CN214142391U - Micro-fluidic device for cell culture - Google Patents
Micro-fluidic device for cell culture Download PDFInfo
- Publication number
- CN214142391U CN214142391U CN202022470081.5U CN202022470081U CN214142391U CN 214142391 U CN214142391 U CN 214142391U CN 202022470081 U CN202022470081 U CN 202022470081U CN 214142391 U CN214142391 U CN 214142391U
- Authority
- CN
- China
- Prior art keywords
- cell
- cover plate
- middle diaphragm
- cell culture
- simulation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 26
- 239000012528 membrane Substances 0.000 claims abstract description 24
- 238000001914 filtration Methods 0.000 claims abstract description 15
- 238000004088 simulation Methods 0.000 claims description 32
- 238000007789 sealing Methods 0.000 claims description 9
- 239000001963 growth medium Substances 0.000 abstract description 11
- 239000002609 medium Substances 0.000 abstract description 11
- 210000004027 cell Anatomy 0.000 description 30
- 230000010261 cell growth Effects 0.000 description 5
- 238000001727 in vivo Methods 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 238000004891 communication Methods 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000003255 drug test Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000013327 media filtration Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a micro-fluidic device for cell culture, including film, middle diaphragm and cover plate, the film upside is provided with middle diaphragm, middle diaphragm upside is provided with the cover plate, middle diaphragm one end upside center is provided with the feed liquor hole, middle diaphragm other end upside center is provided with out the liquid hole, middle diaphragm center is embedded to have the cell cavity, cell cavity symmetry both sides are provided with medium filtration membrane. Has the advantages that: the utility model discloses a medium filtration membrane that sets up for the micro-fluidic device is inside to carry out the selectivity to the culture medium and filter, need not go on through external equipment, reduces the input of fund.
Description
Technical Field
The utility model relates to a cell culture field, concretely relates to micro-fluidic equipment for cell culture.
Background
Cells cultured on flat cell culture vessels typically have artificial two-dimensional cell sheets that can have morphologies and functions that are significantly different from cells cultured in vivo. Cultured cells are important for the discovery and development of modern drugs and are widely used for drug testing. However, if the results of such tests fail to show a response of the cells in vivo, the correlation of these results is reduced. Cells in the human body are in a three-dimensional environment completely surrounded by other cells, membranes, fibrous layers, adhesive proteins, and the like. Therefore, there is a need for a cell culture apparatus that better mimics in vivo conditions and promotes cultured cells with a morphology and function similar to those in vivo.
When the existing microfluidic device for cell culture is used, a medium filtering membrane is not generally arranged inside a microfluidic chip, a culture medium is filtered through the external filtering device, the investment of external equipment is increased, and therefore the problem existing in the prior art is solved.
SUMMERY OF THE UTILITY MODEL
Technical problem to be solved
The micro-fluidic chip aims to solve the problem that when the existing micro-fluidic device for cell culture is used, a medium filtering membrane is not generally arranged inside the micro-fluidic chip, and the culture medium is filtered through external filtering equipment, so that the investment of the external equipment is increased.
(II) technical scheme
The utility model discloses a following technical scheme realizes: the utility model provides a micro-fluidic device for cell culture, including film, middle diaphragm and cover plate, the film upside is provided with middle diaphragm, middle diaphragm upside is provided with the cover plate, middle diaphragm one end upside center is provided with the feed liquor hole, middle diaphragm other end upside center is provided with out the liquid hole, middle diaphragm center is embedded to have the cell cavity, cell cavity symmetry both sides are provided with medium filtration membrane.
Further, the cell cavity is close to the inboard integrated into one piece of feed liquor hole one end has the feed liquor pipe, the cell cavity is close to the inboard integrated into one piece of play liquid hole one end has the drain pipe.
By adopting the technical scheme, the liquid inlet pipe and the liquid outlet pipe can convey nutrient liquid required by cell culture into the cell cavity, so that nutrition and a simulated environment are provided for the growth of cells.
