CN220872420U

CN220872420U - Serum analyzer

Info

Publication number: CN220872420U
Application number: CN202322376151.4U
Authority: CN
Inventors: 王超智; 张云海; 周毅; 周立; 栗琳; 丁亮
Original assignee: Hunan Haosi Biotechnology Co ltd; Jiangsu Hao Si Biological Technology Co ltd; Suzhou Haosi Medical Laboratory Co ltd; Beijing Haosi Biotechnology Co ltd
Current assignee: Hunan Haosi Biotechnology Co ltd; Jiangsu Hao Si Biological Technology Co ltd; Suzhou Haosi Medical Laboratory Co ltd; Beijing Haosi Biotechnology Co ltd
Priority date: 2023-09-01
Filing date: 2023-09-01
Publication date: 2024-04-30
Anticipated expiration: 2033-09-01

Abstract

The utility model relates to the technical field of medical analysis equipment, in particular to a serum analyzer, which comprises a sample injection assembly, a first adapter, a two-dimensional extraction column, a two-dimensional chromatographic column, a first pump body and a two-dimensional detector, wherein the first adapter is suitable for being switched between a first position and a second position, the sample injection assembly is communicated with the two-dimensional extraction column, the first pump body, the two-dimensional extraction column and the two-dimensional chromatographic column are sequentially communicated with the two-dimensional detector, the two-dimensional detector is a 265 nanometer fixed wavelength ultraviolet detector, and the intensity of the ultraviolet detector is higher than that of a conventional variable wavelength ultraviolet detector by arranging the 265 nanometer fixed wavelength ultraviolet detector behind the two-dimensional chromatographic column, so that the response of 25-hydroxy vitamin D is increased, and the detection effect of the serum analyzer on the 25-hydroxy vitamin D is further improved.

Description

Serum analyzer

Technical Field

The utility model relates to the technical field of medical analysis equipment, in particular to a serum analyzer.

Background

High performance liquid chromatography is also called "high pressure liquid chromatography", "high performance liquid chromatography", "high resolution liquid chromatography", "modern column chromatography", etc. High performance liquid chromatography is an important branch of chromatography, liquid is taken as a mobile phase, a high pressure transfusion system is adopted, a single solvent with different polarities or a mixed solvent with different proportions, buffer solution and other mobile phases are pumped into a chromatographic column filled with a stationary phase, and after components in the column are separated, the components enter a detector for detection, so that analysis of a sample is realized. The method has become an important application of separation analysis technology in the subject fields of chemistry, medicine, industry, agriculture, commercial inspection, legal inspection and the like.

When the vitamin A, E and the 25-hydroxy vitamin D in serum are measured, compared with an enzyme-linked immunosorbent method and a chemiluminescence method, the high performance liquid chromatography has the advantages of high sensitivity, good repeatability and capability of distinguishing different derivatives, but the sensitivity is still not high enough when the 25-hydroxy vitamin D in serum is measured.

Disclosure of utility model

The utility model provides a serum analyzer, which is used for solving the defects that the content of 25-hydroxy vitamin D in serum is low and difficult to detect in the prior art and realizing the improvement of the detection effect of the serum analyzer on the 25-hydroxy vitamin D.

The utility model provides a serum analyzer, which comprises a sample injection assembly, a first adapter, a two-dimensional extraction column, a two-dimensional chromatographic column, a first pump body and a two-dimensional detector, wherein the first adapter is suitable for being switched between a first position and a second position, the sample injection assembly is communicated with the two-dimensional extraction column in the first position, the first pump body, the two-dimensional extraction column, the two-dimensional chromatographic column and the two-dimensional detector are sequentially communicated in the second position, and the two-dimensional detector is a 265 nanometer fixed wavelength ultraviolet detector.

According to one embodiment of the present utility model, a flow cell with an optical path of 50 mm is disposed in the two-dimensional detector.

According to one embodiment of the present utility model, the sample injection assembly includes a sample injection member, a second adapter, a first one-dimensional extraction column, a one-dimensional chromatography column, and a second pump body, wherein the second adapter is adapted to switch between a third position in which the sample injection member is in communication with the first one-dimensional extraction column, and a fourth position in which the second pump body, the first one-dimensional extraction column, the one-dimensional chromatography column, and the first adapter are in sequential communication.

