CN212688063U - Microorganism culture bottle - Google Patents
Microorganism culture bottle Download PDFInfo
- Publication number
- CN212688063U CN212688063U CN202021448535.2U CN202021448535U CN212688063U CN 212688063 U CN212688063 U CN 212688063U CN 202021448535 U CN202021448535 U CN 202021448535U CN 212688063 U CN212688063 U CN 212688063U
- Authority
- CN
- China
- Prior art keywords
- bottle
- microorganism
- filter membrane
- sealing ring
- pipe connector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 244000005700 microbiome Species 0.000 title abstract description 38
- 238000007789 sealing Methods 0.000 claims abstract description 22
- 239000012528 membrane Substances 0.000 claims abstract description 21
- 230000000813 microbial effect Effects 0.000 claims abstract description 13
- 238000009629 microbiological culture Methods 0.000 claims 1
- 238000001914 filtration Methods 0.000 abstract description 14
- 238000000034 method Methods 0.000 abstract description 6
- 239000001963 growth medium Substances 0.000 abstract description 5
- 238000009630 liquid culture Methods 0.000 abstract description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 229910001873 dinitrogen Inorganic materials 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a microorganism culture bottle, which comprises a bottle body, a bottle bottom, a microorganism filter membrane, a supporting plate with holes, a first sealing ring and a second sealing ring, wherein the lower end of the bottle body is connected with the upper end of the bottle bottom through threads, an annular flange is arranged on the inner wall of the lower end of the bottle body, and the first sealing ring, the microorganism filter membrane, the supporting plate and the second sealing ring are sequentially arranged between the annular flange and the upper end surface of the bottle bottom from top to bottom; the side wall of the bottle bottom is provided with a pipe connector and a sealing cover arranged on the pipe connector. The utility model discloses microorganism blake bottle, its function that possesses the filtration microorganism simultaneously and cultivates the microorganism for after filtering the enrichment microorganism through the filter membrane, can directly carry out the microbial cultivation to adding liquid culture medium in the blake bottle, no longer need shift the filter membrane, simplified microorganism cultivation operation process greatly, improved microorganism cultivation work efficiency, and can avoid shifting the pollution risk that the filter membrane exists.
Description
Technical Field
The utility model relates to a microbial cultivation device, in particular to a microbial cultivation bottle.
Background
In the prior art, during the process of culturing microorganisms, a membrane filtration device is usually used to filter the microorganisms in the liquid to be tested, and then the filter membrane is transferred to a plate culture medium or a culture flask for culturing. The transfer filter membrane needs to be disassembled from the filter device, the operation is complex, and the transfer filter membrane is easily polluted by microorganisms in the air in the process.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model aims at providing a microorganism blake bottle to solve among the prior art microorganism filtration and cultivate by the device completion of difference, shift the technical problem such as filter membrane troublesome poeration, easily contaminated.
The utility model discloses a microorganism culture bottle, including body, bottle bottom, microbial filtration membrane, foraminiferous backup pad, first sealing washer and second sealing washer, the lower extreme of body passes through threaded connection with the upper end of bottle bottom, is provided with annular flange on the lower extreme inner wall of body, first sealing washer, microbial filtration membrane, backup pad and second sealing washer from last to setting gradually between annular flange and the bottle bottom up end down;
the side wall of the bottle bottom is provided with a pipe connector and a sealing cover arranged on the pipe connector.
Further, the upper end oral area of body is provided with the bottle lid, be provided with one-way admission valve and one-way discharge valve on the bottle lid.
The utility model has the advantages that:
1. the utility model discloses microorganism blake bottle, its function that possesses the filtration microorganism simultaneously and cultivates the microorganism for after filtering the enrichment microorganism through the filter membrane, can directly carry out the microorganism culture to adding liquid culture medium in the blake bottle, no longer need shift the filter membrane, simplified microorganism culture operation process greatly, improved microorganism culture work efficiency, and avoided shifting the pollution risk that the filter membrane exists.
2. The utility model discloses microorganism blake bottle, the bottle lid that sets up on its body has one-way admission valve and one-way discharge valve for when cultivateing anaerobic microorganisms, can fill into carbon dioxide or nitrogen gas through one-way admission valve in to the blake bottle, thereby build the anaerobic environment that is suitable for anaerobic microorganisms to grow.
