CN212770777U - External filtering passageway scar model device based on micro-fluidic system - Google Patents
External filtering passageway scar model device based on micro-fluidic system Download PDFInfo
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Abstract
The utility model relates to an external passageway scar model device of straining based on micro-fluidic system. The in vitro filtering aisle scar model device comprises a middle layer plate, wherein a strip-shaped through groove which is arranged in a vertically through manner is arranged on the middle layer plate, and circular through grooves are arranged in the middle and/or two ends of the strip-shaped through groove; the bottom plate is used for supporting the middle plate, and the elongated through grooves and the circular through grooves of the middle plate are matched to enclose a simulated liquid flow channel and a cell suspension storage cavity; the upper plate is connected with the middle plate in a sealing way; two through holes are respectively formed in the upper plate corresponding to two ends of the strip-shaped through groove; the bottom layer plate, the middle layer plate and the upper layer plate are pre-pressed and integrally formed. This external filtration passageway scar model device adopts the integrated cell culture device of three laminar formulas, including pre-compaction integrated into one piece's three-layer plate structure, its inside rectangular shape leads to the groove can conveniently simulate liquid and flow, forms one-way flow to the cell suspension that circular logical inslot erodees, practices thrift the cell, is convenient for filter fast.
Description
Technical Field
The utility model relates to an external passageway scar model device of straining based on micro-fluidic system.
Background
According to the latest statistics of the world health organization, there are currently up to 66.000.000 glaucoma patients worldwide, and 12.500.000 patients are blinded by glaucoma. Glaucoma is a blinding eye disease characterized by optic nerve damage and visual field loss, the resulting impairment of visual function being irreversible. Thus, glaucomatologists recognize glaucoma as an irreversible and unpredictable disease; the consequences are extremely severe, causing immeasurable pain and loss to individuals, families and society. Under current medical conditions, the majority of glaucoma patients eventually inevitably require surgical treatment to control the progression of the disease.
Glaucoma trabeculectomy is performed by establishing an aqueous humor filtering channel from an anterior chamber to a scleral surface in an eye to form a filtering bubble under a fascia sac, so that the aims of external drainage of aqueous humor and intraocular pressure reduction are fulfilled (fig. 1). The most significant factor leading to trabeculectomy failure is post-operative bleb scarring, with the most significant areas of scarring lying between the scleral and Tenon's fascial surfaces. The abnormal proliferation and migration of fibroblasts is the basis of scar formation, so that the research on the fibroblasts (HTFs) from the Tenon bursa has important significance for regulating and controlling the scar formation of the filtering bleb after the trabeculectomy.
The current study on anti-scarring of the bleb after glaucoma trabeculectomy has certain limitations. Based on the research of in vitro level, the ordinary cell culture can not simulate the dynamic environment of aqueous humor flow circulation, and the fluid field interferes with various cell surface signal paths, so that the static cell culture can not completely reflect the functions of filtering bubble HTFs; the research based on the in vivo model, no matter human body or animal, is difficult to realize the direct observation of the flow of the aqueous humor of the filtration channel widely, and particularly human body experiments are not developed due to the fact that a plurality of unexplained ethical problems are faced.
In view of the above problems, an in vitro simulation device convenient for the above research and observation is developed, and the microfluidic chip system is utilized to simulate the unidirectional outflow of aqueous humor, thereby facilitating the observation of the biological functions of HTFs and the change of cell signal paths in a fluid field.
SUMMERY OF THE UTILITY MODEL
The utility model provides an in vitro filtration passageway scar model device based on micro-fluidic system adopts the integrated cell culture device of three laminars, including pre-compaction integrated into one piece's three-layer plate structure, its inside rectangular shape leads to the groove can conveniently simulate the liquid and flow, and cell suspension to circular logical inslot forms the unidirectional flow and erodees, can simulate the biological characteristics of glaucoma surgery back room water unilaterally drainage, can practice thrift the cell again, and the problem that exists among the prior art has been solved to the quick screening of being convenient for.
