CN215050378U

CN215050378U - Serial-type in vitro fertilization culture dish

Info

Publication number: CN215050378U
Application number: CN202120766426.3U
Authority: CN
Inventors: 饶金鹏; 金帆; 金敏; 封纯; 邱枫; 毛愉婵; 张颖
Original assignee: Zhejiang University ZJU
Current assignee: Zhejiang University ZJU
Priority date: 2021-04-15
Filing date: 2021-04-15
Publication date: 2021-12-07
Anticipated expiration: 2031-04-15

Abstract

The utility model discloses a serial-type external fertilization culture dish, including splicing six at least function blocks of an organic whole, and detachably connects between two adjacent function blocks, six set up the ovum on the function block respectively and picked up district, ovum rinsing district, sperm egg combining zone, zygote rinsing district, cleavage embryo culture area and blastocyst embryo culture area. Through six at least function blocks that will set up ovum pick-up district, ovum rinsing district, seminal egg combination district, embryonated egg rinsing district, cleavage embryo culture district and blastocyst culture district and establish ties integratively together, make only 1 the culture dish just can carry out effective replacement to a plurality of import culture dishes that present size, shape, specification are different, accomplishes from seminal egg external fertilization to embryo culture's complete set IVF flow, has effectively solved among the prior ART ART laboratory and has adopted a plurality of culture dishes to carry out external fertilization operation and have operated complicacy, the technical problem that work efficiency is low and make mistakes easily, has realized convenient, economic, high-efficient and safe beneficial effect.

Description

Serial-type in vitro fertilization culture dish

Technical Field

The utility model relates to an external reproduction technical field especially relates to a serial-type external fertilization culture dish.

Background

With the release of the comprehensive policy of China 'two children', the number of couples meeting the standards of two children in China is about 9000 ten thousand pairs, wherein 6000 million women are over 35 years old, and along with the superimposed influence of factors such as environmental pollution, high working and living pressure, age increase, bad life style and the like, according to the research report of the current situation of infertility in China issued by China Association of population and the United nations of China Committee of family planning, the incidence rate of infertility in China is 12.5% -15%, and the number of patients is about 4000 million. To achieve the goal of fertility, the most effective way is by Assisted Reproductive Technology (ART) represented by in vitro fertilization-embryo transfer (IVF-ET).

ART involves complex multi-link clinical and laboratory procedures, wherein the laboratory procedures include ovum pickup, semen handling, in vitro fertilization, cleavage stage embryo culture, blastocyst culture, and the like. Currently, each step requires the use of inlet culture dishes with different numbers, sizes, shapes and specifications, and the preparation (preparation of liquid) in advance and the marking of corresponding identity information on dish bodies (dish covers and dish bottoms) are required. Between steps, gametes or embryos often need to be transported from one dish to another, and a rigorous two-person check must be performed.

There are some disadvantages to handling such many different sizes, shapes, and sizes of petri dishes during actual operation in the ART laboratory. First, purchasing such many imported culture dishes increases the cost of IVF operation. Secondly, even if experienced embryologists, need think, calculate the different step of different patients each needs how many different culture dishes before preparing the liquid at every turn for it is not high to prepare liquid efficiency, and to current circular ware, often very hard to carry out reverse mark at the bottom of the ware. When gametes/embryos are transferred from one dish to another dish, along with the increase of the number of infertility patients and the complexity of operation steps, the workload of checking identity information between dishes is increased, and the error risk is increased.

Therefore, the above prior art has at least the following technical problems: in the prior ART, an ART laboratory adopts a plurality of culture dishes to perform in vitro fertilization operation, and has the technical problems of complex operation, low working efficiency and easy error.

SUMMERY OF THE UTILITY MODEL

The embodiment of the application provides a serial-type in vitro fertilization culture dish, has solved that there is the technical problem that the operation is complicated, work efficiency is low and make mistakes easily in the external fertilization operation that ART laboratory adopted a plurality of culture dishes among the prior ART.

