CN212700663U - Macroporous resin chromatography device - Google Patents
Macroporous resin chromatography device Download PDFInfo
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- CN212700663U CN212700663U CN202021108170.9U CN202021108170U CN212700663U CN 212700663 U CN212700663 U CN 212700663U CN 202021108170 U CN202021108170 U CN 202021108170U CN 212700663 U CN212700663 U CN 212700663U
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Abstract
The utility model provides a macroporous resin chromatography device, which comprises a chromatography column body, wherein the chromatography column body comprises a first column body and a second column body which are arranged from top to bottom, the first column body is detachably connected with the second column body, macroporous resin is filled in the chromatography column body, a first heating sleeve is sleeved on the outer side of the first column body, and a second heating sleeve is sleeved on the outer side of the second column body; the top of first cylinder is provided with the closing cap, be provided with the inlet on the closing cap respectively and be used for volatile component exhaust volatile substance export, the inlet is connected with the feed liquor pipe, the feed liquor union coupling has the peristaltic pump for realize that the feed liquid is in be interrupted the feeding on the chromatography cylinder, volatile substance exit linkage has recovery unit, the bottom of second cylinder is connected with the drain pipe. The utility model discloses a macroporous resin chromatography device can dismantle two cylinders of connection through setting up, has simplified the operation, and easy the washing is dismantled easily.
Description
Technical Field
The utility model relates to a traditional chinese medicine draws the purification field, particularly, relates to a macroporous resin chromatography device for traditional chinese medicine active ingredient separation and purification.
Background
The chemical components contained in the traditional Chinese medicine are very complex, and not only contain various effective components, but also have ineffective components and also contain toxic components. Extracting the effective components of the effective monomers, and further separating and purifying the effective components to obtain the effective monomers is an important content in the research field of traditional Chinese medicines. The extraction of the traditional Chinese medicine is to extract the effective components in the traditional Chinese medicine to the maximum extent by utilizing certain technologies, so that the internal quality and the clinical treatment effect of the traditional Chinese medicine preparation are improved, and the effect of the traditional Chinese medicine is exerted to the maximum extent.
The macroporous resin chromatographic column is a common device used in the extraction process of the active ingredients of the traditional Chinese medicine, the active ingredients of the traditional Chinese medicine are adsorbed on the macroporous resin in the chromatographic column, the result of intermolecular force mainly comprising Van der Waals force and hydrogen bond formed between the active ingredients and the macroporous resin is mainly utilized, and the main operation method comprises the following steps: the chromatographic column is flowed through by sample loading liquid containing active ingredients at a certain flow velocity, so that the active ingredients can be adsorbed, then the eluent is eluted and collected, but after the macroporous resin is repeatedly used, the cleaning is needed after the clogging is caused, most of macroporous resin chromatographic columns on the market at present are of an integral structure, the disassembly is inconvenient, great difficulty is brought to the cleaning and the subsequent purification operation, and when the macroporous resin needs to be replaced, because many solvents adsorbed on the macroporous resin are organic matters, if the organic matters are directly discarded, the environmental pollution is serious, and the human health is influenced.
In addition, in the use process of the macroporous resin chromatographic column in the prior art, the sample loading liquid is generally in a flowing state with a constant speed, and the sample loading liquid is separated from the chromatographic column without effectively staying on the macroporous resin, so that the purification and separation are incomplete, and the purification and extraction effects of effective components are influenced.
In view of this, the present invention is especially provided.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a macroporous resin chromatography device, design through this chromatography device, can dismantle two chromatography cylinders of connection through setting up on the one hand, the operation has been simplified, easy washing, easy dismantlement, on the other hand makes the active ingredient in the feed liquid can be drawed by more efficient through the mode of being interrupted the feeding, the purification effect is also better, and can also open the hot water jacket of installing before macroporous resin needs to be changed, evaporate the recovery to the last adsorbed volatile organic matter of macroporous resin, prevent the follow-up destruction to the environment of abandoning of macroporous resin.
In order to realize the above purpose of the utility model, the following technical scheme is adopted:
the utility model provides a macroporous resin chromatography device, which comprises a chromatography column body, wherein the chromatography column body comprises a first column body and a second column body which are arranged from top to bottom, the first column body is detachably connected with the second column body, macroporous resin is filled in the chromatography column body, a first heating sleeve is sleeved on the outer side of the first column body, and a second heating sleeve is sleeved on the outer side of the second column body; the top of the first heating sleeve is provided with a first hot water inlet, the bottom of the first heating sleeve is provided with a first hot water outlet, the top of the second heating sleeve is provided with a second hot water inlet, and the bottom of the second heating sleeve is provided with a second hot water outlet;
the top of first cylinder is provided with the closing cap, be provided with the inlet on the closing cap respectively and be used for volatile substance of volatile component exhaust export, the inlet is connected with the feed liquor pipe, the feed liquor union coupling has the peristaltic pump for realize that the feed liquid is in be interrupted the feeding in the chromatography main part, volatile substance exit linkage has recovery unit, the bottom of second cylinder is connected with the drain pipe.
