CN212586318U

CN212586318U - Pressure stabilizing device, glycosylated hemoglobin analysis system and multifunctional all-in-one machine

Info

Publication number: CN212586318U
Application number: CN202021555169.0U
Authority: CN
Inventors: 徐文超
Original assignee: Shenzhen Dymind Biotechnology Co Ltd
Current assignee: Shenzhen Dymind Biotechnology Co Ltd
Priority date: 2020-07-31
Filing date: 2020-07-31
Publication date: 2021-02-23
Anticipated expiration: 2030-07-31

Abstract

The utility model relates to a voltage regulator device, glycated haemoglobin analytic system and multifunctional integrated machine. Pressure stabilizer includes infusion mechanism, pressure measurement mechanism and exhaust mechanism, and infusion mechanism is used for carrying the eluent through degasification, and pressure measurement mechanism locates between infusion mechanism and the exhaust mechanism, and pressure measurement mechanism is used for detecting the pressure of eluent, and when the pressure of the eluent that pressure measurement mechanism detected was less than the pressure threshold value, it contains the bubble in the eluent to show, exhausts through exhaust mechanism, can eliminate the bubble in the eluent, can also play the effect of adjusting elution hydraulic pressure. The pressure stabilizing device is applied to a glycosylated hemoglobin analysis system and a multifunctional all-in-one machine, so that the measurement precision of the glycosylated hemoglobin can be improved.

Description

Pressure stabilizing device, glycosylated hemoglobin analysis system and multifunctional all-in-one machine

Technical Field

The utility model relates to a blood analysis technical field, concretely relates to voltage regulator device, glycated haemoglobin analytic system and multifunctional integrated machine.

Background

Glycated hemoglobin is a compound formed by non-enzymatic action of hemoglobin and carbohydrates in erythrocytes in blood, and the concentration of glycated hemoglobin in human blood is an important clinical indicator for diagnosis of diabetes.

At present, there are many methods for measuring glycated hemoglobin, including manual microcolumn method, immunoassay method, electrophoresis mass spectrometry, ion exchange chromatography, etc., among which the most common method is ion exchange chromatography. The ion exchange chromatography has strict requirements on the flow rate and pressure of the eluent, and when the flow rate is unstable and the pressure fluctuates obviously, the dissolved gas in the eluent is easily converted into bubbles, and the bubbles can seriously influence the separation and detection of the hemoglobin. In addition, in the degassing stage, there may be situations where degassing is incomplete and where gas bubbles are still present in the eluent, which can reduce the measurement accuracy and even lead to erroneous measurement results.

SUMMERY OF THE UTILITY MODEL

In view of this, the present invention provides a pressure stabilizer, a glycated hemoglobin analysis system and a multifunctional integrated machine, which aims to solve the problem of bubbles in the eluate.

The utility model provides a pressure stabilizer, includes infusion mechanism, pressure measurement mechanism and exhaust mechanism, infusion mechanism is used for carrying the eluent through the degasification, pressure measurement mechanism locates infusion mechanism with between the exhaust mechanism, pressure measurement mechanism is used for detecting the pressure of eluent.

In one embodiment, the infusion device further comprises a buffer structure, and the buffer structure is arranged between the infusion mechanism and the pressure detection mechanism.

In one embodiment, the cushioning structure is a damper.

In one embodiment, the buffer structure further comprises a buffer pipeline wound to form the buffer structure.

In one embodiment, the infusion mechanism comprises at least two plunger pumps in series.

In one embodiment, the infusion mechanism comprises at least two plunger pumps connected in parallel.

In one embodiment, the infusion device further comprises a connecting mechanism, a first pipeline and a second pipeline, wherein one end of the first pipeline is connected with the infusion mechanism, the other end of the first pipeline is connected with the connecting mechanism, one end of the second pipeline is connected with the connecting mechanism, and the other end of the second pipeline is connected with the exhaust mechanism.

In one embodiment, the maximum inner diameter of the first conduit is less than the minimum inner diameter of the second conduit.

A glycosylated hemoglobin analysis system comprises a hemolysis device, a liquid storage device, a degassing device, the pressure stabilizing device, an elution device and a detection device, wherein the liquid storage device, the degassing device, a transfusion mechanism, the elution device and the detection device are sequentially connected, and the hemolysis device is connected with the elution device.

In one embodiment, the device further comprises a waste liquid collecting device, and the waste liquid collecting device is connected with the detection device.

In one embodiment, the hemolysis device is provided with a hemolysis bin and a waste liquid bin which are separated, and the waste liquid bin is communicated with the exhaust mechanism and the waste liquid collecting device.

