CN212800388U - Cell culture I125Automatic particle irradiation box - Google Patents

Abstract

The utility model relates to the field of biomedical equipment, a cell culture I is disclosed¹²⁵The automatic particle irradiation box comprises a box body and a box body¹²⁵The device comprises a particle tray, a culture dish tray, double slide rails, a transmission assembly and a PLC (programmable logic controller), wherein a first opening is formed in the bottom of the front side of a box body, and a second opening is formed in the side surface of the box body; i is¹²⁵The particle tray is slidably assembled at the first opening, and the culture dish tray is slidably assembled at the second opening and is positioned at I¹²⁵A cell culture dish is arranged above the particle plate; conveying applianceThe movable assembly is arranged in the box body, is in transmission connection with the culture dish tray and is used for pushing the culture dish tray out of the box body; the PLC controller is electrically connected with the transmission assembly to realize the purpose of pushing the tray out of the box body at regular time to stop the irradiation experiment. Can automatically control the time for pushing the culture dish tray out of the box body through a control program arranged in the PLC according to the actual conditions of the experiment, thereby realizing automatic control I¹²⁵The purpose of the particle irradiation time.

Description

Cell culture I125Automatic particle irradiation box

Technical Field

The utility model relates to a biomedical apparatus field, concretely relates to cell culture I¹²⁵And (4) automatically irradiating the box by the particles.

Background

Radiation therapy is one of the important methods of treating various malignancies, including both distance and brachytherapy. Teletherapy, i.e. external irradiation, is well known for its clinical application, therapeutic effect and defects; brachytherapy refers to the use of radioisotope technology for treatment within 5cm of, or even within, tumor tissue.

In brachytherapy, radionuclides I¹²⁵The application is wide, the dosage rate is low, the action time is long, and the treatment ratio can be increased; the radioactive source is close to the tumor, has no shielding, and has high local treatment dosage, so that the damage of normal tissues is obviously reduced: the continuous irradiation can damage the DNA double-strand break more completely and can obviously increase the killing effect of the tumor cells.

But at present, for radionuclide I¹²⁵The application of (A) is not very empirical, so that it relates to the radionuclide I¹²⁵The problems of radiotherapy remain to be investigated. Radiobiology of low dose rate radiation has been the focus of research, but of relevance I¹²⁵The radiation culture cells of the particles do not have special culture equipment, and are currently used by related national research institutions, are designed by Grey laboratories in 2001 and are specially used for research I¹²⁵The in vitro experiment model that the particle lasts and irradiates with low dose rate, but this model design is simple, can't conveniently adjust the mode that the particle was arranged, can't automatic control irradiation time.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model lies in: in view of the above I¹²⁵The particle lasts the problem that the mode that can't conveniently adjust the particle and arrange and unable automatic control irradiation time that exists when the external experiment model that low dose rate shone exists, the utility model provides a cell culture I¹²⁵ParticlesAn automatic irradiation box.

In order to solve the technical problem, the utility model provides a cell culture I¹²⁵A particle autoirradiation chamber comprising:

the bottom of the front surface of the box body is provided with a first opening, and the side surface of the box body is provided with a second opening;

I¹²⁵a particle tray slidably fitted at the first opening and provided with a particle tray placing table on which I is placed¹²⁵Particle disks of the formula I¹²⁵A first baffle plate capable of completely covering the first opening is arranged at the end part of the particle tray, which is positioned outside the box body;

a culture dish tray slidably fitted at the second opening and located at I¹²⁵A cell culture dish is placed above the particle tray, a second baffle capable of completely covering the second opening is arranged on one side of the culture dish tray outside the box body, a counterweight groove is arranged on the other side of the culture dish tray, and a counterweight block is placed in the counterweight groove;

the double-sliding rail is horizontally arranged in the box body in the length direction of the box body, and the bottom of the culture dish tray is provided with a sliding chute corresponding to the double-sliding rail;

the transmission assembly is arranged in the box body, is in transmission connection with the culture dish tray and is used for pushing the culture dish tray out of the box body;

the PLC is electrically connected with the transmission assembly, and the timing control of starting and stopping the transmission assembly is realized through an internal control program so as to realize that the culture dish tray is pushed out of the box body at regular time to stop the irradiation experiment.

Further, the transmission assembly includes:

the motor is arranged at the bottom of the box body and electrically connected with the PLC, and the output end of the motor is provided with a driving gear;

the transmission shaft is perpendicular to the double sliding rails, arranged on one side of the motor and in transmission connection with the driving gear, is provided with a transmission tooth structure corresponding to the driving gear, and two ends of the transmission shaft are respectively provided with a take-up pulley;

the group of pulleys are respectively arranged on two sides of the second opening;

and the traction rope is used for connecting the culture dish tray with the transmission shaft in a transmission way, one end of the traction rope is bound on one side of the counterweight groove far away from the second opening, and the other end of the traction rope is wound on the corresponding take-up pulley after penetrating through the pulley.

Further, a third baffle plate capable of completely covering the second opening is arranged on one side, close to the second baffle plate, of the counterweight groove.

Furthermore, a fourth baffle is arranged on one side of the second baffle corresponding to the second opening, and the fourth baffle can just block the second opening.

Further, the box body, the first baffle, the second baffle, the third baffle and the fourth baffle are all made of lead or metal materials with the equivalent lead of 1.7 mmPb.

Furthermore, a plurality of 90-degree corner-shaped air holes are formed in the box body.

Further, the bottom plate material of culture dish tray is organic glass.

Furthermore, a culture dish placing groove corresponding to the cell culture dish is arranged on the bottom plate of the culture dish tray.

Further, the I¹²⁵The particle disk is square, I thereon¹²⁵The particles are arranged according to an array of 4x4, and the particle plate placing table is provided with a particle plate I¹²⁵The particle disc placing groove corresponds to the particle disc, and the groove depth of the particle disc placing groove is smaller than I¹²⁵Thickness of the particle disk.

Further, the height of the particle tray placing table is adjustable.

Compared with the prior art, the utility model has the advantages of:

1. can automatically control the time for pushing the culture dish tray out of the box body through a control program arranged in the PLC according to the actual conditions of the experiment, thereby realizing automatic control I¹²⁵The purpose of the particle irradiation time;

2、I¹²⁵the particle tray is provided with a particle tray placing table with adjustable height, the irradiation height can be adjusted according to actual conditions, and I can be selected automatically¹²⁵Shape of particle disk and I¹²⁵The arrangement form of the particles is convenient and flexible.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments described in the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

Figure 1 is a schematic view of the overall structure of the utility model,

FIG. 2 shows a view of a table with particle trays 3¹²⁵A schematic view of the structure of the particle tray 2,

FIG. 3 is I¹²⁵ Particle disc 7 upper I¹²⁵The schematic diagram of the arrangement of the particles,

figure 4 is a schematic view of the structure of the culture dish tray 5,

figure 5 is a schematic view of the state of the whole irradiation chamber during irradiation,

fig. 6 is a schematic perspective view of the cabinet 1 with the front panel and the top panel removed, based on fig. 5, showing the internal structure of the cabinet 1,

figure 7 is a front view of figure 6,

fig. 8 is another perspective view of the cabinet 1 with the front panel and the top panel removed, focusing on the structure of the transmission assembly 9 in the cabinet 1,

FIG. 9 is a schematic view showing a state where the cell culture dish 6 is pushed out of the chamber 1 after irradiation is completed with the front panel and the top plate of the chamber 1 removed,

figure 10 is a front view of figure 9,

fig. 11 is a partial enlarged view of i in fig. 9, showing the structure of the 90 deg. corner type airing hole 13,

the reference numbers are as follows:

the box body 1, a first opening 11, a second opening 12, an air hole 13, I¹²⁵ Particle tray 2, first baffle 21, particle tray table 3, particle tray placement groove 31, I¹²⁵A particle tray 4, a culture dish tray 5, a second baffle 51, a counterweight groove 52, a sliding groove 53 and a third baffle54, fourth baffle 55, culture dish standing groove 56, cell culture dish 6, balancing weight 7, two slide rails 8, drive assembly 9, motor 91, driving gear 92, transmission shaft 93, take-up pulley 94, pulley 95, haulage rope 96, PLC controller 10.

Detailed Description

In order to make the technical solution of the present invention better understood, the present invention is described in detail below with reference to the accompanying drawings, and the description of the present invention is only exemplary and explanatory, and should not be construed as limiting the scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

It should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like refer to the orientation or positional relationship shown in the drawings, or the orientation or positional relationship that the utility model is usually placed when in use, and are used for convenience of description and simplification of description, but do not refer to or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal", "vertical", "overhang" and the like do not imply that the components are required to be absolutely horizontal or overhang, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to FIGS. 1 to 10, the present invention provides a cell culture system I¹²⁵The automatic particle irradiation box comprises a box body 1 and a box body I¹²⁵The device comprises a particle tray 2, a culture dish tray 5, a double slide rail 8, a transmission assembly 9 and a PLC (programmable logic controller) 10;

as shown in fig. 1, the bottom of the front surface of the box body 1 is provided with a first opening 11, and the side surface of the box body 1 is provided with a second opening 12, so as to reduce the radiation of particles to the external environment and ensure the safety, the box body 1 should be made of a radiation-proof material, preferably lead, or other metal materials with equivalent lead of 1.7 mmPb;

I¹²⁵the particle tray 2 is slidably mounted at the first opening 11, a particle tray placing table 3 is provided thereon, and I is placed on the particle tray placing table 3¹²⁵ Particle disks 4, I¹²⁵The end of the particle tray 2 outside the box 1 is provided with a first baffle 21 that can completely cover the first opening 11, as shown in fig. 5, specifically: the first baffle 21 is larger than the first opening 11, so that the first opening 11 can be completely covered, and I is placed in the experiment process¹²⁵Particle disk table 3 for particle disks 4, pass I¹²⁵When the particle tray 2 is pushed into the box body 1 for irradiation, the first opening 11 can be completely blocked from the outer side of the box body 1 through the first baffle 21 to prevent radiation leakage, so that the experimental safety is ensured;

the culture dish tray 5 is slidably mounted at the second opening 12 and is located at I¹²⁵The top of particle dish 4, the bottom plate material of culture dish tray 5 are organic glass, have placed cell culture dish 6 on it, and one side that culture dish tray 5 is located the box 1 outside is equipped with the second baffle 51 that can cover second opening 12 completely, as figure 6 and figure 7, specifically do: the second baffle 51 is larger than the second opening 12, and during the experiment, the culture dish tray 5 with the cell culture dish 6 is pushed into the box body 1 for irradiation experimentIn the process, the second baffle 51 can be arranged to completely block the second opening 12 from the outer side of the box body 1 to prevent radiation leakage and ensure the safety of the experiment;

as shown in fig. 4, 6 and 8, the other side of the culture dish tray 5 is provided with a counterweight groove 52, and the counterweight groove 52 is provided with a counterweight 7, so that the culture dish tray 5 can keep balance without being supported outside after the cell culture dish 6 is pushed out of the box body 1;

as shown in fig. 4 and 10, in order to prevent radiation leakage from inside the case 1 when the cell culture dish 6 is completely pushed out of the case 1, a third barrier 54 capable of completely covering the second opening 12 is provided on the side of the weighing groove 52 adjacent to the second barrier 51, so that the second opening 12 is completely blocked by the third barrier 54 provided after the cell culture dish 6 is pushed out of the case 1, thereby preventing radiation leakage;

