CN212655801U

CN212655801U - Ovum maturity culture dish

Info

Publication number: CN212655801U
Application number: CN202021992619.2U
Authority: CN
Inventors: 贾启鹏; 刘湘娟; 王伟
Original assignee: Inlems Life Technology Shenzhen Co ltd
Current assignee: Inlems Life Technology Shenzhen Co ltd
Priority date: 2020-09-11
Filing date: 2020-09-11
Publication date: 2021-03-05
Anticipated expiration: 2030-09-11

Abstract

An ovum maturation culture dish comprises a culture dish body, a culture dish cover and an ovum culture tank, wherein the upper surface of the culture dish body is provided with a convex dish ring, the culture dish cover consists of a dish cover part and a dish sleeve part, and the ovum culture tank is arranged on the culture dish body and is of a truncated cone-shaped tank body structure; the dish cover portion is coaxially sleeved with the protruding dish ring, the dish cover portion and the protruding dish ring are together formed with a culture chamber on the upper surface side of the culture dish body, and a gas exchange channel communicated with the culture chamber is arranged on the dish cover portion. Because the egg culture tank is in a round platform-shaped tank body structure, the eggs can be automatically gathered, and information and substance exchange among the eggs can be facilitated; meanwhile, the culture chamber exchanges gas with the external environment through the gas exchange channel, so that the time of gas balance inside and outside the culture dish can be shortened, and the ovum is maintained to be always in a stable PH environment; therefore, conditions can be created for improving the maturation rate of the ovum, and the in-vitro maturation culture effect of the ovum is improved.

Description

Ovum maturity culture dish

Technical Field

The utility model belongs to the technical field of biomedical device technique and specifically relates to an ovum maturity culture dish.

Background

In recent years, with the increasing year by year of the number of patients with infertility, people who accept assisted reproduction and assisted pregnancy are increased, and in the process, the in vitro maturation technology of the ovum obtains better pregnancy outcome for patients who are not suitable to use higher dose of excretion promoting drugs (such as PCOS patients and cancer patients) and patients with poor maturation of the ovum (such as patients with low ovarian function), and simultaneously, provides an opportunity for the immature female cancer patients to breed offspring with self genetic characteristics for preserving fertility; therefore, in vitro maturation culture of ova is considered to be an effective means for enriching assisted reproductive technologies. At present, the effect of in-vitro culture and maturation of immature ova is poor due to the limitations of the existing conditions and technologies, especially due to the structural structure of the culture dish.

SUMMERY OF THE UTILITY MODEL

The utility model discloses the main technical problem who solves provides an ovum maturity culture dish to promote the effect that the external maturity of ovum was cultivateed.

In one embodiment, an egg maturation culture dish is provided, comprising:

the culture dish comprises a culture dish body, wherein a convex dish ring is arranged on the edge of the outline of the upper surface of the culture dish body, and the convex dish ring protrudes out of the upper surface of the culture dish body;

the culture dish cover comprises a dish cover part and a dish sleeve part, the dish sleeve part is formed on the contour edge of the lower surface of the dish cover part, the dish sleeve part is coaxially sleeved with a convex dish ring, a culture chamber is formed on the upper surface side of the culture dish body by the dish cover part and the convex dish ring together, and a gas exchange channel is arranged on the dish sleeve part and is used for communicating the culture chamber with the outside;

and

the egg culture tank is arranged on the upper surface of the culture dish body and is positioned in the culture chamber, and the egg culture tank is of a circular truncated cone-shaped groove body structure with the upper bottom radius larger than the lower bottom radius.

