CN102507764A - Automatic washing device and method for high-efficient liquid phase chromatograph - Google Patents
Automatic washing device and method for high-efficient liquid phase chromatograph Download PDFInfo
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- CN102507764A CN102507764A CN2011103195031A CN201110319503A CN102507764A CN 102507764 A CN102507764 A CN 102507764A CN 2011103195031 A CN2011103195031 A CN 2011103195031A CN 201110319503 A CN201110319503 A CN 201110319503A CN 102507764 A CN102507764 A CN 102507764A
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Abstract
The invention discloses an automatic washing device for a high-efficient liquid phase chromatograph. The automatic washing device comprises a high-pressure pump; an entrance of the high-pressure pump is connected with two one-position two-way electromagnetic valves through pipelines; an exit of the high-pressure pump, a sample feeder, a chromatographic column, a detector and a waste liquor bottle are sequentially connected with one another through a pipeline; the entrance of the high-pressure pump is connected with a first one-position two-way electromagnetic valve and a second one-position two-way electromagnetic valve through the pipelines; a common end and a normally-open end of the first electromagnetic valve are respectively connected to the entrance of the high-pressure pump and a mobile-phase solvent bottle through pipelines; a normally-open end and a normally-closed end of the second electromagnetic valve are respectively connected to a washing-liquor-contained solvent bottle through pipelines; and a common end of the second electromagnetic valve is connected with a normally-closed end of the first electromagnetic valve through a pipeline. With the adoption of the automatic washing device, the convenience in the washing of a system is improved, the system maintenance can be conveniently and automatically carried out, and the suitability of analytical tests can be greatly increased due to the convenient automatic washing, particularly when a high-efficient liquid phase chromatograph system with an automatic sample feeder is in long-term automatic operation.
Description
Technical field
The present invention relates to a kind of automatic flushing device and method of high performance liquid chromatograph, be particularly useful for utilizing the analytical test that contains acid, alkali and buffer salt etc. in the moving phase after system wash maintenance automatically.
Background technology
In common chemical analysis method, the analytical test that utilizes high performance liquid chromatograph to carry out has a lot of situation need add buffer reagent, for example:
In rp-hplc analysis, select correct ph value of buffer solution, very important to the reappearance of dissociable compound analysis, inappropriate pH value possibly cause dissymmetric peak, broad peak, division peak or acromion.We will discuss when using damping fluid how to select the pH value of damping fluid, and how select correct buffer system.
In reversed-phase liquid chromatography is analyzed, the pH value of moving phase generally between 2.5-7, when analyte when the pH value of dissociable under the anti-phase condition or sample is outside 2.5-7, just need damping fluid.
In Application of ion be to compositions and methods; It is similar selecting the process of damping fluid; Ion-pairing agent is the salt of TBuA for example; Myristyl sodium sulfonates etc. can match with dissociable functional group in the compound in moving phase, and the compound that in damping fluid, exists with ionic species just needs Application of ion to reagent.
If in the analytical test with containing above material in the moving phase; Common disposal route is will be earlier (or to use and analyze with moving phase in proportion with 10% methyl alcohol; Change water into containing acid, alkali, salt solusion) flushing about 1 hour, gradient is gone to 90% washed with methanol, 30～60min (if using polynary pump) again.Recycle can suitably be shortened the time several times when being necessary., after communications centre is washed, seal whole liquid-way system up for safekeeping with pure methyl alcohol or acetonitrile at last.
If operation as stated above; This just need switch the input end repeated multiple times of high performance liquid chromatograph mesohigh pump in the damping fluid of testing moving phase, flushing water, flushing organic phase and possibly using back and forth; Usual way is that the woven hose of high-pressure pump and filtering head are taken out in a liquid storage bottle of above-mentioned various solution; Put into another liquid storage bottle, pressing actual demand this process repeatedly.
This mode of operation shortcoming is following:
1) various woven hose and the filtering heads that take out and insert high-pressure pump back and forth can cause solvent that cross pollution is arranged;
2) possibly in stream, sneak into bubble in the operation that the woven hose of high-pressure pump and filtering head take out;
3) need Attended mode, inconvenient operation.