The utility model discloses a solve the technical scheme that above-mentioned technical problem adopted and be:
an in vitro aisle scar model filtering device based on a microfluidic system comprises:
the middle layer plate is provided with a strip-shaped through groove which is arranged in a vertically through manner, and the middle part and/or two ends of the strip-shaped through groove are/is provided with round through grooves;
the bottom plate is used for supporting the middle plate, and the elongated through grooves and the circular through grooves of the middle plate are matched to enclose a simulated liquid flow channel and a cell suspension storage cavity;
the upper plate is connected with the middle plate in a sealing way; two through holes are respectively formed in the upper plate corresponding to two ends of the strip-shaped through groove;
the bottom layer plate, the middle layer plate and the upper layer plate are pre-pressed and integrally formed.
Further, the bottom layer plate is fixedly connected with the middle layer plate; the upper plate is detachably connected with the middle plate in a sealing manner.
Further, the middle layer plate and the bottom layer plate are both polymethyl methacrylate (PMMA) plates, and the upper layer plate is a medical soft plate.
Furthermore, the medical soft plate is a silica gel plate.
Further, the circular through groove is formed in the middle of the strip-shaped through groove of the middle layer plate and used for containing cell suspension; the two through holes are connected with infusion catheters, and one of the infusion catheters is connected with a microfluidic system.
Further, the microfluidic system comprises a syringe pump; through the flow and the velocity of flow of the simulation liquid of control syringe pump control by the syringe pump notes out to it is mobile to be closer internal aqueous humor, realizes the utility model discloses the analog state of device.
Further, the round through grooves are symmetrically arranged at the two ends of the strip-shaped through groove close to the middle layer plate; an exhaust pipeline is communicated with the middle of the strip-shaped through groove, the gas outlet of the exhaust pipeline extends out of the side wall of the middle layer plate, and a plug cover is arranged at the end part of the gas outlet of the exhaust pipeline.
Furthermore, the round through grooves are respectively arranged at the two ends close to the strip-shaped through groove.
Furthermore, two or more circular through grooves are arranged at the positions, close to the two ends of the long strip-shaped through groove, of the two ends of the circular through groove respectively, and the circular through groove is communicated with the long strip-shaped through groove through an inclined communicating pipe.
Furthermore, the width of the strip-shaped through groove is 2mm, and the diameter of the circular through groove is 5 mm.
The utility model has the advantages that:
this external passageway scar model device of straining based on micro-fluidic system adopts the integrated cell culture device of three laminars, including prepressing integrated into one piece's three-layer plate structure: bottom plate, middle plywood and top plate, simple structure, convenient to use, especially the setting of upper silica gel board, the closed simulation passageway that compares two current laminar PMMA substrates and form can only collect discharge liquid and observe, the utility model discloses a can open upper silica gel board after the simulation is ended, continue to carry out experiments such as cell fluorescence dyeing in the passageway, provide further function for filtering bubble scar formation research. In addition, the circular through groove of the device is used for culturing cell suspension, so that cells are saved, and the research on a forming mechanism of filtering bleb scars or the rapid screening of scar forming medicines is more convenient to regulate and control.
Drawings
FIG. 1 is a schematic view of aqueous humor outflow after trabeculectomy;
fig. 2 is a schematic structural diagram of embodiment 1 of the present invention;
FIG. 3 is a schematic top view of the structure of FIG. 2;
fig. 4 is a reference diagram of the usage status of embodiment 1 of the present invention;
fig. 5 is a schematic structural diagram of embodiment 2 of the present invention;
FIG. 6 is a schematic top view of the structure of FIG. 5;
fig. 7 is a reference diagram of the usage status of embodiment 2 of the present invention;
fig. 8 is a schematic structural view of embodiment 3 of the present invention;
FIG. 9 is a schematic top view of the structure of FIG. 8;
fig. 10 is a reference diagram of the usage status of embodiment 3 of the present invention;
fig. 11 is a schematic top view of fig. 10.
Wherein, 1 bottom plate, 2 middle plate, 3 upper plate, 4 rectangular through grooves, 5 circular through grooves, 6 liquid inlet holes, 7 liquid outlet holes, 8 liquid conveying pipes, 9 exhaust pipelines, 10 plugs and 11 inclined communicating pipes.
Detailed Description
In order to clearly illustrate the technical features of the present solution, the present invention is explained in detail through the following embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Example 1
As shown in fig. 2-4, the scar model device for in vitro filtering passageway based on the microfluidic system comprises a bottom plate 1, a middle plate 2 and an upper plate 3 which are sequentially arranged from bottom to top, wherein the bottom plate, the middle plate and the upper plate are arranged by pre-pressing and integral forming, the bottom plate and the middle plate are both PMMA plates, the upper plate is a silica gel plate, the silica gel plate and the middle plate can be detachably connected in a sealing manner, the detachable structure is that the silica gel plate can be directly sealed and pressed on the middle plate, and the upper silica gel plate can be opened at the later stage to expose the middle plate.