For solving above-mentioned technical problem, this application embodiment provides a serial-type external fertilization culture dish, the culture dish is including splicing six at least function blocks as an organic whole, and detachably connects between two adjacent function blocks, six the district is picked up to the ovum, the ovum is rinsed district, sperm egg combines district, zygote rinsing district, cleavage embryo culture district and blastocyst embryo culture district to having set up respectively on the function block.

Further, the culture dish is rectangular, and the ovum pickup area, the ovum rinsing area, the sperm-egg combining area, the fertilized ovum rinsing area, the cleavage embryo culture area and the blastocyst culture area are sequentially arranged.

Furthermore, at least one side of each functional block is provided with a slot or a raised strip, and two adjacent functional blocks are detachably connected through the slots and the buckling of the raised strips.

Furthermore, the ovum picking area comprises a first groove arranged on the upper surface of the first functional block, a first annular wall is arranged on the groove bottom of the first groove, and a second groove is coaxially enclosed on the groove bottom of the first groove by the first annular wall; the first function block is cuboid, and the first groove and the second groove are both circular grooves.

Further, the ovum rinsing area comprises a third groove arranged on the upper surface of the second functional block; the second functional block is in a cuboid shape, and the third groove is a circular groove.

Furthermore, the sperm-egg combining area comprises four fourth grooves arranged at four corners of the upper surface of the third functional block; and the third functional block is in a cuboid shape, and the fourth groove is a circular groove.

Further, the fertilized egg rinsing area comprises a fifth groove arranged on the upper surface of the fourth functional block; and the fourth functional block is in a cuboid shape, and the fifth groove is a circular groove.

Furthermore, the cleavage embryo culture area comprises a sixth groove formed in the upper surface of the fifth function block, a plurality of second annular walls are arranged on the groove bottom of the sixth groove, and a seventh groove is formed in the groove bottom of the sixth groove by the second annular walls in an enclosing manner; and the fifth functional block is in a cuboid shape, and the sixth groove and the seventh groove are both circular grooves.

Further, the blastocyst culturing area comprises an eighth groove formed in the upper surface of the sixth functional block, a plurality of third annular walls are arranged on the groove bottom of the eighth groove, and a ninth groove is formed in the groove bottom of the first groove by the aid of each third annular wall in an enclosing manner; and sixthly, the functional block is in a cuboid shape, and the eighth groove and the ninth groove are circular grooves.

Furthermore, a corner of the upper surface of each functional block is respectively provided with a mark.

One or more technical solutions provided in the embodiments of the present application have at least the following technical effects or advantages:

(1) according to the embodiment of the application, at least six functional blocks provided with an ovum pickup area, an ovum rinsing area, an ovum combining area, a fertilized ovum rinsing area, a cleavage embryo culture area and a blastocyst culture area are connected in series and integrated together, and the functional blocks are detachably connected, so that only 1 culture dish is needed to effectively replace a plurality of import culture dishes with different sizes, shapes and specifications at present, and a complete set of IVF (in vitro fertilization) process from the in vitro fertilization of the sperm and the ovum to the embryo culture is completed, and the consideration and calculation of different culture dishes with different steps of different patients are not needed before liquid preparation; and because the culture dish is the series connection integrated form, consequently greatly reduced with the risk of other patient's culture dishes confusing, only need after having transferred gamete/embryo, with the preceding functional block demolish can, convenient, swift and difficult mistake, effectively solved among the prior ART ART laboratory adopt a plurality of culture dishes to carry out the in vitro fertilization operation and have the technical problem that the operation is complicated, work efficiency is low and easy mistake, realized convenient, economic, high-efficient and safe beneficial effect.

(2) Present commercialized culture dish is mostly 20/package, and to miniature reproductive medicine center, if the patient cycle number is not many, placing time exists the ware body for a long time after unsealing and pollute the risk, the design of a patient's a ware of this application embodiment adopts the independent packaging, and is safer in depositing of consumptive material, and it is little to pollute the risk.

(3) This application embodiment has set up 16 seventh recesses and has been used for the dropping liquid, and at the in-process of joining in marriage the microdroplet, operating personnel uses burette or application of sample rifle all to need control the volume and the interval of drop, and the volume is too big or the interval is too close all causes "even drippage" to take place easily, and circular seventh recess becomes array arrangement, has restricted the size and the position of microdroplet, makes personnel more leisurely at the in-process of joining in marriage the microdroplet, and it is more convenient to join in marriage liquid.

(4) This application embodiment has set up 16 ninth recesses and has been used for the dropping liquid, and at the in-process of joining in marriage the microdroplet, operating personnel uses burette or application of sample rifle all to need control the volume and the interval of drop, and the volume is too big or the interval is too close all causes "even drippage" to take place easily, and circular seventh recess becomes array arrangement, has restricted the size and the position of microdroplet, makes personnel more leisurely at the in-process of joining in marriage the microdroplet, and it is more convenient to join in marriage liquid.

(5) The size of each recess that sets up on the culture dish of this application embodiment is close with the size of current commercial circular dish, accords with operating personnel's operation custom at ordinary times.

(6) The functional blocks of the culture dish are all cuboid, and the grooves arranged on the functional blocks are all square and round grooves, so that redundant areas beside the grooves can be marked in a positive direction.

(7) In the embodiment of the application, a mark is respectively arranged on one corner of the upper surface of each functional block, the mark is a right triangle and is positioned on the upper left corner of the upper surface of each functional block, and the setting of the mark enables an operator to easily judge the left, right, up and down positions of each functional area; different patients can use culture dishes marked by triangular marks in different colors, and visual mutual distinguishing is facilitated.

(8) The blastocyst culture area further comprises a recording area used for marking the identity information of the patient, so that the identity of the patient can be checked accurately, mistakes are avoided, and the checking safety is further improved.

In summary, the culture dish of the embodiment of the application has the advantages of convenient operation, high efficiency in use, safety checking, pollution avoidance and cost economy.

Drawings

FIG. 1 is a schematic diagram of an overall structure of a tandem type in vitro fertilization culture dish according to an embodiment of the present application;

FIG. 2 is a top view of a tandem type in vitro fertilization culture dish according to an embodiment of the present application;

fig. 3 is a side view of an in-line in vitro fertilization culture dish according to an embodiment of the present application.

Detailed Description

In order to solve the technical problems, the technical scheme provided by the application has the following general idea: at least six functional blocks provided with an ovum pickup area, an ovum rinsing area, an sperm and ovum combining area, a fertilized ovum rinsing area, a cleavage embryo culture area and a blastocyst culture area are integrated in series and are detachably connected with one another, so that a plurality of imported culture dishes with different sizes, shapes and specifications at present can be effectively replaced by only 1 culture dish, the whole set of IVF flow from sperm and ovum in-vitro fertilization to embryo culture is completed, and the consideration and calculation of different culture dishes required by different steps of different patients before liquid preparation are not needed; and because the culture dish is the series connection integrated form, consequently greatly reduced with the risk of other patient's culture dishes confusing, only need after having transferred gamete/embryo, with the preceding functional block demolish can, convenient, swift and difficult mistake, effectively solved among the prior ART ART laboratory adopt a plurality of culture dishes to carry out the in vitro fertilization operation and have the technical problem that the operation is complicated, work efficiency is low and easy mistake, realized convenient, economic, high-efficient and safe beneficial effect.

The technical solutions of the present application are described in detail below with reference to the drawings and specific embodiments, and it should be understood that the specific features in the embodiments and examples of the present application are detailed descriptions of the technical solutions of the present application, and are not limitations of the technical solutions of the present application, and the technical features in the embodiments and examples of the present application may be combined with each other without conflict.

Fig. 1 to 3 are a schematic overall structure diagram, a top view and a side view of a serial in vitro fertilization culture dish according to an embodiment of the present application, and as shown in fig. 1 to 3, the culture dish 1 includes at least six functional blocks that can be spliced into a whole, and two adjacent functional blocks are detachably connected to each other; the six functional blocks are respectively provided with an ovum picking area 11, an ovum rinsing area 12, an sperm-egg combining area 13, a fertilized egg rinsing area 14, a cleavage embryo culture area 15 and a blastocyst culture area 16.

Specifically, the ovum pickup area 11 is used for picking up the ovum, the ovum rinsing area 12 is used for semen treatment and rinsing the ovum, the sperm and ovum combining area 13 is used for in vitro fertilization, the fertilized ovum rinsing area 14 is used for rinsing the fertilized ovum, the cleavage embryo culture area 15 is used for culturing the embryo at the cleavage stage, and the blastocyst culture area 16 is used for culturing the blastocyst, so that the culture dish 1 can complete all the steps of in vitro fertilization operation in one dragon.

In the embodiment of the application, at least six functional blocks provided with an ovum pickup area 11, an ovum rinsing area 12, an ovum combining area 13, a fertilized ovum rinsing area 14, an cleavage embryo culture area 15 and a blastocyst culture area 16 are connected in series and integrated together, and the functional blocks are detachably connected, so that a plurality of imported culture dishes with different sizes, shapes and specifications at present can be effectively replaced by only 1 culture dish, a complete set of IVF (in vitro fertilization) process from the sperm ovum in vitro fertilization to the embryo culture is completed, and the consideration and calculation of different culture dishes for different steps of different patients before liquid preparation are not needed; and because the culture dish is the series connection integrated form, consequently greatly reduced with the risk of other patient's culture dishes confusing, only need after having transferred gamete/embryo, with the preceding functional block demolish can, convenient, swift and difficult mistake, effectively solved among the prior ART ART laboratory adopt a plurality of culture dishes to carry out the in vitro fertilization operation and have the technical problem that the operation is complicated, work efficiency is low and easy mistake, realized convenient, economic, high-efficient and safe beneficial effect.

Specifically, even if experienced embryologist, need think, calculate the different step of different patients and respectively need how many different culture dishes of many before preparing liquid at every turn, and the utility model discloses then carry out "fool's" design according to IVF operation full flow, as long as take out the culture dish of same number according to corresponding patient number can, convenient, economy.

In addition, current commercialized culture dish is mostly 20/package, and to miniature reproductive medicine center, if the patient cycle number is not many, placing time exists the ware body for a long time after unsealing and pollutes the risk, this application embodiment the culture dish adopts the independent packaging for the design of a piece of ware of a patient, and is safer in depositing of consumptive material, and it is little to pollute the risk.

In an embodiment of this application, culture dish 1 is the cuboid form, and is 370mm from the length of left hand right side, wide 70mm, high 10mm, just the ovum pick up the district 11 the ovum rinse district 12 the sperm ovum combines the district 13 the fertilized egg rinse district 14 the cleavage embryo culture district 15 with blastocyst embryo culture district 16 certainly the left end right end of culture dish 1 is arranged in proper order to accord with the order of body IVF operation.

For convenience, the functional blocks on the culture dish 1 are marked as a first functional block, a second functional block, a third functional block, a fourth functional block, a fifth functional block and a sixth functional block from left to right in sequence.

In this application embodiment, connect through connecting device 17 detachably between two adjacent function blocks, connecting device 17 includes slot 172 and the sand grip 171 of looks buckle, at least one side of function block is equipped with slot 172 or sand grip 171, slot 172 with sand grip 171 snap-fit, specifically, be located the leftmost end the left end face of district 11 is picked up to the ovum and be located the rightmost end the right end face of area 16 is cultivateed to the blastocyst does not set up slot 172 or sand grip 171. Specifically, each functional block has a rectangular parallelepiped shape, the protrusion 171 has a rectangular parallelepiped shape, and the slot 172 has a rectangular long slot.

In one embodiment of the present application, the egg pickup zone 11 comprises a first groove 18 disposed on the upper surface of a first one of the functional blocks, the length of the first one of the functional blocks being 70 mm; a first annular wall 181 is arranged in the first groove 18, a second groove is coaxially enclosed on the bottom of the first groove 18 by the first annular wall 181, the first groove 18 is a circular groove, the diameter of the first groove 18 is 60mm, and the depth of the first groove 18 is 9 mm; the diameter of the first annular wall 181 is 35mm, the height thereof is 6mm, and the thickness thereof is 1mm, and the first groove 18 is used for containing the gametophyte external treatment fluid.

In an embodiment of the present application, the ovum rinsing area 12 includes setting up the second the third recess 19 on the function block upper surface, and the second the length of function block is 45mm, and third recess 19 is the circular slot, and third recess 19's diameter is 35mm, the degree of depth is 9mm, just third recess 19 sets up the second the centre of function block upper surface, third recess 19 are used for holding the outer treatment fluid of gametophyte.

In an embodiment of the present application, the sperm-egg combining area 13 includes four fourth grooves 20 disposed at four corners of the upper surface of the third functional block, the length of the third functional block is 70mm, the fourth grooves 20 are circular grooves, the diameter of the fourth grooves 20 is 15mm, the depth of the fourth grooves 20 is 9mm, and the fourth grooves 20 are used for containing the culture solution for in vitro fertilization.

In an embodiment of the present application, the fertilized egg rinsing area 14 includes a fifth groove disposed on the upper surface of the fourth functional block, and the fourth length of the functional block is 45mm, the fifth groove is a circular groove, the diameter of the fifth groove is 35mm, the depth of the fifth groove is 9mm, the fifth groove is disposed in the fourth middle of the upper surface of the functional block, and the fifth groove is used for containing the cleavage culture solution.

In an embodiment of the present application, the cleavage embryo culture area 15 is including setting up fifth sixth recess on the function block upper surface, the fifth the length of function block is 70mm, and the sixth recess is the circular slot, and the degree of depth is 9mm, be equipped with a plurality of second annular wall 182 on the tank bottom of sixth recess, each second annular wall 182 is in enclose respectively on the tank bottom of sixth recess and close a seventh recess, the diameter of second annular wall 182 is 5mm, highly is 3mm, thickness is 1mm, just be equipped with 16 seventh recesses in the sixth recess, every row of 5 in the first three rows, last row just sets up between two parties.

Specifically, the sixth groove is used for containing the cleavage culture solution, 16 seventh grooves are used for the dropping liquid, and in the process of dispensing the microdroplets, an operator needs to control the volume and the distance of the dropping liquid by using a dropper or a sample adding gun, the overlarge volume or the too close distance easily cause the 'continuous dropping' to occur, the circular seventh grooves are arranged in an array mode, the size and the position of the microdroplets are limited, so that the personnel can more easily dispense the microdroplets, and the liquid dispensing is more convenient.

In an embodiment of the present application, the blastocyst culturing area 16 includes that the setting is in the sixth eighth recess on the function block upper surface, the sixth the length of function block is 70mm, and the eighth recess degree of depth is 9mm, be equipped with a plurality of third annular wall on the tank bottom of eighth recess, each the third annular wall is in enclose respectively on the tank bottom of first recess and close a ninth recess, the diameter of third annular wall is 5mm, highly is 3mm, thickness is 1mm, just be equipped with 16 ninth recesses in the eighth recess, every row of 5 in the first three rows, last row 1 and set up between two parties.

Specifically, be used for holding blastocyst culture solution in the eighth recess, 16 ninth recesses are used for the dropping liquid, and at the in-process of joining in marriage the microdroplet, operating personnel uses burette or application of sample rifle all to need control the volume and the interval of dropping liquid, and the volume is too big or the interval is too close all causes "even drop" to take place easily, and circular seventh recess becomes array and arranges, has carried out the restriction to the size and the position of microdroplet, makes personnel more leisurely, and it is more convenient to join in marriage the liquid at the in-process of joining in marriage the microdroplet.

The size of each recess that sets up on the culture dish of this application embodiment is close with the size of current commercial circular dish, accords with operating personnel's operation custom at ordinary times. In addition, to current circular ware, it is often very arduous to carry out reverse mark at the ware bottom, each functional block of culture dish described in the embodiment of this application is the cuboid form, and the recess that sets up on it is square circular groove, therefore the other unnecessary other region of groove of recess can be made forward mark.

In an embodiment of the application, a mark 21 is respectively arranged on one corner of the upper surface of each functional block, the mark 21 is a right triangle, the mark 21 is positioned on the upper left corner of the upper surface of each functional block, and the marks are arranged so that an operator can easily judge the left, right, up and down positions of each functional area; different patients can use culture dishes marked by triangular marks in different colors, and visual mutual distinguishing is facilitated.

In one embodiment of the present application, the blastocyst cultivation area 16 further comprises a recording area 22 for marking the identity information of the patient, thereby facilitating the accurate verification of the identity of the patient, avoiding errors, and further improving the security of the verification.

The application method of the culture dish in the embodiment of the application is as follows:

(1) according to the IVF starting cycle number of the next day, taking out a corresponding number of culture dishes 1 from the packaging bag, marking the identity information of the patient on a recording area 22 of the culture dishes 1, adding a gametophyte external treatment solution into a first groove 18 of an ovum pickup area 11 and a third groove 19 of an ovum rinsing area 12, adding an in vitro fertilization culture solution into four fourth grooves 20 of a sperm-ovum binding area 13, adding a cleavage culture solution into a fifth groove of a fertilized ovum rinsing area 14 and a sixth groove of a cleavage embryo culture area 15, adding a blastocyst culture solution into an eighth groove of a blastocyst culture area 16, and performing surface paraffin oil covering treatment on all the culture solutions;

(2) splitting a cutting 171 on the right side of the ovum rinsing area 12 and a slot 172 on the left side of the sperm and ovum combining area 13, putting two functional blocks of the ovum picking area 11 and the ovum rinsing area 12 into a 37 ℃ incubator without CO2 for overnight balance, and putting the rest culture dishes 1 (the functional blocks of the sperm and ovum combining area 13, the fertilized ovum rinsing area 14, the cleavage embryo culture area 15 and the blastocyst culture area 16) into a 37 ℃ incubator containing CO2 with the percentage concentration of 6% for overnight balance;

(3) the picking and rinsing of the ovum are respectively completed in the ovum picking area 11 and the ovum rinsing area 12, at the moment, the culture dish 1 (the functional blocks in which the sperm and ovum combining area 13, the fertilized ovum rinsing area 14, the cleavage embryo culture area 15 and the blastocyst culture area 16 are positioned) is taken out, and after the right-angled triangle mark 21 with the same color and the identity mark of the recording area 22 of the culture dish 1 are arranged at the upper left corner of each functional block for checking, the ovum is transferred to the sperm and ovum combining area 13;

(4) and (3) transferring the fertilized eggs or embryos to the right functional block in sequence, and detaching the left functional block through the concave-convex structure 17 when finishing transferring, namely detaching the sperm-egg combining area 13, the fertilized egg rinsing area 14 and the cleavage embryo culture area 15 in sequence, and reserving the right part until all IVF processes are completed.

The technical scheme in the embodiment of the application at least has the following technical effects or advantages:

(1) in the embodiment of the application, at least six functional blocks provided with an ovum pickup area 11, an ovum rinsing area 12, an ovum combining area 13, a fertilized ovum rinsing area 14, an cleavage embryo culture area 15 and a blastocyst culture area 16 are connected in series and integrated together, and the functional blocks are detachably connected, so that a plurality of imported culture dishes with different sizes, shapes and specifications at present can be effectively replaced by only 1 culture dish, a complete set of IVF (in vitro fertilization) process from the sperm ovum in vitro fertilization to the embryo culture is completed, and the consideration and calculation of different culture dishes for different steps of different patients before liquid preparation are not needed; and because the culture dish is the series connection integrated form, consequently greatly reduced with the risk of other patient's culture dishes confusing, only need after having transferred gamete/embryo, with the preceding functional block demolish can, convenient, swift and difficult mistake, effectively solved among the prior ART ART laboratory adopt a plurality of culture dishes to carry out the in vitro fertilization operation and have the technical problem that the operation is complicated, work efficiency is low and easy mistake, realized convenient, economic, high-efficient and safe beneficial effect.

(7) In the embodiment of the application, a mark 21 is respectively arranged on one corner of the upper surface of each functional block, the mark 21 is a right triangle, the mark 21 is positioned on the upper left corner of the upper surface of each functional block, and the marks are arranged so that an operator can easily judge the left, right, up and down positions of each functional area; different patients can use culture dishes marked by triangular marks in different colors, and visual mutual distinguishing is facilitated.

(8) The blastocyst culturing area 16 of the present embodiment further includes a recording area 22 for marking the identity information of the patient, so as to facilitate the accurate checking of the identity of the patient, avoid errors, and further improve the checking safety.

It should be understood that the terms of orientation of up, down, left, right, front, back, top, bottom, etc., referred to or may be referred to in this specification, are defined relative to the configuration shown in the drawings, and are relative terms, and thus may be changed accordingly depending on the position and the use state of the device. Therefore, these and other directional terms should not be construed as limiting terms.

While the foregoing is directed to the preferred embodiment of the present application, and not to the limiting thereof in any way and any way, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims. Those skilled in the art can make various changes, modifications and equivalent arrangements to those skilled in the art without departing from the spirit and scope of the present application; moreover, any equivalent alterations, modifications and variations of the above-described embodiments according to the spirit and techniques of this application are intended to be within the scope of the claims of this application.

Claims

1. The utility model provides a serial-type external fertilization culture dish, its characterized in that, the culture dish is including splicing six at least function blocks as an organic whole, and detachably connects between two adjacent function blocks, six the function block has set up respectively that the ovum picks up district, ovum rinsing district, sperm and egg combination district, fertilized egg rinsing district, cleavage embryo culture area and blastocyst culture area.

2. The in-vitro fertilization culture dish according to claim 1, wherein the culture dish is rectangular, and the ovum pickup area, the ovum rinsing area, the sperm-ovum binding area, the fertilized ovum rinsing area, the cleavage embryo culture area and the blastocyst culture area are arranged in this order.

3. The in-vitro fertilization culture dish according to claim 1, wherein at least one side of each functional block is provided with a slot or a rib, and two adjacent functional blocks are detachably connected through the snap-fit engagement of the slot and the rib.

4. The in-vitro fertilization culture dish of claim 1, wherein the egg pickup area comprises a first groove disposed on an upper surface of a first one of the function blocks, a first annular wall disposed on a bottom of the first groove, the first annular wall coaxially enclosing a second groove on the bottom of the first groove; the first function block is cuboid, and the first groove and the second groove are both circular grooves.

5. The in-vitro fertilization culture dish according to claim 1, wherein the egg rinsing zone comprises a third groove provided on an upper surface of a second one of the functional blocks; the second functional block is in a cuboid shape, and the third groove is a circular groove.

6. The in-vitro fertilization culture dish according to claim 1, wherein the sperm-egg binding region comprises four fourth grooves disposed at four corners of the upper surface of the third functional block; and the third functional block is in a cuboid shape, and the fourth groove is a circular groove.

7. The in-line in vitro fertilization culture dish of claim 1, wherein the zygote rinsing zone comprises a fifth groove provided on an upper surface of a fourth of the functional blocks; and the fourth functional block is in a cuboid shape, and the fifth groove is a circular groove.

8. The in-vitro fertilization culture dish of claim 1, wherein the blastocyst culture section comprises a sixth groove formed on the upper surface of the fifth functional block, and a plurality of second annular walls are formed on the bottom of the sixth groove, and each of the second annular walls encloses a seventh groove on the bottom of the sixth groove; and the fifth functional block is in a cuboid shape, and the sixth groove and the seventh groove are both circular grooves.

9. The in-vitro fertilization culture dish of claim 1, wherein the blastocyst cultivation area comprises an eighth groove formed on the upper surface of the sixth functional block, a plurality of third annular walls are formed on the bottom of the eighth groove, and each third annular wall encloses a ninth groove on the bottom of the first groove; and sixthly, the functional block is in a cuboid shape, and the eighth groove and the ninth groove are circular grooves.

10. The in-vitro fertilization culture dish according to claim 1, wherein a corner of the upper surface of each functional block is marked.