In one embodiment, the method further comprises:

the ovum cleaning tank is arranged on the upper surface of the culture dish body and is positioned in the culture cavity, and the ovum cleaning tank is used for containing an ovum cleaning solution;

and

the egg transfer groove is formed in the upper surface of the culture dish body and is positioned in the culture chamber, and the egg transfer groove is of a circular truncated cone-shaped groove body structure with the upper bottom radius larger than the lower bottom radius;

the culture dish is characterized in that a partition plate is arranged on the upper surface of the culture dish body and used for dividing a surface area enclosed by the convex dish ring into a first part and a second part, the ovum culture tank is located in the first part, and the ovum cleaning tank and the ovum transfer tank are located in the second part.

In one embodiment, the ovum cleaning tank comprises a first cleaning tank and a second cleaning tank, and the tank body depth of the first cleaning tank is smaller than that of the second cleaning tank by taking the upper surface of the culture dish body as a reference surface.

In one embodiment, a first attribute marking position is arranged on the upper surface of the dish cover, a second attribute marking position is arranged on the circumferential wall of the culture dish body, and the first attribute marking position and the second attribute marking position correspond to each other one to one and are used for marking information of an ovum owner.

In one embodiment, a positioning bevel is formed on the outer peripheral surface of the convex dish ring, the positioning bevel is a plane distributed along the axial direction of the convex dish ring, and the dish sleeve part is matched with the convex dish ring;

the gas exchange channel comprises a circulation notch and a clearance flow channel, the circulation notch is arranged on the lower end face of the dish sleeve part and located on two sides of the circumferential direction of the positioning bevel, and the clearance flow channel is formed between the outer peripheral wall of the protruding dish ring and the inner peripheral wall of the dish sleeve part.

In one embodiment, the vessel sleeve part is further provided with a cover lifting protrusion, and the cover lifting protrusion is located on the peripheral wall of the vessel sleeve part and is distributed opposite to the positioning bevel.

In one embodiment, the circulation notch is a U-like or semicircular structure which is opened on the lower end surface of the dish sleeve portion and distributed through the inside and the outside of the dish sleeve portion, and at least two continuously distributed circulation notches are arranged on each side of the circumferential direction of the positioning bevel surface.

The ovum maturation culture dish according to the embodiment comprises a culture dish body, a culture dish cover and an ovum culture groove, wherein the upper surface of the culture dish body is provided with a convex dish ring, the culture dish cover consists of a dish cover part and a dish sleeve part, and the ovum culture groove is arranged on the culture dish body and is of a circular truncated cone-shaped groove body structure; the dish cover portion is coaxially sleeved with the protruding dish ring, the dish cover portion and the protruding dish ring are together formed with a culture chamber on the upper surface side of the culture dish body, and a gas exchange channel communicated with the culture chamber is arranged on the dish cover portion. Because the egg culture tank is in a round platform-shaped tank body structure, the eggs can be automatically gathered, and information and substance exchange among the eggs can be facilitated; meanwhile, the culture chamber exchanges gas with the external environment through the gas exchange channel, so that the time of gas balance inside and outside the culture dish can be shortened, and the ovum is maintained to be always in a stable PH environment; therefore, conditions can be created for improving the maturation rate of the ovum, and the in-vitro maturation culture effect of the ovum is improved.

Drawings

FIG. 1 is a schematic view showing the structural assembly of an ovum maturation culture dish according to an embodiment;

FIG. 2 is a schematic structural exploded view of an egg maturation culture dish according to an embodiment;

FIG. 3 is a schematic structural exploded view of an egg maturation culture dish according to an embodiment;

in the figure:

10. a culture dish body; 11. a raised dish ring; 12. a separator plate; 13. a second attribute flag bit; 14. positioning the sliced noodles;

20. a culture dish cover; 21. a dish cover portion; 22. a dish cover portion; 23. a first attribute flag bit; 24. a flow-through gap; 25. a cover opening bulge;

30. an ovum culture tank; 40. an ovum cleaning tank; 41. a first cleaning tank; 42. a second cleaning tank; 50. an ovum transferring groove.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