Furthermore, the two ends of the upper side of the cover plate are drilled with positioning pressure holes, and the positioning pressure holes corresponding to the liquid inlet holes and the liquid outlet holes on the cover plate are communicated with each other.
Through adopting above-mentioned technical scheme, through location pressure hole, the mould can with the cover plate sticiss in middle diaphragm and on the bottom plate, guarantee the inboard tightness of middle diaphragm guarantees that the cell growth environment is stable, the feed liquor hole with go out the liquid hole with the intercommunication in location pressure hole can guarantee that the transport of nutrient solution is unblocked.
Furthermore, simulation cavities are symmetrically arranged on the inner sides of the cell cavities, and simulation tubes are arranged between the simulation cavities and the liquid inlet tube and between the simulation cavities and the liquid outlet tube.
Through adopting above-mentioned technical scheme, the simulation cavity can simulate different organ internal environment, the simulation pipe can simulate different biological tube tissue environment, and then guarantees cell culture's accuracy nature.
Further, the simulation tube and the cell chamber are of an integrated structure, and the simulation chamber is of a spiral tubular structure.
By adopting the technical scheme, the communication between the simulation chamber and the simulation tube can ensure that the cells in the simulation tube and the cells in the simulation chamber are mutually fused and contacted.
Furthermore, the bottom plate and the cover plate are provided with a sealing rubber pad on one side contacting with the middle membrane, and the sealing rubber pad is glued with the bottom plate and the cover plate.
By adopting the technical scheme, the sealing rubber mat can ensure the contact tightness of the bottom sheet and the cover sheet with the middle diaphragm, thereby ensuring the living environment of cells.
(III) advantageous effects
Compared with the prior art, the utility model, following beneficial effect has:
when solving current micro-fluidic device for cell culture when using, the inside medium filtration membrane that does not generally set up of micro-fluidic chip filters the culture medium through outside filtration equipment, increases the problem of external equipment's input, the utility model discloses a medium filtration membrane that sets up for the micro-fluidic device is inside to carry out the selectivity to the culture medium and filters, need not go on through external equipment, reduces the input of fund.
Drawings
FIG. 1 is an exploded view of a microfluidic device for cell culture according to the present invention;
FIG. 2 is a schematic diagram of a main sectional structure of a cell chamber of the microfluidic device for cell culture according to the present invention;
fig. 3 is a left side view structure diagram of a cell chamber in the microfluidic device for cell culture according to the present invention.
The reference numerals are explained below:
1. a negative film; 2. a cover sheet; 3. an intermediate diaphragm; 4. a liquid inlet hole; 5. a liquid outlet hole; 6. sealing the rubber gasket; 7. a cell chamber; 8. positioning a pressure hole; 9. a liquid inlet pipe; 10. a liquid outlet pipe; 11. a media filtration membrane; 12. a simulation chamber; 13. and simulating a tube.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
As shown in fig. 1-3, the microfluidic device for cell culture in this embodiment includes a bottom plate 1, a middle membrane 3 and a cover plate 2, the middle membrane 3 is disposed on the top side of the bottom plate 1, the cover plate 2 is disposed on the top side of the middle membrane 3, a liquid inlet hole 4 is disposed in the center of the top side of one end of the middle membrane 3, a liquid outlet hole 5 is disposed in the center of the top side of the other end of the middle membrane 3, a cell chamber 7 is embedded in the center of the middle membrane 3, medium filtering membranes 11 are disposed on two symmetrical sides of the cell chamber 7, and the culture medium is selectively filtered inside the microfluidic device through the arranged medium filtering membranes 11 without being processed through an external device, thereby reducing investment.
2 upside both ends of cover plate are bored and are equipped with location pressure hole 8, and the location pressure hole 8 that corresponds with feed liquor hole 4 and play liquid hole 5 on the cover plate 2 link up each other, and through location pressure hole, the mould can sticis cover plate 2 on middle diaphragm 3 and film 1, guarantees the inboard tightness of middle diaphragm 3, guarantees that the cell growth environment is stable, and feed liquor hole 4 and play liquid hole 5 and location pressure hole 8's intercommunication can guarantee that the transport of nutrient solution is unblocked.
