can transfer sample quantitatively, the sampling pipe includes from last to at least down: the tube cover comprises a cover bottom and a cover cap, the cover bottom is covered with the sample collecting hose, and the cover cap is arranged above the cover bottom and forms a sample transferring cavity with the cover bottom; the top of the cap is provided with a first liquid outlet hole communicated with the outside; the aperture of the first liquid outlet is 1mm-5 mm; a second liquid outlet hole for communicating the sample collection hose with the sample transfer cavity is formed in the cover bottom; the tube cap further comprises a top cap, and the top cap can be detachably covered on the cap.
As above, the utility model discloses a sampling pipe that can transfer sample by ration has following beneficial effect:
the utility model discloses a sampling pipe that can quantitative transfer sample, very big degree has reduced the safety risk that probably brings with professional liquid-transfering gun equipment ration transfer sample after the fulminating infectious disease sample is uncapped, and this kind of application of sample mode does not need equipment such as professional liquid-transfering gun, also can standardized quantitative application of sample, uses extremely simplely moreover. The one-step sampling and sample homogenizing inactivation scheme enables the device to have more functions and more flexible use. If a specific sample preserving fluid such as a virus extinguishing agent is additionally added, the one-step inactivation and quantitative sample adding of the sampling sample can be realized; if the normal-temperature sample preservative is added, normal-temperature transportation, sample pretreatment and quantitative sample adding of the sampling sample can be realized.
Drawings
Fig. 1 shows a cross-sectional view of a sampling tube capable of quantitatively transferring a sample according to an embodiment of the present invention.
Fig. 2 is a structural diagram of a sampling tube capable of quantitatively transferring a sample according to an embodiment of the present invention.
Fig. 3 is a view showing a structure of a cap of a sampling tube capable of quantitatively transferring a sample according to another embodiment of the present invention.
Fig. 4 shows a cross-sectional view of a cap of a sampling tube capable of quantitatively transferring a sample according to another embodiment of the present invention.
Fig. 5 shows a bottom view of a cap of a sampling tube capable of quantitatively transferring a sample according to another embodiment of the present invention.
Fig. 6 is a schematic diagram of a top cap of a sampling tube capable of quantitatively transferring a sample according to another embodiment of the present invention.
Fig. 7 shows a cross-sectional view of a top cap of a sampling tube capable of quantitatively transferring a sample according to another embodiment of the present invention.
Description of the element reference numerals
1 tube cap
2 sample collecting hose
3 cover bottom
31 second liquid outlet
4 cover cap
41 first liquid outlet
5 sample transfer Chamber
6 Top cover
61 liquid leakage prevention inner plug
7 sampling member
Detailed Description
The following description is provided for illustrative purposes, and other advantages and features of the present invention will become apparent to those skilled in the art from the following detailed description.
Please refer to fig. 1-7. It should be understood that the structure, ratio, size and the like shown in the drawings attached to the present specification are only used for matching with the content disclosed in the specification, so as to be known and read by those skilled in the art, and are not used for limiting the limit conditions that the present invention can be implemented, so that the present invention has no technical essential meaning, and any structure modification, ratio relationship change or size adjustment should still fall within the scope that the technical content disclosed in the present invention can cover without affecting the function that the present invention can produce and the purpose that the present invention can achieve. Meanwhile, the terms such as "upper", "lower", "left", "right", "middle" and "one" used in the present specification are for convenience of description, and are not intended to limit the scope of the present invention, and changes or adjustments of the relative relationship thereof may be made without substantial technical changes, and the present invention is also regarded as the scope of the present invention.
As shown in fig. 1 and fig. 2, the sampling tube capable of quantitatively transferring a sample according to an embodiment of the present invention at least includes, from top to bottom: the tube cover 1 and the sample collection hose 2 are mutually covered, as shown in fig. 3, the tube cover 1 comprises a cover bottom 3 and a cover cap 4, as shown in fig. 4, the cover bottom 3 is covered with the sample collection hose 2, and the cover cap 4 is arranged above the cover bottom 3 and encloses a sample transfer cavity 5 with the cover bottom; the top of the cap 4 is provided with a first liquid outlet 41 communicated with the outside; the aperture of the first liquid outlet 41 is 1mm-5 mm; as shown in fig. 4 and 5, a second liquid outlet hole 31 for communicating the sample collection hose 2 with the sample transfer chamber is formed in the cover bottom 3; the tube cover 1 further comprises a top cover 6, and the top cover 6 can be detachably covered on the cap 4.
The sample collection hose can deform under the action of external force, and the inner cavity of the tube can flow by extruding the sample collection hose; and is pressed through the second exit opening 31 into the sample transfer chamber. And then is discharged out of the sampling tube through the first liquid outlet hole. The sample collection hose may be a plastic hose.