Referring to fig. 1, 2 and 3, an embodiment provides an ovum maturation culture dish, which comprises a culture dish body 10, a culture dish cover 20 and an ovum culture tank 30; wherein:

a convex dish ring 11 is arranged on the edge of the outline of the upper surface of the culture dish body 10, and the convex dish ring 11 is distributed on the upper surface of the culture dish body 10 in a protruding manner; the culture dish cover 20 comprises a dish cover part 21 and a dish sleeve part 22, the dish sleeve part 22 is formed on the outline edge of the lower surface of the dish cover part 21, the dish sleeve part 22 is coaxially sleeved with the convex dish ring 11, and a gas exchange channel is arranged on the dish sleeve part 22; under the state that the culture dish cover 20 and the culture dish body 10 are relatively covered, the culture chamber a is formed on the upper surface side of the culture dish body 11 by the dish cover part 21 and the convex dish ring 11 together, the gas exchange channel is used for communicating the culture chamber a with the outside, and the outside refers to the environment (such as the internal space environment of an incubator) where the whole culture dish is located or placed, so that the gas exchange channel is utilized to improve the gas circulation between the inside of the culture dish and the external environment thereof, shorten the time required by gas balance, maintain the ovum to be always in a stable PH environment, and create conditions for improving the maturation rate of the ovum.

The ovum culture tank 30 is arranged on the upper surface of the culture dish body 10 and is positioned in the culture chamber a, and the ovum culture tank 30 is in a circular truncated cone-shaped tank body structure with the upper bottom radius larger than the lower bottom radius; by utilizing the structural form of the tank body with the big top and the small bottom of the egg culture tank 30, on one hand, the automatic aggregation of the eggs and the culture solution can be realized, the time of the in-vitro operation of the eggs is shortened, and the reduction of the distance between the eggs is automatically realized; on the other hand, in the process of maturation of the ovum, the ovum and granular cells secrete factors for promoting maturation of the ovum, so that the ovum can fully utilize nutrients around the ovum, information and substance exchange among the ovum can be effectively increased in a co-culture mode, and the maturation rate of the ovum is improved.

Referring to fig. 2, one embodiment provides an egg maturation culture dish, further comprising an egg washing tank 40 and an egg transfer tank 50; wherein:

the egg cleaning tank 40 is opened on the upper surface of the culture dish body 10 and is located in the culture chamber a, and the egg cleaning tank 40 is used for accommodating an egg cleaning solution so that the egg can be thoroughly cleaned by the egg cleaning solution in the egg cleaning tank 40 after the egg is moved from the inside to the outside of the body. The egg transfer groove 50 is formed in the upper surface of the culture dish body 10 and is positioned in the culture chamber a, and the egg transfer groove 50 is of a circular truncated cone-shaped groove body structure with the upper bottom radius larger than the lower bottom radius; after the eggs are washed in advance, the eggs are moved into the egg transfer tank 50, and the washed eggs are automatically gathered by utilizing the structural form of the tank body with the big top and the small bottom of the egg transfer tank 50, so that the volume content of the washing liquid can be reduced to the maximum extent during the process of sucking the eggs, and the mutual pollution between the washing liquid (particularly pollutants such as blood and the like contained in the washing liquid) and the culture solution is reduced.

A partition plate 12 is arranged on the upper surface of the culture dish body 10, the partition plate 12 is used for dividing the surface area enclosed by the convex dish ring 11 into a first part and a second part, the ovum culture tank 30 is positioned in the first part, and the ovum cleaning tank 40 and the ovum transfer tank 50 are positioned in the second part. Due to the existence of the isolation plate 12, the pollution of cleaning liquid in the process of in vitro operation of the ovum can be reduced, the risk that a pollution source pollutes culture solution through culture oil is reduced, and favorable structural conditions are created for the subsequent culture and maturation of the ovum.