The simulation tube 13 and the cell chamber 7 are of an integral structure, the simulation chamber 12 is of a spiral tubular structure, and the communication between the simulation chamber 12 and the simulation tube 13 can enable the cells in the simulation tube 13 and the cells in the simulation chamber 12 to be in fusion contact with each other.
The contact sides of the bottom plate 1 and the cover plate 2 with the middle membrane 3 are provided with a sealing rubber pad 6, the sealing rubber pad 6 is in glue joint with the bottom plate 1 and the cover plate 2, the sealing rubber pad 6 can enable the bottom plate 1 and the cover plate 2 to be in contact tightness with the middle membrane 3, and then the living environment of cells is guaranteed.
The specific implementation process of this embodiment is as follows: when in use, the middle film 3 is firstly placed on the upper side of the bottom plate 1, the cover plate 2 is respectively aligned with the middle film 3 and the bottom plate 1 up and down, then the aligned device is placed into a compaction die, the cover plate 2 can be tightly pressed on the upper sides of the middle membrane 3 and the bottom plate 1 through the positioning pressure holes 8 on the upper side of the cover plate 2, the culture medium conveying pipe is connected with the positioning pressure holes 8 on the upper sides of the liquid inlet hole 4 and the liquid outlet hole 5, the culture medium is conveyed into the liquid inlet pipe 9, after the culture medium is filtered by the medium filtering membrane 11, culture medium is delivered into the cell chamber 7, the simulation tube 13 and the simulation chamber 12 can provide different simulation environments for the growth of cells, and then guarantee the accuracy nature of cell culture, through the medium filtration membrane 11 that sets up for the inside culture medium that carries out the selectivity of micro-fluidic device filters, need not go on through external equipment, reduces the input of fund.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention and are not intended to limit the spirit and scope of the present invention. Without departing from the design concept of the present invention, various modifications and improvements made by the technical solution of the present invention by those skilled in the art should fall into the protection scope of the present invention, and the technical contents claimed by the present invention have been fully recorded in the claims.
Claims (6)
1. A microfluidic device for cell culture, characterized in that: including film (1), middle diaphragm (3) and cover plate (2), film (1) upside is provided with middle diaphragm (3), middle diaphragm (3) upside is provided with cover plate (2), middle diaphragm (3) one end upside center is provided with feed liquor hole (4), middle diaphragm (3) other end upside center is provided with out liquid hole (5), middle diaphragm (3) center is embedded to have cell cavity (7), cell cavity (7) symmetry both sides are provided with medium filtration membrane (11).
2. A microfluidic device for cell culture according to claim 1, wherein: cell cavity (7) are close to feed liquor hole (4) one end inboard integrated into one piece has feed liquor pipe (9), cell cavity (7) are close to go out liquid hole (5) one end inboard integrated into one piece has drain pipe (10).
3. A microfluidic device for cell culture according to claim 1, wherein: positioning pressure holes (8) are drilled in two ends of the upper side of the cover plate (2), and the positioning pressure holes (8) which correspond to the liquid inlet holes (4) and the liquid outlet holes (5) are communicated with each other on the cover plate (2).
4. A microfluidic device for cell culture according to claim 2, wherein: cell cavity (7) inboard symmetry is provided with simulation cavity (12), simulation cavity (12) with feed liquor pipe (9) and be provided with simulation pipe (13) between drain pipe (10).
5. A microfluidic device for cell culture according to claim 4, wherein: the simulation tube (13) and the cell chamber (7) are of an integral structure, and the simulation chamber (12) is of a spiral tubular structure.