The interval sets up between sample transfer chamber and the sample collection hose, only goes out liquid hole intercommunication through the second, and must enter into the sample transfer chamber through the extrusion, has the cushioning effect, prevents that liquid from rocking and being stained with and cause the seepage etc. on first liquid hole of going out in transfer or transportation.
The volume of sample collection hose can set up as required, can set up and guarantee that sample volume control is between 500 ~ 5000 mL.
The aperture of the first liquid outlet 41 is 1mm-5 mm; the quantitative sample feeding device solves the problem of quantitative sample feeding without specialized equipment and opening a cover, namely, a quantitative transfer sample control system can just discharge homogenized samples with required volume, and the volume of the homogenized samples discharged by pressing is controlled to be 400 +/-10 microliter per 5 drops. For samples which are difficult to pretreat, such as samples of excrement, silt, sputum and the like, the sample pretreatment operation of the device is extremely simple, the bad experience of sample treatment is greatly reduced, the risk of exposure in unsafe environment is greatly reduced, and a large number of samples can be treated by a rapid one-step method; the built-in sample preservation solution can be used for stably preserving the homogenate sample at room temperature after virus inactivation and is also convenient for normal-temperature transportation of the sample.
Further, as shown in fig. 7, an inner liquid-leakage-proof plug 61 is disposed on the top cover 6, and the position and shape of the inner liquid-leakage-proof plug 61 are matched with the first liquid outlet 41. The first liquid outlet hole can be blocked by the liquid leakage prevention inner plug, so that the uniform and good sample can not overflow or seep in the transportation process.
The number of the second liquid outlet holes 31 is 1-8. Can be flexibly arranged according to the capacity of the sampling pipe, and is convenient for extruding liquid.
In one embodiment, as shown in fig. 1, a sampling member 7 is disposed in the lumen of the sample collection tube 2, and one end of the sampling member 7 is fixed to the cover bottom 3. And the sampling is convenient.
The sampling member may be a cotton swab or a sampling swab. Such as a flocked swab.
After the sample is quantitatively collected and the sample preservative is added, proper inversion oscillation is carried out, so that the cells adhered to the swab sample can be conveniently in a homogeneous state, virus inactivation can be carried out, and the stable state can be maintained at room temperature for a long time.
In one embodiment, the sample collection hose 2 and cap 1 are threaded. Preventing liquid leakage.
The diameter of the cover bottom 3 is larger than that of the top of the cover cap 4, and the side wall of the cover cap 4 extends towards the inner cavity of the cover cap in a shrinkage mode. Such that the sample transfer chamber is a cone chamber. The first liquid outlet hole is formed in the end portion of the cone. The liquid can be conveniently collected and then discharged out of the sampling pipe.
Optionally, as shown in fig. 6, the outer side wall of the top cover 6 is provided with anti-slip threads.
In one embodiment, the bottom of the lumen of the sample collection hose 2 is contracted and extended toward the inside of the lumen such that the diameter of the bottom of the lumen is smaller than the diameter of the lumen above the bottom of the lumen. The liquid can be collected conveniently, and the excessive liquid can be prevented from remaining at the bottom of the tube.
During the use, can be through unscrewing the sample collecting pipe after, utilize cotton swab or flocking swab in the collecting pipe to carry out sample acquisition, the sample of gathering is not limited to nasopharynx swab sample, oropharynx swab sample, excrement and urine, saliva, blood stain etc. after adding sample preservation liquid or virus inactivation preservation liquid in the collecting pipe, insert the swab sample collecting pipe with the swab sample again in, the tube cap of screwing, after 3 ~ 5 times of up-and-down reversal mixing, can carry out the normal atmospheric temperature transportation of sample, the efficiency that the transportation that does not need the cold chain can very big improvement sample transport, the cost is saved. During transportation, the sample is automatically homogenized in the preservation solution or automatically inactivated in the virus inactivating agent. After the sample is transported the check point, open the top cap immediately, extrude the body gently, can easily extrude the liquid of required volume, carry out subsequent inspection work, do not need extra latency, the quantitative application of sample also does not need whole tube cap, can avoid like this that the sample exposes "aerosol" that produces in the air or the safety risk to operating personnel production, and does not need professional equipment, uses extremely simplely.
In collecting or preserving a sample, a sample preserving fluid and a virus inactivating agent may be used. Specifically, guanidine hydrochloride and SDS (sodium dodecyl sulfate) can be used to inactivate viruses at normal temperature, and EDTA can be used as a chelating agent of metal ions and as a stabilizing agent of a buffer solution to eliminate the inhibition effect in downstream enzyme catalytic reaction caused by trace heavy metals. Sodium chloride and potassium chloride can be used for coordinating the balance of ions inside and outside the cell membrane in the sample to protect the cells, and a proper amount of Tris-HCl can ensure the stability of the pH value during storage and transportation in the sample and prevent the cells from being broken; the phosphate and the potassium chloride can reduce the damage effect of strong acid and strong alkali environment on cells in a sample under a certain proportion, thereby ensuring the stable state of the cells; the addition of special resistant substances can also organize the propagation of microorganisms, so that the sample can be more stably stored.