In this embodiment, the ovum cleaning tank 40 mainly comprises a first cleaning tank 41 and a second cleaning tank 42 which are communicated with each other, and the tank body depth of the first cleaning tank 41 is smaller than that of the second cleaning tank 42 by taking the upper surface of the culture dish body 10 as a reference surface; by using the height difference or depth difference between the two cleaning tanks, in the process of cleaning the eggs, the eggs can be firstly placed in the second cleaning tank 42 for uniform cleaning, then the eggs are sucked one by one and placed in the first cleaning tank 41 for cleaning, and finally the eggs which are finished in the first cleaning tank 41 are placed in the egg transfer tank 50. Thus, the ovum can be thoroughly cleaned to the utmost extent. Of course, in other embodiments, the first cleaning tank 41 and the second cleaning tank 42 may be separated from each other, and the depth of the two tanks may not be limited.

Referring to fig. 1 and 2, in an embodiment, a first attribute marking position 23 is disposed on an upper surface of the dish cover portion 21, a second attribute marking position 13 is disposed on a peripheral wall of the culture dish body 10, and the first attribute marking position 23 and the second attribute marking position 13 are in one-to-one correspondence and are used for marking information of an ovum owner. The attribute mark position can be set into a slot position or a surface area which can be marked by writing characters, attaching labels, printing two-dimensional codes and the like according to actual conditions; by using the correspondence between the first attribute mark position 23 and the second attribute mark position 13, the one-to-one correspondence between the dish bodies 10 and the dish lids 20 can be realized, and the occurrence of an adverse event of confusion between patients or egg owners due to operational errors can be prevented.

Referring to fig. 2, in an embodiment, a positioning bevel 14 is formed on an outer circumferential surface of the convex dish ring 11, the positioning bevel 14 is a plane distributed along an axial direction of the convex dish ring 14, and the dish sleeve portion 22 is adapted to the convex dish ring 11; the gas exchange channel includes a flow gap 24 and a gap flow channel, the flow gap 24 is opened on the lower end surface of the dish sleeve portion 22 and is located on two sides of the circumferential direction of the positioning bevel 14, and the gap flow channel is formed between the outer circumferential wall of the protruding dish ring 11 and the inner circumferential wall of the dish sleeve portion 22. The culture dish cover 20 and the culture dish body 10 can be combined only in a specific angle or specific position mode through the arranged positioning bevel surface 14, the problem that the positions of the culture dish cover 20 and the culture dish body 10 are uncertain due to relative rotation is avoided, favorable conditions are created for forming a gas exchange channel, and meanwhile, the operation efficiency of the whole culture dish can be improved. In this embodiment, the gap flow path may be implemented by providing a corresponding groove body or the like on the inner peripheral wall of the dish sleeve portion 22 and/or the outer peripheral wall of the convex dish ring 11, so that the gap flow path can establish a communication relationship between the culture chamber a and the circulation notch 24. In one embodiment, the circulation notch 24 is a U-like or semicircular structure that is opened on the lower end surface of the cuvette holder 22 and distributed through the inside and outside of the cuvette holder 11, and at least two continuously distributed circulation notches 24 are provided on each side of the positioning bevel 14 in the circumferential direction; in this way, a channel structure similar to an "arch bridge" can be formed at the contour edge of the culture dish cover 20, which facilitates the gas exchange between the interior and the exterior of the culture dish.

Referring to fig. 1 and 3, in one embodiment, the capsule portion 22 further includes a cover-lifting protrusion 25, and the cover-lifting protrusion 25 is located on the outer peripheral wall of the capsule portion 22 and is opposite to the positioning bevel 14. The culture dish cover 20 can be conveniently and quickly covered on the culture dish body 10 by an operator by utilizing the cover-opening protrusion 25, or the culture dish cover 20 can be taken down from the culture dish body 10.

It is right to have used specific individual example above the utility model discloses expound, only be used for helping to understand the utility model discloses, not be used for the restriction the utility model discloses. To the technical field of the utility model technical personnel, the foundation the utility model discloses an idea can also be made a plurality of simple deductions, warp or replacement.