In one embodiment, the device further comprises a reversing valve, wherein the reversing valve is connected with the hemolysis device and the elution device and is connected with the transfusion mechanism and the elution device.

A multifunctional all-in-one machine at least comprises a blood routine detection system and the glycosylated hemoglobin analysis system.

Implement the utility model discloses an embodiment will have following beneficial effect at least:

the pressure detection mechanism is used for detecting the pressure of the eluent, when the pressure of the eluent detected by the pressure detection mechanism is lower than a pressure threshold value, the eluent contains air bubbles, air is exhausted through the exhaust mechanism, the air bubbles in the eluent can be eliminated, and the effect of adjusting the pressure of the eluent can be achieved. The pressure stabilizing device is applied to a glycosylated hemoglobin analysis system and a multifunctional all-in-one machine, so that the measurement precision of the glycosylated hemoglobin can be improved.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Wherein:

FIG. 1 is a block diagram of a glycated hemoglobin analysis system according to an embodiment;

FIG. 2 is a schematic view showing a first embodiment of a pressure stabilizer in the glycated hemoglobin analysis system shown in FIG. 1;

FIG. 3 is a schematic view showing a second embodiment of a pressure stabilizer in the glycated hemoglobin analysis system shown in FIG. 1;

FIG. 4 is a partial schematic view of a third embodiment of a pressure stabilizer in the glycated hemoglobin analysis system shown in FIG. 1;

FIG. 5 is a partial schematic view of a fourth embodiment of a pressure stabilizer in the glycated hemoglobin analysis system shown in FIG. 1;

FIG. 6 is a schematic view of a hemolysis apparatus in the glycated hemoglobin analysis system shown in FIG. 1;

FIG. 7 is a block diagram of the multifunction kiosk of an embodiment;

FIG. 8 is a block diagram of a blood routine testing system in the multifunctional all-in-one machine of FIG. 7.

Detailed Description

In order to facilitate understanding of the present invention, the present invention will be described more fully hereinafter with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. The invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

As shown in FIG. 1, a glycated hemoglobin analysis system according to one embodiment includes a hemolyzing device 10, a reservoir device 20, a degassing device 30, a pressure stabilizing device 40, an elution device 50, and a detection device 60.

The hemolysis apparatus 10 is a structure for mixing a whole blood sample, which may be venous blood or peripheral blood, with a hemolytic agent in a certain ratio and performing hemolysis.

The liquid storage device 20 is used for storing eluent, in the present embodiment, the liquid storage device 20 includes at least two liquid storage tanks, and the concentration of the eluent stored in each liquid storage tank is different, so as to perform gradient elution. The eluent in each liquid storage tank can be prepared in advance and can directly enter the elution device 50 after degassing, or a mixing device can be additionally arranged at the downstream of the liquid storage device 20 and proportionally mixes the eluents in different liquid storage tanks to generate new eluent meeting the concentration requirement.

The degassing device 30 is used for degassing micro-bubbles existing in the eluent, in the embodiment, the degassing device 30 and the liquid storage tanks are arranged in a one-to-one correspondence manner, that is, each liquid storage tank is connected with one degassing device 30, so as to avoid mixing of different eluents to influence the elution effect and further influence the final detection result. In other embodiments, multiple reservoirs may share a single degasser 30.

The pressure stabilizer 40 includes a liquid feeding mechanism 100, a pressure detecting mechanism 200 and an exhaust mechanism 300, the liquid feeding mechanism 100 is connected to the degasser 30 and is used for conveying degassed eluent, on one hand, the liquid feeding mechanism 100 is used for conveying eluent to the pressure detecting mechanism 200 and the exhaust mechanism 300, on the other hand, the liquid feeding mechanism 100 is used for conveying eluent to the elution device 50. The infusion mechanism 100 may be powered by a plunger pump or the like.

The pressure detection means 200 is provided between the liquid feeding means 100 and the air discharging means 300, and detects the pressure of the eluent. The pressure detection mechanism 200 has a preset pressure threshold, and when the pressure value of the eluent detected by the pressure detection mechanism 200 is less than or equal to the pressure threshold, it indicates that bubbles still exist in the eluent, and the air exhaust mechanism 300 is required to perform air exhaust operation. When the pressure value of the eluent detected by the pressure detection mechanism 200 is greater than the pressure threshold value, the air exhaust mechanism 300 is always in the closed state. The cooperation of the pressure detection mechanism 200 and the exhaust mechanism 300 can eliminate air bubbles in the eluent, and can also play a role in adjusting the pressure of the eluent, thereby improving the final detection precision. The pressure detection mechanism 200 may be a sputtering film pressure sensor or the like.

The exhaust mechanism 300 may be a manual exhaust mechanism, and after the pressure stabilizing device 40 enters the working state and the pressure of the infusion mechanism 100 is stabilized, when the pressure value of the eluent detected by the pressure detection mechanism 200 is less than or equal to the pressure threshold value, the controller may send a prompt signal according to the detection result of the pressure detection mechanism 200 to prompt the user to open the exhaust mechanism 300 for exhausting. The exhaust mechanism 300 may also be an automatic exhaust mechanism, and when the pressure value of the eluent detected by the pressure detection mechanism 200 is less than or equal to the pressure threshold, the controller controls the exhaust mechanism 300 according to the detection result of the pressure detection mechanism 200, so that the exhaust mechanism 300 is opened.

In the present embodiment, the pressure threshold is 4MPa, and it is understood that the pressure threshold may be different depending on the application scenario of the pressure stabilizer 40.

Referring to fig. 1, 2 and 3, the pressure stabilizer 40 further includes a connecting mechanism 500, a first pipeline 600 and a second pipeline 700, one end of the first pipeline 600 is connected to the infusion mechanism 100, the other end of the first pipeline 600 is connected to the connecting mechanism 500, one end of the second pipeline 700 is connected to the connecting mechanism 500, and the other end of the second pipeline 700 is connected to the exhaust mechanism 300.

In the present embodiment, the connection mechanism 500 is a three-way joint, and the liquid feeding mechanism 100 feeds the eluent to the elution device 50 through the connection mechanism 500, and feeds the eluent to the pressure detection mechanism 200 and the air discharge mechanism 300 through the connection mechanism 500. In some embodiments, the connection mechanism 500 may be omitted, and in this case, fluid outlets corresponding to the elution unit 50 and the air exhaust mechanism 300 may be provided on the infusion mechanism 100.

The first pipe 600 may be a continuous pipe or a plurality of pipes connected in sequence. Similarly, the second pipe 700 may be a continuous pipe or may include a plurality of pipes.

The inner diameter of the first conduit 600 is smaller than the inner diameter of the second conduit 700, it being understood that the maximum inner diameter of the first conduit 600 is smaller than the minimum inner diameter of the second conduit 700. In the present embodiment, the maximum inner diameter of the first conduit 600 is 0.17mm to 0.50mm, and preferably, the maximum inner diameter of the first conduit 600 is 0.254 mm. The minimum inner diameter of the second pipe 700 is preferably 0.75 mm. The first and

second conduits

600 and 700 are thus arranged to obtain a desired flow rate and pressure.

In this embodiment, the pressure stabilizer 40 further includes a buffer structure disposed between the infusion mechanism 100 and the pressure detection mechanism 200, and the buffer structure is used for buffering the fluctuation of the flow rate and the pressure of the eluent flowing out from the infusion mechanism 100, so that the detection result is more accurate.

In an embodiment, referring to fig. 2 in combination with fig. 1, the buffering structure is a damper 410, and in this embodiment, the damper 410 is a diaphragm type damper including a built-in diaphragm, and when the output pressure of the infusion mechanism 100 is higher during the process that the eluent flows from the inlet of the damper 410 to the outlet of the damper 410 along the diaphragm, the diaphragm deforms more, and the response value of the damper 410 is higher, that is, the damping effect of the damper 410 is higher. The smaller the output pressure of the infusion mechanism 100, the smaller the diaphragm deflection and the smaller the response of the damper 410, i.e., the lower the damping effect of the damper 410. When the output pressure of the infusion mechanism 100 fluctuates, the diaphragm is also deformed within a range so as to stabilize the flow rate and pressure of the eluent.

In this embodiment, the second pipe 700 at least includes a first connecting section 710 and a second connecting section 720, two ends of the first connecting section 710 are respectively connected to the connecting mechanism 500 and the damper 410, and two ends of the second connecting section 720 are respectively connected to the damper 410 and the exhaust mechanism 300. In some embodiments, the pressure detection mechanism 200 may be a clip-type structure that clips onto the second connecting section 720. Of course, if the pressure detection mechanism 200 is a pipe connection structure, two ends of the second connection section 720 are respectively connected to the damper 410 and the pressure detection mechanism 200, the second pipeline 700 further includes at least a third connection section 730, and two ends of the third connection section 730 are respectively connected to the pressure detection mechanism 200 and the exhaust mechanism 300.

In an embodiment, referring to fig. 3 in combination with fig. 1, the voltage stabilizer 40 further includes a buffer pipe, the buffer pipe is wound to form a buffer structure 420, and the buffer structure 420 is wound at least one turn. This buffer structure 420 can reduce the velocity of flow of eluant and alleviate the fluctuation of pressure, improves the accuracy of testing result, and this buffer structure 420 simple structure, and the cost is lower.

In this embodiment, if the pressure detecting mechanism 200 is a clamping structure, the buffer pipe may correspond to the second pipe 700. If the pressure detection mechanism 200 has a pipe connection structure, the buffer pipe constitutes a part of the second pipe 700.

In addition to the buffer structure, the flow rate and pressure of the eluent can be adjusted by providing a plurality of plunger pumps.

Specifically, in an embodiment, referring to fig. 4 in combination with the infusion mechanism 100 of fig. 1, the infusion mechanism includes two plunger pumps connected in series, namely a first plunger pump 110 and a second plunger pump 120, the first plunger pump 110 is connected between the degassing device 30 and the second plunger pump 120, the first plunger pump 110 and the second plunger pump 120 operate in opposite modes, when the first plunger pump 110 performs an action of pumping the eluent, the second plunger pump 120 performs an action of discharging the eluent, and when the first plunger pump 110 performs an action of discharging the eluent, the second plunger pump 120 performs an action of pumping the eluent. By controlling the pumping or discharging frequency of the first plunger pump 110 and the second plunger pump 120, the influence on the flow rate and pressure of the output eluent when the two actions of pumping the eluent or discharging the eluent are switched can be eliminated. In other embodiments, the number of the plunger pumps connected in series may be more than two, or three, four or more, which are not illustrated here.

In one embodiment, referring to fig. 5, the infusion mechanism 100 includes two plunger pumps connected in parallel, namely a third plunger pump 130 and a fourth plunger pump 140, and the operation modes of the third plunger pump 130 and the fourth plunger pump 140 may be the same or opposite, and the influence on the flow rate and the pressure of the output eluent when the two actions of pumping the eluent or discharging the eluent are switched can be eliminated by controlling the pumping or discharging frequencies of the third plunger pump 130 and the fourth plunger pump 140. Of course, in other embodiments, the number of plunger pumps connected in parallel may be three or more.

It should be noted that the embodiment shown in fig. 4 and 5 can be used in combination with damper 410 in the embodiment shown in fig. 2, or in combination with cushioning structure 420 in the embodiment shown in fig. 3.

The elution device 50 is filled with cation exchange resin for adsorbing hemoglobin, and when eluents with different concentrations flow through the elution device 50, different sub-components with weak to strong binding strength with the cation exchange resin can be sequentially eluted and brought into the detection device 60 together, and colorimetric analysis is performed by the detection device 60.

In this embodiment, the glycated hemoglobin analysis system further includes a direction change valve 70, and the direction change valve 70 connects the hemolysis device 10 and the elution device 50, and connects the elution device 50 and the infusion mechanism 100. The direction valve 70 may be a five-way valve or a six-way valve with a quantitative column, and the direction valve 70 is mainly used to switch the on/off of each pipeline, for example, during the elution process, the direction valve 70 needs to ensure the communication between the infusion mechanism 100 and the elution device 50, and the hemolysis device 10 is disconnected from the infusion mechanism 100.

Glycated haemoglobin analytic system still includes waste liquid collection device 80, and waste liquid collection device 80 is connected with detection device 60, and the eluent that mixes the hemoglobin subcomponent can directly get into in waste liquid collection device 80 after detecting.

It should be noted that the hemolysis apparatus 10 of the present embodiment has a function of collecting waste liquid in addition to the function of hemolysis.

Specifically, as shown in fig. 1 and 6, the hemolysis device 10 is provided with a hemolysis chamber 12 and a waste liquid chamber 14 which are separated, and the waste liquid chamber 14 is communicated with an exhaust mechanism 300 and a waste liquid collecting device 80. When the exhaust mechanism 300 exhausts the gas, a small amount of the eluent is also exhausted, and the part of the eluent enters the waste liquid bin 14, and when the amount of the waste liquid in the waste liquid bin 14 reaches a certain amount, the waste liquid enters the waste liquid collecting device 80.

The hemolysis apparatus 10 as a transit waste liquid collecting structure can reduce the length of the piping from the air discharging mechanism 300 to the waste liquid collecting apparatus 80, thereby reducing the cost of the glycated hemoglobin analyzing system. Meanwhile, the structure layout of the glycosylated hemoglobin analysis system is more facilitated. Of course, in other embodiments, the exhaust mechanism 300 may be directly connected to the waste liquid collecting device 80.

For the glycated hemoglobin analysis system of the present embodiment, the present invention can be applied not only to a glycated hemoglobin analyzer for glycated hemoglobin measurement, but also to a multifunctional integrated machine, and the multifunctional integrated machine provided in fig. 7 includes not only the glycated hemoglobin analysis system 1, but also a blood routine measurement system 2, i.e., the multifunctional integrated machine can be used for both glycated analysis of hemoglobin and blood routine measurement, thereby greatly improving the measurement efficiency of multiple items.

Moreover, the multifunctional all-in-one machine further comprises a collection module 3 and a distribution module 4, wherein the collection module 3 is used for collecting blood samples, and the distribution module 4 is used for distributing the blood samples collected by the collection module 3 to the glycosylated hemoglobin analysis system 1 and the blood routine detection system 2 in proportion. To medical personnel, only need gather patient's blood sample once, improved collection efficiency, to medical institution, can reduce the test tube of using storage blood sample, greatly reduced the consumptive material cost.

It is understood that the multifunctional integrated machine can be used for detecting other items, such as blood coagulation detection system, etc., without being limited to blood routine detection.

In this embodiment, referring to fig. 7 and 8, the blood routine test system 2 includes: the device comprises a reagent providing device 201, a blood sample reaction device 202 and a blood sample detection device 203, wherein the reagent providing device 201 is used for providing reagents used for analyzing a blood sample to be detected; the blood sample reaction device 202 is configured to receive the blood sample to be tested provided by the distribution module 4 and the reagent provided by the reagent providing device 201, and mix and react the blood sample to be tested to obtain a reacted blood sample to be tested; the blood sample detection device 203 is configured to receive the reacted blood sample to be detected, and perform blood cell analysis on the reacted blood sample to be detected to obtain blood routine parameters of the blood sample to be detected. Further, the blood routine detecting system 2 may detect a blood sample to be detected and output a three-classification detection result or a five-classification detection result, and which specific output result may be determined by selecting a corresponding configuration or a detection mode of the blood routine detecting system 2 according to a user requirement, which is not specifically limited herein. Further, to meet different requirements, the detection methodology adopted by the blood routine detection system 2 can be determined according to the detection requirements, and is not particularly limited herein.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the claims. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. The pressure stabilizing device is characterized by comprising an infusion mechanism, a pressure detection mechanism and an exhaust mechanism, wherein the infusion mechanism is used for conveying degassed eluent, the pressure detection mechanism is arranged between the infusion mechanism and the exhaust mechanism, and the pressure detection mechanism is used for detecting the pressure of the eluent.

2. The pressure stabilization device of claim 1, further comprising a buffer structure disposed between the infusion mechanism and the pressure detection mechanism.

3. The voltage stabilizer apparatus according to claim 2, wherein the buffer structure is a damper.

4. The pressure stabilizer according to claim 2, characterized in that further comprises a buffer pipe wound to form the buffer structure.

5. The pressure stabilization device of claim 1, wherein the infusion mechanism comprises at least two plunger pumps in series.

6. The pressure stabilization device of claim 1, wherein the infusion mechanism comprises at least two plunger pumps in parallel.

7. The pressure stabilizing device according to any one of claims 1 to 6, further comprising a connection mechanism, a first pipe and a second pipe, wherein one end of the first pipe is connected to the liquid feeding mechanism, the other end of the first pipe is connected to the connection mechanism, one end of the second pipe is connected to the connection mechanism, and the other end of the second pipe is connected to the air discharging mechanism.

8. The pressure stabilizer according to claim 7, characterized in that the maximum inner diameter of the first conduit is smaller than the minimum inner diameter of the second conduit.

9. A glycated hemoglobin analysis system comprising a hemolysis device, a liquid storage device, a degassing device, the pressure stabilizing device according to any one of claims 1 to 8, an elution device, and a detection device, wherein the liquid storage device, the degassing device, the transfusion mechanism, the elution device, and the detection device are connected in series, and the hemolysis device is connected to the elution device.

10. The glycated hemoglobin analysis system as set forth in claim 9, further comprising a waste liquid collecting device connected to the measuring device.

11. The glycated hemoglobin analysis system as set forth in claim 10, wherein the hemolysis device is provided with a separate hemolysis compartment and a waste liquid compartment, the waste liquid compartment communicating the exhaust mechanism and the waste liquid collection device.

12. The glycated hemoglobin analysis system of claim 9, further comprising a diverter valve connecting the hemolysis device and the elution device and connecting the infusion mechanism and the elution device.

13. A multifunctional integrated machine comprising at least a blood routine test system and the glycated hemoglobin analysis system according to any one of claims 9 to 12.