Example 1 nucleic acid extraction after quantitative collection of oropharyngeal swab samples
A. Collecting an oropharynx swab sample by using the sampling tube, and adding 1500mL of virus inactivation and sample preservation solution;
B. the mixture is inverted and mixed for 5 times to ensure that the swab sample and the sample preservation solution are fully mixed, and the sample is homogenized by a one-step method;
C. gently pressing the tube body, quantitatively dripping 5 drops of 'homogeneous liquid', quantitatively discharging 400 +/-10 microliter of homogeneous sample, and directly adding the homogeneous sample into the nucleic acid extraction reagent of the prepackage plate for nucleic acid extraction.
Example 2 quantitative collection of blood stain samples, nucleic acid extraction after 3-5 days of normal temperature transportation
In this embodiment accessible dipping in blood stain sample added corresponding sample preservation liquid, "homogenization" handles the sample to use sample normal atmospheric temperature to preserve, the sample can be preserved for more than 7 days at room temperature steadily, and the sample that transports is directly carried out nucleic acid extraction.
A. Collecting an oropharynx swab sample by using the sampling tube, and adding 1500mL of virus inactivation and sample preservation solution;
B. the mixture is inverted and mixed for 5 times to ensure that the swab sample and the sample preservation solution are fully mixed, and the sample is homogenized by a one-step method;
C. gently pressing the tube body, quantitatively dripping 2 drops of 'homogeneous liquid', quantitatively discharging 150 +/-10 microliter of homogeneous sample, and directly adding the homogeneous sample into the prepackage plate nucleic acid extraction reagent for nucleic acid extraction.
The above embodiments are merely illustrative of the principles and effects of the present invention, and are not to be construed as limiting the invention. Modifications and variations can be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which may be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.
Claims (8)
1. A sampling tube capable of quantitatively transferring a sample, the sampling tube at least comprising from top to bottom: the tube cover (1) and the sample collection hose (2) are mutually covered, the tube cover (1) comprises a cover bottom (3) and a cover cap (4), the cover bottom (3) is covered with the sample collection hose (2), and the cover cap (4) is arranged above the cover bottom (3) and encloses a sample transfer cavity (5) with the cover bottom; the top of the cover cap (4) is provided with a first liquid outlet hole (41) communicated with the outside; the aperture of the first liquid outlet (41) is 1mm-5 mm; a second liquid outlet hole (31) for communicating the sample collection hose (2) with the sample transfer cavity is formed in the cover bottom (3); the tube cover (1) further comprises a top cover (6), and the top cover (6) can be detachably covered on the cover cap (4).
2. The sampling tube according to claim 1, wherein the top cover (6) is provided with a liquid-proof inner plug (61), and the position and shape of the liquid-proof inner plug (61) are matched with the first liquid outlet hole (41).
3. The sampling tube according to claim 1, wherein the number of the second exit openings (31) is 1-8.
4. The sampling tube according to claim 1, wherein a sampling member (7) is disposed in the lumen of the sample collection tube (2), and one end of the sampling member (7) is fixed to the cover bottom (3).
5. The sampling tube according to claim 1, wherein the sample collection hose (2) and the tube cap (1) are screwed together.
6. A sample tube according to claim 1, wherein the diameter of the lid base (3) is larger than the diameter of the top of the cap (4), and the side wall of the cap (4) extends in a converging manner towards the inner cavity of the cap.
7. The sampling tube capable of quantitatively transferring a sample according to claim 1, wherein the outer side wall of the top cap (6) is provided with an anti-slip pattern.
8. A sampling tube according to claim 1, wherein the bottom of the lumen of the sample collection tube (2) is constricted and extended towards the inside of the lumen, so that the diameter of the bottom of the lumen is smaller than the diameter of the lumen above the bottom of the lumen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021600732.1U CN212925026U (en) | 2020-08-05 | 2020-08-05 | Sampling tube capable of quantitatively transferring samples |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021600732.1U CN212925026U (en) | 2020-08-05 | 2020-08-05 | Sampling tube capable of quantitatively transferring samples |
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| CN212925026U true CN212925026U (en) | 2021-04-09 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113373030A (en) * | 2021-04-28 | 2021-09-10 | 任楚宇 | Nucleic acid detection sample storage device with self-destruction function |
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2020
- 2020-08-05 CN CN202021600732.1U patent/CN212925026U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113373030A (en) * | 2021-04-28 | 2021-09-10 | 任楚宇 | Nucleic acid detection sample storage device with self-destruction function |
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