furthermore, a fourth baffle 55 is arranged on one side of the second baffle 51 corresponding to the second opening 12, the fourth baffle 55 can just block the second opening 12, the size of the fourth baffle 55 is equivalent to that of the second opening 12, and after the culture dish tray 5 is pushed into the box body 1, the fourth baffle 55 can just seal the second opening 12, so that radiation leakage can be further prevented;

the first baffle 21, the second baffle 51, the third baffle 54 and the fourth baffle 55 should be made of lead or a metal material with an equivalent lead of 1.7 mmPb;

as shown in FIG. 7, when the culture dish tray 5 is irradiated into the housing 1, the position of the cell culture dish 6 placed on the culture dish tray 5 should be exactly at I¹²⁵For convenience of positioning, a petri dish placing groove 56 should be arranged on the bottom plate of the petri dish tray 5 in design, as shown in fig. 4, and a particle dish placing groove 31 is arranged on the particle dish placing table 3, as shown in fig. 2, when the petri dish tray 5 enters the box body 1 for irradiation experiment, the petri dish placing groove 56 should be located just above the particle dish placing groove 31;

note that, for convenience, the cell culture dishes 6 and I¹²⁵The taking of the particle plate 4, the depth of the culture dish placing groove 56 should be smaller than the height of the cell culture dish 6The depth of the particle tray placing groove 31 should be smaller than I¹²⁵The thickness of the particle disk 4;

the double slide rails 8 are horizontally arranged in the box body 1 in the length direction of the box body 1, sliding grooves 53 corresponding to the double slide rails 8 are formed in the bottom of the culture dish tray 5, and the culture dish tray 5 is assembled on the double slide rails 8 in a sliding mode through the sliding grooves 53;

the transmission assembly 9 is arranged inside the box body 1, is in transmission connection with the culture dish tray 5 and is used for pushing the culture dish tray 5 out of the box body 1;

as shown in fig. 8, as a preferred solution, the transmission assembly 9 includes a motor 91, a transmission shaft 93, a set of pulleys 95 and a traction rope 96;

wherein, the motor 91 is arranged at the bottom of the box body 1, is electrically connected with the PLC 10, and the output end thereof is provided with a driving gear 92;

the transmission shaft 93 is arranged perpendicular to the direction of the double slide rails 8, is positioned on one side of the motor 91 and is in transmission connection with the driving gear 92, a transmission gear structure corresponding to the driving gear 92 is arranged on the transmission shaft 93, and two ends of the transmission shaft 93 are respectively provided with a take-up pulley 94;

pulleys 95 are respectively provided on both sides of the second opening 12;

the pulling rope 96 is used for connecting the culture dish tray 5 and the transmission shaft 93 together in a transmission way, one end of the pulling rope is fixed on one side of the counterweight groove 52 far away from the second opening 12, and the other end of the pulling rope passes through the pulley 95 and then is wound on the corresponding take-up pulley 94;

the motor 91 provides power, the driving gear 92 drives the transmission shaft 93 to rotate, the take-up pulleys 94 positioned at two ends of the transmission shaft 93 rotate along with the rotation, and the traction rope 96 wound on the take-up pulleys 94 is continuously tightened, so that the traction rope 96 generates a pulling force and pushes the culture dish tray 5 out of the box body 1 along the direction of the double slide rails 8, and when the third baffle 54 abuts against the second opening 12, the motor 91 stops;

to prevent I¹²⁵The radiation influence of the particle disk 4 even damages the motor 91 arranged in the box body 1, a radiation-proof protective cover can be arranged outside the motor 91, for example, the above lead or other metal materials with equivalent lead of 1.7mmPb can be arranged, the specific structural form can be designed according to the actual situation, and the specific structural form is not limited herein;

of course, other transmission mechanisms can be adopted to replace the preferred scheme, and the design can be specifically designed according to the actual requirement;