Drawings
FIG. 1 is a schematic view of a first structure of a microorganism culture flask;
FIG. 2 is a schematic view of a second structure of the microorganism culture flask;
fig. 3 is an enlarged schematic view of the portion P in fig. 1.
Detailed Description
The invention is further described with reference to the following figures and examples.
As shown in the figure, this embodiment microorganism blake bottle, including body 1, bottle end 2, microbial filtration membrane 3, foraminiferous backup pad 4, first sealing washer 5 and second sealing washer 6, threaded connection is passed through with the upper end at the bottom of the bottle to the lower extreme of body, is provided with ring flange 7 on the lower extreme inner wall of body, first sealing washer, microbial filtration membrane, backup pad and second sealing washer from last to setting gradually between ring flange and the bottle end up end down.
The side wall of the bottle bottom is provided with a pipe connector 8 and a sealing cover 9 arranged on the pipe connector.
When in use, the sealing cover 9 is pulled out, the pipe connector at the bottom of the bottle is connected with the suction pump through a hose, the liquid to be filtered is led into the microorganism culture bottle, and the suction pump is started to finish the filtration; the first sealing washer and the second sealing washer can guarantee that filtrating can not reveal from the joint portion of bottle end and body in the filtering process. After the filtration is finished, the sealing cover is covered on the pipe connector again, and then the culture medium is introduced into the microorganism culture bottle, so that the microorganisms enriched on the filter membrane can be cultured.
The microbial cultivation bottle in this embodiment, it possesses the function of filtering microorganism and cultivateing the microorganism simultaneously for after filtering the enrichment microorganism through the filter membrane, can directly add liquid culture medium to the blake bottle and carry out the microbial cultivation, no longer need shift the filter membrane, simplified microbial cultivation operation process greatly, improved microbial cultivation work efficiency, and avoided shifting the pollution risk that the filter membrane exists.
As a modification of the above embodiment, a bottle cap 10 is provided at the upper end opening of the bottle body, and a one-way intake valve 11 and a one-way exhaust valve 12 are provided on the bottle cap. This improvement makes when cultivateing anaerobic microorganisms, can fill into carbon dioxide or nitrogen gas through one-way admission valve to the blake bottle to build the anaerobic environment who is suitable for anaerobic microorganisms to grow.
Finally, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the present invention can be modified or replaced by other means without departing from the spirit and scope of the present invention, which should be construed as limited only by the appended claims.
Claims (2)
1. A microbial cultivation bottle, which is characterized in that: the bottle comprises a bottle body, a bottle bottom, a microbial filter membrane, a supporting plate with holes, a first sealing ring and a second sealing ring, wherein the lower end of the bottle body is connected with the upper end of the bottle bottom through threads;
the side wall of the bottle bottom is provided with a pipe connector and a sealing cover arranged on the pipe connector.
2. The microbial culture flask of claim 1, wherein: the bottle is characterized in that a bottle cap is arranged at the upper end opening of the bottle body, and a one-way air inlet valve and a one-way air outlet valve are arranged on the bottle cap.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021448535.2U CN212688063U (en) | 2020-07-21 | 2020-07-21 | Microorganism culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021448535.2U CN212688063U (en) | 2020-07-21 | 2020-07-21 | Microorganism culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212688063U true CN212688063U (en) | 2021-03-12 |
Family
ID=74898508
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021448535.2U Expired - Fee Related CN212688063U (en) | 2020-07-21 | 2020-07-21 | Microorganism culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212688063U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115530072A (en) * | 2022-09-02 | 2022-12-30 | 广西壮族自治区林业科学研究院 | Culture bottle for solving browning of African daisy culture material |
| CN116075586A (en) * | 2020-07-08 | 2023-05-05 | 快速微型生物系统公司 | Filtration assemblies, cartridges, systems and methods for filtration and cell growth |
-
2020
- 2020-07-21 CN CN202021448535.2U patent/CN212688063U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116075586A (en) * | 2020-07-08 | 2023-05-05 | 快速微型生物系统公司 | Filtration assemblies, cartridges, systems and methods for filtration and cell growth |
| CN115530072A (en) * | 2022-09-02 | 2022-12-30 | 广西壮族自治区林业科学研究院 | Culture bottle for solving browning of African daisy culture material |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210312 |