Claims

1. An egg maturation culture dish, comprising:

the culture dish comprises a culture dish body (10), wherein a convex dish ring (11) is arranged on the edge of the outline of the upper surface of the culture dish body (10), and the convex dish ring (11) protrudes out of the upper surface of the culture dish body (10) and is distributed;

the culture dish cover (20), the culture dish cover (20) comprises a dish cover part (21) and a dish sleeve part (22), the dish sleeve part (22) is formed on the outline edge of the lower surface of the dish cover part (21), the dish sleeve part (22) is coaxially sleeved with a protruding dish ring (11), the dish cover part (21) and the protruding dish ring (11) are jointly formed with a culture chamber (a) on the upper surface side of the culture dish body (10), the dish sleeve part (22) is provided with a gas exchange channel, and the gas exchange channel is used for communicating the culture chamber (a) with the outside;

and

the egg culture dish comprises an egg culture tank (30), wherein the egg culture tank (30) is arranged on the upper surface of the culture dish body (10) and is positioned in a culture chamber (a), and the egg culture tank (30) is of a circular truncated cone-shaped groove body structure with the upper bottom radius larger than the lower bottom radius.

2. The egg maturation culture dish of claim 1, further comprising:

the ovum cleaning tank (40), the ovum cleaning tank (40) is arranged on the upper surface of the culture dish body (10) and is positioned in the culture chamber (a), and the ovum cleaning tank (40) is used for containing an ovum cleaning solution; and

the egg transfer groove (50) is formed in the upper surface of the culture dish body (10) and is positioned in the culture chamber (a), and the egg transfer groove (50) is of a circular truncated cone-shaped groove body structure with the upper bottom radius larger than the lower bottom radius;

be provided with division board (12) on the upper surface of culture dish body (10), division board (12) are used for separating the face area that bellied ware ring (11) enclose for first portion and second portion, and ovum culture trough (30) are located the first portion, ovum washing tank (40) and ovum transfer tank (50) are located the second portion.

3. The ovum maturation culture dish according to claim 2, wherein the ovum cleaning tank (40) comprises a first cleaning tank (41) and a second cleaning tank (42), and the tank body depth of the first cleaning tank (41) is smaller than that of the second cleaning tank (42) by taking the upper surface of the culture dish body (10) as a reference surface.

4. The ovum maturation culture dish according to claim 1, wherein a first attribute mark position (23) is arranged on the upper surface of the dish cover part (21), a second attribute mark position (13) is arranged on the circumferential wall of the culture dish body (10), and the first attribute mark position (23) and the second attribute mark position (13) are in one-to-one correspondence and are used for marking information of an ovum owner.

5. The ovum maturation culture dish according to any one of claims 1-4, wherein the outer circumference of the convex dish ring (11) is formed with positioning chamfers (14), the positioning chamfers (14) are planes distributed along the axial direction of the convex dish ring (11), and the dish sleeve part (22) is adapted to the convex dish ring (11);

the gas exchange channel comprises a circulation notch (24) and a gap flow channel, the circulation notch (24) is arranged on the lower end face of the dish sleeve part (22) and located on two circumferential sides of the positioning bevel (14), and the gap flow channel is formed between the outer circumferential wall of the protruding dish ring (11) and the inner circumferential wall of the dish sleeve part (22).

6. An egg maturation culture dish according to claim 5, characterized in that the mantle portion (22) is further provided with cover lifting protrusions (25), which cover lifting protrusions (25) are located on the peripheral wall of the mantle portion (22) and are located opposite the positioning bevels (14).

7. An ovum maturation culture dish according to claim 5, characterized in that the circulation gaps (24) are formed as a U-like or semi-circular structure which opens on the lower end surface of the dish sleeve part (22) and runs through the dish sleeve part (22) and is distributed inside and outside, and that at least two continuously distributed circulation gaps (24) are arranged on each side of the positioning bevel (14) in the circumferential direction.