the PLC 10 is electrically connected with the transmission assembly 9, the timing control of starting and stopping the transmission assembly 9 is realized through a built-in control program, so that the culture dish tray 5 is pushed out of the box body 1 at regular time to stop the irradiation experiment, and in the specific experiment process, an experimenter can set time according to the actual situation;

it should be noted that, in order to accurately control the downtime of the motor 91, an induction patch may be disposed on the third baffle 54, the induction patch is electrically connected to the PLC controller 10, when the induction patch is supported on the inner side of the box 1, a signal is transmitted to the PLC controller 10, and then the motor is controlled to be shut down by the PLC controller 10, and control programs in the PLC controller 10 are conventional prior art, and are not described herein again.

Preferably, as shown in FIG. 3, I¹²⁵The particle disk 4 is square, I¹²⁵I on the particle disk 4¹²⁵The particles are arranged according to an array of 4x4, and the experimenter can select I according to the actual situation¹²⁵Arrangement of the particles.

The height of the particle disc placing table 3 can be adjusted, the flexibility of an experiment can be improved by the arrangement, as shown in fig. 2, a plurality of supporting legs with adjustable height are arranged at the bottom of the particle disc placing table 3, and the structure with adjustable height can be designed automatically.

As shown in fig. 10 and 11, the radiation trajectory of the low dose rate radiation-based rays is a straight line and cannot be turned, and in order to realize ventilation of the box body 1 and avoid radiation leakage, a plurality of 90-degree corner-shaped ventilation holes 13 are formed in the box body 1.

The experimenter can carry out daily internal maintenance and overhaul work on the whole irradiation box through the first opening 11.

The working principle is as follows: as shown in FIGS. 6 and 7, before starting the irradiation experiment, I is first placed¹²⁵Particle disk table 3 of particle disk 4 passes through I¹²⁵The particle tray 2 is pushed out of the box and the first baffle 21 is made to seal the first opening 11 and then the fine particles are placedThe culture dish tray 5 of the cell culture dish 6 is pushed into the box body 1 to carry out an irradiation experiment, and the irradiation time is set on the PLC 10;

after the irradiation time is reached, the PLC 10 controls the motor 91 to start, the transmission shaft 93 is driven to rotate, the take-up pulley 94 positioned at two ends of the transmission shaft 93 rotates, the traction rope 96 wound on the take-up pulley 94 is continuously tightened, the traction rope 96 generates a pulling force to push the culture dish tray 5 out of the box body 1 along the direction of the double-slide rail 8, the motor 91 is stopped when the third baffle 54 abuts against the second opening 12, and the irradiation is finished, such as 9 and 10.

The utility model discloses a PLC controller 10 is to the timing control of motor 91, realized regularly outside releasing box 1 with cell culture dish 6 to realize automatic control irradiation time.

The foregoing is a more detailed description of the present invention, taken in conjunction with the specific preferred embodiments thereof, and it is not intended that the invention be limited to the specific embodiments shown and described. To the utility model belongs to the technical field of ordinary technical personnel, do not deviate from the utility model discloses under the prerequisite of design, can also make a plurality of simple deductions or replacement, all should regard as belonging to the utility model discloses a protection scope.

Claims (10)

1. Cell culture I¹²⁵Particle automatic irradiation case, its characterized in that includes:

the box body (1) is provided with a first opening (11) at the bottom of the front surface and a second opening (12) at the side surface;