The utility model discloses a mutual detachable chromatography cylinder that sets up has simplified the operation, washs more easily, has avoided overall structure to bring the inconvenient problem of dismantlement and has taken place.
Through the peristaltic pump who sets up, can make the appearance liquid of going up advance kind with discontinuous form, increased the dwell time of feed liquid in post inside, effectively promoted separation and purification effect.
Through the hot water jacket structure who sets up, before macroporous resin need be changed, evaporate the recovery unit outside the volatile organic compounds of absorption on the macroporous resin and carry out the recovery, prevent the follow-up destruction to the environment of abandoning of macroporous resin.
Drawings
Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the invention. Also, like reference numerals are used to refer to like parts throughout the drawings. In the drawings:
fig. 1 is a schematic structural diagram of a macroporous resin chromatography device according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of the structure of a closure cap in the macroporous resin chromatography device of FIG. 1;
fig. 3 is a schematic structural diagram of macroporous resin chromatographic columns connected in parallel according to the embodiment of the present invention.
Description of the drawings:
10-a chromatography column; 101-a first cylinder;
102-a second cylinder; 103-sealing cover;
1031-liquid inlet; 1032-volatile outlet;
1033-pressure gauge; 1034-a pressure relief valve;
1035-recovery unit; 20-a liquid inlet pipe;
30-a peristaltic pump; 40-grooves;
50-a bump; 60-buckling;
70-a liquid outlet pipe; 80-a sample collector;
90-a flow controller; 100-a liquid inlet control valve;
110-a first heating jacket; 1101-a first hot water inlet;
1102-a first hot water outlet; 120-a second heating jacket;
1201-a second hot water inlet; 1202-second hot water outlet.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings and detailed description, but those skilled in the art will understand that the following described embodiments are some, not all, of the embodiments of the present invention, and are only used for illustrating the present invention, and should not be construed as limiting the scope of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The utility model provides a macroporous resin chromatography device, which comprises a chromatography column body, wherein the chromatography column body comprises a first column body and a second column body which are arranged from top to bottom, the first column body is detachably connected with the second column body, macroporous resin is filled in the chromatography column body, a first heating sleeve is sleeved on the outer side of the first column body, and a second heating sleeve is sleeved on the outer side of the second column body; the top of the first heating sleeve is provided with a first hot water inlet, the bottom of the first heating sleeve is provided with a first hot water outlet, the top of the second heating sleeve is provided with a second hot water inlet, and the bottom of the second heating sleeve is provided with a second hot water outlet;
the top of first cylinder is provided with the closing cap, be provided with the inlet on the closing cap respectively and be used for volatile component exhaust volatile substance export, the inlet is connected with the feed liquor pipe, the feed liquor union coupling has the peristaltic pump for realize that the feed liquid is in be interrupted the feeding on the chromatography cylinder, volatile substance exit linkage has recovery unit, the bottom of second cylinder is connected with the drain pipe.
Macroporous resin chromatographic column belongs to the separation and purification equipment commonly used among the prior art, but it is because overall structure can't dismantle, has brought some degrees of difficulty for changing, rinsing filler wherein, the utility model discloses a solve above-mentioned technical problem and provide a split type macroporous resin chromatographic column specially, upper and lower two-layer cylinder can be dismantled mutually, has made things convenient for follow-up dismantlement operation like this, and is also more convenient during the use, can realize zonulae occludens after the butt joint.
In addition, in order to improve the purification effect of the sample, the liquid inlet pipe is also connected with a peristaltic pump, the peristaltic pump realizes the mode of discontinuous feeding, and the retention time of the sample in the column is prolonged, so that the adsorption and purification are more sufficient, and the purification effect is more excellent.
The utility model discloses still all set up the hot water jacket in the outside of first cylinder and second cylinder, when the macroporous resin filler of inside dress need be changed, open the hot water jacket earlier and carry out evaporation recovery to the volatile component that adsorbs on the macroporous resin, when preventing that macroporous resin from changing again and abandoning, not friendly to the environment, also there is the harm of certain degree to operating personnel's health.
Further, first cylinder bottom is provided with the lug, second cylinder top is provided with the recess, the lug with the recess mutually supports in order to realize dismantling the connection. In addition, other detachable connection modes are also within the protection scope of the present invention.