Summary of the invention
In order to remedy the deficiency of above-mentioned manual manipulation method, technical matters to be solved by this invention provides a kind of lower-cost automatic flushing device and method.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
A kind of automatic flushing attending device that is used for high performance liquid chromatograph includes injector, chromatographic column, the detecting device of high performance liquid chromatograph, and the exit of high-pressure pump, injector, chromatographic column, detecting device, waste liquid bottle connect through pipeline successively; Communication is connected between industrial control computer and driving circuit for electromagnetic valve, high-pressure pump, the detecting device; Be connected through control line between driving circuit for electromagnetic valve and two each and every one one two three-way electromagnetic valves; It is characterized in that: the porch of said high-pressure pump connects first, second two two three-way electromagnetic valves through pipeline; The common port of first solenoid valve, Chang Kaiduan are connected to the porch of high-pressure pump, the solvent bottle of moving phase through pipeline respectively; The Chang Kaiduan of second solenoid valve is connected a solvent bottle that washing fluid is housed through pipeline respectively with normal-closed end, and the common port of second solenoid valve is connected through pipeline with the normal-closed end of first solenoid valve.
Described a kind of automatic flushing attending device that is used for high performance liquid chromatograph, it is characterized in that: said solenoid valve comprises a common port, a Chang Kaiduan and a normal-closed end, when the solenoid valve no power, common port and Chang Kaiduan conducting; After solenoid valve energising work, common port and normal-closed end conducting.
Described a kind of automatic flushing attending device that is used for high performance liquid chromatograph; It is characterized in that: said industrial control computer is set the switching time point separately of two solenoid valves through software programming, after the sample analysis test; According to the program of establishment, self-stripping system.
Advantage of the present invention is: the present invention has improved the convenience of system's flushing; Make system maintenance can make things convenient for and carry out automatically; When especially operating automatically for a long time in the high performance liquid chromatograph system of configuration automatic sampler, automatic easily cleaning can increase the adaptability of analytical test greatly.
Description of drawings
Fig. 1 is system schematic of the present invention (a solid line express liquid stream, dotted line is represented cable).
Embodiment
As shown in Figure 1; The automatic flushing attending device that is used for high performance liquid chromatograph; Comprise a high-pressure pump 1; The porch of said high-pressure pump 1 connects two two three-way electromagnetic valves through pipeline, and the exit of said high-pressure pump 1, injector 4, chromatographic column 5, detecting device 6, waste liquid bottle 7 connect through pipeline successively; Be connected through cable between industrial control computer 10 and driving circuit for electromagnetic valve 11, high pressure 1 pump, the detecting device 6, be connected through cable between driving circuit for electromagnetic valve 11 and two solenoid valves.Said solenoid valve comprises a common port, a Chang Kaiduan and a normal-closed end, when the solenoid valve no power, and common port and Chang Kaiduan conducting; After solenoid valve energising work, common port and normal-closed end conducting.The common port of said first solenoid valve 2, Chang Kaiduan are connected to the porch of high-pressure pump 1, the solvent bottle 8 of moving phase through pipeline respectively; The Chang Kaiduan of second solenoid valve 3 and normal-closed end are connected through pipeline with the normal-closed end of first solenoid valve 2 through the common port that pipeline is connected solvent bottle 9, the second solenoid valves 3 of a washing fluid respectively.Said industrial control computer 10 is set the switching time point separately of two solenoid valves through software programming, after the sample analysis test, according to the program of establishment, self-stripping system.
The flow process of automatic flushing of the present invention is following: in utilizing the normal analysis of high performance liquid chromatograph; Industrial control computer 1 issues steering order to driving circuit for electromagnetic valve 11; Driving circuit for electromagnetic valve 11 controls make solenoid valve 2 no powers of winning; Its common port (COM) is connected to Chang Kaiduan (NO), and second solenoid valve 3 this moments can be in free position.The liquid road of moving phase is extracted moving phase during high-pressure pump 1 work and is carried out analytical test by gating at this moment.After analytical test is accomplished; If the moving phase of using is not suitable for chromatographic column 5 medium-term and long-term preservations, or follow-up test changes to the necessary transition of other moving phases, and the whole pipeline after will finishing to test washes; Operate as follows: industrial control computer 1 issues steering order to driving circuit for electromagnetic valve 11; Drive electricity, 11 controls make solenoid valve 2 no powers of winning, and its common port (COM) is connected to normal-closed end (NC); Second solenoid valve 3 is pressed industrial control computer Instruction Selection common port (COM) and Chang Kaiduan (NO) or normal-closed end (NC) thereby is connected and select washing fluid, extracts washing fluid during high-pressure pump 1 work and carries out the system pipeline flushing; Also can press the program behavior of industrial control computer establishment through industrial control computer programming Control second solenoid valve 3, with this select common port (COM) and Chang Kaiduan (NO) or normal-closed end (NC) thus different washing fluids is selected in connection.