A strip-shaped through groove 4 which is arranged in a penetrating manner up and down is arranged on the middle layer plate, and a round through groove 5 is arranged in the middle of the strip-shaped through groove; the depth of the strip-shaped through groove and the depth of the round through groove on the middle layer plate are consistent, the outline of the round through groove is larger than the width of the strip-shaped through groove, namely the width of the strip-shaped through groove is set to be 2mm, and the inner diameter of the round through groove is set to be 5 mm.
The bottom plate is mainly used for supporting the middle plate, and the strip-shaped through groove 4 and the round through groove 5 of the middle plate are matched to form a simulated liquid flow channel and a cell suspension storage cavity. The upper layer plate 3 is connected with the middle layer plate 2 in a sealing way; two through holes are respectively formed in the two ends, corresponding to the long-strip-shaped through grooves, of the upper plate, the through hole in the left end of the upper plate is a liquid inlet hole 6, the through hole in the right end of the upper plate is a liquid outlet hole 7, and liquid conveying pipes 8 are connected to the liquid inlet hole and the liquid outlet hole. The liquid inlet end of the liquid conveying pipe connected with the liquid inlet hole is connected with an injection pump of the micro-fluidic system, which is not shown in the figure. The flow and the velocity of flow of the simulation liquid of pouring out through the control syringe pump to the simulation is more close internal aqueous humor and flows, realizes the utility model discloses the analog state of device.
When the device is used, the cell suspension is filled through the liquid inlet hole 6 corresponding to one end of the strip-shaped channel 4 through the liquid conveying pipe, so that the cell suspension enters the circular through groove 5, and the filling quantity is stopped. Treat cell adherent growth, the transfer line 8 that links to each other with feed liquor hole 6 links to each other with micro-fluidic system's syringe pump, injection speed and the injection volume of control simulation liquid through micro-fluidic system, simulation liquid gets into by the feed liquor hole 6 of rectangular shape passageway 4 gradually, along the one-way flow of rectangular shape passageway, flow through the cell on the wall, the one-way flow effect of simulation aqueous humor, finally discharge to the transfer line 8 that links to each other with play liquid hole 7 by play liquid hole 7 and discharge, be convenient for observe the filtration bubble scar formation condition during, follow-up can carry out further research to the formation of filtration bubble scar. If the transfusion tube 8 connected with the liquid inlet hole 6 and the liquid outlet hole 7 is detached after the simulation liquid one-way simulation flow is finished, the silica gel plate of the upper plate 5 is taken down from the device, the simulation channel and the cells passing through the simulation liquid flow in the simulation channel are exposed at the moment, and the filtering bubble scar forming condition can be further researched through experiments such as immunofluorescence and the like.
Example 2
As shown in fig. 5-7, the in vitro filtration channel scar model device based on the microfluidic system is the same as the in vitro filtration channel scar model device of embodiment 1, except that the circular through grooves 5 are arranged at two ends of the elongated through groove 4 close to the middle plate 2, and the two circular through grooves are symmetrically arranged; an exhaust pipeline 9 is communicated with the middle part of the strip-shaped through groove, an air outlet of the exhaust pipeline extends out of the side wall of the middle layer plate, and a plug cover 10 is arranged at the end part of the air outlet of the exhaust pipeline.
During the use, fill the same or different cell suspensions to two circular logical inslots respectively simultaneously through feed liquor hole 6 and play liquid hole 7 earlier, in the filling process, gag 10 is in the open mode, after filling suitable volume, stop filling, close the gag, after cell adherent growth, link to each other with infusion pipe 8 that feed liquor hole 6 links to each other with the syringe pump, carry out the flow of simulation liquid in this analogue means's one-way runner, the simulation liquid is discharged by the transfer pipe 8 that links to each other with play liquid hole 7, the filtration bubble scar formation condition is observed to the while.