Furthermore, a buckle matched with each other is arranged between the first cylinder and the second cylinder. In order to guarantee that the first cylinder and the second cylinder are fixed after being butted with each other, the buckle matched with each other is arranged between the first cylinder and the second cylinder, the specific structure of the buckle can be formed by uniformly distributing a plurality of clamping blocks in the circumferential direction of the first cylinder and the second cylinder, and the clamping connection can be carried out more tightly after the butt joint.
Furthermore, the mutually matched parts of the convex block and the groove are provided with buffer pads. The buffer pad has excellent static buffer performance and can also play a certain sealing effect.
Furthermore, the drain pipe is connected with a sample collector, and a flow controller for controlling the flow rate is arranged on the drain pipe. The sample flow of the liquid outlet pipe can be controlled and adjusted, the flow can be set according to the valve, and the use is convenient.
Further, in order to accurately observe the flow, a display screen for displaying the flow is arranged on the flow controller.
Furthermore, the chromatography cylinder is a plurality of, every connect in parallel each other between the chromatography cylinder, every the feed liquor pipe at chromatography cylinder top converges the back with the peristaltic pump links to each other, every the drain pipe of chromatography cylinder bottom converges the back with the sample collector links to each other. In order to improve the purification effect, a plurality of macroporous resin chromatographic columns can be connected in parallel for operation, and the purification operation is carried out simultaneously.
Furthermore, each liquid inlet pipe is provided with a liquid inlet control valve for selecting flowing through the pipeline, the design of the liquid inlet control valve can be used for selectively controlling each flowing through pipeline, and the flowing through pipeline of the liquid is controlled by opening and closing the liquid inlet control valve.
Furthermore, a pressure gauge and a pressure release valve are arranged on the sealing cover to prevent the over-high pressure in the chromatographic column from causing potential safety hazards.
Further, the feed liquor pipe with the material of drain pipe is soft materials, further can be plastics material or rubber material etc. for example the PVC material can.
In order to clarify the technical solution of the present invention, the following description is made in the form of specific embodiments.
Examples
As shown in fig. 1-3, the embodiment of the utility model provides a macroporous resin chromatography device, including the chromatography cylinder, the chromatography cylinder comprises first cylinder 101 and second cylinder 102, first cylinder 101 sets up from top to bottom with second cylinder 102, and be dismantled between first cylinder 101 and the second cylinder 102 and be connected, it has macroporous resin to pack in chromatography cylinder 10, macroporous resin has good adsorptivity as adsorption material, can select the type of the macroporous resin of packing according to the specific nature of the active ingredient of chinese-medicinal material during actual operation, it is more showing to guarantee to draw the extraction rate of active ingredient.
A first heating sleeve is sleeved on the outer side of the first cylinder 101, and a second heating sleeve is sleeved on the outer side of the second cylinder; the top of the first heating jacket 110 is provided with a first hot water inlet 1101, the bottom of the first heating jacket is provided with a first hot water outlet 1102, the top of the second heating jacket 120 is provided with a second hot water inlet 1201, the bottom of the second heating jacket is provided with a second hot water outlet 1202, and the arrangement of the inlet and the outlet realizes the circulation of hot water in the jacket.
The top of the first column 101 is provided with a sealing cover 103, the sealing cover 103 is respectively provided with a liquid inlet 1031, a volatile substance outlet 1032, a pressure gauge 1033 and a pressure release valve 1034, the liquid inlet is connected with a liquid inlet pipe 20, and the liquid inlet pipe 20 is connected with a peristaltic pump 30 to realize the intermittent feeding of the sample. The volatile outlet 1032 is connected to a recovery unit 1035 to recover the volatile components for further processing.
The bottom of the second column 102 is connected with a liquid outlet pipe 70, the liquid outlet pipe 70 is connected with a sample collector 80, and the sample collector 80 is used for collecting sample liquid containing effective components.
The outlet pipe 70 of this embodiment is further provided with a flow controller 90 for controlling the flow rate, and in order to monitor the flow more conveniently, the flow controller 90 is provided with a display screen for displaying the flow.
In the macroporous resin chromatography device in this embodiment, what first cylinder 101 and second cylinder 102 took is the mode of can dismantling the connection, is specifically provided with lug 50 in the bottom of first cylinder 101, and second cylinder 102 top is provided with recess 40, first cylinder 101 with be provided with mutually supporting buckle 60 between the second cylinder 102, lug 50 and recess 40 dock each other the back, pass through buckle 60 seal joint with first cylinder 101 and second cylinder 102 through the rotation to guarantee good fixed effect, moreover the leakproofness is relatively excellent.