Claims (3)
1. automatic flushing attending device that is used for high performance liquid chromatograph includes injector, chromatographic column, the detecting device of high performance liquid chromatograph, and the exit of high-pressure pump, injector, chromatographic column, detecting device, waste liquid bottle connect through pipeline successively; Communication is connected between industrial control computer and driving circuit for electromagnetic valve, high-pressure pump, the detecting device; Be connected through control line between driving circuit for electromagnetic valve and two each and every one one two three-way electromagnetic valves; It is characterized in that: the porch of said high-pressure pump connects first, second two two three-way electromagnetic valves through pipeline; The common port of first solenoid valve, Chang Kaiduan are connected to the porch of high-pressure pump, the solvent bottle of moving phase through pipeline respectively; The Chang Kaiduan of second solenoid valve is connected a solvent bottle that washing fluid is housed through pipeline respectively with normal-closed end, and the common port of second solenoid valve is connected through pipeline with the normal-closed end of first solenoid valve.
2. a kind of automatic flushing attending device that is used for high performance liquid chromatograph according to claim 1; It is characterized in that: said solenoid valve comprises a common port, a Chang Kaiduan and a normal-closed end; When the solenoid valve no power, common port and Chang Kaiduan conducting; After solenoid valve energising work, common port and normal-closed end conducting.
3. a kind of automatic flushing attending device that is used for high performance liquid chromatograph according to claim 1; It is characterized in that: said industrial control computer is through software programming; Set the switching time point separately of two solenoid valves; After the sample analysis test, according to the program of establishment, self-stripping system.
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| CN2011103195031A CN102507764A (en) | 2011-10-20 | 2011-10-20 | Automatic washing device and method for high-efficient liquid phase chromatograph |
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| CN2011103195031A CN102507764A (en) | 2011-10-20 | 2011-10-20 | Automatic washing device and method for high-efficient liquid phase chromatograph |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102944628A (en) * | 2012-11-28 | 2013-02-27 | 青岛轩汇仪器设备有限公司 | Ion chromatograph with automatic cleaning function |
| CN103308610A (en) * | 2012-03-08 | 2013-09-18 | 株式会社岛津制作所 | Solvent delivery device and liquid chromatograph |
| CN103969349A (en) * | 2013-01-29 | 2014-08-06 | 深圳普门科技有限公司 | A washing method of inspection equipment and the inspection equipment thereof |
| CN104690047A (en) * | 2013-12-10 | 2015-06-10 | 中国科学院大连化学物理研究所 | Liquid chromatographic sampling passage cleaning device |
| CN104950059A (en) * | 2014-03-31 | 2015-09-30 | 株式会社岛津制作所 | Liquid chromatograph |
| CN116699167A (en) * | 2023-08-08 | 2023-09-05 | 江中药业股份有限公司 | Sample injector for tight-fold combined instrument and control method thereof |
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| CN1301964A (en) * | 1999-12-28 | 2001-07-04 | 福州大学 | Intelligent integrated automatic liquid phase chromatographic system |
| CN101535815A (en) * | 2006-03-10 | 2009-09-16 | 萨瑞纳生物技术有限责任公司 | High speed sample supply device |
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2011
- 2011-10-20 CN CN2011103195031A patent/CN102507764A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1301964A (en) * | 1999-12-28 | 2001-07-04 | 福州大学 | Intelligent integrated automatic liquid phase chromatographic system |
| CN101535815A (en) * | 2006-03-10 | 2009-09-16 | 萨瑞纳生物技术有限责任公司 | High speed sample supply device |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103308610A (en) * | 2012-03-08 | 2013-09-18 | 株式会社岛津制作所 | Solvent delivery device and liquid chromatograph |
| CN103308610B (en) * | 2012-03-08 | 2015-08-05 | 株式会社岛津制作所 | Liquid feeding device and liquid chromatograph |
| US10302604B2 (en) | 2012-03-08 | 2019-05-28 | Shimadzu Corporation | Solvent delivery device and liquid chromatograph |
| CN102944628A (en) * | 2012-11-28 | 2013-02-27 | 青岛轩汇仪器设备有限公司 | Ion chromatograph with automatic cleaning function |
| CN103969349A (en) * | 2013-01-29 | 2014-08-06 | 深圳普门科技有限公司 | A washing method of inspection equipment and the inspection equipment thereof |
| CN104690047A (en) * | 2013-12-10 | 2015-06-10 | 中国科学院大连化学物理研究所 | Liquid chromatographic sampling passage cleaning device |
| CN104690047B (en) * | 2013-12-10 | 2016-08-24 | 中国科学院大连化学物理研究所 | A kind of liquid chromatograph sample intake passage cleans device |
| CN104950059A (en) * | 2014-03-31 | 2015-09-30 | 株式会社岛津制作所 | Liquid chromatograph |
| CN116699167A (en) * | 2023-08-08 | 2023-09-05 | 江中药业股份有限公司 | Sample injector for tight-fold combined instrument and control method thereof |
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Application publication date: 20120620 |