According to one embodiment of the present utility model, the one-dimensional chromatographic column further comprises a one-dimensional detector, wherein the first adapter is at the first position, the one-dimensional chromatographic column, the two-dimensional extraction column and the one-dimensional detector are sequentially communicated, and the first adapter is at the second position, and the one-dimensional chromatographic column is communicated with the one-dimensional detector.

According to one embodiment of the present utility model, the sample injection assembly further includes a second one-dimensional extraction column, the second adapter is at the third position, the second pump body, the second one-dimensional extraction column and the one-dimensional chromatographic column are sequentially communicated, the second adapter is at the fourth position, and the sample injection member is communicated with the second one-dimensional extraction column.

According to one embodiment of the present utility model, the sample injection component includes an SPE pump and an autosampler, and the SPE pump, the autosampler and the second adapter are sequentially communicated.

According to one embodiment of the present utility model, the first adapter is a two-position six-way valve.

According to one embodiment of the present utility model, the second adapter is a two-position, ten-way valve.

According to one embodiment of the utility model, the second adapter is in the third position and the first adapter is in the second position in case of sample introduction and extraction; in the case of eluting and detecting vitamin a, the second adapter is in the fourth position and the first adapter is in the second position; in the case of capturing 25-hydroxyvitamin D, the second adapter is in the fourth position and the first adapter is in the first position; in the case of vitamin E detection and 25-hydroxyvitamin D detection, the second adapter is in the fourth position and the first adapter is in the second position.

According to the serum analyzer provided by the utility model, the first adapter is positioned at the first position, the sample injection assembly is communicated with the two-dimensional extraction column, the sample injection assembly is suitable for sending an external sample into the two-dimensional extraction column, the two-dimensional extraction column is in a sample loading state, the sample is extracted, separated and concentrated in the two-dimensional extraction column, and 25-hydroxy vitamin D is enriched in the two-dimensional extraction column, after the two-dimensional extraction column is enriched, the first adapter is switched to the second position, at the moment, the first pump body, the two-dimensional extraction column and the two-dimensional chromatographic column are sequentially communicated with the two-dimensional detector, the first pump body is suitable for providing a first mobile phase, 25-hydroxy vitamin D stored in the two-dimensional extraction column is eluted when the first mobile phase flows through the two-dimensional extraction column, the two-dimensional extraction column is in an eluting state, the first mobile phase mixed with the 25-hydroxy vitamin D is flushed to the two-dimensional chromatographic column along a pipeline, the separated liquid is continuously fed into the two-dimensional detector, and the two-dimensional detector is detected, and the two-dimensional detector is 265 nm fixed wavelength ultraviolet detector.

The ultraviolet detector with 265 nanometers and fixed wavelength is arranged behind the two-dimensional chromatographic column, and the light intensity of the ultraviolet detector is higher than that of a conventional ultraviolet detector with variable wavelength, so that the response of 25-hydroxy vitamin D is ensured to be increased, and the detection effect of the serum analyzer on 25-hydroxy vitamin D is further improved.

When the first adapter is positioned at the first position, the first pump body is sequentially communicated with the two-dimensional chromatographic column and the two-dimensional detector. Namely, when the first adapter is in the first position in the working process of the serum analyzer, the two-dimensional extraction column is in a loading state, only a first mobile phase flows in the first pump body, the two-dimensional chromatographic column and the two-dimensional detector, so that the isolation from the outside is ensured, the interference of impurities is avoided, and the measured mobile phase parameters in the two-dimensional detector can be compared with the measured parameters of the mobile phase mixed with 25-hydroxy vitamin D, which are measured when the first adapter is in the second position, so that the interference caused by partial mobile phase is eliminated, and the accuracy of a detection result is improved.

Drawings

In order to more clearly illustrate the utility model or the technical solutions of the prior art, the following description will briefly explain the drawings used in the embodiments or the description of the prior art, and it is obvious that the drawings in the following description are some embodiments of the utility model, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of a serum analyzer provided by the present utility model;

FIG. 2 is one of the schematic connection diagrams of the serum analyzer provided by the present utility model;

FIG. 3 is a second schematic diagram of the connection of the serum analyzer provided by the present utility model;

FIG. 4 is a third schematic illustration of the connection of the serum analyzer provided by the present utility model.