6. A microfluidic device for cell culture according to claim 1, wherein: the negative film (1) and the cover plate (2) with the middle diaphragm (3) contact side is provided with a sealing rubber mat (6), and the sealing rubber mat (6) is glued with the negative film (1) and the cover plate (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022470081.5U CN214142391U (en) | 2020-10-30 | 2020-10-30 | Micro-fluidic device for cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022470081.5U CN214142391U (en) | 2020-10-30 | 2020-10-30 | Micro-fluidic device for cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214142391U true CN214142391U (en) | 2021-09-07 |
Family
ID=77561729
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022470081.5U Active CN214142391U (en) | 2020-10-30 | 2020-10-30 | Micro-fluidic device for cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214142391U (en) |
-
2020
- 2020-10-30 CN CN202022470081.5U patent/CN214142391U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10481150B2 (en) | Microfluidic device for cell-based assays | |
| Hosic et al. | Rapid prototyping of multilayer microphysiological systems | |
| CN106085846A (en) | The adapter of the pneumatic means in microfluid system | |
| WO2013094370A1 (en) | Film-shaped tissue storage transport container and storage transport method | |
| WO2012033439A1 (en) | Microfluidic capsule | |
| CN105385597B (en) | A kind of cell culture bags and its application | |
| CN214142391U (en) | Micro-fluidic device for cell culture | |
| CN104083819A (en) | Precise liquid medicine filter for medical infusion set | |
| US20130236972A1 (en) | Liver Sinusoid Model | |
| CN114231414A (en) | Bone tissue bionic chip constructed based on microfluidic technology and application thereof | |
| CN117721019A (en) | Vascularized organ chip and application thereof | |
| CN107955790B (en) | Intestine-kidney system for simulating in-vivo absorption process of medicine based on micro-fluidic chip | |
| CN212688016U (en) | Biomembrane dynamic culture system under simulation in vivo flow environment | |
| CN202699722U (en) | Disposable infusion device with liquid-stopping empty-dropping device | |
| CN112375681B (en) | Organ chip and application thereof | |
| CN115161193A (en) | Optimization method for preparing intestinal organ chip for exploring mercury ions | |
| CN212128201U (en) | Cell co-culture system for simulating hepatic sinus | |
| CN204017041U (en) | A kind of medical Transfusion device precision liquid medicine filter | |
| JP6236522B2 (en) | Cell culture device, cell culture system and cell culture method | |
| JPWO2020017067A1 (en) | Attachment for perfusion culture | |
| CN208464401U (en) | A kind of bladder stoma bag of waterproof ventilated membrane | |
| CN219363622U (en) | Microorganism detection filter | |
| CN214529033U (en) | A collection fungus culture apparatus for public health prevention branch of academic or vocational study | |
| CN204079953U (en) | A kind of plastic uptake bag packaging structure of rare cell separometer | |
| CN213935366U (en) | A simulation trainer for breast palpation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A microfluidic device for cell culture Effective date of registration: 20220704 Granted publication date: 20210907 Pledgee: Kaifeng branch of Zhongyuan Bank Co.,Ltd. Pledgor: HENAN PEVI TECHNOLOGY CO.,LTD. Registration number: Y2022980009730 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20230628 Granted publication date: 20210907 Pledgee: Kaifeng branch of Zhongyuan Bank Co.,Ltd. Pledgor: HENAN PEVI TECHNOLOGY CO.,LTD. Registration number: Y2022980009730 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A Microfluidics device for cell culture Effective date of registration: 20230629 Granted publication date: 20210907 Pledgee: Kaifeng branch of Zhongyuan Bank Co.,Ltd. Pledgor: HENAN PEVI TECHNOLOGY CO.,LTD. Registration number: Y2023980046804 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Granted publication date: 20210907 Pledgee: Kaifeng branch of Zhongyuan Bank Co.,Ltd. Pledgor: HENAN PEVI TECHNOLOGY CO.,LTD. Registration number: Y2023980046804 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A microfluidic device for cell culture Granted publication date: 20210907 Pledgee: Kaifeng branch of Zhongyuan Bank Co.,Ltd. Pledgor: HENAN PEVI TECHNOLOGY CO.,LTD. Registration number: Y2024980024667 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |