CN212595046U

CN212595046U - Totally-enclosed reaction liquid preparation device

Info

Publication number: CN212595046U
Application number: CN202021526675.7U
Authority: CN
Inventors: 刘梅; 陈俊飞; 万茂坤; 郑典苏; 唐大运; 郝效禹; 李国平; 吴济周
Original assignee: Wuxi Kezhida Technology Co ltd
Current assignee: Wuxi Kezhida Technology Co ltd
Priority date: 2020-07-28
Filing date: 2020-07-28
Publication date: 2021-02-26
Anticipated expiration: 2030-07-28

Abstract

The utility model relates to a device is prepared to totally-enclosed reaction liquid, including reagent chamber shell, be equipped with connect the through-hole on the reagent chamber shell, reagent chamber, waste liquid chamber and liquid interchanger installation position, reagent chamber bottom is equipped with reagent chamber bottom hole, the upper cover is installed to reagent chamber top, set up piston or gasbag, sub-unit connection linker in the liquid interchanger. The utility model has simple structure and small occupied space; the working time is short: the preparation of the reaction liquid can be completed within 2min, compared with manual operation, the speed can be improved by 5-10 times, the preparation is fully automatic, and the operation is convenient: sample dilution, volume quantification, reagent mixing and the like are integrated in one card box, various reaction liquid components do not need to be added manually, and the full automation of the whole process is realized.

Description

Totally-enclosed reaction liquid preparation device

Technical Field

The utility model belongs to the technical field of biological assay, a totally-enclosed reaction liquid preparation device is related to.

Background

The preparation of the reaction solution for nucleic acid detection generally requires diluting a nucleic acid sample to a concentration required for detection, and then sucking a certain amount of the nucleic acid sample, a reaction buffer solution, an enzyme solution and a primer solution according to the detection requirement, and uniformly mixing. At present, nucleic acid detection is a common detection method, but the preparation of a nucleic acid detection reaction solution is mainly performed manually, the whole process is finished by professional technicians, and meanwhile, a separate solution preparation laboratory is required to prevent cross contamination, so that the nucleic acid detection time is prolonged, and the popularization and application of a nucleic acid detection technology are limited.

Disclosure of Invention

An object of the utility model is to provide a totally enclosed reaction liquid preparation device can solve foretell problem.

According to the utility model provides a technical scheme: a fully-closed reaction liquid preparation device comprises a reagent cavity shell, wherein a connecting through hole, a reagent cavity, a waste liquid cavity and a liquid exchanger are arranged on the reagent cavity shell, a reagent cavity bottom hole is formed in the bottom of the reagent cavity, an upper cover is arranged above the reagent cavity, a piston or an air bag is arranged in the liquid exchanger, and the lower portion of the liquid exchanger is connected with a communicating vessel; the slider is driven by drive element, drive element includes the mounting bracket, the vertical installation motor in mounting bracket upper portion, guide rail slidable mounting slider is passed through to the mounting bracket side, motor output shaft connects the lead screw, the screw seat is established to the lead screw overcoat, screw seat one end is connected the slider, the other end passes through the transfer line and connects piston or gasbag.

As a further improvement of the utility model, the upper cover is provided with one or more sample adding holes.

As a further improvement, the vertical intercommunicating pore that runs through in the liquid exchanger outside, the intercommunication groove has been seted up on the linker, intercommunication groove one end with liquid exchanger's inner chamber intercommunication, the other end with the intercommunicating pore is linked together.

As a further improvement of the utility model, the communication hole and the reagent cavity bottom hole are on the same diameter.

As a further improvement of the utility model, the reagent cavity comprises a sample cavity, a diluent cavity, a quantitative cavity and a mixing cavity; the dosing chamber is adjacent to the waste chamber and may be interconnected.

As a further improvement of the present invention, the sample chamber, the diluent chamber, the quantitative chamber and the mixing chamber may be one or more.

As a further improvement of the utility model, open sample chamber bottom has sample chamber bottom hole, open diluent chamber bottom has diluent chamber bottom hole, be equipped with quantitative chamber bottom hole and quantitative chamber inlet channel in the ration chamber, open the hybrid chamber bottom has the hybrid chamber bottom hole.

As a further improvement of the utility model, the quantitative cavity bottom hole is located quantitative cavity bottom, quantitative cavity inlet channel liquid outlet is located quantitative cavity upper portion, quantitative cavity inlet channel goes into the liquid mouth and is located quantitative cavity bottom.

As a further improvement of the present invention, the communicating vessel is connected to the liquid exchanger in an integrated manner.

As a further improvement of the utility model, the mixing cavity can be internally pre-filled with a liquid reagent or a solid freeze-drying reagent.

The positive progress effect of this application lies in:

1. the utility model has simple structure and small occupied space; the working time is short: the preparation of the reaction liquid can be completed within 2min, and compared with manual operation, the speed can be improved by 5-10 times.

2. The utility model discloses full-automatic preparation, convenient operation: sample dilution, volume quantification, reagent mixing and the like are integrated in one card box, various reaction liquid components do not need to be added manually, and the full automation of the whole process is realized.

3. The utility model discloses closed card box: only need add the sample, all other operations are in airtight card box, multiple anti-pollution and prevent leaking reagent, reduce nucleic acid aerosol pollution risk, no test sample and reagent leak risk.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a top view of the reagent chamber housing 100 of the present invention.

Fig. 3 is a top view of the upper cover 200 of the present invention.

Fig. 4 is a structure view of the base plate of the liquid exchanger 400 and the communicating vessel 300 according to the present invention.

Detailed Description

The following description will further describe embodiments of the present invention with reference to the accompanying drawings.

In fig. 1 to 4, the reagent chamber housing 100, the sample chamber 101, the diluent chamber 102, the quantitative chamber inlet channel 103, the quantitative chamber 104, the quantitative chamber bottom hole 105, the mixing chamber 106, the upper cover 200, the communicating vessel 300, the liquid exchanger 400, and the like are included.

As shown in fig. 1-4, the present invention relates to a fully-enclosed reaction solution preparing device, which comprises a reagent chamber shell 100, an upper cover 200, a communicating vessel 300, and a liquid exchanger 400; the reagent chamber shell 100 is provided with an instrument connecting through hole 109 for connecting the reagent chamber shell 100 with an experimental instrument, a reagent chamber and a waste liquid chamber 110 are arranged in the reagent chamber shell 100, an upper cover 200 is arranged on the reagent chamber shell 100, a sample adding hole 201 is arranged on the upper cover 200, a liquid exchanger 400 is arranged in the reagent chamber shell 100, a piston or an air bag is arranged in the liquid exchanger 400, a communicating vessel 300 is arranged below the liquid exchanger 400, a communicating groove 301 is arranged on the communicating vessel 300, one end of the communicating groove 301 is communicated with an inner chamber of the liquid exchanger 400, the other end of the communicating groove 301 is connected with a communicating hole 401, the communicating hole 401 and a bottom hole of the reagent chamber are on the same diameter, and the inner chamber of the liquid exchanger 400 can be selectively communicated with the bottom hole.

The reagent cavity at least comprises a sample cavity 101, a diluent cavity 102, a quantitative cavity 104 and a mixing cavity 106, wherein the bottom of each cavity is provided with a reagent cavity bottom hole which can be communicated with the inner cavity of the liquid exchanger 400 through a communicating vessel 300, and the sample cavity 101 corresponds to the position of the sample adding hole 201.

A connection through hole 109 for connecting with the laboratory instrument is provided inside the reagent chamber housing 100.

The reagent chamber housing 100 has an inverted button and the communicator 300 is fastened by the housing inverted button.

The dosing chamber 104 is adjacent to the waste chamber 110 and may be in communication with each other.

The dosing chamber 104 comprises a dosing chamber bottom aperture 105 and a dosing chamber inlet channel 103. A quantitative cavity bottom hole 105 is positioned at the bottom of the quantitative cavity 104, a quantitative cavity liquid inlet channel 103 is arranged in the quantitative cavity 104, a liquid inlet of the quantitative cavity liquid inlet channel 103 is positioned at the bottom of the quantitative cavity 104, and a liquid outlet of the quantitative cavity liquid inlet channel 103 is positioned at the upper part of the quantitative cavity 104.

The communicator 300 is freely rotatable.

The communicating vessel 300 and the liquid exchanger 400 may be integrally connected.

The diluent chamber 102 is filled with diluent.

The mixing chamber 106 may be pre-filled with a liquid reagent or a solid lyophilized reagent.

The utility model discloses in, different reagents can be pre-installed in inclosed reagent intracavity to can realize not having the leakage.

The reagent chamber is connected with the inner chamber of the liquid exchanger 400 through the communicating vessel 300, the liquid exchanger 400 can be connected with different reagent chambers by rotating the communicating vessel 300, and liquid can be transferred back and forth between the reagent chamber and the inner chamber of the liquid exchanger 400 by the up and down movement of the piston or the air bag.

The piston or the air bag is driven by a driving element 500, the driving element 500 comprises a mounting frame 501, a motor 502 is vertically mounted on the upper portion of the mounting frame 501, a sliding block is slidably mounted on the side surface of the mounting frame 501 through a guide rail, an output shaft of the motor 502 is connected with a lead screw 503, a screw seat 504 is sleeved outside the lead screw 503, one end of the screw seat 504 is connected with the sliding block, and the other end of the screw seat is connected with the piston or the.

The mounting bracket 501 is mounted on top of the liquid exchanger 400.

The motor 502 drives the nut seat 504 to move up and down through the screw rod 503, so as to realize the ascending or descending of the piston or the air bag.

The driving element can also be a micro air cylinder or a micro oil cylinder.

1. Sample dilution

Rotating the communicating vessel 300 to communicate the sample chamber bottom hole 111 with the communicating vessel 300, moving the piston or the air bag up to transfer the sample to the liquid exchanger 400, rotating the communicating vessel 300 to communicate the diluent chamber bottom hole 112 with the communicating vessel 300, moving the piston or the air bag down to transfer the sample in the liquid exchanger 400 to the diluent chamber 102, then moving the piston or the air bag up and down to and fro 5 times to fully mix the sample and the diluent.

2. Volume quantification

The communicating vessel 300 is rotated to communicate the bottom hole 112 of the diluting liquid chamber with the communicating vessel 300, the piston or the air bag is raised to transfer the liquid in the diluting liquid chamber 102 to the liquid exchanger 400, the communicating vessel 300 is rotated to communicate the quantitative liquid inlet channel 103 with the communicating vessel 300, the piston or the air bag is lowered to transfer the liquid in the liquid exchanger 400 from the upper part of the quantitative chamber 104 to the quantitative chamber 104, and the surplus liquid overflows from the quantitative chamber 104 and flows into the waste liquid chamber 110.

3. Transferring a quantitative solution

The communicating vessel 300 is rotated to communicate the bottom hole 105 of the quantitative determination chamber with the communicating vessel 300, and the piston or the air bag is raised to transfer the liquid in the quantitative determination chamber 104 to the liquid exchanger 400.

4. Dissolving freeze-drying reagent

And rotating the communicating vessel 300 to communicate the bottom hole 107 of the mixing cavity with the communicating vessel 300, descending the piston or the air bag, transferring the liquid in the liquid exchanger 400 to the reagent tube in the mixing cavity 106, then ascending the piston or the air bag, and reciprocating up and down for 5 times to fully dissolve and uniformly mix the freeze-dried reagent.

5. Subsequent treatment of the reaction solution

The piston or the air bag ascends to transfer the liquid in the reagent tube to the liquid exchanger 400, the communicating vessel 300 rotates to communicate the sample chamber bottom hole 111 with the communicating vessel 300, and then the piston or the air bag descends to transfer the liquid in the liquid exchanger 400 to the sample chamber 101. The prepared reaction solution in the sample cavity 101 can be directly taken out from the sample hole for subsequent treatment.

The utility model discloses in, other reservation chambeies launch when having the needs.

The utility model discloses in, the inner chamber that realizes liquid exchanger 400 through rotatory communicating vessel 300 links to each other with each reagent chamber, through the up-and-down motion of piston or gasbag, realizes the round trip transfer of liquid at reagent chamber and liquid exchanger 400 inner chamber.

The utility model discloses in, can realize the fixed and intercommunication to the reagent chamber of reagent chamber shell 100 with the help of module assembly (containing fixed chamber shell module, piston or gasbag drive assembly, linker drive assembly). The transfer of liquid between the reagent chamber and the liquid exchanger 400 is achieved by rotating the communicating vessel 300 and driving the piston or bladder up and down. The fully-closed automatic preparation function of the nucleic acid detection reaction solution can be realized through the functions.

The utility model discloses in, can realize the follow-up treatment to reaction liquid through increasing other structures or device, like heating, amplification and detection etc..

Claims

1. A totally-enclosed reaction liquid preparation device is characterized in that: the device comprises a reagent cavity shell (100), wherein a connecting through hole (109), a reagent cavity, a waste liquid cavity (110) and a liquid exchanger (400) are arranged on the reagent cavity shell (100), a reagent cavity bottom hole is formed in the bottom of the reagent cavity, an upper cover (200) is installed above the reagent cavity, a piston or an air bag is arranged in the liquid exchanger (400), and the lower part of the liquid exchanger is connected with a communicating vessel (300); the slider is driven by drive element (500), drive element (500) include mounting bracket (501), vertical installation motor (502) in mounting bracket (501) upper portion, guide rail slidable mounting slider is passed through to mounting bracket (501) side, motor (502) output shaft is connected lead screw (503), screw seat (504) are established to lead screw (503) overcoat, screw seat (504) one end is connected the slider, the other end passes through the transfer line and connects piston or gasbag.

2. The totally enclosed reaction liquid preparation apparatus according to claim 1, characterized in that: one or more sample adding holes (201) are arranged on the upper cover (200).

3. The totally enclosed reaction liquid preparation apparatus according to claim 1, characterized in that: the outer side of the liquid exchanger (400) vertically penetrates through a communication hole (401), a communication groove (301) is formed in the communicator (300), one end of the communication groove is communicated with the inner cavity of the liquid exchanger (400), and the other end of the communication groove is communicated with the communication hole (401).

4. The totally enclosed reaction liquid preparation apparatus according to claim 3, characterized in that: the communication hole (401) and the reagent cavity bottom hole are on the same diameter.

5. The totally enclosed reaction liquid preparation apparatus according to claim 1, characterized in that: the reagent cavity comprises a sample cavity (101), a diluent cavity (102), a quantitative cavity (104) and a mixing cavity (106); the dosing chamber (104) is adjacent to the waste chamber (110) and may be in communication with each other.

6. The totally enclosed reaction liquid preparation apparatus according to claim 5, characterized in that: the sample chamber (101), the diluent chamber (102), the quantitative chamber (104) and the mixing chamber (106) may be one or more.

7. The totally enclosed reaction liquid preparation apparatus according to claim 5, characterized in that: open sample chamber bottom hole (111) sample chamber (101) bottom, open diluent chamber (102) bottom has diluent chamber bottom hole (112), be equipped with ration chamber bottom hole (105) and ration chamber inlet channel (103) in ration chamber (104), open mixing chamber bottom hole (107) mixing chamber (106) bottom.

8. The totally enclosed reaction liquid preparation apparatus according to claim 7, characterized in that: quantitative chamber bottom outlet (105) are located quantitative chamber (104) bottom, quantitative chamber inlet channel (103) liquid outlet is located quantitative chamber (104) upper portion, quantitative chamber inlet channel (103) income liquid mouth is located quantitative chamber (104) bottom.

9. The totally enclosed reaction liquid preparation apparatus according to claim 1, characterized in that: the communicating vessel (300) and the liquid exchanger (400) are integrally connected.

10. The totally enclosed reaction liquid preparation apparatus according to claim 5, characterized in that: the mixing chamber (106) may be pre-filled with a liquid reagent or a solid lyophilized reagent.