Reference numerals:

100. A sample injection assembly; 110. a sample introduction part; 111. SPE pump; 112. an autosampler; 120. a second adapter; 130. a first one-dimensional extraction column; 140. a one-dimensional chromatographic column; 150. a second pump body; 160. a second one-dimensional extraction column; 170. a waste liquid tank;

200. A first adapter; 300. a two-dimensional extraction column; 400. a two-dimensional chromatographic column; 500. a first pump body; 600. a two-dimensional detector; 700. a one-dimensional detector; 800. column incubator.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the present utility model more apparent, the technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some embodiments of the present utility model, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1, the present utility model provides a serum analyzer, which includes a sample injection assembly 100, a first adapter 200, a two-dimensional extraction column 300, a two-dimensional chromatographic column 400, a first pump body 500, and a two-dimensional detector 600, wherein the first adapter 200 is adapted to switch between a first position, in which the sample injection assembly 100 is in communication with the two-dimensional extraction column 300, and a second position, in which the first pump body 500, the two-dimensional extraction column 300, the two-dimensional chromatographic column 400 are in communication with the two-dimensional detector 600, and the two-dimensional detector 600 is a 265 nm fixed wavelength ultraviolet detector.

In the serum analyzer of the embodiment of the present utility model, as shown in fig. 4, the first adapter 200 is in the first position, at this time, the sample injection assembly 100 is communicated with the two-dimensional extraction column 300, the sample injection assembly 100 is adapted to send an external sample into the two-dimensional extraction column 300, the two-dimensional extraction column 300 is in a loading state, the sample is extracted, separated and concentrated in the two-dimensional extraction column 300, and the 25-hydroxy vitamin D is enriched, after the two-dimensional extraction column 300 is enriched, the first adapter 200 is switched to the second position, as shown in fig. 3, at this time, the first pump body 500, the two-dimensional extraction column 300 and the two-dimensional chromatography column 400 are sequentially communicated with the two-dimensional detector 600, the first pump body 500 is adapted to provide a first mobile phase, the first mobile phase elutes 25-hydroxy vitamin D stored in the two-dimensional extraction column 300 when flowing through the two-dimensional extraction column 300, the two-dimensional extraction column 300 is in an eluting state, the first mobile phase mixed with the 25-hydroxy vitamin D is washed to the two-dimensional chromatography column 400 along a pipeline, and the separated liquid is introduced into the two-dimensional detector 600 to complete detection, wherein the two-dimensional detector 600 is a nano fixed wavelength ultraviolet detector 265.

It should be noted that, because the content of 25-hydroxyvitamin D in serum is low, although the conventional liquid chromatography has the advantages of high sensitivity, good repeatability and capability of distinguishing different derivatives compared with the enzyme-linked immunosorbent assay and the chemiluminescence method, the sensitivity of the conventional liquid chromatograph is still not high enough when the 25-hydroxyvitamin D is measured, and the serum analyzer in the embodiment of the utility model ensures that the response of the 25-hydroxyvitamin D is increased by configuring the 265 nanometer fixed wavelength ultraviolet detector behind the two-dimensional chromatographic column 400, and the light intensity of the serum analyzer is higher than that of the conventional variable wavelength ultraviolet detector, thereby improving the detection effect of the serum analyzer on the 25-hydroxyvitamin D.

In this embodiment, as shown in fig. 4, when the first adapter 200 is in the first position, the first pump body 500 is in communication with the two-dimensional chromatographic column 400 and the two-dimensional detector 600 in sequence. That is, when the first adapter 200 is at the first position during the operation of the serum analyzer, the two-dimensional extraction column 300 is in the loading state, and only the first mobile phase flows in the first pump body 500, the two-dimensional chromatographic column 400 and the two-dimensional detector 600, so as to ensure isolation from the outside, avoid interference of impurities, and the measured mobile phase parameter in the two-dimensional detector 600 can be compared with the measured parameter of the mobile phase mixed with 25-hydroxy vitamin D measured when the first adapter 200 is at the second position, so that interference caused by partial mobile phase is discharged, and accuracy of a detection result is improved.

In this embodiment, the serum analyzer further comprises a column incubator 800 adapted to heat and insulate the two-dimensional chromatographic column 400 and the two-dimensional extraction column 300, and to provide a suitable stable temperature for sample separation, extraction, and the like.

In this embodiment, the two-dimensional detector 600 is a 265 nm fixed wavelength ultraviolet detector, detects 25-hydroxyvitamin D, and mainly detects 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2, and in other embodiments, the fixed wavelength ultraviolet detector specification can be adjusted according to different detection requirements.

In this embodiment, the first mobile phase is a mixed solution of acetonitrile and methanol, and in other embodiments, mobile phases with different material compositions may be replaced according to the detection sample.

According to one embodiment of the present utility model, a flow cell with an optical path of 50 mm is provided in the two-dimensional detector 600. In this embodiment, by arranging the flow cell with an optical path of 50 mm in the two-dimensional detector 600, compared with the conventional detector with a flow cell of 10 mm, the absorption rate of the two-dimensional detector 600 is improved, so as to obtain a smaller sample detection concentration, i.e. improve the detection sensitivity of the two-dimensional detector 600, and ensure the response increase of 25-hydroxy vitamin D.

According to one embodiment of the present utility model, the sample assembly 100 comprises a sample member 110, a second adapter 120, a first one-dimensional extraction column 130, a one-dimensional chromatography column 140, and a second pump body 150, wherein the second adapter 120 is adapted to switch between a third position, wherein the sample member 110 is in communication with the first one-dimensional extraction column 130, and a fourth position, wherein the second pump body 150, the first one-dimensional extraction column 130, and the one-dimensional chromatography column 140 are in communication with the first adapter 200.

In this embodiment, as shown in fig. 2, the second adaptor 120 is located at the third position, the sample injection component 110 is communicated with the first one-dimensional extraction column 130, the sample injection component 110 is adapted to send an external sample into the first one-dimensional extraction column 130, the first one-dimensional extraction column 130 is in a sample loading state, the sample in the first one-dimensional extraction column 130 is extracted, separated and concentrated, the vitamin a, vitamin E and 25-hydroxyvitamin D in the sample are enriched in the first one-dimensional extraction column 130, after the enrichment of the first one-dimensional extraction column 130 is completed, the second adaptor 120 is switched to the fourth position, as shown in fig. 3, at this time, the second pump body 150, the first one-dimensional extraction column 130 and the one-dimensional chromatographic column 140 are sequentially communicated with the first adaptor 200, the second pump body 150 is adapted to provide a second mobile phase, the second mobile phase elutes the vitamin a, vitamin E and 25-hydroxyvitamin D stored in the first one-dimensional extraction column 130 when the second mobile phase flows through the second one-dimensional extraction column 130, the first one-dimensional extraction column 130 is in an eluting state, the vitamin a, vitamin E and 25-hydroxyvitamin D are mixed in the vitamin a, vitamin a and the vitamin D is separated and 25-hydroxyvitamin D are sequentially eluted along the second mobile phase and the second mobile phase is separated and the vitamin D-vitamin D is separated in the second mobile phase and 25-hydroxyvitamin D is sequentially.

In the prior art, two-dimensional liquid chromatography is an important supplement to conventional liquid chromatography techniques (liquid chromatography including one-dimensional isocratic and gradient elution). Two-dimensional liquid chromatography, which is the continuous transfer of the effluent of one-dimensional chromatography column 140 to two-dimensional chromatography column 400, can be divided into full liquid chromatography and center-cut chromatography; in contrast, the center cut chromatography selectively transfers the effluent of the one-dimensional chromatography column 140 to the two-dimensional chromatography column 400. In centre cut chromatography, the target is one or more chromatographic peaks, and a fraction of the corresponding chromatographic peak is collected and injected into a second chromatographic column. The whole chromatographic peak of the individual fractions may be collected, or the fractions near the front, middle or tail of the chromatographic peak may be selected as desired. Centre cut chromatography is very useful for separating samples that are less complex and contain compounds with very similar retention properties. Compared with full two-dimensional liquid chromatography, the system construction and method setting of the mode are generally simpler, and the use cost is lower. This mode allows sampling of the plurality of chromatographic peaks in the first dimension according to the length of the analysis time in the second dimension and sequential analysis in the second dimension.

In this embodiment, by arranging the first one-dimensional extraction column 130, the one-dimensional chromatographic column 140, the two-dimensional extraction column 300 and the two-dimensional chromatographic column 400, that is, applying the center-cut chromatography to the serum analyzer, the separation of the one-dimensional chromatographic column 140 achieves the primary purification of 25-hydroxyvitamin D, which is beneficial to improving the accuracy of the detection result of the two-dimensional detector 600; and the effluent of the one-dimensional chromatographic column 140 is selectively transferred to the two-dimensional extraction column 300 through the center-cut chromatography, so that the detection limit of the 25-hydroxy vitamin D in the two-dimensional detector 600 is improved, and the detection effect of the serum analyzer on the 25-hydroxy vitamin D is further improved.

In this embodiment, as shown in fig. 2, when the second adapter 120 is in the third position, the second pump body 150, the one-dimensional chromatographic column 140 and the first adapter 200 are sequentially connected. Namely, when the second adapter 120 is at the third position in the working process of the serum analyzer, the first one-dimensional extraction column 130 is in the loading state at this time, and only the second mobile phase flows in the second pump body 150, the one-dimensional chromatographic column 140 and the first adapter 200, so that the isolation from the outside is ensured, and the interference of impurities is avoided.

In this embodiment, the sample assembly 100 further includes a waste tank 170, and in the third position of the second adapter 120, the sample member 110, the first one-dimensional extraction column 130 and the waste tank 170 are sequentially connected, the waste tank 170 is adapted to collect the waste liquid after being extracted after flowing through the first one-dimensional extraction column 130,

In this embodiment, the second mobile phase is a mixed solution of acetonitrile and water, and in other embodiments, mobile phases of different material compositions may be replaced according to the sample to be tested.

According to one embodiment of the present utility model, the serum analyzer further comprises a one-dimensional detector 700, the first adapter 200 is in a first position, the one-dimensional chromatographic column 140, the two-dimensional extraction column 300 and the one-dimensional detector 700 are in communication in sequence, and the first adapter 200 is in a second position, the one-dimensional chromatographic column 140 is in communication with the one-dimensional detector 700.

In this embodiment, as shown in fig. 4, the first adaptor 200 is in a first position, the one-dimensional chromatographic column 140, the two-dimensional extraction column 300 and the one-dimensional detector 700 are sequentially connected, at this time, the sample in the first one-dimensional extraction column 130 has been extracted, separated and concentrated, the vitamin a, the vitamin E and the 25-hydroxy vitamin D in the sample are concentrated in the first one-dimensional extraction column 130, when the second adaptor 120 is switched to a fourth position, as shown in fig. 3, at this time, the second pump body 150, the first one-dimensional extraction column 130, the one-dimensional chromatographic column 140, the first adaptor 200 and the one-dimensional detector 700 are sequentially connected, the second pump body 150 is adapted to provide a second mobile phase, the second mobile phase elutes the vitamin a, the vitamin E and the 25-hydroxy vitamin D stored in the first one-dimensional extraction column 130 when flowing through the first one-dimensional extraction column 130, the first one-dimensional extraction column 130 is in an eluted state, the second mobile phase mixed with the vitamin a, the vitamin E and the 25-hydroxy vitamin D is flushed into the one-dimensional chromatographic column 140 along a pipeline, and the vitamin a, the vitamin E and the 25-hydroxy vitamin E is sequentially separated and detected in the one-dimensional detector 700.

In this embodiment, vitamin a and vitamin D are sequentially separated in the one-dimensional chromatographic column 140, and when detecting vitamin a, the wavelength of the one-dimensional detector 700 is the maximum response wavelength of vitamin a, and when detecting vitamin E, the wavelength of the one-dimensional detector 700 is correspondingly adjusted to be the maximum response wavelength of vitamin E.

In this embodiment, by arranging the one-dimensional detector 700, the detection of vitamin a and vitamin E during the separation of the one-dimensional chromatographic column 140 is realized, and the further separation and detection of 25-hydroxy vitamin D in the two-dimensional chromatographic column 400 are realized, so that the detection process of the whole serum analyzer only needs one sample injection, the determination of vitamin a, vitamin E and 25-hydroxy vitamin D in the sample can be completed, the determination time is saved, and the accuracy of serum analysis is improved.

According to an embodiment of the present utility model, the sample injection assembly 100 further includes a second one-dimensional extraction column 160, the second adapter 120 is in the third position, the second pump body 150, the second one-dimensional extraction column 160 and the one-dimensional chromatographic column 140 are sequentially communicated, the second adapter 120 is in the fourth position, and the sample injection member 110 is communicated with the second one-dimensional extraction column 160.

In this embodiment, as shown in fig. 2, when the second adapter 120 is at the third position, the second pump body 150, the second one-dimensional extraction column 160 and the one-dimensional chromatographic column 140 are sequentially communicated, and when the first one-dimensional extraction column 130 is in the loading state for the first time, no sample is in the second one-dimensional extraction column 160, and the second mobile phase flowing out of the second pump body 150 sequentially flows through the second one-dimensional extraction column 160 and the one-dimensional chromatographic column 140, and the second one-dimensional extraction column 160 does not enter the eluting state; referring to fig. 3, after the loading of the first one-dimensional extraction column 130 is completed, the second adaptor 120 is switched to the fourth position, at this time, the first extraction column enters an elution state, and the sample injection part 110 is communicated with the second one-dimensional extraction column 160, the sample injection part 110 is adapted to send an external sample into the second one-dimensional extraction column 160, the second one-dimensional extraction column 160 is in a loading state, the sample is extracted, separated and concentrated in the second one-dimensional extraction column 160, and the vitamin a, vitamin E and 25-hydroxyvitamin D in the sample are enriched in the second one-dimensional extraction column 160.

That is, in the rest working processes except when the serum analyzer is first loaded, when the second converter is at the third position, the first one-dimensional extraction column 130 is in a loading state, meanwhile, the second one-dimensional extraction column 160 is in an eluting state, when the second converter is at the fourth position, the first one-dimensional extraction column 130 is in an eluting state, meanwhile, the second one-dimensional extraction column 160 is in a loading state, by arranging the second one-dimensional extraction column 160, after the sample in the first one-dimensional extraction column 130 is measured, the second one-dimensional extraction column 160 which is loaded can be connected in time, and the first one-dimensional extraction column 130 and the second one-dimensional extraction column 160 finish alternate working, so that the analysis time of the serum analyzer is effectively shortened, and the analysis efficiency is improved.

According to one embodiment of the present utility model, the sample injection part 110 includes an SPE (solid phase extraction) pump 111 and an autoinjector 112, and the SPE pump 111, the autoinjector 112 and the second adapter 120 are sequentially connected.

In this embodiment, as shown in fig. 2, the SPE pump 111, the auto-sampler 112 and the second adaptor 120 are sequentially connected, and the SPE pump 111 sends the sample in the auto-sampler 112 to the first one-dimensional extraction column 130 to complete loading.

Solid phase extraction is used to selectively adsorb analytes or interferents in a matrix of a complex sample. In this embodiment, SPE pump 111 and autosampler 112 are used to provide automated solid phase extraction that can be used to enrich analytes, remove matrix components, or reduce the detection limit of the assay. Not only can save a large amount of sample pretreatment time, but also can avoid the unstable recovery rate caused by the complex sample pretreatment process.

According to one embodiment of the present utility model, the first adapter 200 is a two-position six-way valve.

In this embodiment, as shown in fig. 4, the first adapter 200 is a two-position six-way valve, the interfaces of the first adapter 200 and the two-dimensional chromatographic column 400 are the interfaces 1-1, and the other interfaces are the interfaces 1-2 to 1-6 in turn from the interface 1-1 to the interface 1-1.

When the first adaptor 200 is at the first position, as shown in fig. 4, the sample injection assembly 100, the interfaces 1-3, the interfaces 1-2, the two-dimensional extraction column 300, the interfaces 1-5 and the interfaces 1-4 are sequentially communicated; when the first adapter 200 is in the second position, as shown in fig. 3, the first pump body 500, the interfaces 1-6, the interfaces 1-5, the two-dimensional extraction column 300, the interfaces 1-2, the interfaces 1-1, the two-dimensional chromatography column 400 are sequentially communicated with the two-dimensional detector 600.

By arranging the two-position six-way valve, the valve can be dynamically switched between two flow paths without manually cutting off a channel, so that the detection efficiency of the serum analyzer is improved, and meanwhile, the sample is prevented from being polluted by the outside.

According to one embodiment of the present utility model, the second adapter 120 is a two-position, ten-way valve.

In this embodiment, as shown in fig. 2, the second adapter 120 is a two-position ten-way valve, the interfaces of the second adapter 120 and the second pump body 150 are the interfaces 2-1, and the other interfaces are the interfaces 2-2 to 2-10 in turn from the interface 2-1 to the interface 2-10.

When the second adaptor 120 is at the third position, as shown in fig. 2, the sample feeding part 110, the interface 2-8, the interface 2-9, the interface 2-4, the interface 2-5, the first one-dimensional extraction column 130, the interface 2-2 and the interface 2-3 are sequentially communicated; when the second adaptor 120 is in the fourth position, as shown in fig. 3, the second pump body 150, the interface 2-1, the interface 2-2, the first one-dimensional extraction column 130, the interface 2-5, the interface 2-6, and the one-dimensional chromatographic column 140 are sequentially connected to the interface 1-3 of the first adaptor 200.

Through setting up two-position ten-way valve, this valve can be in the dynamic switching between two flow paths, need not the manual disconnection passageway, still avoid the sample to receive external pollution when improving serum analyzer's detection efficiency.

According to one embodiment of the present utility model, in the case of sample introduction and extraction, the second adaptor 120 is in the third position and the first adaptor 200 is in the second position; in the case of eluting and detecting vitamin a, the second adapter 120 is in the fourth position and the first adapter 200 is in the second position; in the case of capturing 25-hydroxyvitamin D, the second adapter 120 is in the fourth position and the first adapter 200 is in the first position; in the case of vitamin E detection and 25-hydroxyvitamin D detection, the second adapter 120 is in the fourth position and the first adapter 200 is in the second position.

In this embodiment, when the serum analyzer starts to work, the serum analyzer enters into a sample injection and extraction state, and referring to fig. 2, the second adapter 120 is at the third position, and the sample injection component 110 is in communication with the first one-dimensional extraction column 130, the sample injection component 110 is adapted to send an external sample into the first one-dimensional extraction column 130, so as to complete sample injection, the first one-dimensional extraction column 130 is in a sample injection state, the sample in the first one-dimensional extraction column 130 is extracted, separated and concentrated, and the vitamin a, vitamin E and 25-hydroxyvitamin D in the sample are enriched in the first one-dimensional extraction column 130; and the first adapter 200 is at the second position, the one-dimensional detector 700 is communicated with the sample injection assembly 100, and the wavelength of the one-dimensional detector 700 is adjusted to the maximum response wavelength of vitamin A.

When the enrichment of the first one-dimensional extraction column 130 is completed, the serum analyzer enters the condition of eluting and detecting vitamin a, at this time, the second adaptor 120 is switched to the fourth position, and as shown in fig. 3, the second pump body 150, the first one-dimensional extraction column 130 and the one-dimensional chromatographic column 140 are sequentially communicated with the first adaptor 200, the second pump body 150 is adapted to provide a second mobile phase, and when the second mobile phase flows through the first one-dimensional extraction column 130, the vitamin a, the vitamin E and the 25-hydroxyvitamin D stored in the first one-dimensional extraction column 130 are eluted, the first one-dimensional extraction column 130 is in an eluting state, the second mobile phase mixed with the vitamin a, the vitamin E and the 25-hydroxyvitamin D is flushed into the one-dimensional chromatographic column 140 along a pipeline, and the vitamin a is separated by the one-dimensional chromatographic column 140 and flows into the one-dimensional detector 700 for detection.

When the detection of the vitamin a in the first one-dimensional extraction column 130 is completed, the serum analyzer enters the condition of capturing 25-hydroxyvitamin D, and as shown in fig. 4, the first adapter 200 is adjusted to the first position at this time, the one-dimensional chromatographic column 140 is communicated with the two-dimensional extraction column 300, the 25-hydroxyvitamin D separated in the one-dimensional chromatographic column 140 enters the two-dimensional extraction column 300, the two-dimensional extraction column 300 is in a loading state, and the 25-hydroxyvitamin D is enriched in the two-dimensional extraction column 300; and the wavelength of the one-dimensional detector 700 is adjusted to the maximum response wavelength of vitamin E.