In order to improve the sealing performance between the first cylinder 101 and the second cylinder 102 and buffer the stress, a buffer pad is further disposed at the mutual matching position of the projection 50 and the groove 40.
In the macroporous resin chromatography device of the embodiment, a plurality of chromatography columns 10 are provided, each chromatography column 10 is connected in parallel, the liquid inlet pipe 20 at the top of each chromatography column 10 is converged and then connected with the peristaltic pump 30, and the liquid outlet pipe 70 at the bottom of each chromatography column 10 is converged and then connected with the sample collector 80. To facilitate the selection of the flow path, a liquid inlet control valve 100 is provided on each liquid inlet pipe 20. The liquid inlet control valve 100 may be a ball valve, a butterfly valve, or a solenoid valve.
The material of the liquid inlet pipe 20 and the liquid outlet pipe 70 is preferably soft material, which is flexible when operated manually. Of course, hard materials may be used to facilitate the installation of other components.
During specific operation, the sample is firstly loaded through the liquid inlet pipe at a certain flow rate, and then the eluent is adopted for elution so as to ensure that the sample liquid with the effective components of the traditional Chinese medicine is obtained, wherein the specific operation mode is similar to the operation steps of the macroporous resin chromatographic column in the prior art.
In a word, the utility model discloses a mutual detachable chromatography cylinder that sets up has simplified the operation, washs more easily, has avoided overall structure to bring the inconvenient problem of dismantlement and has taken place.
Through the peristaltic pump who sets up, can make the appearance liquid of going up advance kind with the mode of interrupting, increased the dwell time of feed liquid in post inside, effectively promoted separation and purification effect.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention.
Claims (10)
1. The macroporous resin chromatography device is characterized by comprising a chromatography column body, wherein the chromatography column body comprises a first column body and a second column body which are arranged from top to bottom, the first column body is detachably connected with the second column body, macroporous resin is filled in the chromatography column body, a first heating sleeve is sleeved on the outer side of the first column body, and a second heating sleeve is sleeved on the outer side of the second column body; the top of the first heating sleeve is provided with a first hot water inlet, the bottom of the first heating sleeve is provided with a first hot water outlet, the top of the second heating sleeve is provided with a second hot water inlet, and the bottom of the second heating sleeve is provided with a second hot water outlet;
the top of first cylinder is provided with the closing cap, be provided with the inlet on the closing cap respectively and be used for volatile component exhaust volatile substance export, the inlet is connected with the feed liquor pipe, the feed liquor union coupling has the peristaltic pump for realize that the feed liquid is in be interrupted the feeding on the chromatography cylinder, volatile substance exit linkage has recovery unit, the bottom of second cylinder is connected with the drain pipe.
2. The macroporous resin chromatography device of claim 1, wherein a projection is disposed at the bottom of the first column and a groove is disposed at the top of the second column, and the projection and the groove cooperate to achieve the detachable connection.
3. The macroporous resin chromatography device of claim 2, wherein a snap fit is provided between the first and second columns.
4. The macroporous resin chromatography device of claim 2, wherein the interfitting portions of the projection and the recess are provided with a cushion.
5. The macroporous resin chromatography device of claim 1, wherein the effluent tube is connected to a sample collector and a flow controller is disposed on the effluent tube for controlling the flow rate of the effluent.
6. The macroporous resin chromatography device of claim 5, wherein a display screen is provided on the flow controller to display the flow rate.
7. The macroporous resin chromatography device of claim 6, wherein the chromatography column is multiple, each chromatography column is connected in parallel, a liquid inlet pipe at the top of each chromatography column is connected with the peristaltic pump after being converged, and a liquid outlet pipe at the bottom of each chromatography column is connected with the sample collector after being converged.
8. The macroporous resin chromatography device of claim 7, wherein each of the inlet tubes is provided with an inlet control valve for selectively passing through the conduit.
9. The macroporous resin chromatography device of claim 1, wherein the closure cap is provided with a pressure gauge and a pressure relief valve.
10. The macroporous resin chromatography device of any one of claims 1-9, wherein the liquid inlet tube and the liquid outlet tube are both made of soft materials.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021108170.9U CN212700663U (en) | 2020-06-16 | 2020-06-16 | Macroporous resin chromatography device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021108170.9U CN212700663U (en) | 2020-06-16 | 2020-06-16 | Macroporous resin chromatography device |
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| Publication Number | Publication Date |
|---|---|
| CN212700663U true CN212700663U (en) | 2021-03-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202021108170.9U Active CN212700663U (en) | 2020-06-16 | 2020-06-16 | Macroporous resin chromatography device |
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| CN (1) | CN212700663U (en) |
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