Example 3
As shown in fig. 8 to 11, the in vitro filtration corridor scar model device based on the microfluidic system is the same as the in vitro filtration corridor scar model device of embodiment 2, except that the number of the circular through grooves at the two ends of the elongated through groove 4 close to the middle plate 2 is two, which are symmetrically arranged at the two sides of the end of the elongated through groove, and the two circular through grooves are communicated through the inclined communicating pipe 11 which is obliquely arranged with the elongated through groove.
The use process refers to the use process of example 2 and is not described in detail here. Through setting up a plurality of circular logical grooves, can realize increasing the effect that different cell types carried out the simulation, use more in a flexible way, it is convenient.
The above-mentioned specific embodiments can not be regarded as the restriction to the scope of protection of the utility model, to technical personnel in this technical field, it is right the utility model discloses any replacement improvement or transform that embodiment made all fall within the scope of protection of the utility model.
The parts of the present invention not described in detail are the known techniques of those skilled in the art.
Claims (9)
1. An in vitro filter channel scar model device based on a microfluidic system is characterized by comprising:
the middle layer plate is provided with a strip-shaped through groove which is arranged in a vertically through manner, and the middle part and/or two ends of the strip-shaped through groove are/is provided with round through grooves;
the bottom plate is used for supporting the middle plate, and the elongated through grooves and the circular through grooves of the middle plate are matched to enclose a simulated liquid flow channel and a cell suspension storage cavity;
the upper plate is connected with the middle plate in a sealing way; two through holes are respectively formed in the upper plate corresponding to two ends of the strip-shaped through groove;
the bottom layer plate, the middle layer plate and the upper layer plate are pre-pressed and integrally formed.
2. The in vitro filtration channel scar model device based on the microfluidic system of claim 1, wherein the bottom plate and the middle plate are fixedly connected; the upper plate is detachably connected with the middle plate in a sealing manner.
3. The in vitro filter channel scar model device based on the microfluidic system of claim 1, wherein the middle layer plate and the bottom layer plate are both polymethyl methacrylate plates, and the upper layer plate is a medical soft plate.
4. The in vitro filter tract scar model device based on the microfluidic system as claimed in claim 3, wherein the medical soft plate is a silica gel plate.
5. The in vitro filter tunnel scar model device based on the microfluidic system according to any one of claims 1 to 4, wherein the circular through groove is arranged in the middle of the long strip-shaped through groove of the middle layer plate and is used for accommodating cell suspension; the two through holes are connected with infusion catheters, and one of the infusion catheters is connected with a microfluidic system.
6. The in vitro filter tunnel scar model device based on the microfluidic system as claimed in any one of claims 1 to 4, wherein the circular through grooves are symmetrically arranged at two ends of the long strip-shaped through groove close to the middle layer plate; an exhaust pipeline is communicated with the middle of the strip-shaped through groove, the gas outlet of the exhaust pipeline extends out of the side wall of the middle layer plate, and a plug cover is arranged at the end part of the gas outlet of the exhaust pipeline.
7. The in vitro filter tunnel scar model device based on the microfluidic system as claimed in claim 6, wherein one circular through groove is disposed near each end of the elongated through groove.
8. The in vitro filter channel scar model device based on the microfluidic system as claimed in claim 6, wherein two or more circular through grooves are respectively arranged at two ends of the elongated through groove, and the circular through grooves are communicated with the elongated through groove through inclined communicating pipes.
9. An in vitro filter scar model device according to any of claims 1 to 4 based on microfluidic systems, characterized by the elongated through slots having a width of 2mm and the circular through slots having a diameter of 5 mm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202021541704.7U CN212770777U (en) | 2020-07-30 | 2020-07-30 | External filtering passageway scar model device based on micro-fluidic system |
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| CN202021541704.7U CN212770777U (en) | 2020-07-30 | 2020-07-30 | External filtering passageway scar model device based on micro-fluidic system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114369532A (en) * | 2021-12-31 | 2022-04-19 | 中国科学院苏州纳米技术与纳米仿生研究所 | Micro-fluidic cell culture device for simulating filtering bubbles and application thereof |
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2020
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114369532A (en) * | 2021-12-31 | 2022-04-19 | 中国科学院苏州纳米技术与纳米仿生研究所 | Micro-fluidic cell culture device for simulating filtering bubbles and application thereof |
| CN114369532B (en) * | 2021-12-31 | 2023-10-03 | 中国科学院苏州纳米技术与纳米仿生研究所 | Microfluidic cell culture device simulating filtration bleb and application thereof |
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