I¹²⁵a particle tray (2) which is slidably assembled at the first opening (11) and is provided with a particle tray placing table (3), wherein I is placed on the particle tray placing table (3)¹²⁵Particle disks (4), the said I¹²⁵A first baffle (21) capable of completely covering the first opening (11) is arranged at the end part of the particle tray (2) positioned outside the box body (1);

a culture dish tray (5) slidably mounted at the second opening (12) and located at I¹²⁵A cell culture dish (6) is placed above the particle tray (4), and a second baffle plate capable of completely covering the second opening (12) is arranged on one side of the culture dish tray (5) outside the box body (1)(51) The other side of the box body is provided with a counterweight groove (52), and a counterweight block (7) is placed in the counterweight groove (52);

the double-sliding rail (8) is horizontally arranged in the box body (1) in the length direction of the box body (1), and a sliding groove (53) corresponding to the double-sliding rail (8) is formed in the bottom of the culture dish tray (5);

the transmission assembly (9) is arranged inside the box body (1), is in transmission connection with the culture dish tray (5) and is used for pushing the culture dish tray (5) out of the box body (1);

and the PLC (10) is electrically connected with the transmission assembly (9), and realizes the timing control of starting and stopping the transmission assembly (9) through an internal control program so as to realize the timing of pushing the culture dish tray (5) out of the box body (1) to stop the irradiation experiment.

2. Cell culture I according to claim 1¹²⁵Automatic particle irradiation box, characterized in that said transmission assembly (9) comprises:

the motor (91) is arranged at the bottom of the box body (1) and is electrically connected with the PLC (10), and the output end of the motor is provided with a driving gear (92);

the transmission shaft (93) is perpendicular to the double sliding rails (8), arranged on one side of the motor (91) and in transmission connection with the driving gear (92), a transmission gear structure corresponding to the driving gear (92) is arranged on the transmission shaft, and two ends of the transmission shaft (93) are respectively provided with a take-up pulley (94);

a set of pulleys (95) respectively arranged at two sides of the second opening (12);

and the traction rope (96) is used for connecting the culture dish tray (5) with the transmission shaft (93) in a transmission way, one end of the traction rope is bound on one side of the counterweight groove (52) far away from the second opening (12), and the other end of the traction rope penetrates through the pulley (95) and then is wound on the corresponding take-up pulley (94).

3. Cell culture I according to claim 1¹²⁵Automatic irradiation case of particle, its characterized in that: and a third baffle plate (54) capable of completely covering the second opening (12) is arranged on one side of the counterweight groove (52) close to the second baffle plate (51).

4. The cell of claim 3Culture I¹²⁵Automatic irradiation case of particle, its characterized in that: and a fourth baffle (55) is arranged on one side of the second baffle (51) corresponding to the second opening (12), and the fourth baffle (55) can just block the second opening (12).

5. The cell culture of claim 4I¹²⁵Automatic irradiation case of particle, its characterized in that: the box body (1), the first baffle (21), the second baffle (51), the third baffle (54) and the fourth baffle (55) are all made of lead or metal materials with the equivalent lead of 1.7 mmPb.

6. Cell culture I according to claim 1¹²⁵Automatic irradiation case of particle, its characterized in that: the box body (1) is provided with a plurality of 90-degree corner-shaped air holes (13).

7. Cell culture I according to claim 1¹²⁵Automatic irradiation case of particle, its characterized in that: the bottom plate of the culture dish tray (5) is made of organic glass.

8. Cell culture I according to claim 1¹²⁵Automatic irradiation case of particle, its characterized in that: and a culture dish placing groove (56) corresponding to the cell culture dish (6) is arranged on the bottom plate of the culture dish tray (5).

9. Cell culture I according to claim 1¹²⁵Automatic irradiation case of particle, its characterized in that: said I¹²⁵The particle disk (4) is square, I on it¹²⁵The particles are arranged according to an array of 4x4, and the particle tray placing table (3) is provided with a particle tray I¹²⁵A particle disc placing groove (31) corresponding to the particle disc (4), wherein the groove depth of the particle disc placing groove (31) is less than I¹²⁵Thickness of the particle disk (4).

10. Cell culture I according to claim 1¹²⁵Automatic irradiation case of particle, its characterized in that: the height of the particle disc placing table (3) can be adjusted.

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