When the enrichment of the two-dimensional extraction column 300 is completed, the serum analyzer enters the condition of detecting vitamin E and detecting 25-hydroxy vitamin D, and the second adapter 120 is positioned at the fourth position as shown in FIG. 3, at this time, the second pump body 150, the first one-dimensional extraction column 130, the one-dimensional chromatographic column 140, the first adapter 200 and the one-dimensional detector 700 are sequentially communicated, and the vitamin E separated in the one-dimensional chromatographic column 140 is detected in the one-dimensional detector 700; the first adapter 200 is in the second position, at this time, the first pump body 500, the two-dimensional extraction column 300, the two-dimensional chromatography column 400 and the two-dimensional detector 600 are sequentially connected, the first pump body 500 is adapted to provide a first mobile phase, 25-hydroxyvitamin D stored in the two-dimensional extraction column 300 is eluted when the first mobile phase flows through the two-dimensional extraction column 300, the two-dimensional extraction column 300 is in an eluting state, the first mobile phase mixed with 25-hydroxyvitamin D is flushed along a pipeline to the two-dimensional chromatography column 400 for continuous separation, and the separated liquid is introduced into the two-dimensional detector 600 for detection.

The serum analyzer of this example was able to simultaneously determine vitamin a, vitamin E and 25-hydroxyvitamin D in serum; the on-line SPE pump and the center cutting liquid chromatography are used for analyzing the vitamin A, E and the 25-hydroxy vitamin D in the serum, so that the derivatives of the vitamin A, the vitamin E and the 25-hydroxy vitamin D in the serum can be distinguished and accurately quantified, and more accurate reference data is provided for clinical diagnosis.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present utility model, and are not limiting; although the utility model has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present utility model.

Claims

1. The utility model provides a serum analyzer, its characterized in that includes sampling subassembly, first adapter, two-dimensional extraction post, two-dimensional chromatographic column, first pump body and two-dimensional detector, first adapter is suitable for switch between first position and second position the first position, sampling subassembly with two-dimensional extraction post intercommunication the second position, first pump body two-dimensional extraction post two-dimensional chromatographic column with two-dimensional detector communicates in proper order, two-dimensional detector is 265 nanometer fixed wavelength ultraviolet detector.

2. The serum analyzer of claim 1, wherein the two-dimensional detector is provided with a flow cell having an optical path length of 50 mm.

3. The serum analyzer of claim 1, wherein the sample assembly comprises a sample component, a second adapter, a first one-dimensional extraction column, a one-dimensional chromatography column, and a second pump body, the second adapter being adapted to switch between a third position in which the sample component is in communication with the first one-dimensional extraction column, and a fourth position in which the second pump body, the first one-dimensional extraction column, the one-dimensional chromatography column, and the first adapter are in sequential communication.

4. The serum analyzer of claim 3, further comprising a one-dimensional detector, wherein the first adapter is in the first position, the one-dimensional chromatographic column, the two-dimensional extraction column, and the one-dimensional detector are in sequential communication, and wherein the first adapter is in the second position, and wherein the one-dimensional chromatographic column is in communication with the one-dimensional detector.

5. The serum analyzer of claim 4, wherein the sample injection assembly further comprises a second one-dimensional extraction column, the second adapter is in the third position, the second pump body, the second one-dimensional extraction column, and the one-dimensional chromatography column are in sequential communication, the second adapter is in the fourth position, and the sample injection member is in communication with the second one-dimensional extraction column.

6. The serum analyzer of claim 3, wherein the sample introduction member comprises an SPE pump and an autosampler, the SPE pump, the autosampler and the second adapter in turn communicating.

7. The serum analyzer of any one of claims 1 to 6, wherein the first adapter is a two-position six-way valve.

8. The serum analyzer of any one of claims 3 to 6, wherein the second adapter is a two-position, ten-way valve.

9. The serum analyzer of claim 4 or 5, wherein the second adapter is in the third position and the first adapter is in the second position with sample introduction and extraction; in the case of eluting and detecting vitamin a, the second adapter is in the fourth position and the first adapter is in the second position; in the case of capturing 25-hydroxyvitamin D, the second adapter is in the fourth position and the first adapter is in the first position; in the case of vitamin E detection and 25-hydroxyvitamin D detection, the second adapter is in the fourth position and the first adapter is in the second position.